Дисертації з теми "BSOA"
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Hung, Jacky. "The Cystine Binding Protein (BspA) of Lactobacillus fermentum BR11." Thesis, Queensland University of Technology, 2005. https://eprints.qut.edu.au/16146/1/Jacky_Hung_Thesis.pdf.
Повний текст джерелаHung, Jacky. "The Cystine Binding Protein (BspA) of Lactobacillus fermentum BR11." Queensland University of Technology, 2005. http://eprints.qut.edu.au/16146/.
Повний текст джерелаSoutar, Colin. "Optical information processing using photorefractive BSO." Thesis, Abertay University, 1991. https://rke.abertay.ac.uk/en/studentTheses/a757b4d3-6c1e-4600-aed8-430e7078c6c5.
Повний текст джерелаCook, Nicholas James. "Properties and processing applications of photoreactive BSO." Thesis, University of Abertay Dundee, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242115.
Повний текст джерелаForsyth, Robert Bruce. "A study of auto-anti-idiotypes to BSA." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27425.
Повний текст джерелаScience, Faculty of
Microbiology and Immunology, Department of
Graduate
Wang, Zhao Qi. "Real-time optical intensity correlation using photorefractive BSO." Thesis, Abertay University, 1995. https://rke.abertay.ac.uk/en/studentTheses/f1330975-bc23-4532-ac7b-8aeb9cad8c81.
Повний текст джерелаLin, Chenting. "Single crystal growth and characterization of BSO (Bi12SiO20)." Thesis, Massachusetts Institute of Technology, 1994. http://hdl.handle.net/1721.1/11647.
Повний текст джерелаHecker, Dominic, Daniel Gloess, Peter Frach, and Gerald Gerlach. "Electrospray ionization deposition of BSA under vacuum conditions." SPIE, 2015. https://tud.qucosa.de/id/qucosa%3A35187.
Повний текст джерелаKawamura, Keiichi. "BSO - Broad System of Ordering: an international bibliography." K. Kawamura, 2011. http://hdl.handle.net/10150/129413.
Повний текст джерелаThis bibliography,compiled by K. Kawamura, lists about 270 references to BSO ranging from 1973 to 2010. The number of languages covered in the bibliography is 19 in all: Arabic, Chinese, Croatian, Czech, Dutch, English, French, German, Hungarian, Italian, Japanese, Korean, Lithuanian, Polish, Portuguese, Russian, Slovak, Slovenian and Spanish. Every item has English abstract and/or annotation. Items are arranged in systematic order, and cross-references among related items as well as author and language indexes complement the systematic arrangement.
Carvalho, Jesiel Freitas. "Crescimento e caracterização de monocristais fotorreativos: BSO e BTO." Universidade de São Paulo, 1994. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-14012009-093345/.
Повний текст джерелаIn this work we grew Bi12SiO20 (BSO) and Bi12TiO20 single crystals. The BSO crystals have been grown from the melt composition of 6Bi2O3:1SiO2 by the Czochralski method. The best results were obtained at pulling rates from 1 to 2.5m/h, the rotation rate of 20rpm was constant. The BTO crystals have been grown by the top-seeded solution growth technique from a 10Bi2O3:1SiO2 solution with pulling rates less than 0.3mm/h and rotation rates from 16 to 30rpm. The crystal quality was examined by optical and scanning electron microscopy, selective etching, and x-ray diffraction. We identified the macroscopic defects by optical microscopy and discussed their nature and probable origin. Using selective etching, we analyzed the etching pits morphology and evaluated the dislocation density. To identify stoichiometric defects, composition measurements by electron probe microanalysis were made. To obtain crystallographic characterization, we calculated the cell parameter by powder method x-ray diffraction and used the Rietveld method to verify the crystallographic structure. And, also, we measured the optical activity, a constant of the crystals.
Jeffrey, Philip Michael. "Enhancement of the photorefractive properties of BSO and BaTiO3." Thesis, University of Southampton, 1994. https://eprints.soton.ac.uk/399145/.
Повний текст джерелаGouveia, Duarte Domingos. "Influência da oxidação da BSA na sua digestão proteolítica." Master's thesis, Universidade de Aveiro, 2012. http://hdl.handle.net/10773/11360.
Повний текст джерелаAs modificações oxidativas em proteínas são processos comuns em organismos aeróbios. A compreensão dos processos envolvidos na iniciação e desenvolvimento destas modificações, assim como da sua natureza, poderá contribuir para a elucidação do processo de envelhecimento, assim como de algumas patologias a ele associadas. A análise de proteínas oxidadas é comummente feita com base em técnicas proteómicas de identificação e quantificação utilizando a espectrometria de massa. A identificação é feita normalmente utilizando a análise dos péptidos resultantes de digestões proteolíticas das proteínas. No entanto, existem muitas limitações impostas pelas modificações oxidativas na utilização destas técnicas. De entre elas, a digestão enzimática a que as proteínas são sujeitas antes da análise por MS, representa talvez a que necessita de mais atenção. A ocorrência de clivagens falhadas poderá levar à impossibilidade de efetuar identificações e quantificações eficazes. Neste trabalho pretendeu-se avaliar a influência que a oxidação da albumina do soro bovina (BSA) teria na sua digestão proteolítica. Para tal procedeu-se à oxidação da BSA recorrendo-se à reação de Fenton durante vários tempos de incubação. Analisou-se posteriormente o grau de oxidação avaliando as concentrações de proteína por SDS-PAGE, os níveis de carbonilos em solução por espectrofotometria e na banda da BSA por Western Blot. A eficiência da digestão proteolítica foi avaliada por espectrometria de massa com base nas taxas de cobertura obtidas em pesquisas dos espectros em bases de dados. No final, concluiu-se que a proteína sofre uma fragmentação crescente ao longo do processo de oxidação, em consonância com um aumento crescente dos níveis de carbonilos. Pela análise da eficiência da digestão, não se verificaram diferenças entre as digestões de amostras não oxidadas e oxidadas, levando-nos a concluir que a oxidação da proteína não influencia a sua digestão com tripsina ou proteinase K.
Protein oxidative modifications are a common process in aerobic organisms. The comprehension of the processes involved in the initiation and development of these modifications can give us better insights of some common pathologies associated with the aging process. The analysis of oxidized proteins is normally based on mass spectrometric proteomic methodologies. The identification is performed with a shotgun strategy, where the protein mixture is submitted to proteolysis and the resulting peptides are analysed on a mass spectrometer. However, caution should be taken in applying these techniques in the field of protein oxidation. Oxidative modifications impose several limitations to protein identification and quantification due to the several structural and chemical modifications that occur. The enzymatic proteolysis applied before MS analysis is probably the step where one has to be especially careful, due to the occurrence of missed cleavages that difficult an efficient quantification. In this work, it was evaluated the efficiency of the proteolytic digestion with trypsin and proteinase K on oxidized bovine serum albumin (BSA). The protein was submitted to a metal-catalysed oxidation system for several incubation periods and the extent of oxidation was analysed by determining its concentration on SDS-PAGE, and the carbonyl levels by spectrophotometry and Western blot. In order to evaluate the digestion efficiency a mass spectrometry analysis based on sequence coverage of the protein after database search was carried out. We concluded that the protein suffers fragmentation during the oxidative reaction along with a substantial raise of amino acid residues side chain carbonyl levels. The digestion efficiency of the control and oxidized sample was similar, leading us to conclude that the oxidative state of the protein wasn’t an obstacle to the proteases.
Sá, Antonio Wellington Lima de. "Fabricação e caracterização de filmes automontados de PAH/BSA." reponame:Repositório Institucional da UFABC, 2010.
Знайти повний текст джерелаFerreira, Ernando Silva. "Interação da proteína albumina do soro bovino (BSA) com substratos sintéticos." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/59/59135/tde-22072010-102300/.
