Дисертації з теми "Cataboliti"
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Tongyoo, Narongchai. "Physical and functional analysis of genes from the cam catabolic plasmid encoding probable steps in the catabolism of camphor." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397983.
Повний текст джерелаChou, Han Ting. "L-Lysine Decarboxylase and Cadaverine Gamma-Glutamylation Pathways in Pseudomonas Aeruginosa PAO1." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/biology_diss/103.
Повний текст джерелаMadhushani, W. K. Anjana. "Multiple regulatory inputs for hierarchical control of phenol catabolism by Pseudomonas putida." Doctoral thesis, Umeå universitet, Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106878.
Повний текст джерелаDantas, Hugo Miguel Campelo. "Engineering hexuronic acid catabolism." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/11776.
Повний текст джерелаA engenharia metabólica é uma área emergente que visa o aperfeiçoamento de vias metabólicas para produção de compostos valiosos. A produção mundial de casca de frutos cítricos é estimada em 15,000,000 de toneladas por ano, e o seu descarte causa problemas ambientais. O principal constituinte da casca de frutos cítricos é o ácido D-galacturónico. O objetivo deste projeto é converter o ácido D-galacturónico noutros químicos proveitosos, utilizando para tal bolores geneticamente modificados. Aspergillus niger foi escolhido por ser naturalmente um bom consumidor do ácido Dgalacturónico e produtor das enzimas necessárias à hidrólise de casca de frutos cítricos. No presente trabalho, estirpes de Aspergillus niger foram geneticamente modificados onde (i) o gene gaaB que codifica para a L-galactonato desidratase foi deletado (ΔgaaB) e (ii) o gene gaaB foi deletado e o gene gaaA que codifica para a D-galacturonato reductase se encontrava sobreexpresso (ΔgaaB-gaaA). Estas estirpes foram utilizadas para fermentação submersa e em estado sólido para converter casca de laranja em L-galactonato num processo consolidado. As estirpes foram capazes de converter, até 87 %, de ácido D-galacturónico em L-galactonato por fermentação em estado sólido. Outra via metabólica estudada foi a via eucariota do ácido glucurónico. Nesta via metabólica é uma descarboxilase que converte o 3-ceto-L-gulonato em Lxilulose. A reação ainda não está claramente caracterizada e o gene não é conhecido. Um teste enzimático acoplado foi realizado de forma a testar a sua atividade. Neste ensaio o ácido L-gulónico é o substrato inicial, uma Lgulonato- 3-desidrogenase NAD-dependente (GDH) que produz o substrato para a descarboxilase. A L-xilulose reductase é então detetada por uma Lxilulose reductase NADPH-dependente de Aspergillus niger (lxrA). Para seguir a reação, o NADPH foi monitorizado a 340 nm. Para evitar a interferência do NADH que também absorve a 340 nm, Tio-NAD+ foi usado para a desidrogenase. GDH e lxrA ativas foram preparadas e o ensaio testado com precipitados sulfato de amónio de extrato de fígado bovino. A atividade da 3-ceto-Lgulonato descarboxilase não foi detetada.
Stankiewicz, Margaret J. "Oxidative catabolism of tetrahydropterins." Thesis, Aston University, 1989. http://publications.aston.ac.uk/12531/.
Повний текст джерелаCrabbe, T. B. "Studies on the adenylate cyclase and HMGCoA reductase of the yeast Saccharomyces cerevisiae." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233812.
Повний текст джерелаBirch, D. J. "Carbon catabolite repression in the yeast Saccharomyces cerevisiae." Thesis, University of Liverpool, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372682.
Повний текст джерелаJones, Melissa Kaye. "Inositol catabolism in Drosophila melanogaster." Thesis, California State University, Long Beach, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1527384.
Повний текст джерелаmyo-Inositoloxygenase (MIOX) catalyzes the first step in myo-inositol catabolism. MIOX has not been annotated in Drosophila melanogaster, but the protein encoded by the CG6910 gene is similar to the mouse MIOX protein. CG6910 "knocked-down" expression was explored using RNAi. "Knock-down" flies did not survive on inositol defined media, indicating that CG6910 encodes MIOX. Survival of these flies on sucrose defined media suggest that MIOX is not essential for development. Biochemical assays demonstrated that D. melanogaster has MIOX activity. Computational analyses revealed potential miRNA sites, and that a number of essential components are conserved. MIOX genes found in other drosopholids are highly similar to D. melanogaster MIOX, and analyses of the syntenic regions concur with established evolution. Western blot analyses showed differential expression amongst D. melanogaster from different geographic locations and between species. These studies may contribute to understanding the role of inositol catabolism in fruit fly development and diabetes.
