Дисертації з теми "Biomolecular Sensors"
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Marti, Villalba Maria. "Biomolecular engineered sensors for diagnostic applications." Thesis, Nottingham Trent University, 2009. http://irep.ntu.ac.uk/id/eprint/363/.
Повний текст джерелаCooper, Emily Barbara 1977. "Silicon field-effect sensors for biomolecular assays." Thesis, Massachusetts Institute of Technology, 2003. http://hdl.handle.net/1721.1/87450.
Повний текст джерелаIncludes bibliographical references.
System-level understanding of biological processes requires the development of novel biosensors capable of quantitative, real-time readout of molecular interactions. Label-free detection methods can minimize costs in time and resources by obviating preparatory steps necessary with label-based methods. They may further be valuable for monitoring biomolecular systems which are difficult or impossible to tag, or for which reporter molecules interfere with biological function. Field-effect sensing is a method of directly sensing intrinsic electrical charge associated with biomolecules without the need for reporter molecules. Microfabrication of field-effect biosensors enables their integration in compact microanalytical systems, as well as the potential to be scaled down in size and up in number. Applying field-effect sensing to the detection and real-time monitoring of specific molecular interactions has long been of interest for protein and nucleic acids analysis. However, these applications are inhibited by serious practical limitations imposed by charge screening in solution. The development of effective measurement techniques requires inquiry into aspects of device engineering, surface chemistry, and buffer conditions. This thesis describes a body of experimental work that investigates the feasibility of label-free analysis of biomolecular interactions by field-effect. This work begins with the microfabrication of field-effect sensors with extremely thin gate oxide, which enables improved surface potential resolution over previously reported sensors.
(cont.) The performance of these sensors has been characterized in terms of drift, noise, and leakage. To better understand the applicability of these sensors, we have characterized the sensors' response to pH, adsorption of polyelectrolyte multilayers, and high-affinity molecular recognition over a range of buffer conditions. Direct, label-free detection of DNA hybridization was accomplished by combining the high-resolution sensors, with enabling surface chemistry, and a differential readout technique. Finally, we explore the lateral scaling limits of potentiometry by applying a novel nanolithographic technique to the fabrication of a single electron transistor that demonstrates Coulomb oscillations at room temperature.
by Emily Barbara Cooper.
Ph.D.
Anderson, Henrik. "Development of Electroacoustic Sensors for Biomolecular Interaction Analysis." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-107211.
Повний текст джерелаRusso, Peter R. (Peter Raphael) 1980. "Integrated silicon field-effect sensors and microfluidics for biomolecular detection." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/17977.
Повний текст джерелаIncludes bibliographical references (p. 52-53).
Microfabricated silicon field-effect sensors with integrated poly(dimethylsiloxane) microfluidic channels have been demonstrated. These devices are designed for the label-free detection and recognition of specific biomolecules such as DNA. Label-free methods eliminate the time-consuming and costly step of tagging molecules with radioactive or fluorescent markers prior to detection. The devices presented here are sensitive to the intrinsic charge of the target molecules, which modulates the width of the carrier-depleted region of a lightly-doped silicon sensor. The variable depletion capacitance is precisely measured, indicating changes in sensor surface potential of less than 30[micro]V. The integrated microfluidic channels enable the delivery of small (nanoliter-scale) amounts of fluid directly to the sensors. Capacitance-voltage curves were recorded using phosphate buffered saline (PBS) as the test electrolyte; a maximum slope of 44pF/V was measured in depletion. pH sensitivity was also demonstrated using modified PBS solutions. A device with dual 80x80Om sensors yielded a response of 40mV/decade, referenced to the fluid electrode. A device with dual 50x50[micro]m sensors yielded a response of 12mV/decade, referenced to the sensors.
by Peter R. Russo.
M.Eng.
Zhang, Xiaojuan. "INVESTIGATION OF BIOMOLECULAR INTERACTIONS FOR DEVELOPMENT OF SENSORS AND DIAGNOSTICS." VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/294.
Повний текст джерелаTosolini, Giordano. "Force sensors based on piezoresistive and MOSFET cantilevers for biomolecular sensing." Doctoral thesis, Universitat Autònoma de Barcelona, 2013. http://hdl.handle.net/10803/131408.
