Дисертації з теми "Biofilm monitoring"
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Srikumar, Vivek. "Microbial biofilm monitoring by Electrochemical methods." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-302540.
Повний текст джерелаMaluleke, Moabi Rachel. "Biofilm monitoring and control using electrochemically activated water and chlorine dioxide." Diss., Pretoria : [s.n.], 2006. http://upetd.up.ac.za/thesis/available/etd-02162007-122247.
Повний текст джерелаDec, Luiza Pritsch. "Biofilm utilization for trace metal monitoring in aquatic ecosystem." reponame:Repositório Institucional da UFPR, 2016. http://hdl.handle.net/1884/43550.
Повний текст джерелаCoorientador : Prof. D.Sc. Stephan Fuchs
Dissertação (mestrado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduação em Engenharia de Recursos Hídricos e Ambiental. Defesa: Curitiba, 15/03/2016
Inclui referências : f.75-78
Resumo: Os metais traço são utilizados na indústria e na agricultura e podem estar presentes em efluentes de mineração e esgoto. Assim, esses elementos atingem o ambiente e podem ser prejudiciais aos organismos, meio ambiente e às pessoas. Um monitoramento representativo é essencial para a gestão dos recursos hídricos e consequente prevenção à poluição. Monitoramentos convencionais da água algumas vezes podem não revelar a real condição do ambiente. Isto acontece devido às condições de lançamento e chegada dos poluentes ao sistema, à tecnologia disponível para quantificar a concentração e devido às características da própria substância monitorada. No caso dos metais traço, estes têm uma atração maior por outras partículas (sólidos suspensos, solo, sedimento, carbono orgânico dissolvido), as quais sedimentam, não permanecendo na coluna d'água. Outro fator a ser considerado é que as fontes de metais traço são geralmente intermitentes e as coletas são não contínuas. Além disto, os limites de detecção são altos e não detectam tais elementos na água. Desta forma, o biofilme é uma técnica alternativa de monitoramento, pois analisa o nível de contaminação em um intervalo de tempo. Para este estudo foram construídos dois amostradores. Amostras de biofilme, água e sedimento foram coletadas ao longo de oito meses para dois pontos de monitoramento, um na bacia do rio Barigüi e outro na bacia do rio Miringuava. Parâmetros de qualidade da água, granulometria e conteúdo de metais traço foram estimados. Os resultados mostram que o biofilme identificou os metais traço em praticamente todas as campanhas, enquanto que para as amostras de água isto não aconteceu. As amostras de sedimento representaram a poluição, porém não foi possível determinar o tempo da contaminação pelo método de coleta utilizado. O biofilme representou as diferenças no uso e ocupação do solo, representando poluição consistente com cada bacia hidrográfica. Palavras-chave: biofilme, metais traço, monitoramento, rio Miringuava, rio Barigüi.
Abstract: Trace metals are used in industries and agriculture and can be present in mining and sewer effluents. In such context, these elements can enter the environment and be very harmful to organisms, environment and people. A representative monitoring is essential for water resources management and, consequently, pollution prevention. Conventional water monitoring do not always show real environment condition. That happens because of effluent release conditions, pollution arrival system conditions, available technology to identify element concentrations and monitoring substance characteristics. Specifically for trace elements, they tend to adhere to other particles (suspended matter, soil, sediment, DOC) and deposit in riverbed. Other factor is that trace metal sources are usually from intermittent discharges and collections are not continuous. Besides that, usual techniques have high quantification limit and do not identify these elements in water. Thus, biofilm is an alternative monitoring technique for trace metals evaluation since it analyses contamination level in a time space. For this study, two biofilm samplers were constructed. Biofilm, water and sediment samples were collected for an eight month period in two monitoring sites, Barigüi and Miringuava watershed. Water quality parameters, granulometry, and trace metals content were estimated. Results showed that biofilm identified metals in almost every campaign, while water samples did not. On the other hand, sediment samples represented pollution but it was not possible to determine the contamination time by the used collection method. Biofilm also represented differences in soil use and occupation, representing consistent pollution potential for each basin. Keywords: biofilm, trace metals, monitoring, Miringuava River, Barigüi River.
Roßteuscher, Tobias. "Online monitoring of biofilm in microchannels with thermal lens microscopy." kostenfrei, 2009. http://mediatum2.ub.tum.de/node?id=734672.
