Дисертації з теми "CAMP assay"
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Lupica, Samuel J. "Nitrate Toxicity to Common Carp Measured Noninvasively by Novel Enzyme-linked Immunosorbent Assay for Cortisol." University of Toledo Health Science Campus / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=mco1226956326.
Повний текст джерелаPandrangi, Raj Gopal. "Determination of genotoxic stress in feral populations of bullheads and carp using the alkaline single cell gel assay." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape16/PQDD_0022/NQ30295.pdf.
Повний текст джерелаCamara, Ramatoulie. "Design, synthesis and biological evaluation of potential inhibitors of S100P, a protein implicated in pancreatic cancer." Thesis, University of Hertfordshire, 2015. http://hdl.handle.net/2299/17117.
Повний текст джерелаTeixeira, Mirian Vieira. "Análise da expressão e das interações da subunidade catalítica da PKA do fungo patogênico Paracoccidioides ssp." Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/5811.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
The Paracoccidioides genus comprises a complex of pathogenic fungi that are the etiologic agents of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in Latin America. The infection begins after inhalation of fungal propagules, which reach the epithelium of the alveoli where the transition from the mycelial to the pathogenic yeast form. Host elevated temperature triggers the morphological switch, which is necessary for fungal pathogenicity. The cAMP/protein kinase A (PKA) signaling pathway has been shown to be important in controlling morphological changes and the pathogenicity of several pathogenic fungi. Evidence also highlights the importance of the cAMP/PKA pathway in the morphological transition of Paracoccidioides. PKA is the major effector of this signaling pathway. The protein is an inactive tetramer composed of regulatory subunit, encoded by the BCY1 gene; and catalytic subunit, encoded by the TPK2 gene. Upon binding of cAMP to the regulatory subunits, the catalytic subunits dissociate and become active. Activated PKA subsequently phosphorylates protein kinases, transcription factors, and other substrates to control several biological processes. In this study, we evaluated the expression and interactions of Tpk2 protein Paracoccididioides spp. The Tpk2 is present in mycelium decreased during the initial stages of transition phases, and increases again at the end of differentiation, with maximal levels in yeast. We analyzed the interactions of recombinant Tpk2p with Paracoccidioides proteins using pull-down assays followed by MS analysis. Two interacting proteins were identified: the heat shock protein 90 (Hsp90) and a conserved hypothetical protein with a MFS domain. Hsp90 is involved in the regulation of morphogenesis, development and virulence in several thermal dimorphic fungi. These data are important for understanding the mechanisms that trigger the transition phases in Paracoccidioides.
O gênero Paracoccidioides compreende um complexo de fungos patogênicos, que são os agentes etiológicos da paracoccidioidomicose (PCM), a micose sistêmica mais prevalente na América Latina. A infecção inicia-se com a inalação de propágulos do fungo, que atingem o epitélio dos alvéolos pulmonares, onde ocorre à transição da forma de micélio para a forma patogênica, de levedura. Há evidências de que a temperatura seja o principal fator responsável pela diferenciação celular desses fungos, e sua patogenicidade é frequentemente associada com a transição dimórfica. A via de sinalização cAMP/ proteína quinase A (PKA) controla alterações morfológicas e de virulência/patogenicidade em várias espécies de fungos patogênicos humanos. Evidências apontam também para a importância da via cAMP/PKA em Paracoccidioides spp. A PKA é o principal efetor desta via de sinalização. A proteína na forma inativa é um tetrâmero composto de subunidade regulatória, codificada pelo gene BCY1; e subunidade catalítica, codificada pelo gene TPK2. Após a ligação de cAMP às subunidades regulatórias, as subunidades catalíticas dissociam-se e tornam-se ativas. Ativada a PKA fosforila proteína-quinases, fatores de transcrição, e outros substratos para controlar diversos processos biológicos. Neste estudo, avaliamos a expressão e as interações da proteína Tpk2 de Paracoccididioides spp. A Tpk2 está presente em micélio, diminui nos estágios iniciais da transição de fases e volta a aumentar no final da diferenciação, apresentando níveis máximos na levedura. Foram analisadas as interações de Tpk2p recombinante com proteínas de Paracoccidioides utilizando ensaios de pull-down, seguido por análise de MS. Foram identificadas duas proteínas que interagem: a proteína de choque térmico 90 (Hsp90) e uma proteína hipotética conservada com um domínio MFS. Hsp90 está envolvido na regulação da morfogênese, desenvolvimento e virulência em vários fungos dimórficos térmicos. Estes dados são importantes para entendimento dos mecanismos que disparam a transição de fases em Paracoccidioides spp.
