Дисертації з теми "Brd7"
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Tocco, Francesca. "Modulation of p53 activities by the prolyl-isomerase PIN1 and the bromodomain protein BRD7." Doctoral thesis, Università degli studi di Trieste, 2008. http://hdl.handle.net/10077/2622.
Повний текст джерелаABSTRACT: MODULATION OF p53 ACTIVITIES BY THE PROLYL-ISOMERASE PIN1 AND THE BROMODOMAIN PROTEIN BRD7 The tumour suppressor p53 belongs to a family of transcription factors that play key roles in maintaining genomic stability and cellular homeostasis. The orchestration of the appropriate cellular responses depends on the fine regulation of p53’s functions through post-translational modifications and interaction with other proteins. In many years of intense study a considerable knowledge on p53 activity has been achieved, yet a greater insight is needed on the specificity of its response. In the first part of this thesis a novel mechanism in the regulation of p53-mediated apoptotic response has been disclosed. It has been demonstrated that upon severe stress signalling p53 dissociates from iASPP, an anti-apoptotic co-factor that inhibits p53 apoptotic functions, and that key roles in this process are played by the prolyl isomerase Pin1. Moreover, it emerged that phosphorylation at p53 Ser46 is required for Pin1-mediated dissociation of the p53-iASPP complex thus providing a mechanistic explanation for the relevance of this site in p53 mediated apoptosis. Notably, the role of Pin1 in assisting the dissociation of p53 from iASPP appears to be independent from Pin1-induced acetylation of p53 and dissociation from Mdm2, further confirming that Pin1 may modulate p53 activity at different levels. A different approach to gain insight on the mechanisms governing p53 regulation is the analysis of p53 protein interaction profiles. The bromodomain containing protein Brd7 was identified as a common interactor of the p53 family proteins in a yeast two hybrid screening conducted in our lab. The presence of the bromodomain and evidences from literature made Brd7 a promising candidate for modulating the p53 pathway at the transcriptional level. This protein and its functional interaction with p53 have been therefore characterized in the second part of this thesis. Upon depletion of Brd7 expression in cells it has been demonstrated that Brd7 is required for efficient cell-cycle arrest in U2OS cells upon challenging with genotoxic stimuli. This effect appeared to be due to a reduction in p21 expression that occurred upon Brd7 depletion and under stress condition. The down-regulation of p21 as a consequence of Brd7 silencing occurred at the transcriptional level and proved to be p53-depedent. Taken together the data reported in the second part of this thesis suggest a role for Brd7 as a positive regulator of p53 transcriptional activity during cell-cycle arrest response and that this function might be exerted by regulating p53-mediated transcription on a chromatin context. Further analysis is needed to dissct the role of this functional interaction. Yet, preliminary investigation suggest that Brd7 might be an important modulator of p53 response and that it can be an important means for p53 to crosstalk with other signalling pathway. Together, the data presented in this thesis contribute to achieve greater knowledge on the mechanism that govern p53 response. As the p53 pathway is compromised to some degree in almost all human cancers, this would be also of great relevance in designing new targeted strategies for cancer treatment.
Riassunto: Modulazione delle attività di p53 da parte della prolyl isomerasi Pin1 e della proteina contenente dominio Bromo Brd7 L’oncosoppressore p53 appartiene a una famiglia di fattori di trascrzione che svolge un ruolo fondamentale nel mantenimento della stabilità genomica e dell’omeostasi cellulare. L’attuazione di un’appropriata risposta cellulare dipende molto dalla regolazione fine delle funzioni di p53. Ciò avviene attraverso modificazioni post-traduzionali e interazioni con altre proteine cellulari. In molti anni di intenso studio si è raggiunta una notevole conoscenza sull’ attività di p53 ma ancora non è stato definito cosa regoli la specificità della risposta. Nella prima parte di questa tesi è stato portato alla luce un nuovo meccanismo nella regolazione della risposta apoptotica mediata da p53. E’ stato dimostrato che, in seguito a stress intensi, p53 si dissocia da iASPP, un co-fattore anti-apoptotico che inibisce le funzioni apoptotiche di p53 e che la prolyl-isomerasi in1 gioca un ruolo fondamentale in questo processo. In aggiunta è emerso che la fosforilazione di p53 al residuo Ser46 è necessaria al distacco di p53 da iASPP mediato da Pin1, fornendo una spiegazione meccanicistica alla nota rilevanza di questo siro per l’apoptosi mediata da p53. Interessantemente, il ruolo di Pin1 nell’assistere la dissociazione di p53 da iASPP sembra essere indipendente dalla sua capacità di favorirne l’acetilazione e il distacco da Mdm2, così confermando che Pin1 può modulare l’attività di p53 a diversi livelli. Un differente approccio per avere delucidazioni sui meccanismi che governano la regolazione di p53 è l’analisi del profilo di interazione proteica. La proteina Brd7 (contenente dominio Bromo) è stata identificata come comune interattore dei membri della famiglia di p53 in uno screening di doppio ibrido in lievito, condotto nel nostro laboratorio. La presenza del dominio Bromo e alcune evidenze di letteratura hanno reso Brd7 un candidato promettente come modulatore trascrizionale della via di segnalazione di p53. Brd7 e la sua interazione funzionale con p53 sono stati caratterizzati nella seconda parte di questa tesi. Dopo aver bloccato l’espressione di Brd7 in cellule è stato dimostrato che Brd7 è necessario per un efficiente arresto del ciclo cellulare in seguito a danni genotossici. Questo effetto sembra essere dovuto ad una riduzione nell’espressione di p21 che avviene I seguito alla deplezione di Brd7 e in condizioni di stress.Tale riduzione avviene a livello trascrizionale ed è p53-dipendente. Insieme I dati riportati nella seconda parte della tesi indicano un ruolo per Brd7 come regolatore positivo dell’attività trascrizionale di p53 durante l’arresto del ciclo cellulare e ciò può avvenire attraverso una regolazione a livello della cromatina. Insieme, I dati presentati in questa tesi contribuiscono ad una maggiore conoscenza sui meccanismi che governano la risposta di p53. Dato che la via di segnalazione di p53 è compromessa a qaulche livello in quasi tutti i tumori, questo potrebbe essere rilevante per disegnare nuove terapie mirate per la cura del cancro.