Повний текст джерелаBiorecognition processes between receptors and their conjugate ligands are very important in biology. These biomolecules can build up very specific complexes displaying a variety of functions such as genome replication and transcription, enzymatic activity, immune response, cellular signaling, etc. The unambiguous one-to-one complementarity exhibited by these biological partners is widely exploited also in biotechnology to develop biosensors. Depending on the nature of the transduction signals, biosensors can be classified in optical, electrical and mechanical. Among mechanical biosensors, the microcantilevers play a prominent role. They have been used as stress or mass transducers in biomolecules detection for already more than a decade. The binding of molecules to their functionalized surface is detected by measuring either the deflection in static mode or the resonant frequency shift in dynamic mode. The deflection of the cantilever is converted optically by a laser and a photodetector in order to have the highest possible resolution. This limits the measurements in transparent liquids, the portability of the instrument and increases the complexity for multiplexing. The development of self-sensing cantilevers by integrating piezoresistors or metal-oxide-semiconductor field effect transistors (MOSFET) into the cantilever solves this issue. However, at the same time, this decreases the bending and frequency shift resolution due to the higher transducer noise. On the other hand, the detection of a single molecule can be attained measuring the unbinding force between two molecules of a complex pulling them apart, using the atomic force spectroscopy (AFS) measuring approach. This technique is based on the atomic force microscope (AFM). Despite the high force resolution, AFM has still not become an analytical instrument and it is mainly due to the complexity of the instrument and of its use. A biosensor based on AFS and on self-sensing cantilever would allow single molecule resolution, working in opaque fluids, easy multiplexing capability, and relatively easy integration in microfluidics cells. In this perspective, we worked to obtain self sensing-probes endowed with pN resolution and compatible with liquid media. Cantilevers based on single crystalline silicon have been modeled and the fabrication process has been optimized to improve the force sensitivity and to obtain high fabrication yield. At the same time we worked also on the modeling, development and fabrication of cantilevers with embedded MOSFET piezoresistive transducers. It turned out that the probes with integrated piezoresistor offer a more straightforward solution, but also the MOSFET cantilever can offer a good alternative. Alongside the force sensors fabrication, new high-throughput set-ups and techniques have been developed and optimized to measure the electrical and electromechanical characteristics of micro-electro-mechanical systems (MEMS) in a precise and reliable way. This was of key importance to correctly validate the new technological processes involved in production as well as characterize the final devices. After achieving very good sensor performances (resolution < 10 pN in liquid environment) with high production yield, we used the force probes to investigate the biorecognition processes in the avidin-biotin complex. For this purpose we integrated the sensor into a commercial AFM to take advantage of the high mechanical stability of this equipment and the highly reliable displacement of the piezo actuator. We detected the forces related to the avidin-biotin complex formation, highlighting the possibility of biomolecule label-free recognition in nearly physiological conditions and at single molecule resolution. Beside the very high sensitivity attained, the sensor can be used with no restrictions in opaque media; it can be easily integrated in microfluidic cells and it displays a high multiplexing potentiality. This result opens new perspectives in highly sensitive label free biomarkers detectors in nearly physiological conditions.
Weckman, Nicole Elizabeth. "Microfabricated acoustic sensors for the detection of biomolecules." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274899.
Повний текст джерелаDing, Shu Gu Li-Qun. "Aptamer encoded nanopores as single molecule sensors." Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/5767.
Повний текст джерелаJanczak, Colleen. "Hybrid Nanoparticles for Enhanced Sensitivity in Biological Labeling and Biomolecular Sensing." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202514.
Повний текст джерелаDe, la Rica Quesada Roberto. "New concepts for electrical detection of biomolecules." Doctoral thesis, Universitat Autònoma de Barcelona, 2007. http://hdl.handle.net/10803/3584.
