Дисертації з теми "Biopsie – analyse"
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DUMAZER, PHILIPPE. "La biopsie du rein : analyse critique des indications, complications et resultats a propos d'une serie consecutive de 1000 biopsies renales." Toulouse 3, 1988. http://www.theses.fr/1988TOU31302.
Повний текст джерелаDOP, EPRINCHARD MIREILLE. "Analyse de 285 ponctions biopsies renales percutanees : resultats et apport de l'echographie." Nice, 1989. http://www.theses.fr/1989NICE6577.
Повний текст джерелаHunckler, Franck. "Analyse épidémiologique rétrospective des biopsies rénales en Guadeloupe sur une période de 20 ans (1974-94)." Saint-Etienne, 1995. http://www.theses.fr/1995STET6236.
Повний текст джерелаKarnoukian, Marc. "Imagerie spectro-polarimétrique : système, algorithmes et biopsie optique." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAD001/document.
Повний текст джерелаCancer is a pathology that must be detected as soon as possible in order to increase the chances of recovery. These studies investigate the contribution of polarimetric signature to the characterization and identification of cancerous tissues. It is a matter of extracting multidimensional polarization images of the physical information which characterize the constituents of the object well beyond the visual information of the intensity images. During this thesis, a Mueller imager, POLARIS, was set up, as well as the appropriate processing and calibration tools. A method of Mueller images segmentation in non-homogeneous illumination has been proposed. A first database of polarimetric multi-spectral images of healthy and pathogenic tissues in mice was constructed. An original approach was finally proposed based on random forests to extract from a set of physical parameters a set of parameters allowing to differentiate the healthy zones from the pathogenic zones at different working wavelengths. A comparison is proposed with the literature and validates the approach
Schilling, Anne. "Die Endometriumbiopsie bei der Stute- eine Analyse der histologischen Befunde zwischen 1992- 2012 am Leipziger Institut für Veterinär- Pathologie." Doctoral thesis, Universitätsbibliothek Leipzig, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-224653.
Повний текст джерелаFahr, Florian. "Zeitliche und räumliche Analyse histomorphologischer Befunde aus Eigennierenbiopsien im Raum Leipzig über einen Zeitraum von 20 Jahren." Doctoral thesis, Universitätsbibliothek Leipzig, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-216027.
Повний текст джерелаBackground and Objectives: Annually, 4% of Western Europe\'s population fall ill with acute kidney injury (AKI). Furthermore, 8.5% of the same population are affected by chronic kidney disease (CKD). In Germany, valid nationwide epidemiological data on renal pathology didn\'t exist at the time of this study, although progress has been made with creating the German kidney biopsy register. This study analyzes temporal and spatial variances in the histomorphological spectrum of renal diseases of native kidney biopsies in the metropolitan area of Leipzig, Germany, from 1993 through 2012. Methods: For this study, a temporally and spatially structured kidney biopsy register was created from nephro-pathologic biopsy results. Spatial analysis was implemented by giving every entry its corresponding postal code. Unidentifiable entries were omitted. If the postal code was determined for every case within a timeframe, incidences for the city of Leipzig were calculated for the timeframe. Incidence for the surrounding areas were not calculated, because coverage was expected to be incomplete. Statistical analysis was done via Chi-Squared-Test or analysis of variances. Results: For this study n=943 cases were analyzed, omitting pediatric and follow-up biopsies. The leading diagnosis was IgA nephropathy (IgANP) with 19.5% (male: 22.1%, female: 15.4%), followed by hypertensive nephropathy (HTNP) resp. focal-segmental glomerulosclerosis (FSGS) and granulomatosis with polyangiitis (GPA). Spatial variance between the analyzed regions was high. Compared to the city of Leipzig, IgANP was observed one third more frequently in the surrounding regions. High temporal variance was also observed. From 2009 through 2012, HTNP/FSGS became leading diagnosis with 18.9%, followed by GPA with 17.8% and IgANP with 15.6%. Furthermore, frequency of thin base membrane disease (TBMD) resp. Alport\'s syndrome decreased sharply. Variance in spatial distribution was also observed over time. On the basis of determined postal codes, incidences for the city of Leipzig were calculated for the years 2001 through 2009. Highest annual incidence was observed in GPA with 0.9 (0.0-2.2) cases per 100 000 people, followed by HTNP/FSGS with 0.8 (0.2-2.2), IgANP with 0.8 (0.2-1.4). Conclusions: The spectrum of kidney pathology for the metropolitan area of Leipzig is in accordance with the data in literature, as far as consolidated figures were available. Results in compared studies were highly heterogenous. Some differences, e.g. decrease in TBMD resp. Alport\'s Syndrome or fluctuation of DNP, can be attributed to variance in indication for biopsy. External factors were discussed
Liuu, Sophie. "Analyse protéomique ciblée à haute résolution : un outil puissant pour le diagnostic clinique à partir de prélèvements de tissus amyloïdes bruts." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066373/document.
Повний текст джерелаAmyloidosis is rare disease that appears as an insoluble deposition of specific extracellular proteins. Thirty classes of amyloidosis have been reported and their diagnosis relies on the identification of the associated proteins by immunohistochemistry analysis of the affected tissues. However, its interpretation is highly dependent on the pathologist and can be ambiguous. Laser capture micro-dissection (LCM) and mass spectrometry are a promising alternative. We have developed a targeted proteomics approach from raw tissues treated with ultrasound. We showed that the biopsies taken from patients presenting different classes of amyloidosis can be proteolysed by ultrasonic treatment. This technique increases the efficiency and reduces the time of digestion (90s instead of 15h), while minimizing the amount of material needed. After a first unbiased proteomics study for clinical diagnosis of patients with amyloidosis in various organs/tissues (kidney, lung, salivary glands, testicle, vitreous humor and spleen), we have optimized a dedicated screening method for a technological transfer to the French clinical departments. For this, two methods show very promising results: SRM (selected reaction monitoring) on a low-resolution mass spectrometer, available in some clinical laboratories, and PRM (parallel reaction monitoring) available on the latest generation of high-resolution instruments which provide fast results. In conclusion, we propose here a specific protocol for a robust diagnosis and rapid classification of amyloidoses
El, Sissy Carine. "Analyse de la composante immunitaire des cancers colorectaux et de son impact potentiel sur les stratégies thérapeutiques." Electronic Thesis or Diss., Sorbonne université, 2021. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2021SORUS404.pdf.
Повний текст джерелаIn this work, I analyzed the quality of the immune infiltrate of locally advanced rectal cancer (LARC) and showed that an adaptation of the Immunoscore test to diagnostic biopsies (ISB: tumor infiltration in T cells CD3+ and CD8+) was feasible. In two cohorts (n1=131, n2=118) of LARC patients treated with neoadjuvant radiochemotherapy (nRCT) before surgery, I observed that the quality of the immune infiltrate, assessed by ISB, had a prognostic value in terms of recurrence-free survival [HR= 0.21; 95%CI 0.06-0.78, P=0.009], and was predictive of the quality of the response to nRCT (P<0.001). Tumor transcriptomic and protein analyses showed heterogeneity in local immune response to nCRT. A significant increase in the expression level of genes involved in cytotoxicity (GZMA, GZMH, GZMK, PRF-1), Th1 response (TBX21, STAT4), activation (CD69) and inhibitory costimulation (CTLA-4, LAG3) was observed in responder patients. The post-nCRT immune activation status correlated with the initial ISB. ISB coupled with post-nRCT imaging improves the prediction of histological complete response to nRCT, and thus the selection of patients eligible for an organ preservation strategy (Watch&Wait, W&W). In 2 independent cohorts of W&W patients, a High ISB predicted a low risk of recurrence at 5 years (3%; CI95% 0-10%). Finally, we showed that nRCT responder patients could show evidence of adaptive T and B immune stimulation, highlighting the immune benefit of organ (and draining lymph node) preservation in the nRCT response setting
Orczyk, Clément. "Modélisation du cancer de la prostate par l'imagerie : détection, stratification, planning thérapeutique et suivi en 3D d'une thérapie focale basés sur le recalage-fusion d'image en multi modalité." Thesis, Normandie, 2017. http://www.theses.fr/2017NORMC405/document.
Повний текст джерелаConventional prostate MRI, enhanced by diffusion and perfusion sequences, and then named multiparametric, showed high performances for detection of prostate cancer using visual scoring. Indications in stratification, prognosis, treatment planning and follow up are currently under investigations.First part of this work attached itself to describe, elaborate and use a non-rigid image fusion method in 3D between gold standard histology of radical prostatectomy and MRI. Investigations captured the significant differences in shape and volume of in vivo and ex vivo prostate using MRI. The developed multimodality fusion method was applied to a cohort of patients who underwent MRI prior surgery. Results showed a stratified underestimation of cancer volume by MRI. Clinical output resides in detection, stratification and surgical planning.The second part proposed some texture analysis of sequences and quantitative maps. As a multiparametric approach, the Entropy Score is applied in a pilot cohort at time of biopsy and showed some potential usefulness to select MRI targets without compromising detection of significant cancer. By positively correlating with the Gleason Score and the maximal core length of cancer, Entropy Score participates of stratification of cancer.The third part explored application of image registration in the longitudinal follow up of an emergent therapy, said focal (FT). As a conservative approach, FT induces very local deformation of the gland which appears to be appropriately modelled by non-rigid registration, then opening possibilities to guide further control biopsy and radiologic assessment
Bonnel, David. "Développements et applications d'approches protéomiques pour la recherche de cibles du cancer de l’ovaire et de la prostate." Thesis, Lille 1, 2010. http://www.theses.fr/2010LIL10078/document.
