Добірка наукової літератури з теми "Bioinoculants"

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Статті в журналах з теми "Bioinoculants"

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Ambawade, M. S., N. V. Manghwani, P. R. Madhyani, A. M. Shaikh, D. D. Patil, and G. R. Pathade. "Influence of Yeast Bioinoculant Isolated from Indian Date Palm Tree (Phoenix sylvestris) Sap on the Health of Wheat Crop and Soil." Nature Environment and Pollution Technology 22, no. 4 (December 1, 2023): 2093–101. http://dx.doi.org/10.46488/nept.2023.v22i04.034.

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In this study, three promising yeast isolates were isolated from the sap of the Indian date palm tree (Phoenix sylvestris) and characterized by biochemical tests and 18S rRNA gene sequencing. They were confirmed as Saccharomyces cerevisiae and were designated as strains PYS-1, PYS-2, and PYS-3. These confirmed strains were used for the preparation of bioinoculants. Bioinoculant was prepared and applied to wheat crops, and the effect of Bioinoculant. Statistical analysis is carried out using analysis of variance (ANOVA), and it is found that the absorbance of chlorophyll, protein, and Indole Acetic Acid (IAA) content is significantly increased. The treatment of bioinoculant showed that crops significantly increased chlorophyll, protein, and IAA content. Further, we applied bioinoculant on the soil and measured the soil’s humus content before and after the treatment of bioinoculant. Then, a paired t-test was applied to check the effectiveness of the treatment, and it was found to significantly increase humus content in the soil. The use of bioinoculants is an economically feasible and eco-friendly method.
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Nosheen, Asia, Asghari Bano, and Faizan Ullah. "Bioinoculants." Toxicology and Industrial Health 32, no. 2 (October 4, 2013): 270–77. http://dx.doi.org/10.1177/0748233713498453.

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Chaudhary, Twinkle, and Pratyoosh Shukla. "Bioinoculant capability enhancement through metabolomics and systems biology approaches." Briefings in Functional Genomics 18, no. 3 (June 20, 2018): 159–68. http://dx.doi.org/10.1093/bfgp/elz011.

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AbstractBioinoculants are eco-friendly microorganisms, and their products are utilized for improving the potential of soil and fulfill the nutrients requirement for the host plant. The agricultural yield has increased due to the use of bioinoculants over chemical-based fertilizers, and thus it generates interest in understanding the innovation process by various methods. By gene-editing tool, the desired gene product can be changed for engineered microbial inoculants. We have also described various modern biotechnological tools like constraint-based modeling, OptKnock, flux balance analysis and modeling of the biological network for enhancing the bioinoculant capability. These fluxes give the fascinating perception of the metabolic network in the absence of comprehensive kinetic information. These tools also help in the stimulation of the metabolic networks by incorporation of enzyme-encoding genes. The present review explains the use of systems biology and gene-editing tools for improving the capability of bioinoculants. Moreover, this review also emphasizes on the challenges and future perspective of systems biology and its multidisciplinary facets.
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Akshitha, H. J., K. Umesha, and T. H. Shankarappa. "Effects of Type of Cutting, IBA and Bioinoculants on Rooting in Madhunashini (Gymnema sylvestre Retz.)." Journal of Horticultural Sciences 9, no. 1 (June 30, 2014): 94–97. http://dx.doi.org/10.24154/jhs.v9i1.232.

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An experiment was carried out to study the effect of type of cutting, IBA and bioinoculants on rooting in madhunashini. Among the three types of cuttings, hardwood cuttings registered higher values for fresh (0.790g/cutting) and dry weight (0.650g/cutting) of sprouts, per cent rooting (6.66 %), fresh and dry weight of roots (0.037 and 0.030g/ cutting) and biomass production (0.682g/cutting). Among IBA and bioinoculant treatments, Azotobacter chroococcum recorded higher values for percentage sprouting (26.66 %) and rooting (9.99 %) as also for other root parameters; whereas, maximum fresh weight (0.863g/cutting) and dry weight of sprouts (0.740g/cutting), and, biomass production (0.759g/cutting) was observed in IBA 1000ppm treatment. Interaction effect of type of cutting, IBA and bioinoculants on fresh and dry weight of sprouts (2.438g and 2.084g, respectively) and biomass production (2.123g/cutting) was found superior in hardwood cuttings treated with IBA 1000ppm. Percentage of rooting (13.33 %) was better in hardwood cuttings treated with Azotobacter chroococcum. Therefore, among the various treatments tested, hardwood cuttings treated with Azotobacter chroococcum are the best for propagation through cuttings.
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Berdeja, Mariam P., Qiuhong Ye, Taryn L. Bauerle, and Justine E. Vanden Heuvel. "Commercial Bioinoculants Increase Root Length Colonization and Improve Petiole Nutrient Concentration of Field-grown Grapevines." HortTechnology 33, no. 1 (February 2023): 48–58. http://dx.doi.org/10.21273/horttech05110-22.

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Agricultural bioinoculants containing arbuscular mycorrhizal fungi represent a potential opportunity to reduce the dependence of grapevines (Vitis) on agrochemicals. This field study assessed the ability of four commercial bioinoculants to colonize grapevine roots and their effects on petiole nutrient concentration, berry composition, and root morphology of ‘Pinot noir’ (Vitis vinifera) grafted onto rootstock ‘Couderc 3309’ (Vitis riparia × Vitis rupestris) and ‘Riesling’ (V. vinifera) grafted onto ‘Couderc 3309’ and Selection Oppenheim four (Vitis berlandieri × V. riparia). Three bioinoculants increased root mycorrhizal colonization; however, regardless of the treatment, mycorrhizal fungal structures were enhanced. Grapevine petiole nutrient concentration was improved by bioinoculants. Root diameter, root length density, and specific root length increased with greater mycorrhizal root colonization. Using bioinoculants to reduce chemical fertilizers may be a good strategy to improve grapevine productivity and health in cool climates; however, the impact of mycorrhizal bioinoculants in the vineyard may differ among scion–rootstocks, edaphoclimatic conditions, and vineyard soil microbiomes.
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K.S, RAGHUWANSHI, PAWAR K.B, and PATIL J.D. "STANDARDIZATION OF LOAD OF BIOINOCULANT IN PEARLMILLET UNDER DRYLAND CONDITIONS." Madras Agricultural Journal 85, september (1998): 355–57. http://dx.doi.org/10.29321/maj.10.a00751.