Повний текст джерелаEn primer lloc, es presenta un nou tipus de transductor impedimetric (I). Es va escollir un disseny basat en dos electrodes interdigitats per dos motius principals. Primer, aquesta geometria permet monitoritzar tant la resistència como la constant dieléctrica d'una solució, la qual cosa fa dels electrodes interdigitats eines més versatils que altres tipus transductors. Segon, els electrodes presenten una curta penetració del camp electric, la qual cosa els fa mes sensibles als canvis que tenen lloc a prop de la seva superfície. Aquest fet permet monitoritzar canvis locals en les magnituds d'interés. Finalment, són apropiats no nomes per construir sensors sinó també actuadors. Aquesta geometria sembla ser útil en experiments de dielectroforesi. Una innovació introduïda en aquesta tesi es el material escollit per fabricar els electrodes: silici policristal-lí o polisilici. El polisilici pot ser facilment modificat per donar lloc a superficies amb particulars propietats químiques i físiques, fent d'aquest material un excel-lent candidat per a la manufactura de biosensors, comparable a altres aproximacions com la quemisorció de alcanotiols sobre electrodes d'or.
Els esmentats electrodes interdigitats es van fer servir per probar dos nous sistemes de transducció. Ambdues aproximacions comparteixen un tret comu: aprofiten la capacitat dels electrodes interdigitats per mesurar canvis local en les propietats elèctriques del medi on es troben submergits. En II, aquest fet és utilitzat per monitoritzar una reacció enzimàtica, i es mostra com la característica de mesura local en electrodes interdigitats dóna lloc a una detecció més sensible. A més, es demostra que aquesta aproximació es adequada per la detecció de proteïnes fent servir l'enzim com a marca en un immunoassaig. En III, els electrodes interdigitats actuen com a sensor i actuador. Com a actuador, els electrodes son capaços de concentrar esferes de làtex a la seva superficie. Com a transductors, la presencia de les micropartícules aïllants a la seva superficie dóna lloc a un canvi en la geometria de la cel-la, que pot ser detectat monitoritzant tant la resistència com la capacitat de la solucio. Aquest mode de funcionament es paral-lel al dels sensors magnetoresistius, i el principi de transduccio proposat es presenta com a una alternativa a ells.
Finalment, un quart treball es presenta en aquesta tesi (anex). Comparteix dues característiques en comú amb els treballs previs: el sustrat (silici) i una metodologia per la inmoblització de biomolecules (silanització). Les seves aplicacions son, però, diferents i cobreixen un rang més ampli d'aplicacions. En concret, una nova metodologia pel nanoestructurat de superfícies, de baix cost i fàcil disponibilitat és presentada. Es van aconseguir motius fets amb molècules de silà amb dimensions inferiors als 10 nm. En el marc de la biodetecció, aquesta nova tècnica per nanoestructurat superficial es propossa com a alternativa a la nanolitografia dip-pen per la manufactura de nanomatrius de biomolècules. Les petites dimensions dels motius obtinguts obren el cami per la consecució de nanomatrius d'una única molècula.
This work discusses different aspects related to the design of biosensors and biodetection systems. It describes the fabrication and characterization of particular electric transducers together with the development of new transduction systems and the finding of new methodologies for biomolecule nanoarray fabrication.
Firstly, a new type of impedimetric transducer is presented (I). A two-electrode interdigitated design was chosen, mainly for three reasons. First, this geometry allows the monitoring of both the resistivity and the dielectric constant of a solution, thus making interdigitated electrodes more versatile tools than other kind of transducers. Second, they present short electric field penetration depths, which make them more sensitive to changes occurring close to their surface. This fact enables the monitoring of local changes in the magnitudes of interest. Finally, they are suitable for constructing not only sensors but also actuators. This geometry appears to be useful in dielectrophoresis experiments. One innovation introduced in this thesis is the material chosen to fabricate the electrodes: polycrystalline silicon, also known as polysilicon. Polysilicon can be easily modified to render surfaces with distinct physical and chemical properties, thus making this material an excellent approach for biosensors manufacture, comparable to other approaches like alkanethiol chemisorption on gold electrodes.
The aforementioned interdigitated electrodes were used to test two new transduction principles. The two approaches share a common feature: they rely on the ability of interdigitated electrodes to measure local changes in the electrical properties of the medium where they are immersed. In II, this is used to monitor an enzymatic reaction, and it is shown that the characteristics of measuring local changes at interdigitated electrodes result in a more sensitive detection. Furthermore, the feasibility of this approach for protein detection is demonstrated by using the enzyme as a label for performing an immunoassay. In III, the interdigitated electrodes act both as a transducer and as an actuator. As an actuator, the electrodes are able to concentrate latex beads at their surface. As a transducer, the presence of the insulating microparticles at their surface results in a change in the geometry of the cell, that can be detected by monitoring either the resitance or the capacitance of the solution. Such device performance is parallel to that of magnetoresistive biosensors, and the proposed transduction principle is envisaged as a suitable alternative to them.