1978
Comel, Anna. "p53 at the crossroads between cancer and neurodegeneration: unveiling molecular circuitries involved in tumorigenesis and neuronal cell death." Doctoral thesis, Università degli studi di Trieste, 2014. http://hdl.handle.net/10077/10114.
Повний текст джерелаCancer and neurodegeneration are linked by a relation of inverse comorbidity, cancer patients being at lower risk for neurodegenerative disorders and vice versa. Interestingly, many cellular processes and factors contribute to both pathologies, and a central role is played by the transcription factor p53. Best known for its antiproliferative activities following transformation-related stimuli, p53 acts to maintain genetic stability and prevent tumour onset by transcriptional and non-transcriptional mechanisms. Recently, a contribution of p53 also in neuronal development and death was unveiled. In the case of Huntington’s Disease (HD), p53 mediates cytotoxicity in HD cells and animal models, whereas its inhibition prevents this phenotype. On these premises, we were prompted to investigate the signalling pathways and protein interactions that modulate p53 activation in both cancer and neurodegeneration with the aim of identifying critical hubs as new targets for therapeutic intervention. We discovered that expression of HD causative agent, i.e. mutant Huntingtin (mHtt) protein, behaves like a genotoxic stimulus in inducing phosphorylation of p53 on Ser46, that leads to its modification by phosphorylation-dependent prolyl-isomerase Pin1 and consequent induction of apoptotic target genes. Inhibition of Ser46 phosphorylation by targeting HIPK2, PKCδ, or ATM kinases, as well as inhibition of Pin1, prevented mHtt-dependent apoptosis of neuronal cells. These results provide a rationale for the use of inhibitors of stress-responsive kinases and Pin1 as a potential therapeutic strategy for HD treatment. On the other hand, we investigated the contribution of BRD7, a protein involved in epigenetic regulation, to the p53 pathway. We found that BRD7 is required for the onset of oncogene-induced senescence, a main tumour suppressive p53 activity. In addition, we found that upon oncogene activation BRD7 restrains, independently of p53, the acquisition of malignant phenotypes, such as migration/invasion and stem cell traits. We observed a strong induction of inflammatory genes after depletion of BRD7, whose contribution to this process is still under investigation. BRD7 takes part into SWI/SNF and PRC2 chromatin remodelling complexes, whose pleiotropic roles in tumorigenesis make them appealing targets for cancer therapy. We will discuss how this new generated knowledge could be exploited for the treatment of neurodegenerative diseases, in which chromatin alterations are now recognized as drivers of pathogenesis.
XXV Ciclo
1983
De, Deken Joachim. "A BRDF analysis of cloth." Diss., [La Jolla] : University of California, San Diego, 2010. http://wwwlib.umi.com/cr/fullcit?p1477896.
Повний текст джерелаTitle from first page of PDF file (viewed July 14, 2010). Available via ProQuest Digital Dissertations. Includes bibliographical references (leaves 56-60).
Kováčová, Kristýna. "Vyhlídková věž v oblasti Brdy." Master's thesis, Vysoké učení technické v Brně. Fakulta stavební, 2019. http://www.nusl.cz/ntk/nusl-392127.
Повний текст джерелаTongbuasirilai, Tanaboon. "Accurate BRDF Modelling for Wide Angle Scattering." Thesis, Linköpings universitet, Medie- och Informationsteknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-102746.
Повний текст джерелаMatěnová, Lucie. "Vývoj místní samosprávy v Mníšku pod Brdy." Master's thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-257346.
Повний текст джерелаRahman, Shaila. "Molecular Insight into Function of the Evolutionarily Conserved Brd4 Extraterminal Domain (ET) and Mechanism of Brd4 Functions in Human Diseases." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10204.
Повний текст джерелаŘehořková, Jarmila. "Návrh řízení a propagace turistické destinace Brdy-Vltava." Master's thesis, Vysoká škola ekonomická v Praze, 2010. http://www.nusl.cz/ntk/nusl-75182.
Повний текст джерелаBoyer, Jacob, Janos C. Keresztes, Wouter Saeys, and John Koshel. "An automated imaging BRDF polarimeter for fruit quality inspection." SPIE-INT SOC OPTICAL ENGINEERING, 2016. http://hdl.handle.net/10150/622517.
Повний текст джерелаPetzová, Terezie. "Možnosti rozvoje udržitelného cestovního ruchu na území CHKO Brdy." Master's thesis, Vysoká škola ekonomická v Praze, 2017. http://www.nusl.cz/ntk/nusl-359466.
Повний текст джерелаDONATO, ELISA. "MECHANISTIC INVESTIGATION OF BRD4 INHIBITION IN MYC DEPENDENT TUMORS." Doctoral thesis, Università degli Studi di Milano, 2016. http://hdl.handle.net/2434/366358.
Повний текст джерелаGustavsson, Veronica. "Lymfocytstimulering med två olika metoder BrdU-ELISA och CFSE-infärgning." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-58601.
