Готові списки джерел за темами / Bleb Dynamics

Добірка наукової літератури з теми "Bleb Dynamics"

Автор: Grafiati
Опубліковано: 7 вересня 2023

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Статті в журналах з теми "Bleb Dynamics"

1

Woolley, Thomas E., Eamonn A. Gaffney, and Alain Goriely. "Membrane shrinkage and cortex remodelling are predicted to work in harmony to retract blebs." Royal Society Open Science 2, no. 7 (July 2015): 150184. http://dx.doi.org/10.1098/rsos.150184.

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Numerous cell types undergo an oscillatory form of dynamics known as blebbing, whereby pressure-driven spherical protrusions of membrane (known as blebs) expand and contract over the cell's surface. Depending on the cell line, blebs play important roles in many different phenomena including mitosis and locomotion. The expansion phase of cellular blebbing has been mathematically modelled in detail. However, the active processes occurring during the retraction phase are not so well characterized. It is thought that blebs retract because a cortex reforms inside, and adheres to, the bleb membrane. This cortex is retracted into the cell and the attached bleb membrane follows. Using a computational model of a cell's membrane, cortex and interconnecting adhesions, we demonstrate that cortex retraction alone cannot account for bleb retraction and suggest that the mechanism works in tandem with membrane shrinking. Further, an emergent hysteresis loop is observed in the intracellular pressure, which suggests a potential mechanism through which a secondary bleb can be initiated as a primary bleb contracts.
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2

Fackler, Oliver T., and Robert Grosse. "Cell motility through plasma membrane blebbing." Journal of Cell Biology 181, no. 6 (June 9, 2008): 879–84. http://dx.doi.org/10.1083/jcb.200802081.

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Plasma membrane blebs are dynamic cytoskeleton-regulated cell protrusions that have been implicated in apoptosis, cytokinesis, and cell movement. Influencing Rho–guanosine triphosphatase activities and subsequent actomyosin dynamics appears to constitute a core component for bleb formation. In this paper, we discuss recent evidence in support of a central role of nonapoptotic membrane blebbing for cell migration and cancer cell invasion as well as advances in our understanding of the underlying molecular mechanisms. Based on these studies, we propose that in a physiological context, bleb-associated cell motility reflects a cell's response to reduced substratum adhesion. The importance of blebbing as a functional protrusion is underscored by the existence of multiple molecular mechanisms that govern actin-mediated bleb retraction.
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3

Chikina, Aleksandra S., Tatyana M. Svitkina, and Antonina Y. Alexandrova. "Time-resolved ultrastructure of the cortical actin cytoskeleton in dynamic membrane blebs." Journal of Cell Biology 218, no. 2 (December 12, 2018): 445–54. http://dx.doi.org/10.1083/jcb.201806075.

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Membrane blebbing accompanies various cellular processes, including cytokinesis, apoptosis, and cell migration, especially invasive migration of cancer cells. Blebs are extruded by intracellular pressure and are initially cytoskeleton-free, but they subsequently assemble the cytoskeleton, which can drive bleb retraction. Despite increasing appreciation of physiological significance of blebbing, the molecular and, especially, structural mechanisms controlling bleb dynamics are incompletely understood. We induced membrane blebbing in human HT1080 fibrosarcoma cells by inhibiting the Arp2/3 complex. Using correlative platinum replica electron microscopy, we characterize cytoskeletal architecture of the actin cortex in cells during initiation of blebbing and in blebs at different stages of their expansion–retraction cycle. The transition to blebbing in these conditions occurred through an intermediate filopodial stage, whereas bleb initiation was biased toward filopodial bases, where the cytoskeleton exhibited local weaknesses. Different stages of the bleb life cycle (expansion, pausing, and retraction) are characterized by specific features of cytoskeleton organization that provide implications about mechanisms of cytoskeleton assembly and bleb retraction.
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4

Petrov, S. Yu, and D. M. Safonova. "Efficacy and Safety of Late Bleb Needling to Prolong Post-Trabeculectomy Hypotensive Effect." Ophthalmology in Russia 15, no. 4 (January 9, 2019): 416–23. http://dx.doi.org/10.18008/1816-5095-2018-4-416-423.

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Purpose. To assess the efficacy and safety of late bleb needling to prolong the hypotensive effect after glaucoma surgery.Patients and Methods. The study included 130 patients (130 eyes) with IOP decompensation after trabeculectomy. The patients were divided into 3 groups: cystous blebs (50 eyes), incapsulated blebs (50 eyes) and biomicroscopically absent blebs that reveal intraocular fluid collections under the sclera flap during optical coherence tomography (30 eyes). All patients underwent tonometry, visometry and bleb hyperemia assessment.Results. Patients with cystous blebs showed a mean IOP reduction from 25.4 ± 3.2 mm Hg to 12.7 ± 4.5 mm Hg the day after needling. During the follow up period the mean IOP level showed a minimal fluctuation within the bounds of 13–15 mm Hg. Incapsulated blebs demonstrated a reduction from 28.9 ± 6.3 to 15.1 ± 4.3 mm Hg in the course of the first day. The following IOP fluctuations remained within a higher range, than in Group 1: 15.9–18.3 mm Hg. IOP dynamics in the visually absent bleb group had its specificts: the initial procedure led to a transient IOP increase from 24.6 ± 4.7 mm Hg to 27,6 ± 3,5 mm Hg, followed by a reduction within the first day to the level 19.4 ± 3.1 mm Hg, with further IOP fluctuations during the follow up period staying withing the range of 14.6–15.8 mm Hg. Performing bleb needling to restore trabeculectomy hypotensive efficacy had a total surgical success in 100 % patients with cystous blebs, that were formed as a result of conjunctival-scleral lesions. Incapsulated bleb needling had a 74 % total success rate and visually absent blebs with intraocular fluid collections under the flap had a 90 % total success rate within the 6 month follow up period.Conclusion. The study shows high efficacy of late needling of different blebs as an invasive measure of restoring post-trabeculectomy hypotensive effect.
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5

Asante-Asamani, Emmanuel, Daniel Grange, Devarshi Rawal, Zully Santiago, John Loustau, and Derrick Brazill. "A role for myosin II clusters and membrane energy in cortex rupture for Dictyostelium discoideum." PLOS ONE 17, no. 4 (April 25, 2022): e0265380. http://dx.doi.org/10.1371/journal.pone.0265380.