Повний текст джерелаMy PhD’s work has been completely devoted to the optimization and the use of technologies in clinical proteomics, especially MALDI imaging, for research and identification of disease markers. Therefore we have initially developed and applied the tools of statistical analysis based on PCA and hierarchical clustering, called PCA-SDA, which offered an interesting combination with MALDI imaging and allow simplification of data, fine search of molecular changes within the tissue and a classification based on molecular profiles obtained locally on the tissues. Then applied on ovarian cancer biopsies study, this approach allowed us to detect and identify several potential markers playing a role in immune response, adhesion and tumor invasion. However, these mechanisms are known to involve proteases, like proprotein-convertases, in the maturation of various proteins implicated in tumor development. In this context, we studied their expression in prostate cancer. It pointed that only PACE4 was over-expressed in this disease and we were able to establish its role in cell proliferation using in vitro analysis and in apoptotic evasion with the identification of TRPS1, a transcription factor involved in apoptosis, by proteomics approach. So, PACE4 is a potential therapeutic target for cancer due to its leading role in tumor cell capacities
Raoul, Gwénaël. "Plasticité du masseter humain : relation entre les chaînes lourdes de myosine et la dysmorphose dento-maxillo-faciale." Thesis, Lille 1, 2008. http://www.theses.fr/2008LIL10124/document.
Повний текст джерелаMasseter biopsies (161left, 36 right).were performed on 161 subjects undergoing surgical treatment of malocclusion. Patients were classified according to computer-assisted Delaire cephalometric analysis. The 36 patients with both side biopsies were separated into 2 groups: with or without lateral deviation. SDS-PAGE was performed on 28 biopsies to identify myosin heavy chain content. Immunostaining with myosin-isoform-specific antibodies was performed on 197 biopsies to identify 4 fiber types (l, Hybrid, II, NéoAtrial). For each fiber type, percent occupancy and mean area were calculated. Student and Wilcoxon tests were used to compare electrophoresis and immunostaining results from 28 cases, and fibre type compositions on the two sides in 36 patients. An ANOVA test was done to identify correlation between percent occupancy and mean area of each fiber type versus cephalometric classification for aIl 161 left samples. Electrophoresis and immunostaining analysis of slow and fast (lIa & IIx) myosin heavy chains gave equivalent results. Lateral deviation patients showed an increase of type II fiber occupancy (p=0,0286) on the same side as the deviation (short side). Deep bite is associated with an increase of type II fiber occupancy (p=0,0073) and decrease of NeoAtrial mean fiber area (p=0,0401). Class II is significantly associated with an increase in occupancy of hybrid fibers (p=0,0419) and decrease of type II (p=O,0234) as compared with Class Ill. Mandibular position in relation to the skull base trend is associated with an increase of percent occupancy (p=0,0023) and mean area (p=0,0387) of hybrid fibers in case both forward and backward position. CONCLUSION Facial vertical dimension and mandibular lateral deviation is significantly linked to type II fiber percentage occupancy and NeoAtrial mean fiber area. Saggital mandibular position is linked to mean fiber area and percent of hybrid fibers, and percent occupancy of type II fibers
Pesonen, Izabell, and Midia Ismail. "Immunhistokemisk (IHC) analys av låggradigt inflammerade biopsier med apikal parodontit -en pilotstudie." Thesis, Malmö universitet, Odontologiska fakulteten (OD), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-42574.
Повний текст джерелаAim: To investigate the relation between B- and T- lymphocytes in biopsies of root-filled teeth with apical periodontitis with assessed low-grade inflammation. This study will also analyse what the inflammation looks like in relation to the symptoms described in these biopsies. Study design: A pilot study was performed on 10 biopsies of root-filled teeth with apical periodontitis with assessed low-grade inflammation according to the classification system of Danesh et al 2019. The biopsies were collected from Malmö University's biobank. An immunohistochemical staining of antigens CD20+ and CD3+ were performed and analysed by a digital microscope. A comparison of the symptoms from the referrals were performed once the histological results were compiled. Results: There were more T-lymphocytes than B-lymphocytes in 6 of the biopsies. In the remaining 4 biopsies there were an equal amount of B- and T-lymphocytes. The symptoms were not stated for all biopsies in the referrals. Conclusion: According to our pilot study, we see tendencies that T lymphocytes are more than B lymphocytes or that they are equal. No conclusions could be drawn regarding the symptom picture. Larger material from different referrers is required for definitive conclusions. A further staining of CD4 + and CD8 + would be interesting.
Scola, Rosana Herminia 1959. "Estudo dos vacuolos sugestivos de corpos de inclusão citoplasmatica na biopsia muscular : analise clinica, laboratorial, eletroneuromiografica, histoquimica, imunocitoquimica e ultra-estrutural de 16 casos." reponame:Repositório Institucional da UFPR, 2017. http://hdl.handle.net/1884/43840.
Повний текст джерелаContem 33 fots. coladas
Dissertação (mestrado) - Universidade Federal do Parana, Setor de Ciencias da Saude, Programa de Pós-Graduação em Medicina Interna
Resumo: Os vacúolos são formações anormais encontradas nas fibras musculares, podendo ser classificados morfologicamente quanto ao tamanho, número, posição, forma, presença de membranas e se marginados ou não. Os vacúolos marginados, podem ser encontrados em diversas doenças, entre elas a miosite com corpos de inclusão citoplasmática. Esta é caracterizada clinicamente como uma polimiosite crônica tendo na histologia do músculo a presença dos vacúolos marginados, e denominados histologicamente de corpos de inclusão citoplasmáticos, podendo ter filamentos intranucleares e intracitoplasmáticos. Com o objetivo de estudar os vacúolos com aspecto histológico de corpos de inclusão citoplasmática, foram estudados todos os casos entre 1400 biópsias musculares que apresentaram os mesmos, procurando correlacionar com os dados clínicos laboratoriais, afim de verificar a sua especificidade para determinadas doenças. Foram encontrados 16 casos. Dos 16 casos, a idade média foi de 36.0 anos, o início da doença teve a média de 20.5 anos e o tempo de doença de 5.5 anos. Os casos foram classificados conforme a história clínica, hereditariedade, dados laboratoriais, eletrofisiológicos, histoquünicos, imunocitoquímicos e microscopia eletrônica em miosite com corpos de inclusão citoplasmática (4 casos), atrofia muscular espinhal juvenil (6 casos), miopatias distais (3 casos), distrofia de cinturas pélvica e escapular (2 casos) e polineuropatia periférica (1 caso). As enzimas musculares, mais especificamente a creatinoquinase mostrou-se elevada em dez casos. Apenas um caso mostrou moderada redução nas conduções nervosas. A eletromiografia esteve alterada em todos os casos sendo que, em cinco, casos a mesma mostrou sinais de desinervação (ativa e ou crônica), oito foram miopáticos e em dois casos mista (neuromiopática). A biópsia muscular pela histoquímica em cinco casos mostrou histologicamente uma miopatia (ativa e ou crônica); em sete casos, elementos para miopatia e desinervação (misto); em dois casos, desinervação; e em dois casos, miopatia inflamatória. Todos os casos mostravam vacúolos marginados. O estudo imunocitoquímico demonstrou predomínio de linfócitos CD8+ no interstício na maioria dos casos, e ocasionalmente nas regiões perivasculares e no interior das fibras musculares. As miosites por corpos de inclusão citoplasmática tiveram importante aumento de linfócitos CD8+, em relação a outras doenças. A detecção de imunoglobulinas e complemento foram mais evidentes na miosite com corpos de inclusão citoplasmática, embora não demonstre uma diferença marcante, exceto na polineurite em que não teve nenhuma célula ou deposição de imunoglobulina. A microscopia eletrônica demonstrou a presença de filamentos nucleares e dispersos no citoplasma em cinco casos, um caso demontrou a presença de filamentos no núcleo e região subsarcolemal, dois casos mostraram filamentos na região subsarcolemal e citoplasma. Em um caso, os filamentos estavam dispersos no citoplasma e núcleo, sendo que em sete casos não foram observados filamentos intracitoplasmáticos q u intranucleares. Foi concluído que: 1) Os filamentos intracitoplasmáticos e intranucleares não são específicos para uma única entidade; 2) A presença de reação inflamatória auxilia na diferenciação das outras doenças com miosite com corpos de inclusão citoplasmática; 3) A creatinaquinase e eletromiografia não são úteis para diferenciar a miosite com corpos de inclusão citoplasmática das outras entidades; 4) Existe um predomínio de linfócitos T no interstício nas miopatias com corpos de inclusão citoplasmática; 5) Foi notado importante aumento de linfócitos CD8+ no interstício, sugerindo relação com o complexo maior de histocompatibilidade 1 (MCH1); 6) Houve inversão da proporção de linfócitos CD4+/CD8+, sugerindo processo mediado pelo MCH1; 7) As imunoglobulinas e complemento foram detectadas com maior freqüência nas miosites com corpos de inclusão citoplasmática; 8) Os corpos de inclusão citoplasmática ocorrem em diversas entidades, com patogenia e patologia global diferente, sugerindo se tratar de uma reação celular inespecífica, talvez relacionada com tempo de agressão crônica da fibra muscular, tanto nos processos de origem muscular primaria, como de origem neurogênica. VI