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Field trial was conducted to study the response of pearlmillet to differnt loads of bioinoculants on vertisol for six years in dryland conditions. Dual inoculation of Azospirillum lipoferum and Azotobacter chroococcum at optimum load of inoculum i.c. 60 g/kg seed performed economically well in respect of plant height, 1000 grain weight, Nitrogen uptake besides grain and stover yields over their individual inoculaiton at recommended dose i.c. 30 g/kg seed. More cost benefit ratio and additional net income was recorded by 60 g/kg seed treatment as compared to recommended dose of bioinoculant.
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Kandula, D. R. W., H. Alizadeh, C. S. P. Teixiera, D. Gale, A. Stewart, and J. G. Hampton. "Trichoderma bioinoculant improves seedling emergence plant growth and seed yield of Camelina sativa (L) Crantz." New Zealand Plant Protection 68 (January 8, 2015): 160–65. http://dx.doi.org/10.30843/nzpp.2015.68.5835.

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Camelina sativa seedling emergence and plant growth can be reduced by the soilborne pathogen Rhizoctonia solani The effect of a mixture of Trichoderma atroviride isolates applied in granule and prill formulations at a rate equivalent to 15 kg/ha on two varieties of C sativa (Suneson and 4164) was evaluated in glasshouse experiments using R solani infested soil The bioinoculant treatments significantly increased seedling emergence by 3060 and increased total shoot and root dry weight by 48 fold over that of the untreated control In a field experiment in 20122013 using C sativa variety 4164 the prill formulation of the bioinoculant was applied at sowing Seedling emergence was not significantly increased but shoot dry weight was increased by 20 and seed yield was increased by 25 by the bioinoculant treatment over that of the control Trichoderma bioinoculants may have a role in the integrated crop management of this second generation biofuel crop
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Selno, Silvester, Zulfa Zakiah, and Rikhsan Kurniatuhadi. "Kualitas Gaharu Aquilaria sp. dengan Pemberian Bioinokulan Fermentasi Batang Pisang yang Terkena Penyakit Layu Fusarium." JURNAL BIOS LOGOS 11, no. 2 (April 12, 2021): 94. http://dx.doi.org/10.35799/jbl.11.2.2021.32551.

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(Article History: Received February 1, 2021; Revised March 8, 2021; Accepted April 7, 2021) ABSTRAK Upaya produksi gaharu budidaya umumnya menggunakan inokulan dari jenis Fusarium sp., namun mahalnya inokulan biakan murni menjadi faktor pembatas untuk produksi gaharu budidaya. Bioinokulan dapat menjadi solusi bagi petani gaharu karena dibuat dari bahan yang mengandung mikroorganisme patogen dan tidak membutuhkan proses pembiakan murni. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis mikroorganisme dan konsentrasi bioinokulan yang tepat digunakan untuk menghasilkan kualitas gaharu yang baik serta mengetahui kualitas gaharu yang dihasilkan dengan menggunakan bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium. Metode penelitian lapangan dengan melakukan inokulasi bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium pada pohon Aquilaria sp. Setiap konsentrasi diinokulasikan pada pohon yang berbeda. Pada ketinggian 30 cm dari permukaan tanah, pohon dibuat lubang sebanyak 5 buah secara vertikal dengan menggunakan bor dengan jarak antar lubang 50 cm. Inokulan diinokulasikan melalui lubang yang telah dibuat dengan metode infus. Hasil penelitian diperoleh tiga jenis mikroorganisme yang terdapat pada bioinokulan yaitu kelompok genus Aspergillus, Fusarium dan Saccharomyces. Perlakuan dengan konsentrasi bioinokulan 140 ml memberikan hasil yang terbaik terhadap bobot kayu gaharu, warna, aroma dan kadar resin gaharu yang dihasilkan. Berdasarkan penelitian ini dapat disimpulkan bahwa kualitas gaharu yang dihasilkan setelah 4 bulan inokulasi menghasilkan mutu gaharu kelas kemedangan. Kata Kunci: Aquilaria sp., Bioinokulan, Fusarium sp., Gaharu, Inokulasi ABSTRACT Efforts to produce cultivated agarwood generally use inoculants of the type Fusarium sp. however the high cost of pure culture inoculants is a limiting factor for cultivated agarwood production. Bioinoculants can be a solution for agarwood farmers because they are made from materials containing pathogenic microorganisms and do not require a pure breeding process. The research aims to determine the kind of microorganisms and the proper concentration of bioinoculants to produce good quality agarwood and determine the quality of aloes produced by using bioinoculants from fermented banana stem affected by fusarium wilt disease. The method of field research was by conducting inoculation of bioinoculants from the fermentation of banana stems affected by fusarium wilt on Aquilaria sp. Each concentration was inoculated on a different tree. At a height of 30 cm from the ground, 5 holes are made vertically using a drill with a distance of 50 cm between the holes. The inoculant is inoculated through the hole that has been made by the infusion method. The results showed that there were three types of microorganisms found in bioinoculants, namely the Aspergillus, Fusarium and Saccharomyces genus groups. Treatment with a concentration of 140 ml of bioinoculant gave the best results on the weight of agarwood, color, aroma and content of the resulting agarwood resin. Based on this research, it can be concluded that the quality of aloes produced after 4 months of inoculation resulted in the quality of agarwood in the kemedangan class.Keywords: Agarwood, Aquilaria sp., Bioinoculants, Fusarium sp., Inoculation
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Chakravarty*, Gargi. "Preparation of Plant Growth Enhancing Bioformulation from Agricultural Wastes by Solid State Fermentation." Current Agriculture Research Journal 12, no. 1 (April 20, 2024): 316–25. http://dx.doi.org/10.12944/carj.12.1.25.

