Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: CDFII.
Статті в журналах з теми "CDFII"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 статей у журналах для дослідження на тему "CDFII".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте статті в журналах для різних дисциплін та оформлюйте правильно вашу бібліографію.
1
Moed, S. "Top Production and Properties at CDFII." Journal of Physics: Conference Series 110, no. 4 (May 1, 2008): 042016. http://dx.doi.org/10.1088/1742-6596/110/4/042016.
Повний текст джерела
2
FARRINGTON, SINÉAD M. "RECENT RESULTS IN b QUARK PHYSICS AT CDF." International Journal of Modern Physics A 23, no. 21 (August 20, 2008): 3309–13. http://dx.doi.org/10.1142/s0217751x08042031.
Повний текст джерела
3
Hill, Christopher S. "Operational experience and performance of the CDFII silicon detector." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 530, no. 1-2 (September 2004): 1–6. http://dx.doi.org/10.1016/j.nima.2004.05.037.
Повний текст джерела
4
Affolder, A., A. Barbaro-Galtieri, A. Connolly, C. Haber, F. Zetti, N. Bacchetta, D. Bisello, et al. "Status report of the intermediate silicon layers detector at CDFII." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 485, no. 1-2 (June 2002): 6–9. http://dx.doi.org/10.1016/s0168-9002(02)00524-7.
Повний текст джерела
5
Affolder, A., P. Azzi-Bacchetta, N. Bacchetta, A. Barbaro-Galtieri, A. Basti, F. Bedeschi, D. Bisello, et al. "The intermediate silicon layers detector at CDFII: Design and progress." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 435, no. 1-2 (October 1999): 44–50. http://dx.doi.org/10.1016/s0168-9002(99)00408-8.
Повний текст джерела
6
Hartz, M. "Radiation damage studies and lifetime estimates for the CDFII silicon detector." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 583, no. 1 (December 2007): 23–26. http://dx.doi.org/10.1016/j.nima.2007.08.200.
Повний текст джерела
7
Giagu, Stefano. "The CDFII Time-of-Flight detector and impact on beauty flavor tagging." Nuclear Physics B - Proceedings Supplements 120 (June 2003): 219–24. http://dx.doi.org/10.1016/s0920-5632(03)01905-4.
Повний текст джерела
8
Trovato, Marco, and Caterina Vernieri. "A Novel Technique to Reconstruct the Z mass in WZ/ZZ Events with Lepton(s), Missing Transverse Energy and Three Jets at CDFII." EPJ Web of Conferences 28 (2012): 12023. http://dx.doi.org/10.1051/epjconf/20122812023.
Повний текст джерела
9
Ito, Zensho, Kan Uchiyama, Shunichi Odahara, Shinichiro Takami, Keisuke Saito, Hiroko Kobayashi, Shigeo Koido, Takahiro Kubota, Toshifumi Ohkusa, and Masayuki Saruta. "Fatty Acids as Useful Serological Markers for Crohn’s Disease." Digestive Diseases 36, no. 3 (December 22, 2017): 209–17. http://dx.doi.org/10.1159/000485096.
Повний текст джерелаАнотація:
Background: We have previously reported that patients with Crohn’s disease (CD) have a very specific erythrocyte membrane phospholipid fatty acid profile. The findings of this study suggest that the activities of enzymes involved in the metabolism of linoleic acid (LA), that is, delta-6 desaturase, are higher in CD patients than in healthy individuals. Methods: We evaluated the utilities of various fatty acid compositions of the plasma (p-) as new serological markers for CD compared to those of erythrocyte membranes (e-). Results: Fifty CD patients and 50 healthy individuals were enrolled. In both plasma and erythrocyte membranes, the weight percentages of palmitic acid (PA) were significantly higher, while those of LA were significantly lower in CD patients than in controls. Fatty acids with high sensitivity and specificity were p-PA (0.86 and 0.74) and e-PA (0.80 and 0.74). With PA and LA as a CD fatty acid index (CDFAi), that is, CDFAi = (PA/LA), the sensitivity and specificity of plasma CDFAi (p-CDFAi) and e-CDFAi were 0.80 and 0.80; and 0.82 and 0.88 respectively. Conclusion: In CD patients, various fatty acids were specifically altered in both plasma and erythrocytes, and p-PA and p-CDFAi are potentially useful as new serological markers for CD.
10
Hu, Fu, Qian Li, Benwei Zhu, Fang Ni, Yun Sun, and Zhong Yao. "Effects of module truncation on biochemical characteristics and products distribution of a new alginate lyase with two catalytic modules." Glycobiology 29, no. 12 (May 18, 2019): 876–84. http://dx.doi.org/10.1093/glycob/cwz064.
Повний текст джерелаАнотація:
Abstract In this work, we investigated the functions of structural modules within alginate lyase by truncating an endo-type alginate lyase into two successive catalytic modules. The effects of module deletion on biochemical characteristics and product distributions were further investigated. The N-terminal module (Aly7B-CDI) exhibited no activity toward alginate, polyM or polyG, but the C-terminal module (Aly7B-CDII) retained its activity. The full-length enzyme (Aly7B) and its truncated counterpart (Aly7B-CDII) had similar substrate specificities, but Aly7B-CDII had lower activity. Moreover, the activity of Aly7B was much higher than Aly7B-CDII at 30°C. Aly7B-CDII, however, possessed higher optimal pH and better pH stability than the full-length enzyme. The final degradation products for Aly7B were unsaturated di-, tri- and tetra-oligosaccharides, and those for Aly7B-CDII were unsaturated mono-, di-, tri-, tetra- and penta-oligosaccharides. Therefore, the potential impact of the noncatalytic module Aly7B-CDI on the catalytic module Aly7B-CDII was further elucidated by characterizing Aly7B and its truncations. These data contribute to the functional understanding of these differing modules.
11
Wang, Guang Hui, Da Jun Song, Hong Xiao Tian, Jian Gao, and Yu Liu. "Structure Studies on Order Assemblage of CadmiumII Isophthalicacid Benzimidazole Complexes." Applied Mechanics and Materials 713-715 (January 2015): 2872–75. http://dx.doi.org/10.4028/www.scientific.net/amm.713-715.2872.
Повний текст джерелаАнотація:
The CadmiumII Isophthalicacid Benzimidazole complex [Cd2(C8H4O4)2 (C7H6N2)4(H2O)3], Triqua-bis (isophthalicacidato-O,O')-tetra (benzimidazole) bis (CadmiumII),the CdII atom is coordinated by two isophthalicacid anions and two benzimidazole and one water molecule in a distorted octahedral configuration with six-coordinations geometry. And one isophthalicacid ligand chelates to the CdII atom through its one carboxylic O atoms, but the other isophthalicacid ligand chelates to the CdII atom through its two carboxylic O atoms. The same as another CdII atom. So we get a Binuclear CdII metal complexe. The fact clearly suggests not so much significant contribution from the electrostatic interaction in the Cd-O bonding in bidentate Binuclear CdII metal complexes as we gotten in mononuclear MnII metal complexes. Adjacent complex link to each other via hydrogen bonds forming the three-dimensional supramolecular structure.
12
Pham Tien, Nam, Trang Nguyen Thu, and Huong Tieu Thi Minh. "PROBLEMS IN COMMUNITY DEVELOPMENT FIELD INSTRUCTION PROGRAM AMONG SOCIAL WORK STUDENTS IN HANOI, VIETNAM." Journal of Science Social Science 65, no. 11 (November 2020): 110–22. http://dx.doi.org/10.18173/2354-1067.2020-0076.
Повний текст джерелаАнотація:
Social work students are coping with challenges in the community development field instruction (CDFI) program. Therefore, this study aims to find out problems in CDFI program among social work students in Hanoi, Vietnam. The total number of quantitative study participants was 30, and eight (8) social work students participated in in-depth interviews. Our results showed that problems in the CDFI program include personal, community, and supervision problems. These problems posed a challenge to social work students. We had suggestions to stakeholders to improve CDFI program.
13
Mcgeehan, Sarah. "CDFIs—Coming of Age?" Local Economy: The Journal of the Local Economy Policy Unit 21, no. 1 (February 2006): 84–90. http://dx.doi.org/10.1080/02690940500472582.
Повний текст джерела
14
Bianchi, Paola, Klaus Schwarz, Elisa Fermo, Katja Heinrich, Cristina Vercellati, Anna Paola Maria Luisa Marcello, Richard van Wijk, et al. "Molecular Analysis of the SEC23B Gene In Patients Affected by Congenital Dyserythropoietic Anemia Type II (CDAII)." Blood 116, no. 21 (November 19, 2010): 4227. http://dx.doi.org/10.1182/blood.v116.21.4227.4227.
Повний текст джерелаАнотація:
Abstract Abstract 4227 CDAII, the most frequent type of congenital dyserythropoietic anemia, is an autosomal recessive disease characterized by ineffective erythropoiesis, peripheral hemolysis, erythroblast morphological abnormalities and hypoglycosylation of some RBC membrane proteins. Last year we and others identified SEC23B as the gene responsible for CDAII (Schwarz et al, 2009, Bianchi et al, 2009). SEC23B is a member of the SEC23/SEC24 family, a component of COPII coat protein complex which is involved in protein trafficking through membrane vesicles from the endoplasmic reticulum to the Golgi apparatus. The gene, localized on chromosome 20p11, is split in 20 exons and codifies for a 767 aa protein. The aim of the study was to characterize the molecular defect in a large series of CDAII patients of Caucasian origin. Fifty-one CDAII patients from 49 unrelated families (17 Italians, 20 German or Swiss, 4 Dutch, 2 British, 2 Czech and 1 Greek, Turkish, Bulgarian and Irish origin) were analyzed by direct exon sequencing. We identified 36 different mutations, 25 of which were not described before. Eighteen were disruptive and 18 were missense mutations. The patients' molecular data are summarized in the Figure (novel mutations are reported in bold). All the missense mutations affected highly conserved aminoacids and were not found in 200 normal alleles examined. The c.325G>A mutation was identified in 15 homozygous patients and in two cases in combination with other mutations. The change c.40C>T was detected in 16 unrelated patients as heterozygous mutation and, for the first time, at the homozygous level in one patient only. Considering the entire series of patients (including previously published cases) characterized by our groups (86 CDAII patients from 77 unrelated families) c.325G>A and c.40C>T mutations account for 49% (76/154) of the unrelated mutated alleles and therefore should be firstly screened during molecular diagnosis of CDAII. A c.325G>A mutation usually results in a mild to moderate clinical picture at homozygous level, whereas it may cause a very severe clinical pictures when combined with other mutations (Fermo, et al 2010). Despite the sequencing of all exons and flanking intronic regions, 5 patients displayed only one mutation, suggesting the possibility that mutations could be located in regulatory regions or that a second gene could be involved in the pathogenesis of CDAII. P. Bianchi and K. Schwarz contributed equally to this work. Disclosures: No relevant conflicts of interest to declare.
