Дисертації з теми "Bioanalytical applications"
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Driscoll, Peter F. "Bioanalytical Applications of Chemically Modified Surfaces." Digital WPI, 2009. https://digitalcommons.wpi.edu/etd-dissertations/465.
Повний текст джерелаAnazia, Oge. "Chemistry of Zirconia and Its Bioanalytical Applications." TopSCHOLAR®, 2009. http://digitalcommons.wku.edu/theses/127.
Повний текст джерелаPereiro, Iago. "Microfluidic magnetic fluidized bed for bioanalytical applications." Thesis, Paris 6, 2016. http://www.theses.fr/2016PA066089/document.
Повний текст джерелаWith the use of an external magnetic field and magnetic microbeads, the microfluidic magnetic fluidized bed system enables fluidization phenomena at the microscale. This results in flow-through operations at low driving pressures with intimate liquid/solid contact and a continuous beads recirculation, interesting for efficient biological target preconcentration applications. The physical system has been characterized, showing the importance of chamber angle of aperture and height confinement as well as magnetic field distribution parameters, to obtain fluidization and further enhance mixing and maximize beads density. Further, the potential of the fluidized bed as a platform for analytical bioassays has been successfully explored with a series of biologically relevant applications: (1) the preconcentration of rare Alzheimer’s biomarkers together with their in situ fluorescence labeling for future enhanced detection with hyphenated techniques; (2) the label-free sensitive detection of bacteria in liquid food samples through the specific immunocapture and on-chip culture of these microorganisms and the resulting physical changes induced in the fluidized support; (3) the gene-specific extraction of DNA and its subsequent enzymatic amplification on the surface of the beads, coupled to a microarray detection system for a multiplexed detection of cancer-inducing mutations. These results show that the applications of the magnetic fluidized bed go beyond its initial conception as a dynamical affinity-based concentrator, serving as an efficient platform for molecular biology protocols and even making use of its inherent auto-regulating properties as a detection mechanism
Selegård, Robert. "Polypeptide functionalized gold nanoparticles for bioanalytical applications." Doctoral thesis, Linköpings universitet, Molekylär fysik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-106719.
Повний текст джерелаCannan, Susan. "Microelectrode methods for bioanalytical and biophysical applications." Thesis, University of Warwick, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397013.
Повний текст джерелаDolatmoradi, Ata. "Thermally-Assisted Acoustofluidic Separation for Bioanalytical Applications." FIU Digital Commons, 2017. http://digitalcommons.fiu.edu/etd/3371.
Повний текст джерелаGallagher, Jane. "Protein nanoparticle conjugates for use in bioanalytical applications." Thesis, University of Strathclyde, 2011. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=17065.
Повний текст джерелаSentic, Milica. "Electrogenerated chemiluminescence : from mechanistic insights to bioanalytical applications." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0307/document.
Повний текст джерелаElectrogenerated chemiluminescence (ECL) is a powerful analytical technique exploited for clinical, industrial and research applications. The high sensitivity and good selectivity, makes ECL a tool-of-choice analytical method for a broad range of assays, most importantly for a large number of commercialized bead-based immunoassays. In the present thesis, we aimed to study the ECL phenomenon and its application in development of new analytical methods.In the first part of this work, we used an imaging technique to investigate the ECL mechanisms operating in bead-based assays. Spatial reactivity mapping at the level of a single functionalised bead provides a new strategy to test the co-reactant efficiency and shows associated optical focusing effects.In the second part, the design of a novel anti-transglutaminase ECL immunoassay for celiac disease diagnostic is shown using nanoelectrode ensembles as bioelectroanalytical platforms. We also studied the characteristics of ECL generated by arrays of boron-doped-diamond nanoelectrodes (BDD NEAs) as a promising materials for bioapplications. The ECL efficiency of two co-reactants at BDD NEAs was investigated.Finally, bipolar electrochemistry is a ‘‘wireless’’ process that was exploited for the controlled motion of conductive objects exposed to an electric field in the absence of direct ohmic contact. In the third part of the thesis, we report ECL coupled to bipolar electrochemistry for tracking the autonomous trajectories of swimmers by light emission. We further expanded this concept for dynamic enzymatic sensing of glucose concentration gradient using ECL light emission as an analytical readout
Gruenhagen, Jason Alan. "Bioanalytical Applications of Real-Time ATP Imaging Via Bioluminescence." Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2003. http://www.osti.gov/servlets/purl/822057-FTilZ3/native/.