Повний текст джерелаEvans, Emily Amaya. "Ultrasonic reflectometry for monitoring biofilm growth on water treatment membranes." Diss., Connect to online resource, 2005. http://wwwlib.umi.com/cr/colorado/fullcit?p1427775.
Повний текст джерелаYang, Jingjing. "Controlling and monitoring of deammonification process in moving bed biofilm reactor." Licentiate thesis, KTH, VA-teknik, Vatten, Avlopp och Avfall, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-98624.
Повний текст джерелаPartiell nitrifikation i kombination med anammoxprocess, som kallas för deammonifikationprocess, anses vara mer miljövänlig jämfört med konventionell nitrifikation/denitrifikation pga minskat energibehov samt låga utsläpp av CO2 och N2O. Löst syre (DO) är en viktig parameter som påverkar hastigheten för kväverening och aktiviteten hos olika mikroorganismer. DO koncentrationer bör vara på en viss nivå för att ammoniumoxiderande bakterier (AOB) skall producera en tillräcklig mängd NO2-N för anammoxreaktionen, men inte heller för hög då hög NO2-N nivå ger en anammoxhämmande effekt eller ökad tillväxt av nitritoxiderande bakterier (NOB). I denna studie har undersökningar utförts både i laboratorie- och pilotskala för att utvärdera inverkan av olika luftningsstrategier, (som kännetecknas av koncentrationen av löst syre - DO och förhållandet (R) mellan tider för icke luftade och luftade faser), på deammonifikationprocessen i en MBBR (Moving Bed Biofilm Reactor). Tre serier av satsvisa försök utfördes i laboratorieskala med olika syre koncentrationer (2, 3, 4 mg/l) och R värden (0 - kontinuerlig luftning; 1/3, 1, 3 - intermittent luftning), men med samma initiala ammonium-koncentration, volym av den rörliga bädden och temperatur. Man fann att effekten av löst syre (DO) på deammonifikationen var beroende på R-värde. Vid R = 0 och R = 1/3, gav en ökning av löst syre (DO) en signifikant ökning i kvävereningshastigheten, medan för R = 1 och R = 3 observerades samma hastighet i processen oberoende av löst syrehalt (DO). Den högsta hastigheten för kväveavskiljning 3,33 gN/m2.d (avskiljningsgraden var lika med 69,5%) erhölls vid R=1/3 och DO=4 mg/l. Betydligt lägre värden (från 1,17 till 1,58 gN/m2.d) observerades vid R=1 och R=3 för varje undersökt halt av löst syre (DO). Det var en följd av minskad varaktighet av luftad fas och mindre mängd av kvarvarande nitrit i icke luftade faser jämfört med R= 1/3. Pilotskaleförsök utfördes i en MBBR med en arbetsvolym på 200 L. Pilotanläggningen har drivits i 1,5 år med att avlägsna kväve från rejektvatten från avvattning av rötslam. Aktiviteten hos olika grupper av mikroorganismer i biofilmen mättes genom tester av specifik anammoxaktivitet (SAA), syreupptagningshastighet (OUR) och nitratutnyttjandegrad (NUR). Driften var uppdelat i 7 perioder med olika kvävebelastning och luftningsstrategier. Den högsta hastigheten och grad av kväveavskiljning erhölls då DO var 3,5 mg/l och R uppgick till 1/3. Aktivitetstester visade att anammoxbakterier och AOB spelade dominerande roller i biofilmen. De genomsnittliga och maximala värden för specifika anammoxaktiviteten (SAA) var 3,01 gO2/m2.d och 4,3 gO2/m2.d, respektive. 4,0 gO2/m2.d som medelvärde och högsta värde på 5,1 gO2/m2.d erhölls för syreupptagningen för AOB aktivitetstester. Studien visade att användning av en lämpligt vald luftningsstrategi minskar energiförbrukningen utan några negativa effekter på processen. Införande av anaeroba faser och hög kvävetillförseln ökar aktiviteten för anammoxbakterier och NOB-aktiviteten begränsades.
QC 20120628
Mariana, Frida. "Chip-Calorimetric Monitoring and Biothermodynamic Analysis of Biofilm Growth and Interactions with Chemical and Biological Agents." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-191577.