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Preparation of organic waste based bioformulation through solid state fermentation technology for application in the field offers an ecofriendly and viable alternative to sustainable food production. The present study deals with the potential of seven agrowastes as substrate carriers for the growth of indigenous strains of Pseudomonas fluorescens (Pf) and Bacillus subtilis (Bs) that stimulate plant growth. The cosubstrate (CS) with equal proportion of the seven agrowastes and both the bioinoculants (CSPfBs) proved as the best substrate carrier with highest population load of the bioinoculants at 45 days after storage (DAS). Thereafter the population declined steadily in all the bioformulations from 60 DAS to 90 DAS. However, CSPfBs gave the highest population load at 90 DAS. As individual substrate for both Pf and Bs, vermicompost performed best with a population load of 9.95 of Pf and 9.98 of Bs at 90 DAS. The bioformulations CSPfBs, VBs and VPf when applied in soil, gave the best yield in brinjal plants (Solanum melongena L. cv. Pusa Kranti) at 30 days after transplanting. Similar trends were also observed in the other yield parameters. The study indicates the scope of the prepared bioinoculant as a low cost eco friendly option for promotion of growth and yield of crops.
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A. N. Ravindranath and U. S. Sarma. "BIOINOCULANTS FOR COIR RETTING." CORD 11, no. 01 (June 1, 1995): 34. http://dx.doi.org/10.37833/cord.v11i01.287.

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The mesocarp of the coconut or its husk is the source of coir. Coir fibre has several potential end uses like for the manufacture of various coir products predominantly coir floor coverings, yarn, rope, rubberized coir and latest as geotextile material. Coir is extracted from the coconut husk which is subjected to steeping in saline backwa­ters for six to nine months. This prolonged period of steeping is disadvantageous on economic considerations and also deprives the coir industry of sufficient raw material for the product sector. The treatment of selected strains of phenol de­grading bacterial cultures on to husks immersed for retting is reported to reduce the period of ret­ting to some extent 3. This paper reports the find­ings of a field study on the treatment of selected strains of bacteria on one lakh husks steeped for retting. Samples of husks drawn out after three months of steeping were observed to be fully retted and yielded fibre suitable for spin­ning superior quality coir yarn.
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Дисертації з теми "Bioinoculants"

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Bhattacharjee, Priyanka. "Molecular detection and diversity analysis of bipolaris sorokiniana (Sacc) shoemaker and induction of resistance in sorghum bicolor (L) moench using bioinoculants." Thesis, University of North Bengal, 2018. http://ir.nbu.ac.in/handle/123456789/2725.

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Acharya, Amrita. "Serological and molecular detection of foliar fungal pathogens of Persea bombycina Kost and activation of defense response using bioinoculants." Thesis, University of North Bengal, 2016. http://ir.nbu.ac.in/hdl.handle.net/123456789/2767.

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Batista, Bruna Durante. "Promoção de crescimento vegetal por Bacillus sp. RZ2MS9: dos genes ao campo." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-15082017-170543/.