Повний текст джерелаPublished through the Information Bridge: DOE Scientific and Technical Information. "IS-T 2604" Jason Alan Gruenhagen. 12/12/2003. Report is also available in paper and microfiche from NTIS.
Wimalasena, Rohan Lalith. "Preparation and characterization of immunochemical reagents for bioanalytical applications." Diss., The University of Arizona, 1991. http://hdl.handle.net/10150/185457.
Повний текст джерелаJohannesson, Nina. "Column Development in Capillary Electrophoresis and Electrochromatography for Bioanalytical Applications." Doctoral thesis, Uppsala universitet, Analytisk kemi, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7124.
Повний текст джерелаWicks, Arriel. "Luminescent Quantum Dot and Protein Composite Nanoparticles for Bioanalytical Applications." ScholarWorks@UNO, 2010. http://scholarworks.uno.edu/td/1149.
Повний текст джерелаQamar, Ahmad Zaman. "DEVELOPING WAX-ON-PLASTIC PLATFORMS FOR BIOANALYTICAL AND BIOMEDICAL APPLICATIONS." OpenSIUC, 2019. https://opensiuc.lib.siu.edu/dissertations/1759.
Повний текст джерелаCai, Xiaohan. "¿¿¿¿¿¿Development of Bioanalytical Methods for Clinical Applications and Drug Screening." Cleveland State University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=csu1314982525.
Повний текст джерелаKumar, Suresh. "Design, Fabrication, and Optimization of Miniaturized Devices for Bioanalytical Applications." BYU ScholarsArchive, 2015. https://scholarsarchive.byu.edu/etd/5979.
Повний текст джерелаKim, Youngmi. "Molecular engineering of multifunctional nucleic acid probes for bioanalytical and biomedical applications." [Gainesville, Fla.] : University of Florida, 2008. http://purl.fcla.edu/fcla/etd/UFE0023647.
Повний текст джерелаKim, Junseok. "Bioanalytical Applications of Intramolecular H-Complexes of Near Infrared Bis(Heptamethine Cyanine) Dyes." Digital Archive @ GSU, 2008. http://digitalarchive.gsu.edu/chemistry_diss/23.
Повний текст джерелаMezzanotte, Laura <1982>. "Bioanalytical applications of multicolour bioluminescence imaging: new tools for drug discovery and development." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2011. http://amsdottorato.unibo.it/3536/.
Повний текст джерелаScott, Daniel F. "DEVELOPMENT OF LUMINESCENT SENSING SYSTEMS WITH CLINICAL APPLICATIONS." UKnowledge, 2011. http://uknowledge.uky.edu/gradschool_diss/184.
Повний текст джерелаChapman, Gala M. "Spectroscopic Studies of Carbocyanine and 2,4,6- Trisubstituted Pyridine Dyes for Bioanalytical and pH Indicating Applications." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/chemistry_theses/47.
Повний текст джерелаKnittle, James Eugene. "Bioanalytical and photophysical applications of sensitive nonlinear wave-mixing spectroscopy based on laser-induced gratings /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2004. http://wwwlib.umi.com/cr/ucsd/fullcit?p3138952.
Повний текст джерелаDebela, Mehdi Ahmed. "Bioanalytical applications of polyoxometalates using molecular tools for detection of DNA and SNPs in primer extension." Doctoral thesis, Universitat Rovira i Virgili, 2014. http://hdl.handle.net/10803/277386.