15
Wu, Jun, Fan Jiang, and Xiaofeng Lan. "Application of Superb Microvascular Imaging in the Diagnosis of Vascular Erectile Dysfunction." Journal of Medical Imaging and Health Informatics 11, no. 8 (August 1, 2021): 2248–52. http://dx.doi.org/10.1166/jmihi.2021.3771.
Повний текст джерелаАнотація:
To compare the difference between superb microvascular imaging (SMI) and color doppler flow imaging (CDFI) on vascular grading and blood flow display rate of the penile cavernous artery of vascular erectile dysfunction (ED), and to evaluate the length of time to obtain blood flow spectrum. 68 patients with ED were preformed for the examination of SMI and CDFI. The difference between the two techniques on vascular grading and blood flow display rate were compared. Hemodynamic parameters, namely peak systolic velocity (PSV), end diastolic velocity (EDV), and resistant index (RI) and the length of time obtained blood flow spectrum were compared. SMI was more sensitive to assess vascular grading and blood flow display rate than CDFI (P < 0.05). Hemodynamic parameters (PSV, EDV, and RI) measured by SMI and CDFI were well correlated (r = 0.981, P < 0.001; r = 0.879, P < 0.001; r = 0.937, P < 0.001). The duration of time necessary obtained the spectrums of grade 3 and grade 4 blood flow was shorter than that of grade 1 and grade 2, and SMI was comparatively shorter than CDFI (P < 0.05). In conclusion, SMI detected the blood flow of the penile cavernous artery better and maked the examination time shorter than CDFI, which can be considered as a more effective technique to diagnose vascular ED.
16
Jia, Yong-Yan, Xin-Nian Xie та Huai-Xia Yang. "Poly[[tetraaquabis(μ3-5-carboxybenzene-1,2,4-tricarboxylato)tricadmium] tetrahydrate]". Acta Crystallographica Section E Structure Reports Online 68, № 6 (26 травня 2012): m801—m802. http://dx.doi.org/10.1107/s1600536812022726.
Повний текст джерелаАнотація:
There are three independent CdII ions in the title complex, {[Cd3(C10H3O8)2(H2O)4]·4H2O} n , one of which is coordinated by four O atoms from three 5-carboxybenzene-1,2,4-tricarboxylate ligands and by two water molecules in a distorted octahedral geometry. The second CdII ion is coordinated by five O atoms from four 5-carboxybenzene-1,2,4-tricarboxylate ligands and by one water molecule also in a distorted octahedral geometry while the third CdII ion is coordinated by five O atoms from three 5-carboxybenzene-1,2,4-tricarboxylate ligands and by one water molecule in a highly distorted octahedral geometry. The 5-carboxybenzene-1,2,4-tricarboxylate ligands bridge the CdII ions, resulting in the formation of a three-dimensional structure. Intra- and intermolecular O—H...O hydrogen bonds are present throughout the three-dimensional structure.
17
Espelin, Christopher W., Kim T. Simons, Stephen C. Harrison, and Peter K. Sorger. "Binding of the EssentialSaccharomyces cerevisiaeKinetochore Protein Ndc10p to CDEII." Molecular Biology of the Cell 14, no. 11 (November 2003): 4557–68. http://dx.doi.org/10.1091/mbc.e02-08-0533.
Повний текст джерелаАнотація:
Chromosome segregation at mitosis depends critically on the accurate assembly of kinetochores and their stable attachment to microtubules. Analysis of Saccharomyces cerevisiae kinetochores has shown that they are complex structures containing ≥50 protein components. Many of these yeast proteins have orthologs in animal cells, suggesting that key aspects of kinetochore structure have been conserved through evolution, despite the remarkable differences between the 125-base pair centromeres of budding yeast and the Mb centromeres of animal cells. We describe here an analysis of S. cerevisiae Ndc10p, one of the four protein components of the CBF3 complex. CBF3 binds to the CDEIII element of centromeric DNA and initiates kinetochore assembly. Whereas CDEIII binding by Ndc10p requires the other components of CBF3, Ndc10p can bind on its own to CDEII, a region of centromeric DNA with no known binding partners. Ndc10p-CDEII binding involves a dispersed set of sequence-selective and -nonselective contacts over ∼80 base pairs of DNA, suggesting formation of a multimeric structure. CDEII-like sites, active in Ndc10p binding, are also present along chromosome arms. We propose that a polymeric Ndc10p complex formed on CDEII and CDEIII DNA is the foundation for recruiting microtubule attachment proteins to kinetochores. A similar type of polymeric structure on chromosome arms may mediate other chromosome–spindle interactions.
18
Assavajamroon, Ploy, Filip Kielar, Kittipong Chainok та Nanthawat Wannarit. "Crystal structure and photoluminescence properties of catena-poly[[bis(1-benzyl-1H-imidazole-κN 3)cadmium(II)]-di-μ-azido-κ4 N 1:N 3]". Acta Crystallographica Section E Crystallographic Communications 75, № 11 (29 жовтня 2019): 1748–52. http://dx.doi.org/10.1107/s205698901901421x.
Повний текст джерелаАнотація:
The new title one-dimensional CdII coordination polymer, [Cd(C10H10N2)2(μ1,3-N3)2] n , has been synthesized and structurally characterized by single-crystal X-ray diffraction. The asymmetric unit consists of a CdII ion, one azide and one 1-benzylimidazole (bzi) ligand. The CdII ion is located on an inversion centre and is surrounded in a distorted octahedral coordination sphere by six N atoms from four symmetry-related azide ligands and two symmetry-related bzi ligands. The CdII ions are linked by double azide bridging ligands within a μ1,3-N3 end-to-end (EE) coordination mode, leading to a one-dimensional linear structure extending parallel to [100]. The supramolecular framework is stabilized by the presence of weak C—H...N interactions, π–π stacking [centroid-to-centroid distance of 3.832 (2) Å] and C—H...π interactions between neighbouring chains.
19
Ma, Jian-Fang, Jin Yang, and Jing-Fu Liu. "A novel CdII coordination polymer with 1,1′-(1,4-butanediyl)diimidazole." Acta Crystallographica Section C Crystal Structure Communications 59, no. 11 (October 22, 2003): m482—m483. http://dx.doi.org/10.1107/s0108270103022443.
Повний текст джерелаАнотація:
The novel title CdII coordination polymer, poly[[dichlorocadmium(II)]-di-μ-1,1′-(1,4-butanediyl)diimidazole], [CdCl2(C10H14N4)2] n , (I), was obtained by reaction of CdCl2·2.5H2O and 1,1′-(1,4-butanediyl)diimidazole (hereafter L). In (I), each L molecule coordinates to two CdII cations through its two aromatic N atoms, thus acting as a bridging bidentate ligand. The CdII cations, which lie on the inversion centre, are bridged by four L molecules to form a two-dimensional (4,4)-network. The two-dimensional square-grid sheets are superimposed in an offset fashion.
20
Chen, Yun, Yu-Ling Wang, Shao-Ming Ying та Shuang-Lian Cai. "Poly[di-μ2-chlorido-μ4-hexamethylenetetramine-bis[chlorido(methanol-κO)cadmium(II)]]". Acta Crystallographica Section E Structure Reports Online 63, № 11 (17 жовтня 2007): m2751. http://dx.doi.org/10.1107/s1600536807046715.
Повний текст джерелаАнотація:
In the title complex, [Cd2Cl4(C6H12N4)(CH3OH)2] n , the hexamethylenetetramine (hmta) ligand is located on a twofold rotation axis and bridges four CdII ions through its four N atoms. Each CdII atom is coordinated, with a distorted octahedral geometry, by two N atoms from hmta ligands, two μ2-chloride anions, one terminal chloride anion and one methanol molecule. The μ2-chloride anions and hmta molecules link the CdII cations to form the three-dimensional polymeric structure. O—H...Cl hydrogen bonding between the methanol ligands and the terminal chloride anions is observed in the three-dimensional polymeric structure.
21
Farnia, Morteza F., Mansour Abedini, and Mohammad Ali Pazukian. "Preparation and Characterization of Complexes formed by the Reaction of Bis(4-hydroxyphenyl)-1,4-diazabuta-1,3-diene with Zinc, Cadmium and Mercury Chlorides." Journal of Chemical Research 23, no. 8 (August 1999): 494–95. http://dx.doi.org/10.1177/174751989902300820.
Повний текст джерелаАнотація:
Complexes of bis(4-hydroxyphenyl)-1,4-diazabuta-1,3-diene with ZnII, CdII and HgII chlorides are synthesized; the complexes of ZnII and CdII are mononuclear with the ligand as a chelate, whereas the HgII complex is dinuclear with the ligand bridging two Hg nuclei.
22
Xie, Zhimin, Junjie Fei, and Meihua Huang. "Differential Pulse Anodic Stripping Voltammetric Determination of Cadmium(II) at a Glassy Carbon Electrode Modified with a Nano-TiO2/Chitosan Composite Film." Australian Journal of Chemistry 61, no. 12 (2008): 1000. http://dx.doi.org/10.1071/ch08353.
Повний текст джерелаАнотація:
A simple and effective chemically modified electrode for the determination of cadmium(ii) was developed. The chemically modified electrode was prepared by modifying nano-TiO2/chitosan (nano-TiO2/CS) composite film onto a glassy carbon (GC) electrode. Compared with a bare GC electrode and a CS film-modified GC electrode, the nano-TiO2/CS composite film-coated GC electrode significantly improved the sensitivity of determining CdII. The effects of experimental parameters such as modifier composition in the GC electrode, pH of supporting electrolyte, accumulation potential and time were studied for analytical application. Under the optimized working conditions, the peak currents were linear with concentration over the range from 1.0 × 10–9 to 1.0 × 10–6 mol L–1 for CdII. The limit of detection is 2.0 × 10–10 mol L–1 CdII for 180-s accumulation. The application for the assay of trace levels of CdII in real water samples indicates that it is an economical and potent method.