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Para alimentar a população mundial crescente é necessário um aumento sustentável na produtividade agrícola. Nesse sentido, Rizobactérias Promotoras de Crescimento de Plantas (RPCPs) têm sido continuamente buscadas para formulações inoculantes por sua capacidade de incremento na produção vegetal aliado ao seu potencial de redução e/ou substituição do uso de fertilizantes minerais, insumos que causam grandes impactos ambientais, na saúde humana e econômicos. A RPCP Bacillus sp. RZ2MS9, um representante da biodiversidade amazônica brasileira, é uma forte candidata a bionoculante por seu efeito benéfico, previamente descrito, em uma ampla gama de culturas, incluindo milho e soja. Essas duas culturas representam mais de 80% da área cultivada com grãos no Brasil, de forma que incrementos relativamente modestos de crescimento e produtividade poderiam gerar ganhos significativos. Membros do gênero Bacillus apresentam vantagem em formulações inoculantes, principalmente devido a sua capacidade de formação de esporos resistentes ao calor e dissecação. Seus modos de ação são diversos, tornando o entendimento da sua interação com plantas bastante desafiador. Bacillus sp. RZ2MS9 apresentou, dentre os mecanismos envolvidos na promoção de crescimento vegetal, a produção de Ácido Indol Acético (AIA) e sideróforos, solubilização de fosfato e fixação biológica de nitrogênio, in vitro. No presente trabalho, foi buscado um entendimento detalhado dos mecanismos de ação dessa rizobactéria, explorando desde seu genoma até seu desempenho em condições de campo. O draft genômico (genoma parcial) bacteriano foi obtido utilizando a tecnologia de sequenciamento Illumina, o qual possibilitou a detecção de genes envolvidos nos mecanismos potencialmente relacionados ao efeito benéfico dessa bactéria, que vão desde sua formação de esporos, atração por exsudatos radiculares, motilidade e competição na rizosfera até mecanismos de solubilização de fosfato, produção de sideróforos, entre outros. As informações obtidas permitem uma exploração genética desses mecanismos, fornecendo uma oportunidade de maximizar essa interação e, futuramente, favorecer os benefícios em campo. Adicionalmente, foi demonstrado o potencial de quimiotaxia (atração) de RZ2MS9 em direção a raízes de milho. Um estudo filogenético dessa RPCP, utilizando um método de tipagem com o gene pycA (piruvato carboxilase), mostrou que o Bacillus sp. RZ2MS9 apresentou-se distante do clado altamente monomórfico de B. anthracis, patógeno humano, e se afiliou a um grupo composto por linhagens de B. thuringiensis (Bt) comercializadas como produtos biopesticidas há mais de 60 anos, o que sugere a potencial possibilidade de seu uso seguro no campo. Sabe-se que a maioria, se não todas, atividades fisiológicas das plantas é regulada por fitormônios como a auxina AIA, os quais podem ser sintetizados também por RPCPs. Com mais detalhamento, os genes envolvidos nas vias biossintéticas desse fitormônio foram detectados no draft genômico de RZ2MS9, indicando que sua produção ocorre através da via IPA (Indol-3-Piruvato). Além disso, plantas de tomate anão Micro-Tom (MT) e seu mutante Δdgt, defectivo na sensibilidade a auxinas, foram utilizadas para caracterizar especificamente o efeito do AIA produzido por Bacillus sp. RZ2MS9 na promoção de crescimento vegetal. A aplicação de RZ2MS9 causou inibição no crescimento de raízes primárias, aumento no comprimento de raízes laterais e na área superficial total de raízes de plantas MT, efeitos característicos daqueles proporcionados por auxinas. Esse incremento radicular refletiu, ainda, em aumento da biomassa da parte aérea de plantas MT. Os mesmos efeitos não foram observados em plantas Δdgt, insensíveis a auxinas, indicando que a elicitação de promoção de crescimento em MT por RZ2MS9 ocorre por meio desses fitormônios. Finalmente, foi demonstrado o efeito sobre o desenvolvimento e produtividade de milho e soja da aplicação de Bacillus sp. RZ2MS9 em condições de campo, sendo comparado com o desempenho de bioinoculantes comerciais. No milho, o efeito da inoculação bacteriana foi, ainda, associado à adubação nitrogenada para verificar a possibilidade de redução desses insumos. Bacillus sp. RZ2MS9 apresentou efeitos significativos sobre o desenvolvimento tanto da soja (comparáveis aos efeitos de rizóbios) quanto do milho, os quais, porém, não refletiram em aumento significativo de produtividade em ambas as culturas. No entanto, o potencial dessa rizobactéria é bastante claro pois, com um custo de produção inferior a R$1,00 por hectare, sua inoculação causou incremento de 16 sacas de milho por hectare com redução de 30% na adubação nitrogenada, assim como um incremento de 11 sacas de soja por hectare, ambos comparados ao controle não inoculado. Os resultados apresentados no presente trabalho vão, portanto, de encontro à grande expectativa na obtenção de linhagens microbianas promissoras visando sistemas agrícolas mais sustentáveis.
To feed the growing global population, a sustainable increase of agricultural production and crop yield is required. In this sense, Plant Growth Promoting Rhizobacteria (PGPR) have been continuously sought to inoculant formulation due to their capacity to increase plant yield along with their potential to reduce and/or replace the use of mineral fertilizers, inputs that cause serious impacts on environment, human health and economy. The PGPR Bacillus sp. RZ2MS9, a representative of the Brazilian Amazonian biodiversity, is a great candidate to bioinoculant because of its beneficial effect on a broad range of crops, including maize and soybean. These two crops represent more than 80% of the area planted with grains in Brazil, so relatively modest growth and yield increases could generate significant gains. Bacillus spp. have advantage in inoculant formulations, mainly due to their ability to form heat- and dissecation-resistant spores. Their modes of action are diverse, making the understanding of its interaction with plants quite challenging. Bacillus sp. RZ2MS9 displays, between the mechanisms involved in plant growth, Indole Acetic Acid (IAA) and siderophore production, phosphate solubilization and biological nitrogen fixation, in vitro. In the present work, we seek a detailed understanding of this rhizobacterium mechanisms of action, exploring from its genome to its performance in field conditions. The bacterial draft genome was obtained using Illumina sequencing technology, making possible the detection of genes involved in mechanisms potentially related to the beneficial effect of this bacterium, and range from its spore formation, attraction by root exudates, motility and competition in the rhizosphere to mechanisms of phosphate solubilization, siderophore production, among others. The information obtained allow a genetic exploration of these mechanisms, providing an opportunity to maximize this interaction and, in the future, favor benefits in field. Additionally, it was demonstrated the chemotaxis (attraction) potential of RZ2MS9 towards maize roots. A phylogenetic study of this PGPR, using a typing method with the pycA (pyruvate carboxylase) gene, showed that Bacillus sp. RZ2MS9 was distant from the highly monomorphic clade of B. anthracis, a human pathogen, and affiliated with B. thuringiensis (Bt) strains marketed as biopesticides for more than 60 years, suggesting the potential possibility of its safe use in the field. It is known that most, if not all, physiological activities of plants are regulated by phytormones such as the auxin IAA, which can also be synthesized by PGPRs. With more detail, genes involved in biosynthetic pathways of this phytormone were detected in the RZ2MS9 draft genome, indicating that its production occurs via the IPA (indole-3-pyruvate) pathway. In addition, plants of the dwarf tomato Micro-Tom (MT) and its mutant Δdgt, impaired in auxin sensibility, were used to specifically characterize the effects of IAA produced by Bacillus sp. RZ2MS9 in the plant growth promotion. The inoculation of RZ2MS9 caused inhibition in the primary roots growth, increase in lateral roots length and in roots total surface area of MT plants, characteristic effects of those provided by auxins. This root growth also reflected in an increase of MT plants shoot biomass. The same effects were not observed in Δdgt plants, insensitive to auxins, suggesting that the elicitation of growth promotion in MT by RZ2MS9 occurs through these phytormones. Finally, we demonstrated the effect of inoculation with Bacillus sp. RZ2MS9 on maize and soybean development and productivity under field conditions, being compared with the performance of commercial bioinoculants. In maize, the effect of bacterial inoculation was also associated with nitrogen fertilization to verify the possibility of reducing these inputs. Bacillus sp. RZ2MS9 showed significant effects on the development of both soybean (comparable to the effects of rhizobia) and maize, which, however, did not reflect a significant increase in productivity in both crops. However, the potential of this rhizobacterium is very clear because, with a cost of production of less than R$1.00 per hectare, its inoculation caused an increase of 16 sacks of maize per hectare with a 30% reduction in nitrogen fertilization, as well as an increase of 11 sacks of soybean per hectare, both compared to uninoculated control. The results presented in this study meet the great expectation of obtaining promising microbial strains aiming at more sustainable agricultural systems.
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Bhutia, Lhanjey Phuti. "Screening of phosphate solubilizing fungi from tea rhizosphere of Sikkim and formulation of bioinoculants with a plant growth promoting rhizobacterium for management of charcoal stump root disease of tea." Thesis, University of North Bengal, 2010. http://hdl.handle.net/123456789/1465.

