Дисертації з теми "Cell mechanobiology"
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Youngstrom, Daniel W. "Mesenchymal Stem Cell Mechanobiology and Tendon Regeneration." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/64422.
Повний текст джерелаPh. D.
Gonzalez-Molina, Jordi. "Cell-biomaterial mechanical interactions : from cancer mechanobiology to cell therapies." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10052561/.
Повний текст джерелаSmith, Rochelle. "Cancer Cell Mechanics in Chemoresistance and Chemotherapeutic Drug Exposure." Master's thesis, Faculty of Health Sciences, 2019. http://hdl.handle.net/11427/31268.
Повний текст джерелаWang, Ji. "Suspended Micro/Nanofiber Hierarchical Scaffolds for Studying Cell Mechanobiology." Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/76884.
Повний текст джерелаMaster of Science
Nichols, Anne Elizabeth Carmack. "Scleraxis-mediated regulation of tendon and ligament cell mechanobiology." Diss., Virginia Tech, 2018. http://hdl.handle.net/10919/86631.
Повний текст джерелаPh. D.
Kamble, Harshad. "Design and Development of Cell Stretching Platforms for Mechanobiology Studies." Thesis, Griffith University, 2017. http://hdl.handle.net/10072/370968.
Повний текст джерелаThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Natural Sciences
Science, Environment, Engineering and Technology
Full Text
Balachandran, Kartik. "Aortic valve mechanobiology - the effect of cyclic stretch." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/39486.
Повний текст джерелаMahajan, Gautam. "MECHANOBIOLOGY OF BRAIN-DERIVED CELLS DURING DEVELOPMENTAL STAGES." Cleveland State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=csu1578332547849308.
Повний текст джерелаMcBride, Sarah Howe. "MULTISCALE MECHANOBIOLOGY OF PERIOSTEAL BONE GENERATION: CELL SCALE STUDIES TO TRANSLATIONAL MODELS." Case Western Reserve University School of Graduate Studies / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=case1291048293.
Повний текст джерелаSharma, Puja. "A Suspended Fiber Network Platform for the Investigation of Single and Collective Cell Behavior." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/82709.
Повний текст джерелаPh. D.
Walker, Matthew. "Dynamic Mechanical Regulation of Cells in 3D Microtissues." Thesis, Université d'Ottawa / University of Ottawa, 2020. http://hdl.handle.net/10393/40555.
Повний текст джерелаGALLI, CAMILLA. "DYNAMIC INTERPLAY BETWEEN SPECTRIN, ACTIN AND PLASMA MEMBRANE DURING CELL MECHANORESPONSE." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/697440.
Повний текст джерелаLi, Tong. "Cross-scale biophysics modelling of F-actin cytoskeleton in cell." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/82293/1/Tong_Li_Thesis.pdf.
Повний текст джерелаEvans, Sarah Frances. "Top Down and Bottom Up Approaches to Elucidating Multiscale Periosteal Mechanobiology: Tissue Level and Cell Scale Studies." Case Western Reserve University School of Graduate Studies / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=case1331646902.
Повний текст джерелаLiverani, Chiara <1984>. "Investigating the Mechanobiology of Cancer Cell-ECM Interaction: The Impact of Substrate Stiffness in Breast Cancer Progression." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2018. http://amsdottorato.unibo.it/8308/7/Tesi%20dottorato%20Liverani.pdf.
Повний текст джерелаEsmaeili, Pourfarhangi Kamyar. "Movie1: MTLn3 cell switching from Migration to Invadopodia state." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584756.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
McKenzie, Andrew J. "Mechanoregulation of leading edge PKA activity during ovarian cancer cell migration." ScholarWorks @ UVM, 2014. http://scholarworks.uvm.edu/graddis/273.
Повний текст джерелаEsmaeili, Pourfarhangi Kamyar. "Movie2: MDA-MB-231 cell switching between Migration and Invadopodia states." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584754.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Sheets, Kevin Tyler. "Cell-Fiber Interactions: A New Route to Mechano-Biological Investigations in Developmental and Disease Biology." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/70855.
Повний текст джерелаPh. D.
Xu, Zhenyuan. "The Role of the Extracellular Matrix in Schwann Cell Phenotype." University of Cincinnati / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1623251473003085.
Повний текст джерелаGnanasambandam, Bhargavee. "Design of Modified Traction Force Microscopy for Cell Response to De Novo ECM." Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1591698756910799.
Повний текст джерелаMartin, Madge Audrey Marie. "Bone remodelling and mechanomics: Bridging organ, tissue, and cell scales to understand bone structure and function." Thesis, Queensland University of Technology, 2020. https://eprints.qut.edu.au/203101/1/Madge%20Audrey%20Marie%20Martin%20Thesis.pdf.
