Дисертації з теми "Binding component"
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Troup, Timothy J. "A component system architecture to enable user-directed component binding at run-time." Thesis, University of Glasgow, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.414045.
Повний текст джерелаWatson, Keith. "Characterisation of the dextrin 2-sulphate cell surface binding component." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265875.
Повний текст джерелаWhyte, J. "Studies on the alpha-bungarotoxin binding component in human brain." Thesis, University of Bath, 1985. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.352843.
Повний текст джерелаMarafie, Sulaiman. "TRIM7, a novel binding protein of the mTORC2 component Sin1." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/trim7-a-novel-binding-protein-of-the-mtorc2-component-sin1(c344b542-0706-4ec0-be06-ce6683cee52e).html.
Повний текст джерелаRay, Nandita. "Characterisation of an α-bungarotoxin binding component of chick optic lobe". Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/46521.
Повний текст джерелаChristner, Robert B. "Studies on the binding specifications of human serum amyloid P- component /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487847761305544.
Повний текст джерелаVaughan, P. J. "Studies on a component of the herpes simplex virus DNA polymerase." Thesis, University of Leeds, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.379648.
Повний текст джерелаRodrigues, Daniel Joseph. "Structure-function relationships in the NADP (H) binding component of proton-translocating transhydrogenase from Rhodospirillum rubrum." Thesis, Oxford Brookes University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289256.
Повний текст джерелаGrancell, Adam Scott 1969. "Biochemical characterization of CBF3, an essential DNA-binding component of the yeast kinetochore." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/49652.
Повний текст джерелаVinger, Gift. "THE STATUS OF THE PROJECTION PRINCIPLE IN GOVERNMENT-BINDING THEORY." Journal for New Generation Sciences, Vol 6, Issue 2: Central University of Technology, Free State, Bloemfontein, 2008. http://hdl.handle.net/11462/509.
Повний текст джерелаThe role of the Projection Principle within Chomsky's Government-Binding (GB) Theory is to preserve the subcategorisation properties of lexical items at all levels of syntactic representation, viz. D-structure, S-structure, and Lexical Form. Arguments have been made that the Projection Principle is a new concept that is simply an extension of theTransformational Component (XFM) and Emonds' Structure-Preserving Constraint (SPC), and that it does not deserve the high status it has been accorded in GB theory. This paper provides evidence, based on sentences involving movement operations, that the Projection Principle is innovative and that it convincingly addresses what theXFMandSPChave failed to address.
Peake, Sarah Jayne. "Structure and function of the NADP(H)-binding component (dIII) of human heart transhydrogenase." Thesis, University of Birmingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367626.
Повний текст джерелаHuxley, Lucinda. "Kinetics and specificity of nicotinamide nucleotide binding to the dIII component of transhydrogenase from Rhodospirillum Rubrum." Thesis, University of Birmingham, 2011. http://etheses.bham.ac.uk//id/eprint/1441/.
Повний текст джерелаHarris, Claire Louise. "Analysis of covalent binding reactions between human complement component C3 and other proteins of the alternative pathway." Thesis, University of Cambridge, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309802.
Повний текст джерелаMartin, Daniel Dalton. "Purification of Anthrax Toxin Protective Antigen Component and Characterization of its Binding Interaction with Bovine Kidney Cells." DigitalCommons@USU, 1986. https://digitalcommons.usu.edu/etd/4641.
Повний текст джерелаPereira, M. F. V. T., J. M. Benson, M. Williams, and H. Chikwanda. "Component and die design principles and process parameters for the metal injection moulding of a Ti alloy." Journal for New Generation Sciences, Vol 8, Issue 2: Central University of Technology, Free State, Bloemfontein, 2010. http://hdl.handle.net/11462/562.
Повний текст джерелаMetal injection moulding (MIM) offers advantages for mass production of components over conventional production methods for parts with complex shapes and large production runs. The MIM process includes mixing a fine metallic powder with a polymeric binder to produce a homogeneous feedstock. This enables the production of metallic components in a similar manner to plastic injection moulding. After undergoing a process of binder removal the components undergo a conventional sintering cycle. As significant shrinkage occurs (as much as 30%) this must be considered when designing the die cavity. This paper describes the design and manufacture of a die to produce tensile specimens. Extensive injection moulding trials to produce acceptable tensile components were undertaken. The complexities and possible implications of the design of a mould on the process are discussed. The outcomes of this research will be used by the CSIR for further development and application of the MIM technology for manufacture of high value components, such as dental implants.
