Дисертації з теми "Bacterial genetic transformation"
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Cook, Marisa Anne. "Replicons derived from endogenously isolated plasmids used to classify plasmids occurring in marine sediment bacteria." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/25736.
Повний текст джерелаHazen, Tracy Heather. "Genetic elements and molecular mechanisms driving the evolution of the pathogenic marine bacterium Vibrio parahaemolyticus." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/29611.
Повний текст джерелаCommittee Chair: Patricia Sobecky; Committee Member: Eric Stabb; Committee Member: Jim Spain; Committee Member: Roger Wartell; Committee Member: Thomas DiChristina. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Fullner, Karla Jean. "The pilus assembly and T-DNA transfer machinery of Agrobacterium tumefaciens /." Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/11497.
Повний текст джерелаCaro, Quintero Alejandro. "The role of horizontal gene transfer in bacterial evolution." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/48979.
Повний текст джерелаAntonova, Elena S. "The regulatory network controlling natural competence for DNA uptake in Vibrio cholerae." Diss., Georgia Institute of Technology, 2013. http://hdl.handle.net/1853/47626.
Повний текст джерелаParsons, Stephen H. "Comparing orchid transformation using agrobacterium tumefaciens and particle bombardment." Virtual Press, 1995. http://liblink.bsu.edu/uhtbin/catkey/941350.
Повний текст джерелаDepartment of Biology
Hutchinson, Chad M. "Agrobacterium tumefaciens mediated transformation of orchid tissue with the sense and antisense coat protein genes from the odontoglossum ringspot virus." Virtual Press, 1992. http://liblink.bsu.edu/uhtbin/catkey/834608.
Повний текст джерелаDepartment of Biology
Jani, Mehul. "Genomic Island Discovery through Enrichment of Statistical Modeling with Biological Information." Thesis, University of North Texas, 2018. https://digital.library.unt.edu/ark:/67531/metadc1248417/.
Повний текст джерелаSaavedra, De Bast Manuel. "Systèmes Ta de la famille ccd, de simples gènes égoïstes?" Doctoral thesis, Universite Libre de Bruxelles, 2009. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210045.
Повний текст джерелаEntre-temps, la compréhension de l’évolution des génomes bactériens a connu des avancées significatives. L’impressionnante capacité d’adaptation des bactéries est aujourd’hui majoritairement attribuée au transfert horizontal de gènes (THG) provoqué par les éléments génétiques mobiles (phages, plasmides, transposons…). Dans le débat du rôle des systèmes TA chromosomiques, très peu d’attention a été accordée aux relations phylogénétiques et interactions entre systèmes plasmidiques et chromosomiques co-existant au sein d’un même hôte ainsi qu’à l’impact du THG sur leur évolution. Notre travail de thèse vise à mieux comprendre la biologie des systèmes TA en tenant compte de ces paramètres. Nous nous sommes intéressés à des systèmes homologues au système plasmidique ccdF. Nous avons étudié expérimentalement les 4 systèmes ccd (ccd1, ccd2, ccd3 et ccd4) qui co-habitent au sein du chromosome d’Erwinia chrysanthemi 3937 (une bactérie phytopathogène), leurs interactions intragénomiques et les interactions de ces systèmes avec le système plasmidique ccdF. Ce cadre expérimental a mené à la construction du modèle d’anti-addiction. Ce modèle propose que certains systèmes chromosomiques puissent conférer un avantage sélectif à leurs hôtes bactériens en interférant avec le PSK médié par leurs homologues plasmidiques. Cet avantage sélectif pourrait permettre la fixation de systèmes TA latéralement acquis au sein des populations bactériennes. Nous avons également recherché de nouveaux systèmes ccd au sein des génomes bactériens afin d’avoir un aperçu de leur distribution, des contextes génétiques dans lesquels ils existent et de l’implication du THG dans leur dispersion. Les réflexions qui ont accompagné notre recherche nous ont mené à proposer une synthèse sur le rôle des systèmes TA (plasmidiques et chromosomiques). Celle-ci se nourrit des avancées qui ont été effectuées, ces dernières années, dans la compréhension de l’évolution des génomes bactériens, de la théorie hiérarchique de la sélection naturelle et des processus non-adaptatifs et contingents qui pourraient expliquer la présence et la propagation des systèmes TA au sein des génomes bactériens sans que ceux-ci en soient les agents causaux.