Suntharalingam, Gaayathiri [Verfasser]. "Der Einfluss der Induktion von Tumornekrosefaktor α und Transforming-Growth-Factor β auf die epithelial-mesenchymale Transition oraler Plattenepithelkarzinome im CAM-Assay / Gaayathiri Suntharalingam". Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://d-nb.info/1228364583/34.
Повний текст джерелаLeupold, Jörg. "Untersuchungen zur Regulation des Invasionsgens Urokinase-Rezeptor (CD87) durch den Tumorsuppressor Pdcd4 unter gleichzeitiger methodischer Neuetablierung eines quantitativen CAM-Assays." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-36138.
Повний текст джерелаRuhe, Larissa. "Investigation of cap-independent translation initiation in neuronal differentiation." Doctoral thesis, Humboldt-Universität zu Berlin, 2020. http://dx.doi.org/10.18452/21184.
Повний текст джерелаTranslation initiation is a complex and highly regulated process which involves the assembly of an elongation competent ribosome on the mRNA. The vast majority of eukaryotic mRNAs is translated by a canonical cap-dependent mechanism. This requires the eIF4F protein complex to bind the mRNA at the 5’-cap to recruit further eIFs and the small ribosomal subunit which then scans the 5’UTR in 5’ to 3’ direction until a start codon is encountered. Afterwards the large ribosomal subunit joins and protein synthesis begins. Besides that, translation of mRNAs can be mediated by IRESs, internal ribosome entry sites, which recruit the ribosome in a cap and 5’-end-independent manner to the start codon. Such cellular IRES-mediated translation is thought to be inefficient under physiological conditions but activated during stress. As the regulation of this mechanism is not well understood, we aimed to elucidate cellular cap-independent translation events. Therefore, we generated a mouse embryonic stem cell line with inducible overexpression of a dominant negative mutant of 4E-BP1. 4E-BP1 sequesters the cap-binding protein eIF4E so that the eIF4F protein complex fails to assemble at the 5’-cap. We performed shotgun proteomics during 4E‑BP1 overexpression and neuronal differentiation to globally monitor translation dynamics. Genes with reduced sensitivity for cap-dependent translation were identified and tested for internal translation initiation in bicistronic reporter assays. After stringent validation one cap-independently translated mRNA, Pqbp1, was discovered. The second part of this study investigated cap-independent translation initiation on a circRNA, which by nature lacks free ends and thus requires IRES-mediated translation. We could show that circMbl is translated in vitro and thus contributed to the scientific evidence for the translation of circRNAs in fly brain, which was studied in a collaboration project.
Amui, Saulo França. "Do laboratório ao campo virtual: desenvolvimento de um banco de dados de venenos de serpentes brasileiras e análise computacional de estruturas primárias de fosfolipases A2." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/60/60134/tde-21052007-094343/.
Повний текст джерелаTechnological advances have been contributing, more and more, with biological and scientific areas, offering computational tools and specific systems which in silico data analysis supply reliable and fast results. The present project considers the development of an Internet portal for installation and use of laboratory data base for the main Brazilian serpents, with its respective venom and natural anti-venom, and the analysis of obtained data in pharmacological assays and biochemists. Using the easiest way of communication and interaction, the Internet allows sharing of data and information between communities of researchers around the world, especially for Brazilian researchers, making resources and time possible. Elementary data about serpents have been stored in the data base, as well as toxic, pharmacological and enzymatic activities of venom components, besides biotechnological applications of the products that can be obtained from these venom, enclosing clinical data and statistical values of ophidian accidents. Biochemists aspects of the assays carried through in laboratory allowed the construction of a comparative analysis tool for primary structures of PLA2s, deposited in international data bases. Beyond interactivity between researchers, in discussion forums, the system counts with lists of main articles published in indexed periodic, duly and constantly updated with bibliographical revisions.
Hirtzlin-Pinçon, Olivier. "L'influence de la situation géopolitique au Moyen-Orient sur la génération des accords israélo-arabes depuis "Camp David I" : la frontière d'Israël." Phd thesis, Université des Sciences Sociales - Toulouse I, 2008. http://tel.archives-ouvertes.fr/tel-00300769.
Повний текст джерелаTrotman, Jackson B. "New Insights into the Biochemistry and Cell Biology of RNA Recapping." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523896565730483.
Повний текст джерелаHsu, Jou-Ping, and 許柔平. "The immunochemical assay of the carp zinc-binding protein." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/72631260846762115436.