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Анотація:
Blebs, pressure driven protrusions of the cell membrane, facilitate the movement of eukaryotic cells such as the soil amoeba Dictyostelium discoideum, white blood cells and cancer cells. Blebs initiate when the cell membrane separates from the underlying cortex. A local rupture of the cortex, has been suggested as a mechanism by which blebs are initiated. However, much clarity is still needed about how cells inherently regulate rupture of the cortex in locations where blebs are expected to form. In this work, we examine the role of membrane energy and the motor protein myosin II (myosin) in facilitating the cell driven rupture of the cortex. We perform under-agarose chemotaxis experiments, using Dictyostelium discoideum cells, to visualize the dynamics of myosin and calculate changes in membrane energy in the blebbing region. To facilitate a rapid detection of blebs and analysis of the energy and myosin distribution at the cell front, we introduce an autonomous bleb detection algorithm that takes in discrete cell boundaries and returns the coordinate location of blebs with its shape characteristics. We are able to identify by microscopy naturally occurring gaps in the cortex prior to membrane detachment at sites of bleb nucleation. These gaps form at positions calculated to have high membrane energy, and are associated with areas of myosin enrichment. Myosin is also shown to accumulate in the cortex prior to bleb initiation and just before the complete disassembly of the cortex. Together our findings provide direct spatial and temporal evidence to support cortex rupture as an intrinsic bleb initiation mechanism and suggests that myosin clusters are associated with regions of high membrane energy where its contractile activity leads to a rupture of the cortex at points of maximal energy.
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6

Cunningham, C. C. "Actin polymerization and intracellular solvent flow in cell surface blebbing." Journal of Cell Biology 129, no. 6 (June 15, 1995): 1589–99. http://dx.doi.org/10.1083/jcb.129.6.1589.

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The cortical actin gel of eukaryotic cells is postulated to control cell surface activity. One type of protrusion that may offer clues to this regulation are the spherical aneurysms of the surface membrane known as blebs. Blebs occur normally in cells during spreading and alternate with other protrusions, such as ruffles, suggesting similar protrusive machinery is involved. We recently reported that human melanoma cell lines deficient in the actin filament cross-linking protein, ABP-280, show prolonged blebbing, thus allowing close study of blebs and their dynamics. Blebs expand at different rates of volume increase that directly predict the final size achieved by each bleb. These rates decrease as the F-actin concentration of the cells increase over time after plating on a surface, but do so at lower concentrations in ABP-280 expressing cells. Fluorescently labeled actin and phalloidin injections of blebbing cells indicate that a polymerized actin structure is not present initially, but appears later and is responsible for stopping further bleb expansion. Therefore, it is postulated that blebs occur when the fluid-driven expansion of the cell membrane is sufficiently rapid to initially outpace the local rate of actin polymerization. In this model, the rate of intracellular solvent flow driving this expansion decreases as cortical gelation is achieved, whether by factors such as ABP-280, or by concentrated actin polymers alone, thereby leading to decreased size and occurrence of blebs. Since the forces driving bleb extension would always be present in a cell, this process may influence other cell protrusions as well.
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7

Asante-Asamani, Emmanuel, Derrick Brazill, and Wanda Strychalski. "Actin-myosin dynamics during bleb stabilization." Biophysical Journal 121, no. 3 (February 2022): 118a. http://dx.doi.org/10.1016/j.bpj.2021.11.2129.

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Davidson, Andrew J., Clelia Amato, Peter A. Thomason, and Robert H. Insall. "WASP family proteins and formins compete in pseudopod- and bleb-based migration." Journal of Cell Biology 217, no. 2 (November 30, 2017): 701–14. http://dx.doi.org/10.1083/jcb.201705160.

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Анотація:
Actin pseudopods induced by SCAR/WAVE drive normal migration and chemotaxis in eukaryotic cells. Cells can also migrate using blebs, in which the edge is driven forward by hydrostatic pressure instead of actin. In Dictyostelium discoideum, loss of SCAR is compensated by WASP moving to the leading edge to generate morphologically normal pseudopods. Here we use an inducible double knockout to show that cells lacking both SCAR and WASP are unable to grow, make pseudopods or, unexpectedly, migrate using blebs. Remarkably, amounts and dynamics of actin polymerization are normal. Pseudopods are replaced in double SCAR/WASP mutants by aberrant filopods, induced by the formin dDia2. Further disruption of the gene for dDia2 restores cells’ ability to initiate blebs and thus migrate, though pseudopods are still lost. Triple knockout cells still contain near-normal F-actin levels. This work shows that SCAR, WASP, and dDia2 compete for actin. Loss of SCAR and WASP causes excessive dDia2 activity, maintaining F-actin levels but blocking pseudopod and bleb formation and migration.
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Hinojosa, Laura Soto, Manuel Holst, Christian Baarlink, and Robert Grosse. "MRTF transcription and Ezrin-dependent plasma membrane blebbing are required for entotic invasion." Journal of Cell Biology 216, no. 10 (August 3, 2017): 3087–95. http://dx.doi.org/10.1083/jcb.201702010.

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Entosis is a nonapoptotic form of cell death initiated by actomyosin-dependent homotypic cell-in-cell invasion that can be observed in malignant exudates during tumor progression. We previously demonstrated formin-mediated actin dynamics at the rear of the invading cell as well as nonapoptotic plasma membrane (PM) blebbing in this cellular motile process. Although the contractile actin cortex involved in bleb-driven motility is well characterized, a role for transcriptional regulation in this process has not been studied. Here, we explore the impact of the actin-controlled MRTF–SRF (myocardin-related transcription factor–serum response factor) pathway for sustained PM blebbing and entotic invasion. We find that cortical blebbing is tightly coupled to MRTF nuclear shuttling to promote the SRF transcriptional activity required for entosis. Furthermore, PM blebbing triggered SRF-mediated up-regulation of the metastasis-associated ERM protein Ezrin. Notably, Ezrin is sufficient and important to sustain bleb dynamics for cell-in-cell invasion when SRF is suppressed. Our results highlight the critical role of the actin-regulated MRTF transcriptional pathway for bleb-associated invasive motility, such as during entosis.
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10

Goudarzi, Mohammad, Aleix Boquet-Pujadas, Jean-Christophe Olivo-Marin, and Erez Raz. "Fluid dynamics during bleb formation in migrating cells in vivo." PLOS ONE 14, no. 2 (February 26, 2019): e0212699. http://dx.doi.org/10.1371/journal.pone.0212699.