Abstract: The vacuoles are abnormal structures of the muscle fibers, who can be morphological classified according the size, number, location, shape, presence of membranes or if they are rimmed. The rimmed vacuoles can be find in several diseases, mainly in the inclusion body myositis. This disease can be presented as chronic polymyositis with rimmed vacuoles in the histology, also called cytoplasmatic inclusion body, with cytoplasmatic or nuclear filaments. With the objectives to study the histopathological aspects of the cytoplasmatic inclusion bodies, we select all the cases who presented rimmed vacuoles among 1400 muscle biopsies, who had the clinical history and laboratory investigation available. We found 16 cases, with mean age of 36.0 years, whose disease started at 20.5 and a mean disease time 5.5 years. The cases where classified regarding the clinical history, hereditary pattern, serum laboratory determinations, electrophysiological tests, histochemical and immunocytochemical analysis and electron microscopic findings in inclusion body myositis (4 cases), juvenile spinal muscular atrophy (6 cases), distal myopathy (3 cases), limb-girdle muscular dystrophy (2 cases) and peripheral neuropathy of unknown etiology (1 case). The serum enzymes, specially the creatinekinases, was increased in ten cases. Only one case had reduced nerve conduction velocity. The electromyography was abnormal in all cases with denervation pattern in five and myopathic pattern in eight and in two cases had a mixed pattern (myopathic and denervation). The muscle biopsy histochemistry had the diagnosis of myopathy (active and chronic) in seven cases, mixed (myopathy and denervation findings) in two cases, denervation in two and inflammatory myopathy in two. All the cases had rimmed vacuoles. The immunocytocheinical analysis showed CD8+ lymphocytes in the interstitial in most cases, occasionally ip the perivascular region and rarely inside the muscle fibers. The inclusion body myositis cases had increased of CD8+ lymphocytes comparing with the other diseases. The immunoglobulins and complement deposition were slight more intense in the inclusion body myositis, comparing with the other diseases. The peripheral neuropathy had no cells or immunoglobulins found any time. The electron microscopy detected filaments in the nucleus and diffusely in the cytoplasm in five cases, one case only in the nucleus and sub-sarcolemmal region, two cases with filaments in the sub-sarcolemmal and cytoplasm. One case had filaments where dispersed in the cytoplasm and nucleus. Seven cases had no filaments found in nucleus or cytoplasm. The following conclusion was drawn: 1) The intracytoplasmic or intranuclear filaments is not specific for only one disease. 2) The inflammatory reaction help in the differentiation of the inclusion body myositis from the other diseases studied. 3) The creatinekinase and electromyography where useless in the differentiation the inclusion body myositis from other diseases. 4) A predominance of T lymphocytes was found in the interstitial tissue in the cases of inclusion body myositis. 5) Was noted an important increased of the CD8+ lymphocytes in the interstitial tissue, suggesting a relationship with the major histocompatibilty 1 complex (MCH1). 6) An inversion of the CD4+/CD8+ lymphocyte's proportion was found , suggesting a mediation by the MCH1. 7) The immunoglobulins and complement deposition were found with major frequency in the inclusion body myositis. 8) The cytoplasmatic inclusion bodies can be found in several diseases with different pathogenesis and pathology, suggesting a non specific cellular reaction, maybe related with the time of the chronic aggression to the muscle tissue, who can be similar in the primary muscle lesion and neurogenic etiology.
CARRIER, JEAN-BAPTISTE. "Cytoponction et microbiopsie echoguidees des masses abdominales : interet de l'association des analyses cytologique et histologique : a propos de 60 observations." Lyon 1, 1989. http://www.theses.fr/1989LYO1M111.
Повний текст джерелаLECRIVAIN, CHIRI FRANCOISE. "Analyse en imagerie par resonance magnetique des osteonecroses de hanches forees." Aix-Marseille 2, 1993. http://www.theses.fr/1993AIX20820.
Повний текст джерелаBovey, D. "The artist biopic : a historical analysis of narrative cinema, 1934-2010." Thesis, University of Westminster, 2015. https://westminsterresearch.westminster.ac.uk/item/9w77z/the-artist-biopic-a-historical-analysis-of-narrative-cinema-1934-2010.
Повний текст джерелаLammers, Christina [Verfasser], and Matthias [Akademischer Betreuer] Paul. "Morphometrische Analyse des prozentualen Anteils und des Verteilungsmusters rechtsventrikulärer Lipomatose in Endomyokard-Biopsien von Patienten mit arrhythmogener rechtsventrikulärer Kardiomyopathie / Christina Lammers. Betreuer: Matthias Paul." Münster : Universitäts- und Landesbibliothek der Westfälischen Wilhelms-Universität, 2012. http://d-nb.info/1027022081/34.
Повний текст джерелаGulley-Stahl, Heather Jane. "An Investigation into Quantitative ATR-FT-IR Imaging and Raman Microspectroscopy of Small Mineral Inclusions in Kidney Biopsies." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1272042834.
Повний текст джерелаLaplatine, Loïc. "Résolution spatiale en microscopie par résonance de plasmon de surface à couplage par prisme." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENY044/document.
Повний текст джерелаPrism-based surface plasmon resonance (SPR) microscopy is an optical imaging technique invented in the late 60s'. Its main advantage lies in its high sensitivity to optical index or thickness variations at a metal surface. Therefore, the monitoring of biological reactions can be performed in real-time without labeling agent such as fluorescence or enzymes. Over the last 30 years, SPR microscopy has become the major technique in label-free biodetection. The field of application range from the determination of affinity constant in biochemistry to the detection of pathogenic bacteria via cellular biology. Until now, the propagation length of the surface plasmons has been considered as the spatial resolution limit. However, many examples do not support this statement. In this PhD thesis, we demonstrate that the resolution is also limited by optical aberrations induced by the prism used to couple light and surface plasmons. Thus, we are able to explain why the experimental resolution was usually worse than the predicted one. The analysis of the image formation and the quantification of aberrations lead us to suggest two new optical configurations optimized for resolution. We also analyze which metal exhibits the better trade-off between propagation length and sensitivity. Experimentally, we obtain a resolution between 1.5 and 4 μm depending on the direction, on field-of-view up to several mm2, and with a standard sensitivity for biodetection (monolayer of DNA). We are then able to observe simultaneously several thousands of individual eukaryote and prokaryote cells. Finally, we develop a prototype dedicated to the real-time monitoring of protein secretion by immune cells. The limits of SPR microscopy and the solutions which could allow this kind of study are discussed. Preliminary results on the improvement of bacterial detection are also presented
Clavadetscher, Katarzyna Barbara. "Korrelation zwischen klinischen Risikofaktoren, radiologischen Veränderungen und Histologie von nicht-palpablen Befunden in der Mamma : eine Analyse von 183 lokalisierten Biopsien an der Universitäts-Frauenklinik Bern /." [S.l.] : [s.n.], 2002. http://www.stub.unibe.ch/html/haupt/datenbanken/diss/bestell.html.
Повний текст джерелаRobert, Michèle. "Etude quantitative des constituants épithéliaux et mésenchymateux dans l'hypertrophie bénigne de la prostate : comparaison des résultats obtenus sur pièces d'adénomectomie et biopsies uniques et multiples." Montpellier 1, 1995. http://www.theses.fr/1995MON1T038.
Повний текст джерелаSPAGNOLO, FRANCESCO. "Analisi fenotipica e funzionale dell’infiltrato linfocitario in biopsie di metastasi di melanoma, in pazienti in terapia con farmaci a bersaglio molecolare e/o inibitori dei checkpoint immunologici." Doctoral thesis, Università degli studi di Genova, 2020. http://hdl.handle.net/11567/1009806.
Повний текст джерелаTargeted therapies (TT) and immune checkpoint inhibitors immunotherapy (ICB) has dramatically changed the treatment of metastatic melanoma (MM). Although targeted therapy achieved a response rate as high as 70%, most patients ultimately develop resistance and progressive disease. Immune checkpoint inhibitors, especially with anti-PD-1 monoclonal antibodies, achieve durable responses, but in less than 40% of patients. The majority of patients receiving these treatments ultimately face progressive disease due to the development of primary of secondary resistance. Since only a fraction of patients achieve a durable benefit, the identification of predictive biomarkers is an unmet need. The objectives of our present study are: i) Identification of new molecular targets through the extensive in vitro and in vivo characterization of tumor biopsies; ii) Investigation of associations between PD-1/PD-L1/PD-L2/CTLA-4 variants and tumor microenvironment immunoscore with overall survival of patients receiving TT and ICB; iii) Analysis of the immune effects of TT and ICB on NK cells and their interaction with melanoma cells. Forty-eight patients with advanced melanoma were enrolled and 33 tumor biopsies from 29 patients were analyzed. Patients received TT and/or anti-PD-1 drugs. We observed that TIM3 expression is associated with PD-1 expression, as it is exclusively expressed in CD8+/PD-1 high T cells, while both GmzB and Eomes are also expressed by CD8+/PD-1 low cells. Moreover, CD8+/PD-1 high T cells show a higher Ki67 expression, suggesting that these cells may proliferate within the tumor. CD8+/PD-1 neg/low T cells are able to produce both IFN and TNF after polyclonal stimulation, while CD8+/PD-1 high cells produce low levels of TNF, maintaining the ability to release IFN. The association between PD-1/PD-L1 variants with immune infiltrate highlighted the role of PD1.5C>T and PD-L1C>T rs2297136 SNV in CD8+ cells recruiting. In particular, genotypes harboring the allelic variant T+ modify both the rate of CD8+ PD-1 high cells and the intensity of PD-1 high expression, compared with wild type genotypes.
von, Below Catrin. "PET and MRI of Prostate Cancer." Doctoral thesis, Uppsala universitet, Radiologi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-300940.