23
Barcellini, Wilma, Anna Ines Gregorini, Giulia Soverini, Anna Zaninoni, Juri A. Giannotta, Cristina Vercellati, Valeria Ferri, Paola Bianchi, Agostino Cortelezzi, and Maria Domenica Cappellini. "Iron Overload and Cytokine Serum Levels in Congenital Hemolytic Anemias." Blood 128, no. 22 (December 2, 2016): 2458. http://dx.doi.org/10.1182/blood.v128.22.2458.2458.
Повний текст джерелаАнотація:
Abstract Background: Congenital hemolytic anemias (CHAs) are a heterogeneous group of inherited RBC disorders including membrane and enzyme defects and dyserythropoietic anemias. Iron overload is a well recognized complication of hereditary hemoglobinopathies, both in transfusion-dependent and independent cases. However, little is known in congenital hemolytic anemias, with the exception of anecdotic reports in pyruvate kinase deficiency and dyserythropoietic anemias. Aim: to describe the clinical and hematological features at diagnosis and enrolment, to investigate iron overload by hepatic and cardiac T2* MRI, and to study inflammatory/regulatory cytokine profiles (IL-6, TNF-alpha, IFN-gamma, IL-10, IL-17) and hepcidin levels in patients with CHAs. Confounding factors such as hemocromatosis genotyping, metabolic syndrome, and hepatic viral profile were also considered. Methods: Between July 2015 and April 2016, 38 patients were enrolled (13 hereditary spherocytosis -HS, 3 hereditary stomatocytosis - HSt, 8 congenital dyserythropoietic anemia type II - CDAII, 13 pyruvate kinase deficiency - PKD, 1 glucose-phosphate isomerase deficiency). HS cases were enrolled on the basis of ferritin >300 ng/mL at diagnosis. Cytokine levels were detected in serum by ELISA. Comparisons were made by Students T test (continuous) and Fisher's exact test (categorical), and correlations by Pearson's linear coefficient. Results: The main clinical and hematological findings are shown in table. Median Hb values progressively decreased in the 4 groups considered, being close to normal in HS and moderately reduced in CDAII patients, whereas hemolytic parameters were comparable among groups. Consistently with clinical severity, ferritin values were particularly high in CDAII (together with transferrin saturation-TfS) and PKD patients, notwithstanding chelation in about half cases of both groups. Of note, only 2 PKD patients were transfusion-dependent, suggesting that other factors are involved in iron overload. Splenectomy had been performed in 17/38 (44.7%), mainly CDAII. Liver iron concentration (LIC) showed a great heterogeneity in all groups, with a trend towards higher values in CDAII; 16/36 (44%) patients had a LIC>4 mg/g DW (23%, 33%, 38% and 88% in HS, HSt, PKD and CDAII, respectively). Cardiac T2* value was normal in all subjects, with the exception of a HS and a CDAII case. Regarding possible cofactors, 12/16 displayed at least one of the following: 1 homozygous for HFE C282Y and 1 for H63D mutations, 3 HCV+, 4 BMI>25, 2 alcohol abuse, 3 heterozygous for HFE mutations. The following positive correlations were observed at enrolment: LIC and ferritin (r=0.68, p<0.05), LIC and TfS (r=0.34, p=0.05), and cardiac T2* and TfS (r=0.34, p<0.05). Moreover Hb values at diagnosis negatively correlated with LIC (r=0.37, p<0.05). Interestingly, among the 28 cases with ferritin <800 ng/mL, 10 (36%) displayed liver iron overload (LIC>4), of whom 5 with the above listed cofactors. As regards cytokine levels, IL-10 was significantly increased in HS, PKD and CDAII groups compared with normal cases; TNF-alpha was decreased in HS and PKD, and IFN-gamma increased in HS and CDAII. Ferritin values were positively correlated with IL-6 and IFN-gamma, and TfS negatively with IL-6 (r= -0.38, p<0.05). Hepcidin values were slightly increased in CHAs compared with normal controls, and correlated positively with ferritin (r=0.33; p<0.05), and negatively with TfS (r= -0.56; p<0.001). Finally, hepcidin levels were positively correlated with IL-6 (r=0.62; p<0.001), and negatively with IFN-gamma (r=0.3; p<0.05). Conclusion: Iron overload is highly prevalent in CHAs, particularly in PKD and CDAII, is independent from transfusion support, and is also observed in cases with ferritin <800 ng/mL. T2* MRI is the gold standard approach to evaluate iron overload in CHAs (as in other congenital anemias) and its use is advisable, particularly in the presence of cofactors, for early chelation. Cytokine studies suggest the existence of a positive loop among ferritin, hepcidin, and inflammatory cytokines such as IL-6 and IFN-gamma, and of a negative loop among TfS, hepcidin, and the same inflammatory cytokines. These findings disclose important hints to understand the multiple biological mechanisms of iron overload, and support the rationale for new emerging therapies. Table Table. Disclosures Barcellini: Agios: Consultancy.
24
Usov, Pavel M., Tony D. Keene, and Deanna M. D'Alessandro. "A Comparative Study of the Structural, Optical, and Electrochemical Properties of Squarate-Based Coordination Frameworks." Australian Journal of Chemistry 66, no. 4 (2013): 429. http://dx.doi.org/10.1071/ch12474.
Повний текст джерелаАнотація:
Systematic studies of the thermal expansion, optical, and redox properties of a series of six squarate-based frameworks, [MII(C4O4)(H2O)2] (MII = MnII, FeII, CoII, NiII, ZnII, CdII) have revealed that five members of the series exhibit cubic structures in which the squarate ligands are configured in an ‘eclipsed’ phase, while the CdII analogue exhibits a trigonal structure with a ‘staggered’ orientation of the ligands. The ‘eclipsed’ structures are characterised by a positive coefficient of thermal expansion, while the CdII analogue exhibits zero thermal expansion. Ultraviolet-visible-near infrared (UV-Vis-NIR) spectra and electrochemical measurements indicate that electron delocalisation across the dianionic squarate bridge is absent.
25
Khoriaty, Rami, Angela Weyand, Geoffrey Hesketh, Amélie Bernard, Lesley Everett, Guojing Zhu, Beth McGee, et al. "Overlap of SEC23A and SEC23B Function Suggests a Novel Therapeutic Approach for Congenital Dyserythropoietic Anemia Type II." Blood 130, Suppl_1 (December 7, 2017): 80. http://dx.doi.org/10.1182/blood.v130.suppl_1.80.80.
Повний текст джерелаАнотація:
Abstract Congenital Dyserythropoietic Anemia type II (CDAII) is an autosomal recessive disease characterized by anemia and increased bone marrow (BM) bi/multi-nucleated erythroblasts. CDAII results from loss of function mutations in SEC23B encoding a core component of coat complex protein II (COPII) vesicles, which transport secretory proteins from the endoplasmic reticulum to the Golgi apparatus. Despite the identification of the genetic cause of CDAII, the pathophysiology of the disease remains unknown. Morpholino-induced SEC23B deficiency in zebrafish (ZF) has been previously reported to result in an erythroid phenotype mimicking CDAII (Shwartz et al, Nature genetics 2009), suggesting conservation of the underlying CDAII mechanism from fish to humans. Thus, we were puzzled to observe the absence of anemia or other CDAII characteristics in mice with erythroid specific (EpoR -Cre) and pan-hematopoietic (Vav1 -Cre) SEC23B deficiency (Khoriaty et al, MBC and Khoriaty et al, Sci Rep). To re-examine the ZF phenotype, we injected the morpholino targeting Sec23b into one-cell stage ZF embryos demonstrating no increase in circulating bi-nucleated erythroid cells, in contrast to the previous report. Given the variable knock-down that can result from morpholinos, we next generated ZF heterozygous for a 53 bp deletion (Sec23b+/-) using CRISPR/Cas9 genome editing. Intercrosses between Sec23b+/- ZF demonstrated lethality of Sec23b-/- ZF between days 17-21. However, the percentage of circulating bi-nucleated erythrocytes observed at day 16 was indistinguishable between Sec23b-/- ZF and wildtype (WT) clutch mate controls. Mammals and fish express two paralogs for SEC23, SEC23A and SEC23B, encoding highly related (~85%) proteins. To investigate the different functions of SEC23A and SEC23B, we defined the SEC23A and SEC23B interactomes using "BioID" (proximity dependent biotinylation) in HEK293 cells expressing BirA*-tagged SEC23A, SEC23B, or GFP control. Surprisingly, SEC23A and SEC23B exhibit indistinguishable interactomes. We also demonstrated that both mouse and human SEC23 paralogs can complement SEC23 deficiency in yeast. Similarly, rescue of the Sec23b-/- lethal phenotype in ZF by a Sec23a transgene demonstrated at least partial functional overlap of SEC23A/SEC23B function in vertebrates. To extend these observations to mammals, we genetically engineered the murine Sec23a cDNA into the endogenous mouse genomic locus of Sec23b . We demonstrated that SEC23B-deficient mice (previously shown to die perinatally from pancreatic degeneration) are rescued by SEC23A, exhibiting normal survival and pancreas histology, with no abnormalities apparent on detailed hematologic and anatomic examination. The expression of SEC23A and SEC23B mRNAs in human and mouse BMs were examined by qRT-PCR. SEC23B is the predominantly expressed paralog in human BM, with greater levels of SEC23A and reduced SEC23B in mouse BM. We therefore hypothesized that mice with erythroid deficiency of SEC23A alone or combined SEC23A/SEC23B deficiency might exhibit an erythroid defect. We first generated mice with erythroid-specific SEC23A deficiency, with the latter mice exhibiting no anemia or other CDAII characteristic. In contrast, mice with combined erythroid SEC23A and SEC23B deficiency die at ~E12.5, exhibiting reduced size and appear white in color compared to their WT litter mate controls, consistent with requirement of SEC23 in the erythroid compartment. Taken together, these data suggest complete (or near complete) overlap in function between SEC23A and SEC23B, and suggest that therapies that increase the expression of either SEC23 paralog might prove effective in treating CDAII. This paradigm might also apply to other disorders due to mutations in paralogous genes. Finally, our findings also suggest that a switch in paralog expression could account for other disparate disease phenotypes observed between animal models and humans. Disclosures No relevant conflicts of interest to declare.