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Дисертації з теми "Bleb Dynamics"

1

Kirchmann, Patrick S. "Ultrafast electron dynamics in low-dimensional materials." München Verl. Dr. Hut, 2009. http://d-nb.info/993731325/04.

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2

Soane, Alexander (Alexander Visotsky). "Investigation of asymmetric plasma blob dynamics." Thesis, Massachusetts Institute of Technology, 2009. http://hdl.handle.net/1721.1/46659.

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Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Physics, 2009.
Includes bibliographical references (p. 71).
The dynamics of asymmetric blobs is investigated in the Versatile Toroidal Facility (VTF) at MIT. Blobs are local regions of enhanced plasma density. Blobs are relevant to several areas of physics research, including fusion experiments. Understanding blob dynamics is important because blobs affect plasma confinement, a key issue for fusion experiments. The blobs in this experiment are created under varying magnetic and neutral gas density conditions. We explore several methods for creating these asymmetric blobs and offer theoretical predictions for some of the behavior. Four arrays of Langmuir probes are placed inside the toroidal chamber to measure plasma density. Data collected is shown to confirm the existence of a plasma blob and is analyzed to extract radial and toroidal information. Radial propagation is compared with a previous experiment that featured symmetric blobs. We find that the presence of a vertical magnetic field is a significant variable for radial speed. Toroidal motion is shown to be very fast relative to the radial propagation. This experiment serves as a useful reference for future research on asymmetric blobs.
by Alexander Soane.
S.B.
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3

Ivemark, Biorn. "Bleu blanc noir : assimilation trajectories, identity dynamics and boundary work of French Antilleans, West Africans, and their children in Paris." Thesis, University of British Columbia, 2017. http://hdl.handle.net/2429/62929.

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This study compares the assimilation trajectories, identity dynamics and boundary work of French Antilleans, West Africans and their descendants in the Paris region. While previous studies have focused on the experiences of French Antilleans and sub-Saharan Africans separately or those of Blacks in France as a whole, this study engages in a more minute comparison of the experiences of West African immigrants and French Antilleans across two generations in mainland France. This comparison primarily aims to determine the role of the divergent civic, cultural and religious backgrounds of these groups alongside their largely shared racial characteristics in how they assimilate to French society across two generations. These variables are of particular interest given the salience of civic and cultural distinctions in France, while racial distinctions are notoriously downplayed. The main theoretical goal of the study is to assess the usefulness of segmented assimilation theory in accounting for the various assimilation outcomes of these groups. Drawing on 55 in-depth interviews complemented with wide-ranging statistical data, I explore the impact of cultural, religious and racial factors on the intergenerational educational and professional trajectories of both populations, analyze how these factors influence their identification patterns and assess how members of these groups seek to negotiate the various symbolic boundaries that they come up against, both in their relations to each other and to the majority population. The results suggest that French Antilleans have more favourable educational and professional outcomes than West Africans. Despite the importance of racial barriers for both groups, the findings also underscore the salience of cultural and religious forces as well as the identification dynamics and boundary work that both groups engage in. While some segmented assimilation mechanisms remain valid in the French case, the study also demonstrates the importance of empirically identifying societally specific assimilation barriers and cultural segments for the theory to retain its usefulness in other national contexts.
Arts, Faculty of
Sociology, Department of
Graduate
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4

Calderon, F. A. "Non-linear instabilities in the edge of tokamak plasmas : characterization of edge localized modes and numerical simulation of blob dynamics using a hybrid model." Thesis, University of Warwick, 2015. http://wrap.warwick.ac.uk/77687/.

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Characterization of edge tokamak plasma instabilities by measuring emergent phenomena within a range of frequencies above the ion cyclotron frequency have been explored in two ways: using the inter-event waiting times of Edge Localized Modes (ELMs) occurrences in measured time series of JET plasmas and by performing 2D/3D simulations of filamentary structures dynamics using a hybrid model plasma description, i.e. kinetic ion particles and massless charge neutralizing electron fluid. The analysis of ELMs time series using characteristic emission lines Da of JET tokamak in otherwise similar plasmas was performed with only a minimal number of drivers such as the gas puffing rate. They have shown a key role in changing the underlying system mode cycle where a threshold value revealed its transition from single harmonic behaviour to a transitioning phase into a total lost of the state and born of a higher frequency resonant mode. Hybrid simulations of blobs/filaments are performed in 2D/3D to observe the kinetic evolution of these plasma structures over several ion gyroperiods. Novel 3D simulations represent the first kinetic simulations of these structures along the parallel direction using a kinetic description. We have investigated the evolution and the internal density currents which provide insight of the effects of finite Larmor radius in the blobs dynamics and evolution.
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5

Günther, Susanne [Verfasser], Susann Akademischer Betreuer] Müller, Isolde [Akademischer Betreuer] Röske, and Thomas [Akademischer Betreuer] [Bley. "Population structure and dynamics of polyphosphate accumulating organisms in a communal wastewater treatment plant / Susanne Günther. Gutachter: Isolde Röske ; Thomas Bley. Betreuer: Susann Müller." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://d-nb.info/106773225X/34.

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6

Duval, Perrine. "Dynamique des communautés microbiennes et évolution métabolomique de fromages à pâte persillée, Bleu d'Auvergne et Fourme d'Ambert, sous emballage et atmosphère gazeuse." Thesis, Clermont-Ferrand 2, 2016. http://www.theses.fr/2016CLF22678.