Повний текст джерелаThe interface formed by biological materials and synthetic materials has great importance in biomedical applications such as the development of biomaterials for medical implants, which has as an essential process of protein adsorption on the surface of biomaterials, and is not yet well understood in the molecular level. Some proteins undergo conformational changes after adsorption at solid-liquid interfaces, affecting their functions or properties, and few techniques can measure conformational changes in solid interfaces. It is possible to study the intrinsic fluorescence of proteins: the position of the maximum in the spectral range of fluorescence, the quantum efficiency and lifetime of fluorescence are indicators of change in the local environment of fluorescent groups of protein molecules. On the other hand, gold nanopartículas have attracted much attention for its affinity with biological materials and their optical properties. In this thesis we study the feasibility of glass substrates, quartz, mica and ITO (Indium tin oxide) modified with chitosan, phtalocyanines (Ni, Fe and Ni) and poly (allylamine hydrochloride) (PAH) on the adsorption of BSA in the form of films produced by the layer by layer technique. The system was studied by UV-Vis and static and time-resolved fluorescence spectroscopy. Morphological characterization of the films was performed by atomic force microscopy and optical microscopy. The results indicate that the films of BSA/PAH grew with efficiency four times greater than the films made of chitosan, that the quartz has the best working window for UV-vis and there is a relationship between the pH of the BSA and lifetime of fluorescence of the resulting film. Gold nanoparticles were produced by chemical reduction and stabilized by four different methods. The growth of nanoparticles was monitored by UV-vis spectroscopy. The surface charge of nanoparticles and the BSA was estimated at various pH values by zeta potential measurements. The results indicated that the nanoparticles have negative charges in the pH range studied. BSA solutions were prepared at various pH values, were taken to interact with gold nanoparticles. Fluorescence quenching data of BSA showed a greater affinity of the BSA with nanoparticles stabilized with sucrose, at pH near the isoelectric point (IEP) estimated for BSA.
Istanbullu, Alexander. "Prestandajämförelse av textbaserade och binära dataformat för AJAX." Thesis, Högskolan i Skövde, Institutionen för kommunikation och information, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-8509.
Повний текст джерелаOstrowski, Adam. "Characterisation of the localisation and function of the Bacillus subtilis YuaB protein during biofilm formation." Thesis, University of Dundee, 2012. https://discovery.dundee.ac.uk/en/studentTheses/29fe0d1b-a010-4cb9-8698-7f393b6e08ee.
Повний текст джерелаGlascock, Abigail L. "Evolution and Niche Specialization of Microbial Taxa in Vaginal Infection and Pregnancy." VCU Scholars Compass, 2018. https://scholarscompass.vcu.edu/etd/5385.
Повний текст джерелаSales, Elisa Morandé. "Estudo das interações proteína-proteína, proteína-membranas e proteína-agentes desnaturantes por espalhamento de raios-X a baixos ângulos." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-22052018-152931/.
Повний текст джерелаIn this work we have used small-angle x-ray scattering (SAXS) to study four systems of biological interest. We aim to investigate the self aggregation of proteins and protein complexes that would form amyloid fibers; protein/protein interaction, simulating high concentrations; protein/cell-membrane interaction, simulating extracellular matrix vesicles (MVs) from biomineralizing systems; and protein/denaturating-agents interactions. On the case of amyloid formation, we have investigated the aggregation of G-domain of septin-6 (SEPT6G) and the protein complex formed with G-domain of septin-2 (SEPT2G-SEPT6G). At temperatures lower than 15°C, both SEPT6G and SEPT2G-SEPT6G were found predominantly as dimers. At 25°C, SEPT2G-SEPT6G heterodimer is still stable while aggregates of SEPT6G grow. Both coexist in solutions of SEPT2G-SEPT6G dimers, with the percentage of dimers decreasing the higher the temperature. As for the study of MVs, we have shown that DPPC and DPPC:DPPS (9:1) liposomal mimetics have the same structural characteristics at the absence or presence of Calcium. The interaction with human annexin V protein (A5), related to biomineralization processes, affects the model membrane by the creation of nanopores. The addition of tissue-nonspecific alkaline phosphatase (TNAP) does not change the structural properties of the proteoliposome when A5 is present. The addition of SDS surfactant (30 mM) does not alters the conformation of bovine serum albumin (BSA), and we have observed the formation of SDS micelles coexisting with free protein in solution. The addition of 50 mM of SDS, on the other hand, induces the partial unraveling of the protein, as seen by the analysis of SAXS data via the pearl necklace\'\' model. The effect of adding 3M and 8M urea is, respectively, the partial and total unraveling of BSA, with ensuing aggregation of the protein dependent on the temperature (T > 30°C). The introduction of SDS 6mM promotes further unraveling in proteins that were previously partially unraveled by urea. The resulting effective potential for the interaction between BSA and lysozyme at total concentration of 100mg/ml and 7.0 pH has been obtained from the analysis of SAXS curves. In order to obtain this result we have used a simplified analysis (first order approximation) in which were considered the effective potentials for the interactions between BSA-BSA, lysozyme-lysozyme and lysozyme-BSA. We have varied the BSA:LISO molar ratio up to 1:42. At the studied pH, BSA has a surface residual charge of -11e, and lysozyme has +9e. As we changed the BSA:LISO molar ratio, we have found two regimens for the resulting effective potential: i) up to BSA:LISO 1:2, the effective charge of the system is virtually zero and the resulting potential is attractive; and ii) for BSA:LISO between 1:3 and 1:42 the effective charge increases, and the resulting potential is repulsive. Therefore, both lysozyme and BSA coexist without forming aggregates, by a delicate balance of attractive and repulsive forces.
Vander, Broek Charles William. "Subversion of host cellular processes by the melioidosis pathogen, Burkholderia pseudomallei." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/25402.
Повний текст джерелаAyre, Lorna M. "The adsorption of proteins onto ultrafiltration membranes." Thesis, Loughborough University, 2000. https://dspace.lboro.ac.uk/2134/35236.
Повний текст джерелаSchveitzer, Bianca. "Interação de albumina do soro bovino (BSA) com sais biliares induzidas por surfactantes aniônicos." Florianópolis, SC, 2006. http://repositorio.ufsc.br/xmlui/handle/123456789/88505.
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Neste trabalho, o sal biliar empregado foi o colato de sódio (NaC) para estudar o processo de ligação competitiva entre NaC e o dodecilsulfato de sódio (SDS) sobre a albumina do soro bovino (BSA), em uma solução tampão 0,02 M tris-HCl, em pH 7,5 e a 25 C. A associação entre o NaC e o SDS com a BSA foi monitorado em baixas concentrações de surfactantes onde somente o processo de ligação específica se desenvolve, e também foi acompanhado em altas concentrações de surfactante onde o processo de ligação cooperativa pode ser observado. Em baixas concentrações de surfactante, a metodologia aplicada para monitorar o processo de ligação específica está baseada na análise do efeito da concentração SDS e do NaC e ainda de misturas destes surfactactantes, sobre a intensidade de fluorescência dos resíduos de triptofano da BSA. A metodologia é empregada para se medir a quantidade de monômeros de surfactante distribuidos sobre a molécula de proteína onde a ligação ocorre, como pode ser observado pela supressão da fluorescência dos grupos cromóforos. A análise, baseada na quantidade de molécula de surfactante ligada sobre a proteína, indicou que o SDS é um supressor de fluorescência mais eficiente que o sal biliar. São necessárias 4-6 moléculas de NaC ligadas sobre a proteína para se obter a mesma eficiência na supressão que uma única molécula de SDS. Em altas concentrações de surfactante, os perfis das bandas de emissão de fluorescência são apresentados pela razão de A0/A em função da concentração de surfactante, onde A0 e A representam as áreas das bandas de emissão na ausência e em presença de surfactante, respectivamente. Uma outra metodologia, onde emprega-se gráficos de condutividade elétrica em função da concentração de surfactante, foi usada, para misturas de SDS e NaC, para investigar o processo de associação, e para determinação de parâmetros de ligação, como concentração de agregação crítica, cac, e concentração micelar crítica, cmc.
Ferraz, Caroline Cristina. "Desenvolvimento de uma membrana nanoestruturada à base de poliacrilamida para veiculação de proteínas." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/85/85134/tde-19112013-145148/.
Повний текст джерелаThe use of hydrogels for biomedical purposes has been extensively investigated. Polyacrylamide (PAAM) is widely used due to properties such as hydrophilicity and swelling degree. Pharmaceutical proteins correspond to highly active substances which may be applied for distinct purposes. This work concerns the development of radio-synthesized hydrogel for protein release using papain and bovine serum albumin (BSA) as model proteins. The polymer was solubilized (1% w/v) in water and lyophilized. The proteins were incorporated into the lyophilized polymer and the hydrogels were produced by simultaneous crosslinking and sterilization using gamma radiation at 25 kGy under frozen conditions. The produced systems were characterized in terms of swelling degree, gel fraction, crosslinking density, fluid handling capacity, determination pH at point of polymer zero charge and evaluated according to protein release, bioactivity, cytotoxicity and cell adhesion. The hydrogels developed presented different properties as a function of polymer concentration and the optimized results were found for the samples containing 4-10% polyacrylamide. Protein release was controlled by the electrostatic affinity of acrylic moieties of polymer and proteins. This selection was based on the release of the proteins during the experiment period (up to 50 hours), maintenance of enzyme activity and the nanostructure developed. The system was suitable for protein loading and release and according to the cytotoxic assay and cell adhesion it was also adequate for biomedical purposes and this method was able to generate a matrix to protein release.