Brummett, Adam Eugene. "Enzymology of microbial dimethylsulfoniopropionate catabolism." Diss., University of Iowa, 2017. https://ir.uiowa.edu/etd/5430.
Повний текст джерелаKandasamy, Dineshkumar. "Study on yeast enzymes Urc1p and Urc4p in a novel uracil catabolism pathway (URC)." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-185013.
Повний текст джерелаGrimsley, Philip George Medical Sciences Faculty of Medicine UNSW. "Receptor mediated catabolism of plasminogen activators." Awarded By:University of New South Wales. Medical Sciences, 2009. http://handle.unsw.edu.au/1959.4/44489.
Повний текст джерелаRhys-Williams, William. "The microbial catabolism of 4-nitrotoluene." Thesis, Bangor University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359756.
Повний текст джерелаRobertson, Colin Daniel. "Anerobic catabolism of glycerol by Klebsiellae." Thesis, University of Oxford, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.330032.
Повний текст джерелаPlatt, Alison. "A study of 4-hydroxy-2-oxovalerate aldolase from the meta pathway operon of the nah plasmid pWW60-22." Thesis, Bangor University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239887.
Повний текст джерелаWinson, Michael Kenneth. "Molecular biological studies on catabolic plasmids." Thesis, Bangor University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.305927.
Повний текст джерелаBeck, Susan A. "Catabolic factors in tumour-induced cachexia." Thesis, Aston University, 1989. http://publications.aston.ac.uk/12508/.
Повний текст джерелаRafudeen, M. S. "Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052." Doctoral thesis, University of Cape Town, 2001. http://hdl.handle.net/11427/4326.
Повний текст джерелаIncludes bibliographical references.
The substrate basis for the industrial acetone-butanol-ethanol (ABE) fermentations, has been agricultural products rich in starch or sucrose, and employed taxonomically distinct amylolytic and saccharolytic solventogenic clostridial strains respectively. There is evidence to suggest that the utilization of these substrates is subject to carbon catabolite repression. In Gram-positive bacteria, carbon catabolite repression is controlled by a global regulatory mechanism, central to which is an imperfect palindromic sequence, the cre element, which is recognized by a protein of the GalR-LacI family, the CcpA protein. A ccpA homologue, regA, has been previously identified in C. acetobutylicum NCP262 and successfully complemented a B. subtilis ccpA mutant strain. The sucrose operon from C. beijerinckii NCIMB 8052, scrARBK, has been characterised at the physiological and genetic levels with the ScrR repressor found to negatively auto-regulate the operon.
Mayer, Stephen Armond. "Carbon catabolite repression of yeast CBP1 mRNA 3' end formation." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/185336.
Повний текст джерелаFloderus, Eugenie. "Aminopeptidases and arginine catabolism in oral straptococci." Stockholm : Kongl. Carolinska Medico Chirurgiska Institutet, 1990. http://books.google.com/books?id=bMZpAAAAMAAJ.
Повний текст джерелаBroome, Malcolm Charles, and mikewood@deakin edu au. "Aspects of milk protein catabolism by lactobacilli." Deakin University. School of Sciences, 1988. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20050902.120502.
Повний текст джерелаWalters, Nicola Jane. "Arginine and proline catabolism in Schizosaccharomyces pombe." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.257192.
Повний текст джерелаSherburn, Richard. "The microbial catabolism of metalworking fluid additives." Thesis, University of Hull, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301630.
Повний текст джерелаLove, Charmaine. "PrP catabolites as determinants of TSE susceptibility." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5709.
Повний текст джерелаWilkins, M. P. "The role of oxyhaems in haem catabolism." Thesis, Bucks New University, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373588.
Повний текст джерелаSudjadi. "Analysis of cloned genes for GABA catabolism." Thesis, University of Leicester, 1991. http://hdl.handle.net/2381/35163.