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Анотація:
Cette thèse avait pour objectif de caractériser les interactions entre atmosphère, communautés microbiennes et matrice et apporter des éléments pour la conception d’emballages adaptés à la conservation de fromages à pâte persillée Bleu d’Auvergne et Fourme d’Ambert. Dans cette optique, l’effet d’emballages commerciaux et de différentes atmosphères gazeuses sur d’une part l’activité respiratoire de l’écosystème fromage, d’autre part sur l’évolution biochimique, microbiologique et sensorielle de fromages à pâte persillée AOP d’Auvergne a été évalué. Les portions de fromages emballés sous vide avec des films de différentes perméabilités à l’O2, CO2 , à la vapeur d’eau se distinguaient de ceux emballés en pain entier sous aluminium par des niveaux plus faibles de bactéries d’affinage (Staphylococcus, Arthrobacter, Brevibacterium), par des pH plus bas en lien avec une plus faible production d’ammoniaque. Ils étaient aussi caractérisés par de plus fortes teneurs en méthyl cétones dues à leur moindre dégradation, en alcools secondaires et en esters et des teneurs plus faibles en acides gras et en composés issus de la dégradation des acides aminés ramifiés. Ils étaient perçus comme plus piquants, crémeux avec une odeur de lait, visuellement plus humides mais moins amers que les fromages emballés sous aluminium. Des incubations de fromages bleus d’auvergne en conditions d’O2 et CO2 contrôlées ont montré que l’oxygène jouait un rôle plus important que le CO2 dans la structure de l’écosystème notamment en favorisant la croissance des bactéries d’affinage et sur l’orientation des voies métaboliques. 2,5% d’oxygène et 10% de CO2 dans l’environnement des fromages seraient nécessaires pour obtenir des fromages aux caractéristiques microbiennes, biochimiques proches de celles des fromages en pain entier sous aluminium. Par contre, ces fromages différaient du point de vue sensoriel par l’aspect visuel et une texture en bouche plus pâteuse. Cette étude apporte des connaissances sur l’effet de l’environnement gazeux sur l’écosystème fromage pour la définition d’un cahier des charges pour la conception d’emballages adaptés aux fromages à pâte persillée
This thesis aims to characterize the interactions between atmospheres, microbial communities and matrix to help to develop packagings adapted to the storage of blue veined cheese; Bleu d’Auvergne and Fourme d’Ambert. In this purpose the effect of commercial packaging and different atmospheres on respiratory activities and on the evolution of microbial, biochemical sensorial characteristics of these two cheeses was evaluated. Cheese pieces stored under vacuum with films more or less permeable to O2, CO2, water vapour distinguished from the whole cheese under aluminium foil by lower level of ripening bacteria (Staphylococcus, Arthrobacter, Brevibacterium) and pH associated with low ammonia production. They were also characterised by higher level of ketones due to their lower degradation, secondary alcohols, esters and lower content in free fatty acids and compounds from branched chain amino acids. They were perceived more pungent, less bitter and moister than those in aluminium foil. Experiments with Bleu d’Auvergne cheeses incubated in controlled atmosphere of O2, CO2, have shown that O2 play a more important role than CO2 to govern the structure and the activities of cheese ecosystem. 2.5 % of O2 and 10% of CO2 in the cheese environment should be necessary to obtain microbial and biochemical characteristics close to those of whole cheese under foil. Nevertheless, their visual aspect and their texture (smooth in mouth) were quite different than those of whole cheeses. This study provides new knowledge about the effect of gaseous atmospheres on the cheese ecosystem that will be useful to define new packagings
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7

Turesson, Joakim, and Filip Ivarsson. "Trådlös dynamisk lastbalansering." Thesis, Högskolan i Halmstad, Akademin för informationsteknologi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-44738.

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The purpose of this project was to create a gateway between a Z-Wave device and a Bluetooth device.The gateway is supposed to make it possible for the company AES AB to sell and install a dynamic load balancer to their EV charger, even after it’s already been installed. The method that was used during this project was LIPS, where the project where divided into multiple subtargets. Each subtarget then had it’s own test which is shown in the results. The results for the overall project and not just the subtargets, are that a concept solution was created. The concept solution was able to forward the Z-Wave devices values to the Bluetooth device that was the target, but did so using a Raspberry Pi and a Z-Wave stick instead of a Z-Wave transceiver. The conclusion for the bachelor thesis is that the concept solution worked, and the demands of the assignment was fullfilled.
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Fabre, Jean-François. "Etude in vitro et in silico de l'extraction, modification chimique et assemblage de phospholipides pour la construction de nouvelles vésicules végétales : exemple de la caméline." Thesis, Toulouse, INPT, 2019. http://www.theses.fr/2019INPT0066.