Повний текст джерелаEstrada, Leypón Oscar Emilio. "Micro-Nano-Bio Systems for on-line monitoring of in vitro biofilm responses." Doctoral thesis, Universitat Ramon Llull, 2015. http://hdl.handle.net/10803/300595.
Повний текст джерелаEl trabajo presentado en esta tesis doctoral tiene como principal objetivo la contribución en el campo de la microbiología para entender los biofilms y el posible control de desarrollo mediante el uso de métodos y enfoque multidisciplinar. Los biofilms están definidos como comunidades de microorganismos que crecen embebidos en una matriz exopolisacárida y se adhieren a una superficie inerte o tejido vivo. La formación de los biofilms bacterianos tiene un gran interés en microbiología clínica debido al desarrollo de infecciones que son causadas por contacto directo o por colonización de dispositivos médicos implantados y prótesis. Actualmente se consideran la causa de más del 60 % de las infecciones bacterianas. El problema de los biofilms bacterianos a nivel clínico es que muestran mejor resistencia a antibióticos llegando incluso a ser de 500 a 5000 veces más resistentes a agentes antimicrobianos comparado a la misma bacteria planctónica (bacteria en suspensión). Ha habido muchas tentativas de adaptar métodos a laboratorios clínicos donde se reproducen las condiciones para el desarrollo de biofilms, pero aún no se ha llegado a obtener óptimos protocolos estándar para este propósito de monitorizar la formación y toxicidad en tiempo real. Ha crecido el interés en diseño, desarrollo y utilización de dispositivos de microfluídica que puedan emular los fenómenos biológicos que ocurren con diferentes geometrías, dinámica de fluidos y restricciones de transporte de biomasa en microambientes fisiológicos. La investigación descrita en esta tesis se lleva a cabo con diferentes métodos “label-free” basados en variación acústica y/o propiedades eléctricas para la monitorización de biofilms. El trabajo presentado en esta monografía describe un dispositivo “custom-made” para la utilización de Espectroscopia de impedancia electroquímica como herramienta útil para obtener información de adherencia y formación de biofilms. El hecho de añadir nanopartículas como segundo biosensor permite la correlación de biofilm con su toxicidad en tiempo real para la detección del punto óptimo del tratamiento de biofilms. Finalmente el diseño de esta tecnología es usada para el ensayo de la respuesta de biofilms a antibióticos como modelo in vitro de infecciones causadas por biofilms.
The work presented in this thesis has the main aim to contribute in the field of clinical microbiology to understand the biofilms and the possible of development through the use of methods with multidisciplinary approach. Biofilms are defined as communities of microorganisms that grow embedded in a matrix of exopolysaccharides and adhering to an inert surface or living tissue. The formation of bacterial biofilms has an interest in clinical microbiology with the development of infections that usually arise from either direct contact or the colonization of implanted medical devices and prostheses. Currently they are considered the cause of over 60% of bacterial infections. The problem of bacterial biofilms at clinical level is showing great resistance to antibiotics, so that the biofilm bacteria are 500 to 5000 times more resistant to antimicrobial agents that the same bacteria grown in planktonic cultures (bacteria in suspension). There have been attempts to adapt methods to clinical laboratories where they reproduce the conditions of biofilms, but have not yet adopted an optimal standard protocol for this purpose to follow-up the formation and toxicity in real-time. There has been a growing interest in design, development and utilization of microfluidic devices that can emulate biological phenomena that occur in different geometries, fluid dynamics and mass transport restrictions in physiological microenvironments. The research described in this thesis deals with different label-free methods based on variation of acoustic and electric properties for biofilm monitoring. The work presented in this monograph describe a custom-made device for using electrochemical impedance spectroscopy (EIS) as useful tool to obtain information of adherence and formation of biofilms. The addition of nanoparticles as toxicity biomarker allows the correlation of biofilm formation with its toxicity in real-time for detention of the optimal point for biofilm treatment. Finally the design of this technology is used for testing the biofilm response to antibiotic as in vitro model of biofilm-related infection.
TUCCI, MATTEO. "MICROBIAL ELECTROCHEMICAL SENSORS FOR FRESHWATER AND WASTEWATER MONITORING." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/702269.
Повний текст джерелаBrain, Stephen. "Monitoring microbial biofilms." Thesis, London South Bank University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337401.