Zamora, Garcia Rafael E. "Characterisation of the Doxorubicin pump of Streptomyces peucetius : the DrrA component of the DrrAB ATP-binding cassette transporter." Thesis, University of Glasgow, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.438971.
Повний текст джерелаMcHugh, Anna. "T cell responses to the E3 binding protein component of the pyruvate dehydrogenase complex in primary biliary cirrhosis." Thesis, University of Newcastle Upon Tyne, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424090.
Повний текст джерелаSmith, Adam Campbell. "Functional deficiency in the 67-kD elastin binding protein is a crucial component of the pathomechanism of Costello syndrome." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0020/MQ54081.pdf.
Повний текст джерелаHopkins, Adam P. "Molecular and biochemical characterisation of SiaP as a sialic acid binding protein component of a TRAP transporter of sialic acid." Thesis, University of York, 2010. http://etheses.whiterose.ac.uk/1030/.
Повний текст джерелаGardner, Stewart G. "Studies of PhoU in Escherichia coli: Metal Binding, Dimerization,Protein/Protein Interactions, and a Signaling Complex Model." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5685.
Повний текст джерелаMoore, Jocelyn. "Post-transcriptional control of Drosophila pole plasm component, germ cell-less." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=115700.
Повний текст джерелаJayasinghe, Wedhigangoda Arachchilage Pradeep Aruna. "Synthesis of charged receptors with a bis phenolic ether scaffold, and studies of their binding to Phosphatidylglycerol, a bacterial membrane component." Diss., Wichita State University, 2013. http://hdl.handle.net/10057/10611.
Повний текст джерелаThesis (Ph.D.)--Wichita State University, Fairmount College of Liberal Arts and Sciences, Dept. of Chemistry
Pan, Qun. "Two clusters of acidic amino acids at the NH¦2-terminus of complement component C4 Ã'-chain are important for C2 binding." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0028/MQ50359.pdf.
Повний текст джерелаGuidi, Cynthia J. "The Role of the SWI/SNF Component INI1 in Mammalian Development and Tumorigenesis: a Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/69.
Повний текст джерелаDian, Cyril. "Adaptive Responses by Transcriptional Regulators to small molecules in Prokaryotes : Structural studies of two bacterial one-component signal transduction systems DntR and HpNikR." Doctoral thesis, Stockholm : Department of Biochemistry and Biophysics, Stockholm University, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-7052.
Повний текст джерелаDayibas, Orcun. "Feature Oriented Domain Specific Language For Dependency Injection In Dynamic Software Product Lines." Master's thesis, METU, 2009. http://etd.lib.metu.edu.tr/upload/3/12611071/index.pdf.
Повний текст джерелаЗгоранець, Олег Васильович. "Робочі властивості стрижневих сумішей з комплексними зв’язувальними компонентами на основі металофосфатів". Master's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/42987.
Повний текст джерелаMaster's dissertation: 98 pp., 49 figs., 30 tables, 32 references. Objects of research: rods made of metal-phosphate mixtures of experimental composition; castings from ferrocarbon alloys (technological tests for burn and knockout). Subject of research: burns on the surface of castings; roughness of cast surfaces; beating work and hygroscopicity of rod mixtures. Purpose: to determine the set of working properties: resistance to scorching, knockout, as well as technological properties - hygroscopicity of the core mixtures, which contain binders of metal-phosphate type. Research methods: production of special model equipment; production of molds and rods, melting and pouring of metal; control of roughness of cast surfaces according to standards, definition of work of knocking out and hygroscopicity by traditional methods. The results of the research: the regularities of burn formation depending on the composition of the core mixtures and the thickness of the casting walls are established, the work of knocking out and hygroscopicity of all mixtures are determined. It is shown that rods from a number of mixtures can be used without non-stick coatings. Significance of the work: the work for the first time confirmed from a technological point of view the real possibility of using core mixtures with phosphates of silicon, aluminum, sodium, potassium, manganese for the manufacture of castings from ferrocarbon alloys. Areas of application: small casting rods (weighing up to several kg), which are hardened in a furnace or in heated equipment (150… 300 ºC, depending on the composition of the mixture), for the manufacture of castings from iron-carbon alloys. Economic efficiency - conditional economic effect UAH 44,777. Projected assumptions: gradual introduction of core mixtures, starting with shops with individual and small-scale production. Reducing the cost of casting, improving the quality of cast surfaces, sanitary and hygienic working conditions, environmental situation.