Finally, a fourth work is presented in this thesis (Annex). It shares two features in common with the previous works: the substrate (silicon) and a method for biomolecule immobilization (silanization). However, the applications are somehow different, and cover a wider range. Precisely, a new methodology for low cost, easily available nanopatterning is shown. Features made of silane molecules, with dimensions less than 10 nm are successfully patterned. In the frame of biodetection, this new nanopatterning technique is proposed as an alternative to dip-pen nanolithography in nanoarray manufacture. Moreover, the small dimensions of the obtained patterns pave the way for the achievement of single-molecule nanoarrays.
Herland, Anna. "Conjugated Polymers, Amyloid Detection and Assembly of Biomolecular Nanowires." Doctoral thesis, Linköping : Biomolecular and Organic Electronics, Department of Physics, Chemistry and Biology, Linköping University, 2007. http://www.bibl.liu.se/liupubl/disp/disp2007/tek1117s.pdf.
Повний текст джерелаXu, Liang. "Giant magnetoresistive sensor for biomolecule detection and cancer diagnosis /." May be available electronically:, 2009. http://proquest.umi.com/login?COPT=REJTPTU1MTUmSU5UPTAmVkVSPTI=&clientId=12498.
Повний текст джерелаKyprianou, Dimitris. "Development of novel matrices for biomolecule immobilisation on sensor surfaces." Thesis, Cranfield University, 2010. http://dspace.lib.cranfield.ac.uk/handle/1826/4490.
Повний текст джерелаBranquinho, Rita. "Label-free detection of biomolecules with Ta2O5-based field effect devices." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/9413.
Повний текст джерелаBane, Danielle Nichole. "A Resonant Capacitive Test Structure for Biomolecule Sensing." University of Dayton / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1437658452.
Повний текст джерелаJönsson, Mats. "Microfluidic Devices for Manipulation and Detection of Beads and Biomolecules." Doctoral thesis, Uppsala universitet, Institutionen för teknikvetenskaper, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6746.
Повний текст джерелаTan, Yi Lei. "Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/289718.
Повний текст джерелаChao, Christina. "Étude des propriétés émollientes de biomolécules commerciales et synthétisées en vue de la substitution du décaméthylcyclopentasiloxane (D5)." Thesis, Compiègne, 2017. http://www.theses.fr/2017COMP2379/document.
Повний текст джерелаEmolliency is a word used to define the ability of a compound to soften or lubricate the skin. ln the cosmetic field, emollients are used to modify the consistency, the viscosity or the polarity of a formulation. Many emollients can be used in cosmetic products. However, in the literature both physicochemical and sensory data ar still lacking, making it difficult to choose an emollient. Furthermore, the sensory analysis usually performed to characterize emollients are particularly time-consuming and thus, expensive. Among the different chemical families of emollients, silicone derivatives stand out thanks to their specific properties. Indeed, they are characterized by an excellent spreading on skin and hair, a smooth skin feel, non-greasy and non-sticky, or by a dry skin feel without a fresh effect. However, even though these sensory properties are exceptional, recent studies wonder about the toxicity of a cyclic silicone particularly used in cosmetic products: the decamethylcyclopentasiloxane (D5). Thus, this work deals With two main objectives. The first one consists in the research of a bio-based alternative to the D5 For this purpose, a number of commercial and synthesized molecules were characterized and compared With physicochemical measurements and sensory analysis, allowing the observations of trends between structures and properties. The second objective relies on the study of correlations between physico-chemical and sensory data in order to predict the emollient properties of cosmetic ingredients. This would ease the work of formulators during the screening of ingredients
Jalal, Ahmed Hasnain. "Multivariate Analysis for the Quantification of Transdermal Volatile Organic Compounds in Humans by Proton Exchange Membrane Fuel Cell System." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3886.
