Готові списки джерел за темами / Bead-based bioassay

Добірка наукової літератури з теми "Bead-based bioassay"

Автор: Grafiati
Опубліковано: 14 березня 2023

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Зміст

Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "Bead-based bioassay".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Статті в журналах з теми "Bead-based bioassay"

1

Tripodi, Lisa, Karen Ven, Dries Kil, Iene Rutten, Robert Puers, and Jeroen Lammertyn. "Teflon-on-Glass Molding Enables High-Throughput Fabrication of Hydrophilic-in-Hydrophobic Microwells for Bead-Based Digital Bioassays." Materials 11, no. 11 (November 1, 2018): 2154. http://dx.doi.org/10.3390/ma11112154.

Повний текст джерела
Анотація:
In recent years, Teflon-on-glass microwells have been successfully implemented in bead-based digital bioassays for the sensitive detection of single target molecules. Their hydrophilic-in-hydrophobic (HIH) nature enables the isolation and analysis of individual beads, carrying the target molecules, which can be further manipulated accurately through optical tweezer (OT) setups. However, these Teflon HIH-microwell platforms are conventionally fabricated through a complex, time-consuming and labor-intensive dry lift-off procedure which involves a series of major steps, limiting the up-scaling potential of these platforms. Alternative Teflon-based microwell fabrication methods have been extensively explored in literature but they preclude the generation of hydrophobic wells with hydrophilic bottom, thereby hampering the bioassay performance. Here, we present a new Teflon-on-glass molding method for the high throughput fabrication of hydrophilic-in-hydrophobic (HIH) microwell arrays, able to empower bead-based digital bioassays. Microwells 2.95 μm in depth and 3.86 μm in diameter were obtained to host individual beads. In these microwell arrays, sealing of reagents was demonstrated with an efficiency of 100% and seeding of superparamagnetic beads was achieved with an efficiency of 99.6%. The proposed method requires half as many steps when compared to the traditional dry lift-off process, is freely scalable and has the potential to be implemented in different bead-based bioassay applications.
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Bettazzi, Francesca, Ezat Hamid-Asl, Carla Lucia Esposito, Cristina Quintavalle, Nello Formisano, Serena Laschi, Silvia Catuogno, et al. "Electrochemical detection of miRNA-222 by use of a magnetic bead-based bioassay." Analytical and Bioanalytical Chemistry 405, no. 2-3 (October 26, 2012): 1025–34. http://dx.doi.org/10.1007/s00216-012-6476-7.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Aytur, Turgut, Jonathan Foley, Mekhail Anwar, Bernhard Boser, Eva Harris, and P. Robert Beatty. "A novel magnetic bead bioassay platform using a microchip-based sensor for infectious disease diagnosis." Journal of Immunological Methods 314, no. 1-2 (July 2006): 21–29. http://dx.doi.org/10.1016/j.jim.2006.05.006.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Azhar, Umar, Qazi Ahmed, Saira Ishaq, Zeyad T. Alwahabi, and Sheng Dai. "Exploring Sensitive Label-Free Multiplex Analysis with Raman-Coded Microbeads and SERS-Coded Reporters." Biosensors 12, no. 2 (February 16, 2022): 121. http://dx.doi.org/10.3390/bios12020121.

Повний текст джерела
Анотація:
Suspension microsphere immunoassays are rapidly gaining attention in multiplex bioassays. Accurate detection of multiple analytes from a single measurement is critical in modern bioanalysis, which always requires complex encoding systems. In this study, a novel bioassay with Raman-coded antibody supports (polymer microbeads with different Raman signatures) and surface-enhanced Raman scattering (SERS)-coded nanotags (organic thiols on a gold nanoparticle surface with different SERS signatures) was developed as a model fluorescent, label-free, bead-based multiplex immunoassay system. The developed homogeneous immunoassays included two surface-functionalized monodisperse Raman-coded microbeads of polystyrene and poly(4-tert-butylstyrene) as the immune solid supports, and two epitope modified nanotags (self-assembled 4-mercaptobenzoic acid or 3-mercaptopropionic acid on gold nanoparticles) as the SERS-coded reporters. Such multiplex Raman/SERS-based microsphere immunoassays could selectively identify specific paratope–epitope interactions from one mixture sample solution under a single laser illumination, and thus hold great promise in future suspension multiplex analysis for diverse biomedical applications.
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Heade, Joanne, Fiona McCartney, Miguel Chenlo, Olga Moreno Marro, Maja Severic, Robert Kent, Sinead B. Bleiel, Clara V. Alvarez, Brendan T. Griffin, and David J. Brayden. "Synthesis and In Vivo Evaluation of Insulin-Loaded Whey Beads as an Oral Peptide Delivery System." Pharmaceutics 13, no. 5 (May 4, 2021): 656. http://dx.doi.org/10.3390/pharmaceutics13050656.