Stone, Howard C. "Isolation and characterisation of cadmium binding components of the scallop, Pecten maximus." Thesis, University of Aberdeen, 1985. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU356206.
Повний текст джерелаNordesjö, Olle. "Searching for novel protein-protein specificities using a combined approach of sequence co-evolution and local structural equilibration." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-275040.
Повний текст джерелаWhalley, Caroline. "Estimating binding strength and chemical phases of metals adsorbed to sediment components." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259990.
Повний текст джерелаCampbell, Veronica Ann. "Components of neuronal calcium channels and their interaction with GTP-binding proteins." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.363058.
Повний текст джерелаParis, Guillaume. "Effets dynamiques et conformationnels sur le rôle de transport des albumines sériques." Thesis, Besançon, 2014. http://www.theses.fr/2014BESA2024/document.
Повний текст джерелаHuman serum albumin (HSA) is a protein known for its exceptional transport properties and its high content of disulfide bridges. The study of the conformational dynamics represents a major challenge in the comprehension of its physiological functions. The aim of our work was to study the conformational dynamics and to understand the roleof disulfide bonds in the stability of the native protein structure. Our analysis is based on simulations of molecular dynamics coupled with principal component analysis. Beyond the validation of the simulation method, the results provide new insights on the main effects of the disulfide bonds reduction in serum albumins. Protein unfolding/refolding processes were detailed. A special attention is paid to the prediction of the reduced structure at the equilibrium. A detailed study of the global protein conformational dynamics as well as the two main binding sites were performed. Possible allosteric effects between these two sites were researched. The theoretical results have been discussed with the available experimental data
Whittington, Christi Leigh. "Molecular Dynamics of the RNA Binding Cavity of Influenza A Non-structural Protein 1 (NS1) RNA Binding Domain." Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4256.
Повний текст джерелаAndersson, David. "Multivariate design of molecular docking experiments : An investigation of protein-ligand interactions." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-35736.
Повний текст джерелаFajardo, Mark A. "Defining cis- and trans-acting components for Prm-1 temporal translational control during murine spermatogenesis /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/10251.
Повний текст джерелаRodríguez, Solovey Leisa Natacha. "IDENTIFICATION OF TARGETS AND AUXILIARY PROTEINS OF PYR/PYL/RCAR ABA RECEPTORS: PROTEIN PHOSPHATASES TYPE 2C (PP2Cs) AND C2-DOMAIN ABA-RELATED PROTEINS (CARs)." Doctoral thesis, Universitat Politècnica de València, 2015. http://hdl.handle.net/10251/58862.
Повний текст джерела[ES] RESUMEN La señalización por la hormona vegetal ácido abscísico (ABA) desempeña un papel crítico en la regulación del crecimiento de la raíz y en la arquitectura del sistema radical. La promoción de crecimiento de la raíz en condiciones de estrés hídrico mediada por ABA es clave para la supervivencia de las plantas bajo condiciones limitantes de agua. En este trabajo, hemos explorado el papel de los receptores PYR/PYL/RCAR (PYRABACTIN RESISTANCE1 (PYR1)/PYR1 LIKE (PYL)/ REGULATORY COMPONENTS OF ABA RECEPTORS) de Arabidopsis (Arabidopsis thaliana) en la ruta de señalización de ABA en raíz. Así, hemos descubierto que el receptor de ABA PYL8 juega un papel no redundante en la regulación de la percepción de ABA en raíz. Inesperadamente, dada la naturaleza multigénica y la redundancia funcional parcial observada en la familia PYR/PYL/RCAR, el mutante pyl8 fue el único mutante sencillo de pérdida de función de los receptores PYR/PYL/RCAR que mostraba una sensibilidad reducida a la inhibición del crecimiento mediada por ABA en raíz. Este efecto se debe a la falta de inhibición mediada por PYL8 de varias fosfatasas del grupo A tipo 2C (PP2Cs), ya que PYL8 es capaz de interactuar in vivo con al menos cinco PP2Cs, denominadas HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABAINSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 según lo han revelado la purificación por afinidad en tándem (TAP por sus siglas en inglés) y estudios proteómicos de espectrometría de masas. La transducción de la señal del ABA localizada en la membrana plasmática celular juega un papel crucial en los pasos iniciales de la señalización de la fitohormona, pero los mecanismos moleculares que unen los componentes básicos de la señalización y la membrana plasmática no están claros. Estudiando las interacciones de los receptores del ABA PYR/PYL/RCAR con la membrana plasmática hemos encontrado que éstos pueden interaccionar transitoriamente con ella de forma dependiente de calcio gracias a una familia de proteínas con dominios C2 relacionadas con la ruta de señalización de ABA (denominadas C2-domain ABA-related (CAR) proteins). Específicamente, se