26
Tan, Kemin, Parker M. Johnson, Lucy Stols, Bryan Boubion, William Eschenfeldt, Gyorgy Babnigg, Christopher S. Hayes, Andrezj Joachimiak, and Celia W. Goulding. "The structure of a contact-dependent growth-inhibition (CDI) immunity protein fromNeisseria meningitidisMC58." Acta Crystallographica Section F Structural Biology Communications 71, no. 6 (May 20, 2015): 702–9. http://dx.doi.org/10.1107/s2053230x15006585.
Повний текст джерелаАнотація:
Contact-dependent growth inhibition (CDI) is an important mechanism of intercellular competition between neighboring Gram-negative bacteria. CDI systems encode large surface-exposed CdiA effector proteins that carry a variety of C-terminal toxin domains (CdiA-CTs). All CDI+bacteria also produce CdiI immunity proteins that specifically bind to the cognate CdiA-CT and neutralize its toxin activity to prevent auto-inhibition. Here, the X-ray crystal structure of a CdiI immunity protein fromNeisseria meningitidisMC58 is presented at 1.45 Å resolution. The CdiI protein has structural homology to the Whirly family of RNA-binding proteins, but appears to lack the characteristic nucleic acid-binding motif of this family. Sequence homology suggests that the cognate CdiA-CT is related to the eukaryotic EndoU family of RNA-processing enzymes. A homology model is presented of the CdiA-CT based on the structure of the XendoU nuclease fromXenopus laevis. Molecular-docking simulations predict that the CdiA-CT toxin active site is occluded upon binding to the CdiI immunity protein. Together, these observations suggest that the immunity protein neutralizes toxin activity by preventing access to RNA substrates.
27
BARTOCCI, VITO, RITA GIOVANNETTI, and ENRICO CARSETTI. "Kinetics of the Metallation of Coproporphyrin-I in Water with Cadmium(II) and Manganese(II)." Journal of Porphyrins and Phthalocyanines 02, no. 02 (March 1998): 139–44. http://dx.doi.org/10.1002/(sici)1099-1409(199803/04)2:2<139::aid-jpp58>3.0.co;2-f.
Повний текст джерелаАнотація:
The reaction of 3,8,13,18-tetramethyl-21H,23H-porphine-2,7,12,17-tetrapropionic acid or coproporphyrin-I ( CPI ) with cadmium(II) was studied spectrophotometrically and its kinetic and equilibrium constants were determined. The influence of temperature on the reaction rate was also studied. At different ratios of [ CPI ]/[ Cd(II) ] two types of complex were formed: CdII ( CPI ) and ( CdII )2( CPI ); an investigation of the solution properties and the mechanism of aggregation of ( CdII )2( CPI ) at different pH were performed. It is verified that cadmium(II) accelerates the reaction of the incorporation of manganese(II) into CPI by the substitution of cadmium(II) with manganese(II) in the CdII ( CPI ) complex; the kinetics and mechanism of this substitution reaction at alkaline pH were studied. A sensitive kinetic method for the determination of cadmium(II) at ppb levels has been established; the molar absorptivity and the Sandell's sensitivity (for A = 0.001) of the recommended procedure at 458 nm and 300 s after the start of the reaction are (6.175 ± 0.026) × 105 1 mol−1 cm−1 and (1.820 ± 0.008) × 10−4 μg cm−2 respectively.
28
Powell, Kipton J., Paul L. Brown, Robert H. Byrne, Tamás Gajda, Glenn Hefter, Ann-Kathrin Leuz, Staffan Sjöberg, and Hans Wanner. "Chemical speciation of environmentally significant metals with inorganic ligands. Part 4: The Cd2+ + OH–, Cl–, CO32–, SO42–, and PO43– systems (IUPAC Technical Report)." Pure and Applied Chemistry 83, no. 5 (March 31, 2011): 1163–214. http://dx.doi.org/10.1351/pac-rep-10-08-09.
Повний текст джерелаАнотація:
The numerical modeling of CdII speciation amongst the environmental inorganic ligands Cl–, OH–, CO32–, SO42–, and PO43– requires reliable values for the relevant stability (formation) constants. This paper compiles and provides a critical review of these constants and related thermodynamic data. It recommends values of log10βp,q,r° valid at Im = 0 mol kg–1 and 25 °C (298.15 K), along with the equations and empirical reaction ion interaction coefficients, ∆ε , required to calculate log10βp,q,r values at higher ionic strengths using the Brønsted–Guggenheim–Scatchard specific ion interaction theory (SIT). Values for the corresponding reaction enthalpies, ∆rH, are reported where available. Unfortunately, with the exception of the CdII-chlorido system and (at low ionic strengths) the CdII-sulfato system, the equilibrium reactions for the title systems are relatively poorly characterized. In weakly acidic fresh water systems (–log10 {[H+]/c°} < 6), in the absence of organic ligands (e.g., humic substances), CdII speciation is dominated by Cd2+(aq), with CdSO4(aq) as a minor species. In this respect, CdII is similar to CuII [2007PBa] and PbII [2009PBa]. However, in weakly alkaline fresh water solutions, 7.5 < –log10 {[H+]/c°} < 8.6, the speciation of CdII is still dominated by Cd2+(aq), whereas for CuII [2007PBa] and PbII [2009PBa] the carbonato- species MCO3(aq) dominates. In weakly acidic saline systems (–log10 {[H+]/cϒ} < 6; –log10 {[Cl–]/c°} < 2.0) the speciation is dominated by CdCln(2–n)+ complexes, (n = 1–3), with Cd2+(aq) as a minor species. This is qualitatively similar to the situation for CuII and PbII. However, in weakly alkaline saline solutions, including seawater, the chlorido- complexes still dominate the speciation of CdII because of the relatively low stability of CdCO3(aq). In contrast, the speciation of CuII [2007PBa] and PbII [2009PBa] in seawater is dominated by the respective species MCO3(aq). There is scope for additional high-quality measurements in the Cd2+ + H+ + CO32– system as the large uncertainties in the stability constants for the Cd2+-carbonato complexes significantly affect the speciation calculations.
29
Jin, Yu-Xiu, Fang Yang, Li-Min Yuan, Chao-Guo Yan, and Wen-Long Liu. "A two-dimensional CdII coordination polymer with 2,2′-(disulfanediyl)dibenzoate and 1,10-phenanthroline ligands." Acta Crystallographica Section C Structural Chemistry 70, no. 5 (April 30, 2014): 517–21. http://dx.doi.org/10.1107/s2053229614009036.
Повний текст джерелаАнотація:
In poly[[μ3-2,2′-(disulfanediyl)dibenzoato-κ5 O:O,O′:O′′,O′′′](1,10-phenanthroline-κ2 N,N′)cadmium(II)], [Cd(C14H8O4S2)(C12H8N2)] n , the asymmetric unit contains one CdII cation, one 2,2′-(disulfanediyl)dibenzoate anion (denoted dtdb2−) and one 1,10-phenanthroline ligand (denoted phen). Each CdII centre is seven-coordinated by five O atoms of bridging/chelating carboxylate groups from three dtdb2− ligands and by two N atoms from one phen ligand, forming a distorted pentagonal–bipyramidal geometry. The CdII cations are bridged by dtdb2− anions to give a two-dimensional (4,4) layer. The layers are stacked to generate a three-dimensional supramolecular architecture via a combination of aromatic C—H...π and π–π interactions. The thermogravimetric and luminescence properties of this compound were also investigated.
30
Shirvan, Sadif A., та Sara Haydari Dezfuli. "catena-Poly[[(5,5′-dimethyl-2,2′-bipyridine-κ2 N,N′)cadmium]-di-μ-bromido]". Acta Crystallographica Section E Structure Reports Online 68, № 6 (31 травня 2012): m846. http://dx.doi.org/10.1107/s1600536812023860.
Повний текст джерелаАнотація:
In the crystal of the title polymeric compound, [CdBr2(C12H12N2)] n , the CdII cation is located on a twofold rotation axis. The CdII cation is six-coordinated in a distorted octahedral geometry formed by two N atoms from the 5,5′-dimethyl-2,2′-bipyridine ligand and four bridging Br− anions. The bridging function of the Br− anions leads to a polymeric chain running along the c axis.
31
Khoriaty, Rami, Matthew P. Vasievich, and David Ginsburg. "The COPII pathway and hematologic disease." Blood 120, no. 1 (July 5, 2012): 31–38. http://dx.doi.org/10.1182/blood-2012-01-292086.
Повний текст джерелаАнотація:
Abstract Multiple diseases, hematologic and nonhematologic, result from defects in the early secretory pathway. Congenital dyserythropoietic anemia type II (CDAII) and combined deficiency of coagulation factors V and VIII (F5F8D) are the 2 known hematologic diseases that result from defects in the endoplasmic reticulum (ER)–to–Golgi transport system. CDAII is caused by mutations in the SEC23B gene, which encodes a core component of the coat protein complex II (COPII). F5F8D results from mutations in either LMAN1 (lectin mannose-binding protein 1) or MCFD2 (multiple coagulation factor deficiency protein 2), which encode the ER cargo receptor complex LMAN1-MCFD2. These diseases and their molecular pathogenesis are the focus of this review.
32
Meng, Li, Miao-Li Zhu, and Li-Ping Lu. "Synthesis, crystal structure and characterization of a three-dimensional CdII coordination polymer constructed from 2,5-bis(1H-1,2,4-triazol-1-yl)terephthalate." Acta Crystallographica Section C Structural Chemistry 74, no. 2 (January 17, 2018): 166–70. http://dx.doi.org/10.1107/s2053229618000025.