Zheng, Yu 1970. "Melt growth of BSO (Bi₁₂SiO₂₀) : critical issues for growth in a micro-g environment." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/9129.
Повний текст джерелаIncludes bibliographical references (leaves 117-122).
Bismuth Silicate, Bi12Si020, is a body-centered cubic oxide exhibiting non-linear optical preperties which make it a target for many applications including optical information processing. This project is to investigate and resolve some critical issues associated with the Bridgman growth of BSO under the microgravity conditions, which can be divided into three phases according to the subject in each phase. (1) The wetting behavior between BSO and the confinement material, platinum, is critical in a Bridgman type of growth (Chap 3). The sessile drop method combined with a drop shape analysis was used to measure and compute the three-phase contact angles and the surface tensions ofBSO melts under varying partial pressure of oxygen (P02). The contact angle changed from 45° to less than 15° as P02 increased from 104 atm to 10-2 atm, however, the surface tension of BSO melts (- 200 mJ/m2) showed no dependence on P02. Based on the partial wetting conditions, platinum confinements for the growth of BSO with and with out gravity were designed. (2) Thermal characteristics in a Bridgman system, specifically the distribution and the stability of the axial and radial thermal profile within the charge, are critical for the establishment of desirable growth environment (Chap 4 ). In this phase of study, the thermal characterization of a modified vertical Bridgman system for growth of BSO were conducted by measuring the axial and radial temperature distribution in a semitransparent dummy charge made of fused silica which has comparable thern1a] and optical properties as BSO. A modified control method, in which two control thermocouples are placed in the gradient zone, was proved effective in stabilizing the axial thermal gradient. Thermal radiation was found to have a significant role in determining the temperature distribution inside the semitransparent charge. Use of a metallic confmement around the charge was able to reduce the influence of the external thermal radiation, and generated the typical transition of isothermal profile from convex to planar, then to concave along the axial direction in the charge. The effect of the remaining thermal radiation through the metallic shield caused the asymmetry of the axial thermal profile and resulted iri a high position ( close to the hot zone) of the planar isotherm. (3) Three ground-based growth experiments were conducted in this phase using the proposed ampoule design and growth conditions (Chap 5). Single crystals with desirable interface morphology were obtained, which confirmed the effectiveness of the thermal characterization. The position of the interface shifted during the growth due to the end effects of a short charge. The solution to this problem is to use a longer charge and/or to place the two control thermocouples closer to each other. Embrittlement of platinum happened when the ampoule was sealed during the growth. To avoid this problem, a dynamic airflow system should be constructed in the VB furnace, and the ampoule should be left open during the growth process. Interesting solid phase inclusions with unknown identities were found in one VBgrown crystal. The origin of such inclusions, as well as their correlation to the growth conditions, is remained as the subjects for the future work. The support of the National Aeronautics and Space Administration is gratefully acknowledged.
by Yu Zheng.
Ph.D.
Santos, Sandra Maria Lopes dos. "Síntese e caracterização de sílicas mesoporosas para adsorção de biomoléculas modelo (BSA, Lisozima e Celulase)." reponame:Repositório Institucional da UFC, 2013. http://www.repositorio.ufc.br/handle/riufc/11738.
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Part of the cost of production of biomolecules with high purity and yield depends on the separation and purification steps used. In these steps, it is usually necessary to use chromatography techniques and one of the factors that influence on its high performance is the development of stationary phases or suitable adsorbents. The present study investigates the adsorption of biomolecules three model (bovine serum albumin - BSA, lysozyme - LYS cellulase and - CEL) in mesoporous silica obtained from tri-block copolymers as agents drivers structure. We report different synthesis procedures aimed at modifying the surface chemistry and promoting textural changes, such as enlargement and/or pores, and the mesoscopic ordering. In the first part of this thesis were synthesized (i) SBA-15 by two different routes, sol-gel and hydrothermal and (ii) SBA-16 by hydrothermal. In the second part, SBA-15 samples with different pore sizes and channel length were s ynthesized using 1,3,5-trimethylbenzene (TMB), heptane and ammonium fluoride (NH4F). TMB was used to increase the pore diameter and heptane combined with NH4F to modify the size of the channels of SBA-15. In the third part of the work, the acidity of SBA-15 was modified by the addition of zirconium with three molar ratios Si/Zr distinct (5, 10 and 15). The adsorption of the three target biomolecules was studied by experiments in stirred tanks. The results indicate that for pure silicas, higher adsorption capacities are obtained when the pH is close to the isoelectric point of biomolecule. Very promising results for were found the adsorption of proteins on silicas with larger pore diameters (above 10 nm), up to hundreds of milligrams per gram of adsorbent. In the case of materials with zirconium, the best results were fou nd for the materials with the lowest amount of said heteroatom (Si/Zr = 15), which have similar texture to the original support. This suggests that there may be a moderate acidity which-enhances the adsorption of the studied biomolecules or excess zirconium atoms lead to steric hindrances causing a decrease in the adsorption capacity of the biomolecule
Parte do custo de produção de biomoléculas com elevada pureza e o rendimento deste processo depende das etapas de separação e purificação utilizadas. Nestas etapas, geralmente é necessário utilizar técnicas de cromatografia e um dos fatores que influenciam no seu elevado desempenho é o desenvolvimento de fases estacionárias ou adsorventes adequados. O presente trabalho investiga a adsorção de três biomoléculas-modelo (albumina de soro bovino - BSA, lisozima - LYS e celulase – CEL) em sílicas mesoporosas obtidas a partir de copolímeros tribloco como agentes direcionadores estruturais. Relatam-se diferentes procedimentos de síntese que visam modificar a química da superfície e promover alterações texturais como, por exemplo, o alargamento e/ou encurtamento dos poros, bem como a sua ordenação mesoscópica. Na primeira parte da tese, foram sintetizadas (i) SBA-15 por duas rotas distintas, sol-gel e hidrotérmica, e (ii) SBA-16 por via hidrotérmica. Na segunda parte, amostras de SBA-15 com diferentes tamanhos de poros e comprimento de canais foram sintetizados utilizando 1,3,5-trimetilbenzeno (TMB), heptano e fluoreto de amônio (NH4F). O TMB foi utilizado para aumentar o diâmetro dos poros e o heptano combinado com NH4F, para modificar o tamanho dos canais da SBA-15. Já na terceira parte do trabalho, a acidez da SBA-15 foi modificada pela adição de zircônio com três razões molares Si/Zr distintas (5, 10 e 15). A adsorção das três biomoléculas-alvo foi estudada por experimentos em tanques agitados. Os resultados indicam que, para sílicas puras, maiores capacidades de adsorção são obtidas quando o pH está próximo ao ponto isoelétrico da biomolécula. Resultados muito promissores foram encontrados para a adsorção de proteínas sobre as sílicas com maior diâmetro de poros (acima de 10 nm), chegando a centenas de miligramas por grama de adsorvente. No caso dos materiais com zircônio, os melhores resultados foram encontrados para os materiais com a menor quantidade do referido heteroátomo (Si/Zr = 15), que apresentam textura similar ao suporte original. Isto sugere que pode haver uma acidez moderada ótima para a adsorção das biomoléculas estudadas ou que o “excesso” de átomos de zircônio leva a impedimentos estéricos que causam uma redução na capacidade de adsorção da biomolécula
Santos, Sandra Maria Lopes dos. "Synthesis and characterization of mesoporous silica for the adsorption of biomolecules model (BSA, lysozyme and cellulase)." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14021.