Повний текст джерелаEl objetivo general de la presente tesis es el desarrollo de una plataforma para la detección electroquímica de mutaciones genéticas empleando un método de alta especificidad basado en la extensión de secuencias de ADN, llamadas cebadores, dispuestas organizadamente sobre una superficie (APEX). Por tanto el trabajo incluye la preparación de moléculas que constituyen marcadores electroquímicos y que fueron posteriormente enlazadas de forma covalente a las cuatro bases de ADN y, además, el desarrollo de una plataforma robusta resistente a altas temperaturas, que son necesarias durante la incorporación por la via enzimática de las bases de ADN marcadas. En la primera parte del presente trabajo, se sintetizaron dos clases de polioxometalatos (llamados Keggin and Dawson POM) y fueron covalentemente enlazados a secuencias de ADN. Además, junto a otros marcadores electroquímicos, fueron enlazados covalentemente a bases de ADN individuales para la detección de mutaciones genéticas. En la segunda parte se desarrolló un nuevo método para la funcionalización de superficies de carbón vítreo basado en procesos electroquímicos consecutivos de hidrogenación y cloración. Estas superficies modificadas fueron utilizadas para la inmovilización de moléculas tioladas, incluyendo ADN tiolado. Finalmente, las moléculas sintetizadas y las superficies modificadas fueron usadas para la detección electroquímica de mutaciones genéticas siguiendo la metodología APEX.
This PhD work was started with the intention of developing platform for electrochemical detection of mutations using highly specific method called arrayed primer extension. So the work involves the preparation of labels to be linked with the four different DNA bases and the preparation of robust platform which can withstand the high temperature during enzymatic incorporation of the labelled DNA bases. The first part of this work deals of the preparation of compounds called polyoxometalates (Keggin and Dawson types) and their covalent attachment to DNA sequence and also, to DNA single bases along with other organic redox labels for mutation detection. In the second part while trying to achieve a thermally stable surface tethered DNA probes, a new method of surface functionalisation based on electrochemical hydrogenation followed by electrochemical chlorination of glassy carbon was developed. The so prepared surfaces were used for immobilisation of thiolated molecules including thiolated DNA. Finally the synthesised molecules and modified surfaces were used for electrochemical detection of genetic mutation following the APEX protocol.
Atherton, Adrian Ashley. "Nonlinear wave-mixing spectroscopic methods for bioanalytical and biophysical applications with sensitive detection at the single cell level." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3230037.
Повний текст джерелаTitle from first page of PDF file (viewed November 17, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references.
Pan, Tao. "Towards early stage disease detection in microdevices : fabrication and testing of micro total analysis systems for bioanalytical applications / /." Diss., CLICK HERE for online access, 2007. http://contentdm.lib.byu.edu/ETD/image/etd1836.pdf.
Повний текст джерелаPan, Tao. "Towards Early State Disease Detection in Microdevices: Fabrication and Testing of Micro Total Analysis Systems for Bioanalytical Applications." BYU ScholarsArchive, 2007. https://scholarsarchive.byu.edu/etd/1351.
Повний текст джерелаGamez, Gerardo. "Advances in analytical spectrochemistry with ionized gases. I. Improved fundamental understanding through laser based techniques. II. Novel bioanalytical applications." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3223049.
Повний текст джерела"Title from dissertation home page (viewed June 28, 2007)." Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 3105. Adviser: Gary M. Hieftje.
Tanase, Otilia Maya <1982>. "Development and applications of hollow fiber flow field-flow fractionation in the bioanalytical field. Studies of aggregation phenomena in complex protein samples." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amsdottorato.unibo.it/6277/.
Повний текст джерелаSouza, Glalci Alves de. "Estudo mecanístico do sistema peroxioxalato com diferentes catalisadores." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-20092017-104722/.