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Анотація:
Dans le cadre de plusieurs projets de recherche impliquant des partenaires académiques, industriels, et centres techniques locaux et nationaux (CLE Région n°14050981 : SMON-FERT, Réseau Carnot 3BCAR : CAMELINOIL, ITE PIVERT n° 2015-GENESYS-WP3P18-063 PROBIORAF, FEDER Convention N°16004168 : CHIA-EASYNOV), des approches d’études génériques de schémas intégrés ont été conçues pour valoriser le mucilage et les phospholipides de graines oléoprotéagineuses à mucilage, prenant ici l’exemple de la caméline. Présent à la surface des graines, le mucilage peut être efficacement extrait par l'utilisation d’une colonne à ultrasons alliée à des conditions de cisaillement adéquates. Sa composition et les propriétés qui peuvent en découler (comportement viscoélastique notamment) dépendent fortement des conditions de son extraction, en particulier du temps de traitement, comme le révèle le suivi continu de plusieurs paramètres (couple moteur, pH, conductivité, indice de réfraction). Riche en omega-3, l’huile est ensuite extraite des graines entières ou démucilaginées par pressage monovis pour les besoins alimentaires et de l’oléochimie dans ces différents programmes. Les phospholipides peuvent ensuite être récupérés par un traitement ultrasonore de l’huile en présence d’une faible quantité d’eau. Selon une méthode colorimétrique mise au point et des analyses chromatographiques, il apparait que si le rendement d’extraction d’huile dépend du taux d’humidité des graines, l’huile extraite contient peu de phospholipides, ceux-ci se retrouvant principalement dans le tourteau. Etant démucilaginé, ce dernier peut se prêter plus facilement à un traitement aqueux. Cependant, l’activation d’enzymes dans ce milieu impose le recours à des solvants organiques. Un couple de solvants comme l’éthanol et l’acétate d’éthyle est utilisé en remplacement du couple traditionnel méthanol/chloroforme, donnant des rendements similaires avec un impact supposé bien moindre sur la santé, l’environnement, de même que sur les possibilités de valorisation du tourteau. La composition des phospholipides détermine en grande partie les propriétés des vésicules qu’ils peuvent former en milieu aqueux. Pour élargir ces propriétés, notamment dans le domaine de l’encapsulation d’éléments nutritifs ou éliciteurs pour les plantes dans le programme SMON-FERT, une modification chimique des phospholipides a été entreprise. L’utilisation de faibles concentrations (<0,1 mM) de bleu de méthylène, en présence d’air et sous illumination (pour la génération de l’oxygène singulet), permet l’obtention d’hydropéroxydes à partir de phospholipides modèles (lécithine de soja) dissous dans l’éthanol. La variation de la concentration en photosensibilisateur et du volume d’air disponible permet de moduler le rendement d’oxydation des phospholipides pour aboutir à des vésicules de différentes tailles, charges et perméabilités ioniques. Si des conditions oxydantes trop poussées aboutissent à la perte des propriétés d’encapsulation, des conditions modérées permettent l’obtention de vésicules plus petites avec une perméabilité accrue de l’eau et de KCl. La dynamique moléculaire en mode « tout atome » et « gros grains » permet de relier cette perméabilité accrue à une diminution de l’épaisseur de la membrane, une augmentation de l’aire occupée par phospholipide et une plus grande hétérogénéité des vésicules formées. La combinaison d’outils in vitro et in silico peut ainsi permettre de comprendre comment adapter la modification chimique des phospholipides aux propriétés fonctionnelles voulues
Within the framework of several research projects involving academic and industrial partners as local and national technical centers (CLE Region n°1405050981 : SMON-FERT, Carnot network 3BCAR : CAMELINOIL, ITE PIVERT n° 2015-GENESYS-WP3P18-063 PROBIORAF, FEDER Convention N°16004168 : CHIA-EASYNOV), generic studies of integrated schemes have been designed to valorise mucilage and phospholipids from mucilaginous oilseeds, taking here the example of camelina. As the outermost surface layer of the seeds, mucilage can be effectively extracted by using an ultrasonic reactor with adequate shear conditions. Its composition and derived properties (as the viscoelastic behaviour) strongly depend on the parameters of its extraction, in particular the processing time, as revealed by the continuous monitoring of several responses (motor torque, pH, conductivity, refractive index). The omega-3 rich oil is then easily extracted from whole or demucilaginated seeds by single screw pressing for food and oleochemical needs in these different programs. According to chromatographic analyses and a specifically developed colorimetric method, it appears that if the oil extraction yield depends on the moisture content of the seeds, the phospholipid extraction yield remains low, these polar compounds being mainly found in the meal. Phospholipids can be recovered from the extracted oil by ultrasound-assisted precipitation with the presence of a small amount of water. Concerning the meal, as it is mucilage-free, it can be easily treated with water. However, the activation of enzymes in this solvent encourages the use of organic ones. A couple of solvents such as ethanol and ethyl acetate is used to replace the traditional methanol/chloroform mixture, giving similar yields with a supposedly much lower impact on health and environment, as well as on the potential further use of the meal. The composition of phospholipids largely determines the properties of the vesicles they can form in an aqueous medium. To extend these properties, particularly in the field of encapsulation of nutrients or elicitors for plants in the SMON-FERT program, a chemical modification of phospholipids has been undertaken. The use of low concentrations (<0.1 mM) of methylene blue, in the presence of air and under illumination (for the generation of singlet oxygen), allows the production of hydroperoxides from model phospholipids (soy lecithin) dissolved in ethanol. Using two variables (photosensitizer concentration and air volume) response surface methodology allows the oxidation to be modulated to produce vesicles of different sizes, charges and ionic permeability. If excessive oxidizing conditions result in the loss of encapsulation properties, moderate conditions allow the production of smaller vesicles with increased water and KCl permeability compared to unoxidized vesicles. Molecular dynamics study in "all atom" and "coarse grain" modes makes it possible to link this increased permeability to a decrease in membrane thickness, an increase in the area per lipid and a greater heterogeneity of the vesicles formed. The combination of in vitro and in silico tools can thus provide a way to adapt the chemical modification of phospholipids to the desired functional properties
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Lablanche, Pierre-Yves. "Study of the dynamics of barred early type galaxies via numerical simulations." Phd thesis, Université Claude Bernard - Lyon I, 2012. http://tel.archives-ouvertes.fr/tel-00943337.