Повний текст джерелаАнотація:
Bifunctional organic ligands are very popular for the design of coordination polymers because they allow the formation of a great diversity of structures. In the title coordination polymer, the new bifunctional inversion-symmetric ligand 2,5-bis(1H-1,2,4-triazol-1-yl)terephthalic acid (abbreviated as H2bttpa) links CdII cations, giving rise to the three-dimensional CdII coordination polymer catena-poly[diaqua[μ4-2,5-bis(1H-1,2,4-triazol-1-yl)terephthalato-κ4 O 1:O 4:N 4:N 4′]cadmium(II)], [Cd(C12H6N6O4)(H2O)2] n or [Cd(bttpa)(H2O)2] n . The asymmetric unit consists of half a CdII cation, half a bttpa2− ligand and one coordinated water molecule. The CdII cation is located on a twofold axis and is hexacoordinated in a distorted octahedral environment of four O and two N atoms. Four different bttpa2− ligands contribute to this coordination, with two carboxylate O atoms in trans positions and two triazole N atoms in cis positions. Two aqua ligands in cis positions complete the coordination sphere. The fully deprotonated bttpa2− ligand sits about a crystallographic centre of inversion and links two CdII cations to form a chain in a μ2-terephthalato-κ2 O 1:O 4 bridge. This chain extends in the other two directions via the triazole heterocycles, producing a three-dimensional framework. O—H...O hydrogen bonds and weak C—H...N interactions stabilize the three-dimensional crystal structure. The FT–IR spectrum, X-ray powder pattern, thermogravimetric behaviour and solid-state photoluminescence of the title polymer have been investigated. The photoluminescence is enhanced and red-shifted with respect to the uncoordinated ligand.
33
Truong, Khai-Nghi, Carina Merkens, and Ulli Englert. "3-(Pyridin-4-yl)acetylacetone: CdII and HgII compete for nitrogen coordination." Acta Crystallographica Section C Structural Chemistry 73, no. 9 (August 14, 2017): 724–30. http://dx.doi.org/10.1107/s2053229617011408.
Повний текст джерелаАнотація:
3-(Pyridin-4-yl)acetylacetone (HacacPy) acts as a pyridine-type ligand towards CdII and HgII halides. With CdBr2, the one-dimensional polymer [Cd(μ-Br)2(HacacPy)Cd(μ-Br)2(HacacPy)2]∞ is obtained in which five- and six-coordinated CdII cations alternate in the chain direction. Reaction of HacacPy with HgBr2 results in [Hg(μ-Br)Br(HacacPy)]∞, a polymer in which each HgII centre is tetracoordinated. In both compounds, each metal(II) cation is N-coordinated by at least one HacacPy ligand. Equimolar reaction between these CdII and HgII derivatives, either conducted in ethanol as solvent or via grinding in the solid state, leads to ligand redistribution and the formation of the well-ordered bimetallic polymer catena-poly[[bromidomercury(II)]-μ-bromido-[aquabis[4-hydroxy-3-(pyridin-4-yl)pent-3-en-2-one]cadmium(II)]-di-μ-bromido], [CdHgBr4(C10H11NO2)2(H2O)] n or [{HgBr}(μ-Br){(HacacPy)2Cd(H2O)}(μ-Br)2]∞. HgII and CdII cations alternate in the [100] direction. The HacacPy ligands do not bind to the HgII cations, which are tetracoordinated by three bridging and one terminal bromide ligand. The CdII centres adopt an only slightly distorted octahedral coordination. Three bromide ligands link them in a (2 + 1) pattern to neighbouring HgII atoms; two HacacPy ligands in a cis configuration, acting as N-atom donors, and a terminal aqua ligand complete the coordination sphere. Classical O—H...Br hydrogen bonds stabilize the polymeric chain. O—H...O hydrogen bonds between aqua H atoms and the uncoordinated carbonyl group of an HacacPy ligand in a neighbouring strand in the c direction link the chains into layers in the (010) plane.
34
Zhang, Dai, Ying Wang, Fan Yang, Yiran Mao, Jie Mu, Lihui Zhao, and Wengui Xu. "Diagnostic Value of Multi-Mode Ultrasonic Flow Imaging Examination in Solid Renal Tumors of Different Sizes." Journal of Clinical Medicine 12, no. 2 (January 10, 2023): 566. http://dx.doi.org/10.3390/jcm12020566.
Повний текст джерелаАнотація:
Purposes: To explore the value of Microflow Imaging (MFI) in renal solid tumors. Methods: A total of 195 patients with 199 lesions pathologically confirmed masses were retrospectively analyzed. The 199 masses were divided into the tumor ≤ 4 cm group (n = 104) and tumor > 4 cm group (n = 95). The diagnostic efficacy of Color Doppler Flow Imaging (CDFI), Power Doppler Imaging (PDI) and MFI in renal tumors sizes were compared by determining the Adler grade, vascular morphology and peripheral blood flow. Results: Among 199 tumors, 161 lesions were malignant and 38 lesions were benign. MFI in malignant tumor ≤ 4 cm demonstrated statistically significant differences in Adler grade and vascular morphology as compared to CDFI and PDI (p < 0.05). In malignant tumor > 4 cm group, MFI showed significant difference in vascular morphology compared with CDFI (p < 0.05). MFI showed a significant difference in the peripheral annular blood flow of malignant tumors when compared to CDFI and PDI (p < 0.05). In addition, the malignant tumors of the two sizes by MFI in peripheral annular blood flow detection showed significant difference (p < 0.05). The area under the curve of ROC by MFI in the tumor ≤ 4 cm was 0.771, which was higher than CDFI and PDI (p < 0.05), but no obvious difference among the tumor > 4 cm (p > 0.05). Conclusion: MFI provides a new method for the differential diagnosis of small renal carcinoma. Based on the convenience and non-radiation of MFI, we can choose MFI as an imaging diagnostic tool for patients who need long-term active surveillance (AS) follow-up.
35
Zhou, Qian-Kun, and Ni-Ya Li. "A three-dimensional cadmium coordination polymer based on 1,4-bis(1,2,4-triazol-1-yl)but-2-ene and benzene-1,3,5-tricarboxylic acid." Acta Crystallographica Section C Structural Chemistry 73, no. 9 (August 24, 2017): 749–53. http://dx.doi.org/10.1107/s2053229617012189.
Повний текст джерелаАнотація:
The CdII three-dimensional coordination poly[[[μ4-1,4-bis(1,2,4-triazol-1-yl)but-2-ene]bis(μ3-5-carboxybenzene-1,3-dicarboxylato)dicadmium(II)] dihydrate], {[Cd2(C9H4O6)2(C8H10N6)]·2H2O} n , (I), has been synthesized by the hydrothermal reaction of Cd(NO3)2·4H2O, benzene-1,3,5-tricarboxylic acid (1,3,5-H3BTC) and 1,4-bis(1,2,4-triazol-1-yl)but-2-ene (1,4-btbe). The IR spectrum suggests the presence of protonated carboxylic acid, deprotonated carboxylate and triazolyl groups. The purity of the bulk sample was confirmed by elemental analysis and X-ray powder diffraction. Single-crystal X-ray diffraction analysis reveals that the CdII ions adopt a five-coordinated distorted trigonal–bipyramidal geometry, coordinated by three O atoms from three different 1,3,5-HBTC2− ligands and two N atoms from two different 1,4-btbe ligands; the latter are situated on centres of inversion. The CdII centres are bridged by 1,3,5-HBTC2− and 1,4-btbe ligands into an overall three-dimensional framework. When the CdII centres and the tetradentate 1,4-btbe ligands are regarded as nodes, the three-dimensional topology can be simplified as a binodal 4,6-connected network. Thermogravimetric analysis confirms the presence of lattice water in (I). Photoluminescence studies imply that the emission of (I) may be ascribed to intraligand fluorescence.
36
Zhong, Kai-Long, Jing Quan, Xian-Xiao Pan, Wei Song, and Bing-Feng Li. "Synthesis, crystal structure and properties of a 2-D Cd(II) coordination polymer based on ferrocenecarboxylate and 4,4′-bipyridine ligands." Zeitschrift für Naturforschung B 77, no. 2-3 (December 16, 2021): 125–29. http://dx.doi.org/10.1515/znb-2021-0171.
Повний текст джерелаАнотація:
Abstract A new cadmium(II)-based coordination polymer [Cd3(FcCOO)6(4,4′-bipy)(H2O)2] n (FcCOO = ferrocenecarboxylato and 4,4′-bipy = 4,4′-bipyridine) has been synthesized under hydrothermal conditions and characterized by single-crystal X-ray diffraction. The results of a crystal structural analysis has revealed that the title compound consists of two crystallographically unique CdII centers, one in a general position with a five-coordinated and one on an inversion center with a six-coordinated environment. The CdII centers are connected by FcCOO− units to form a metal carboxylate oxygen chain extending parallel to the [100] direction while the 4,4′-bipy ligands further act as bridging linkers of the CdII centers resulting in a layered polymer. In addition, an X-ray powder diffraction and thermal gravimetric analysis and a cyclo-voltammetric characterization of the complex have also been carried out.
37
Pan, Tian-Tian, Jia-Geng Liu та Duan-Jun Xu. "Bis(4-hydroxybenzoato-κO)bis(1,10-phenanthroline-κ2 N,N′)cadmium(II) dihydrate". Acta Crystallographica Section E Structure Reports Online 62, № 7 (21 червня 2006): m1597—m1599. http://dx.doi.org/10.1107/s1600536806021787.
Повний текст джерелаАнотація:
In the title compound, [Cd(C7H5O3)2(C12H8N2)2]·2H2O, the CdII ion is located on a twofold axis and assumes a distorted octahedral CdN4O2 coordination geometry, formed by two phenanthroline (phen) ligands and two 4-hydroxybenzoate (HBA) anions. π–π stacking is observed between the parallel phen ligands of adjacent CdII complexes. One water O atom is located on a twofold axis. The other water molecule is disordered over two sites.
38
Li, Ni-Ya, and Dong Liu. "Synthesis, structure and photoluminescence properties of a three-dimensional cadmium(II) (4,5)-connected coordination network." Acta Crystallographica Section C Structural Chemistry 74, no. 12 (November 9, 2018): 1581–85. http://dx.doi.org/10.1107/s2053229618015073.