Повний текст джерелаDeniel, Jean-Marc. "Modélisation des luminaires et des BRDF : réalisation, mesure et compression." Rennes 1, 2002. http://www.theses.fr/2002REN10015.
Повний текст джерелаKožíšková, Lucie. "Analýza plnění rozpočtu v konkrétních podmínkách města Mníšek pod Brdy." Master's thesis, Vysoká škola ekonomická v Praze, 2008. http://www.nusl.cz/ntk/nusl-4867.
Повний текст джерелаLatif, Anne-Louise. "Dual inhibition of BRD4 and MDM2 cooperate to eradicate AML." Thesis, University of Glasgow, 2017. http://theses.gla.ac.uk/8189/.
Повний текст джерелаLazardzig, Jan. "Homo ludens, BRD.: Zu Werner Ruhnaus Spielraumkonzeptionen." Hochschule für Musik und Theater 'Felix Mendelssohn Bartholdy' Leipzig, 2019. https://slub.qucosa.de/id/qucosa%3A75495.
Повний текст джерелаOuarets, Shiraz. "Conception de la détection d'un gonioreflectomètre métrologique pour mesurer la BRDF des surfaces." Thesis, Paris, CNAM, 2015. http://www.theses.fr/2015CNAM0995/document.
Повний текст джерелаThe aim of this thesis is to provide the French Metrology with a reference instrument that can be used for measuring the appearance of objects. Several physical properties of object appearance are used not only to identify the object itself but also determine its quality. These include color, gloss, texture and translucency. This work describes the design, characterization and the implementation of an instrument that has been built particularly to measure the bidirectional reflectance distribution function (BRDF) of planar surfaces. BRDF is a physical quantity that enables measuring the surface color and gloss. Accordingly it is important to ensure accurate and traceable measurements of such a quantity in order for a comprehensive understanding of surface properties to be fulfilled.Some surfaces show pigmented, metallic, sparkling or pearlescent appearance. These surfaces, called goniochromatic surfaces, have the ability to change their appearance depending on the viewing angle. To characterize them, it is necessary to deliver surface reflectance measurements at several directions and under different illumination conditions. The instrument that enables these measurements is called gonioreflectometer.The experimental setup we have designed consists of two separate detection lines. The first line, called "color line", is used to study the color. It consists of a stationary illumination source that uses a xenon lamp spectrally resolved by a monochromator. The light coming out from the source is focused on the surface under investigation. The reflected light by the surface is collected by using a lens and directed onto a photodiode by a mirror. This detection system can be moved over a wide angular range so as to measure the BRDF.The second line, called "gloss line", is used to measure the gloss of the surface. In order to be very close to the average human observer, high angular resolution is required. In this line the detection part is stationary. It consists of a Charged Coupled Device (CCD) camera of 512 x 512 pixels. The source used to illuminate the surface is an incandescent tungsten lamp. Angular measurements in this line are provided by the movement of the illumination source itself. Both color and gloss lines share a robot sample-holder that provides six degrees of freedom. This of course facilitates measurements over varies detection and illumination directions of the sample.Key words: Color, Gloss, BRDF, Goniochromatisme, Specular pic
Grísa, Tomáš. "Normalizace hyperspektrálních obrazových dat." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2013. http://www.nusl.cz/ntk/nusl-230517.
Повний текст джерелаMorinière, Jeanne. "La compaction de la chromatine au cours de la spermatogenèse : rôle des bromodomaines de la protéine Brdt." Grenoble 1, 2008. http://www.theses.fr/2008GRE10181.
Повний текст джерелаWang, Xin [Verfasser]. "The Role of BRDT in Esophageal Squamous Cell Carcinoma / Xin Wang." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2021. http://nbn-resolving.de/urn:nbn:de:gbv:7-21.11130/00-1735-0000-0005-153B-2-6.
Повний текст джерелаNunes, Augusto Luengo Pereira. "Rendering baseado em amostragem de BRDF de órgaos vivos por videolaparoscopia." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/105049.
Повний текст джерелаMinimally invasive surgeries are an important specialty of Medicine. Virtual simulators allow the development of the needed skills for new surgeons. Such simulators demand high visual quality of the internal organs that ideally can be performed based on higher-order approximations of the light-material interaction. Recent work proposes hybrid approaches where the BRDF (Bidirectional Reflectance Distribution Function) data for living organs was sampling or estimated to guide real-time rendering techniques. This work proposes a pipeline for physically-based rendering of organic structures with the goal of surgery simulations with a high level of approximation for the light-material interaction. We present a new sampling method for measuring BRDFs for living organs based on conventional laparoscopy. With this data we are able to render human organs in real-time and also improve global illumination results. The methodology was applied in an experiment performed through a Cholecystectomy. Our results achieved a high BRDF coverage of 22% for a living human liver, establishing a singular contribution for the sampling of BRDF in-vivo organs and physically-based rendering.
Yajjou, Halima. "Études moléculaires et structurales - d’un nouveau mode de dimérisation des intégrases rétrovirales pour développer des modulateurs d’oligomérisation ET - de l’intégrase porcine de PERV-A/C en complexe avec son cofacteur cellulaire humain Brd2 dans un contexte de xénotransplantatio." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1035/document.