Повний текст джерелаConselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
Parte do custo de produÃÃo de biomolÃculas com elevada pureza e o rendimento deste processo depende das etapas de separaÃÃo e purificaÃÃo utilizadas. Nestas etapas, geralmente à necessÃrio utilizar tÃcnicas de cromatografia e um dos fatores que influenciam no seu elevado desempenho à o desenvolvimento de fases estacionÃrias ou adsorventes adequados. O presente trabalho investiga a adsorÃÃo de trÃs biomolÃculas-modelo (albumina de soro bovino - BSA, lisozima - LYS e celulase â CEL) em sÃlicas mesoporosas obtidas a partir de copolÃmeros tribloco como agentes direcionadores estruturais. Relatam-se diferentes procedimentos de sÃntese que visam modificar a quÃmica da superfÃcie e promover alteraÃÃes texturais como, por exemplo, o alargamento e/ou encurtamento dos poros, bem como a sua ordenaÃÃo mesoscÃpica. Na primeira parte da tese, foram sintetizadas (i) SBA-15 por duas rotas distintas, sol-gel e hidrotÃrmica, e (ii) SBA-16 por via hidrotÃrmica. Na segunda parte, amostras de SBA-15 com diferentes tamanhos de poros e comprimento de canais foram sintetizados utilizando 1,3,5-trimetilbenzeno (TMB), heptano e fluoreto de amÃnio (NH4F). O TMB foi utilizado para aumentar o diÃmetro dos poros e o heptano combinado com NH4F, para modificar o tamanho dos canais da SBA-15. Jà na terceira parte do trabalho, a acidez da SBA-15 foi modificada pela adiÃÃo de zircÃnio com trÃs razÃes molares Si/Zr distintas (5, 10 e 15). A adsorÃÃo das trÃs biomolÃculas-alvo foi estudada por experimentos em tanques agitados. Os resultados indicam que, para sÃlicas puras, maiores capacidades de adsorÃÃo sÃo obtidas quando o pH està prÃximo ao ponto isoelÃtrico da biomolÃcula. Resultados muito promissores foram encontrados para a adsorÃÃo de proteÃnas sobre as sÃlicas com maior diÃmetro de poros (acima de 10 nm), chegando a centenas de miligramas por grama de adsorvente. No caso dos materiais com zircÃnio, os melhores resultados foram encontrados para os materiais com a menor quantidade do referido heteroÃtomo (Si/Zr = 15), que apresentam textura similar ao suporte original. Isto sugere que pode haver uma acidez moderada Ãtima para a adsorÃÃo das biomolÃculas estudadas ou que o âexcessoâ de Ãtomos de zircÃnio leva a impedimentos estÃricos que causam uma reduÃÃo na capacidade de adsorÃÃo da biomolÃcula.
Part of the cost of production of biomolecules with high purity and yield depends on the separation and purification steps used. In these steps, it is usually necessary to use chromatography techniques and one of the factors that influence on its high performance is the development of stationary phases or suitable adsorbents. The present study investigates the adsorption of biomolecules three model (bovine serum albumin - BSA, lysozyme - LYS cellulase and - CEL) in mesoporous silica obtained from tri-block copolymers as agents drivers structure. We report different synthesis procedures aimed at modifying the surface chemistry and promoting textural changes, such as enlargement and/or pores, and the mesoscopic ordering. In the first part of this thesis were synthesized (i) SBA-15 by two different routes, sol-gel and hydrothermal and (ii) SBA-16 by hydrothermal. In the second part, SBA-15 samples with different pore sizes and channel length were s ynthesized using 1,3,5-trimethylbenzene (TMB), heptane and ammonium fluoride (NH4F). TMB was used to increase the pore diameter and heptane combined with NH4F to modify the size of the channels of SBA-15. In the third part of the work, the acidity of SBA-15 was modified by the addition of zirconium with three molar ratios Si/Zr distinct (5, 10 and 15). The adsorption of the three target biomolecules was studied by experiments in stirred tanks. The results indicate that for pure silicas, higher adsorption capacities are obtained when the pH is close to the isoelectric point of biomolecule. Very promising results for were found the adsorption of proteins on silicas with larger pore diameters (above 10 nm), up to hundreds of milligrams per gram of adsorbent. In the case of materials with zirconium, the best results were fou nd for the materials with the lowest amount of said heteroatom (Si/Zr = 15), which have similar texture to the original support. This suggests that there may be a moderate acidity which-enhances the adsorption of the studied biomolecules or excess zirconium atoms lead to steric hindrances causing a decrease in the adsorption capacity of the biomolecule
Boury, Frank. "Influence des propriétés de surface de microsphères de poly(alpha-hydroxyacides) sur l'adsorption de la BSA." Paris 11, 1995. http://www.theses.fr/1995PA114801.
Повний текст джерелаAntonio, Ocleia Gomes de Araujo. "Impactos da implantação do sistema de bônus em equipes de trabalho: um estudo de caso na UN-BSOL Petrobrás na Amazônia." Universidade Federal de Minas Gerais, 2004. http://hdl.handle.net/1843/BUBD-9BFK7P.
Повний текст джерелаOs modelos de recompensar os empregados vêm sendo ampliados e adaptados ao contexto em que vive hoje o mundo empresarial, tendo em vista as novas formas de gestão de pessoas. Os sistemas de bonificações têm surgido como mais uma alternativa para premiação e retenção de talentos nas organizações. É um tema polêmico e vem sendo bastante discutido na área de administração de pessoal. Na UN-BSOL, o bônus foi implantado no ano de 2000 e causou alguns conflitos na organização, pois o modelo de gestão necessita que seus empregados trabalhem em equipes, compartilhando idéias, resultados, exercitando a cooperação, mas o bônus premia individualmente, quando os empregados esperam um reconhecimento em equipes. Nesse sentido, o presente trabalho teve como principal objetivo descrever e demonstrar impactos da implantação do sistema de bônus nas equipes de trabalho na percepção dos empregados da UN-BSOL. Foi realizado um estudo de caso e, por meio de entrevistas a 31 empregados, em um universo de 352, verificou-se que a adoção do sistema de bônus gerou impactos positivos e negativos no trabalho em equipe. Como impactos positivos, os empregados responderam que o bônus favorece um maior envolvimento das pessoas com os trabalhos, estimula a criatividade e serve para reconhecer o serviço de alguns empregados. Como impactos negativos, o bônus gerou, principalmente, competição entre os empregados e descontentamento pela forma como foi utilizado, destacando-se, dentre outros itens, principalmente a falta de critérios justos. Recomenda-se que, ao se aplicar um modelo de reconhecimento de empregados, dentre outros aspectos, seja levada em consideração a subjetividade no processo, os aspectos culturais da organização, bem como a sensibilização dos empregados para o modelo a ser aplicado. Os resultados da pesquisa podem ser considerados valiosos, não somente por agregarem valor ao conhecimento acadêmico sobre o tema, mas também por se apresentarem como contribuições à empresa pesquisada, relativamente à condução dos processos de reconhecimento de empregados.
Soares, de Vasconcelos Gabriel. "Segmentação de pupila utilizando redes neurais batch - SOM." Universidade Federal de Pernambuco, 2011. https://repositorio.ufpe.br/handle/123456789/2729.
Повний текст джерелаOs recentes avanços da tecnologia de informação e o crescimento dos requisitos de segurança têm impulsionado o aprimoramento dos métodos de identificação pessoal. Os métodos de identificação tradicionais baseados em posse (cartões, chaves, entre outros objetos) ou conhecimento (login e senha, por exemplo) apresentam alguns incovenientes, considerando que os objetos podem ser perdidos, roubados ou falsificados e que nomes de usuários e senhas podem ser esquecidos ou até adivinhados. O desenvolvimento dos métodos biométricos de identificação pessoal surgem como uma alternativa para superar estas limitações. Nestes métodos, a associação da identidade passa a ser baseada em características próprias e inerentes a cada pessoa. Estas características representam o que indivíduo é ou como ele realiza alguma ação, e não um objeto que o indivíduo possui ou algo que ele precise lembrar. Desta maneira, as características biométricas não podem ser esquecidas ou compartilhadas e dificilmente são copiadas ou modificadas. Dentre todos os métodos biométricos, os sistemas baseados no reconhecimento de íris vêm ganhando destaque em virtude de ser considerado como uma das modalidades biométricas mais precisas. Uma de suas etapas mais críticas é a etapa de segmentação, na qual, a região da íris é localizada e extraída a partir de uma imagem do olho previamente coletada, para que os modelos biométricos posteriormente gerados contenham apenas informações de íris. Uma representação errônea da região de íris corromperá o modelo biométrico, resultando em baixas taxas de reconhecimento. Essa etapa é, geralmente, subdividida em duas: segmentação de pupila e segmentação de íris, assumindo que a partir da segmentação da pupila, a segmentação da íris torna-se menos complexa, devido, em parte, à área de busca pela íris ser reduzida aos arredores da pupila. Usualmente, as técnicas de segmentação de pupila são baseadas na detecção de círculos, porém, é comum a pupila apresentar-se com um formato irregular em imagens do olho, principalmente, devido a problemas durante a etapa de aquisição da imagem. Nesta dissertação é proposta uma nova técnica baseada na utilização de uma rede neural batch-SOM (BSOM) modificada para o problema de segmentação de pupila que, diferente de outras técnicas, pode assumir qualquer formato, ajustando-se de maneira mais precisa às fronteiras da pupila. Nesta dissertação, também foram sugeridos um método, baseado no algoritmo K-means, para inicializar a rede neural e um método de ajuste do contorno obtido pela rede BSOM. Os resultados finais alcançados mostraram-se excelentes para as bases CasiaIris-V3 Interval, CasiaIris-V4 Syn e MMU1
Allmann, Bernhard. "Lernen im Fernunterricht eine Untersuchung zur Akzeptanz von fernunterrichtsrelevanten Grössen am Beispiel der Fernlehrgänge der BSA-Akademie /." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974384747.