Повний текст джерелаАнотація:
The assembly of coordination polymers from metal ions and organic moieties is currently attracting considerable attention in crystal engineering due to their intriguing architectures and potential applications as functional materials. A new coordination polymer, namely poly[[μ2-trans-1,2-bis(pyridin-3-yl)ethylene-κ2 N:N′]bis(μ4-4,4′-oxydibenzoato-κ6 O:O,O′:O′′:O′′,O′′′)dicadmium(II)], [Cd2(C14H8O5)2(C12H10N2)] n or [Cd2(4,4′-OBB)2(3,3′-BPE)] n , has been synthesized by the the self-assembly of Cd(NO3)2·4H2O, 4,4′-oxydibenzoic acid (4,4′-H2OBB) and trans-1,2-bis(pyridin-3-yl)ethene (3,3′-BPE) under hydrothermal conditions. The title compound was structurally characterized by IR spectroscopy, elemental analysis and single-crystal X-ray diffraction analysis. Each CdII centre is coordinated by six carboxylate O atoms from four different 4,4′-OBB2− ligands and by one pyridyl N atom form a 3,3′-BPE ligand. Adjacent crystallographically equivalent CdII ions are bridged by 4,4′-OBB2− ligands, affording a two-dimensional [Cd(4,4′-OBB)] n net extending in the ac plane. Neighbouring [Cd(4,4′-OBB)] n nets are interlinked by 3,3′-BPE along the b axis to form a three-dimensional (3D) [Cd2(4,4′-OBB)2(3,3′-BPE)] n coordination network. In the network, each CdII centre is linked by four different 4,4′-OBB2− ligands and one 3,3′-BPE ligand. Meanwhile, each 4,4′-OBB2− ligand connects four separate CdII ions. Therefore, if the 4,4′-OBB2− ligands and CdII ions are considered as 4- and 5-connecting nodes, the structure of the title compound can be simplified as a 3D (4,5)-connected binodal framework with the rare (4462)(4466) TCS topology (Pearson, 1985; Blake et al., 2011). The thermal stability and photoluminescence properties of the title compound have also been investigated.
39
Bala, Madhu, and L. K. Mishra. "Complexing Behaviour and Antifungal Activity of N-[(1E)-1-(1H-Benzimidazol-2-yl)ethylidene]morpholine-4-carbothiohydrazide and Related Ligand with Metal Ions." International Journal of Inorganic Chemistry 2014 (April 22, 2014): 1–10. http://dx.doi.org/10.1155/2014/902575.
Повний текст джерелаАнотація:
The coordination complexes of bivalent metal ions with N-[(1E)-1-(1H-Benzimidazol-2-yl)ethylidene]morpholine-4-carbothiohydrazide (H2bmctz, H2L-1) and N-[(1E)-1-(1H-Benzimidazol-2-yl)(phenyl)methylidene] morpholine-4-carbothiohydrazide (H2bpmctz, H2L-2) were prepared and their neutral, monoanionic, and dianionic forms of ligands of compositions [M(H2L)X2] (M=CoII, NiII, CuII, ZnII, CdII, or PdII, X=Cl− or Br−, and H2L=H2L-1 or H2L-2), [M(HL)2]nH2O where (M=CoII, CuII, MnII, NiII, ZnII or CdII, H2L=H2L-1 or H2L-2, and n = 0 or 2), and [MLB]nB (M=CuII, NiII, ZnII, CdII, or PdII, B=H2O, Py, or Y-pic, n = 0 and n = 1 if B=H2O for Ni(II) and H2L=H2L-1 or H2L-2) have been characterised by magnetic susceptibility measurements, electrical conductance values, and spectral properties. The magnetic moment value of [M(HL2)] (M=MnII, NiII, FeII or CuII) type complexes is consistent with high spin octahedral structure while those of [M(H2L)X2] (M=CoII, NiII, CuII, ZnII, or CdII, X=Cl− or Br−) possess five coordinated trigonal bipyramidal geometry. The adduct complexes [MLB]·nB (M=NiII, or CuII, B=H2O, Py, or Y-pic) are four coordinated planar and those of ZnII and CdII complexes [MLB], (H2L=H2L-1 or H2L-2, B=H2O, Py or Y-pic) are tetrahedral. These ligands have been suggested to coordinate as tridentate (N N S) donor molecule in complexes of type [M(H2L)X2], [M(HL)2], and [MLB]. The antifungal activity of ligands and some of their metal complexes were studied and it was observed that metal complexes show higher activity than free ligand.
40
Khoriaty, Rami, Lesley Everett, Jennifer Chase, Guojing Zhu, Bin Zhang, Mark Hoenerhoff, Ivan Maillard, and David Ginsburg. "SEC23A Functionally Compensates for SEC23B Deficiency in Mice." Blood 126, no. 23 (December 3, 2015): 935. http://dx.doi.org/10.1182/blood.v126.23.935.935.
Повний текст джерелаАнотація:
Abstract Congenital Dyserythropoietic Anemia type II (CDAII) is a disease of ineffective erythropoiesis characterized by moderate anemia and increased bone marrow (BM) bi/multi-nucleated erythroid precursors. CDAII is an autosomal recessive disease resulting from mutations in SEC23B. SEC23 is a core component of COPII vesicles, which transport secretory proteins from the endoplasmic reticulum (ER) to the Golgi. Despite identification of the underlying genetic defect, the molecular mechanism by which SEC23B deficiency produces the unique CDAII phenotype remains unknown. We previously reported that mice homozygous for a Sec23b null allele die perinatally, exhibiting massive pancreatic degeneration, precluding evaluation of the adult erythroid compartment. To examine the impact of SEC23B deficiency on adult murine hematopoiesis, we generated mice with erythroid specific and pan-hematopoietic deficiency for SEC23B by crossing a second, conditional Sec23b allele (Sec23bfl), in which exons 5 and 6 are flanked by loxP sites, to an EpoR-Cre and Vav1-Cre, respectively. These mice did not exhibit anemia or any other CDAII characteristic. Similarly, mice transplanted with SEC23B-deficient fetal liver cells harvested from E17.5 embryos also failed to recapitulate any features of the CDAII phenotype. We next generated mice deficient in SEC23B exclusively in the pancreas by crossing the Sec23bfl allele to either p48-Cre or Pdx1-Cre. These mice exhibit a phenotype indistinguishable from mice with germline deletion of Sec23b, indicating that loss of pancreatic SEC23B is sufficient to explain the perinatal-lethality of global SEC23B deficiency in mice. The mammalian genome contains two paralogs for SEC23, SEC23A and SEC23B. These paralogs are highly identical at the amino acid level (~85%). We examined the relative expression of SEC23B/SEC23A in WT tissues from both humans and mice. This ratio is higher in human BM compared to pancreas, while it was higher in mouse pancreas compared to BM. In order to determine if SEC23A can rescue the phenotype of SEC23B deficient mice when expressed under the regulatory control of Sec23b, we genetically engineered the Sec23a cDNA into the endogenous genomic locus of Sec23b (Sec23a-b) in mouse embryonic stem cells via recombinase mediated cassette exchange. A heterozygous Sec23a-b intercross yielded the expected number of mice homozygous for the Sec23a-b allele. These mice exhibited normal survival, development, and fertility. Pancreas tissues dissected from Sec23a-b/a-b mice had normal weights, were histologically indistinguishable from WT controls, and did not exhibit dilated ER by transmission electron microscopy. Western blot analysis confirmed the absence of SEC23B in pancreata of Sec23a-b/a-b mice, with high levels of SEC23A expression. These data demonstrate that the SEC23A and SEC23B proteins overlap significantly (or completely) at the level of protein function, and suggest that the distinct phenotypes of human and mouse SEC23 deficiency are the result of an evolutionary shift in the tissue-specific gene expression programs of SEC23A and SEC23B. These findings also suggest that therapies that increase the expression of either SEC23 paralog in erythroid cells might be effective in CDAII. Disclosures No relevant conflicts of interest to declare.
41
Russo, Roberta, Immacolata Andolfo, Luigia De Falco, Francesco Manna, Antonella Gambale, Mariasole Bruno, Gianluca De Rosa, Domenico Girelli, Lucia De Franceschi, and Achille Iolascon. "Erfe-Encoding FAM132B in Congenital Dyserythropoietic Anemia Type II." Blood 126, no. 23 (December 3, 2015): 535. http://dx.doi.org/10.1182/blood.v126.23.535.535.
Повний текст джерелаАнотація:
Abstract Recessive mutations in SEC23B gene cause congenital dyserythropoietic anemia type II (CDAII), a rare hereditary disorder hallmarked by ineffective erythropoiesis, iron overload, and reduced expression of hepatic hormone hepcidin (Iolascon, 2013). The most recently described hepcidin regulator is the erythroblast-derived hormone erythroferrone (ERFE), a member of TNF-α superfamily that specifically inhibits hepcidin production in experimental models (Kautz, 2014). However, the function of ERFE in humans remains to be investigated. To determine whether dysregulation of ERFE expression is associated with ineffective erythropoiesis and iron-loading in CDAII, we studied the ERFE-encoding FAM132B gene expression in 48 SEC23B-related CDAII patients and 29 age and gender matched healthy controls (HCs). Twelve new cases and four novel SEC23B mutations were described. Samples were obtained after informed consent, according to the Declaration of Helsinki. Genomic DNA, mutational screening, RNA isolation, cDNA preparation, and qRT-PCR were performed as previously described (Russo, 2013). All patients were young adults (17.0±2.5 years at diagnosis), with increased serum ferritin (395.4±67.6 ng/mL) and transferrin saturation (71.9±5.4 %). We observed a statistically significant overexpression of FAM132B gene in peripheral blood mononuclear cells from CDAII patients (9.09±0.08) compared to HCs (8.32±0.12, p<0.0001). A similar trend was obtained when evaluating FAM132B expression in reticulocytes from a subset of patients and HCs. Of note, a statistically significant correlation between peripheral blood and reticulocyte FAM132B expression from the same patients was observed (Spearman ρ= 0.78, p=0.02). Although the role of ERFE in peripheral blood is still unknown, our observations suggested that the evaluation of FAM132B mRNA in peripheral blood is a reliable and easy-to-measure marker of ERFE levels. When we divided CDAII patients into two sub-groups accordingly to FAM132B gene expression, we observed a statistically significant reduction in hemoglobin (Hb) level in the high-FAM132B subset (8.6±0.4 g/dL) respect to low-FAM132B one (10.1±0.5 g/dL, p=0.02). Of note, the expression level of FAM132B did not correlate with the transfusion regimen. The higher amount of ERFE reflects the increased iron demand for Hb production as well as the expanding abnormal erythropoiesis, as attested by the increased RDW and sTfR (although not significant) in high-FAM132B patients. This in turn leads to reduced hepcidin in high-FAM132B group (4.2±1.8 nM) compared to low-FAM132B one (5.9±1.8 nM, p=0.05), resulting in augmented iron delivery to the erythron. Although the iron balance data do not differ significantly between the two groups, a tendency to decreased hepcidin/ferritin ratio and increased transferrin saturation was observed in high-FAM132B patients. Thus, FAM132B overexpression seems to contribute to the inappropriate suppression of hepcidin with subsequent hemosiderosis observed in CDAII. Consistent with our previous studies, we observed a reduced SEC23B expression in our patients compared to HC. Indeed, FAM132B and SEC23B gene expression exhibited an inverse correlation (Spearman ρ=-0.36, p=0.01). We confirmed the ex vivo data about inverse correlation between FAM132B and SEC23B expression observed in our patients by establishing K562 SEC23B-silenced cells. To knockdown SEC23B gene expression in K562 cells two different pGIPZ Lentiviral shRNAmir for SEC23B (shSEC23B-70/-74) were used. We observed a higher expression of FAM132B at 5 days of erythroid differentiation in K562 SEC23B-silenced cell compared to not-silenced ones. Conversely, SEC23B expression was lower in both shSEC23B compared to sh-CTR at 2 and 5 days of differentiation. Although the mechanisms of hemin-induced differentiation are quite different from EPO-induced ones, we can hypothesize that FAM132B over-expression is related to the maturative arrest and the subsequent increased number of erythroid precursors. This study provides the first analysis on ERFE regulation in humans. Our data suggest that ERFE over-expression in CDAII patients is the result of both physiological and pathological mechanisms leading to hepcidin suppression in condition of dyserythropoiesis. Nevertheless, it seems that ERFE cannot be the main erythroid regulator of hepcidin suppression, at least in CDAII patients. Disclosures No relevant conflicts of interest to declare.