encontró que PYL4 interacciona de manera independiente de ABA con CAR1 tanto en la membrana plasmática como en el núcleo de las células vegetales. La proteína CAR1 pertenece a una familia multigénica constituida por 10 miembros en Arabidopsis thaliana, desde CAR1 hasta CAR10, y que solo se encuentra en plantas. Los ensayos de complementación bi-molecular de fluorescencia y de co-immunoprecipitación confirmaron la interacción en células vegetales tanto de PYL4-CAR1 como de otras parejas de PYR/PYL-CAR. La cristalización de la proteína CAR4 reveló que, además de un dominio C2 clásico de unión a lípidos dependiente de calcio, las proteínas de la familia CAR presentan un dominio específico que probablemente es responsable de la interacción con los receptores PYR/PYL/RCAR y de su posterior reclutamiento a las vesículas de fosfolípidos. Esta interacción es relevante para la función de los receptores PYR/PYL/RCAR en la señalización del ABA, ya que diferentes mutantes triples car de pérdida de función, que tienen afectados los genes CAR1, CAR4, CAR5, y CAR9, demostraron una reducción de la sensibilidad al ABA en ensayos de establecimiento de plántula y crecimiento de la raíz. En resumen, hemos identificado nueva familia de proteínas que son capaces mediar las interacciones transitorias dependientes de Ca2+ con vesículas de fosfolípidos, lo que a su vez afecta localización de PYR/PYL/RCAR y regula positivamente la señalización de ABA.
[CAT] RESUM La senyalització per l'hormona vegetal àcid abcíssic (ABA) exerceix un paper crític en la regulació del creixement de l'arrel i també en l'arquitectura del sistema radical. La promoció del creixement de l'arrel en condicions d'estrés hídric, regulada per ABA és clau per la supervivència de les plantes sota condicions limitants d'aigua. Amb aquest treball, hem investigat el paper dels receptors PYR/PYL/RCAR (PYRABACTIN RESISTANCE1 (PYR1)/PYR1 LIKE (PYL)/ REGULATORY COMPONENTS OF ABA RECEPTORS) d'Arabidopsis (Arabidopsis thaliana) en el camí de senyalització d'ABA en arrel. Així, hem descobert que el receptor d'ABA PYL8 exerceix un paper no redundant en la regulació de la percepció d'ABA en arrel. Inesperadament, donada la naturalesa multigènica i la redundància funcional parcial que s'observa en la família PYR/PYL/RCAR, el mutant pyl8 va ser l'únic mutant senzill de pèrdua de funció dels receptors PYR/PYL/RCAR que mostrava una sensibilitat reduïda a la inhibició del creixement mitjançada per l'ABA en l'arrel. Doncs aquest efecte es deu a la falta d'inhibició regulada per PYL8 de diverses fosfatases del grup A tipus 2C (PP2Cs), ja que PYL8 té la capacitat d'interactuar in vivo almenys amb cinc PP2Cs, anomenades HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABAINSENSITIVE1 (ABI1), ABI2, and PP2CA/ABAHYPERSENSITIVE GERMINATION3 segons ho han revelat per una banda la purificació per afinitat en tàndem (TAP són les seues sigles en anglés) i per altra banda, estudis proteòmics d'espectrometria de masses. Pel que fa a la transducció del senyal del l'ABA, la qual es localitza en la membrana plasmàtica cel¿lular, juga un paper molt important en els primers instants de la senyalització de la fitohormona, no obstant això els mecanismes moleculars que uneixen els components bàsics d'aquesta senyalització amb la membrana plasmàtica, no es troben del tot clars. Per tant, s'han estudiat les interaccions que tenen els receptors del ABA PYR/PYL/RCAR amb la membrana plasmàtica, i hem trobat que aquests tenen la capacitat d'interaccionar transitòriament amb la membrana de forma dependent al calci, gràcies a una família de proteïnes amb domini C2, les quals es troben relacionades amb la ruta de senyalització d'ABA(anomenades C2domain ABArelated (CAR) proteins).Específicament, es va trobar que PYL4 interacciona d'una manera independent al ABA amb CAR1, tant en la membrana plasmàtica, com en el nucli de les cèl¿lules vegetals. La proteïna CAR1 pertany a la família multigènica constituïda per 10 components en Arabidopsis thaliana, des de CAR1 fins CAR10, que tan sols es troba en plantes. Els assajos de complementació bimolecular de fluorescència i de co-immunoprecipitació, van confirmar la interacció en cèl¿lules vegetals, tant de PYL4CAR1 com d'altres parelles de PYR/PYL-CAR. La cristal¿lització de la proteïna CAR4 va revelar que, a més d'un domini C2 clàssic de unió a lípids dependent del calci, les proteïnes de la família CAR presenten un domini PYR/PYL/RCAR, i del seu posterior reclutament a les vesícules fosfolipídiques. Doncs, aquesta interacció és rellevant en la funció dels receptors PYR/PYL/RCAR, ja que participa en la senyalització del l'ABA. Aquesta interacció es clau per a la funció dels receptors, ja que diferents mutants triples car de pèrdua de funció, els quals posseïxen afectats els gens CAR1, CAR4, CAR5 i CAR9, van mostrar una reducció de la sensibilitat a l'ABA en assajos d'establiment de plàntula i creixement de l'arrel. En conclusió, hem identificat una nova família de proteïnes amb la capacitat d'organitzar les interaccions transitòries dependents del calci amb vesícules de fosfolípids, fet que al seu torn afecta la localització de PYR/PYL/RCAR i regula positivament la senyalització d'ABA.