Повний текст джерелаIntegrase (IN) is an essential enzyme in retroviral replicative cycle, which catalyzes the integration of the viral DNA into the target cell genome. Structural data previously obtained by our team led to rational design of molecules likely to block IN in an inactive oligomeric form. In vitro concerted integration assays made it possible to study their effect on IN enzymatic activity. Pig has an endogenous gammaretrovirus called Porcine Endogenous RetroVirus (PERV) which can be transmitted to humans during xenotransplantation. The aim of the structural study of the recombinant virus PERV-A/C IN is to better understand its mechanism and thus guide the rational design of inhibitors limiting xenozoonosis risk. I modeled the PERV-A/C IN intasome in complex with raltegravir, a drug used for HIV treatment. Then I developed purification protocols to study the isolated and complexed PERV-A/C IN Carboxy-Terminal Domain (CTD) with the human cellular cofactor Brd2. The SAXS envelope of the complex was determined. In parallel, a histone array study, performed with the PERV-A/C IN CTD alone, revealed a specificity profile for modified H2B and H3 histone tails
Gaucher, Jonathan. "Rôle de la protéine à double bromodomaine BRDT dans le remodelage de la chromatine au cours de la spermatogenèse." Thesis, Grenoble, 2011. http://www.theses.fr/2011GRENV088/document.
Повний текст джерелаInvolvement of BRDT in chromatin reorganization during spermatogenesisDuring spermiogenesis, the haploid phase of male gametogenesis, the male genome undergoes a major chromatin reorganization, during which most histones are removed and replaced by transition proteins (TP) and protamines. This process led to the extreme compaction of the genome in the male sperm nucleus.In elongating spermatids, histones are hyperacetylated just before their eviction. We have hypothesized that acetylation of histones mass could be a signal for the removal of histones and recruitment of chromatin remodeling machinery. BRDT is a testis-specific protein, xhich belongs to the BET family, which has two bromodomains able to recognize acetylated histones and has the unique ability to compact hyperacetylated chromatin (Pivot-Pajot et al., 2003). The first of bromodomain BRDT appears crucial for these functions (Morinière et al., 2009). Mice carrying a deletion of the first bromodomaine BRDT, BD1, exhibit male sterility associated with abnormalities occurring during spermiogenesis (Shang et al, 2007). We were able to characterize the physiological function of the first bromodomaine BRDT and demonstrate its crucial role in the replacement of hyperacetylated histones by TP and protamines during spermiogenesis.To explore the potential functions of other domains of the BRDT protein, we have studied mice with invalidation of the Brdt gene. This loss of BRDT also produces male sterility, but the phenotype shows a complete lack of post-meiotic cells. A third mouse model was obtained following our attempt to produce mice with a version of taggued protein. The exploration of these models has demonstrated a role of BRDT, independent of the presence of BD1, in regulating the program of gene expression during entry into meiosis.BRDT has both functions in meiotic and post-meiotic meiotic with the involvement of different protein domains
Labarre, Sébastien. "Caractérisation et modélisation de la rugosité multi-échelle des surfaces naturelles par télédétection dans le domaine solaire." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCC231/document.
Повний текст джерелаSurface roughness is a key property of soils that controls many surface processes and influences the scattering function, or BRDF (Bidirectional Reflectance Distribution Function), of incident radiation. While it is strongly scale-dependent, it is often considered as a stationnary parameter in photometric models. In particular, it is included in the Hapke model as a mean slope angle, integrated over all scales from the grain size to the local topography. Yet its physical meaning is still a question at issue, as the scale at which it occurs is undefined. This thesis aims at understanding how this mean parameter can describe a multiscale phenomenon and to investigate the role of spatial scale on surface BRDF. Finally, we investigate the ability of the Hapke model to retrieve a roughness parameter which is consistent with the ground truth. The topography of volcanic and sedimentary terrains in the Piton de la Fournaise (Réunion Island) and the Asal-Ghoubbet rift (Republic of Djibouti) has been measured using high resolution photogrammetry at a wide range of resolutions thanks to multi-instrumental data : satellite, drone and handheld images. Simultaneously, the BRDF has been numerically simulated, and measured by satellite and a spectrogoniometer (named Chamelon). A multiscale analysis by the means of the wavelet transform reveals the multi-modal behavior of roughness and shows that sub-centimeter surface features dominate both the integrated parameter and the shape of the BRDF. The roughness estimated by inversion of a simplified version of the Hapke model matches the roughness determined over surfaces when the assumptions of the model are verified, with a priori knowledge on surface albedo. The match is not systematic, but remains good for moderately rough terrains using the full Hapke model
Decker, Tim-Michael [Verfasser], and Dirk [Akademischer Betreuer] Eick. "Cdk9 and Brd4 regulate eukaryotic transcription / Tim-Michael Decker ; Betreuer: Dirk Eick." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2017. http://d-nb.info/1149509899/34.
Повний текст джерелаBASUROY, TUPA. "The Role of Bromodomain Containing Protein Nine (BRD9) in Melanogenesis and Melanoma." University of Toledo Health Science Campus / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=mco1535031325513441.
Повний текст джерелаD'Alessandro, Matthew. "Validation study to analyze the suitability of a modified BRDU injection paradigm." Tallahassee, Fla. : Florida State University, 2008. http://purl.fcla.edu/fsu/lib/digcoll/undergraduate/honors-theses/341769.
Повний текст джерелаWoodmansey, James Nicolas. "Structural Insights into Human Epigenetic Regulation from the Proteins SETD7 and Brd3." Thesis, The University of Sydney, 2021. https://hdl.handle.net/2123/24610.
Повний текст джерелаCamargo, Marília de Paiva. "Caracterização das espermatogônias indiferenciadas e dos nichos espermatogoniais em Astyanax altiparanae Garutti e Britski, 2000 (Teleostei, Characidae)." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-12082016-094920/.