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Since the 30's and Edwin Hubble's famous classification, galaxies are usually separated in twogroups : the late-type galaxies (LTGs) and the early-type galaxies (ETGs). The LTGs family ismainly made of spiral galaxies (S) while the ETGs family is composed of elliptical (E) and lenticular(S0) galaxies. A morphological study of all these galaxies revealed that around 60% of LTGs and45% of S0 present a bar. It has also been shown that, in the local Universe, galaxies fall into twobig groups : the blue cloud mostly populated by LTGs and the red sequence mainly made of ETGs.Several mechanisms are responsible for this distribution and the secular evolution is obviously animportant one to examine, sepcially in the context of bars, as an important number of studiesshowed the importance of bars in the dynamics and evolution of a galaxy.The goal of my thesis is to study the importance of the formation and ensued bar-drivenevolution influence on ETGs evolution. In that context, I have performed N-body simulations ofbarred (and unbarred) galaxies in order to investigate the following issues.First of all, I focused on the influence of a bar in a galaxy when modelling it with a dynamicalmodel assuming an axisymmetric mass distribution. As these kinds of models allow to determine themass-to-light ratio M/L, thus the dynamical mass of an observed galaxy, but also its inclinationand its anisotropy, it is important to evalute the consequence of the presence of a bar on theseparameters. I have shown that, depending on the galaxy inclination and the bar position angle,M/L is most of the time biased and overestimated, and this can be up to 25%. The size andstrength of the bar also seem to be important factors but a deeper study has to be done to quantifythis preliminary result.In a second step, I have studied the role of bars on the mass and metallicity redistributionsin a lenticular galaxy. I confirmed that the presence of a bar, due to its influence on its hostsystem dynamics, flattens pre-existing metallicity gradients. Moreover, I showed that the degree offlattening and the position of affected regions are directly correlated with the bar structure and thelocation of the dynamical resonances. Nonetheless, this dynamical effect cannot explain the varietyof observed ages and metallicity gradients. The consequences of a barred gravitational potentialon the gas dynamics and the stellar formation should therefore be investigated. This is the topicof the last set of numerical simulations produced which will allow to better understand the globalinfluence a bar has on the secular evolution of ETGs.
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Zhang, Huaijian. "Boundary Integral Techniques in Three Dimensions for Deep Water Waves." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1306712208.

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Частини книг з теми "Bleb Dynamics"

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Molander, Sören. "“Blob” analysis of biomedical image sequences: A model-based and an inductive approach." In Analysis of Dynamical and Cognitive Systems, 169–87. Berlin, Heidelberg: Springer Berlin Heidelberg, 1995. http://dx.doi.org/10.1007/3-540-58843-4_18.

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Pałka, Dariusz, Robert Lubaś, Giuseppe Vizzari, and Jarosław Wąs. "Comparison of the Use of UWB and BLE as Positioning Methods in Data-Driven Modeling of Pedestrian Dynamics." In Parallel Processing and Applied Mathematics, 492–501. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-30445-3_41.

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Fam, Jonathan M., Gadi Wollstein, and Joel S. Schuman. "The role of ocular inflammation, fluid dynamics, and bleb morphology in surgical outcomes." In The Science of Glaucoma Management, 287–303. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-88442-6.00032-7.

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Vorotović, Goran, Nebojša Petrović, Časlav Mitrović, and Vesna Šešum-Čavić. "Possibilities of BLOB (Binary Large OBject) and CLOB (Character Large Object) Integration Into the Core of IoT and Using the SQL Platform for Distributing a Large Amount of Data to HTML, JAVA, and PHP Platforms." In Emerging Trends and Applications of the Internet of Things, 235–76. IGI Global, 2017. http://dx.doi.org/10.4018/978-1-5225-2437-3.ch009.

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This chapter identifies and describes the key concepts and techniques for BLOB and CLOB integration into the IoT core. Data system centralization has sped up the solution of problems with large amounts of data storage and processing, particularly if the data is large by its nature. In that sense, everyday stream of photos, audio and video content, large textual data files led to new concepts BLOB and CLOB. Adequate examples of stored procedures, views, C#, JAVA, HTML5 i PHP languages, follow the establishing communication methods. Finally, the chapter will illustrate two practical examples of IoT: the example for pagination on a large database with million BLOB and CLOB objects, and the example for dynamic mechatronic system of a fire truck with feedback.
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KIENLE, DIEGO, ROLAND RZEHAK, and WALTER ZIMMERMANN. "-SHELL BLOB MODEL INSPIRED BY SIMULATIONS OF TETHERED POLYMERS." In Molecular Dynamics On Parallel Computers, 325–28. WORLD SCIENTIFIC, 2000. http://dx.doi.org/10.1142/9789812793768_0026.

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Braschi, G., G. Danese, I. De Lotto, D. Dotti, M. Gallati, F. Leporati, and M. Mazzoleni. "Vortex blob models implemented on a parallel special purpose computer." In Parallel Computational Fluid Dynamics 1996, 297–304. Elsevier, 1997. http://dx.doi.org/10.1016/b978-044482327-4/50104-2.

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Wuthnow, Robert. "Recovering from the Great Depression." In Remaking the Heartland. Princeton University Press, 2011. http://dx.doi.org/10.23943/princeton/9780691146119.003.0003.