Rodríguez Solovey, LN. (2015). IDENTIFICATION OF TARGETS AND AUXILIARY PROTEINS OF PYR/PYL/RCAR ABA RECEPTORS: PROTEIN PHOSPHATASES TYPE 2C (PP2Cs) AND C2-DOMAIN ABA-RELATED PROTEINS (CARs) [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/58862
TESIS
Flory, Mark Randall. "Isolation and characterization of calmodulin-binding centrosome components related to Saccharomyces cerevisiae Spc110p from the fission yeast Schizosaccharomyces pombe and humans /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/5061.
Повний текст джерелаDunaiski, Vera. "Effects of IGF-1 or LR3IGF-1 infusion on components of the GH/IGF-1 axis in pigs /." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phd897.pdf.
Повний текст джерелаKaczmarczyk, Aneta. "Proteins of the Inter-α-inhibitor Family : Biosynthesis, Plasma Clearance and Interaction with Extracellular Matrix Components". Doctoral thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3495.
Повний текст джерелаBikunin, a chondroitin sulfate-containing protein of 25 kDa, has protease inhibitory activity and occurs in the plasma in free and complexed form. In inter-α-inhibitor (IαI) and pre-a-inhibitor (PαI) it is covalently linked through its chondroitin sulfate (CS) chain to two or one other polypeptide of about 80 kDa – heavy chains 1 and 2 (H1, H2) and heavy chain 3 (H3) – respectively. Bikunin and the heavy chains are synthesized as precursors, which are proteolytically cleaved and assembled into IαI and PαI in the secretory pathway. The C-terminal extension (CTX) of the heavy chains seems to mediate its own cleavage and theassembly of the complexes. The heavy chains of the IαI family become transferred to hyaluronan during ovulation and inflammation.
In this thesis, the biosynthesis of PαI, the plasma clearance of bikunin and the binding of IαI to collagen were studied. We found that in H3, a short segment on the N-terminal side of the CTX cleavage site is required for cleavage. Furthermore, the H3 could become linked to free CS chains primed by a xyloside, showing that the bikunin protein core is not needed for coupling. We also identified His649 as a residue essential for coupling, but not for cleavage.
Bikunin labelled with a residualizing agent, 125I-tyramine cellobiose, was injected into mice to identify tissues involved in its uptake. Half of the radioactivity was recovered in the kidneys, 10% in the liver, and the rest distributed in other tissues. We determined the half-life of bikunin in rat plasma using two independent methods: injection of 125I-bikunin, or hepatectomy followed by assessing the rate of disappearance of endogenous bikunin. Both methods yielded half-time values of 5-7 minutes. Removal of the CS chain did not affect the clearance rate of bikunin.
IαI and its heavy chains were found to bind to collagen with dissociation constants greater than 2 μM and 0.4-0.6 μM, respectively and this binding was independent of divalent metal ions. We suggest that the interaction of IαI with collagen may play a modulatory role in cell migration or in remodelling of the extracellular matrix.
Molento, Carla Forte Maiolino. "Effects of insulin and the interaction between insulin and recombinant bovine somatotropin on the production of milk and its components and on IGF-I plasma levels." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=38085.