Повний текст джерелаThe peroxyoxalate reaction is the non-enzymatic chemiluminescence system with the highest efficiency, achieving quantum yields of up to 50%. The chemiluminescence of this system has been widely used in analytical and bionanalytical applications in order to detect analytes of biological and medicinal interests. The present work consisted in a study of the peroxyoxalate reaction with different catalysts and the determination of its mechanism in experimental conditions different from those studied before, aiming its adaptation to aqueous media for future applications. The use of sodium salicylate as base catalyst for this system in pure organic medium, in substitution to imidazole, was studied and shown that salicylate acts as a general and/or specific base catalyst. The maximum quantum yield obtained for the transformation in these conditions was in the order of 10-3 E mol-1. Emission experiments were also performed in a binary solvent system composed of 1,2- dimethoxyethane/aqueous phosphate buffer at different pH values as catalyst, using oxalic esters with different reactivities. In these conditions the occurrence of general acid catalysis and general base catalysis was observed, since the rate constants proved to be dependent on the buffer concentration. In addition, sodium salicylate was also used as catalyst in the binary 1,2-dimethoxyethane/water system with different oxalic esters, indicating its important catalytic role in the transformation. These studies allowed it to establish a reproducible peroxyoxalate system in partially aqueous media using sodium salicylate as base catalyst, which may increase the use of the chemiluminescence of this system in analytical and bioanalytical applications. However, when the experiments were performed in a medium containing mostly water, salicylate did not act as an efficient catalyst in these conditions. Even in the absence of catalyst, the reaction proved to be reproducible in the medium containing mostly water as solvent
Rainville, Paul. "The investigation and application of alternative strategies for LC/MS based bioanalytical research." Thesis, King's College London (University of London), 2013. https://kclpure.kcl.ac.uk/portal/en/theses/the-investigation-and-application-of-alternative-strategies-for-lcms-based-bioanalytical-research(9e73e438-c941-4e4f-8502-69f6e4decef9).html.
Повний текст джерелаRömsing, Susanne. "Development and Validation of Bioanalytical Methods : Application to Melatonin and Selected Anti-Infective Drugs." Doctoral thesis, Uppsala universitet, Analytisk kemi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-131519.
Повний текст джерелаFelaktigt tryckt som Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 703
Öhman, Daniel. "Bioanalytical development for application in therapeutic drug monitoring : focus on drugs used in psychiatry /." Linköping : Univ, 2003. http://www.bibl.liu.se/liupubl/disp/disp2003/med775s.pdf.
Повний текст джерелаMak, Wing Cheung. "The applications of layer-by-layer technology in bioengineering and bioanalytics /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202004%20MAK.
Повний текст джерелаBenabou, Zdaou Sanae. "Application of analytical and chemometric methodologies to study complex bioanalytical processes involving DNA i-motif structures." Doctoral thesis, Universitat de Barcelona, 2018. http://hdl.handle.net/10803/663796.
Повний текст джерелаLa estructura de l’ADN coneguda com “i-motif” es forma en seqüències riques en bases citosina (C). L’esquelet de l’i-motif està format per parells de bases C·C+ intercalats i estabilitzats per ponts d’hidrogen. S'ha demostrat la formació in vitro d'aquesta estructura en seqüències d'ADN corresponents a les regions promotores de diversos oncògens, com el c-kit, el c-myc o el bcl-2. Recentment, s'ha demostrat la primera evidència de la seva presència in vivo. La present Tesi Doctoral tracta de l'aplicació de metodologies analítiques i quimiomètriques per estudiar processos bioanalítics complexos que en els que intervenen aquestes estructures. Les seqüències estudiades corresponen a les regions promotores dels gens nmyc i SMARCA4. D'una banda, s'ha estudiat l'estabilitat de les estructures formades per aquestes seqüències segons variacions de pH, temperatura, força iònica o presència de lligands en condicions d'estat estacionari. D'altra banda, s'ha avaluat el potencial d'espectroscòpia ultraràpida per a l'estudi de processos cinètics ràpids provocats per la llum. Al llarg de la Tesi, els mètodes de resolució multivariant, ja sigui basats en models flexibles, rígids o híbrids, s'han utilitzat àmpliament per modelitzar els processos d'interès. Els estudis d'estat estacionari han demostrat que l'estabilitat davant el pH o les variacions de temperatura de les tres diferents seqüències riques en citosina esmentades anteriorment depèn molt del nombre de parells de bases de C·C+, però també de la contribució d'altres factors, com ara la composició de la base i la longitud dels bucles. A partir d'estudis ultraràpids, el procés fotoquímic induït per la irradiació de llum UV i l'IR d'escaneig ràpid s'ha relacionat amb la formació de fotoproductes dimèrics en seqüències plegades i desplegades. L'estudi dels processos seguits mitjançant fluorescència resolta en el temps ha demostrat l’existència de més d’una espècie associada amb l’estructura i-motif en el cas de les seqüencies curtes. Finalment, l'aplicació de mètodes de resolució multivariant, basats tant en models rígids o flexibles, han permès extreure informació química valuosa dels processos evolutius de l'ADN. A més, s'ha demostrat que l'adaptació i l'aplicació del modelatge híbrid ha permet calcular les constants cinètiques i detectar transicions dependents de la temperatura.