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Owen, Darren Wayne. "Increasing agricultural grass production using novel bio-inoculants." Thesis, Bangor University, 2015. https://research.bangor.ac.uk/portal/en/theses/increasing-agricultural-grass-production-using-novel-bioinoculants(cf36a835-9554-4911-8e3c-2da4c432a579).html.

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Soil micro-organisms are a fundamental component of soil ecosystem services. Plant yields have shown to be increased through processes mediated by fungi, such as increased acquisition of important plant nutrients e.g. phosphate (P) and nitrogen (N). This has seen micro-organisms exploited commercially to create bio-inoculants (BIs). However, it is remarkably difficult to determine the effectiveness of commercially-available BIs that claim to promote crop yields as in most cases the underlying mechanisms responsible for these beneficial effects are unknown. The aims of the thesis were to examine, both within the laboratory and field, the efficacy of some commercially available BIs. Focusing on mechanisms of increased P acquisition mediated by the application of BIs. The effects of soil-P availability on BI performance were also explored. Whilst next generation DNA sequencing was utilised to explore changes in soil fungal assemblages after the introduction of BIs. As illustrated in Chapter 2, there exists much variability in efficacy testing and there is a distinct lack of robust field-based testing of commercial BIs. Chapter 3 consisted of two laboratory-based pot-trials; the first investigated the efficacy of five commercial BIs on Lolium perenne growth, the second explored the effect of an inert carrier media utilised in BI manufacture. Results found that all tested BIs increased grass yields significantly, and while many BIs contain non-living additives, treatments with living microbial fractions were found to have significantly more roots, leading to increased growth per unit P taken up by the grass. The second bioassay found the dual application of carrier media and mycorrhizal spores significantly increased grass yield, the inert carrier media a significant factor with respect to mycorrhizal root colonisation, increasing from 20% to 36%. Chapter 4 explored the phosphate solubilisation and mineralisation potential of the BI products. All BIs successfully mobilised P from recalcitrant P sources (ranging from 164 - 490 mg l-1, for inorganic-P and 0 - 39 mg l-1 for organic-P). A pot trial investigated the phosphorus efficiency ratio of Lolium perenne following application of two inorganic fertilisers of varying solubility, triple super phosphate and rock phosphate. Two of the tested BIs were found to exhibit P mediated growth gains in the form of increased yield, total shoot P, and phosphorous efficiency ratio. Yield and shoot P gains were found to be mediated by differing fractions of the living component of each BI dependent on P source. Chapters 5 and 6 were field trials exploring BI effectiveness on sites of varying P availability. Chapter 5 was on a soil of adequate P (21 mg kg-1). Overall the studies found limited benefits of BI application. Chapter 6 consisted of two field trials, one with a range of P availability (3.3 - 32 mg kg-1), the second with low P availability (8 mg kg-1). Results of the first found positive correlations with the % P and total forage P with increasing plant-available soil-P of BI-treated grass, but no significant increases in yield. The increased P acquisition and lack of yield would suggest that yield limitation was not driven by P. Similarly the results of the second trial found that while some of the BI treated grass yielded moderately higher than controls, there were no significant treatment effects on % P and total forage P, again suggesting yield gains were due to other factors. All BI treatments found to increase the N content of the forage compared to control. Chapter 7 examined the effects of various management practices on soil fungal abundances. Using DNA sequencing, fungal assemblages were found to be significantly affected by both treatment (BI application and N fertiliser), soil type and sampling date. N fertiliser was the only treatment to significantly affect fungal diversity and equitability measures. The study was able to show the potential of NGS technology, Ion Torrent™, for examining changes in fungal communities within the field. The results suggest soils with adequate levels of plant-available P may not see much benefit to warrant the application of BIs, or at least at the application rates recommended. Overall, much work is still required both within science and industry in the development and manufacture of bio-inoculants as a reliable method to increase crop yields.
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Ruíz, Pedro O., Krystel C. Rojas, and Ewald Sieverding. "La distribución geográfica de los hongos de micorriza arbuscular: una prioridad de investigación en la Amazonía peruana." Pontificia Universidad Católica del Perú. Centro de Investigación en Geografía Aplicada, 2012. http://repositorio.pucp.edu.pe/index/handle/123456789/119637.