Повний текст джерелаTejedor, Vaquero Juan Ramón 1984. "Systematic functional analyses of spliceosomal components reveal novel mechanisme of alternative splicing regulation." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/385718.
Повний текст джерелаEl procesamiento alternativo del pre-ARNm constituye uno de los pilares esenciales en la regulación de la expresión génica y expande la capacidad codificadora del genoma en organismos multicelulares. El Espliceosoma – la maquinaria encargada de la eliminación alternativa de los intrones- permite una regulación multifacética de los programas genéticos en el interior de la célula. El proceso de corte y empalme se sustenta en la interacción dinámica de cientos de componentes del Espliceosoma, y los distintos niveles de regulación de la compleja reacción de splicing permanecen aun sin descubrir. El objetivo principal de esta tesis se ha centrado en el desarrollo de tecnologías sistematicas de alto cribado para identificar reguladores potenciales del procesamiento alternativo del pre- ARNm, así como los mecanismos implicados en su regulación. Hemos identificado una gran variedad de reguladores del procesamiento alternativo de Fas/CD95, tanto componentes esenciales del espliceosoma como factores implicados en otros procesos biologicos, y hemos observado una conexión inédita entre la regulación del splicing alternativo y el proceso de homeostasis modulado por hierro. Mediante el uso de redes computacionales, hemos llevado a cabo un análisis sistemático y funcional de los componentes del Espliceosoma y hemos identificado el potencial regulador de los mismos en la reacción de corte y empalme. Nuestros resultados reflejan una inmensa plasticidad de los factores esenciales del Espliceosoma a lo largo de toda a reacción de ensamblaje. Además, hemos conseguido identificar el mecanismo potencial por el cual la homeostasis del hierro ejerce su función en splicing alternativo a través de la modulación de la actividad de unión a RNA -mediada por un dominio de unión a cinc- en la proteína reguladora de splicing SRSF7. Los resultados de esta tesis enfatizan la relevancia de las tecnologías emergentes de alto cribado y el análisis de redes computacionales en el estudio de complejos mecanismos moleculares, y desvelan nuevas conexiones funcionales entre la maquinaria de splicing y otros procesos celulares.
Bertacchini, Eleonora. "Molecular study of stress system in the European sea bass (Dicentrarchus labrax): cloning of different components and effects of essential oil of Lippia alba during stress situation." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
Знайти повний текст джерелаLabusch, Corinna [Verfasser]. "Patatin-related phospholipases (pPLA) and other cytosolic components in auxin binding protein1 (ABP1)-mediated auxin signaling are revealed by using early auxin-induced gene expression as a biotest in Arabidopsis thaliana / Corinna Labusch." Hannover : Technische Informationsbibliothek und Universitätsbibliothek Hannover (TIB), 2013. http://d-nb.info/1041238843/34.
Повний текст джерелаLindström, Anton. "A multivariate approach to characterization of drug-like molecules, proteins and the interactions between them." Doctoral thesis, Umeå universitet, Kemi, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1924.
Повний текст джерелаA disease is often associated with a cascade reaction pathway involving proteins, co-factors and substrates. Hence to treat the disease, elements of this pathway are often targeted using a therapeutic agent, a drug. Designing new drug molecules for use as therapeutic agents involves the application of methods collectively known as computer-aided molecular design, CAMD. When the three dimensional (3D) geometry of a macromolecular target (usually a protein) is known, structure-based CAMD is undertaken and structural information of the target guides the design of new molecules and their interactions with the binding sites in targeted proteins. Many factors influence the interactions between the designed molecules and the binding sites of the target proteins, such as the physico-chemical properties of the molecule and the binding site, the flexibility of the protein and the ligand, and the surrounding solvent. In order for structure-based CAMD to be successful, two important aspects must be considered that take the abovementioned factors into account. These are; i) 3D fitting of molecules to the binding site of the target protein (like fitting pieces of a jigsaw puzzle), and ii) predicting the affinity of molecules to the protein binding site. The main objectives of the work underlying this thesis were: to create models for predicting the affinity between a molecule and a protein binding site; to refine the geometry of the molecule-protein complex derived by or in 3D fitting (also known as docking); to characterize the proteins and their secondary structure; and to evaluate the effects of different generalized-Born (GB) and Poisson-Boltzmann (PB) implicit solvent models on the refinement of the molecule-protein complex geometry created in the docking and the prediction of the molecule-to-protein binding site affinity. A further objective was to apply chemometric methodologies for modeling and data analysis to all of the above. To summarize, this thesis presents methodologies and results applicable to structure-based CAMD. Results show that predictive chemometric models for molecule-to-protein binding site affinity could be created that yield comparable results to similar, commonly used methods. In addition, chemometric models could be created to model the effects of software settings on the molecule-protein complex geometry using software for molecule-to-binding site docking. Furthermore, the use of chemometric models provided a more profound understanding of protein secondary structure descriptors. Refining the geometry of molecule-protein complexes created through molecule-to-binding site docking gave similar results for all investigated implicit solvent models, but the geometry was significantly improved in only a few examined cases (six of 30). However, using the geometry-refined molecule-protein complexes was highly valuable for the prediction of molecule-to-binding site affinity. Indeed, using the PB solvent model it yielded improvements of 0.7 in correlation coefficients (R2) for binding affinity parameters of a set of Factor Xa protein drug molecules, relative to those obtained using the fitting software.