Повний текст джерелаNguyen, Thi Quynh Huong. "Insuffisance rénale chronique : épidémiologie de l'insuffisance rénale chronique chez l'enfant à l'Hôpital national pédiatrique de Hanoi et analyse histologique de l'expression du récepteur B1 de la bradykinine sur des biopsies de transplants rénaux." Toulouse 3, 2009. http://thesesups.ups-tlse.fr/918/.
Повний текст джерелаThe incidence of end stage renal disease (ESRD) has steadily increased in France and in all developed countries, representing a major public health problem. However, as in most developing countries, epidemiological data on the incidence of chronic kidney disease are greatly lacking in Vietnam, especially in children. The National Paediatric Hospital in Hanoi is a unique centre for treating chronic kidney diseases of all children from northern and central Vietnam. This work has revealed that the incidence of chronic renal failure in children is 5. 1 per million. Children are admitted to hospital very late and the rate of refusal of treatment is very high, especially in families not covered by social security (39% among beneficiaries against 72% among non-beneficiaries). The ESRD patients need replacement therapy, dialysis or transplantation, Kidney transplantation, when possible, is the best treatment for patients with ESRD. But despite the progress and steady improvement in the survival of kidney transplants, most of them develop long-term progressive deterioration of function due to the development and installation of chronic inflammation and interstitial fibrosis. The mechanism of the development of chronic kidney graft is a broad field of research in uro-nephrology. The bradykinin B1-receptor (B1R) is a pro-inflammatory receptor, hardly expressed in physiological conditions and induced in a large variety of tissues during chronic inflammation. The objective of this second part is to study the variation of the B1R expression during the first year following kidney graft by using an immunohistological analysis approach. This work shows that in these patients, the B1R expression is significantly correlated to the rate of creatinine (p <0. 05), the systolic blood pressure (p <0. 05) and finally to interstitial inflammation (p <0. 01). These encouraging results could lead to new therapeutic strategies in chronic allograft nephropathy. This thesis carried out in parallel to the National Paediatric Hospital in Hanoi, Vietnam and INSERM 858 unity, Toulouse, France, focuses on two major fields of kidney failure: epidemiology and physiological mechanisms
Obeid, Sameh. "Analyse quantitative et qualitative sur puce de vésicules extracellulaires en milieux complexes au sein d'une plateforme nanobioanalytique." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCD009/document.
Повний текст джерелаExtracellular vesicles (EVs) are small vesicles (30 to 1000 nm) released from different cell types, upon activation or apoptosis, and present in most body fluids (Blood, Urine….). Based on the current state of knowledge of their biogenesis and biochemical properties, EVs can be devided into three distinct populations: exosomes (EXO), microparticles (MPs) and apoptotic bodies (APOb). EVs have been found to play important biological roles and are also biomarkers of different pathologies. […] The first step consists of the injection of the samples containing EVs onto the biochip surface. This step is accomplished by SPR technique that allows label-free monitoring of EVs immunocapture onto the surface of a biochip presenting different specific bioreceptors. Following the capture of EVs, a nanometrological investigation of the biochip surface by AFM is engaged to characterize the physical properties of captured vesicles (size, morphology, etc..). Owning a nanometrical resolution, AFM can discriminate between individual EVs and vesicles or protein aggregates, leading to an accurate characterization of individual vesicles. The coupling of SPR technique with AFM was adapted to offer a representative global view of each array of bioreceptors and to measure the size of thousands of individual EVs. A proteomic investigation was also engaged to characterize the proteomic compositions of the different subpopulations of EVs. Such an investigation could contribute to the understanding of EVs biogenesis, biology and pathophysiology. To evaluate the potential of our platform to detect, quantify and characterize nanoparticles, two calibration particles, which cover the lower and upper size range of EVs, were chosen: (i) virus-like particles of 50 nm of diameter, also called CP50, and (ii) protein-functionnalized synthetic beads of 920 nm of diameter, called CP920. The capture tests in SPR showed a specific capture of these two calibration particles with their specific bioreceptors, immobilized onto the biochip surface, regardless the complexity of the media in which they were diluted. Also, a positive correlation was obtained between the capture level, measured by SPR, and the particle 9
Rimkevičius, Arvydas. "The Damage in Dermal Blood Vessels and Connective Tissue during Systemic Sclerosis (Histopathological and immunohistochemical biopsy analysis)." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100204_100416-45604.
Повний текст джерелаDisertacijos tema: Odos kraujagyslių ir jungiamojo audinio pažeidimai sergant sistemine skleroze. Disertacijos tikslas buvo nustatyti kraujagyslių pažeidimo ir fibrozės vystymosi eiliškumą, ištiriant kraujagyslių pakitimus odoje ir jungiamajame audinyje sergantiems sistemine skleroze , su šiai patologijai būdinga kraujagyslių atrezija, bei palyginant su kitomis būklėmis, kurių metu kraujagyslių atrezijos nėra (sistemine raudonaja vilklige), bei su būklėmis, kurioms būdingas kraujagyslių funkcinis nestabilumas (Reino sindromas). Tai retrospektyvus tyrimas, analizuota po 20 kiekvienos iš minėtų ligų sergančių ligonių ir kontrolinės grupės odos biopsijų. Be rutinių histocheminių dažymų naudata eilė imunohistocheminių žymenų ir elektroninės mikroskopijos tyrimas. Nustatyta, kad sergantiems sistemine skleroze odoje ankščiau, negu išreikšta fibrozė pasireiškia kraujagyslių endotelio pažeidimai ir padidėjęs kraujagyslių sienelės pralaidumas, kad kraujagyslių endotelio augimo veiksnio VEGF-A ir jo receptoriaus FLT-1, ir ypač HSP-47 (terminio šoko baltymo, kolagenams specifinio šaperono skatinančio fibrozę), bei eNOS (endotelio azoto sintazės) gausi ekspresija yra ankstyvos sisteminės sklerozės stadijos endotelio pakenkimo imunohistocheminiai žymenys. Nustatyta, kad ankstyvosios sisteminės sklerozės stadijos patogenezėje odoje vyrauja smulkių kraujagyslių pažeidimai ir į tą tikslinga atsižvelgti skiriant patogeneze pagristą sisteminės sklerozės gydymą Rekomenduota naudoti odos... [toliau žr. visą tekstą]
Kuhlmann, Jan Dominik [Verfasser], Sabine [Akademischer Betreuer] Kasimir-Bauer, Stephan [Akademischer Betreuer] Hahn, and Ralf [Akademischer Betreuer] Küppers. "Identifizierung neuer Biomarker für das Ovarialkarzinom – : Primärtumorbasierte Analysen und blutbasierte „Real-Time-Liquid-Biopsy“ / Jan Dominik Kuhlmann. Gutachter: Stephan Hahn ; Ralf Küppers. Betreuer: Sabine Kasimir-Bauer." Duisburg, 2013. http://d-nb.info/1035066416/34.
Повний текст джерелаBrychta, Nora [Verfasser], Matthias U. [Gutachter] Kassack, and Nikolas Hendrik [Gutachter] Stoecklein. "Analyse tumorspezifischer Mutationen in der Flüssigbiopsie (liquid biopsy) - Vergleich von zirkulierenden Tumorzellen und zirkulierender Tumor-DNA für die Frühdiagnose im Pankreaskarzinom / Nora Brychta ; Gutachter: Matthias U. Kassack, Nikolas Hendrik Stoecklein." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2017. http://d-nb.info/1149330546/34.