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En los diferentes ecosistemas de la Amazonía peruana, las raíces de la gran mayoría de plantas forman una simbiosis de tipo mutualista con hongos de micorriza arbuscular (HMA). Se conoce que estos hongos constituyen un componente clave para el funcionamiento eficiente de los bosques, principalmente por dar a las plantas una mayor capacidad para absorber fósforo y agua del suelo, ayudar en la agregación de las partículas del suelo dándole mayor estabilidad y contribuir con el almacenamiento de carbono en el suelo a través de la producción de glomalina.Por otro lado, en la Amazonía del Perú existen siete tipos de suelos dominantes, de los cuales los Ultisoles ocupan aproximadamente el 65% del total, equivalentes a 49,7 millones de hectáreas, que se caracterizan por ser deficientes en fósforo. En estudios realizados en bosques en las localidades de Yurimaguas y Pucallpa, donde prevalece este tipo de suelos, se ha encontrado la presencia de HMA, asociados con la mayoría de especies vegetales nativas, lo que indica su importancia para el crecimiento y desarrollo de estas. Sin embargo, cuando se evalúan las pérdidas de la biodiversidad causadas principalmente por la deforestación se toman solo en cuenta a las especies de flora y de fauna y no se considera a la biota del suelo, incluyendo a los HMA, como si esta no tuviera relevancia en el funcionamiento de los bosques. Por otro lado, la distribución geográfica de las poblaciones de HMA es muy variable, tal como se ha descubierto en los estudios realizados que corroboran descubrimientos hechos en la Amazonía de Colombia y Brasil, de que la biota que podría estar perdiéndose por prácticas poco conservadoras de manejo y explotación. Se discuten las posibles causas de estos hallazgos y los efectos de la deforestación en las poblaciones de estos hongos y se presenta a la investigación en cuanto a la distribución geográfica de los HMA como una prioridad en la Amazonía peruana, especialmente dirigida a su utilización en programas de reforestación y recuperación de áreas degradadas con especies nativas en las diferentes áreas deforestadas de la Amazonía
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Mirzaei, Heydari Mohammad. "The role of bio-inoculants on phosphorous relations of barley." Thesis, Bangor University, 2013. https://research.bangor.ac.uk/portal/en/theses/the-role-of-bioinoculants-on-phosphorous-relations-of-barley(7fa4da0a-1d19-4df4-95ff-5da3905da1cc).html.

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Phosphorus (P) is one of the most limiting and important elements in crop production, yet often has limited availability in the soil. Manufactured inorganic P fertilisers are required to improve soil and crop P supply but their use depletes finite reserves of rock phosphate and impacts on water quality and ecosystem biodiversity. Bio-inoculants have a potential role to increase soil P supply and reduce dependence on expensive fertilisers. The objective of this thesis was to further understand the role of mycorrhizae (M) and P solubilizing bacteria (PSB) bio-inoculants and external P sources (super phosphate (SP), struvite (AMP) and rock phosphate (RP)on phosphorus availability in soil and their effects on the growth, yield and P uptake of barley. Field experiments on low P status soils in 2010 and 2011 demonstrated the potential for the use of bio-inoculants (PSB and M) in mobilizing P from soil and significantly (P <0.05) enhancing P uptake to increase growth of barley, and to a lesser extent, grain yield. It was postulated that bio-inoculant effects in the field were compromised by the presence of native M and PSB. Glasshouse pot experiments were conducted with a range of growth media: horticultural sand (zero P), field soil (low P status but with native micro-organisms) and heat sterilized field soil. These demonstrated the effects of bio-inoculants without the presence of native M and PSB, and to a lesser extent in the presence of native micro-organisms, in terms of increased plant root and shoot growth, grain yield and tissue P concentration. Across all experiments bio-inoculants (M and PSB) increased the effectiveness of water soluble SP, partially soluble AMP and insoluble RP. M and PSB were equally effective. In combination with these external P sources, bio-inoculants (M and PSB) significantly (P < 0.05) increased yield, P concentration and total P uptake, plant dry matter and concentration of P in the grain compared to P fertilizers without bio-inoculants. However, applications of P fertilizers reduced the colonization of roots by mycorrhizae. The potential role of P uptake enhancing bio-inoculants in reducing external inputs in agriculture is discussed.
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Aguilar, Rivera Katia Alejandra. "Aislamiento de bacterias solubilizadoras de fosfato,del suelo cultivado con papa (SolanumtuberosumL.)." Tesis de Licenciatura, Universidad Autónoma del Estado de México, 2020. http://hdl.handle.net/20.500.11799/109176.

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Este estudio tiene como finalidad de confirmar la capacidad solubilizadora de las cepas aisladas que se puede esperar sean de uso potencial para la formulación de un bioinoculante efectivo para el cultivo de la papa.
RESUMEN El fósforo es el segundo nutriente necesario para el desarrollo de las plantas, y de los microorganismos que habitan la rizósfera. En un modelo agrícola convencional, la mayoría de los cultivos requieren cerca de 10 a 30 kg de fósforo por hectárea que se suministran en forma de fertilizantes sintéticos, los cuales debido a sus formas no disponibles tienden a acumularse y modificar las condiciones fisicoquímicas del suelo por la inmovilización química del elemento fierro, aluminio y calcio principalmente, si bien se incrementa el rendimiento de las cosechas, no se consideran los impactos negativos que se producen, como la eutrofización de cuerpos de agua, disminución de las principales reservas de nutrientes, inmovilización de nutrientes en el suelo con los consecuentes desbalances en cultivos, aumento de los costos de producción, entre otros. Atendiendo a esta problemática surge un concepto de agricultura sustentable a través de la generación de biofertilizantes, a partir del estudio, entendimiento y aplicación de microorganismos que actúan como promotores del crecimiento vegetal o que presentan rasgos que favorecen la disponibilidad de micro y macro elementos. El presente trabajo se orientó en el aislamiento de bacterias solubilizadoras de fósforo a partir de suelo cultivado con papa (Solanum tuberosum L.) en el Estado de México. Se llevó a cabo la caracterización fisicoquímica del suelo de las zonas muestreadas, se aislaron y contabilizaron la Bacterias Heterótrofas totales, y se seleccionaron bacterias efectivas para la solubilización de fosfato mediante la siembra en medio Pikovskaya sólido, obteniendo un total de 5 cepas de las que se determinó el diámetro de halo de solubilización, siendo la cepa A3 la que presento un mayor diámetro con 19mm aproximadamente. Al confirmar la capacidad solubilizadora de las cepas aisladas se puede esperar sean de uso potencial para la formulación de un bioinoculante efectivo para el cultivo de la papa.
la clave se otorgo por parte de la SIEA de la UAEM, sin financiamiento con clave: 4635/2019SF.
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Cisneros, Moscol Jessica Elizabeth. "Aislamiento y selección de actinomicetos rizosféricos con potencial aplicación como bioinoculante en el cultivo de Solanum tuberosum sp. andigena (Papa)." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2016. https://hdl.handle.net/20.500.12672/5183.