Повний текст джерелаEsmaeili, Pourfarhangi Kamyar. "Movie10: Computational image segmentation and tracking performed by LEVER." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584746.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie12: FUCCI-MDA-MB-231 cells migration in 3D collagen with vertically aligned fiber architecture." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584747.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie3: Oscillations of cortactin fluorescent signal in invadopodia." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584749.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie8: FUCCI-MDA-MB-231 cells migration on 2D gelatin." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584750.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie4: Absence of cortactin oscillations upon F-actin polymerization inhibition by Cytochalasin." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584751.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie14: A HS-578T cells migration in the orthogonal dual-cue condition." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584752.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie5: Dynamics of calcium spikes measured by Fluo-4-AM." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584753.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie13: HS-578T cells migration in the orthogonal dual-cue condition." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584755.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie11: FUCCI-MDA-MB-231 cells migration in 3D collagen with random fiber architecture." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584757.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie7: Simulation of EGF diffusion within the microchannels." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584758.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie9: FUCCI-MDA-MB-231 cells migration inside the microchannels." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584759.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Movie6: Dynamics of MT1-MMP vesicle delivery to the plasma membrane." Diss., Cancer Invasion; Cell Migration; Chemotaxis; Contact Guidance; Invadopodia; Mechanobiology, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/584760.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Esmaeili, Pourfarhangi Kamyar. "Effect of Extrinsic and Intrinsic Factors on Cancer Invasion." Diss., Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/585155.
Повний текст джерелаPh.D.;
Metastasis is the leading cause of death among cancer patients. The metastatic cascade, during which cancer cells from the primary tumor reach a distant organ and form multiple secondary tumors, consists of a series of events starting with cancer cells invasion through the surrounding tissue of the primary tumor. Invading cells may perform proteolytic degradation of the surrounding extracellular matrix (ECM) and directed migration in order to disseminate through the tissue. Both of the mentioned processes are profoundly affected by several parameters originating from the tumor microenvironment (extrinsic) and tumor cells themselves (intrinsic). However, due to the complexity of the invasion process and heterogeneity of the tumor tissue, the exact effect of many of these parameters are yet to be elucidated. ECM proteolysis is widely performed by cancer cells to facilitate the invasion process through the dense and highly cross-linked tumor tissue. It has been shown in vivo that the proteolytic activity of the cancer cells correlates with the cross-linking level of their surrounding ECM. Therefore, the first part of this thesis seeks to understand how ECM cross-linking regulates cancer cells proteolytic activity. This chapter first quantitatively characterizes the correlation between ECM cross-linking and the dynamics of cancer cells proteolytic activity and then identifies ß1-integrin subunit as a master regulator of this process. Once cancer cells degrade their immediate ECM, they directionally migrate through it. Bundles of aligned collagen fibers and gradients of soluble growth factors are two well-known cues of directed migration that are abundantly present in tumor tissues stimulating contact guidance and chemotaxis, respectively. While such cues direct the cells towards a specific direction, they are also known to stimulate cell cycle progression. Moreover, due to the complexity of the tumor tissue, cells may be exposed to both cues simultaneously, and this co-stimulation may happen in the same or different directions. Hence, in the next two chapters of this thesis, the effect of cell cycle progression and contact guidance-chemotaxis dual-cue environments on directional migration of invading cells are assessed. First, we show that cell cycle progression affects contact guidance and not random motility of the cells. Next, we show how exposure of cancer cells to contact guidance-chemotaxis dual-cue environments can improve distinctive aspects of cancer invasion depending on the spatial conformation of the two cues. In this dissertation, we strive to achieve the defined milestones by developing novel mathematical and experimental models of cancer invasion as well as utilizing fluorescent time-lapse microscopy and automated image and signal processing techniques. The results of this study improve our knowledge about the role of the studied extrinsic and intrinsic cues in cancer invasion.
Temple University--Theses
Manifacier, Ian. "Understanding adherent cell mechanics and the influence of substrate rigidity." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM4106/document.
Повний текст джерелаTissue engineering is a medical strategy based on utilizing cells and materials to regenerate a new tissue. Yet, it involves intertwined interactions that allow cells to act as integrated parts of an organ. In addition to chemical reactions, the cell interacts mechanically with its environment by sensing its rigidity. Here, we used several computational models to understand how substrate rigidity affects a cell’s structure as it adheres and spreads on it. In other words we tried to understand the way a cell feels how soft or hard it surrounding is, how it affects its internal structure and the forces that transit within it. In addition, instead of focusing on mechanical properties, we developed a simplified, yet coherent conceptual understanding of the cellular structure
Gaut, Ludovic. "Mechanical and molecular signals underlying tendon cell differentiation." Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS301.