Повний текст джерелаZani, Emerson Luís 1975. "Profilaxia antibiótica na biópsia prostática transretal = revisão sistemática com metanálise." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308531.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: A biópsia prostática transretal (BPTR) é um procedimento bem estabelecido utilizado para a obtenção de tecido para o diagnóstico histológico do carcinoma da próstata. Apesar do fato de a BPTR ser geralmente considerada um procedimento seguro, ela pode ser acompanhada por complicações infecciosas e traumáticas. Embora as complicações infecciosas após a BPTR sejam bem conhecidas, há incerteza sobre a necessidade e a eficácia do antibiótico profilático de rotina e uma clara falta de padronização na profilaxia antibiótica para BPTR. O objetivo foi realizar uma revisão sistemática de ensaios clínicos randomizados sobre a profilaxia antibiótica em BPTR para avaliar a eficácia e os efeitos adversos do tratamento antibiótico profilático nessa situação. A pesquisa abrangeu as principais bases eletrônicas: MEDLINE, EMBASE, LILACS e Cochrane Central Register of Controlled Trials (CENTRAL). Especialistas foram consultados e referências de artigos relevantes foram obtidas. Todos os estudos randomizados e controlados (ERCs) de homens que se submeteram à BPTR e receberam antibióticos profiláticos ou placebo / nenhum tratamento foram selecionados, e também todos os ERCs avaliando um tipo de antibiótico contra outro, incluindo doses, vias de administração, freqüência de administração e duração do tratamento antibiótico. A revisão sistemática foi conduzida na Colaboração Cochrane. No geral, mais de 3500 referências foram analisadas e dezenove artigos originais com um total de 3599 pacientes foram incluídos. Nove estudos analisaram antibióticos versus placebo / nenhum tratamento, com todos os resultados favorecendo significativamente o uso de antibióticos (P <0,05 (I2=0%)), incluindo bacteriúria (risco relativo (RR) 0,25 (intervalo de confiança (IC) de 95% de 0,15 a 0,42), bacteremia (RR 0,67, IC 95% 0,49-0,92), febre (RR 0,39, IC 95% 0,23-0,64), infecção do trato urinário (RR 0,37 (IC 95% 0,22-0,62) e hospitalização (RR 0,13 (IC 95% 0,03-0,55)). Diversas classes de antibióticos foram efetivas profilaticamente para a BPTR, e a classe das quinolonas foi a melhor classe analisada, com o maior número de estudos (cinco) e de pacientes (1188). Na comparação "antibiótico versus enema", foram analisados quatro estudos com um número limitado de pacientes. As diferenças entre os grupos não foram significativas. Para "antibiótico versus antibiótico + enema", apenas o risco de bacteremia (RR 0,25, IC 95% 0,08-0,75, P = 0,01) foi reduzido no grupo "antibiótico + enema". Sete estudos relataram os efeitos de antibiótico de curta duração (um dia) versus curso de longa duração (três dias). O uso de antibióticos por longo curso foi significativamente melhor do que o tratamento de curta duração apenas para bacteriúria (RR 2,09, IC 95% 1,17-3,73, P = 0,01 (I2=34%)). Para "dose única versus múltiplas doses", houve maior risco de bacteriúria com dose única (RR 1,98, IC 95% 1,18-3,33, P <0,05 (I2%=7)). Comparando-se a administração oral versus sistêmica - injeção intramuscular (IM) ou intravenosa (IV) - dos antibióticos, não houve diferenças significativas entre os grupos para bacteriúria, febre, ITU e hospitalização. A profilaxia antibiótica é eficaz na prevenção de complicações infecciosas após BPTR. Diversas classes de antibióticos são eficazes profilaticamente para a biópsia da próstata e a classe das quinolonas foi a classe melhor analisada, com o maior número de estudos e de pacientes. Não há dados definitivos para confirmar que os cursos antibióticos de longa duração (três dias) sejam superiores aos tratamentos de curta duração (um dia), ou que o tratamento com doses múltiplas seja superior ao de uma dose única
Abstract: Transrectal prostate biopsy (TRPB) is a well established procedure used to obtain tissue for the histological diagnosis of carcinoma of the prostate. Despite the fact that TRPB is generally considered a safe procedure, it may be accompanied by traumatic and infective complications. Although infective complications after TRPB are well known, there is uncertainty about the necessity and effectiveness of routine prophylactic antibiotics and a clear lack of standardization in antibiotic prophylaxis for TRPB. The objective was to conduct a systematic review of randomized controlled trials on antibiotic prophylaxis in TRPB to evaluate the effectiveness and adverse effects of prophylactic antibiotic treatment in this situation. The search covered the principal electronic databases: MEDLINE, EMBASE, LILACS and the Cochrane Central Register of Controlled Trials (CENTRAL). Experts were consulted and references from the relevant articles were scanned. All randomized, controlled trials (RCTs) of men who underwent TRPB and received prophylactic antibiotics or placebo/no treatment were selected, and all RCTs looking at one type of antibiotic versus another, including comparable dosages, routes of administration, frequency of administration, and duration of antibiotic treatment. The systematic review was conducted in Cochrane Collaboration. Overall, more than 3500 references were considered and nineteen original reports with a total of 3599 patients were included. There were nine trials analyzing antibiotics versus placebo/no treatment, with all outcomes significantly favoring antibiotic use (P < 0.05 (I2 = 0%)), including bacteriuria (relative risk (RR) 0.25 (95% confidence interval (CI) 0.15 to 0.42), bacteremia (RR 0.67, 95% CI 0.49 to 0.92), fever (RR 0.39, 95% CI 0.23 to 0.64), urinary tract infection (RR 0.37 (95% CI 0.22 to 0.62), and hospitalization (RR 0.13 (95% CI 0.03 to 0.55)). Several classes of antibiotics were effective prophylactically for TRPB, and the quinolones were the best analyzed class, with a higher number of studies (five) and patients (1188). For 'antibiotic versus enema', we analyzed four studies with a limited number of patients. The differences between groups were not significant. For "antibiotic versus antibiotic + enema", only the risk of bacteremia (RR 0.25, 95% CI 0.08 to 0.75, P = 0.01) was diminished in the "antibiotic + enema" group. Seven trials reported the effects of short-course (1 day) versus long-course (3 days) antibiotics. Long course was significantly better than short-course treatment only for bacteriuria (RR 2.09, 95% CI 1.17 to 3.73, P = 0.01 ( I2 = 34%)). For "single versus multiple dose", there was significantly greater risk of bacteriuria for singe-dose treatment (RR 1.98, 95% CI 1.18 to 3.33, P < 0.05 (I2 = 7%)). Comparing oral versus systemic administration - intramuscular injection (IM), or intravenous (IV) - of antibiotics, there were no significant differences in the groups for bacteriuria, fever, UTI and hospitalization. Antibiotic prophylaxis is effective in preventing infectious complications following TRPB. Several classes of antibiotics are effective prophylactically for prostate biopsy and the quinolones were the best analyzed class, with a higher number of studies and patients. There is no definitive data to confirm that antibiotics for long-course (three days) are superior to short-course treatments (one day), or that multiple-dose treatment is superior to single-dose
Doutorado
Fisiopatologia Cirúrgica
Doutor em Ciências
Corrêa, Maria Elvira Pizzigatti. "A mucosa oral e glandulas salivares acessorias na doença do enxerto contra o hospedeiro cronica pos transplante de medula ossea : estudo prospectivo dos primeiros 63 transplantes de medula ossea : analise comparativa com parametros clinicos e biopsia de pele Hemocentro/Unicamp." [s.n.], 1999. http://repositorio.unicamp.br/jspui/handle/REPOSIP/311043.
