Добірка наукової літератури з теми "CD34+/CD45+"
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Статті в журналах з теми "CD34+/CD45+"
Ogata, Kiyoyuki, Chikako Satoh, Mikiko Tachibana, Hideya Hyodo, Hideto Tamura, Kazuo Dan, Takafumi Kimura, Yoshiaki Sonoda, and Takashi Tsuji. "Identification and Hematopoietic Potential of CD45-Negative Clonal Cells with Very Immature Phenotype (CD45−CD34−CD38−Lin−) in Patients with Myelodysplastic Syndromes." Blood 104, no. 11 (November 16, 2004): 3426. http://dx.doi.org/10.1182/blood.v104.11.3426.3426.
Повний текст джерелаLange, Andrzej, Dorota Dlubek, Barbara Wysoczanska, Daria Drabczak-Skrzypek, and Emilia Jaskula. "An Evidence That Mesenchymal Stem Cells Are Not Replaced by Peripheral Blood Stem Cell Allografts in CML Patients." Blood 106, no. 11 (November 16, 2005): 4875. http://dx.doi.org/10.1182/blood.v106.11.4875.4875.
Повний текст джерелаTimmermans, Frank, Magda De Smedt, Robrecht Raedt, Jean Plum, and Bart Vandekerckhove. "Endothelial Cells Are Not Derived from Hematopoietic Precursor Cells." Blood 108, no. 11 (November 16, 2006): 1815. http://dx.doi.org/10.1182/blood.v108.11.1815.1815.
Повний текст джерелаDao, Mo A., Jesusa Arevalo, and Jan A. Nolta. "Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution." Blood 101, no. 1 (January 1, 2003): 112–18. http://dx.doi.org/10.1182/blood-2002-01-0025.
Повний текст джерелаMancuso, Patrizia, Ines Martin Padura, Giuliana Gregato, Paola Marighetti, Angelica Calleri, Chiara Corsini, Giancarlo Pruneri, Visnu Lohsiriwat, Jean Yves Petit, and Francesco Bertolini. "CD45-CD34+ Endothelial Progenitor Cells (EPCs) from Human Adipose Tissue Promote Tumor Growth and Metastases." Blood 118, no. 21 (November 18, 2011): 2208. http://dx.doi.org/10.1182/blood.v118.21.2208.2208.
Повний текст джерелаFritsch, G., P. Buchinger, D. Printz, FM Fink, G. Mann, C. Peters, T. Wagner, A. Adler, and H. Gadner. "Rapid discrimination of early CD34+ myeloid progenitors using CD45-RA analysis." Blood 81, no. 9 (May 1, 1993): 2301–9. http://dx.doi.org/10.1182/blood.v81.9.2301.2301.
Повний текст джерелаFritsch, G., P. Buchinger, D. Printz, FM Fink, G. Mann, C. Peters, T. Wagner, A. Adler, and H. Gadner. "Rapid discrimination of early CD34+ myeloid progenitors using CD45-RA analysis." Blood 81, no. 9 (May 1, 1993): 2301–9. http://dx.doi.org/10.1182/blood.v81.9.2301.bloodjournal8192301.
Повний текст джерелаVodyanik, Maxim A., James A. Thomson, and Igor I. Slukvin. "Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures." Blood 108, no. 6 (September 15, 2006): 2095–105. http://dx.doi.org/10.1182/blood-2006-02-003327.
Повний текст джерелаCiraci, Elisa, Silvia Della Bella, Ombretta Salvucci, Cristina Rofani, Marta Segarra, Caterina Bason, Agnese Molinari, Dragan Maric, Giovanna Tosato, and Anna C. Berardi. "Adult human circulating CD34−Lin−CD45−CD133− cells can differentiate into hematopoietic and endothelial cells." Blood 118, no. 8 (August 25, 2011): 2105–15. http://dx.doi.org/10.1182/blood-2010-10-316596.