Повний текст джерелаMariana, Frida [Verfasser], Thomas [Akademischer Betreuer] Bley, Thomas [Akademischer Betreuer] Maskow, and Ulrich [Akademischer Betreuer] Szewzyk. "Chip-Calorimetric Monitoring and Biothermodynamic Analysis of Biofilm Growth and Interactions with Chemical and Biological Agents / Frida Mariana. Betreuer: Thomas Bley ; Thomas Maskow. Gutachter: Thomas Bley ; Thomas Maskow ; Ulrich Szewzyk." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://d-nb.info/1083463063/34.
Повний текст джерелаMariana, Frida Verfasser], Thomas [Akademischer Betreuer] [Bley, Thomas [Akademischer Betreuer] Maskow, and Ulrich [Akademischer Betreuer] Szewzyk. "Chip-Calorimetric Monitoring and Biothermodynamic Analysis of Biofilm Growth and Interactions with Chemical and Biological Agents / Frida Mariana. Betreuer: Thomas Bley ; Thomas Maskow. Gutachter: Thomas Bley ; Thomas Maskow ; Ulrich Szewzyk." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://d-nb.info/1083463063/34.
Повний текст джерелаMoore, Zoe Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Molecular toolbox for the monitoring and control of water distribution biofilms." Awarded By:University of New South Wales. Biotechnology & Biomolecular Sciences, 2010. http://handle.unsw.edu.au/1959.4/44666.
Повний текст джерелаSchofield, Amy Louisa. "Novel Biophysical Techniques for Monitoring the Development and Stability of Microbial Biofilms." Thesis, University of Liverpool, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490711.
Повний текст джерелаMaal-Bared, Rasha. "Comparing the distribution of pathogenic bacteria and common indicator microorganisms in biofilms on different surface types in an agricultural watershed in British Columbia (Canada)." Thesis, University of British Columbia, 2008. http://hdl.handle.net/2429/856.
Повний текст джерелаGonzales, Fernanda Pereira [Verfasser], and Otto S. [Akademischer Betreuer] Wolfbeis. "Photodynamic inactivation of microbial biofilms: impact of Hsp70 expression and non-invasive optical monitoring of oxygen during photodynamic inactivation / Fernanda Pereira Gonzales. Betreuer: Otto S. Wolfbeis." Regensburg : Universitätsbibliothek Regensburg, 2013. http://d-nb.info/1038092000/34.
Повний текст джерелаUseh, Kowho Pearl. "Monitoring biostability and biofilm formation potential in drinking water distribution systems." Thesis, 2017. https://hdl.handle.net/10539/24189.
Повний текст джерелаThe foremost aim of potable water treatment is to produce water that does not pose a health risk when consumed and/or otherwise used. Nevertheless, research has established that the quality of treated water deteriorates during distribution. The nature and extent of this deterioration varies from system to system and from time to time. The aim of this research study was to monitor the parameters that are known to significantly affect biostability and biofilm formation potential in drinking water distribution systems. Biweekly water samples were collected from thirteen sites, across a section of Johannesburg Water’s network, between September 2015 and August 2016. All samples were assayed for a suite of fifteen water quality parameters using standard methods. Heightened temperature, dearth of chlorine residuals, availability of biodegradable dissolved organic carbon (BDOC), and advanced water age all engendered the loss of biostability (instability). Biostability controlling parameters varied seasonally and spatially. Samples collected during spring and summer, in general, were most likely to be characterized by instability than samples collected during winter and autumn. Samples collected from sites RW80, RW81, RW82, RW83, RW104 and RW253 were more prone to instability compared to samples from other sites. From the results, it is clear that chlorine residuals ought to be kept above 0.2 mg/l, and, BDOC below 0.3mg/l to prevent the loss of heterotrophic stability in distributed water. BDOC concentrations can be decreased by, flushing the pipes, cleaning reservoirs regularly and by further treating feed water before distributing. Booster disinfection can be relied upon to ensure that chlorine residuals are maintained throughout the network. Apart from potential health risks, biological instability and biofilm growth can result in non-compliance with regulations.
MT2018
Roßteuscher, Tobias [Verfasser]. "Online monitoring of biofilm in microchannels with thermal lens microscopy / Tobias Roßteuscher." 2009. http://d-nb.info/995182728/34.