Повний текст джерелаDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: A Doença do Enxerto contra o Hospedeiro representa uns dos fatores de maior morbidade e mortalidade pós transplante alogênico de medula óssea (TMO). Lesões na cavidade bucal e em glândulas salivares menores são achados comuns na GVHDc, manifestando-se através de xerostomia e lesões liquenóides. O objetivo deste trabalho foi estudar, prospectivamente, os aspectos clínicos e histopatológicos da mucosa bucal e glândulas salivares acessórias no dia +100 (coletados necessariamente, conforme protocolo) e no momento do diagnóstico da GVHDc feitao posteriormente ao dia 100 pós TMO, para: avaliar a especificidade e sensibilidade da análise histopatológica protocolar do dia +100; estudar a freqüência de acomentimento da pele, mucosa bucal e glândula salivar na GVHD crônica; determinar os parfun.etros clínicos associados à sobrevida pós transplante; determinar os parâmetros clínicos preditivos de GVHD crônica e detenninar o valor da análise da mucosa bucal e glândula salivar acessória na sobrevida pós transplante de medula óssea. Foram coletadas ao redor do dia +100, amostras de pele e mucosa bucal incluindo glândula salivar menor de 45 pacientes submetidos ao TMO alogênico HLA-idêntico. Uma segunda biópsia foi realizada em 25 pacientes que desenvolveram ou mantiveram sintomas sugestivos de GVHDc. As amostras foram analisadas e classificadas de acordo com o proposto por HORN (1995). Os resultados foram analisados estatisticamente pelo modelo tmivariado da regressão de azares proporcionais de COX Para avaliar as variáveis significativas em conjunto, foi utilizado o modelo multivariado de regressão de azares proporcionais de COX (regressão stepwise). Para análise de sobrevida foram construídas curvas de Kaplan-Meir. Do total de 63 transplantes realizados no Serviço de Transplante de Medula Óssea do Hemocentro da Unicamp entre setembro de 1993 a maio de 199~, 45 pacientes entraram no estudo, por sobreviverem mais que 100 dias pós transplante. Todos os pacientes receberam transplante alogênico, HLA-idêntico. Desses 45 pacientes, 32 receberam enxertos coletados por aspiração da medula óssea (MO) e 13 por células progenitoras periféricas (CPP). Do grupo, 19 pacientes eram mulheres 26 homens, com idade mediana de 36 anos (4 - 57). Os resultados do exame histológico no dia 100 não demonstraram valores preditivos para o diagnóstico para o GVHDc. O tipo de transplante realizâdo, CPP (p=0,023), a presença anterior da GVHD aguda (p=O,OO16) e doadoras femininas para receptores masculinos (p=O,031) demonstraram ser fatores preditivos para a GVHD crônica. Em relação à sobrevida, o tipo de transplante, CPP (p=0,017), a antecedência da forma aguda da GVHD (p=0016) e o nível sérico maior de ciclosporina (p=O,0031) foram correlacionados a um pior prognóstico. A segunda biópsia foi realizada num intervalo entre 104 a 304 dias pós TMO (mediana de 195 dias). Dezoito pacientes apresentaram GVHDc extensa, 4 localizada e 3 não apresentaram GVHDc. Onze pacientes morreram depois da segunda avaliação, sendo que 7 por GVHDc, 3 por recaída da leucemia e um por causa não relativa ao TMO. Nesse período, os achados histológicos positivos para GVHDc em mucosa bucal e glândula salivar menor foram estatisticamente significativos em relação à sobrevida dos pacientes. Os achados foram classificados em 3 grupos de gravidade da GVHDc, de acordo com a classificação de Hom (Grau I, 11, III-IV). A mucosa bucal (p=O,02) e a glândula salivar menor (p=O,OO9) demonstraram ter valor preditivo para a sobrevida dos pacientes. Apesar do pequeno número de pacientes, nossos dados sugerem que as biópsias protocolares no dia + 1 00 não são adequadas ao diagnóstico de GVHDc. A glândula salivar revela com maior freqüência a agressão linfocitária da GVHDc que a pele ou mesmo a mucosa bucal. A presença da fase aguda da GVHD, assim como doadoras femininas e o tipo de transplante realizado demonstraram ser fatores importantes no prognóstico menor de sobrevida. A GVHD aguda, assim como o tipo de transplante (CPP), e o sexo do doador (feminino) demonstraram ser importantes fatores preditivos de desenvolvimento da GVHD crônica. Os achados histológicos positivos para GVHDc em glândula salivar juntamente com os de mucosa bucal podem refletir em pior prognóstico (sobrevida)
Abstract: Chronic graft-vs-host disease (cGVHD) is an important cause of morbity and mortality after allogeneic bone marrow transplants. Lesions ofthe oral mucosa and salivary glands are common features of cGVHD, manifesting as xerostomia, lichenoid lesions or even severe mucositis. The biopsy of specimens of the oral mucosa, collected on day +100, is commonly used as a screening test for the diagnosis af cGVHD. The aim of our study was to evaluate the screening test for diagnosis of cGVHD on day +100 post BMT, the predictive factors of diagnosis for cGVHD, the incidence of skin and oral cGVHD manifestation and the use of findings of the histologycal specimens as a predictor of shorter survival in patients with cGVHD. Data were initially analyzed from 45 patients who had received unrnodified allogeneic marrow grafts in the Bone Marrow Transplantation Hospital Service / Hematology and Hemotherapy Center of the State University ofCampinas, UNICAMP, between September 1993 to May 1995 and who had survived for over 100 days. Ofthese 45 patients, 32 received grafts collected byaspiration (bone marrow - BM) and 13 peripheric bone progenitors cells (pBPC), from HLA-identical siblings. Biopsies of the skin and labial mucosa, including representative samples of minor salivary gland (MSG), were collected on day +100. A second biopsy was performed at a later time in 25 patients (BM=16, PBPC=9) who had developed or sustained signs or symptoms suggestive of cGVHD. All specimens were analised by 2 examiners (MEPC and MLC) separately for later comparison. Hom pathological staging score was used for the histological classification of the oral and salivary gland findings. Univariate and multivariate Cox proportional hazard regression models, and the Kaplan-Meir method were used to determine the overall survival. Sixty-three allogeneic BMT wesre performe~ between September 1993 and May 1995 in the Bone Marrow Service of the Hemocentro / Unicamp. Forty-five patients suvived for over 100 days and the histological findings were analised. Df these patients, 32 received bone marrow transplantation (BMT) and 13 received peripheral bane progenitor cells (PBPC). Nineteen patients were women and 26 men and the median age was 36 years (4 - 57 years old). The histological findings, obtained at the +100 day did not show any predictive value for cGVHD diagnosis. PBPC (p=0,023) transplant, acute GVHD,.sphO,0016) and female donors (p=0,031) were predictive factors for cGVHD on the 100 day post BMT. The PBPC (p=O,Ol7) transplant, acute GVHD (p=O,0016) and the leveI of cysclosporin (p=0,0031) were predictive factors for a worst prognostic of overall survival. The second time biopsies were performed from 104 to 304 days after BMT (median 195 days). Eighteen patients showed extensive cGVHD, 4 localized cGVHD and 3 no formof cGVHD. Eleven patients died after the second biopsy, 7 due to cGVHD, 3 due to leukemia relapse and one due to no transplant reason. The skin histological fmdings did not show any prognostic value. The results of histologicaloral mucosa and minor salivary gland fmdings showed statistical importance. We stratified patients into three groups of scale cGVHD severity, using the HORN pathological staging score (1, 11 and III-IV). For oral mucosa, the statistical value was p=0,02 for and the minor salivary gland it was p=0,009. Despite the small number of patients, our results suggest that severe histologic lesions in MSG specimens obtained beyond day +100 correlate with higher mortality in patients suffering from cGVHD. There is a greater frequency of lymphocyte infiltration in minor salivary gland than in the oral mucosa or skin. The histological findings on the 100 dayafter BMT, did not show any prognostic value for diagnosis of cGVHD. The female danors and the type oftransplantation showed important value in the prognostic for overall survival for BMT patients. The aGVHD, the BMT type and the sex of the donor were important factors for prognostic of cGVHD
Mestrado
Anatomia Patologica
Mestre em Ciências Médicas
Barbosa, Hugo Perazzo Pedroso. "O diagnóstico da cirrose hepática: comparação da laparoscopia com a histologia." Universidade do Estado do Rio de Janeiro, 2009. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=2157.
Повний текст джерелаThe diagnosis of hepatic cirrhosis is very important in the management of patients with chronic liver diseases. Retrospective series reported percutaneous liver biopsy to miss cirrhosis in about 30%. The aim of this study was to compare the accuracy of liver descriptions made during laparoscopy with liver histology found by laparoscopic biopsy in patients with chronic hepatitis. We also described the complications rates of the laparoscopic liver biopsy, estimated the influence of the length of liver fragment and the Kappas índice in the diagnosis of cirrhosis. Consecutive patients were prospectively submitted to laparoscopic liver biopsy (14G needle) by two independent investigators blind to clinical, laboratorial and ultra-sonographic findings. Liver specimens were assessed blindly according to the modified Ishak score. The sensitivity, specificity, accuracy, positive and negative predictive values were evaluated for the laparoscopy and histology in the diagnosis of cirrhosis. A p-value of 0.05 was considered statistically significant. Eighty-four patients were included, 55% male sex, 85% with chronic HCV infection, median age 47 11 years. The median length of the biopsy sample and the numbers of portal tracts was 2.9 1,0 cm and 12 5, respectively. The histological sensitivity (89% x 71%), negative predictive value (92% x 83%) and accuracy (95% x 88%) were better in comparison with laparoscopy in cirrhosis diagnosis. The Kappas indices for cirrhosis diagnosis between the two investigators was 0.80. Cirrhotic patients had more fragmented (p= 0,048) and bigger samples (p=0.05) than non-cirrhotics. Patients with mild disease in microscopic analysis (F ≤ 2) had less numbers of portal tracts (8 4 x 13 4 p<0.001) and smaller samples size (p=0.036) than those with moderate/severe disease. There were no earlier complications related to the laparoscopy and 9.6% of late complications, all of that associated with a endemic outbreak of atypical micobacteriosis that happened in Rio de Janeiro during this study. There were 6% of hepatic biopsys minor complications, all bleeding at the biopsy site controlled during the laparoscopy. There were no major complications. There was 5% of gain made by liver laparoscopic evaluation in the cirrhosis diagnosis. The length of the hepatic fragment, however, had great influence in the diagnosis of cirrhosis. Probably our big sample size (2,9 1,0 cm) surpass the problem of understaging of the liver biopsies. The Kappas indices between the investigators were excellent. The laparoscopic liver biopsy is a safe procedure.
Ronkainen, J. (Johanna). "Costs in today's radiology:aBC analysis of typical situations in the transitional period." Doctoral thesis, University of Oulu, 2007. http://urn.fi/urn:isbn:9789514284816.
Повний текст джерелаOffranc, Piret Gaëlle. "Nanofils de silicium pour analyse sensible de biomolécules par spectrométrie de masse et pour l'adressage fluidique de cellules en vue des applications laboratoires sur puce et biopuces." Phd thesis, Université des Sciences et Technologie de Lille - Lille I, 2010. http://tel.archives-ouvertes.fr/tel-00491178.
Повний текст джерелаRimkevičius, Arvydas. "Odos kraujagyslių ir jungiamojo audinio pažeidimai sergant sistemine skleroze (Histopatologiniai ir imunohistocheminiai bioptatų tyrimai)." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100204_100318-37216.