López, Muñoz Laura. "Homology modeling and structural analysis of the antipsychotic drugs receptorome." Doctoral thesis, Universitat Pompeu Fabra, 2010. http://hdl.handle.net/10803/7228.
Повний текст джерелаThe study started with obtaining homology models for all the receptors putatively involved in the antipsychotic drugs receptorome, suitable for building consistent drug-receptor complexes. These complexes were structurally analyzed and compared using multivariate statistical methods, which in turn allowed the identification of the relationship between the pharmacological properties of the antipsychotic drugs and the structural differences in the receptor targets. The results can be exploited for the design of safer and more effective antipsychotic drugs with an optimum binding profile.
Tradicionalmente se asumía que los fármacos terapéuticamente efectivos actuaban interaccionando con un único receptor. Actualmente está ampliamente reconocido que el efecto farmacológico de la mayoría de los fármacos es más complejo y abarca a un conjunto de receptores, algunos asociados a los efectos terapéuticos y otros a los secundarios y toxicidad. Los fármacos antipsicóticos son un ejemplo de compuestos eficaces que se caracterizan por unirse a varios receptores simultáneamente (principalmente a receptores unidos a proteína G, GPCR). El trabajo de la presente tesis se ha centrado en el estudio de los mecanismos moleculares que determinan el perfil de afinidad de unión por múltiples receptores de los fármacos antipsicóticos.
En primer lugar se construyeron modelos de homología para todos los receptores potencialmente implicados en la actividad farmacológica de dichos fármacos, usando una metodología adecuada para construir complejos fármaco-receptor consistentes. La estructura de estos complejos fue analizada y se llevó a cabo una comparación mediante métodos estadísticos multivariantes, que permitió la identificación de asociaciones entre la actividad farmacológica de los fármacos antipsicóticos y diferencias estructurales de los receptores diana. Los resultados obtenidos tienen interés para ser explotados en el diseño de fármacos antipsicóticos con un perfil farmacológico óptimo, más seguros y eficaces.
Marcon, Edyta. "RNA binding protein, MIWI, a component of the synaptonemal complex /." 2004. http://wwwlib.umi.com/cr/yorku/fullcit?pMQ99355.
Повний текст джерелаTypescript. Includes bibliographical references (leaves 48-64). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pMQ99355
Chen, Yen-Chi, and 陳彥錡. "Biochemical Binding Characterization of Human Progesterone Receptor Membrane Component 1." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/46377342883670419342.
Повний текст джерела國立屏東教育大學
化學生物系碩士班
101
Human progesterone receptor membrane protein (Progesterone Receptor Membrane Component 1 , PGRCM 1) the study was to investigate the protein; Recent studies have pointed out, PGRMC1 Council and heme binding, but its specific physiological role has not yet been identified in the organism. In addition, although the name PGRCM 1 literature that progesterone receptor membrane protein for the human, but in fact do not directly bind progesterone, but with an unknown protein steroid / drug binding protein (S/D-BP) for Progesterone binding message passing and metabolism; expressed concern for the results of this literature. Therefore, in order to further understand the PGRCM 1 and progesterone combined with the actual situation, this study lab colleagues previously applied transgenic technology in Escherichia coli (E.coli) in a large number of performance PGRMC1; and were added to the culture of Heme precursor ALA (5-Aminolevul Acid Hydrochloride) in the culture medium; and in developing purification after adding hemin (Hemin Chloride) reconstruction, obtained by these two methods PGRMC1 iron containing metal center and comparison. We further use of UV - visible spectroscopy (UV-Vis) confirm PGRMC1 with metal centers of iron oxidation state (Fe3 +), and the use of quartz crystal microbalance QCM (Quartz Crystal Microbala) analysis of the binding constant between progesterone; also Discussion between PGRMC1 and binding of Heme. The results of this thesis show, PGRMC1 does not directly bind with Progesterone, but PGRMC1 active center Heme reconstruction may impact combined with Progesterone situation; while PGRMC1 and Heme Kd value can be achieved between 10-8.