Повний текст джерела
Анотація:
For many diabetics, daily, lifelong insulin injections are required to effectively manage blood glucose levels and the complications associated with the disease. This can be a burden and reduces patient quality of life. Our goal was to develop a more convenient oral delivery system that may be suitable for insulin and other peptides. Insulin was entrapped in 1.5-mm beads made from denatured whey protein isolate (dWPI) using gelation. Beads were then air-dried with fumed silica, Aerosil®. The encapsulation efficiency was ~61% and the insulin loading was ~25 µg/mg. Dissolution in simulated gastric-, and simulated intestinal fluids (SGF, SIF) showed that ~50% of the insulin was released from beads in SGF, followed by an additional ~10% release in SIF. The omission of Aerosil® allowed greater insulin release, suggesting that it formed a barrier on the bead surface. Circular dichroism analysis of bead-released insulin revealed an unaltered secondary structure, and insulin bioactivity was retained in HepG2 cells transfected to assess activation of the endogenous insulin receptors. Insulin-entrapped beads were found to provide partial protection against pancreatin for at least 60 min. A prototype bead construct was then synthesised using an encapsulator system and tested in vivo using a rat intestinal instillation bioassay. It was found that 50 IU/kg of entrapped insulin reduced plasma glucose levels by 55% in 60 min, similar to that induced by subcutaneously (s.c.)-administered insulin (1 IU/kg). The instilled insulin-entrapped beads produced a relative bioavailability of 2.2%. In conclusion, when optimised, dWPI-based beads may have potential as an oral peptide delivery system.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Johdi, Nor Adzimah, Hanif Zulkhairi Mohd Said, Mohd Razif Mohd Idris, Nor Azimah Ismail, Wan Fariza Wan Jamaludin, and S. Fadila Abd Wahid. "Cytokine Profiling as A Potential Biomarker in Diffuse Large B-Cell Lymphoma." Journal of Biochemistry, Microbiology and Biotechnology 8, no. 2 (December 31, 2020): 1–6. http://dx.doi.org/10.54987/jobimb.v8i2.534.

Повний текст джерела
Анотація:
Cytokines are small proteins that mediate and regulate immunity. They are involved in the pathogenesis of many diseases including cancers. The concentration of these proteins in biological fluids (serum or plasma) and tissues in diseases may suggest pathway activation that leads to inflammatory response or disease progression. Therefore, these cytokines may be useful as a tool for screening, diagnosis classification between stages of disease or surveillance for therapy. Enzyme-linked immunosorbent assays (ELISA) and bioassay have been used as a gold standard in cytokine level measurements in clinical practice. However, these methods allow only single cytokine detection at a time and ineffective for screening purposes. Hence, the innovation of multiplexing technology allows measurement of many of these soluble proteins simultaneously, thus allowing rapid, cost-effective and better efficiency by using a minute amount of sample. In this study, we explored the profiles of key inflammatory cytokines from the serum derived from diffuse large b-cell lymphoma (DLBCL, n =11) and healthy volunteers (N, n =11) using multiplexed bead-based immunoassays. We aimed to evaluate if the levels of these cytokines are significantly different in these two groups and explore the possible application of the cytokine as biomarkers in early-stage screening and/or surveillance. Our results show a significantly high level of IL-17A, IL-10 and IL-6 in DLBCL-derived serum compared to n-derived serum. These preliminary results were obtained from a small sample size and could be further validated with a larger sample size cohort to produce a panel of biomarkers for DLBCL. Our findings might be useful in developing a disease-specific panel for biomarker screening assay. This could be used for early diagnosis and/or treatment surveillance.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Linz, Thomas H., W. Hampton Henley, and J. Michael Ramsey. "Photobleaching kinetics-based bead encoding for multiplexed bioassays." Lab on a Chip 17, no. 6 (2017): 1076–82. http://dx.doi.org/10.1039/c6lc01415a.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Glynne-Jones, P., R. J. Boltryk, M. Hill, F. Zhang, L. Dong, J. S. Wilkinson, T. Brown, T. Melvin, and N. R. Harris. "Multi-modal particle manipulator to enhance bead-based bioassays." Physics Procedia 3, no. 1 (January 2010): 269–75. http://dx.doi.org/10.1016/j.phpro.2010.01.036.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Glynne-Jones, P., R. J. Boltryk, M. Hill, F. Zhang, L. Dong, J. S. Wilkinson, T. Brown, T. Melvin, and N. R. Harris. "Multi-modal particle manipulator to enhance bead-based bioassays." Ultrasonics 50, no. 2 (February 2010): 235–39. http://dx.doi.org/10.1016/j.ultras.2009.09.025.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Wang, Kuan-Chih, Aloke Kumar, Stuart J. Williams, Nicolas G. Green, Kyung Chun Kim, and Han-Sheng Chuang. "An optoelectrokinetic technique for programmable particle manipulation and bead-based biosignal enhancement." Lab Chip 14, no. 20 (2014): 3958–67. http://dx.doi.org/10.1039/c4lc00661e.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
Більше джерел

Дисертації з теми "Bead-based bioassay"

1

CALMO, ROBERTA. "Development of Monolithic Glass Suspended Microchannel Resonators designed for bead-based bioassays." Doctoral thesis, Politecnico di Torino, 2019. http://hdl.handle.net/11583/2742528.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Частини книг з теми "Bead-based bioassay"

1

Taber, Douglass F. "Developments in Flow Chemistry." In Organic Synthesis. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190646165.003.0017.

