Добірка наукової літератури з теми "Capteur d'affinité"
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Дисертації з теми "Capteur d'affinité"
Gandar, Aude. "Échantillonnage et détection des filtres solaires, nouveaux polluants des eaux du littoral méditerranéen." Electronic Thesis or Diss., Perpignan, 2023. http://www.theses.fr/2023PERP0008.
Повний текст джерелаUV filters are part of the emerging contaminants causing a risk to aquatic environments. Quantification of those molecules usually uses chromatographic technics. A method based on spectroelectrochemistry was developed, it is based on the combined use of an electrochemical experiment, chronoamperometry, and UV spectrophotometry. Some UV filters’ spectrum are modified following oxidation. The developed method enable the recording of UV spectra before and after potential application, set at +1,8 V vs Ag during 30 min. Deconvolution using both spectra is then performed to simultaneously identify and quantify four UV filters. This method was developed for the analysis of avobenzone, octinoxate, octocrylene and oxybenzone. In addition to the analytical method, a passive sampling experiment was performed in Mediterranean waters. Among the studied UV filters, bis-ethylhexyloxyphenol methoxyphenyl triazine, ethylhexyl triazone, octocrylene and diethylamino hydroxybenzoyl hexyl benzoate were measured at concentration in the µg/L range. A risk assessment on Mediterranean and tropical species showed a medium to high risk for many species
Ben, Jrad Amani. "Nouvelles sondes électrochimiques pour le marquage de biocapteurs d'affinité." Thesis, Perpignan, 2019. http://www.theses.fr/2019PERP0038.
Повний текст джерелаNowadays, drug residues present a new threat as pllutant of water resources. In 2015, two estrogenic hormones: estradiol and ethinyl estradiol, were mentioned on the watch list established in support of European environmental legislation (Water Framework Directive), which aims to protect the water quality. In this context, we have developed a redox-labeled electrochemical aptasensor for the detection of these pharmaceutical substances in water. These sensors are based on the use of an estradiol-specific aptamer as a recognition element and electroactive metal complexes as redox probes. These complexes are formed by a tetradentate ligand (N2O2) of Salen / Salan type linked to a maleimide functional group. This maleimide moiety was essential for the attachment of redox-complex to the aptamer previously modified with a thiol group. Copper and oxovanadium complexes have been synthesized and characterized showing interesting electrochemical properties. The labeling of the aptamer by these complexes highlighted the instability of the salen ligand compared to its reduced derivative, the salan ligand. The design of the aptasensor was achieved by immobilizing the oxavanadium salan-labeled aptamer on a screen-printed carbon electrode. The results of estradiol detection showed that the sensor behaves as an ON / OFF system and that the output signal is corrolated to the concentration of the target in the sample. This new type of aptamer labeling, developed in this thesis, offers an alternative to con. The labelling method developed in this thesis offers an alternative to classical techniques and opens new perspectives for the development of new affinity sensors
Brouchon, Julie. "Chromatographie cellulaire d'affinité : étude expérimentale des mécanismes de capture spécifique et implications pour un développement industriel." Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066335/document.
Повний текст джерелаDevelopment of cell sorting system based on affinity chromatography for medical application is the goal of this thesis. We study mechanisms responsible for specific cell capture in chromatographic column. First an experimental study on system model emphasize the importance of the transport step, which allows the encounter between cells and surface of capture. Cell sedimentation in the main way of transport. That means flow velocity is a key parameter to optimize this transport step. Then the whole cell capture is studied : encounter and specific link formation. Depending on the flow velocity, there are two regimens. Until a velocity of 10-3 m.s-1, kinetics of capture is governed by encounter kinetics. For higher velocity, only fraction of encounters leads to cell capture. The capture kinetics depends not only on transport, but also on kinetics of formation of specific link. This experimental study allows us to design cell affinity chromatography depending on the need of each application. Especially, cell sorting at industrial scale is conceivable concerning the separation duration and column dimensions
Robin, Maëlenn. "Capture biomoléculaire impliquée dans la reconnaissance moléculaire supportée : modélisation et caractérisation expérimentale." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSE1066.