Повний текст джерелаTendons are unique forms of connective tissue of the musculoskeletal system. Tendon development, homeostasis and repair rely on specific combinations of mechanical and molecular factors regulating the production and assembly of collagen fibers. Our objective is to decipher the mechanotransduction pathways underlying tendon cell differentiation, through the activity of two transcription (co-)factors, EGR1 and YAP. We showed that the expression of the tendon gene SCX, the mechanosensitive gene EGR1 and YAP activity were downregulated in limb tendons of immobilized chicken fetuses. Restored muscle contraction after immobilization led to a recovery of tendon gene expression. Tendon mechanobiology was studied in vitro in fibrin- or collagen-based 3-dimensional (3D) constructs made of mesenchymal stem cells and mimicking tendon formation. Tension release in fibrin and collagen 3D-constructs induced a drop of the expression of Egr1, tendon genes and YAP activity. Overexpression of Egr1 was able to prevent the downregulation of tendon gene expression in de-tensioned fibrin 3D-constructs. YAP activity was upregulated in dynamically stretched collagen 3D-constructs and was paired with the expression of the tendon gene Scx. Chemical knock-down of YAP activity with Verteporfin (VTPF) treatment showed a decrease in the expression of YAP target genes and the tendon genes. Besides, dynamic stretch applied on VTPF-treated constructs did not restore tendon gene expression, conforting the role of YAP as an intracellular relay of mechanical cues in tendon cells. Altogether, these results highlight the importance of EGR1 and YAP downstream of mechanical forces during tendon cell differentiation
McKenty, Taylor R. "QUANTIFYING THE EFFECTS OF HYDROSTATIC PRESSURE ON FIBROBLAST GROWTH FACTOR-2 BINDING BY THE HUMAN ENDOTHELIUM." UKnowledge, 2017. http://uknowledge.uky.edu/cbme_etds/47.
Повний текст джерелаWang, Qian. "Elastomer-based Cellular Micromechanical Stimulators for Mechanobiological Study." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1397610258.
Повний текст джерелаDesvignes, Emma. "Dispositifs fluidiques 3D pour l'étude de la migration cellulaire des macrophages." Thesis, Toulouse, INSA, 2018. http://www.theses.fr/2018ISAT0046.
Повний текст джерелаOver the past two decades, studies have been conducted to measure the mechanical forces exerted by living cells on their environment. This has led to the development of a variety of ingenious techniques that have been primarily developed to understand how cells exert forces during their migration on 2D substrates. However, in vivo, cells migrate through three-dimensional (3D) environments and the mechanisms used to migrate in 3D differ significantly from those of 2D migration. For example, confined cells in 3D encountering constrictions need to deform their nucleus, their largest and most rigid organelle. In 2D, kernels are not limiting factors for migration. It is therefore necessary to develop tools to understand how cells migrate in 3D. In particular, studies need to be conducted to determine how cells apply forces based on the level of containment they encounter. To answer this difficult question, we have developed two types of micro-devices. First, we designed and manufactured a microfluidic device to study the forces generated by cells during a confined migration. This device consists of microchannels of controlled dimensions equipped with micropiliers, serving as force sensors. These force sensors have a sensitivity of the order of 70 pN. We then introduced into the device human macrophages, cells of the immune system, inside the device and evaluated the bending of micropiliers generated by the cellular forces applied during their migration. Through the development of an algorithm for image analysis, we have been able to evaluate the forces generated in different cell areas and reveal that cells are redirecting pressure forces from the inside to the outside as the degree of containment increases. This observation suggests a very specific mode of migration related to spatial confinement that is based on the support without adhesion on the obstacles of the environment. In a second time we made three-dimensional networks obtained by a 3D bi-photonic lithography method. Les motifs de ces réseaux possèdent des dimensions caractéristiques de l'échelle cellulaire (1-10 μm) et sont composés de poutres suspendues qui peuvent être courbés par les cellules vivantes qui migrent au sein du treillis tri-dimensionnel. En enregistrant une séquence vidéo des déformations de l'échafaudage, nous pouvons étudier l'activité mécanique de la cellule dans l'espace et le temps pendant sa migration 3D. Nos résultats montrent que les macrophages sont capables de pénétrer dans des réseaux de géométrie cubique lorsque la période du réseau est supérieure à 5 μm et que le support de migration lui-même peut être utilisé comme capteur pour mesurer les forces exercées par les cellules. Grâce à la mesure de la rigidité du matériau constituant le treillis 3D et des modélisations de la déformation élastique de la structure 3D, nous avons pu évaluer que la contrainte mécanique globale qu’exerce un macrophage sur son microenvironnement est de l’ordre de 500 kPa. Grâce à la combinaison de la microfabrication, l'imagerie cellulaire et l'analyse automatisée des images, nous sommes parvenus à quantifier les efforts mécaniques cellulaires mis en jeu lors de la migration de macrophages humains au sein d’environnements confinés et nous mettons ainsi en lumière la mécanique spécifique des cellules migrant en 3D
Fell, Cody Alexander. "Soft robotic devices for emulating vascular mechanobiology." Thesis, Queensland University of Technology, 2021. https://eprints.qut.edu.au/213109/1/Cody%20Alexander_Fell_Thesis.pdf.