Stumbaum, Mihaela [Verfasser]. "Development and bioanalytical application of affinity-mass spectrometry for identification and structural characterisation of protein-ligand interactions / Mihaela Stumbaum." Konstanz : Bibliothek der Universität Konstanz, 2014. http://d-nb.info/1048015424/34.
Повний текст джерелаAli, Mohsin [Verfasser]. "Bioanalytical method performance verification concept for cardiovascular research in pediatrics: From development to application in clinical trials / Mohsin Ali." Düsseldorf : Universitäts- und Landesbibliothek der Heinrich-Heine-Universität Düsseldorf, 2020. http://d-nb.info/1205544135/34.
Повний текст джерелаChan, Yannie Ka Yan. "Evaporation-induced 3-dimensional diblock copolymer micelles micropattern : applications as templated polymeric microwells for cell culture scaffold, bioanalytic arrays and micro-silver networks /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202004%20CHAN.
Повний текст джерелаIncludes bibliographical references (leaves 122-133). Also available in electronic version. Access restricted to campus users.
Zhou, Mowei. "Incorporation of Surface Induced Dissociation into a Commercial Ion Mobility - Tandem Mass Spectrometer and Application of Mass Spectrometry Methods for Structural Analysis of Non-covalent Protein Complexes." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1373977026.
Повний текст джерелаVaněčková, Tereza. "Luminiscenční nanočástice pro bioanalytické aplikace =:Luminescent nanoparticles for bioanalytical applications /." Doctoral thesis, 2019. http://www.nusl.cz/ntk/nusl-426259.
Повний текст джерелаDong, Rui. "Microfluidic devices for bioanalytical applications /." 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3250234.
Повний текст джерелаSource: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0936. Adviser: Ralph G. Nuzzo. Includes bibliographical references. Available on microfilm from Pro Quest Information and Learning.
Jian, Ting-Rui, and 簡廷叡. "Bioanalytical Applications of CuO/Cu2O/Ppy Composites." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/qzpnrq.
Повний текст джерела國立臺灣大學
化學研究所
105
Linen texture (LT) paper functionalized with CuO/Cu2O/Ppy composite has been prepared and used as an electrode for quantitation of glucose, β-galactosidase, and Escherichia coli (E. coli). The low-cost CuO/Cu2O/Ppy LT paper is prepared through a two-step process; pyrrole adsorbed on an LT paper reacts with Cu2+ in the presence of ascorbic acid and fructose to form Cu2O/Ppy that then reacts with Cu2+ in the presence of cetyltrimethylammonium bromide. The CuO/Cu2O/Ppy LT paper electrode possessing glucose oxidase-like activity catalyzes the oxidation of glucose, allowing quantitation of glucose down to 0.04 mM. Furthermore, functionalized paper size was decreased to 0.12 cm2 to fabricate the CuO/Cu2O/Ppy LT paper microelectrodes. The microelectrode enhances the mass transfer efficiency and reduces the sample volume. By taking the specificity of β-galactosidase to hydrolyze lactose to form glucose, the functionalized paper microelectrode has been employed for the quantitation of β-galactosidase, and detection of E. coli that express β-galactosidase as low as to 0.034 U mL-1 and 120 CFU mL-1, respectively. In addition, this assay is powerful for screening activators/inhibitors of β-galactosidase, and the antibiotic activities toward E. coli. Our results clearly show the merits such as low-costs, high sensitivity, specificity, and superior stability of CuO/Cu2O/Ppy LT paper (micro) electrodes for blood glucose monitor, as well as for quantitation of β-galactosidase activity and E. coli.