Повний текст джерелаLawson, Kathryn René. "Catabolism as a mechanism of polyamine detoxification." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/288919.
Повний текст джерелаKuhn, Hallie. "Regulation of Yolk Catabolism in Early Embryogenesis." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:23845424.
Повний текст джерелаSystems Biology
Hildebrandt, Tatjana [Verfasser]. "Amino acid catabolism in plants / Tatjana Hildebrandt." Hannover : Gottfried Wilhelm Leibniz Universität Hannover, 2019. http://d-nb.info/1204458634/34.
Повний текст джерелаDamaraju, Sridevi. "Analysis of proteins involved in chlorophyll catabolism." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16322.
Повний текст джерелаChlorophyll (Chl) catabolism is characteristically seen during leaf senescence, fruit ripening and seed maturation. Disruption of this coordinated process under frost conditions delays Chl breakdown and is a great concern in rapeseed oil production. The present work addresses this problem by studying the effect of enhanced Chl catabolism in genetically modified tobacco plants. Chl is catabolised to colourless catabolites through a series of enzymatic reactions initiated by Chlorophyllase (Chlase). A water soluble chlorophyll protein (WSCP) has been proposed to transport Chl from thylakoid membranes to the site of action of Chlase. It was assumed that enhancing the gene expression of these early events in Chl catabolism would increase the Chl breakdown process. The present work analysed the overexpression of Chlase from Citrus clementii (CcCHLASE) and WSCP gene from cauliflower (Cau-WSCP) in modified tobacco plants. Initially, the cDNA sequence of CcCHLASE was expressed in E. coli and in vitro tests confirmed the hydrolytic activity of Chlase on Chl. Subsequently, tobacco plants overexpressing CcCHLASE were generated and three T1 lines were analysed at various stress and senescence conditions. The in vivo production of Chlorophyllide (Chlide) indicated the extent of increased Chl breakdown. The Chlase overexpressor lines showed higher Chlide a steady state levels under all tested conditions in comparison to the WT tobacco plants. However, the end catabolites did not show much difference from WT plants. On the other hand, WSCP overexpressor lines did not show any increase in Chlide a levels, but demonstrated an increased protochlorophyllide (Pchlide) levels. This suggested the role of WSCP as a storage molecule of Chl precursors. Additionally, photoprotective function of WSCP was confirmed in WSCP overexpressors, by lower zeaxanthin levels and peroxidase activity even at high light intensities of 700 – 900 µmol photons m-2 s-1 in comparison to the WT tobacco plants.
Durkin, Shannon M. "Complementation of the sor-4 Gene of Neurospora Crassa." Youngstown State University / OhioLINK, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1004562481.
Повний текст джерелаSonoda, Yo. "Structural and functional analysis of a sporulation protein Spo0M from Bacillus subtilis." Kyoto University, 2016. http://hdl.handle.net/2433/215587.
Повний текст джерела0048
新制・課程博士
博士(農学)
甲第19761号
農博第2157号
新制||農||1039(附属図書館)
学位論文||H28||N4977(農学部図書室)
32797
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 三上 文三, 教授 加納 健司, 教授 喜多 恵子
学位規則第4条第1項該当
La, Cecilia Daniele. "Comprehensive modeling of agrochemicals biodegradation in soil: A multidisciplinary approach to make informed choices to protect human health and the environment." Thesis, The University of Sydney, 2019. http://hdl.handle.net/2123/20691.
Повний текст джерелаHorton, William Henry Clay. "Characterization of the Components of Carbon Catabolite Repression in Clostridium perfringens." Thesis, Virginia Tech, 2004. http://hdl.handle.net/10919/36119.
Повний текст джерелаMaster of Science
Sabiston, C. Paul. "The role of catabolin in experimental osteoarthritis." Thesis, University of British Columbia, 1985. http://hdl.handle.net/2429/24909.
Повний текст джерелаMedicine, Faculty of
Graduate
Lemley, Caleb Owens. "Alterations in progesterone catabolic enzymes by insulin." Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5286.
Повний текст джерелаYang, Yifan. "Catabolic responses to resistance exercise in humans." Virtual Press, 2005. http://liblink.bsu.edu/uhtbin/catkey/1317922.
