Дисертації з теми "Cell culture processing"
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Luhr, Katarina. "Prion processing and propagation in neuronal and dendritic cell culture models /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7349-991-9.
Повний текст джерелаWhite, Nicole M. "Inherent Flaw of Cholesterol Processing in Cell Culture and In Vivo Models of Cystic Fibrosis." Case Western Reserve University School of Graduate Studies / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=case1164925733.
Повний текст джерелаDubé, Gilles. "Post-translational processing of atrial natriuretic factor. A study using a novel cell culture system." Thesis, University of Ottawa (Canada), 1992. http://hdl.handle.net/10393/7779.
Повний текст джерелаTapia, Delgado Felipe Ignacio [Verfasser], and Udo [Gutachter] Reichl. "Continuous upstream processing for cell culture-derived virus production / Felipe Ignacio Tapia Delgado ; Gutachter: Udo Reichl." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2020. http://d-nb.info/1219966479/34.
Повний текст джерелаTapia, Delgado Felipe Ignacio Verfasser], and Udo [Gutachter] [Reichl. "Continuous upstream processing for cell culture-derived virus production / Felipe Ignacio Tapia Delgado ; Gutachter: Udo Reichl." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2020. http://d-nb.info/1219966479/34.
Повний текст джерелаWheater, R. F. "The development of an in vitro system for an electron microscope study of procollagen processing in cell culture." Thesis, University of Manchester, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376285.
Повний текст джерелаDebavelaere, Dorothée. "Caractérisation de progéniteurs osseux en culture, développement d'une technique ultrasonore d'analyse dynamique." Valenciennes, 1996. https://ged.uphf.fr/nuxeo/site/esupversions/155642e1-ddd5-4273-99c5-ea8f7bf9663f.
Повний текст джерелаMEYER, ANSTETT COLETTE. "Analyse d'images de cellules en culture : description automatique de cellules musculaires lisses vasculaires et suivi de leur comportement." Université Louis Pasteur (Strasbourg) (1971-2008), 1986. http://www.theses.fr/1986STR13063.
Повний текст джерелаDempsey, Katherine. "Monitoring individual cells within cell cultures using image processing and pattern recognition techniques." Thesis, Keele University, 2017. http://eprints.keele.ac.uk/4179/.
Повний текст джерелаBeck, Mike. "Molekulare Charakterisierung des Amyloidvorläuferproteins des Meerschweinchens." Doctoral thesis, Universitätsbibliothek Leipzig, 2004. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-36500.
Повний текст джерелаA beta peptides, the major component of neuritic plaques found in the brains of patients with Alzheimers disease, are derived by proteolytic processing from a larger precursor molecule (amyloid precursor protein - APP). A combination of PCR methods was used to clone and sequence APP cDNA from guinea pig (Cavia porcellus). Guinea pig APP exhibits extensive similarities to human APP in terms of primary structure, mRNA expression of differentially spliced isoforms as shown by Northern blot and RT-PCR analysis as well as proteolytic processing to amyloidogenic A beta peptides. In contrast to rat and mouse APP, guinea pig APP - recombinantly expressed in human neuroblastoma-cells - was processed indistinguishable from human APP thus excluding intrinsic sequence-specific factors influencing processing. Further studies were performed using newly established primary cell cultures of guinea pig neurons. Refined methods have been used to detect and characterize major proteolytic processing products of APP in vitro and in vivo. In conclusion, guinea pigs provide a model to study expression and processing of APP that closely resembles the physiological situation in humans and should, therefore, be important in elucidating potential strategies to prevent amyloid formation in Alzheimers Disease
Vlachynská, Alžběta. "Metody pro obrazovou analýzu populace fotosyntetických buněčných kultur." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2015. http://www.nusl.cz/ntk/nusl-221387.
Повний текст джерелаBradhurst, Christopher John. "Monitoring mesenchymal stem cell cultures using image processing and pattern recognition techniques." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/43623/1/Christopher_Bradhurst_Thesis.pdf.
Повний текст джерелаBowes, Simone. "Processing of Alzheimer's amyloid precursor protein in cultured cells." Thesis, Sheffield Hallam University, 1999. http://shura.shu.ac.uk/19377/.