Повний текст джерелаObserving by light and transmission electron microscope, undifferentiated spermatogonia of lambari (Astyanax altiparanae), species of great economic and commercial value, they are divided into Aund * and Aund. The type Aund* presents irregular nucleus, eccentric and decondensed chromatin, and nuages near the nuclear envelope and/or associated to mitochondria. The type Aund presents spherical central nucleus, with slightly condensed chromatin and nuages. Morphometric analysis showed that the niche of the type Aund* spermatogonia is near the interstitium, while the type Aund, next to the regions between two tubules (intertubule). Additionally, by the BrdU incorporation and retention technique, we found the possible candidates to spermatogonial stem cells, called \"labeling-retaining cells\", since these cells have retained BrdU over the entire study period (21 days), showing a long cell cycle, a stem cells characteristic.
Castro, Mariana Moraes de. "Duração do ciclo do epitélio seminífero e produção espermática de Leopardus pardalis, Linnaeus, 1758." Universidade Federal de Viçosa, 2012. http://locus.ufv.br/handle/123456789/5093.
Повний текст джерелаO estudo da morfologia testicular e do processo espermatogênico em animais silvestres são fundamentais para o conhecimento dos padrões fisiológicos, auxiliando assim no estabelecimento de protocolos em reprodução assistida. Os objetivos deste estudo foram descrever a duração do ciclo do epitélio seminífero pela identificação das células germinativas mais avançadas marcadas pela 5-bromodeoxiuridina (BrdU); caracterizar os diversos estádios que constituem o ciclo do epitélio seminífero com base na associação das técnicas de morfologia tubular e desenvolvimento do sistema acrossômico; calcular a produção espermática diária por grama de testículo de jaguatirica (Leopardus pardalis). Para isso, foram utilizados cinco animais machos adultos provenientes do Centro de Triagem de Animais Silvestres da Universidade Federal de Viçosa e da Fundação Zoobotânica de Belo Horizonte, ambas em Minas Gerais, Brasil. Os animais foram submetidos a biópsias testiculares a fim de se obter material biológico para avaliação histológica e imunohistoquímica. Foram descritos e determinada a frequência relativa de oito estádios do ciclo do epitélio seminífero em jaguatirica com base no método da morfologia tubular associado ao método do sistema acrossômico. Foi observado um estádio do ciclo por secção transversal de túbulo sendo a fase pré meiótica do processo espermatogênico maior que as outras (56,5%). Através do uso de um marcador de proliferação celular injetado intratesticularmente (BrdU) foi observado após 16 dias de aplicação, células marcadas em transição de paquíteno para diplóteno no início do estádio III. A partir daí, pode-se concluir que um ciclo do epitélio seminífero de jaguatirica dura 11,30 dias, assim o processo espermatogênico da jaguatirica dura no total 50,85 dias. A jaguatirica apresentou diâmetro médio de túbulos seminíferos de 211,37μm e 17,81 metros por grama de testículo em média. No epitélio seminífero, em cada secção transversal do túbulo seminífero no estádio I do ciclo, foi observado em média 1,06 espermatogônias do tipo A; 17,78 espermatócitos primários em pré-leptóteno/leptóteno; 19,22 espermatócitos primários em paquíteno; 59,5 espermátides arredondadas e 6,86 células de Sertoli. O rendimento geral da espermatogênese nesta espécie foi de aproximadamente 57 células, e cada célula de viiiSertoli foi capaz de sustentar e manter 14,38 células da linhagem germinativa das quais, 8,76 espermátides arredondadas. A produção espermática diária encontrada demonstra que a jaguatirica é um animal com alto nível de produção, uma vez que sua produção é cerca de 32 milhões de espermatozóides por grama de testículo.
The study of testicular morphology and spermatogenic process in wild animals are fundamental to the knowledge of physiological patterns, thus assisting in establishing protocols for assisted reproduction. The objectives of this paper were to describe the cycle of the seminiferous epithelium by identifying the most advanced germ cells marked by 5-bromodeoxyuridine (BrdU); to characterize the various stages that constitute the cycle of the seminiferous epithelium based on the combination of the techniques of tubular morphology and development of the acrosomal system, calculate the daily sperm production per gram of testis of ocelot (Leopardus pardalis). For this, we used five adult males from Centro de Triagem de Animais Silvestres at Universidade Federal de Viçosa and from Fundação Zoobotânica de Belo Horizonte, both in Minas Gerais, Brazil. The animals underwent testicular biopsies in order to obtain biological material for histology and immunohistochemistry. We described and determined the relative frequency of eight stages of the seminiferous epithelium cycle on the ocelot based in the tubular morphology method associated with the acrosomal system method. We observed one stage of the cycle by tubule cross-section, being that the pre-meiotic spermatogenic process is longer than the others (56.5%). Through the use of a cell proliferation marker intratesticularly injected (BrdU) we observed after 16 days of application, labeled cells in transition from pachytene to diplotene at the beginning of stage III. From there, we can conclude that a cycle of the seminiferous epithelium of ocelot takes 11.30 days, so the ocelot spermatogenesis process lasts 50.85 days. The ocelot had an average diameter of seminiferous tubules of 211.37 μm and 17.81 meters per gram of testis on average. In the seminiferous epithelium, in each cross-section of seminiferous tubule in stage I of the cycle, we observed on average 1.06 type A spermatogonia, 17.78 primary spermatocytes in pre-leptotene/leptotene, 19.22 primary spermatocytes in pachytene, 59.5 round spermatids and 6.86 Sertoli cells. The overall yield of spermatogenesis in this species was approximately 57 cells, and each Sertoli cell was able to sustain and maintain 14,38 germline cells of which 8.76 were round xspermatids. The daily sperm production found shows that the ocelot is an animal with a high level of production, since production is about than 32 million sperm per gram of testis.