Повний текст джерелаMariana, Frida. "Chip-Calorimetric Monitoring and Biothermodynamic Analysis of Biofilm Growth and Interactions with Chemical and Biological Agents." Doctoral thesis, 2014. https://tud.qucosa.de/id/qucosa%3A29133.
Повний текст джерелаOlugbuo, Zita. "Investigation of the efficacy of BDOC protocols used in biofilm measurement and monitoring." Thesis, 2017. https://hdl.handle.net/10539/25147.
Повний текст джерелаAccess to good quality drinking water is essential for the maintenance of public health. To guarantee a steady supply of good quality water, water treatments plants are designed to provide potable water that meets national and, where necessary, local water quality standards. While the protection of natural water resources against pollution, and proper treatment of water at treatment plants are both crucial to the provision of safe drinking water, the reality is that the quality of treated water can degrade during distribution. Microbial proliferation within distribution systems can cause problems such as unpleasant tastes and odours as well as the proliferation of pathogenic microorganisms. For most utilities, it is biofilm that grows on pipe surfaces that act as permanent inocula continuously inoculating the bulk water as it flows through the distribution system. Distribution system biofilm growth and the resulting impact on water quality can be minimized by various treatment processes, designed to remove biodegradable organic matter (BOM) from the water. The removal of BOM is of great importance to water utilities because it eliminates bacterial regrowth and the many associated water quality issues. Hence, the spatial and temporal mapping of biodegradable organic carbon (BDOC) offers water utilities an effective strategy in managing the BOM in the distribution system. This research is aimed at evaluating the applicability of BOM measurement protocols to monitoring biostability and biofilm formation potential within a drinking water distribution system (DWDS). This study specifically investigated the efficacy of a simplified version of the high-density BDOC test as a protocol for monitoring BDOC in finished water. The high-density BDOC protocol was found to be a more streamlined approach in contrast to the assimilable organic carbon (AOC), and provides a suitable monitoring mechanism for lowering biofilm formation potential in DWDSs.
CK2018
Zheng, Yi-Ting, and 鄭伊庭. "In Situ Monitoring of Rhodococcus sp. SD-74 Biofilm Development by Raman Microspectroscopy and Imaging." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/97677436447423716613.
Повний текст джерела國立交通大學
應用化學系碩博士班
100
Most bacteria in natural habitats live as biofilms, in which bacterial cells are embedded in matrices of extracellular polymeric substances adherent to a surface. Recent studies have revealed that the biofilm mode of living increases the survival rate of bacteria by e.g., acquiring extremely high antibiotic resistance. Although unique structures and functions of biofilms have attracted much attention in many disciplines of science, it remains challenging to study biofilms in vivo and in situ as well as with high chemical specificity. In the present study, we focus on the biofilms of the bacterial species of practical interest known as Rhodococcus sp. SD-74 and monitor the process of its biofilm development using Raman microspectroscopy and imaging. Because Raman spectroscopy is nondestructive and label-free, and is less hampered by the presence of water, Raman microspectroscopy and imaging are an ideal tool to study biofilms in situ. We successfully obtained detailed distribution and composition changes within R. sp. SD-74 biofilm during the development process. We found that the concentration of carotenoids in the biofilm matrix drastically increases as biofilm development proceeds. To interpret this phenomenon of carotenoid accumulation, we hypothesize that carotenoids act as an antioxidant in R. sp. SD-74 biofilms and counter possible oxidative stress. Raman imaging experiments performed under different light-illumination conditions support this hypothesis.
BÁRTOVÁ, Marie. "Detekce a monitoring potenciálně toxických sinicových lipopeptidů." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-394171.
Повний текст джерелаChen, Jun-Yuan, and 陳俊原. "Investigation of the Streptococcus mutans detection specificity and the real-time monitoring dental-biofilm formation of purple membrane-based photoelectric biochips." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/10306225571295551522.