Повний текст джерела國立臺灣海洋大學
食品科學系
97
Abstract High concentration of zinc was found in the digestive tract tissue of common carp which was not seen in other fish species. A zinc-binding protein with a molecular weight of 43kDa located on the plasma membrane of the connective tissue cells was responsible for the high zinc in common carp. Using the “carp zinc-binding protein” as antigen, polyclonal antibody of the protein was prepared. An immunochemical assay method using the antibody against the “carp zinc-binding protein” to quantitatively determine the amount of the protein in fish tissue was developed to study the existence of the protein in fish tissue. The immunochemical assay method developed could specifically detect a amount of “carp zinc-binding protein” between 2-40 ng. Based on this immunochemical assay, it was found that common carp had high amount of “carp zinc-binding protein” in its digestive tract tissue, kidney, head kidney, and blood with values of about 2, 1, 12, 7, and 1 mg/(g fresh tissue), respectively; but lower amount in its heaptopancreas and muscles (<0.20mg/ �� g fresh tissue ��). In the other fishes, crucian carp had high amount of “carp zinc-binding protein” in its kidney, head kidney and spleen, but grass carp, silver carp and tilapia have little or no “carp zinc-binding protein” (<0.04 mg/ �� g fresh tissue ��) in their tissues. The zinc and “carp zinc-binding protein” in the tissues of common carp fed basal diet was compared with those fed high zinc diet. It was found that the amount of zinc and “carp zinc-binding protein” in the digestive tract tissue and head kidney increased in the common carp fed high zinc diet. The zinc concentration in the blood of the common carp didn’t increase after feeding high zinc diet, but the amount of “carp zinc-binding protein” increased. This immunochemical assay method might be used to study the existence and distribution of “carp zinc-binding protein” in other organism. Besides, using immunochemical assay to determine the change of the “carp zinc-binding protein” in common carp under different circumstances or conditions might be very useful in understanding the function of “carp zinc-binding protein” and its physiological meaning.
"The development of a sandwich ELISA for grass carp growth hormone and generation of 4 cysteine recombinant grass carp growth hormone." 1998. http://library.cuhk.edu.hk/record=b6073072.
Повний текст джерелаThesis (Ph.D.)--Chinese University of Hong Kong, 1998.
Includes bibliographical references (p. 158-168).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Mode of access: World Wide Web.
Abstracts in English and Chinese.
Ueberdiek, Stefan. "Einfluss von LEF1 auf das Tumorwachstum im Burkitt-Lymphom-Xenograft-Modell." Doctoral thesis, 2016. http://hdl.handle.net/11858/00-1735-0000-002B-7CB1-4.
Повний текст джерелаVágnerová, Lenka. "Využití testu CAM pro charakterizaci a studium invazivních vlastností rakovinných buněk." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-312678.
Повний текст джерелаSuntharalingam, Gaayathiri. "Der Einfluss der Induktion von Tumornekrosefaktor α und Transforming-Growth-Factor β auf die epithelial-mesenchymale Transition oraler Plattenepithelkarzinome im CAM-Assay". Doctoral thesis, 2021. http://hdl.handle.net/21.11130/00-1735-0000-0005-1567-0.
Повний текст джерелаBlumberg, Alina Friederike. "Vergleichende Analysen von drei verschiedenen Burkitt-Lymphom-Zelllinien im CAM-Xenograft-Modell unter besonderer Berücksichtigung des Transkriptionsfaktors LEF1." Doctoral thesis, 2018. http://hdl.handle.net/11858/00-1735-0000-002E-E4CD-8.
Повний текст джерелаHillertz, Per [Verfasser]. "Advances in fragment-based drug discovery : studies of cAMP-dependent protein-kinase A using X-ray crystallography, surface plasmon resonance and high compound concentration assays / presented by Per Hillertz." 2010. http://d-nb.info/1003221165/34.
Повний текст джерелаWilming, Pia Josefa. "Untersuchungen zur Angiogenese des Burkitt-Lymphoms unter besonderer Berücksichtigung des Lymphocyte enhancer-binding factor-1." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F55-9.
Повний текст джерелаLeupold, Jörg [Verfasser]. "Untersuchungen zur Regulation des Invasionsgens Urokinase-Rezeptor (CD87) durch den Tumorsuppressor Pdcd4 unter gleichzeitiger methodischer Neuetablierung eines quantitativen CAM-Assays / vorgelegt von Jörg Leupold." 2004. http://d-nb.info/975149989/34.
Повний текст джерелаWaszczuk, Małgorzata. "Syntetyczne analogi trimetyloguanozyno kapu modyfikowane w mostku trifosforanowym jako narzędzia do badania snurportyny i optymalizacji sygnału transportu dojądrowego dla czynników terapeutycznych." Doctoral thesis, 2017. https://depotuw.ceon.pl/handle/item/2426.
Повний текст джерела