Повний текст джерелаPeel, Michelle C. (Michelle Carolyn) Carleton University Dissertation Biology. "Catabolic genotype distributions in the Niagara watershed." Ottawa, 1996.
Знайти повний текст джерелаSteward, W. P. "The structure of proteoglycans associated with normal and malignant cells." Thesis, University of Manchester, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234215.
Повний текст джерелаHou, Chunsheng 1968. "Sulfur amino acid catabolism in a piglet model." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=78381.
Повний текст джерелаCarvallo, Sergio Luis Fuenmayor. "Catabolism of naphthalene by Pseudomonas sp. strain U2." Thesis, Bangor University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263318.
Повний текст джерелаYang, M. "Catabolism and bioactivity of bradykinin and related peptides." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.557857.
Повний текст джерелаJames, V. J. "Regulation of xenobiotic catabolism in plant tissue culture." Thesis, Cardiff University, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380205.
Повний текст джерелаLe, Tissier Paul Roussel. "The biochemical genetics of purine catabolism in mice." Thesis, University of Reading, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236393.
Повний текст джерелаWade, David Patrick. "Receptor-mediated catabolism of lipoproteins by the liver." Thesis, Imperial College London, 1989. http://hdl.handle.net/10044/1/47700.
Повний текст джерелаRaucoules, Daniel. "Catabolites plasmatiques des monoamines et depressions : etude preliminaire." Aix-Marseille 2, 1989. http://www.theses.fr/1989AIX20954.
Повний текст джерелаUmmadi, Madhavi. "Tryptophan Catabolism in Brevibacterium linens BL2." DigitalCommons@USU, 2002. https://digitalcommons.usu.edu/etd/5501.
Повний текст джерелаDebailleul, Fabien. "A new expression system in Saccharomyces cerevisiae based on nitrogen catabolite regulation." Doctoral thesis, Universite Libre de Bruxelles, 2013. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209428.
Повний текст джерелаGap1, the general amino acid permease of Saccharomyces cerevisiae, is a plasma membrane protein which is synthesized and most active under conditions of poor nitrogen supply. Under these conditions, the role of Gap1 is to scavenge external amino acids to be used as nitrogen sources or directly as building blocks for protein synthesis. Gap1 is a member of the Yeast Amino Acid Transporter (YAT) family, a family of amino acid transporters highly conserved in bacteria and fungi.
The intracellular trafficking of Gap1 has been the subject of intense investigation as well as the role of lipids (in particular sphingolipids) in its activity and folding. These studies have all been carried out in the cellular context using versatile yeast genetics as exploratory tools. While such in vivo investigations allow to identify physiologically relevant features, they do not provide the details to understand the molecular basis of these phenomena. In order to decipher the molecular features responsible for biological functions, physiological analysis must be combined with biochemical, biophysical and structural studies which typically will be performed on isolated and purified proteins.
Our work during this study fits in this trans-disciplinary approach that aims at understanding different properties of the protein: (I) how sphingolipids modulate the activity of Gap1, (II) what part of the protein, and more specifically, what residues, are implicated in its regulation and (III) what are the molecular determinants of the multi-specificity of Gap1.
At the beginning of this work, Gap1 had not been previously produced and purified. During this thesis we have identified suitable expression and purification strategies for Gap1 and initiated first characterization studies. In the process, we have developed a new expression system in S. cerevisiae based on the nitrogen catabolite repression.
The capacity of this system to express other proteins was successfully tested for two other yeast transporters (Mep2 and Uga4) and for two human proteins: MD-2, a soluble protein and Vglut1, a vesicular transporter.
Therefore, we propose our expression design as a viable alternative to existing systems of production in yeast and as a valuable tool to be tested when starting the expression of a new target.
Doctorat en Sciences
info:eu-repo/semantics/nonPublished
DeGrazia, Henry. "A biophysical investigation of the mechanisms of the catabolite gene activator protein." Diss., Georgia Institute of Technology, 1988. http://hdl.handle.net/1853/25641.
Повний текст джерелаO'Donnell, Kevin John. "Studies on the behaviour of catabolic plasmid pWW15." Thesis, Bangor University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280644.
Повний текст джерелаWhite, Tommi Anna. "Structural and functional studies of proline catabolic enzymes." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4760.
Повний текст джерелаThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on March 24, 2009) Vita. Includes bibliographical references.