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Chahpazoff, Margaux. "Étude moléculaire et structurale de l’intégrase du virus porcin PERV et de son partenaire cellulaire humain Brd2, dans le cadre de la prévention de xénozoonoses rétrovirales." Thesis, Lyon, 2020. http://www.theses.fr/2020LYSE1003.
Повний текст джерелаXenotransplantation is the transplantation of organs, tissues or cells from an animal to a human recipient and appears to be a promising option regarding the lack of human donors. Research is now focusing on pig donors, but this animal carries an active endogenous Gammaretrovirus, termed PERV (Porcin Endogenous RetroVirus), able to infect patients. Recombinant PERV-A/C IN is a key enzyme of the retroviral life cycle and a relevant therapeutic target. IN requires a cellular cofactor, the human protein Brd2, which allows to target specific integration sites and is mandatory for a productive infection. During this thesis, residues essential for the IN:Brd2 interaction were identified and a SAXS envelope of a two fragments complex were determined. With the support of these experimental results, a first model of the gammaretroviral intasome with a bound Brd2 fragment was built. Finally, a purification protocol of this macromolecular assembly was set-up and electron microscopy images were collected. A first screening of putative IN:Brd2 interaction modulators was carried out with the purpose of developing a therapy limiting xenozoonosis risks
Feng, Xiaofan. "Comparison of methods for generation of absolute reflectance factor measurement for BRDF studies /." Online version of thesis, 1990. http://hdl.handle.net/1850/10922.
Повний текст джерелаRoberts, Gareth James. "Monitoring land cover dynamics using linear kernel-driven BRDF model parameter temporal trajectories." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.407145.
Повний текст джерелаMendes, Jose Manuel Correia. "Seleção e encaminhamento de projetos de investimentos : caso SEBRAE-RS, BRDE e CRP." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/2112.
Повний текст джерелаKhan, Younis. "The role of NF-κB p65 and Brd4 in oxidative stress driven inflammation". Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/14702.
Повний текст джерелаVisintin, Francesca <1971>. "La valutazione economica del paesaggio rurale in una zona vitivinicola della Slovenia (BRDA)." Doctoral thesis, Università Ca' Foscari Venezia, 2004. http://hdl.handle.net/10579/633.
Повний текст джерелаSantos, Nilton Pedro dos. "Regeneração hepática em bagre africano (Clarias gariepinus) após hepatectomia parcial." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/74/74131/tde-23042004-135041/.
Повний текст джерелаThe liver is an important organ in studies aimed to the verification of animals reactions to chemical injuries. The partial hepatectomy (PH) is one of the methods that are usually employed in hepatic regeneration experiments. Our objectives in this work were to verify the influence of 30% and 70% PH on the hepatic cells proliferation index as it is measured by BrdU nuclear uptake in two regions: close to the hepatic surgery region and in a farther region. The biliary ducts and bile preductular epithelial cells (BPDECs) proliferation was quantified with a combination of two antibodies against the human cytokeratins AE1/AE3. The proliferation index peak in 30% hepatectomy animals occurred one day after the surgery, but in 70% hepatectomy animals the peak was only observed after three days from the surgery. Differences between the proliferation index of the close and the far region were not observed and likewise histological differences were absent. In the regions far from the PH site more biliary ducts were counted than in closer regions, but the peaks were coincident in both regions with the hepatocytes proliferation index. It was also observed that the quantity of BPDECs increased. The peaks occurred in the first and third days for the 30% and 70% PH respectively, and both peaks were higher in the region far from the PH site. We may conclude that C. gariepinus liver regeneration after 30% and 70% PH occurs by means of compensatory hyperplasia by hepatocytes, biliary ducts and BPDECs proliferation. The 30% HP stimulated a strong regenerative response and is less traumatic to the animals. The regions far from the PH site reacts more intensely that the close region concerning the stimulation of biliary ducts and BPDECs proliferation.
Lenot, Xavier. "Modélisation et inversion d'images hyperspectrales : quantification et prise en compte du relief et de la réflectance bidirectionnelle." Toulouse, ENSAE, 2005. http://www.theses.fr/2005ESAE0009.
Повний текст джерелаChermain, Xavier. "Rendu basé physique de micro-reflets." Thesis, Limoges, 2019. http://www.theses.fr/2019LIMO0070/document.
Повний текст джерелаGlint rendering, useful for simulating the appearance of glittery materials, brushed metal or scratched plastic, is a theoretical and technical challenge in computer graphics. It involves the use of spatially varying patch bidirectional reflectance distribution functions (P-BRDFs) with high frequencies. In this thesis we propose two new P-BRDFs based on specular normal maps. Unlike the previous method [YHMR16], our first BRDF prevents any creation of energy through footprint-dependent normalisation. This normalisation is possible thanks to a new representation of the normal map based on a mixture of non-centered and non-axis aligned Beckmann NDFs. The second method improves the first one and prevents, for the first time, any creation and loss of energy, by simulating multiple scattering in the microgeometry. It enables artifact-free rendering of opaque and sparkling surfaces. In addition, we provide an optimal sampling algorithm using the visibility information of the normals. The key idea of this method is the definition of a V-cavity for each point of the surface. To simulate multiple scattering inside it, we compensate for the energy lost by a single scattering model, by reintegrating lost energy with an energy compensation BRDF. The rendering time and memory footprint of our methods are in the same order of magnitude than previous methods
Krieger, Björn Harald. "Die Notwendigkeit einer Stärkung der privaten Altersvorsorge in der BRD /." Hamburg : Kovač, 2007. http://www.verlagdrkovac.de/978-3-8300-3051-5.htm.