Повний текст джерелаRichter, Doreen [Verfasser]. "Occurrence and fate of sulfonamides (p-TSA, o-TSA, BSA) in an urban water cycle / Doreen Richter." Berlin : Freie Universität Berlin, 2008. http://d-nb.info/1023023725/34.
Повний текст джерелаDiop, Bernand-Mantel Dior. "Films polymères minces à base de méthacrylate de glycidyle pour l'élaboration d'interfaces immunoréceptrices : étude par résonance de plasmon de surface." Phd thesis, Université Paris-Est, 2010. http://tel.archives-ouvertes.fr/tel-00610387.
Повний текст джерелаANJOS, Jorge Luiz Vieira dos. "Interações da albumina de soro bovino com surfactantes e efeitos de antioxidantes sobre a oxidação de lipoproteínas de baixa densidade induzida por íons de cobre." Universidade Federal de Goiás, 2012. http://repositorio.bc.ufg.br/tede/handle/tde/1236.
Повний текст джерелаHuman plasma contains primarily large proteins, ranging in composition and concentration as the individual's physiological state. Among these proteins, albumin and low density lipoprotein (LDL) have been widely studied. The albumin (the most abundant protein in blood plasma) is responsible for important functions in the human body due to its excellent ability to bind and transport small molecules. In turn, the LDL (responsible for transporting cholesterol to the cells) in its oxidized form is directly associated with atherosclerosis, the main cause of cardiovascular disease. In the first part of this work, the interaction of bovine serum albumin (BSA) with the ionic surfactants sodium dodecylsulfate (SDS), cetyltrimethylammonium chloride (CTAC) and N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (HPS) was studied by electron paramagnetic resonance (EPR) spectroscopy of spin label covalently bound to the single free thiol group of the protein. In the second part was studied the oxidation of human LDL by copper ions and also the antioxidant potential of polyphenols resveratrol, (+)-catechin and quercetin, using the EPR of a spin label, derived from stearic acid (5-DSA), and the method malondialdehyde content (MDA). Part I: The dynamics of the BSA and the thermodynamic parameters for transferring the nitroxide side chain from the more motionally restricted to the less restricted component were monitored through EPR spectra simulation. Whereas SDS and CTAC showed similar increases in the dynamics of the protein backbone for all concentrations used, HPS presented a smaller effect at concentrations above 1.5mM. At 10mM of surfactants and 0.15 mM BSA, the standard Gibbs free energy change was consistent with protein backbone conformations more expanded and exposed to the solvent, but with a less pronounced effect for HPS. In the presence of the surfactants, the enthalpy change, related to the energy required to dissociate the nitroxide side chain from the protein, was greater, suggesting a lower water activity. The nitroxide side chain also detected a higher viscosity environment in the vicinity of the Mal-5 induced by the addition of the surfactants. The results suggest that the surfactant-BSA interaction, at higher surfactant concentration, is affected by the affinities of the surfactant to its own micelles and micelle-like aggregates. Complementary DLS (Dynamic Light Scattering) data suggests that the temperature induced changes monitored by the Mal-5 reflects local changes in the vicinity of Cys-34 BSA residue. Part II: The oxidative process induced by copper ions results in lipid peroxidation of LDL (evidenced by high concentration of MDA) could also be monitored by the decrease in the dynamics of 5-DSA, reflected in increased spectral parameter 2A//. The oxidation of LDL resulted in increased energy barrier that the spin labels must overcome to achieve higher degrees of motion. All polyphenols studied were able to protect LDL completely against oxidation for concentrations from 30 M, whereas the protection provided by the Butylated hydroxytoluene (BHT) occurred only partially. This result, based on data from the literature, was attributed to the ability of polyphenols act as scavenger and chelating agents, while the BHT acts just like scavenger due the presence of only a single hydroxyl group in its molecule.
O plasma humano contém principalmente grandes proteínas, com variação na composição e concentração conforme o estado fisiológico do individuo. Entre essas proteínas, a albumina e a lipoproteína de baixa densidade (LDL) têm sido amplamente estudadas. A albumina (proteína mais abundante do plasma sanguíneo) é a responsável por importantes funções no organismo humano devido a sua excelente capacidade de se ligar e transportar pequenas moléculas. Por sua vez, a LDL (responsável pelo transporte de colesterol para as células) em sua forma oxidada está diretamente associada à aterosclerose, principal causa de doenças cardiovasculares. Na primeira parte deste trabalho, a interação da albumina de soro bovino (BSA) com os surfactantes iônicos dodecil sulfato de sódio (SDS), cloreto de cetiltrimetilamônio (CTAC) e N-hexadecil-N,N, dimetil-3-3amônio-1-propano sulfonato (HPS) foi estudada através da espectroscopia de ressonância paramagnética eletrônica (RPE) do marcador de spin Mal-5 ligado covalentemente na cadeia lateral do resíduo Cys-34 da BSA. Na segunda parte foi estudada a oxidação da LDL humana por íons de cobre e também o potencial antioxidante dos polifenóis resveratrol, (+)-catequina e quercetina, usando a RPE de um marcador de spin derivado do ácido esteárico (5-DSA) e o método de formação de malondialdeído (MDA). Parte I: A dinâmica da BSA e os parâmetros termodinâmicos para transferir a cadeia lateral do nitróxido da componente de movimento mais restrito para a componente menos restrita foram monitorados através da simulação dos espectros de RPE. Enquanto o SDS e o CTAC mostraram efeitos similares na dinâmica da proteína para todas as concentrações usadas, o HPS apresentou menor efeito em concentrações acima de 1,5 mM. Em 10 mM de surfactantes e 0,15 mM de BSA, a variação da energia livre padrão de Gibbs foi consistente com a conformação da cadeia proteica mais expandida e mais exposta ao solvente, mas com um efeito menos pronunciado para o HPS. Na presença dos surfactantes, a variação de entalpia, relacionada a energia necessária para dissociar a cadeia lateral do nitróxido da proteína, foi grande, sugerindo uma menor atividade da água. A cadeia lateral do nitróxido também detectou um ambiente com maior viscosidade nas vizinhanças do Mal-5 induzida pela adição dos surfactantes. Os resultados sugerem que a interação surfactante-BSA, em altas concentrações, é afetada pela afinidade do surfactante por suas próprias micelas e agregados micelares incorporados na proteína. Complementarmente, dados obtidos com DLS (Dynamic Light Scattering) sugerem que as mudanças induzidas pela temperatura que são monitoradas pelo Mal-5 são mudanças locais na vizinhança do único resíduo Cys-34 da BSA. Parte II: O processo oxidativo induzido pelos íons de cobre resulta na peroxidação dos lipídios da LDL (evidenciado pela elevação da concentração de MDA) também pôde ser monitorado pela diminuição na dinâmica do marcador de spin 5-DSA refletida no aumento do parâmetro espectral 2A//. A oxidação da LDL acarretou no aumento da barreira da energia que os marcadores de spin precisam superar para alcançar graus superiores de movimento. Todos os polifenóis estudados foram capazes de proteger completamente a LDL contra a oxidação em concentrações a partir de 30 M, enquanto que a proteção fornecida pelo butil-hidroxi-tolueno (BHT) se deu apenas parcialmente. Este resultado, baseado em dados da literatura, foi atribuído à capacidade dos polifenóis atuarem tanto como scavenger quanto como quelantes, ao passo que o BHT é capaz de atuar apenas como scavenger devido à presença de apenas uma única hidroxila em sua molécula.
Fernandes, Denise de Castro. "Efeitos do tempol sobre a interação entre peroxinitrito/CO2 com albumina e macrófagos: inibição da nitração de tirosinas e da oxidação de cisteínas e amplificação da nitrosação de cisteínas." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-17112014-160500/.