42
Wallbank, Andrew I., Terry P. Lebold, Aneta Borecki, Alexander M. Polgar, Blake M. Waters, Mark S. Workentin, and John F. Corrigan. "ZnII and CdII Ferrocenechalcogenolate Complexes." European Journal of Inorganic Chemistry 2017, no. 2 (September 21, 2016): 372–77. http://dx.doi.org/10.1002/ejic.201600898.
Повний текст джерела
43
Gao, Shan, та Seik Weng Ng. "catena-Poly[[(tetraaquacadmium)-μ-4,4′-bipyridine-κ2 N:N′] 4-hydroxy-3-sulfonatobenzoate monohydrate]". Acta Crystallographica Section E Structure Reports Online 68, № 6 (2 травня 2012): m715. http://dx.doi.org/10.1107/s1600536812018727.
Повний текст джерелаАнотація:
The two independent CdII atoms in the polymeric title compound, [Cd(C10H8N2)(H2O)4](C7H4O6S)·H2O, lie on twofold rotation axes, and each is coordinated by four water molecules and the N atoms of two 4,4′-bipyridine molecules in an octahedral geometry. Bridging gives rise to chains along [101] and [-101]. The 4-hydroxy-3-sulfonatobenzoate dianions are not connected to the CdII atoms, but form hydrogen bonds to the coordinated water molecules as well as the lattice water molecule, generating a three-dimensional network.
44
Zhang, Hong-Kun, Xin Wang, Shuai Wang та Xiao-Dan Wang. "catena-Poly[[bis(nitrato-κO)cadmium]bis[μ-1,3-bis[(1H-1,2,4-triazol-1-yl)methyl]benzene-κ2 N 4:N 4′]]". Acta Crystallographica Section E Structure Reports Online 68, № 6 (31 травня 2012): m856. http://dx.doi.org/10.1107/s1600536812023288.
Повний текст джерелаАнотація:
In the title compound, [Cd(NO3)2(C12H12N6)2] n , the CdII cation is located on an inversion center and is six-coordinated by four N atoms from four 1,3-bis[(1H-1,2,4-triazol-1-yl)methyl]benzene (L) ligands and two O atoms from two nitrate anions in a slightly distorted octahedral geometry. The ligands link different CdII ions into a ribbon-like structure along [001]. Two O atoms of the nitrate anion are disordered over two sets of sites with site occupancies of 0.575 (8) and 0.425 (8).
45
Khoriaty, Rami, Matthew Vasievich, Morgan Jones, Jennifer Chase, Lesley Everett, Bin Zhang, Ivan Maillard, and David Ginsburg. "SEC23B Deficiency Results in Different Phenotypes in Humans and Mice." Blood 124, no. 21 (December 6, 2014): 1341. http://dx.doi.org/10.1182/blood.v124.21.1341.1341.
Повний текст джерелаАнотація:
Abstract SEC23B mutations in humans result in the autosomal recessive disease Congenital Dyserythropoietic Anemia type-II (CDAII). CDAII is characterized by moderate anemia in increased bone marrow (BM) bi/multi-nucleated erythroblasts. Despite the identification of the genetic defect underlying CDAII, the pathophysiology of this disease remains unknown. SEC23A and SEC23B are paralogous components of the coat protein complex II (COPII)-coated vesicles, which transport secretory proteins from the Endoplasmic Reticulum to the Golgi apparatus. We generated SEC23B-deficient mice and demonstrated that these animals die perinatally exhibiting massive pancreatic degeneration (Tao et. al PNAS). To examine the impact of SEC23B-deficiency on adult murine hematopoiesis, we harvested fetal liver cells (FLC), which contain hematopoietic stem cells, from SEC23B-deficient or wild-type (WT) control E17.5 embryos and transplanted them into lethally irradiated C57BL/6J mice. Recipients of SEC23B-deficient FLC did not exhibit anemia or any other CDAII characteristic (Khoriaty et. al, Mol Cell Biol), and SEC23B deficient FLC competed effectively with WT FLC at reconstituting hematopoiesis when transplanted into lethally irradiated recipient mice (Khoriaty et. al, Mol Cell Biol). A Sec23bfl conditional-allele was also generated. Mice with hematopoietic-specific SEC23B-deficiency, generated by crossing Vav1-Cre into Sec23bfl mice, also exhibited normal hematopoiesis. In contrast, mice with pancreas-specific Sec23b deficiency generated by crossing the Sec23bfl allele to a p48-Cre or Pdx1-Cre resulted in a phenotype indistinguishable from complete SEC23B-deficiency, demonstrating that loss of pancreatic Sec23b expression is sufficient to explain the perinatal lethality of SEC23B-deficient mice. To investigate different phenotypes of SEC23B deficiency in humans and mice, the SEC23B/SEC23A expression ratio was examined in murine and human tissues. This ratio is higher in mouse pancreas (12.7) compared to BM (2.6), whereas it is higher in human BM (7.8) relative to pancreas (5.5). Taken together with the high degree of amino-acid identity between SEC23A and SEC23B (~85%), these data suggest that the tissue-specific functions of SEC23A and SEC23B have shifted during evolution between humans and mice. To determine if Sec23a can rescue the lethality of SEC23B-deficient mice, we have engineered Sec23a cDNA into the endogenous genomic locus of Sec23b (Sec23A-B) via recombinase-mediated cassette exchange. A heterozygous Sec23A-B intercross is in progress. Rescue of the SEC23B deficient phenotype by SEC23A protein expressed under control of Sec23b regulatory sequences would suggest that increasing the expression of either paralog in erythroid cells might be effective in the treatment of CDAII. Disclosures No relevant conflicts of interest to declare.
46
Fermo, Elisa, Paola Bianchi, Cristina Vercellati, Wilma Barcellini, Carla Boschetti, Anna Paola Marcello, and Alberto Zanella. "Two Atypical Severe Cda Forms Presenting as Hydrops Foetalis Are Caused by Mutations in the SEC23B Gene." Blood 114, no. 22 (November 20, 2009): 1981. http://dx.doi.org/10.1182/blood.v114.22.1981.1981.
Повний текст джерелаАнотація:
Abstract Abstract 1981 Poster Board I-1003 Congenital dyserythropoietic anemias (CDAs) are a heterogeneous group of disorders characterized by ineffective erythropoiesis with prominent dysplastic features of the erythroid precursors in the bone marrow. Three main subtypes (I, II and III) have been identified, but several variants or atypical forms have been reported over the years. CDA are rarely associated with hydrops foetalis. In the past we described two cases presenting with hydrops foetalis and very severe anemia that were classified as atypical CDAs since they presented CDAII-like erythroblastic morphological features lacking other diagnostic CDAII markers. Very recently we and others (Bianchi et al, Human Mutat 2009, in press; Schwarz et al, Nat Genet 2009, in press) demonstrated that mutations in SEC23B gene, coding for a protein involved in the coat protein complex responsible for vesicle budding from the endoplasmic reticulum, cause CDA II. The aim of this work was to ascertain whether atypical CDAII-like forms presenting as hydrops foetalis could be caused by mutations in SEC23B gene and reclassified as CDAII. Two patients (Cantù-Rajnoldi et al, Br J Haematol 1997, 96: 530-3; Bianchi et al, Blood 1999, 94, suppl 1, 8b) from unrelated families with a history of intrauterine death of hydropic foetuses in previous pregnancies were referred at 20th week gestation following ultrasonic diagnosis of foetal hydrops and severe anemia (2.0 and 1.3 g/dL Hb respectively). In both cases intrauterine transfusions enabled the delivery of the babies. At birth both of them underwent extensive laboratory evaluation (including red cell metabolism and membrane proteins analysis) that was not informative on the causes of anemia. Ham test was repeatedly normal as for Western blotting analysis for GRP78 and glycoslylation pattern of red cell band 3. The bone marrow examination revealed the presence of 30 and 48% of bi- or multinucleated erythroblasts, some of whom presenting typical double outer membranes at transmission electron microscopy. Both babies became transfusion dependent. The 20 exons and intronic flanking regions of SEC23B gene were analyzed by direct sequencing. Both patients displayed mutations in SEC23B gene, in particular mutations c.325 G>A/ c.2101 C>T in patient 1 and c.325 G>A/ c.197G>A in patient 2 (Glu109Lys/ Arg701Cys and Glu109Lys/ Cys66Tyr respectively). c.325 G>A is the most frequent mutation so far described in CDAII and at homozygous level is usually associated with mild anemia. When found in compound heterozygosity with a second missense mutation as in these cases it may result in a very severe clinical pattern, although we cannot exclude that factors other than SEC23B mutations may contribute to worsening the clinical picture. In conclusion, SEC23B gene analysis allowed the correct classification of two very severe CDA cases associated with hydrops foetalis. This finding indicates that CDAII may result in intrauterine death and its frequency may therefore be underestimated. These cases may present as “atypical” for the lack of band3 hypoglycosylation likely due to the early sequestration of the more severely affected cells. Disclosures: No relevant conflicts of interest to declare.