Повний текст джерелаKrumpal, Ivar, and Heiko Rauhut. "Dominieren Bundes- oder Landesparteien die individuellen Landtagswahlentscheidungen in der BRD?" Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-208326.
Повний текст джерелаElectoral studies often interpret German regional election results ("Landtagswahlen") as barometers of public opinion on federal governmental parties' performance. However, while interpreting German regional elections as "test-elections" for the national parliament, it is often underemphasised that subnational elections often follow a unique regional dynamics. So far, empirical investigations on the determinants of German regional elections consist either in qualitative case studies or aggregate analyses of official statistics. A quantitative study of individual-level survey data, comparing directly the explanatory power of the federal versus the subnational level, is still lacking. Conducting a repeated survey design, we analyse data from 17 German regional election surveys. Thus, the effects of individual assessments of federal parties versus their subnational counterparts on subnational voting preferences can be directly compared. The conclusion of our analyses can be summarized as follows: In Western Germany, the valuation of subnational parties has a stronger impact on individual voting preferences in subnational elections than the valuation of the federal parties has. However, in Eastern Germany, the federal dimension has a comparatively stronger effect. Hence, the federal – regional ("Länder") party system linkage is clearly stronger in Eastern than in Western Germany
Weiss, Stefan. "Penderecki und Ligeti: Zur Rezeption der Klangkomposition in der BRD." Gudrun Schröder Verlag, 2006. https://ul.qucosa.de/id/qucosa%3A70572.
Повний текст джерелаZimmermann, Suzanne. "Sexualpädagogik in der BRD und in der DDR im Vergleich /." Giessen : Psychosozial, 1999. http://catalogue.bnf.fr/ark:/12148/cb372227088.
Повний текст джерелаBuchhorn, Marcel. "Ground-based hyperspectral and spectro-directional reflectance characterization of Arctic tundra vegetation communities : field spectroscopy and field spectro-goniometry of Siberian and Alaskan tundra in preparation of the EnMAP satellite mission." Phd thesis, Universität Potsdam, 2013. http://opus.kobv.de/ubp/volltexte/2014/7018/.
Повний текст джерелаDie arktische Tundra ist mit circa 5,5 % der Landoberfläche eines der letzten großen verbliebenen fast unberührten Ökosysteme unserer Erde. Nur die Fernerkundung ist in der Lage, benötigte Informationen über Struktur und Zustand dieses Ökosystems großräumig und in regelmäßigen Zeitabständen zur Verfügung zu stellen. Aber fast alle natürlichen Oberflächen zeigen individuelle anisotrope Reflexionsverhaltensweisen, welche durch die bidirektionale Reflektanzverteilungsfunktion (englisch: BRDF) beschrieben werden können. Dieser Effekt kann zu erheblichen Veränderungen im gemessenen Reflexionsgrad der Oberfläche in Abhängigkeit von den solaren Beleuchtung- und Blickrichtungsgeometrien führen. Zielstellung dieser Arbeit ist die hyperspektrale und spektro-direktionale Charakterisierung der Oberflächenreflexion wichtiger und repräsentativer arktischer Pflanzengesellschaften in Sibirien und Alaska, als Grundlage für die Extraktion von Vegetationsparametern und die Normalisierung von BRDF-Effekten in Off-Nadir und multi-temporalen Fernerkundungsdaten. In Vorbereitung auf die bevorstehende nationale EnMAP Satellitenmission ist ein Grundverständnis der BRDF-Effekte in der arktischen Tundra von wesentlicher Bedeutung für die Erstellung von hochqualitativen, konsistenten und damit vergleichbaren Datensätzen. Die in dieser Arbeit genutzten Daten beruhen auf geländespektroskopische und geländespektro-goniometrische Untersuchungen von repräsentativen Messflächen in Sibirien und Alaska. Die Entwicklung eines leichten, transportablen und einfach anzuwendenden Geländespektro-Goniometers, welches dennoch zuverlässig Daten liefert, war die erste Aufgabe. Hierfür habe ich ein Gerät mit der Bezeichnung ManTIS („Manual Transportable Instrument platform for ground-based Spectro-directional observations“) entwickelt. Die Ergebnisse der geländespektro-radiometrischen Messungen entlang wichtiger ökologischer Gradienten (regionales Klima, pH-Wert des Bodens, Bodenfeuchte, Toposequenz) zeigen, dass die Pflanzengesellschaften sich anhand ihrer Nadir-Reflektanzen unterscheiden lassen. Insbesondere die Möglichkeit der Differenzierung im sichtbaren (VIS) blauen und roten Wellenlängenbereich. Die Nah-Infrarot (NIR) Schulter und das NIR-Reflektanzplateau sind trotz ihrer niedrigeren Reflektanzwerte eine wertvolle Informationsquelle, die genutzt werden kann um die Pflanzengesellschaften entsprechend ihrer Biomasse und der Vegetationsstruktur voneinander zu unterscheiden. Im Allgemeinen zeigen die verschiedenen Pflanzengesellschaften der Tundra: (i) eine niedrige maximale NIR-Reflektanz; (ii) ein schwaches oder nicht sichtbares lokales Reflektanzmaximum im grünen VIS-Spektrum; (iii) einen schmalen „red-edge“ Bereich zwischen dem roten und NIR-Wellenlängenbereich und (iv) kein deutliches NIR-Reflektanzplateau. Diese gemeinsamen Nadir-Reflektanzeigenschaften sind entscheidend für das Verständnis der Variabilität der BRDF-Effekte in der arktischen Tundra. Keine der untersuchten Pflanzengesellschaften wies isotrope Reflektanzeigenschaften auf. Im Allgemeinen zeigt Tundravegetation: (i) die höchsten BRDF-Effekte in der solaren Hauptebene; (ii) die maximalen Reflexionsgrade in den rückwärts gerichteten