Повний текст джерелаTempol has been shown to protect animals from oxidative stress conditions. Tempol\'s protective mechanisms against reactive oxygen species have been extensively studied but its interactions with reactive nitrogen species remain little explored. Recently, we proposed that tempol diverts peroxynitrite/CO2 mediated phenol nitration to nitrosation by reacting with CO3•‾ to produce tempol oxamonium cation that oxidizes peroxynitrite to O2 and •NO. The latter produces a nitrosating species by reacting with peroxynitrite-derived •NO2 [Bonini et al. (2002) Chem. Res. Toxicol. 15: 506]. To examine wether this mechanism operates in biological environments, we studied the effects of tempol on peroxynitrite/CO2 reactivity towards a protein, BSA, and cells, macrophages. Tempol\'s effects were dependent on its own and BSA-cys concentration. Although not a true catalyst, it inhibited BSA-cys oxydation (20-50%) and BSA-tyr nitration (70-90%) while increasing BSA-cys nitrosation (200-400%). In the case of macrophages treated with peroxynitrite/CO2, tempol also inhibited protein-tyr nitration (90%) and increased protein-cys nitrosation (300%). Then, under physiological conditions, a substoichiometric amount of tempol is able to divert peroxynitrite-derived radicais reactivity from protein-cys oxidation and protein-tyr nitration to protein-cys nitrosation. This may be the mechanism by wich tempol inhibits injury in inflammatory conditions.
Rivera-Jiménez, Hernando Javier. "Desenvolvimento de marcadores moleculares espécie-específicos para a identificação de Eucalyptus." Botucatu, 2016. http://hdl.handle.net/11449/143468.
Повний текст джерелаResumo: The forest-breeding program in Brazil has the general objective of providing most adapted plants to different environments for various Brazilian regions, for fulfilling timber demands meant for multiple uses in the country. One of the main problems found in different forest breeding programs are the difficulty to identify the different species and hybrids. The use of molecular biology techniques in plant breeding programs is found very effective in the optimization of the time and the direction of these programs, particularly among those plants of the same subgenus. The process of selection and hybrid plants selected for planting in most cases; significantly increase the gain in terms of production and adaptability. The use of molecular markers to characterize the molecular variability of forest species has revolutionized genetic analysis in recent years. The bulk segregant analysis (BSA) is a technique used to identify molecular markers linked to monogenic, dominant or recessive characters. BSA technique in combination with Amplified Fragment Length Polymorphisms (AFLP) technique is an efficient methodology for the detection of polymorphism from genomic restriction fragments through PCR amplification; which helps in analyzing large number of loci for testing without the need for previous information of their sequence in respect to their dominance and reproducibility. The most recent and promising applications of molecular biological methods for the detection of small DNA fra... (Resumo completo, clicar acesso eletrônico abaixo)
Doutor
Dávila, Rodríguez María José. "Compostos carbonílicos heterobimetálicos de Ru(II)/Fe(II) : propriedades citotóxicas e estudo da interação com BSA e DNA." Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/8032.
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Ruthenium and iron-based drugs are an option for the development of improved chemotherapeutic agents for cancer treatment with lower toxicity. Thereby, this work present the in vitro cytotoxic activity and in vitro interaction studies of Calf-Thymus DNA (CT-DNA) and bovine serum albumin (BSA) with the synthetized carbonyl heterobimetallic compounds of Ru(II)/Fe(II) with general formula ct-[RuCl(CO)(N-N)(dppf)]PF6, with N-N = 1,10-phenantrholine (phen, 1); dipyrido[3,2-f:2′,3′-h]quinoxaline (dpq, 2); dipyrido[3,2-a:2',3'-c]phenazine (dppz, 3); dipyrido[3,2-f:2′,3′-h]quinoxalino[2,3-b]quinoxaline (dpqQX, 4) and dppf = 1,1’-bis(diphenylphosphino)ferrocene. Spectroscopy (IR, UV-vis and 1H-13C{1H}- and 31P{1H}-NMR) and voltammetry techniques along with elemental analysis were employed for the characterization of the complexes. Spectrofluorimetric titrations shows strong and spontaneous interactions of 1–3 with BSA through a static quenching mechanism resulting in binding constants in the order of 104 - 106 L·mol-1 at 310 K. Viscosity measurements and circular dichroism spectra prompts interactions of 1–4 with CT-DNA via non-classical intercalations or by an electrostatic pathway. MTT assays in cell lines MDA-MB-231 and V79-4 revealed IC50 values at range of 0,19 – 1,11 µmol·L-1 and 1,29 – 3,85 µmol·L-1 respectively, after 48 h of exposure to 1–4. However, stability studies of solutions 1 mmol·L-1 of 1–4 in DMSO shows their rapid reaction with this solvent.
Fármacos baseados em complexos heterobimetálicos de rutênio e ferro são alternativas para o desenvolvimento de melhores e menos tóxicos agentes quimioterápicos para o tratamento do câncer. Assim, este trabalho apresenta a atividade citotóxica in vitro e os estudos de interação in vitro com DNA de timo de bezerro (CT-DNA) e albumina de soro bovino (BSA) com os compostos carbonílicos heterobimetálicos de Ru(II)/Fe(II) de fórmula geral ct-[RuCl(CO)(N-N)(dppf)]PF6, com N-N = 1,10-fenantrolina (fen, 1); dipirido[3,2-f:2′,3′-h]quinoxalina (dpq, 2); dipirido[3,2-a:2',3'-c]fenazina (dppz, 3); dipirido[3,2-f:2′,3′-h] quinoxalino[2,3-b]quinoxalina (dpqQX, 4) e dppf = 1,1'-bis(difenilfosfino)ferroceno. Foram empregadas técnicas de espectroscopia (IV, UV-vis, e RMN de 1H, 13C{1H} e 31P{1 H}) e voltametria juntamente com a análise elementar para a caracterização dos compostos sintetizados. Titulações espectrofluorométricas mostraram interações fortes e espontâneas de 1–3 com a BSA através de um mecanismo de supressão da fluorescência estático, apresentando constantes de ligação na ordem de 104 – 106 L·mol-1 a 310 K. Medidas de viscosidade e espectros de dicroísmo circular, indicam interações de 1–4 com o CT-DNA via intercalações não clássicas ou por via eletrostática. Ensaios MTT nas linhagens celulares MDA-MB-231 e V79-4, revelaram valores de IC50 na faixa de 0,19 – 1,11 μmol·L -1 e 1,29 – 3,85 μmol·L-1 respectivamente, com 48h de exposição aos compostos 1–4. No entanto, estudos de estabilidade de soluções 1 mmol·L-1 de 1–4 em DMSO, mostraram sua rápida reação com este solvente.
Landers, Alexis [Verfasser], Rita A. [Gutachter] Depprich, and Christoph [Gutachter] Suschek. "Freisetzungskinetik des Modellproteins FITC-BSA aus verschieden polymerbeschichteten bovinen Kollagenmatrices / Alexis Landers ; Gutachter: Rita A. Depprich, Christoph Suschek." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2019. http://d-nb.info/1175624837/34.
Повний текст джерелаBorba, Laise Costa. "Síntese de líquidos iônicos anfifílicos derivados de Oxa (tia) zolidinas e estudo da interação com BSA e lipossomas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2018. http://hdl.handle.net/10183/180654.
Повний текст джерелаIonic liquids can be considered as amphiphilic structures and have similarities with the surfactants, once they generally have an apolar alkyl chain and a polar moiety, such as the imidazolium cation. Such structures, due to this amphiphilic character, may interact with liposome structures or even with biomolecules, such as BSA. In this work, three new ionic liquids containing chiral systems derived from naturally occurring amino acids, such as L-Cysteine, L-Serine and L-Threonine were synthesized, with yields ranging from 52 to 66%. They were fully characterized both from the structural point of view, and by its photophysical properties. As methodological procedures, amino acid cyclocondensation, Steglich esterification and alkylation were used. In order to investigate and confirm these structures, magnetic resonance studies of 1H and 13C, infrared, as well as absorption spectroscopies in the UV-Vis region and fluorescence emission were used. At last, these new ionic liquids were tested with liposomes and BSA, and the results showed good interaction with these biomolecules.
Marchais, Hervé. "Preparation et caracterisation de microspheres de pla25ga50 chargees en bsa : etude de leur absorption intestinale chez le rat." Angers, 1994. http://www.theses.fr/1994ANGE0506.
Повний текст джерелаMcKinley, Laura Ellen. "Neutron reflectivity studies of bacterial membranes, peptides and proteins." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28874.
Повний текст джерелаBlackard, Glynis D. "Examining Bachelor of Science in Nursing Accelerated (BSNA) Program Outcomes within a Traditional Faculty Workload Model versus a Clock Hour Faculty Workload Mod." Thesis, William Carey University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10192207.
Повний текст джерелаLiterature evaluating the impact of faculty workload on student outcomes of graduation and first-time pass for the National Council on Licensure Examination (NCLEX-RN®) is limited. Current models of workload are not specific and do not define the number of hours the faculty spends doing the work. In this day of decreased funding and increased requirement for quality in academia, it is important for nurse educators to understand how quality program outcomes, finances, and faculty workload are connected and impact student involvement.