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Evalúa la capacidad promotora de crecimiento vegetal (PGPR) de actinomicetos aislados de la rizósfera de plantaciones de papa colectadas en el distrito de San Jerónimo - Andahuaylas. Para ello, se evalúan 4 características relacionadas a las bacterias consideradas como PGPR (Solubilización de fosfatos, fijación de nitrógeno, producción de AIA y sideróforos). De las 49 cepas de actinomicetos rizosféricos aisladas y evaluadas, la cepa AND 13 presenta mejor rendimiento en las pruebas como PGPR, así como, gran capacidad de adaptación y crecimiento a diferentes temperaturas y pH; siendo identificada mediante pruebas bioquímicas y moleculares como Streptomyces sampsonii. Estos resultados indican que la cepa AND 13 podría ser utilizada a futuro como una solución alterna ante el uso excesivo de agroquímicos.
Tesis
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Tschoeke, Bruno Augusto Prohmann. "Monitoramento da interação entre rizobactéria RZ2MS16 (Burkholderia ambifaria) promotora de crescimento e bioinoculantes comerciais aplicados nas culturas de soja e milho." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-05072016-160308/.

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As culturas da soja e milho são de grande importância econômica mundial e também para o Brasil, onde a área cultivada com essas duas culturas está estimada em 45.855.900 mil hectares, distribuídas em todos estados produtores conforme suas características. A estimativa da safra mundial de soja em 2015/16 apresentou uma redução na produção global da oleaginosa para 319,0 milhões de ton, volume 1,1 milhão de ton inferior ao levantamento de dezembro de 2015. Ainda assim, trata-se de um volume recorde. Para o milho, a produção global foi de 967,9 milhões de ton, com uma redução no volume de 5,9 milhões de ton em relação ao levantamento realizado em dezembro de 2015. Nessas duas culturas são comumente utilizadas bactérias fixadoras de nitrogênio (BFN), reduzindo ou até mesmo, eliminando a aplicação de adubos nitrogenados. Estudos apontam que a simbiose entre BFN e as culturas soja e milho pode ser otimizada mediante a coinoculação com rizobatérias promotoras de crescimento de plantas (RPCP). Apesar de promissora, o estudo da utilização de BFN em associação com RPCPs é incipiente no Brasil. Assim, o presente trabalho teve como objetivo monitorar, a partir da marcação bacteriana, a interação entre a linhagem de Burkholderia ambifaria (RZ2MS16), uma rizobactéria proveniente do guaranazeiro e previamente descrita como promotora de crescimento em soja e milho e linhagens das espécies Bradyrhizobium japonicum (SEMIA5079), Bradyrhizobium diazoefficiens (SEMIA5080) e Azospirillum brasilense (Ab-v5 e Ab-v6) que são comercialmente utilizadas como bioinoculantes nessas culturas respectivamente. Os efeitos sinergisticos da interação entre RZ2MS16 e bioinoculantes comercias foram avaliados em experimento de casa de vegetação. Também foi avaliado o efeito da coinoculação de bioinculantes com outra rizobactéria proveniente do guaranazeiro, Bacillus sp. (RZ2MS9). As linhagens foram inoculadas separadamente e coinoculadas, sendo melhores resultados observados com a coinoculação das linhagens. As linhagens marcadas com genes de fluorescência selecionadas para estudo de interação foram RZ2MS16, Ab-v5 e SEMIA5080, sendo essa interação observada por microscopia de fluorescência, com também pelo reisolamento das linhagens marcadas. As linhagens RZ2MS16:pNKGFP e Ab-v5: pWM1013 e SEMIA5080:pWM1013 colonizaram todos os nichos avaliados em milho e soja, respectivamente, sendo também caracterizadas como endofíticos. Assim se observa que estudos desta natureza são de grande importância para um melhor entendimento da interação entre bactéria planta e o efeito da coinoculação no melhor desenvolvimento de plantas comercialmente utilizadas.
The soybean and corn are of great global economic importance and also to Brazil, where the area cultivated with these two crops is estimated at 45.8559 billion hectares, distributed in all producing states according to their characteristics. The estimate of the global soybean crop in 2015/16 showed a reduction in global production of oilseeds to 319.0 million tons, volume 1.1 million tons lower than the survey of December 2015. Still, it is a record volume. For corn, the total production was 967.9 million tons, with a reduction in volume of 5.9 million tons compared to the survey conducted in December 2015. In these two crops are nitrogen fixing bacteria commonly used (BFN), reducing or even eliminating the application of nitrogenous fertilizers. Studies show that the symbiosis between BFN and cultures soy and corn can be optimized by coinoculation with rhizobacteria promoting plant growth (PGPR). Although promising, the study of the use of BFN in association with RPCPs is incipient in Brazil. Thus, this study aimed to monitor, from the bacterial marking the interaction between the strain of Burkholderia ambifaria (RZ2MS16) a rhizobacteria from the guarana and previously described as a growth promoter in soybean and corn and strains of the species Bradyrhizobium japonicum (SEMIA5079), Bradyrhizobium diazoefficiens (SEMIA5080) and Azospirillum brasilense (Ab-v5 and v6-Ab) that are commercially used as inoculant these cultures respectively. The synergistic effects of the interaction between RZ2MS16 and commercial inoculant were evaluated in a greenhouse experiment. It was also evaluated the effect of coinoculation of inoculant with other rhizobacteria from the guarana, Bacillus sp. (RZ2MS9). The strains were inoculated separately and coinoculated, with best results seen with coinoculation lineages. The lines marked with fluorescence genes selected for study interactions were RZ2MS16, Ab-v5 and SEMIA5080, this interaction being observed by fluorescence microscopy with also by reisolation of the marked strains. Strains RZ2MS16: pNKGFP and Ab-v5: pWM1013 and SEMIA5080: pWM1013 colonizing all niches evaluated in corn and soybeans, respectively, also being characterized as endophytes. Thus it is observed that such studies are of great importance for a better understanding of the interaction between plant and bacteria coinoculation the effect of the improved development of plants used commercially.
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Книги з теми "Bioinoculants"