Blickrichtungen; (iii) höhere Grade an Anisotropie im VIS-Spektrum als im NIR-Spektrum und (iv) schüsselförmige Reflexionsgradverteilungen in den längeren Wellenlängenbereichen (>700 nm). Die Analyse des Einflusses von hohen Sonnenzenitwinkeln auf die Anisotropie der Rückstrahlung zeigt, dass sich mit zunehmenden Sonnenzenitwinkeln die Anisotropie-Eigenschaften in azimutal-symmetrische schüsselförmige Reflexionsgradverteilungen ändern. Auch ergeben die spektro-direktionalen Analysen, dass Fernerkundungsprodukte wie der NDVI oder die relative Absorptionstiefe stark von BRDF-Effekten beeinflusst werden. Die anisotropen Eigenschaften der Fernerkundungsprodukte können sich erheblich von den beobachteten BRDF-Effekten in den ursprünglichen Reflektanzdaten unterscheiden. Auch lässt sich aus den Ergebnissen ableiten, dass der NDVI relativ gesehen die blickrichtungsabhängigen BRDF-Effekte minimieren kann. Für die untersuchten Pflanzengesellschaften der Tundra weichen die Off-Nadir NDVI-Werte nie mehr als 10 % von den Nadir-NDVI-Werten ab. Im Resümee dieser Studie wird nachgewiesen, dass Änderungen in der Sonnen-Objekt-Sensor-Geometrie direkt zu Reflektanzveränderungen in den Fernerkundungsdaten von arktischen Pflanzengesellschaften der Tundra entsprechend ihrer objekt-spezifischen BRDF-Charakteristiken führen. Da die verschiedenen Arten der Tundravegetation nur kleine, aber signifikante Unterschiede in der Oberflächenreflektanz zeigen, ist es wichtig die spektro-direktionalen Reflexionseigenschaften bei der Entwicklung von Algorithmen für Fernerkundungsprodukte zu berücksichtigen.
Martini, Dorival Terra. "Avaliação histológica e imunohistoquímica da ATM de ratos wistar adultos após meniscectomia unilateral com enxerto de cartilagem auricular autógena." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/42/42131/tde-30052008-112522/.
Повний текст джерелаThe morphological features of the temporomandibular joint (TMJ) of rats were studied after meniscectomy in the left side of the joint. The specimens were submitted or not to an interpositional auricular cartilage graft. The animals formed the following groups: GI (meniscectomy); GII (meniscectomy and graft); GIII (sham). The post-operative periods (10, 30 days) and the side allowed to form the subgroups. The articular surface of the TMJ exhibited organized layers (GIII, GId, GIId groups) where the type I collagen fibers arranged in regular nets prevailed. Morphological changes as erosion of the articular surface with large subchondral cysts in the subjacent bone were verified (GIe, GIIe). The condyle\'s surface of GII was regular and structurally preserved under the graft. The type I collagen fibers constituted the articular and chondroblastic layers in the GIe and GIIe animals where the type III collagen fibers were predominant in the prechondroblastic layer. Wide vascular spaces were evident in the bilaminar zone of the GI and GII animals.
Manoharan, Madhu. "Evaluation of a neural network for formulating a semi-empirical variable kernel BRDF model." Master's thesis, Mississippi State : Mississippi State University, 2005. http://library.msstate.edu/content/templates/?a=72.
Повний текст джерелаMurphy, Tami J. "Phenotypic analysis of transcriptional co-activator, brd2, gene knockdowns in zebrafish (Danio rerio) embryos." Click here for download, 2007. http://proquest.umi.com/pqdweb?did=1288659571&sid=1&Fmt=2&clientId=3260&RQT=309&VName=PQD.
Повний текст джерелаShaw, J. "Use of plant growth simulations to validate BRDF model parameters derived SPOT-VGT data." Thesis, Swansea University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.639015.
Повний текст джерелаNAHM, KIEBONG. "LIGHT SCATTERING BY POLYSTYRENE SPHERES ON A CONDUCTING PLANE (MIE, IMAGE CHARGE, INTERFERENCE, BRDF)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/188071.
Повний текст джерелаBurns, Robert. "Comparison of skeletal morphology of Brd2 heterozygous mice against wild type C57/B6J mice." Thesis, Boston University, 2012. https://hdl.handle.net/2144/12302.
Повний текст джерелаObjectives: The purpose of the study was to characterize age related bone lose in male and female mice lacking one functional copy of the Brd2 gene. These mice will develop age related obesity but will not develop Type II diabetes that is normally associated with obesity. Thus age and sex related changes in the skeletal morphology and structure can be assessed in response to obesity but not be confounded by the development of insulin resistance that is known to effect bone mass. Methods: Wild type and Brd2 heterozygote male and female mice were allowed to age under normal conditions in which they were fed ad libitum. Skeletal morphology and structure was assessed via µCT of the proximal metaphysis for the trabecular architecture and for the mid-diaphyseal region to assess cortical. Results: Genotype was found to play a significant role in affecting trabecular architecture in females for BV/TV, connective density, trabecular number, thickness and separation and in males for degree of anisotropy. An age-bygenotype was found to play a significant role in affecting cortical architecture in males for cortical area, total area, cortical area/total area, and medullary area. Conclusion: Brd2 heterozygote mice have significantly altered structural parameters from that of their wild type counterparts. These differences in how genotype affected the sexes suggests that the Brd2 protein is affected by sex dependent mechanism related to general metabolism and adiposity independent of insulin function.