Повний текст джерелаHu, Wen-Pin, and 胡文品. "Kinetics study and conformation analysis of biomolecular interactions by applying surface plasmonic sensors." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/61470194271574932065.
Повний текст джерела國立成功大學
醫學工程研究所碩博士班
95
With the suitable immobilizations of biomolecules on the sensing surface of surface plasmon resonance (SPR) sensors, they can be extended as useful tools in the studies of biomolecular interactions. In this dissertation, the study of β-amyloid (Aβ) peptide aggregation, the immunodetection of C-reactive protein (CRP), and the fabrication of a multispot DNA chip were evaluated by the SPR sensing or imaging techniques which constructed the three specific applications. The aim of first application is to observe the process of Aβ aggregation with or without the existence of metal ions. The immunodetection of CRP is to employ SPR biosensor in the measurement of pentamer and modified CRPs with less false signals. Finally, a practical method to fabricate a multispot DNA chip is proposed and evaluated by a SPR imager. Soluble Aβ(1-40) peptide is immobilized on the surface of SPR chip and the aggregating reaction occurs spontaneously or under the induction of metal ions. A first-order kinetics model is applied to analysis the data for getting the kinetic parameters. A metal chelator, EDTA, is used in the experiments for testing its effect on the disruption of Aβ aggregates induced by metal ions. Results revealed the metal ions promoted Aβ aggregation with various propensities. Cu(II) could induce a rapidest initial Aβ aggregation, but it did not promote the formation of large Aβ aggregates. The Aβ aggregates induced by metal ions could be disrupted by the chelator, EDTA. For constructing surface with a well-order immobilization of antibodies, the three monoclonal antibodies (Mabs), C8, 8D8, and 9C9 are immobilized on a protein G layer in the immunodetection of CRP. In all experiments of detecting CRP, no false results were observed in the recognition of modified and pentamer CRPs. In order to prepare a multispot DNA chip, thiolated single-stranded DNAs (ssDNAs) (1 μM) with short sequences and oligo (ethylene glycol) (OEG) alkanethiol (50 μM) mixed in 1 M KH2PO4 is used to spot on the SPR sensing chip. The commercial program, Oligo, is applied to calculate of possibility on formation of DNA secondary structure. The experiments of DNA hybridization are performed at two different temperatures for interpreting the effect of temperature on DNA hybridization. According to the results from the DNA hybridizing experiments evaluated on the SPR imaging system, the fabrication method for making a multispot DNA chip was proved its feasibility. The effect of DNA secondary structure on hybridization was verified that could be minimized by raising experimental temperature. The experimental resluts obtained from the SPR techniques are easily affected by chemical surface modifications and biomolecular immobilizations on the sensing surface. The several methods used in this dissertation are successfully applied in the experiments, and they also can be used in other biomolecular detections. The experimental results can provide the useful examples of applying SPR techniques in the biomedical examinations to other related researchers.
Peksa, Vlastimil. "Designing and testing of new metal nanosubstrates for biomolecular sensors based on surface-enhanced Raman scattering (SERS) spectroscopy." Doctoral thesis, 2017. http://www.nusl.cz/ntk/nusl-367962.
Повний текст джерелаKumawat, Nityanand. "Self-referencing Techniques in Optical Label-free Bio-molecular Sensing." Thesis, 2013. https://etd.iisc.ac.in/handle/2005/4577.
Повний текст джерелаHuang, Yu-cheng, and 黃裕成. "Design of a Portable Multi-Channel Potentiostat for Biomolecule Sensors." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/74703907496591192028.
Повний текст джерела國立臺南大學
通訊工程研究所碩士班
98
In this thesis, two portable multi-channel potentiostats are proposed for the signal processing of electrochemical biosensors. One is designed by modular approach to construct a portable multi-channel potentiostat and the other is directly designed to implement a portable multi-channel auto-range potentiostat. The proposed potentiostats can perform three operation modes which are commonly used in electrochemical experiments: potentiometry, voltammetry, and cyclic voltammetry. In system verification, taking advantage of the function of cyclic voltammetry, the proposed potentiostats are used to integrate an EGCG sensor and a caffeine sensor to measure EGCG and caffeine concentrations in green tea. The experimental results show that the biosensor currents measured by the proposed potentiostats have the same trend as those measured by the commercial potentiostats when the EGCG concentration and the caffeine concentration change. According to the calibration curve, the measured caffeine in green tea can reach 101.3%、93.25% and 97.27% accuracy respectively for three different green tea samples respectively. As for the EGCG measured, the results are 111.19%、100.53% and 122.66% accurate in each sample. In conclusion, the proposed multi-channel potentiostats have the merits of small size, light weight, high portability, low cost, and being able to measure multi-biosensors at the same time. In the future, we will integrate the proposed potentiostats with biosensors to develop the home-care system in daily life.