Koehne, Jessica E. "Applications of carbon nanotubes in bioanalytical chemistry research." Diss., 2010. http://proquest.umi.com/pqdweb?did=2065739081&sid=1&Fmt=2&clientId=48051&RQT=309&VName=PQD.
Повний текст джерелаBerggren, William Travis. "Mass spectrometry as a bioanalytical technique - applications and advancements." 2002. http://www.library.wisc.edu/databases/connect/dissertations.html.
Повний текст джерелаLewis, Erica. "Spectroscopic Studies of Cyanine Dyes and Serum Albumins for Bioanalytical Applications." 2015. http://scholarworks.gsu.edu/chemistry_theses/72.
Повний текст джерелаŠpirková, Zuzana. "Macroporous indium tin oxide as a potential platform for bioanalytical applications." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-348656.
Повний текст джерелаBird, Susan Mary. "Elemental speciation for bioanalytical applications: HPLC-ICP-MS and CE-ICP-MS." 1998. https://scholarworks.umass.edu/dissertations/AAI9909148.
Повний текст джерелаPennington, Christopher Lane. "Bioanalytical applications of electrospray ionization mass spectrometry for proteome and biomarker analysis." 2008. http://proquest.umi.com/pqdweb?did=1542114881&sid=4&Fmt=2&clientId=39334&RQT=309&VName=PQD.
Повний текст джерелаTitle from PDF title page (viewed on Nov. 26, 2008) Available through UMI ProQuest Digital Dissertations. Thesis adviser: Wood, Troy D. Includes bibliographical references.
Chen, Tse-Hsien, and 陳澤賢. "Macrocyclic Polyamine Bonded Phase and Gold Nanoparticles Self-assembled Column for Bioanalytical Applications." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/65889027822530593308.
Повний текст джерела國立臺灣大學
化學研究所
95
Two macrocyclic polyamines, [32]ane-N8 and [28]ane-N6O2 were prepared and employed as a selective modifier for the capillary electrochromatographic separation. Under the same coating process, it has been observed that [28]ane-N6O2 coated column showed higher electroosmotic flow (e.g. μeo = -9.67 × 10-4 cm2V-1s-1) than that of [32]ane-N8 (μeo = -6.19 × 10-4 cm2V-1s-1) at pH 4. It is noteworthy to mention that μeo values increase if pyridine is added into the solution of coating process. As for example, μeo increases from -1.31 × 10-4 to -4.43 × 10-4 cm2V-1s-1 at pH 6, indicating that a greater amount of the bonded ligands were found with the addition of pyridine. With [32]ane-N8 coated column, the optimum conditions for the separation of aminothiols were at -20 kV using an acetate buffer (30 mM, pH 4.0), hydrodynamic injection and detection wavelength at 214 nm. A basic mixture (pH 7.76) of sulfur-containing biomolecules was baseline separated in less than 15 min. In the similar experimental conditions, the [28]ane-N6O2 coated capillary column showed only five peaks and no characteristic splitting for reduced and oxidized form of glutathione. With pH gradient mode, the baseline separation of the acidic mixture (pH 2.73) was demonstrated using an acetate buffer (30 mM) with inlet buffer pH 4 and outlet buffer pH 3 (pH 4-3). But ethanol was needed in the mobile phase for the separation of acidic mixture (pH 1.89). A mixture of angiotensin (I、II and ST)、β-casomorphin (bovine and human)、oxytocin acetate、tocinoic acid、vasopressin and FMRF amide could be separated using phosphate buffer (pH 7, 30 mM). Column efficiency was found with the average theoretical plate numbers of 70000 m-1 and relative standard deviation (RSD) of < 1 % (n = 6) The prepared column can be used with injections more than 600 with the EOF increase only 5.91 %. [Met5]-enkephalin and [Leu5]-enkephalin could also be separated with the bonded phase in 30 mM acetate