Doctorat en sciences, Spécialisation biologie moléculaire
info:eu-repo/semantics/nonPublished
Kapadia, Jaimin Maheshbhai. "DNA transfer in the soil bacterium Rhodococcus." Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/honors/565.
Повний текст джерелаGASC, ROMEO ANNE-MARIE. "Etude de la specificite du systeme de reparation des mesappariements au cours de la transformation chez streptococcus pneumoniae." Toulouse 3, 1987. http://www.theses.fr/1987TOU30171.
Повний текст джерелаColomer, Lluch Marta. "Antibiotic resistance genes in the viral DNA fraction of environmental samples = Gens de resistència a antibiòtics en el DNA de la fracció vírica de mostres ambientals." Doctoral thesis, Universitat de Barcelona, 2014. http://hdl.handle.net/10803/144525.
Повний текст джерелаLa tesi doctoral que es presenta a continuació té com a objectiu principal l’estudi de gens de resistència a antibiòtics de rellevància clínica en la fracció de DNA de partícules de bacteriòfags aïllades de diferents tipus de mostres ambientals per tal de determinar la importància dels bacteriòfags com a vehicles de mobilització de gens de resistència a antibiòtics entre bacteris. S’ha estudiat un ampli espectre de gens de resistència a antibiòtics com a representants dels grups principals descrits actualment en la nostra àrea geogràfica corresponent a tres β-lactamases (blaTEM, blaCTXM-1 i blaCTX-M-9), el gen mecA de resistència a meticil•lina en estafilococs, i els gens de resistència a quinolones qnrA i qnrS. Per això s’han analitzat diversos tipus de mostres procedents d’aigua residual municipal, d’aigua de riu i d’aigua residual amb contaminació fecal animal per tal de quantificar els gens de resistència a antibiòtics d’interès en DNA aïllat de bacteriòfags. Durant els diferents estudis s’ha intentat optimitzar la metodologia d’extracció de DNA de bacteriòfags així com els controls corresponents per garantir l’amplificació de DNA encapsidat i l’eliminació de qualsevol DNA lliure present a les mostres i de qualsevol possible vesícula amb DNA al seu interior. Per altra banda, també s’ha determinat la capacitat funcional dels gens de resistència detectats en DNA de fags i per això s’han realitzat experiments de transformació a partir de soques bacterianes sensibles a un determinat antibiòtic amb l’objectiu d’incorporar la resistència i per tant, esdevenir resistents a l’antibiòtic en qüestió. També, s’ha estudiat la influència de determinats compostos implicats en la inducció del cicle lític de bacteriòfags temperats, en l’augment en el nombre de còpies de gens de resistència a antibiòtics en DNA present en la fracció de fags de l’aigua residual. Finalment, degut a la importància de la transferència horitzontal de gens com a mecanisme de dispersió de la resistència a antibiòtics en el medi ambient i en clínica s’han dut a terme experiments de transducció per tal d’intentar reproduir in vitro el procés que tindria lloc de manera natural.
Clavé, Corinne. "Flux ioniques et energetique cellulaire au cours de l'induction de la competence chez streptococcus pneumoniae, leur implication dans le transport de l'adn." Toulouse 3, 1988. http://www.theses.fr/1988TOU30063.
Повний текст джерелаAndrade, Alexander de. "Avaliação de parâmetros que influenciam a transformação genética do Eucalyptus grandis via Agrobacterium." Universidade de São Paulo, 2001. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-15032002-111342/.