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This chapter examines how the Middle West recovered from the ill effects of the Great Depression. The Great Depression was something Americans hoped they would never experience again. In the rural Midwest, foreclosures and sheriff's auctions were common. The worst drought years devastated the land. Dust storms blew with such intensity that crops failed and machinery broke down. World War II sparked the economy, revived agriculture, and coincided with better weather. However, the war took millions of men and women away from their families, necessitated mandatory rationing, and drove up prices. When it was over, rural communities faced continuing challenges. The chapter considers the case of Smith Center, Kansas, to illustrate the challenges rural communities faced as they overcame the setbacks of the Great Depression and prepared for the era ahead. Recovery from the Great Depression varied across middle America, but many of the dynamics evident in Smith County occurred elsewhere.
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"scto th ara ti rsetc ic te s d ( M mo OdSe ) l . f S or m ec it ahstadnad ta are called model output 1994). The state of ENSO is only one of many factors obs terevveedn SS gi T v en a perfect SRSoTp el feow re sckais ( t 1 -9 9i7 .e ) ., shtohweu (K se r d is hn to aKfuom re acrae st t al t . h1e99I5n ) d . ian summer monsoon m to oid ts elEuNsS ed O -a r t -N t C he E P cu rrent operational atmospheric Ward et al. (1993) discuss the useful level of skill In their study e , la ftoerd ty r -a fi i need vne fa lylspastu te brsn ta snfto ia rlpa ra d c ju ti sctamleun se ts . m of a d tr eop in ic a re l al n o ti r m th e A by f ri tchaenU fo KreM ca e st tse or th ol aotgihcaavleOb ff eiecnee (h xianm dc in aesd ts ) fo bra se th d e on specif e ie a d rs ( oofbsseeravseodn ) alSS fo Trew ca esrtess re in la ctei on 1s9h8i6p . swTih th esgeloabrael ly ladrigsetlry ib ubtaesdedSSoTn , SS st Tatiin st itchaelm ea o ch d el sism imulation wapserrieopde at 1e9d50 th -i 9r4 te . enEa ti cmh es s , e w as hoenraelpSoonue th n t. A O tla vnetrict , hean la dstade re claad ti e v , eltyhesm re aalll -ti EmNeSfOo re ccoam sts ­ u initial co unldaitt io io nnswatso st easrttiemdatwe ith th e s lig mha tl gynid tu if dfee ren otfvhaalviedah te addahisnkdic ll asstismim lar adteotwhiatthotbhtea in seadme fr osm ta t c is rto ic sasl -v av anrp ia re ti doincs ta ble and therefore random atmospheric methods. The chief limitation to further progress is a rai enrfaag ll edf or tion th ca elcm ul oad te e l. thTehebtehsitr te meondseilmeus la ti t m io antseao re flsaecaksoonf ( gJluonbeal th tr roopu ic gahlSSeSpTtefm or beecra ) s . tsAvcaclu id ra f te orfo th re ecawse ts ttgylpoibcaall ly v , a a ri l th ea ocuhghgitvheen seasonal SST pattern. Quite of ENSO would help, but would in most years be well, it sho at w io ends in sem as oodneall re ra piln ic fa altledpa th tt eeronbs se qruvietdeifn al sluffo fi rceiceanst. tsAatrmeoaslp so heu ri scedd , ynbaumtitchaely -m oondleyl -h baavseedusreaf in ul ­ T re hgu io s ns fo rofptrheec ip w it s a ystematic or tlido , n -t rhee la tmesdpaa ti pap ll l y ic var odel pre a d ti i o ct nys in g in biases. skill with lead times of a month or less before the require some kind ions wmoaun ld y r th ai antf al hlusm ea asnoin ty ( Fhoa ll sanidncerteaals . ed1 99 th 1 e ). Istuisscaelpsto ib p il o it s y si b o le f g ki ennderoaf te bdi as byadajuM stm OS ofteacdhjnuisqtumee . n T ts h , e perhaps those tropical west Africa to drought through the pro­ production of operat einot places a huge bnue rd ed en foorntthhiesg co re vsesrivoevreerdu re c c ti eonntd in ecnaedaers -. coTahsitsalcw ha e n st geAfirn ic a th nefolraensdtM because each time the m na oldd el y n is a m ch ic aanlge fo d r , eacansetwmsoedteo ls f s (e u . r g fa ., ceElb ta ohuinrdaan ry dhGaosnbgee1n99s6 ho ) w to nhbayve se v th e e ra lpo au te tnhtoirasla ne O ed S e statistics must be calculated to provide the to weaken the north African monsoon. More studies nneacleys si d sa s ad ojfu ry Msm tm u e lt n id ts e . c T ad h a is l re en qu se ir m es bltehsetgoe ne orbatta io in natnhdetorfopp ic oasls ib nloer th re g A io fr n ic aalnarnatihnrfo al p l, ogaesniwce ll inafs lu efnocr es on fundamental OiSmp st rao ti vse ti m cs enatnsdtuond th eerssecom re osdtehles , nseuecdh fo as r reg W ion ar sdwe it thaml. a ( r 1 gi 9n9a3l ) seaalssoonadlisrcau in sfsaltlh , e a re sk n il eleo de odt . her tahboosvee . related to the flux adjustments, discussed m tim ad eefboy re c th a e st sff real­ UK orM th et eeon ro olro th g e ic aasltOBfrfaizcielsw in ectes1e9 as 8o7n . dro Kungohwt le p d re gdeico ti fonEsNS in O m is a n in ysup ff airctisenotffotrher eg w io o n rl adlT se hviesreisa relatively dry area, subject to intermittent lbeescsaium se p o in rt a th n ese regions its influence is either small or Hastenr dartohuagnhdt. c T ol hleasbeorfao to re rs c a ( set . s g , ., asHw as etlelnarsatthho1s9e95b ) y , p ea ro st foBurnad zil an t than other factors. For instance, north­ are mainly statistical, although real-time dynamical patterns ( lFyo ll iandf lu west A nd eentcael. d frican w 19 b9y1 , H tro ept ic saela so Ant la ra nitn ic fa ll astenrath 1995, WSaSrTeM for eetceaosrtosl og hiacvae ard based on tropi lcbaOlAfefe ic n e . mTahdee st saitn is ctei cal 19f9o4 re ca bsy ts tahreeS1S9T 97 ) i . n In th aeddtirtoipoinc , aplaIrn ts d i of the Sahel are affected by on ENSO SSTs. On tl aanv ti ecraSgSeT , t a ro npoim ca allyAp tl aatn te ti rcnsSaSnTdsF Si o m ll i a la nrd ly , eltocaall . SS 19 T9p1a , tt Bar anns to Onceaannd (P Sam lm ith er11999866 ). , h re agvieonaboofuSt ou tw th ic Aem th e e ri cian , fl aule th nocue gh ofexEtN re SmOeE fo NrStOhs is , A no urstth ra w li eas , tnooftA ab ulsyt ra ilniatih er e ns influence precipitation in such as that in 1982-3, can dominate the circulation Drosdowsky 1993, F n re d th IendA ia uns tr O al c ia enanw north and and precipitation patterns over tropical South ericksen and i B nt aelrgo ( v e. ign . d , A sis m te e n ri t c ly a . h T ig hhe le r v ea el l -t o im f e sk iflol, resc im as itlsarhtaovethhaatdobatac in oend ­." In Droughts, 63. Routledge, 2016. http://dx.doi.org/10.4324/9781315830896-43.

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Тези доповідей конференцій з теми "Bleb Dynamics"

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Sharma, Puja, Kevin Sheets, and Amrinder S. Nain. "The Influence of Polymeric Fiber Stiffness and Alignment on Cytoplasmic Bleb Dynamics and Migration of Glioblastoma Multiforme Cells." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80923.