using pH gradient method (pH 4-3). The [32]ane-N8 coated column was also applied to separate of mono- and disaccharides. The reductive aminated product was carried out with p-aminobenzoic acid. A synergic effect was observed for the separation of borate complexes with the bonded phase. A complete separation of all compounds as well as the excess derivatizing agent by using borate buffer (pH 9.0) in a mode of concentration gradient (60 mM inlet side and 70 mM outlet side) could be achieved. The RSD of the migration time measured for each sample was less than 4 % in six continuous runs, suggesting that the bonded phase along with the gradient formed inside the column was quite stable. The results indicated that with the mixing modes of anion coordination, anion exchange, and shape discrimination, the interaction adequately accomplishes the separation of carbohydrates which are epimers or have different glycosidic linkage, although the electrophoretic migration is also involved in the separation mechanism. The gold nanoparticles(Au NPs) was immobilized in fused-silica capillary column wall by (3-mercaptopropyl)trimethoxysilane for the separation of sulfur-containing biomolecules. We used high concentration buffer (80 ~ 120 mM) to avoid bubble formation. Baseline separation of basic mixture was obtained at 20 kV, using an acetate buffer (80 mM, pH 4). The results suggested that the interaction between analytes and the bonded groups on the wall is ligand exchange. The Au NPs solutions were added into both 32- and 28-membered macrocyclic polyamines for realizing the status of NPs in the presence of these ligands. Spectral changes and TEM pictures demonstrate that the macrocyclic polyamines, which possess positive charges, trigger aggregation of negatively charged citrate-capped nanoparticles. Then the Au NPs self-assembled in a [32]ane-N8 coated column wall. The good separation efficiency was procured not only sulfur-containing biomolecules but also proteins include six ovalbumin peaks are observed.
Botelho, Julianne Cook. "Bioanalytical applications of mass spectrometry to identify proteins and glycans from complex biological samples." 2007. http://purl.galileo.usg.edu/uga%5Fetd/botelho%5Fjulianne%5Fm%5F200712%5Fphd.
Повний текст джерелаDirected by Ron Orlando. Includes articles submitted to Journal of proteome research and International journal of mass spectrometry. Includes bibliographical references.
Johnson, Jeremi David. "Bioanalytical applications of liquid chromatography mass spectrometry protein identification, enzyme function, carbohydrate analysis, post-translational modifications, and targeted proteomics /." 2003. http://purl.galileo.usg.edu/uga%5Fetd/johnson%5Fjeremi%5Fd%5F200308%5Fphd.
Повний текст джерелаDirected by Ron Orlando. Includes articles submitted to Journal of allergy and clinical immunology, Journal of the American Society for Mass Spectrometry, and European journal of mass spectrometry. Includes bibliographical references.
Warren, Maria Rosario Esteban. "Bioanalytical applications of mass spectrometry development and evaluation of modified probe surfaces for enhanced MALDI-TOF-MS and examination of the substrate specificity and glycosylation of A. niger pectin methylesterase by ESI-MS /." 2001. http://purl.galileo.usg.edu/uga%5Fetd/warren%5Fmaria%5Fr%5F200108%5Fphd.
Повний текст джерелаDirected by Ronald Carl Orlando. Includes articles published in Analytical chemistry, and Biochemical journal, and articles submitted to Rapid communications in mass spectrometry, and Biochemical journal. Includes bibliographical references.