Повний текст джерелаCase, Jamie, Laura E. Mead, Hilary A. White, Mohammad R. Saadatzadeh, Mervin C. Yoder, Laura S. Haneline, and David A. Ingram. "CD34+AC133+VEGFR-2+ Cells Are Primitive Hematopoietic Progenitors, Not Functional Endothelial Progenitor Cells." Blood 108, no. 11 (November 16, 2006): 1796. http://dx.doi.org/10.1182/blood.v108.11.1796.1796.
Повний текст джерелаДисертації з теми "CD34+/CD45+"
Rocha, Francielle Ramalho. "Desenvolvimento e validação de controle de qualidade interno in house para quantificação de células progenitoras hematopoéticas CD34+/CD45+." Botucatu, 2020. http://hdl.handle.net/11449/192307.
Повний текст джерелаResumo: O sistema de qualidade é de suma importância em laboratórios clínicos para avaliação de processos analíticos de maneira que os resultados liberados sejam verdadeiros. Para a metodologia de imunofenotipagem celular por citometria de fluxo as amostras devem ser frescas e os exames realizados preferencialmente dentro de 48 horas. É relevante utilizar amostras de controle de qualidade internos (CQI) padronizadas, de modo que possam ser repetidas rotineiramente, como referencial de qualidade. No Brasil, poucos serviços comercializam amostras preservadas para uso como CQI. Deste modo, a padronização in house com validação de processo para obtenção de amostras que possam ser utilizadas para esta finalidade é relevante. O objetivo deste trabalho foi desenvolver controle de qualidade interno para as rotinas de quantificação de células progenitoras hematopoéticas (CPH), utilizando solução preservante e avaliar a reprodutibilidade e estabilidade ao longo do tempo. Foram preparadas soluções preservantes contendo diferentes concentrações de anticoagulantes e fixadores, e destas, foi selecionada uma composição, originalmente padronizada neste estudo. Foram utilizados 5mL de sangue periférico, sendo este acrescido da solução a ser testada. Imediatamente, realizou-se a quantificação das populações de CPH em tubo Trucount®, usando anti-CD45, anti-CD34 e 7-AAD, conforme indicado pelo fabricante. A leitura foi realizada em citômetro de fluxo modelo FACSCalibur®-BD, para obtenção dos valores abs... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The quality system is of paramount importance in clinical laboratories for evaluating analytical processes in order to consider true the released results. The samples must be performed fresh preferably within 48 hours for the cell immunophenotyping methodology by flow cytometry. It is relevant to use standardized internal quality control (IQC) samples, thus they could be repeated routinely, as a quality benchmark. In Brazil, only a few services commercialize preserved samples for use as IQC. Therefore, it is relevant to use in-house standardization with process validation to obtain samples that can be used for this purpose. The objective of this work was to develop an IQC for a daily routine quantification of hematopoietic stem cells (HSCs) by using a preservative solution and to evaluate the reproducibility and stability over time. Preservative solutions containing different concentrations of anticoagulants and fixatives were prepared, and from these, a composition was selected, which was previously originally standardized in this study. 5mL of peripheral blood were used, which was added to the solution to be tested. The HSCs populations were immediately quantified in a Trucount® tube, using anti-CD45, anti-CD34 and 7-AAD, as indicated by the manufacturer. The reading was performed in a flow cytometer model FACSCalibur®-BD in order to obtain the absolute values of HSCs on day zero, 7, 21, 35 and 49. During this period, the samples were kept refrigerated (2 to 8ºC). The value... (Complete abstract click electronic access below)
Mestre