Повний текст джерелаHameed, Radwan [Verfasser]. "Analysis of lipid uptake and processing in cultured cells / Radwan Hameed." Bonn : Universitäts- und Landesbibliothek Bonn, 2013. http://d-nb.info/1044868244/34.
Повний текст джерелаJaccard, N. "Development of an image processing method for automated, non-invasive and scale-independent monitoring of adherent cell cultures." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1461036/.
Повний текст джерелаBuergy, Alexandra. "Modulation de la texture et de la fragmentation tissulaire de fruits lors de traitements thermiques par les modes de culture et la maturation : impact sur la texture des purées Pectin modifications in raw fruits alter texture of plant cell dispersions Apple puree’s texture is independent from fruit firmness Pectin degradation explains tissue fragmentation of fruits during thermomechanical processes for puree production." Thesis, Avignon, 2021. http://www.theses.fr/2021AVIG0282.
Повний текст джерелаThe objective of this thesis was to understand how structural characteristics in raw apples can be linked to structural factors in purees after cooking and tissue fragmentation. Structural characteristics of the fruit were modulated by cultivars, agricultural practices and maturation, and process conditions (thermal: 50–95 °C and mechanical: 100–3000 rpm) were modulated in a cooker-cutter during processing. Puree’s structure (volume occupied by particles, particle size, serum viscosity) and texture (viscosity, yield stress, G’ and G’’) were then analysed and compared between raw materials and process conditions. Pectins were extracted and their chemical composition and structure were correlated to puree’s structure. Particle size appeared to be the most important determinant of puree’s texture when there is no dilution or concentration of the fruit tissue. The extent of cell adhesion (defined by pectin structure and composition) determined particle size more than individual cell size (defined by varietal effects or agricultural practices). Other structural factors only contributed to puree’s texture once particle size was constant. Tissue fragmentation, determining particle size during processing, was principally affected by shear intensity. Post-harvest maturity of the raw apples and high temperatures (95 °C) induced pectin degradation, especially rhamnogalacturonan I side chain hydrolysis, and solubilisation. This led to reduced cell adhesion and tissue fragmentation was additionally favoured. The results deepened the understanding of tissue fragmentation and textural changes during processing and provided guidelines for industry to manage diversity and heterogeneity of raw fruits during processing
Rowlands, R. J. "The effects of statin treatment on the synthesis, processing, storage and exocytosis of von Willebrand factor in cultured human endothelial cells." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1306806/.
Повний текст джерелаBoussemaere, Luc. "Investigating off-axis digital holographic microscopy with a source of partial spatial coherence as a real-time sensor for cell cultures." Doctoral thesis, Universite Libre de Bruxelles, 2015. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209086.
Повний текст джерелаDoctorat en Sciences de l'ingénieur
info:eu-repo/semantics/nonPublished
Hadpe, Sandeep Tamesh. "Using experimental and theoretical appoaches for understanding biotechnology unit operations." Thesis, 2017. http://localhost:8080/xmlui/handle/12345678/7454.
Повний текст джерела"CAD/CAM laser processing as a method for integrated fabrication of microphysiological systems." Tulane University, 2020.
Знайти повний текст джерелаWu, Cheng-Chi, and 吳振棋. "The effects and applications of ultrafast laser pulses on the growth of cell culture and the processing of multiwall carbon nanotubes." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/33ewt8.
Повний текст джерела國立陽明大學
生醫光電工程研究所
97
The reported study in this thesis is twofold. The research work is aimed to investigate the effect of ultrafast laser on multiwall carbon nanotubes through ablation and on cell culture through laser induced shockwave. In the ablation based material processing, nano- and femtosecond laser pulses are focused to engrave fine structures on multi-layer carbon nanotube coated surface. The effects of ablation are compared. We found that both nano- and femtosecond laser pulses produced approximately the same spatial resolution or definition. Femtosecond laser pulses, however, is able to generate highly clean-cut features without much residue. For comparison, more residue were found with nanosecond laser pulses Laser-induced shockwave and cavitation is also used to generate mechanical stress on living cultured cells. We found that the laser-induced mechanical stress strongly affected the cultured cells by severely limiting or stimulating the growth, depending on the types of the cells. The growth of P19CL6 (Embryonal carcinoma) and C2C12 (muscle) cell cultures is greatly stimulated, while the growth of PC12 (Pheochromocytoma) and HeLa (cervix) is severely inhibited. Very interesting interpretation is thus implied by the above results and forms the direction of future investigation.