The purpose of this study was to examine the relationship between the program outcomes of graduation and first-time pass on the National Council on Licensure Examination – Registered Nurse (NCLEX-RN®) for Bachelor of Science Nursing Accelerated (BSNA) student graduates in programs that used a traditional faculty workload model versus a clock hour faculty workload model.
The study, though limited, did begin to evaluate the impact of faculty workload on program outcomes. The intent and goals of the study were to research traditional workload models and alternative models proposed to address the cost of education and establish a baseline of how the models may impact program outcomes. In addition, the intent included evaluating how future changes may ultimately impact the student. Implications of the study included the consideration by faculty, school of nursing administration, and university administration of how faculty workload impacts the classroom and practice environment. Recommendations for further research conclude the study.
Santos, Michelly Christine dos. "Nanopartículas magnéticas funcionalizadas com bicamada de ácido láurico: caracterização, associação de moléculas bioativas e estudo de interação com BSA." Universidade Federal de Goiás, 2012. http://repositorio.bc.ufg.br/tede/handle/tede/8867.
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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Magnetic nanoparticles with two different chemical compositions, magnetite and zinc doped manganese ferrite were synthesized, functionalized with lauric acid bilayers and dispersed into water yielding stable aqueous colloidal suspensions at physiological pH. Bioactive molecules of different sizes, Amphotericin B and curcumin, were associated to the lauric acid bilayers of the suspended nanoparticles. X-ray diffraction analyses reveled a diffraction pattern characteristic of the cubic spinel crystalline phase for both samples. The nanoparticles average size estimated by Debye-Scherrer’s formula was 9 nm for zinc doped manganese ferrite and 8 nm for magnetite. The hysteresis curves showed that the nanoparticles are super paramagnetic at room temperature with saturation magnetization of 44.2 emug-1 for zinc doped manganese ferrite and 51.5 emug-1 for magnetite. The IR analysis showed the presence of lauric acid associated to the nanoparticles, and the TG curves showed a higher amount of lauric acid bonded to zinc doped manganese ferrite. The hydrodynamic diameters of the nanoparticles indicate that they are dispersed into the suspension as aggregates, and that the association of bioactive molecules did not affect the hydrodynamic sizes of the aggregates. The IR spectra confirmed the presence of bioactive molecules in the formulations and the UV-VIS spectra showed that these molecules are solubilized into the lauric acid bilayers in their monomeric forms. The functionalized nanoparticles containing the bioactive molecules present less negative zeta potential than the lauric acid functionalized nanoparticles, indicating that the bioactive molecules interact with the medium of the suspensions. The evaluation of aggregation of the nanoparticles in cell culture medium showed that the nanoparticles aggregation is significantly reduced by the addition of proteins (FBS and BSA) in cell culture medium. The study of interaction of the nanoparticles with bovine serum albumin before and after the association with the bioactive molecules showed that these systems present great affinity for BSA, with binding constants (Kb) in the range of 105 -106M-1. These results suggest that the nanoparticles functionalized with lauric acid bilayers, pure or associated to amphotericin B and curcumin, can be bioconjugated to proteins producing formulations with higher colloidal stability in biological media.
Nanopartículas magnéticas com duas diferentes composições químicas, magnetita e ferrita de manganês dopada com zinco, foram sintetizadas, funcionalizadas com bicamadas de ácido láurico e dispersas em meio aquoso produzindo suspensões coloidais estáveis em pH fisiológico. Posteriormente, foram associadas moléculas bioativas de tamanhos diferentes, Anfotericina B e curcumina, nas bicamadas de ácido láurico funcionalizadas nos dois tipos de nanopartículas em suspensão. A caracterização estrutural e composicional das nanopartículas foi realizada por difração de raios X e análise química dos teores de íons metálicos. Os difratogramas mostraram um padrão de difração referente à formação de material constituído por uma única fase cristalina cúbica do tipo espinélio. Os tamanhos médios estimados pela fórmula de Debye-Scherrer foram 9 e 8 nm para as nanopartículas de ferrita de manganês dopadas com zinco e de magnetita, respectivamente. Os teores dos íons metálicos indicaram um pequeno desvio na estequiometria final dos sólidos em relação à estequiometria inicial. As técnicas utilizadas para caracterizar as nanopartículas funcionalizadas antes e após a associação da Anfotericina B e da curcumina foram IV, UV-VIS, TG, VSM e ainda foram realizadas medidas de potencial zeta e de diâmetro hidrodinâmico. As curvas de histerese indicaram a formação de nanopartículas superparamagnéticas à temperatura ambiente com magnetização de saturação de 44,2 emug-1 para a ferrita de manganês dopada com zinco e 51,6 emug-1 para a magnetita. As análises de IV mostraram que as nanopartículas foram funcionalizadas com ácido láurico e as curvas de TG indicaram que as nanopartículas de ferrita de manganês dopadas com zinco contêm maior quantidade de ácido láurico do que as nanopartículas de magnetita. Os valores de diâmetros hidrodinâmicos das nanopartículas indicaram que as mesmas estão dispersas na suspensão na forma de agregados e que a associação das moléculas bioativas não influenciou nos tamanhos hidrodinâmicos dos agregados. Os espectros de IV comprovaram a presença das moléculas bioativas nas formulações e a análise dos espectros de UV-VIS mostrou que as moléculas bioativas estão solubilizadas nas bicamadas de ácido láurico em suas formas monoméricas. As nanopartículas funcionalizadas associadas às moléculas bioativas apresentaram potencial zeta menos negativo que as nanopartículas funcionalizadas, indicando que as moléculas bioativas interagem com o meio das suspensões. O estudo de agregação de nanopartículas em meio de cultura celular mostrou que a adição de proteínas contribui significativamente para a diminuição da agregação das nanopartículas neste meio. O estudo de interação entre a albumina de soro bovino (BSA) e as nanopartículas funcionalizadas antes e após a associação das moléculas bioativas mostrou que os sistemas têm grande afinidade com a BSA, com constantes de ligação (Kb) da ordem de 105–106 M-1. Esses resultados sugerem que as nanopartículas funcionalizadas com bicamadas de ácido láurico, puras ou associadas à Anfotericina B e à curcumina podem ser bioconjugadas a essa proteína produzindo formulações com maior estabilidade coloidal em meios biológicos.
Neto, Diógenes de Sousa. "Interações da porfirina aniônica meso-tetrakis-4-fenilsulfonato (TPPS4) e da albumina de soro bovino (BSA) com surfactantes catiônicos." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-02042015-114519/.
Повний текст джерелаElectron paramagnetic resonance (EPR) and small angle X-ray scattering (SAXS) were used to investigate the interaction of the meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS4) with simple biological membrane models. In the present work, cationic cethyltrimethylammonium chloride (CTAC) micelles were used as mimetic models. RPE and SAXS experiments were performed at room temperature and at pHs 4.0 and 9.0 in order to evaluate whether the protonation state of the TPPS4 affects its interaction with the cationic micelle. EPR analysis showed a similar behavior for both spin labels 5-DSA and 16-DSA, i.e., the addition of porphyrin to the cationic micelles is followed by a reduction of mobility state for both spin labels, mainly at higher porphyrin concentrations. This behavior has been associated to the micellar packing effect, which seems to be supported by SAXS data. The polarity monitored by the spin label 5-DSA was practically the same in the whole porphyrin concentration range and pH values, suggesting that the protonation state of porphyrin did not contribute significantly for its interaction with cationic micelles. On the other hand, the spin label 16-DSA senses a slightly more hydrophobic environment as a function of porphyrin concentration, especially at pH 4.0. These findings indicate that the interaction of porphyrin also occurs at the hydrophobic core of cationic micelles. Indeed, the data obtained from the best fittings for SAXS curves allowed to conclude that the incorporation of porphyrin by the micelles is associated essentially to a shrinking of the paraffinic shortest semi-axis (R par parameter). Nevertheless, the polar shell thickness (σpol) and the electron density (Ρpol) parameters were practically unaltered in the whole porphyrin concentration range. The best-fit SAXS curves were achieved assuming for CTAC micelles a prolate ellipsoidal shape, where the axial ratio (v value) did not exhibit significant changes over the range 2-10 mM of TPPS4 and studied pH values. EPR technique was also used to monitor the interaction of bovine serum albumin (BSA) with cationic cethyltrimethylammonium chloride (CTAC) at pH 7.0. Spinlabeled derivatives of stearic acids (5-DSA and 16-DSA) bound to high-affinity binding sites of BSA revealed that in the presence of surfactant the EPR spectra are composed of two label populations. Spectral analysis was performed using the nonlinear leastsquares (NLSL) simulation program, which allows one to obtain the rotational diffusion rate and the contribution of each component in the EPR spectra as well as to evaluate ix environment polarity where the nitroxides are localized. The values of rotational correlation time, τ, indicated that component 1 displays a more restricted mobility behavior due to spin labels contacting the protein; the less immobilized component 2 arises from label localization in the bulk of micelles. For 5-DSA, a significant immobilization of probes remains even at higher surfactant concentrations, which is consistent with its higher binding constant as compared to 16-DSA. The increase of surfactant concentration leads to the increase in motional levels of component 1 followed by a reduction of this fraction of spin labels
Annarelli, Céline Claire. "Etude de l'adsorption des protéines à la surface de biomatériaux : nouvelles méthodes de classification des interactions BSA-matériaux prothétiques." Lyon 1, 1998. http://www.theses.fr/1998LYO10016.