Nemati, Mahdieh. "Development of optical microchip sensor for biomolecule detection." Thesis, 2018. http://hdl.handle.net/2440/114272.
Повний текст джерелаThesis (Ph.D.) (Research by Publication) -- University of Adelaide, School of Chemical Engineering, 2018
Tsou, Pei-Hsiang. "Porous Membrane-Based Sensor Devices for Biomolecules and Bacteria Detection." Thesis, 2012. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11878.
Повний текст джерелаChen, Fa-Chun, and 陳法均. "The effect of design/fabrication factors in nanowire biomolecules sensor." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/87261419215690191672.
Повний текст джерела國立臺灣大學
電子工程學研究所
100
In recent years, the use of nanowire transistors as a new bio-molecular measurements device has became a good tool in the detection of the disease. This new biosensor could measure bio-molecular signals in a short time, also provides the advantages of high sensitivity, high selectivity, small size and probability to mass production. However, there are still some problems in this new technology need to be overcome, especially the stability and sensitivity characteristics which are the two most widely discussed. The improved methods can be broadly divided into three types, one is bio–abio interface, one is the device structure improvements, and the last one is fabrication technique. There are two parts of discussion in this paper, the first discussion is to discuss the different structures consists of single-nanowire, multiple-nanowires and ribbon- nanowire. The second discussion is to discuss the difference between heavy ion implantation in S/D device and normal ion implantation device. Both discussions are focused on the comparison of the different bio-molecular sensing properties in these two devices. To verify the purpose of this thesis wants to achieve, the various devices are fabricated and experimentally verified. The silicon nanowire thin-film transistor is bio-functionalized to have the specific-binding to testing DNA strands, so the DNA concentration of the test. Then we use the change of the threshold voltage to analyze the sensitivity and stability of bio-molecular sensor of silicon nanowire transistors. By the two analytical results, this paper can clearly demonstrate the effect of the comparison of different structure and different ion concentration in biomolecular sensing.
FOTI, ANTONINO. "SURFACE- AND TIP- ENHANCED RAMAN SPECTROSCOPY OF BIOMOLECULES." Doctoral thesis, 2017. http://hdl.handle.net/11570/3103680.
Повний текст джерелаPan, Chien, and 潘杰. "Determination of small biomolecule by fiber-optics surface plasma resonance sensor." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/7a9z8r.
Повний текст джерела大同大學
生物工程學系(所)
95
Fiber-optic surface plasmon resonance (SPR) can be used as a rapid and quantitative assay, and is also an important tool for monitoring biomolecular interactions. Furthermore, the use of fiber-optic SPR provides a number of potential advantages. Because the fiber optic sensors are reproducible and inexpensive to be disposable, binding kinetics and screening analyses can be performed in complex media without fear of irreversibly fouling expensive chipbased SPR platforms. Additionally, the small size of the fibers optic probe and sensing area make the sensors ideal for studying small volumes of samples. The applications are more and more than before. In this study, we used the fiber-optic SPR sensor designed and obtained from Graduate Institute of Electro-Optical Engineering in Tatung University to determine the small biomolecules. The nanogold particle and precipitation of 4-choro-1-naphthol were used to amplify the detection signals of SPR. The amplification method using nanogold particle was insignificant, but one using precipitation by reducing 4-choro-1-naphthol was able to enhance significantly the SPR signals. The signal changes of wavelength shift were consistent to the increasing in the concentrations of biotin-conjugated albumin bovine serum (biotin-BSA) and ochratoxin A-conjugated ovalbumin (OTA-OVA). Therefore, these results indicated that the amplification method of reducing precipitation by enzyme was suitable to the fiber-optic SPR detection system. The detection limiting for biotin-BSA was 0~10 �慊/ml, and 10~20 ng/ml for biotin.