Capela, Emanuel Augusto Vieira. "Innovative and sustainable platforms for the downstream processing of antibody-based biopharmaceuticals." Doctoral thesis, 2022. http://hdl.handle.net/10773/33484.
Повний текст джерелаOs anticorpos, e em particular a imunoglobulina G (IgG), são considerados uma das pontas de lança da indústria biofarmacêutica, apresentando elevada relevância para o tratamento de várias doenças e sendo, por vezes, a única terapia disponível para algumas patologias. Apesar do seu amplo potencial, a extração e purificação destas biomoléculas a partir dos seus meios biológicos complexos com elevada qualidade e pureza é ainda baseada em abordagens com várias etapas e com elevado custo associado. Portanto, o desenvolvimento de processos a jusante alternativos, económicos e eficientes, capazes de fornecer elevadas quantidades de anticorpos terapêuticos a um custo reduzido é altamente necessário. Novas estratégias baseadas em líquidos iónicos (LIs) foram então investigadas nesta tese de Doutoramento para o processamento a jusante de anticorpos. Os LIs foram escolhidos principalmente devido ao seu carácter de solventes customizáveis. Esta característica dos LIs permite adequar a polaridade, interações e seletividade dos processos desenvolvidos, permitindo superar algumas limitações técnicas dos processos convencionais. Três tipos de plataformas baseadas em LIs foram investigadas para o processamento a jusante de anticorpos, nomeadamente sistemas aquosos bifásicos (SAB), partição trifásica e líquidos iónicos suportados. SAB contendo LIs como adjuvantes foram investigados, permitindo a extração e purificação de anticorpos humanos (anticorpos policlonais e monoclonais) com um bom desempenho em uma única etapa. LIs mais biocompatíveis e sustentáveis foram também estudados como compostos formadores de fases de SAB, demonstrando em simultâneo a possibilidade de utilizar abordagens de partição trifásica baseadas em SAB para a purificação e recuperação de anticorpos humanos. Finalmente, foram propostas novas matrizes cromatográficas baseadas em líquidos iónicos suportados, capazes de capturar e/ou purificar anticorpos a partir dos seus meios biológicos complexos através de dois mecanismos distintos, nomeadamente através dos modos negativo e positivo. Em suma, nesta tese de Doutoramento foi demonstrado que os LIs, se adequadamente concebidos, podem ser aplicados com sucesso na extração, purificação e/ou recuperação de anticorpos humanos, sendo plataformas alternativas promissoras para o processamento a jusante de biofármacos baseados em anticorpos.
Programa Doutoral em Engenharia Química
Beck, Mike. "Molekulare Charakterisierung des Amyloidvorläuferproteins des Meerschweinchens." Doctoral thesis, 1998. https://ul.qucosa.de/id/qucosa%3A10869.
Повний текст джерелаA beta peptides, the major component of neuritic plaques found in the brains of patients with Alzheimers disease, are derived by proteolytic processing from a larger precursor molecule (amyloid precursor protein - APP). A combination of PCR methods was used to clone and sequence APP cDNA from guinea pig (Cavia porcellus). Guinea pig APP exhibits extensive similarities to human APP in terms of primary structure, mRNA expression of differentially spliced isoforms as shown by Northern blot and RT-PCR analysis as well as proteolytic processing to amyloidogenic A beta peptides. In contrast to rat and mouse APP, guinea pig APP - recombinantly expressed in human neuroblastoma-cells - was processed indistinguishable from human APP thus excluding intrinsic sequence-specific factors influencing processing. Further studies were performed using newly established primary cell cultures of guinea pig neurons. Refined methods have been used to detect and characterize major proteolytic processing products of APP in vitro and in vivo. In conclusion, guinea pigs provide a model to study expression and processing of APP that closely resembles the physiological situation in humans and should, therefore, be important in elucidating potential strategies to prevent amyloid formation in Alzheimers Disease.