Повний текст джерелаDissertation topic: the damage to dermal blood vessels and connective tissue during systemic sclerosis. The aim of this dissertation was to determine the sequence in the development of vascular damage and fibrosis by studying vascular changes in the skin and connective tissue of systemic sclerosis patients with vascular atresia characteristic of this pathology and by comparing it with other conditions, where there is no vascular atresia (systemic lupus erythematosus), and with conditions, which are characterised by vascular function instability (Raynaud’s phenomenon). This was a retrospective study, which analysed 60 patients with the aforementioned diseases, 20 with each disease, and a control group of 20 skin biopsies. In addition to routine histochemical stains, a series of immunohistochemical signs and electron microscopic examination were used. It was determined that damage to the vascular endothelium and increased permeability of the vascular walls appear earlier than expressed fibrosis in the skin of systemic sclerosis patients and that the abundant expression of vascular endothelial growth factor (VEGF)-A and its receptor FLT-1 and especially HSP-47 (heat shock protein, a collagen-specific chaperone that induces fibrosis) and eNOS endothelial (nitric oxide synthase) are immunohistochemical signs of endothelial injury in the early stage of systemic sclerosis. It was determined that in the pathogenesis of the early stage of systemic sclerosis, damage to the small blood... [to full text]
Choi, JungHun. "Design and Development of a Minimally Invasive Endoscope: Highly Flexible Stem with Large Deflection and Stiffenable Exoskeleton Structure." Diss., Virginia Tech, 2006. http://hdl.handle.net/10919/26218.
Повний текст джерелаPh. D.
ARAUJO, RENATO M. "Caracterização clínica e epidemiológica da neoplasia prostática nos anos de 2012 a 2014 em um Centro de Oncologia do leste de Minas Gerais." reponame:Repositório Institucional do IPEN, 2017. http://repositorio.ipen.br:8080/xmlui/handle/123456789/28020.
Повний текст джерелаMade available in DSpace on 2017-11-17T17:09:23Z (GMT). No. of bitstreams: 0
O câncer de próstata (CaP) é a segunda causa mais comum de câncer em homens. De acordo com o INCA, no Brasil, em 2016, estimam-se aproximadamente 61.200 novos casos de câncer de próstata. Objetivo: Identificar as características demográficas e epidemiológicas, bem como dados do estadiamento tumoral dos pacientes com CaP atendidos na Unidade de Oncologia do Hospital Marcio Cunha na cidade de Ipatinga-MG nos anos de 2012, 2013 e 2014. Metodologia: Trata-se de um estudo retrospectivo e descritivo onde foram analisados 668 prontuários de pacientes, com registro do diagnóstico anatomopatológico, atendidos nos anos de 2012, 2013 e 2014, conforme lista fornecida pela instituição, com diagnóstico de CaP cadastrados com CID-10 - C 61. As variáveis analisadas foram: procedência, ano do diagnóstico, faixa etária, raça autodeclarada, fatores de risco como tabagismo, etilismo, história familiar de CaP, PSA total ao diagnóstico, tipo histológico da biópsia, score de Gleason da biópsia, tipo histológico da peça cirúrgica, score de Gleason da peça cirúrgica. Os dados foram analisados empregando-se estatística descritiva e inferencial, utilizando o software SPSS, versão 19.0. Resultados: A maior incidência de casos de CaP foram provenientes das cidades mais populosas da microrregião de saúde analisada e faixa etária mais prevalente foi entre 61 e 80 anos com prevalência em pardos e brancos e com histórico familiar de 17,2% de parentes de primeiro grau; com o pai em 37,3%, o irmão em 60,8% e filho em 1,9%. Apenas 165 (25,9 %) eram fumantes e 20,8% etilistas. Os níveis de PSA ficaram entre 4,1ng/ e 10ng/ml (49,5%) e quanto maior a faixa etária maiores os valores do PSA. Pacientes pardos apresentaram PSA total mais elevado. Ao avaliarmos se existia relação entre os níveis de PSA total com fatores de risco como tabagismo, etilismo e histórico familiar, somente houve relação estatisticamente significativa com o etilismo. Houve concordância do score de Gleason entre biópsia e peça cirúrgica em 70%, subgraduação em 18,7% e supergraduação em 11,3%. Comparando a idade dos pacientes com Score de Gleason, quanto maior a idade do paciente maior foi o Score de Gleason do material obtido pela biópsia via transretal Pacientes tabagistas e etilistas apresentaram Score de Gleason da peça cirúrgica mais elevados. Conclusão: A concordância entre o Score de Gleason da biópsia e o Score de Gleason da peça cirúrgica foi de 70%; etilistas apresentaram PSA mais elevados; quanto maior foi a faixa etária, mais indiferenciado foi o tumor ( biópsia); pacientes tabagistas e etilistas apresentaram tumores mais indiferenciados na peça cirúrgica; este é o primeiro estudo epidemiológico de CaP desenvolvido na região do Vale do Aço, a caracterização sócio demográfica e as associações aqui encontradas podem contribuir com programas para desenvolver ações de controle do CaP nesta região.
Dissertação (Mestrado em Tecnologia Nuclear)
IPEN/D
Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP
Fahr, Florian [Verfasser], Franz Maximilian [Akademischer Betreuer] Rasche, Joachim [Akademischer Betreuer] Beige, Christian [Gutachter] Wittekind, and Matthias [Gutachter] Girndt. "Zeitliche und räumliche Analyse histomorphologischer Befunde aus Eigennierenbiopsien im Raum Leipzig über einen Zeitraum von 20 Jahren : Temporal and spatial analysis of renal biopsy data collected in the metropolitan area of Leipzig during a time frame of 20 years / Florian Fahr ; Gutachter: Christian Wittekind, Matthias Girndt ; Franz Maximilian Rasche, Joachim Beige." Leipzig : Universitätsbibliothek Leipzig, 2016. http://d-nb.info/1240696124/34.
Повний текст джерелаFRANCONE, ELISA. "MiRNAs’ characterization in metastatic colorectal cancer: a multicenter prospective translational study." Doctoral thesis, Università degli studi di Genova, 2020. http://hdl.handle.net/11567/1002660.
Повний текст джерелаKollander, Barbro. "Inductively Coupled Plasma Atomic Emission Spectrometry : Exploring the Limits of Different Sample Preparation Strategies." Doctoral thesis, Uppsala universitet, Analytisk kemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-150861.
Повний текст джерелаGerin, Chloé. "Modélisation et étude histologique de gliomes diffus de bas grade." Phd thesis, Université Paris-Diderot - Paris VII, 2012. http://tel.archives-ouvertes.fr/tel-00820353.
Повний текст джерелаNunes, Vera Lopes. "O papel do acúmulo do colágeno miocárdico intersticial na sobrevida dos pacientes com miocardiopatia dilatada idiopática e chagásica." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/5/5131/tde-19032007-160751/.
Повний текст джерелаDilated cardiomyopathies represent 87% of all cardiomyopathies and they have adverse prognosis with high morbidity and mortality. There are several prognostic markers, however, a structural one has not been described yet. Seems to be very important to find out whether morphological changes upon myocardial structure would affect the prognosis. We studied, using endomyocardial biopsy and 2D-echocardiogram, 9 patients with no structural myocardial changes (control) and 45 patients with severe dilated cardiomyopathies. They were divided according the etiology of cardiomyopathy into idiopathic group (IDCM) or Chagas group (CDCM). We analyzed the correlation between interstitial myocardial collagen (ICVF) and survival rates. We also evaluated the difference of ICVF between these groups and whether it correlates with geometric and functional changes of the heart. We observed that ICVF was 15 times higher in cardiomyopathies patients than in control group, but it did not differ between CDCM and IDCM (ICVF% CDCM = 6.83 ± 5.47; IDCM = 5.75 ± 4.45; control = 0.42 ± 0.14*; p<0.001). The ICVF did not correlate to survival rate in cardiomyopathies patients (IDCM-ICVF £5.53 (20.0%) or >5.53 (0.0%) (p=0.249), and CDCM-ICVF £5.53 (0.0%) or >5.53 (7.7%) (p=0.587). We observed a significant correlation between ICVF and left ventricular ejection fraction (LVEF) only on DMC, the ICVF did not correlate to left ventricular diastolic diameter in either etiology. Conclusion: the myocardial fibrosis did not differ between these two etiologies, it did not correlate to prognosis either in the IDCM or CDCM and only in the IDCM the ICVF correlated to the LVEF.
Cowan, Nigel Christopher. "The development of CT urography for investigating haematuria." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:284084de-2a71-4e35-8342-41f039b03df1.
Повний текст джерелаSchilling, Anne. "Die Endometriumbiopsie bei der Stute- eine Analyse der histologischen Befunde zwischen 1992- 2012 am Leipziger Institut für Veterinär- Pathologie." Doctoral thesis, 2016. https://ul.qucosa.de/id/qucosa%3A15630.
Повний текст джерелаTabet, Paul. "Masses kystiques latérales du cou : une analyse comparative des approches diagnostiques." Thèse, 2019. http://hdl.handle.net/1866/23670.