47
Khoriaty, Rami, Matthew Vasievich, Morgan Jones, Bin Zhang, Lesley Everett, Jiayi Tao, Ivan Maillard, and David Ginsburg. "Disparate Phenotypes Of SEC23B Deficiency In Humans and Mice." Blood 122, no. 21 (November 15, 2013): 312. http://dx.doi.org/10.1182/blood.v122.21.312.312.
Повний текст джерелаАнотація:
Abstract Congenital dyserythropoietic anemia type-II (CDAII) is an autosomal recessive disease characterized by moderate anemia and increased bone marrow (BM) bi/multi-nucleated erythroblasts. CDAII results from mutations in SEC23B, one of two closely related mammalian SEC23 paralogs. SEC23 is a core component of COPII coated vesicles, which transport secretory proteins from the Endoplasmic Reticulum (ER) to the Golgi apparatus. Bone marrow transplantation cures CDAII, suggesting that the pathologic defect in this disease is restricted to the hematopoietic compartment. However, the mechanism by which SEC23B-deficiency results in CDAII remains unknown. We previously reported that mice homozygous deficient for SEC23B (Sec23bgt/gt) exhibit massive pancreatic degeneration. The latter resulted in perinatal mortality precluding evaluation of the adult hematopoietic compartment. To examine the impact of SEC23B-deficiency on adult murine hematopoiesis, fetal liver cells (FLC) were harvested from Sec23bgt/gt or wildtype (WT) control E17.5 embryos and transplanted into lethally irradiated C57BL/6J mice. Recipients of Sec23bgt/gt FLC had normal peripheral blood counts and were indistinguishable from recipients of WT FLC. To test for a more subtle hematopoietic defect, Sec23bgt/gt FLCs were tested directly against WT FLC for their ability to reconstitute hematopoiesis in a competitive repopulation assay. SEC23B deficient FLC exhibited no competitive disadvantage at reconstituting erythropoiesis relative to WT FLC over 18 weeks of follow-up. Transplant of marrow from these chimeric animals into secondary recipients demonstrated continued equivalence of Sec23bgt/gt and WT hematopoietic stem cells. We also generated a second, conditional Sec23b allele, in which exons 5 and 6 are flanked by loxP sites (Sec23bfl). Deletion of exons 5 and 6 with Cre-recombinase results in a frame shift leading to a stop codon in exon 7. Mice with erythroid-specific SEC23B deficiency were generated by crossing the Sec23bfl allele to an EpoR-Cre transgene. Sec23bfl/-/EpoR-CreTg+ mice maintained normal erythropoiesis indistinguishable from their WT littermates. Pancreas-specific knock-out generated by crossing the Sec23bfl allele to p48-Cre or Pdx1-Cre transgenes generated phenotypes indistinguishable from complete SEC23B deficiency, demonstrating that loss of pancreatic Sec23b expression alone is sufficient to explain the perinatal lethality observed in Sec23bgt/gt and Sec23b-/- mice. Our results conclusively demonstrate that in contrast to humans, SEC23B-deficiency results in massive pancreatic degeneration in mice, but no CDAII in these animals. To investigate the cause of the disparate human and mouse SEC23B-deficient phenotypes, SEC23B/SEC23A expression ratios were examined in endogenous tissues from both species. This ratio (normalized to the ratio in liver mRNA as 1.0) was higher in mouse pancreas (12.7) compared to BM (2.6), with the reverse pattern observed in human BM (7.8) relative to pancreas (5.5). These data, taken together with the high degree of identity between SEC23A and SEC23B (∼ 85% amino acid identity), suggest that the tissue-specific functions of SEC23A and SEC23B may have shifted during evolution between humans and mice. To test the role of SEC23A, we generated a mouse with a conditional Sec23a allele, in which exon 3 is flanked by loxP sites (Sec23afl). Cre-recombinase mediated deletion of exon 3 results in a frame shift leading to a stop codon in exon 7 (Sec23a-). Mice with erythroid-specific SEC23A-deficiency (Sec23afl/-/EpoR-CreTg+) maintained normal red blood cell counts indistinguishable from their WT littermates. In summary, we have shown that SEC23B-deficient humans and mice exhibit disparate phenotypes. We have also demonstrated variations in the gene expression programs for SEC23A and SEC23B potentially explaining the pancreatic phenotype of SEC23B-deficiency in mice and the erythroid phenotype in humans. These results suggest that the two SEC23 paralogs have overlapping functions and that therapeutic strategies that increase the expression of either SEC23A or SEC23B in erythroid cells might be effective in CDAII. Further studies of the overlapping functions of SEC23A and SEC23B and their relevant protein cargos should provide new insight into the pathogenesis of CDAII. Disclosures: No relevant conflicts of interest to declare.
48
Cané, Mariana. "La construcción discursiva de la inevitabilidad en los inicios del gobierno de la Alianza (Argentina, 1999-2000)." Papel Político 23, no. 2 (April 8, 2019): 1–23. http://dx.doi.org/10.11144/javeriana.papo23-2.cdii.
Повний текст джерелаАнотація:
Desde los inicios del gobierno de la Alianza (Diciembre 1999), gran parte de los discursos políticos establecieron polémicas en torno al significante “crisis”. En ese contexto, fueron enunciados una variedad de diagnósticos que disputaron por definir qué era lo que estaba en crisis, cuáles eran las causas de la misma y cómo debía ser conjurada. A partir del modo en que los discursos políticos delinearon los contornos de “la crisis”, hemos identificado tres grandes tópicas o conjuntos de argumentos (que denominamos fiscalista, asistencialista y mercadointernista) y un eje en disputa clave vinculado a la construcción de la inevitabilidad de las medidas a adoptar.
Aunque estas tópicas signaron el campo discursivo de lo político hasta fines del año 2001, se consolidaron con sus principales rasgos en el marco de la polémica por la reducción salarial y la aprobación de la reforma laboral de mediados del año 2000. En este sentido, sostenemos que es clave rastrear la construcción discursiva de la inevitabilidad porque constituye un elemento central para comprender parte del proceso de pérdida de legitimidad de la palabra política que alcanzó su punto de mayor algidez en lo que se conoció como “la crisis del 2001”.
49
Shao, Zhi-Chao, Xiang-Ru Meng, and Hong-Wei Hou. "Effect of pH on the construction of CdII coordination polymers involving the 1,1′-[1,4-phenylenebis(methylene)]bis(3,5-dicarboxylatopyridinium) ligand." Acta Crystallographica Section C Structural Chemistry 75, no. 8 (July 25, 2019): 1142–49. http://dx.doi.org/10.1107/s2053229619010192.
Повний текст джерелаАнотація:
Changing the pH value of a reaction system can result in polymers with very different compositions and architectures. Two new coordination polymers based on 1,1′-[1,4-phenylenebis(methylene)]bis(3,5-dicarboxylatopyridinium) (L 2−), namely catena-poly[[[tetraaquacadmium(II)]-μ2-1,1′-[1,4-phenylenebis(methylene)]bis(3,5-dicarboxylatopyridinium)] 1.66-hydrate], {[Cd(C22H14N2O8)(H2O)4]·1.66H2O} n , (I), and poly[{μ6-1,1′-[1,4-phenylenebis(methylene)]bis(3,5-dicarboxylatopyridinium)}cadmium(II)], [Cd(C22H14N2O8)] n , (II), have been prepared in the presence of NaOH or HNO3 and structurally characterized by single-crystal X-ray diffraction. In polymer (I), each CdII ion is coordinated by two halves of independent L 2− ligands, forming a one-dimensional chain structure. In the crystal, these chains are further connected through O—H...O hydrogen bonds, leading to a three-dimensional hydrogen-bonded network. In polymer (II), each hexadentate L 2− ligand coordinates to six CdII ions, resulting in a three-dimensional network structure, in which all of the CdII ions and L 2− ligands are equivalent, respectively. The IR spectra, thermogravimetric analyses and fluorescence properties of both reported compounds were investigated.
50
Chen, Ming, Xiaohong Fu, and Yan Shen. "Evaluation of Multimode Color Doppler Flow Imaging in the Diagnosis of Solid Renal Tumor." Contrast Media & Molecular Imaging 2021 (April 1, 2021): 1–7. http://dx.doi.org/10.1155/2021/6656877.
Повний текст джерелаАнотація:
Background. Renal cell carcinoma is one of the most common malignant tumors in urinary system, seriously affecting people’s health and life. This study aimed to evaluate the clinical value of multi-mode color Doppler flow imaging for diagnosis of solid renal tumor. Methods. Sixty-six renal solid tumors from 63 patients were examined by color Doppler flow imaging (CDFI), power Doppler flow imaging (PDFI), superb microvascular imaging (SMI), and contrast-enhanced ultrasound (CEUS) before surgery. The diagnostic efficacy of the four methods was compared by determining blood flow grade and ring-like blood flow with Adler’s method. Chi-square test and Fisher’s test were performed to compare the results of sensitivity and specificity among four methods. Results. Statistically significant differences in blood flow grade and ring-like blood flow were observed between benign and malignant renal tumors as detected by SMI and CEUS ( P < 0.05), whereas no difference was found as detected by CDFI and PDFI ( P > 0.05). The results indicated that the sensitivity and specificity of SMI (82.46%, 88.89%) and CEUS (84.21%, 88.89%) were higher than those of CDFI (42.11%, 66.67%) and PDFI (47.37%, 77.78%). Compared with the abilities of CDFI and PDFI, SMI and CEUS can better display the micro-blood flow in the tumors and evaluate the blood flow grading, which indicated that SMI and CEUS may have high values in the differential diagnosis of benign and malignant solid renal tumors. Conclusion. SMI and CEUS can improve the sensitivity and specificity of the diagnosis of benign and malignant renal tumors and have a high application value.