Повний текст джерелаThe research project aimed the establishment of a method for genetic transformation of Eucalyptus grandis; an important and widely planted forestry tree in Brazil. Two techniques of transformation were tested: agrobiolistic of explants with accumulation of meristematic cells and SAAT ('sonication-assisted Agrobacterium mediated transformation'). Both systems of transformation aim to produce micro wounds in the explants in order to increase the Agrobacterium penetration in the tissues. In the case of agrolistica the explant was previously submitted to micro projectile bombardment, followed by bacteria inoculation. On the other hand in the SAAT technique the explante is submitted to short periods (few seconds) of sonication together with the bacteria. Transformation was evaluated by measuring the expression of the reporter gene uidA which codes the ß-glucuronidase enzyme. Several parameters were tested for agrobiolistic such as, gas pressure (helium), flight distance of micro projectiles, gene expression of the uidA, co-cultivation temperature. The highest values of ß-glucuronidase were observed for 1350 PSI, 9.5 cm from target and 26O C for co-cultivation. A histological analyze was carried out to check it the meristematic tissues were being transformed. The results showed that the meristematic tissues were localized in the surface of the explante. The meristem is formed by the dedifferentiation of epidermal cells and the first layers of the cortical parenchyma indicating its exogenous origin. The histological location of the reporter gene uidA showed that the expression occurs only in cells already differentiated, and not in meristematic cells. For SAAT experiments a clone was previously selected for favorable characteristic of transformation: high rate of regeneration, susceptibility of selective agents and to Agrobacterium transformation. The highest rate of ß-glucuronidase activity were observed for 60 s of sonication; higher sonication time reduced the efficiency of regeneration. Additional sonication, following a preliminary sonication of explante , in the presence of the bacteria, improved the efficiency of transformation. The results provided evidence the SAAT is a viable technique for Eucalyptus transformation via Agrobacterium.
Michel, Bénédicte. "Recombinaison homologue et illegitime chez bacillus subtilis et escherichia coli." Paris 6, 1986. http://www.theses.fr/1986PA066534.
Повний текст джерелаFelipe, Rafaella Teles Arantes. "Avaliação da resistência à Candidatus Liberibacter asiaticus em laranja doce expressando o gene attA ou hrpN." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-23032012-093114/.
Повний текст джерелаHaunglongbing (HLB), considered one of the most serious diseases of citrus, is associated to Candidatus Liberibacter spp., endogenous and phloem-inhabiting bacteria not easily grown in culture medium No species within the genus Citrus is known to resist this bacterial infection. The use of genes of agronomic interest for genetic transformation aiming disease resistance in citrus has been reported. Among these genes, attA that codes for the antibacterial peptides attacin, and hrpN, that codes for proteins harpin that activate the plant defense system may have potential in searching for HLB resistance. The objective of this study was to evaluate the resistance of sweet orange containing attA or hrpN to Candidatus Liberibacter asiaticus (CLas) inoculated through infected budstick grafting or the insect vector, Diaphorina citri. For the plants containing hrpN, only the second method was used. The most obvious HLB symptoms were observed four and eight months after inoculation by infected budstick when CLas also was detected by PCR (four months) and RT-qPCR (eight months). For those inoculated with D. citri, symptoms were observed and bacteria detected eight and twelve months after inoculation. Fifteen, 17 and 18 months after inoculation, a new attempt was made for CLas detection, now through Rt-PCR from leaf and psyllids imprinting spots on membrane. It was not possible to evaluate the HLB resistance in plants containing attA or hrpN gene from D. citri inoculation. The results of CLas detection in plants and psyllids indicate that possibly there was no inoculation due the low rate of psyllids contained CLas used. Among the plants containing attA, five, two and one event of, respectively, Pera, Hamlin, and Valencia sweet orange had lower bacterial titers than those non transgenic plants and some also showed milder HLB symptoms, eight months after inoculation, suggesting a possible effect of attacin A against the causal agent of HLB.