Повний текст джерелаSotto, David C. "Directing the migration of mesenchymal stem cells with superparamagnetic iron oxide nanoparticles." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/54897.
Повний текст джерелаKhalil, Georges. "Modeling of the bone-implant healing : mechanobiology of osteoblasts population in presence of endothelial cells." Toulouse 3, 2011. http://thesesups.ups-tlse.fr/1441/.
Повний текст джерелаThe long term survival of arthoplasty is strongly related to the quality of the immediative post-operative healing of periprosthetic tissue. Despite good clinical results, revisions are increasing (hip, knee, shoulder). The central hypothesis of this work was that modeling of mechanobiological interactions and neo-vascularization into biological tissues of periprosthetic zone might help to investigate the multifactorial events in tissue healing. The methodology of reactive transports in deformable porous media has been associated to computational cell biology (cellular migration and proliferation in presence of anabolic growth factors). The populations of osteoblasts and endothelial cells, the phases of bone growth factors, angiogenic factors and fibronectin have taken into account to propose a set of governing equations describing the process of intramembranous healing. The application concerned a reference experimental implant (canine micromotion implant) developed within the framework of an international collaboration (K. Søballe - Denmark, J. E. Bechtold - USA). The spatio-temporal resolution was achieved by using the finite element method (mixed formulation displacement-pressure) implemented into COMSOL Multiphysics(r). Parametric sensitivity studies were implemented to study the role of mechanical instability of implant and the supply from the neo-vascularization of the periprosthetic zone. The results were in good agreement with the experimental database from the canine experimental model and clinical observations. This confirmed the potential interests of our original methodology
Rabineau, Morgane. "Influence de l'élasticité du substrat sur la plasticité de la chromatine de cellules épithéliales et sur la division de cellules tumorales." Thesis, Strasbourg, 2013. http://www.theses.fr/2013STRAJ079/document.
Повний текст джерелаIn the biomaterials field, this PhD work is about influence of substrate elasticity on cell division and chromatin plasticity of epithelial cells. Soft substrates cause massive death.However, some SW480 tumor cells, including those bearing chromosomal segregation abnormalities progress in mitosis. These abnormalities could result in more chromosomal rearrangements, increasing mutations. Soft substrates lead to heterochromatin remodelling and very soft substrates promote necrosis of PtK2 cells. On these substrates, euchromatin could be maintained after HDAC inhibition independently of the nuclear transcriptional competence.These cells spread again after tranfer on stiff substrates. These results suggest i) outside-insignalling cascade initiated at the soft substrate surface leading to heterochromatin remodelling and ultimately necrosis, ii) inside-out signaling cascade initiated from euchromatin allowing cell to overcome necrosis on soft substrate
Gilbert, Hamish. "The response of human annulus fibrosus cells to cyclic tensile strain : evidence for an altered mechanotransduction pathway with intervertebral disc degeneration." Thesis, University of Manchester, 2011. https://www.research.manchester.ac.uk/portal/en/theses/the-response-of-human-annulus-fibrosus-cells-to-cyclic-tensile-strain-evidence-for-an-altered-mechanotransduction-pathway-with-intervertebral-disc-degeneration(e61ab359-7b23-454e-ab4b-2236d3ea9ed9).html.
Повний текст джерелаBrunelli, Marzia. "A mechanobiology study on the response to mechanical compression of mesenchymal progenitor cells cultured in a composite scaffold made of 3D Insert PCL and collagen gel." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/12767/.
Повний текст джерелаChagnon-Lessard, Sophie. "Cellular Responses to Complex Strain Fields Studied in Microfluidic Devices." Thesis, Université d'Ottawa / University of Ottawa, 2018. http://hdl.handle.net/10393/37915.
Повний текст джерелаDoyle, Adele Marion. "Conservation of mechanosignaling: responses of human adult mesenchymal stem cells and differentiated vascular cells to applied physical forces." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/39526.
Повний текст джерелаJaddivada, Siddhartha. "Mechanobiology of cell-substrate interactions." Thesis, 2022. https://etd.iisc.ac.in/handle/2005/5729.
Повний текст джерелаPfeiler, Terry Wayne. "Computational and experimental analyses of bone and adult stem cell mechanobiology." 2009. http://www.lib.ncsu.edu/theses/available/etd-11032009-064954/unrestricted/etd.pdf.
Повний текст джерела