Wang, Li Jie, and 王立傑. "Interactome-wide Analysis Identifies End-Binding Protein 1 as a Crucial Component For The Speck-like Particle Formation of Activated Inflammasomes." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/37074499986072960485.
Повний текст джерела長庚大學
生物醫學研究所
100
Inflammasomes are cytoplasmic receptors that can recognize intracellular pathogens or danger signals and are critical for IL-1β production. Although several key components of inflammasome activation have been identified, there has not been a systematic analysis of the protein components found in the stimulated complex. In this study, we used the isobaric tags for relative and absolute quantification (iTRAQ) approach to systemically analyze the interactomes of the NLRP3, AIM2 and RIG-I inflammasomes in NPC cells treated with specific stimuli of these interactomes (H2O2, poly (dA:dT) and EBER, respectively). We identified a number of proteins that appeared to be involved in the interactomes and also could be precipitated with anti-ASC antibodies after stimulation. Among them, EB1 was an interacting component in all three interactomes. Silencing of EB1 expression by small interfering RNA inhibited the activation of the three inflammasomes, as indicated by reduced levels of IL-1β secretion. We confirmed that EB1 directly interacted with AIM2 and ASC in vitro and in vivo. Most importantly, fluorescence confocal microscopy showed that EB1 was required for formation of the speck-like particles that represent activation of the AIM2 inflammasome. In NPC tissues, IHC staining showed that EB1 expression was elevated and significantly correlated with AIM2 and ASC expression in NPC tumor cells. In sum, we profiled the interactome components of three inflammasomes and show for the first time that EB1 is crucial for the speck-like particle formation that represents activated inflammasomes.
Li, Pai-Chi, and 李珮琦. "Theoretical Study of The “Charge Center-Linker-Hydrogen Binding Site” Three-Component Signal Transducers: The Effect of Charge-Bearing Substituents and Counter Ions." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/11521827295153390809.
Повний текст джерела國立臺灣大學
化學研究所
89
We consider a charge-bearing molecule as a three-component system consisting of a charge center, a linker, and a hydrogen bond binding site. In the three-component system, the binding center is controlled by a reaction center (charge center). Basically, we want to maximize the long-range through-bond effect and turn the three-component system into an effective signal transducer. In real experiment, one can change the formal charge of the reaction center through protonation, oxidation, reduction, or chemical transformation of a functional group. In our theoretical study, protonation was calculated for the reaction site. For the linker part, fully conjugated alkenes, alkynes and -N=N- are good candidates for maximizing through-bond effects. For a binding site, pyrrole was chosen for its ability to donate electrons through conjugation with the linker. Our aim is to investigate how different reaction centers and counter ions affect the hydrogen bonding sites, so as to provide guidelines for the design of effective signal transducers. Calculation results at the HF/6-31+G* level showed that 1) for neutral species, the longer the linker unit, the larger its binding energy (5~6 kcal/mol); for charged species, the binding energy of the protonated cationic system was roughly double that of the neutral system (9~14 kcal/mol). 2) If the linker is -(CH=CH)n-, the longer the linker unit is, the less binding energy it has. With the azo linkers, we have demonstrated that it is even possible to amplify the signals when the charge center is an iminium group (-CH=NH2+). 3) Counter ions could decrease the binding energy of the binding site because of long-range electrostatic interactions and because some electron is transferred from the counter ion to the reaction center. The transducers containing iminium are greatly influenced by the counter ions, while the charge delocalized guanidinium group (-NHC(NH2)2+), whose electrons can not conjugate with the linker, seem to have better ability to preserve signal transduction in the presence of a counter ion (40~50%). The calculation results showed that the efficiency of changing counter ions to improve signal transduction is quite small. Experimentally, one could consider reducing the effect of counter ions by using anion-binding hosts.
Wani, Saima Masood. "Elucidation of the Role of Nse1, a RING Domain Containing Component of Smc5/6 complex, in Maintenance of Chromosome Stability in Saccharomyces cerevisiae." Thesis, 2017. http://etd.iisc.ernet.in/2005/3570.
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