Повний текст джерела
Анотація:
Klavs S. Jensen of MIT showed (Angew. Chem. Int. Ed. 2014, 53, 470) that “batch” kinetics could be developed in flow by online IR analysis and continuous control. Professor Jensen also demonstrated (Org. Process Res. Dev. 2014, 18, 402) the contin­uous flow production of an active pharmaceutical product, the direct renin inhibitor aliskiren, over two steps and two crystallizations, starting from two advanced interme­diates. Michael Werner and Rainer E. Martin of Hoffmann-La Roche AG Basel com­bined (Angew. Chem. Int. Ed. 2014, 53, 1704) flow synthesis with a flow-based bioassay to develop structure–activity relationships for a series of β-secretase inhibitors. Carlos Mateos of Lilly S. A. and C. Oliver Kappe of the University of Graz used (J. Org. Chem. 2014, 79, 223) flow photolysis to promote the bromination of 1 to 2. Alessandro Palmieri of the University of Camerino and Stefano Protti of the University of Pavia added (Adv. Synth. Catal. 2014, 356, 753) the aldehyde 3 to the acceptor 4 to give, after in-flow reduction, the lactone 5. Peter H. Seeberger of the Max Planck Institute Mühlenberg showed (Org. Lett. 2014, 16, 1794) that the tum­bling action of flow photolysis made the production of 7 by the unlinking of 6 from the polymer bead particularly efficient. Enzymes can also be used under flow conditions. Jörg Pietruszka of the Heinrich-Heine-Universität Düsseldorf employed (Adv. Synth. Catal. 2014, 356, 1007) com­mercial laccase to prepare 10 by coupling 8 with 9. Gas–liquid mixing under flow conditions can also be effective. Núria López of ICIQ Catalonia and Javier Pérez-Ramírez of ETH Zurich developed (Chem. Eur J. 2014, 20, 5926) conditions for the selective hydrogenation of an alkyne 11 to the cis alkene 12. Jun-ichi Yoshida of Kyoto University trapped (Chem. Eur J. 2014, 20, 7931) the inter­mediate organolithium from 13 with CO₂ to give a carboxylate that was carried on to the purifiable O-Su ester 14, ready for further coupling. Timothy F. Jamison, also of MIT, prepared (Angew. Chem. Int. Ed. 2014, 53, 3353) the amino phenol 17 by add­ing the chloromagnesium amide from 16 to the intermediate benzyne from 15, then oxidizing the product with air.
Стилі APA, Harvard, Vancouver, ISO та ін.

Тези доповідей конференцій з теми "Bead-based bioassay"

1

Grumann, Markus, Patric Schippers, Michael Dobmeier, Stefan Ha¨berle, Andreas Geipel, Thilo Brenner, Roland Zengerle, Jens Ducre´e, Claus Kuhn, and Michael Fritsche. "Formation of Hexagonal Monolayers by Flow of Bead Suspensions Into Flat Microchambers." In ASME 2003 International Mechanical Engineering Congress and Exposition. ASMEDC, 2003. http://dx.doi.org/10.1115/imece2003-41427.

Повний текст джерела
Анотація:
To realize a highly parallel optical detection in bead-based bioanalytical assays, we investigate the hydrodynamic aggregation of bead suspensions in a hexagonally periodical monolayer by a pressure-driven flow through a microfluidic structure. This device consists of one inlet channel connected to a shallow chamber with a depth that only slightly exceeds the diameter of the beads. To enforce the aggregation of the beads, the flow leaves the chamber via outlet channels possessing a depth smaller than a bead diameter. This way the outlets act as barriers to the beads and force them to accumulate in the chamber. Benchmarking different chamber and outlet designs we found an optimum filing behavior for a rhombus-like aggregation chamber connected to a single outlet channel at the same width as the chamber. Here, the aperture angle of 60° fosters hexagonal aggregation patterns which leads to the highest packaging density. Reproducible filling ratios of more than 94% have been achieved. The rhombus-like chamber also shows the shows the smallest increase of the hydrodynamic resistance during filling and the best rinsing behavior which allows to minimize the volume of washing detergents used for a bioassay. Zones of accumulated beads redistribute the hydrodynamic flow through the device during the filling process. CFD-simulations, embedded in an iterative master-routine, are carried out to describe the complete process of filling and to assist the process of design optimization.
Стилі APA, Harvard, Vancouver, ISO та ін.
Також вас можуть зацікавити розширені добірки на тему "Bead-based bioassay" для конкретних типів джерел:
Статті в журналах
Ми пропонуємо знижки на всі преміум-плани для авторів, чиї праці увійшли до тематичних добірок літератури. Зв'яжіться з нами, щоб отримати унікальний промокод!

До бібліографії