Guimarães, Tânia Maria Rocha 1963. "Perfil de expressão de células progenitoras endoteliais circulantes CD45-/ CD34+/KDR+ em mulheres hipertensas na pré-menopausa em comparação com mulheres saudáveis normotensas = Expression profile of circulating endothelial progenitor cells CD45-/ CD34+/KDR+ in hypertensive premenopausal women compared with healthy normotensive women." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317311.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
Made available in DSpace on 2018-08-26T00:13:01Z (GMT). No. of bitstreams: 1 Guimaraes_TaniaMariaRocha_D.pdf: 3499853 bytes, checksum: 2767ae0d1bab61dda96ce4765cd63588 (MD5) Previous issue date: 2014
Resumo: As células progenitoras endoteliais (EPCs) estão envolvidas em neovasculogênese e na manutenção da homeostase vascular, sua deficiência pode ter papel na patogênese da hipertensão. Este estudo teve como objetivo analisar o perfil de expressão das EPCs circulantes e diferentes fatores de risco cardiovascular em mulheres hipertensas, na pré-menopausa, em comparação com mulheres normotensas saudáveis. Realizou-se um estudo caso-controle com 45 mulheres voluntárias, faixa etária de 30 a 50 anos (41 ± 6) no Ambulatório do Pronto Socorro Cardiológico de Pernambuco. As EPCs definidas como CD45-/CD34+/KDR+ foram coletadas em sangue venoso periférico e analisadas por citometria de fluxo. As mulheres foram classificadas como controles (CT) saudáveis normotensas com PAS (pressão arterial sistólica) < 130 mmHg e PAD (pressão arterial diastólica) < 85 mmHg (n=15), com hipertensão primária: a) Leve (HL) PAS=140-159mm Hg e PAD=90-99 mmHg (n=15) e b) Severa (HS) PAS > 180 mmHg e PAD > 110 mmHg (n=15). Os grupos foram entrevistados quanto aos hábitos de fumo, prática de exercícios físicos e Índice de Massa Corporal (IMC), sendo aferido o nível da PA em repouso. Realizou-se análise nos prontuários dos resultados dos exames séricos de colesterol total, lipoproteínas de alta densidade-colesterol (HDL-c), lipoproteínas de baixa densidade-colesterol (LDL-c), triglicerídeos e glicemia de jejum, no mês da coleta das amostras sanguíneas. Os resultados comprovaram redução significativa ao número de EPCs no HL (74%) e HS (88%) versus CT; e redução de 67% no HS versus HL, evidenciando relação inversa entre o número de células e o estágio da hipertensão. O grupo HS apresentou aumento de 49% de células CD45+ demonstrando padrão inflamatório e redução de 61% de CD45-/CD34+. Quanto aos níveis séricos verificou-se: HDL-c [HL (52±7); HS (48±5)]; LDL-c [HL (130±8); HS (143±15)]; triglicerídeos [HL (138±19); HS(153 ±40)]; glicemia de jejum [HL(95±7);HS(121±39)] e IMC [HL(31±4);HS(29±3)]; revelando que 67% das mulheres com hipertensão severa apresentavam síndrome metabólica (SM). O desenvolvimento da hipertensão e da SM foi diretamente correlacionado com a diminuição das EPCs. Portanto, a contagem de EPCs pode ser considerada um marcador biológico adequado para indicar a gravidade do estado hipertensivo em mulheres
Abstract: Endothelial progenitor cells (EPCs) are involved in neovasculogenesis and maintenance of vascular homeostasis and their impairment may have a role in the pathogenesis of hypertension. This study aimed analyzes the expression profile of circulating EPCs and different cardiovascular risk factors in hypertensive premenopausal women compared with healthy normotensive women. A case-control study was conducted enrolling 45 women volunteers, aged from 30- 50 years (41 ± 6) in Ambulatory of the Cardiologic Emergency Hospital of Pernambuco. EPCs numbers were determined by flow cytometry in peripheral blood as the CD45-/CD34+/KDR+ cells. The women were classified as healthy normotensive controls (CT) with SBP (systolic blood pressure) <130 mmHg and DBP (diastolic blood pressure) < 85 mmHg (n=15), and with essential hypertension; a) mild (MH), SBP=140-159 mmHg and DBP=90-99 mmHg (n=15); and b) severe (SH), SBP>180 mmHg and DBP>110 mmHg (n=15). The group were interviewed regarding smoking habits, physical exercise and body mass index (BMI), and measured the level of blood pressure at quiescent. An analysis in records of test results cholesterol, high density lipoprotein-cholesterol (HDL-c), low density lipoprotein-cholesterol (LDL-c), triglycerides and fasting glucose in the month of collection of blood samples. The results found a significant reduction in circulating EPCs numbers in MH (74%) and SH (88%) when compared to the CT and reduction of 67% in SH when compared to MH, an inverse relationship between the number of cells and the stage of hypertension. SH group showed an increase of 49% CD45+ cells demonstrating inflammation and reduction of 61% CD45-/ CD34+ cells. Regarding the biochemical serum was found: HDL-c [MH (52±7); SH (48±5)]; LDL-c [MH (130±8); SH (143±15)]; triglycerides [MH (138±19); SH (153±40)]; fasting glucose [MH (95±7); SH (121±39)] and BMI [MH (31±4); SH (29±3)]; revealing that 67% of women with severe hypertension had metabolic syndrome (MS). Development of hypertension and the parameters related to MS are directly correlated with a decrease of circulating EPCs. Therefore, the EPCs counting may be considered a suitable biological marker to follow up the evolution of the hypertensive state in women
Doutorado
Biologia Celular
Doutora em Biologia Celular e Estrutural
Golbs, Sebastian. "Entzündungsparameter und Vorläufermarker bei der Coronaratherosklerose." Doctoral thesis, Universitätsbibliothek Leipzig, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:15-20100407-135735-3.
Повний текст джерелаZaidan, Nada Mousa O. "The role of Gata3 in blood stem cell emergence." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274544.
Повний текст джерелаGolbs, Sebastian. "Entzündungsparameter und Vorläufermarker bei der Coronaratherosklerose." Doctoral thesis, 2009. https://ul.qucosa.de/id/qucosa%3A10826.
Повний текст джерелаЧастини книг з теми "CD34+/CD45+"
Park, Tea Soon, Paul W. Burridge, and Elias T. Zambidis. "Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells." In Springer Protocols Handbooks, 337–50. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-267-0_24.
Повний текст джерелаТези доповідей конференцій з теми "CD34+/CD45+"
Mukherjee, Kalyan K., Debasish Banerjee, Anjan Das, Subham Halder, Dattatreya Mukherjee, Shyam S. Mondal, Surya K. Roy, Mili Das, Chinmay K. Panda, and Utpal Chaudhuri. "Significance of Detecting Minimal Residual Disease by Flow Cytometry and its Impact on Overall Survival and Prognosis of Pediatric B-Cell ALL Patient Experience from a Tertiary Care Centre in Eastern India." In Annual Conference of Indian Society of Medical and Paediatric Oncology (ISMPO). Thieme Medical and Scientific Publishers Pvt. Ltd., 2021. http://dx.doi.org/10.1055/s-0041-1735366.
Повний текст джерелаBenton, Christopher B., Ahmed Al Rawi, Taejin Min, Rui-Yu Wang, Wendy Schober, Zhiqiang Wang, Zhihong Zeng, et al. "Abstract 1391: Single cell analysis of Lin-CD34-CD45- cells from primary AML samples reveals leukemia clones with stem cell-like properties distinct from CD34+CD38-CD123+ LSC." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-1391.
Повний текст джерелаАпарцин, Константин, and Konstantin Apartsin. "The results of fundamental and translational research carried out In the Department of Biomedical Research and Technology of the SBRAS INC in 2012-2016." In Topical issues of translational medicine: a collection of articles dedicated to the 5th anniversary of the day The creation of a department for biomedical research and technology of the Irkutsk Scientific Center Siberian Branch of RAS. Москва: INFRA-M Academic Publishing LLC., 2017. http://dx.doi.org/10.12737/conferencearticle_58be81eca22ad.
Повний текст джерела