Повний текст джерелаSolid-phase immunoassays are used for in vitro diagnostic to detect the presence or measure the concentration of a molecule of interest in a biological sample. They rely on the specific interaction between an antigen and an antibody. Usually, antibodies specific to the antigens to be detected are immobilized on a solid surface to capture the antigens of interest and separate them from the rest of the sample components. During solid-phase immunoassay development, sensitivity, specificity and time-to-result need to be optimized through the choice of dedicated ligands, solid supports, flow rates,… Classically, these choices are made empirically. A better understanding and prediction of the complex molecular interactions that occur in the different steps of a diagnostic immunoassay is likely to be useful to: identify the critical parameters of immunoassays, simplify and speed-up the process of identification of the best immunoassay conditions and improve the immunoassays currently available. The VIDAS® instrument, commercialized by bioMérieux is one of the most widely used immunoassay system in clinical laboratories worldwide. In this PhD work, two experimental tools based on inverse chromatography are built and tested. A predictive model of antibody/antigen interaction kinetics in immunoassays is developed. The experimental tools which mimic VIDAS® process conditions are used to validate the predictive model and to estimate model parameters characterizing antibody/antigen interaction kinetics from experimental curves. In the future, based on the results, one of the experimental tools associated with the model could be used by bioMérieux to design immunoassay systems
Li, Bin. "Molecularly imprinted polymers for applications in cosmetology." Thesis, Compiègne, 2013. http://www.theses.fr/2013COMP2083.
Повний текст джерелаMolecularly imprinted polymers (MIPs) are tailor-made synthetic receptors possessing specific cavities for a given target molecule. They are produced by introducing, into the polymer precursors, guest molecules that act as templates at the molecular level. Interacting and cross-linking monomers are then copolymerized to form a cast-like shell. After removal of the template, cavities complementary to the template in size, shape and position of functional groups are revealed in the polymer, which can now specifically bind the template. Thanks to these specific molecular recognition properties, MIPs have found applications in areas like bio sensors, solid phase extraction, affinity chromatography, catalysis, and drug delivery. Although the MIP concept originated from imprinted silica in the 1930s, imprinted sol-gel materials received little attention afterwards due to the introduction of the more versatile organic polymers as imprinting matrix. However, compared to organic polymers, sol-gels possess higher thermal stability, better water compatibility and larger inner surface area. There have been many applications to biomolecules in aqueous conditions with sol-gel imprinting materials. In this thesis, we have developed organic and silica sol-gel MIPs for applications in cosmetics and drug delivery. MIPs able to adsorb the dandruff-inducing molecule oleic acid (OA) were produced via both the organic and inorganic routes. In the organic MIPs synthesis, different positively charged monomers were used, one of which, acryloyl aminobenzamidine, was specifically synthesized. Although some binding of oleic acid was obtained, specificity and capacity of these polymers were not satisfying. Sol-gel MIPs, on the other hand, exhibited good specific recognition and high binding capacity for OA. A MIP of the composition OA:APTES:TEOS= 1:1.6:1.7 yielded a capacity of 625 μmol.g-1 in artificial sebum. Furthermore, tests were carried out to capture OA on stratum corneum and reconstructed skin (Episkin). Less penetration of OA was observed in the presence of a MIP than with a non-imprinted control polymer. Deodorant materials are another topic of this thesis. MIPs that are able to adsorb certain precursors of odorant molecules, the glutamine conjugates of (E)-3-methyl-2-hexenoic acid (3M2H) and 3-hydroxy-3-methyl-hexanoic acid (3H3MH) were prepared. N-hexanoyl glutamine and N-hexanoyl glutamate were used as templates. After optimization of the MIP composition, we found that MIPs synthesized with acryloyl aminobenzamidine as functional monomer had the highest adsorption capacity for N-hexanoyl glutamine, and also recognised the glutamine targets of 3M2H and 3H3MH. Some preliminary promising binding results were obtained in artificial sweat. The third part of this work concerns a drug delivery MIP. Salicylic acid (SA) is a drug used to treat acne. SA-imprinted polymers were prepared via both organic imprinting and the sol-gel process.Compared to organic MIPs, sol-gel MIPs have a higher capacity, 180 μmol.g-1, and 7 times higher binding than to a non-imprinted control polymer was observed. Release tests were carried out in different aqueous media, the most efficient drug release was observed in pure water. In conclusion, applications of molecularly imprinted polymers for cosmetics and drug delivery have been investigated. Our results demonstrate the great potential of in particular sol-gel MIPs for these purposes