Centis, Sonia. "Transfert de fragments de genes d'un caulimovirus dans le colza (brassica napus l. ) : obtention de plantes transgeniques." Toulouse 3, 1988. http://www.theses.fr/1988TOU30198.
Повний текст джерелаLan, Jhih-Fan, and 藍志帆. "Studies on Resistance of Bacterial Soft Rot by Genetic Transformation in Phalaenopsis." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/38065793238456382862.
Повний текст джерела臺灣大學
園藝學研究所
95
The purpose of this study is to enhance the resistance of orchids to bacterial soft rot. Pectate lyase genes ( pelE and pelZ ) isolated from Erwinia chrysanthemi were transformed into calli of Phalaenopsis and leaves of tobacco by Agrobacterium-mediated transformation. Transformed calli were selected by medium containing G418 due to nptII selectable marker gene in pelE construct. PelE transgenic callus lines confirmed by molecular analysis were further transformed with pelZ construct containing hpt and gus genes. Therefore, survival transformed callus were double transformed after selection by hygromycin. After callus was selected with antibiotics and regenerated, transgenic lines were confirmed by β-glucuronidase ( GUS ) activity, polymerase chain reaction ( PCR ), and Southern blot analysis. Expression products of transgenes were detected 43 and 49 kD by Western blotting analysis and enzyme-linked immunosorbent assay ( ELISA ). Hydrogen peroxide staining and pathogen inoculation analysis assay revealed that transgenic lines exhibited hypersensitive response ( HR ) to phytopathogens.
Wyckoff, Herbert Allen 1961. "Development and use of genetic techniques for study of dairy Leuconostoc bacteria." Thesis, 1992. http://hdl.handle.net/1957/36485.
Повний текст джерелаErraguntla, Mythili. "Genetic Analysis And Biochemical Activities Of β Protein : A Component Of Bacteriophage λ General Genetic Recombination". Thesis, 1995. http://etd.iisc.ernet.in/handle/2005/1906.
Повний текст джерелаJang, Ki-Hyo. "Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species." Thesis, 1998. https://vuir.vu.edu.au/15345/.
Повний текст джерелаChen, Hsing-Ta, and 陳幸達. "Improving Genetic Stability by Utilizing Bacteria with Linear Transformation during Recombinant Protein Overexpression." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/ksdm78.
Повний текст джерела國立臺北科技大學
化學工程所
93
In this research, we use linear transformation that takes advantage of the homologous sequence between two ends of linear DNA and the host cell E.coli, to transfer the target genes directly into the chromosome of the strain. Because this method enables expressing recombinant proteins continuously without plasmid, the problem of plasmid instability is solved. In practice, we use ZSC114, a multi-auxotrophic E.coli strain that cannot metabolize glucose, mannose and lactose, as our host cell. First, we unite each of the three metabolism-related genes with lacZ gene, to prepare for a functional linear DNA. Then we put these linear substrates into the chromosome of ZSC114. We can make up for the auxotrophic phenotype of the original strain, and with expression of more lacZ genes, enhance the production of recombinant protein β-galactosidase. Protein gel electrophoresis andβ-galactosidase activity assay are executed to measure the production of recombinant protein LacZ among our newly constructed strains. We find out that the production and activity of the genetic product increase while the copy number of lacZ gene rises in the bacterial chromosome. We use the method of serial dilution culture to investigate genetic stability between the three-lacZ gene-carrying strain, ZSCGMtZ, and the plasmid expression system, ZSC114/pJL. We find out that in serial dilution culture, the ZSC114/pJL system would loss LacZ expression at 35th hour, but new strain ZSCGMtZ still keep quite high recombinant protein expression after 60 hours. The result indicates the genetic stability of new strain is better.