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Cell migration is a tightly regulated phenomenon necessary for regular physiologic processes such as wound healing, immune response, embryonic development, growth, and regeneration [1–3]. Consequences of abnormal migratory behaviors include autoimmune diseases and metastasis during cancer progression [4, 5]. Described as one of the hallmarks of cancer, metastasis is a complex multistep process, and is responsible for 90% of cancer deaths in humans. A better understanding of the process of metastasis is of paramount importance in developing efficient cancer treatment therapies and drugs [6].
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Randive, Pitambar, Amaresh Dalal, and Partha P. Mukherjee. "Simulation of Blob Dynamics Inside a Channel Under Acoustic Excitation." In ASME 2013 Heat Transfer Summer Conference collocated with the ASME 2013 7th International Conference on Energy Sustainability and the ASME 2013 11th International Conference on Fuel Cell Science, Engineering and Technology. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/ht2013-17372.

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The displacement of a three-dimensional immiscible blob subject to oscillatory acoustic excitation in a channel is studied with the Lattice Boltzmann method. The effects of amplitude of the force, viscosity and frequency on blob dynamics are investigated. The trend for variation of mean displacement of blob and frequency response is in agreement to that of the previous two-dimensional studies reported in literature. The response of the blob with pinned contact line shows underdamped behavior. It is also found that increasing the amplitude of the force increases the mean displacement and frequency response.
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Sahoo, G., R. Paikaray, S. Samantaray, P. Das, J. Ghosh, M. B. Chowdhuri, and A. K. Sanyasi. "Origin and dynamics of plasma blob." In 2015 IEEE International Conference on Plasma Sciences (ICOPS). IEEE, 2015. http://dx.doi.org/10.1109/plasma.2015.7179896.

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4

Kemena, Tineke, Rogier van de Wetering, and Rob Kusters. "The impact of IT human capability and IT flexibility on IT-enabled dynamic capabilities." In 32nd Bled eConference Humanizing Technology for a Sustainable Society, Bled, Slovenia, Conference Proceedings. University of Maribor Press, 2019. http://dx.doi.org/10.18690/978-961-286-280-0.29.

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Lele, Ashish K., Balaji V. S. Iyer, Vinay A. Juvekar, Albert Co, Gary L. Leal, Ralph H. Colby, and A. Jeffrey Giacomin. "Blob-Spring Model for the Dynamics of Ring Polymer in Obstacle Environment." In THE XV INTERNATIONAL CONGRESS ON RHEOLOGY: The Society of Rheology 80th Annual Meeting. AIP, 2008. http://dx.doi.org/10.1063/1.2964723.

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Qu, K. Y., and Y. Jiang. "STUDIES ON DYNAMIC ICE-MAKING BY SUPERCOOLING WATER." In Thermal Sciences 2000. Proceedings of the International Thermal Science Seminar Bled. Connecticut: Begellhouse, 2000. http://dx.doi.org/10.1615/ichmt.2000.thersieprocvol2thersieprocvol1.510.

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De Schepper, Tom, Alexander Vanhulle, and Steven Latre. "Dynamic BLE-based fingerprinting for location-aware smart homes." In 2017 IEEE Symposium on Communications and Vehicular Technology (SCVT). IEEE, 2017. http://dx.doi.org/10.1109/scvt.2017.8240316.

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Boric, Miran, Rebeca P. Diaz Redondo, and Ana Fernandez Vilas. "Dynamic Content Distribution over BLE iBeacon Technology: Implementation Challenges." In 2018 5th International Conference on Control, Decision and Information Technologies (CoDIT). IEEE, 2018. http://dx.doi.org/10.1109/codit.2018.8394958.

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Qu, K. Y., and Y. Jiang. "Abstract of "STUDIES ON DYNAMIC ICE-MAKING BY SUPERCOOLING WATER"." In Thermal Sciences 2000. Proceedings of the International Thermal Science Seminar Bled. Connecticut: Begellhouse, 2000. http://dx.doi.org/10.1615/ichmt.2000.thersieprocvol2.600.

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Lee, Taeseop, Jonghun Han, Myung-Sup Lee, Hyung-Sin Kim, and Saewoong Bahk. "CABLE: Connection Interval Adaptation for BLE in Dynamic Wireless Environments." In 2017 14th Annual IEEE International Conference on Sensing, Communication, and Networking (SECON). IEEE, 2017. http://dx.doi.org/10.1109/sahcn.2017.7964929.

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Звіти організацій з теми "Bleb Dynamics"

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Blinov, Sergey, Nathan Mackey, Ari Le, and Adam Stanier. Dynamics and Instabilities of a Plasma Blob in Curved Magnetic Geometries. Office of Scientific and Technical Information (OSTI), August 2022. http://dx.doi.org/10.2172/1883106.

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Yu, Haichao, Haoxiang Li, Honghui Shi, Thomas S. Huang, and Gang Hua. Any-Precision Deep Neural Networks. Web of Open Science, December 2020. http://dx.doi.org/10.37686/ejai.v1i1.82.

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We present Any-Precision Deep Neural Networks (Any- Precision DNNs), which are trained with a new method that empowers learned DNNs to be flexible in any numerical precision during inference. The same model in runtime can be flexibly and directly set to different bit-width, by trun- cating the least significant bits, to support dynamic speed and accuracy trade-off. When all layers are set to low- bits, we show that the model achieved accuracy compara- ble to dedicated models trained at the same precision. This nice property facilitates flexible deployment of deep learn- ing models in real-world applications, where in practice trade-offs between model accuracy and runtime efficiency are often sought. Previous literature presents solutions to train models at each individual fixed efficiency/accuracy trade-off point. But how to produce a model flexible in runtime precision is largely unexplored. When the demand of efficiency/accuracy trade-off varies from time to time or even dynamically changes in runtime, it is infeasible to re-train models accordingly, and the storage budget may forbid keeping multiple models. Our proposed framework achieves this flexibility without performance degradation. More importantly, we demonstrate that this achievement is agnostic to model architectures. We experimentally validated our method with different deep network backbones (AlexNet-small, Resnet-20, Resnet-50) on different datasets (SVHN, Cifar-10, ImageNet) and observed consistent results.
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