Повний текст джерела國立臺灣科技大學
化學工程系
105
Bacteriorhodopsin (BR) is a retinal protein residing in Halobacterium salinarum purple membrane (PM). When illuminated, BR pumps a proton gradient across PM, leading to photocurrent generation. To improve the selectivity of antibody-PM and aptamer-PM composite sensor chips for Streptococcus mutans detection, which were previously developed by using the principles that PM photocurrent correlates linearly with illumination intensity and that bacteria scatter light, this study used glycine to block the residual active moiety of the homobifunctional crosslinker fabricated on PM-coated chips for recognition-element conjugation. For the detection of 106 CFU/mL bacteria, without prior glycine blocking, the antibody-PM chips exhibited not only a 53 % photocurrent reduction on S. mutans detection, but also 21% and 24% reductions for E. coli and L. acidophilus, respectively. On the other hand, with prior glycine blocking, the photocurrent reductions of the chips decreased to 8% and 7% on E. coli and L. acidophilus detections, respectively, indicating selectivity improvement of the antibody-PM chips. The effect of glycine blocking was less pronounced with the aptamer-PM chips because the photocurrent reductions decreased from 14% and 19% to only 12% and 15% for E. coli and L. acidophilus, respectively. Furthermore, a double-decked microfluidics device was designed and constructed to real-time monitor the formation of S. mutans biofilm on the substrate placed in the upper deck with a PM-coated photoelectric chip mounted in the lower deck. A diluted growth medium and an electrolyte buffer were injected into the upper and lower decks, respectively. The comparison of ITO glass modified with self-assembled monolayers with different terminal groups suggested that the hydrophobic and positively charged surface most easily caused S. mutans to form a mature 3D-strucutred biofilm. Moreover, the anti-biofilm peptides coated on the amine-terminated substrate effectively inhibited biofilm formation, with the inhibition efficiency increasing with their coating amounts. Therefore, the results confirmed the feasibility of real-time monitoring biofilm formation with the developed double-decked microfluidics.
Cicotti, Flavia. "Food chains Hygiene: monitoring of retail products and characterization of isolated microbial species (virulence factors, ability to form biofilm, antibiotic resistance)." Tesi di dottorato, 2013. http://www.fedoa.unina.it/9133/1/Cicotti_Flavia_25.pdf.
Повний текст джерелаHo, Chi-hua, and 何齊華. "Monitoring the Quorum Sensing Gene Expression within Pseudomonas aeruginosa biofilms Using Green Fluorescent Protein." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/01951349637826692264.
Повний текст джерела國立臺灣大學
農業化學研究所
90
Pseudomonas aeruginosa is an opportunistic pathogen that can cause serious infections in immunocompromised hosts. When forming biofilms, P. aeruginosa will increase the resistence to antimicrobial agents. In this study, we have constructed the quorum-sensing systems, lasI and rhlI, recomplementary plasmids to examine the contribution of these two genes to the biofilm development. After 72 hours of cultivation, the cell densities of the PAO1(wild type), PDO100(ΔrhlI) and JP2(ΔlasIΔrhlI) biofilm reached 5.0±0.3x109 CFU/cm2, 6.4±3.9x108 CFU/cm2 and 1.6±1.1x108 CFU/cm2 respectively. The rhlI recomplementary to PDO100 biofilm reached 1.5±0.6x109 CFU/cm2 while lasI recomplementary to JP2 was 1.9±0.6x108 CFU/cm2. The polysaccharide and protein ratios of PAO1(wild type), PDO100(ΔrhlI) and JP2(ΔlasIΔrhlI) biofilm reached 0.8±0.1, 0.7±0.1, and 0.4±0.2 respectively. The rhlI recomplementary to PDO100 biofilm reached 0.8±0.2 while lasI recomplementary to JP2 was 0.7±0.2. Combined the above data with in conjuction of cryoembedding and cryosectioning couple fluorescent staining results, the lasI gene seems to play an important role in polysaccharide production while rhlI gene associated with the cell density in biofilms. To further elucidate the quorum-sensing gene expression within biofilms, we utilized green fluorescent protein to be the reporter gene to directly visulize the gene expression. The result indicated that the quorum-sensing gene expressed instinctly around biofilm.
Stensland, Michael F. "The benthic macroinvertebrate survey of Butternut Creek, Otsego County, New York /." 2005. http://www.oneonta.edu/academics/biofld/PUBS/OP/Real%20Stensland%20OP39.pdf.
Повний текст джерелаDHAHRI, Samia. "Analyse topographique, mécanique et électrochimique à l'échelle sub-micrométrique de processus pilotés par les bactéries." Phd thesis, 2013. http://tel.archives-ouvertes.fr/tel-01044714.
Повний текст джерела