Повний текст джерелаBenign and malignant lateral cystic neck masses (LCNM) are difficult to distinguish clinically. The usefulness of imaging and sampling modalities in clarifying the diagnosis remains unclear. Retrospective review of cases between 2010 and 2016. Imaging data was reviewed and the variables pertaining to the mass were assessed including the following: size, nodal level, fat stranding, extracapsular spread, calcifications, vascularity, necrosis and standardized uptake value. Sampling reports of fine-needle aspiration (FNA), core-needle biopsy (CNB) and frozen section (FS) were also assessed. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for predicting malignancy were calculated for all variables and compared between benign and malignant cystic neck masses. Ultrasound was used in 47.2% and CT-Scan in 90.5% of patients. No variables on imaging could definitely differentiate benign from malignant LCNM. FNA had a lower sensitivity then CNB (59% vs 83%; p=0.036) and FS (59% vs 93%; p=0.01). FS had a better NPV when compared to FNA (92% vs 40%; p<0.001) and CNB (92% vs 50%; p=0.062). Specificities and PPV were similar among all groups. Clinicians cannot rely on imaging to differentiate benign from malignant LCNM. Given its adequate PPV (92%), FNA should be used initially on lateral cystic neck masses. Because of its high sensitivity, CNB should be considered if FNA is not diagnostic of malignancy. FS should always follow a CNB not indicative of malignancy, because of the low NPV. Any result diagnostic of malignancy on either FNA, CNB or FS strongly indicates presence of malignancy.
Bratu, Vlad Antonio [Verfasser]. "Histopathological morphometry of human endobronchial biopsies : a comparison of conventional quantitative analyses and stereological designs / vorgelegt von Vlad Antonio Bratu." 2008. http://d-nb.info/999867547/34.
Повний текст джерела"Novel methods for specific detection and quantification of covalently closed circular DNA in sera and biopsies of hepatitis B patients." Thesis, 2011. http://library.cuhk.edu.hk/record=b6075203.
Повний текст джерелаOver 350 million people worldwide suffer from chronic hepatitis B virus (HBV) infection, which leads to many cases of cirrhosis and hepatocellular carcinoma. HBV covalently closed circular DNA (cccDNA) is a critical intracellular replicative intermediate and cannot be eliminated during antiviral therapy. Current methods for cccDNA detection are limited by false positive detection due to the interference by HBV relaxed circular DNA (rcDNA). The tests also have limited sensitivity to detect cccDNA at low concentrations. Hence, we aimed to develop a highly sensitive and highly specific assay for cccDNA detection with wide linear range.
The modified Bowden's assay had the highest intrahepatic cccDNA detection rate (60 positive results out of 61 cases). The detection rate of the modified Bowden's assay is significantly higher than that of the Bowden's assay. On the other hand, the cccDNA detection rate in serum samples was low at 20--27% by all 3 assays. In 5 samples in which cccDNA was undetectable by the Bowden's assay but detectable by the other two assays, a point mutation in the HBV genome was found in the forward primer binding site of the Bowden's assay. This partly explained the false negative results.
The quantification result of cccDNA by the bisulfite conversion assay was significantly lower than that by the Bowden's assay assay (P=0.001) and the modified Bowden's assay (P=0.003). When the total HBV DNA was higher than 107 copies/ml, the serum cccDNA level detected by the bisulfite conversion assay was significantly lower than that detected by the Bowden's assay (P=0.008) and the modified Bowden's assay (P=0.046). When the total HBV DNA is less than 107 copies/ml, there were no significant differences. This suggests that the bisulfite conversion assay was less affected by rcDNA even in samples containing a high viral load.
With this background, two new cccDNA assays were developed and optimized. Bowden's assay was used as a standard to evaluate the performance of new assays. The first new assay (modified Bowden's assay) involved the use of new primers and probes that targeted more conserved regions in the HBV genome. The second assay adopted the bisulfite conversion method, which introduced gene sequence changes into the HBV genome and thereby enhance the specificity of the assay. Capillary sequencing was performed to find mutations in primers and probe range of different assays.
Yu, Ling.
Advisers: Vincent Wai-Sun Wang; Joseph Jao-Yiu Sung.
Source: Dissertation Abstracts International, Volume: 73-06, Section: B, page: .
Thesis (Ph.D.)--Chinese University of Hong Kong, 2011.
Includes bibliographical references (leaves 105-111).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Abstract also in Chinese.
Conradi, Lena-Christin [Verfasser]. "Prädiktives und prognostisches Potential der Thymidylatsynthase als Biomarker im multimodalen Therapiekonzept 5-FU-basierter Radiochemotherapie des lokal fortgeschrittenen Rektumkarzinoms : immunhistochemische Analyse prätherapeutischer Biopsien und korrespondierenden residuellen Tumorgewebes / vorgelegt von Lena-Christin Conradi." 2010. http://d-nb.info/1008992038/34.
Повний текст джерелаČuperková, Romana. "Optimalizace postupů pro kvantifikaci miRNA z tenkojehlových bioptických vzorků karcinomu pankreatu." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-337129.
Повний текст джерелаWells, Bryan John. "A Comparison of the Costs of Sentinel Lymph Node Biopsy and of Axillary lymph Node Dissection in the Management of Early-stage Breast Cancer in Ontario." Thesis, 2009. http://hdl.handle.net/1807/18993.
Повний текст джерелаLemos, Mariana Timóteo. "Biópsia de gânglio sentinela em melanomas espessos: estudo clínico retrospetivo." Master's thesis, 2015. http://hdl.handle.net/10316/30572.
Повний текст джерелаIntrodução: A biópsia de gânglio sentinela (BGS) é uma técnica utilizada rotineiramente no estadiamento de doentes com melanoma cutâneo, estando o seu valor prognóstico e terapêutico bem definido nos melanomas de espessura intermédia (1-4 mm). Contudo, nos melanomas espessos (>4 mm) existe ainda alguma controvérsia quanto à sua utilidade. Objetivo: Avaliar o valor prognóstico do status do gânglio sentinela em doentes com melanomas espessos. Material e Métodos: Utilizando curvas de Kaplan-Meier e o modelo de regressão de Cox calcularam-se o tempo livre de doença (TLD) e o tempo de sobrevivência global (TSG) numa amostra de doentes com diagnóstico de melanoma espesso. O efeito de variáveis como idade, sexo, espessura do melanoma, presença de ulceração, tipo e localização do melanoma, no TSG e no TLD destes doentes também foi avaliado. Resultados: Estudaram-se 43 doentes com melanoma espesso (21 do sexo feminino e 22 do sexo masculino), com uma média etária de 63,88 anos. A BGS revelou-se positiva em 20 doentes (46.5%) e negativa em 23 (53.5%). O tempo médio de seguimento foi de 40 meses. Um total de 15 doentes (35%) morreram em relação com a progressão do melanoma e 22 doentes (51%) sofreram recidiva, não havendo diferença estatisticamente significativa entre o grupo de BGS positiva e o grupo de BGS negativa. A TSG foi tendencialmente menor nos doentes com BGS positiva (taxa de sobrevivência aos 5 anos de 52% versus 79% no grupo de BGS negativa). No entanto, esta tendência não atingiu significado estatístico. Os doentes com BGS negativa apresentaram um maior TLD, em comparação com os doentes com BGS positiva (taxa de sobrevivência livre de doença aos 5 anos de 63% versus 19%, respetivamente, com significado estatístico). A única variável que demonstrou influenciar o TSG e o TLD foi a espessura do melanoma. Conclusão: Este trabalho demonstrou que a BGS não prediz o tempo de sobrevivência global em melanomas espessos, provavelmente devido ao elevado risco de disseminação hematogénea. Contudo, o estado do gânglio sentinela contribui com importante informação prognóstica, uma vez que é um importante preditor do tempo livre de doença. Por este motivo, a biópsia do gânglio sentinela deve ser recomendada a todos os doentes com melanoma espesso.
Background: Sentinel-node biopsy (SNB) is a widely accepted technique in the staging and management of patients with malignant melanoma. Its prognostic value is well established in intermediate-thickness melanomas (1-4 mm). However, its use in the management of patients with thick melanoma (> 4 mm) is still controversial. Objective: To assess the prognostic value of sentinel node status in patients with thick melanoma. Patients and Methods: The disease free survival (DFS) and the overall survival (OS) were estimated using Kaplan-Meier curves and Cox regression model in a sample of patients with thick melanoma. The influence of other parameters such as patient's age and sex, melanoma thickness, ulceration, type and location over the DFS and OS were also assessed. Results: Twenty-one (48.8%) out of 43 patients with thick melanoma were female and 22 (51.2%) were male, with a mean age of 63.88 years. SNB was positive in 20 (46.5%) patients and negative in 23 (53.5%) patients. Mean follow-up time was 40 months. Overall, 15 (35%) patients suffered a melanoma-related death, and 22 (51%) patients had a disease relapse, with no significant differences between patients with positive BGS and patients with negative BGS. The 5 year overall survival rate was tendentiously lower (52%) in patients with positive BGS than in patients with negative BGS (79%), but lacking statistic significance. Patients without sentinel node metastases had a 5-year disease survival rate significantly higher 63% than those with positive SNB (19%). Despite the sentinel node status, the only parameter which showed significant influence in OS and DFS was tumor thickness. Conclusion: This study showed SNB does not predict overall survival in patients with thick melanoma, probably due to the high risk of hematogenous spread. However, sentinel-node biopsy provides important prognostic information, since it predicts disease free survival in patients with thick melanomas, and therefore it should be recommended to all patients with thick melanoma.