Tu, Yu-chieh, and 涂煜杰. "Surface Immobilization of Biomolecules onto Plasma Treated and Surface Grafted Acrylamide for Humidity sensors." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/vytzhg.
Повний текст джерела大同大學
材料工程學系(所)
95
The plasma deposition and surface grafting polymerization have been used to gradually formed the organic layers on inorganic substrates such as interdigital electrodes devices for single strand DNA immobilization. In this study, the semiconductive tin oxide (SnOX) organic-like thin films were deposited by PECVD of TMT and O2 mixtures on the comb-shaped electrodes as the humidity sensor devices. To improve the sensitivity and stability for humidity sensing, the surface of the deposited flims was subsequently grafted with AAm (acrylamide). Hence, GA as cross-linking reagent to chemical bonding NH2-oligonucleotide probes on the surface of interdigital electrodes. As the surface of sensitive electrodes was immobilized the single strand DNA fragment of Vibrio parahaemolyticus or antibody, ionic molecules, etc. The grafted ionic molecules film enhances the impedance variations with humidity. When surface immobilization of antigen, the results appear the best sensitive range for humidity is R.H. 35∼95%, and the impedance variations decreases by over 5 orders of magnitude from 107 to 102 W .
Zhao, Wei. "Evaluation of Zinc Oxide Nano-Microtetrapods for Biomolecule Sensing Applications." Thesis, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-181790.
Повний текст джерелаWang, Xiaomeng. "Surface-attached Biomolecules and Cells Studied by Thickness Shear Mode Acoustic Wave Sensor." Thesis, 2008. http://hdl.handle.net/1807/17248.
Повний текст джерела"Top-Down and Bottom-Up Strategies to Prepare Nanogap Sensors for Controlling and Characterizing Single Biomolecules." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.54953.
Повний текст джерелаDissertation/Thesis
Doctoral Dissertation Physics 2019
(9029573), Carmen Gondhalekar. "Laser-induced breakdown spectroscopy applications for metal-labeled biomolecule detection in paper assays." Thesis, 2020.
Знайти повний текст джерелаChen, Liang-Yu, and 陳良宇. "Biomolecules Interaction Analysis With Biosensors:I. Monitoring the Conformational Changes of Immobilized Proteins and Evaluation of Measurement Uncertainty II. The Fabrication of Mimetic Lipid Bilayer on Sensor Surface and Its Application." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/79336214965857916588.
Повний текст джерела國立中央大學
化學工程與材料工程研究所
93
Biological events are initiated by bio-molecular interactions, e.g. protein-protein, protein-nucleic acid, protein-lipid and chemical ligand-receptor interactions. The kinetics, thermodynamics and conformational matching involved in the contact between two or more molecules determine whether the interaction has any biological meaning in the real world. To clearly characterize the process of molecular interactions and further explore their true behaviors in biological systems, highly sensitive, specific, precise and fast biosensors are required to obtain the real-time and quantifiable information of interactions. In this study, we investigated the application of surface plasmon resonance sensor (SPR) in the bio-molecular interactions analysis. The core of molecular recognition lies on the study on immobilized bio-molecule conformation, which is a very challenging research field. In the regard of uncertainty analysis of SPR, we evaluated the source and composition of signals of angle measurements and established the principle of data analysis. Based on this, we disclosed the effect of interactions between bulk solution, solute and immobilized matrix on measurement. Further more, the direct observation of conformational changes of immobilized proteins in unfolding and refolding derived a lot of important structural information of protein, such as the compactness of conformations, the exposure of hydrophobic region and the effects of intrinsic disulfide bonds on structural stability of immobilized proteins. On the other side, the combination of real-time monitoring capacity of QCM and microscopic surface morphology by AFM was employed to investigate the effects of liposome composition on the structure of supported lipid bilayer and the kinetics mechanism of absorption at the solid-liquid interface. The formula of stable vesicular lipid bilayer was established. We also demonstrated the possibility of application of vesicular lipid bilayer in biophysical and biochemical studies by the transmembrane process of melittin. We believe that the mimetic membrane systems would be ideal platform candidates to study bio-molecules interactions in the application of biosensors.