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Singh, Surender, Radha Prasanna, and Kumar Pranaw, eds. Bioinoculants: Biological Option for Mitigating global Climate Change. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-2973-3.

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M, Reddy S., ed. Bioinoculants for sustainable agriculture and forestry: Proceedings of national symposium held on Feb. 16-18, 2001. Jodhpur: Scientific Publishers (India), 2002.

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Abud, Yazmín Carreón. Hongos micorrízicos arbusculares: Conservación y bioinoculantes. Morelia, Michoacán, México: SEP, Secretaría de Educación Pública, Estados Unidos Mexicanos, 2013.

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Somani, L. L. Organic Recycling and Bioinoculants ; For Sustainable Crop Production. Agrotech Publishing Academy, 2007.

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Reddy, S. M., Ram Reddy, and S. Girisham. Bioinoculants for sustainable agriculture and forestry: Proceedings of national symposium held on Feb. 16-18, 2001. Scientific Publishers,India, 2002.

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Частини книг з теми "Bioinoculants"

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Subramanian, S. Bala, Song Yan, R. D. Tyagi, R. Y. Surampalli, and Tian C. Zhang. "Biofertilizers/Bioinoculants." In Sustainable Sludge Management, 203–30. Reston, VA: American Society of Civil Engineers, 2009. http://dx.doi.org/10.1061/9780784410516.ch09.

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Kaur, Chandandeep, G. Selvakumar, and A. N. Ganeshamurthy. "Rhizocompetence of Applied Bioinoculants." In Plant-Microbe Interactions in Agro-Ecological Perspectives, 501–11. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5813-4_25.

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Singh, Manali, Shruti Bhasin, Neha Madan, Deep Chandra Suyal, Ravindra Soni, and Dipti Singh. "Bioinoculants for Agricultural Sustainability." In Microbiological Activity for Soil and Plant Health Management, 629–41. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-2922-8_25.

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Tallapragada, Padmavathi, and Swetha Seshagiri. "Application of Bioinoculants for Sustainable Agriculture." In Probiotics and Plant Health, 473–95. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3473-2_22.

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Khan, Mujeebur Rahman, and M. Arshad Anwer. "Fungal Bioinoculants for Plant Disease Management." In Microbes and Microbial Technology, 447–88. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-7931-5_17.

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Ajilogba, Caroline Fadeke, Oluwaseyi Samuel Olanrewaju, and Olubukola Oluranti Babalola. "Application of Bioinoculants for Seed Quality Improvement." In Microorganisms for Sustainability, 265–80. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6241-4_14.

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Bottini, Rubén, Federico J. Berli, M. Victoria Salomon, and Patricia N. Piccoli. "Phytohormonal Role of Microorganisms Involved in Bioinoculants." In Microorganisms for Sustainability, 75–107. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-9570-5_5.

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Florence, Mukelabai, and Chimwamurombe Percy. "Sustainable Enhancement of Soil Fertility Using Bioinoculants." In Vermicomposting for Sustainable Food Systems in Africa, 249–60. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-8080-0_15.

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Khan, Hammad, and Nagina Parmar. "Bioinoculants: Understanding Chickpea Rhizobia in Providing Sustainable Agriculture." In Bacteria in Agrobiology: Crop Productivity, 185–215. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37241-4_8.

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Archana, Gattupalli. "Engineering Nodulation Competitiveness of Rhizobial Bioinoculants in Soils." In Microbes for Legume Improvement, 157–94. Vienna: Springer Vienna, 2010. http://dx.doi.org/10.1007/978-3-211-99753-6_8.

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Тези доповідей конференцій з теми "Bioinoculants"

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Prihastini, L., A. H. Ramelan, P. Setyono, Pranoto, and A. Supriyanto. "Isolation and Identification of Mold in Banana Bunches and Their Potential as Bioinoculants to Accelerate Decomposition of Household Organic Waste." In 10th International Seminar and 12th Congress of Indonesian Society for Microbiology (ISISM 2019). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/absr.k.210810.013.

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PANDEY, PIYUSH, SHRADHANJALI ARORA, ANCHAL SOOD, SANDEEP BISHT, and D. K. MAHESHWARI. "FORMULATION OF AN EFFECTIVE RHIZOBIUM BIOINOCULANT USING GREEN FLUORESCENT PROTEIN REPORTER SYSTEM." In Proceedings of the International Conference on CBEE 2009. WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789814295048_0093.

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Lalitha, S., and S. Nithyapriya. "Production of Bacillibactin Siderophore from Soil Bacteria, Bacillus subtilis: A Bioinoculant Enhances Plant Growth in Arachis hypogaea L. Through Elevated Uptake of Nutrients." In International Seminar on Promoting Local Resources for Sustainable Agriculture and Development (ISPLRSAD 2020). Paris, France: Atlantis Press, 2021. http://dx.doi.org/10.2991/absr.k.210609.013.

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