Повний текст джерелаNyashanu, Mathew. "Beliefs and perceptions in the construction of HIV stigma and sexual health seeking behaviour among Black Sub-Sahara African (BSSA) communities in Birmingham, UK." Thesis, University of Wolverhampton, 2017. http://hdl.handle.net/2436/621154.
Повний текст джерелаLechelon, Mathias. "Long-range electrodynamic interactions among biomolecules." Thesis, Aix-Marseille, 2017. http://www.theses.fr/2017AIXM0469/document.
Повний текст джерелаThe study of living organisms, biology, extends over many fields and in particular, applies to understanding the functioning of living beings. The most complex organisms, such as human beings, have several levels of organization: they are made up successively of organs, tissues, cells, and biomolecules. There are several types of biomolecules including proteins, which are like tiny tools that allow cells to live and interact with their environment. To do this, proteins must come into contact with each other in a very precise and determined way. This thesis tests the existence of long-range electrodynamic forces which would allow them to interact in a rapid and guided way, by studying the absorption or emission of this type of wave by proteins, then the diffusion of these proteins in solution to observe their behavior
Brandani, Giovanni Bruno. "Molecular dynamics simulations of protein adsorption at interfaces." Thesis, University of Edinburgh, 2016. http://hdl.handle.net/1842/20415.
Повний текст джерелаAndersson, Tobias, and Mattias Martinson. "Licenshantering : - Är företag medvetna om sin licenshanteringssituation?" Thesis, University of Kalmar, School of Communication and Design, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-1940.
Повний текст джерелаI ett samhälle där upphovsrätten och immaterialrätten blir allt mer uppmärksammad är det viktigt för företag att vara medvetna om sitt innehav av licenser för mjukvaror. I detta arbete kommer företags medvetenhet om deras licenshanteringssituation att undersökas. I den här rapporten kommer problemställningen att undersökas med hjälp av intervjuer med företag. Dessa intervjuer kommer att ske via telefon och per e-post. Ett liknande arbete har tidigare inte gjorts och därför känns det relevant samt intressant att göra denna undersökning.
Arbetet resulterade i intervjusvar från 11 företag. Det visade sig att företagens medvetenhet om deras licenshantering varierade stort, vilket även att storleken på företagen och innehav av en policy för licenshantering bland företagen gjorde. Metoden för licenshantering och licensinventering skiljde sig åt bland företagen.
Ett flertal program, tillammans med pärmar och Excel-listor var de dominerande licenshanteringsmetoderna bland företagen. Mjukvarutillverkarna och deras intresseorganisationer däribland BSA ställer krav på att företag som använder deras licensierade programvaror skall ha en strukturerad licenshantering. De ser gärna att företagen följer någon vedertagen metod för detta, en sådan metod är Software Asset Management, SAM.
Det är svårt att rekommendera någon licenshanteringsmetod och program som fungerar för samtliga företag, då användandet av dessa är väldigt företags- och organisationsspecifikt. De flesta större mjukvaror som finns i dagsläget följer en vedertagen metod för licenshantering och fungerar därmed bra att tillförlitligt inventera licenser med.
In a community where copyright and intellectual property law is getting more attention in the media, it is important for companies to be aware of their possession of software licenses. In this thesis we are going to investigate the awareness of the software licensing situation in some companies. To collect data that can be analyzed, we are interviewing companies. The interviews are performed by phone or e-mail. A similar report hasn’t been done before therefore it’s an interesting subject to look at.
The interviews resulted in answers from 11 companies. The answers showed that the awareness about software licensing among the companies had big variations. Some other things that varied among the companies were the size of the companies and the usage of software licensing policy within the companies. The way and method of handling software licensing also varied among the companies.
A lot of software programs, along with folders and Excel-lists dominated the method of handling software licenses among the companies. Software developing companies together with their interest organization for example BSA has demands on companies using their software to have a foreseeable documentation for the software licenses. They gladly see that the companies uses a best practice method to manage their license possessions, one best practice method is Software Asset Management, SAM.
It is hard to recommend a method for software license management to a non-specific company, because every company has a unique organization and computer network structure. Most of the big license management software is following a best practice method for software license management.
Bonomo, Renata Cristina Ferreira. "Termodinâmica, modelagem e simulação do processo de adsorção e dessorção de BSA e β-lactoglobulina em cromatografia de interação hidrofóbica". Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/9143.
Повний текст джерелаMade available in DSpace on 2016-11-10T17:39:17Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1573232 bytes, checksum: 0e891caf5f28f0d44ad97897309027c3 (MD5) Previous issue date: 2005-03-01
Neste trabalho foi estudado o processo de adsorção das proteínas albumina de soro bovino (BSA) e β-lactoglobulina (β-lg), provenientes do soro de queijo, em cromatografia de interação hidrofóbica variando-se as condições de temperatura e concentração de sal. Com os dados experimentais obtidos foi conduzida uma análise termodinâmica do processo. Foram realizadas, ainda, modificações no software SimuCromWin, desenvolvido por Saraiva (2003), que consistiram na inclusão da opção de utilização de outros modelos de isotermas além do modelo de Langmuir e o desenvolvimento de um programa que simula o processo de eluição em leito fixo. Avaliou-se, então, a influência dos modelos de isotermas na simulação do processo de adsorção assim como a otimização do processo de adsorção e dessorção das referidas proteínas em cromatografia de interação hidrofóbica. A partir dos resultados obtidos por meio do estudo da adsorção de BSA e β-lg em resina hidrofóbica (Streamline Phenil) foi possível observar que este processo é dependente da concentração de sal. Verificou-se, também, que o processo é espontâneo e entropicamente dirigido. Com as alterações no software SimuCRomWin, foram realizados estudos que indicaram o modelo de isoterma de Langmuir o melhor para simulações em cromatografia preparativa. Além disso, foi realizada a otimização do processo de separação das proteínas albumina de soro bovino (BSA) e β-lactoglobulina (β- lg), utilizando o software SimuCromWin e a técnica de superfície de resposta, na qual verificou-se que em temperaturas e concentrações de sal mais altas, dentro da faixa estudada, a resolução entre os picos é maior.
In this work was studied the adsorption process of whey proteins bovin serum albumin (BSA) and β-lactoglobulin (β-lg) at hydrophobic interaction chromatography (HIC) at four temperatures and salt concentrations. The thermodynamic analysis of the adsorptive process was made using the experimental data. Modifications were accomplished in the software SimuCromWin, developed by SARAIVA (2003), to promote the use of six types of isotherms and to include the elution stage of adsorption in fixed beds. Then, the influence of different isotherm models on the adsorption and dessorption process of BSA and β-lg and the optimization of these processes were studied. The results showed that the adsorption process of BSA and β-lg on Streamline Phenyl is dependent on salt and temperature for the two proteins. The adsorption process is driven by entropy and is favorable for both proteins. Studies were made after the modifications on the software SimuCromWin and indicated the Langmuir isotherm was the best isotherm model to simulated adsorption process for both proteins. The obtained experimental data, software SimuCromWin and surface response analysis permitted to determine the best conditions for adsorption and dessorption processes. The values found to adsorption process were 0.9 M and 313.15 K and to dessorption process were 0.85 M and 313.15 K, for both proteins.
Boiangiu, Clara Dana. "Contribution physiologique des canaux mécanosensibles et des protéines de la famille BspA dans la protection contre le stress osmotique chez Bacillus subtilis et Erwinia chrysanthemi." Rennes 1, 2001. http://www.theses.fr/2001REN10087.
Повний текст джерелаYasuda, Isao. "Pulmonary Stenosis with Intact Ventricular Septum: Assessment and Indication of Reconstructive Surgery for Residual Right-Ventricular Outflow Tract Obstruction." Thesis, Georg Thieme, 1991. http://hdl.handle.net/2237/16685.
Повний текст джерела