Дисертації з теми "Bacillus proteus"
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Contesini, Fabiano Jares. "Produção, caracterização e aplicação de proteases de Bacillus sp. = Production, characterization and application of proteases from Bacillus sp." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254357.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Proteases bacterianas são enzimas de elevada importância comercial, amplamente aplicadas em diversas áreas como nas indústrias de detergentes, de alimentos, farmacêutica e têxtil. Este trabalho teve como principais objetivos selecionar entre 59 linhagens de Bacillus sp., da coleção de culturas do Laboratório de Bioquímica de Alimentos da FEA, aquelas que apresentam potencial de maior produção de proteases com características tais como estabilidade em diferentes condições de temperatura, pH, detergentes e solventes orgânicos, atividade em ampla faixa de pH e capacidade de lisar células de Xanthomonas campestris. Em seguida, visou-se otimizar a produção de proteases pela linhagem selecionada, determinar as características bioquímicas da protease parcialmente purificada e estudar a aplicação do extrato enzimático bruto e preparação parcialmente purificada. Entre as cinquenta e nove linhagens de Bacillus sp. testadas foram selecionadas nove linhagens que produziram maior atividade de proteases. A produção de protease pelas nove linhagens foi testada em frascos agitados contendo o meio de cultura nº 1 (10g/L de caseína, 1g/L de extrato de levedura e sais), meio nº 2 (35 g/L de melaço de cana de açúcar, 20g/L de água de maceração de milho, 3g/L de extrato de levedura Prodex-Lac SD® e 20g/L de soro de queijo), e por fermentação em meio sólido nº 3 (farelo de trigo e água, na proporção 1:1, m:m). As linhagens de Bacillus sp. LBA 07, Bacillus sp. LBA 46 e Bacillus sp. LBA 08 fermentadas nos meios de cultura nº 1, nº 2 e nº 3 produziram 222 U/mL, 548 U/mL e 13480 U/grama de substrato seco (gss) respectivamente. As proteases dos extratos enzimáticos brutos obtidos das nove linhagens fermentadas nos três meios de cultura apresentaram atividade ótima na faixa de pH 7 a 9 e 60° C, estabilidade na faixa de pH 5 a 9 por 24h a 4º C , e após 1 h de tratamento a 50° C. Entre os extratos enzimáticos brutos de proteases testados, aqueles obtidas da fermentação de Bacillus sp. LBA 46 nos três meios de cultura foram as mais estáveis em detergente Ariel®. Quando incubadas em solventes orgânicos alguns extratos enzimáticos brutos proteases mantiveram mais de 60% de atividade residual após 24h em acetona (Bacillus sp. LBA 8 e 44), hexano (Bacillus sp. LBA 19, 29, 44, 46 e 60), clorofórmio (Bacillus sp. LBA 44 and 60) e etanol (Bacillus sp. LBA 60). Os extratos enzimáticos brutos de proteases obtidos do cultivo da linhagem de Bacillus sp. LBA 46 nos meios n° 2 e n° 3 foram as mais eficientes na lise de células de Xanthomonas campestris, aumentando cerca de 30% a transmitância a 620 nm (Trans 620nm) do meio fermentado de goma xantana. A linhagem de Bacillus sp. LBA 46 foi selecionada como melhor produtora de protease e estudos preliminares de identificação biomolecular indicam que se trata de uma linhagem de Bacillus licheniformis. Utilizando-se a linhagem de Bacillus sp LBA 46 e o meio de cultura otimizado (meio n° 4) por metodologia de superfície de resposta (MSR), composto de 40g/L de melaço de cana de açúcar, 6g/L de água de maceração de milho, 2g/L de extrato de levedura Prodex-Lac SD® e 20g/L de soro de queijo, foi obtido 3000 U/mL de protease após 96h de fermentação a 30° C e 200 rpm. No estudo da aplicação da enzima para a remoção de manchas de tecidos de algodão foram obtidos melhores resultados de remoção de manchas de sangue e molho de tomate com carne moída, utilizando-se a combinação de extrato bruto de protease (100 ou 1000U) com o detergente Omo®. O extrato enzimático bruto da linhagem de Bacillus sp. LBA 46 foi parcialmente purificado por fracionamento com sulfato de amônio (80% de saturação), diálise e cromatografia de filtração em gel (Sephadex G100), resultando em fator de purificação de 3,69. Após caracterização com MSR observou-se que a protease da preparação parcialmente purificada apresentou atividade ótima a 55° C e pH 7,5 e considerável estabilidade (95% de atividade residual) na faixa de pH 5,7 ¿ 9,3 após 1h de incubação a 30 ¿ 36° C, e acima de 78,9% quando incubadas por 1h em pH 7,5 e 50° C. A condição ótima de lise das células de X. campestris do meio fermentado de goma xantana utilizando-se o extrato enzimático bruto de protease e a preparação parcialmente purificada de proteases, foi observada utilizando 42 U de protease /mL de suspensão celular de X. campestris a 60° C, resultando em aumento de mais de 20% da Trans 620nm do meio fermentado de goma xantana. Um aumento de quase 40% de Trans 600nm foi observado após 2h de reação utilizando extrato enzimático bruto de protease (42 U de protease/mL de suspensão celular de X. campestris) a 65° C. A produção de proteases de Bacillus sp. LBA 46 por fermentação em estado sólido foi otimizada utilizando MSR, sendo obtido 5000 U/grama de substrato seco utilizando-se meio de cultura composto de farelo de trigo e água (60%:40%) após 96h de fermentação a 30° C
Abstract: Proteases are commercially relevant enzymes widely applied in several industrial areas, such as in detergent, food, pharmaceutical and textile industries. Proteases from Bacillus sp. can present advantages compared to the proteases from other sources, including better thermostability, stability in pH range from slightly acid to alkaline pH values and stability in organic solvents. The aims of this work were selecting Bacillus sp. strains with capability of producing proteases with better biochemical properties, such as stability in different conditions of temperature, pH, detergents and organic solvents, activity in a wide range of pH and capability of lysing cells of Xanthomonas campestris. Afterwards, it was aimed the optimization of the production of proteases by the selected Bacillus sp. strain and the determination of the biochemical characteristics of the partially purified protease and the application of the crude and partially purified protease. Nine Bacillus sp. strains were selected as the best protease producers among fifty nine Bacillus sp. strains tested. The protease production by the nine strains was carried out in Erlenmeyer flasks containing medium no. 1 (10g /L of casein, 1g/L of yeast extract and salts), medium no. 2 (35 g/L of sugar cane molasses, 20g/L corn steep liquor, 3g/L of yeast extract Prodex-Lac SD® and 20g/L of dried whey), e by fermentation using solid substrate medium no. 3 (wheat bran and water, 1:1, m:m). The strains Bacillus sp. LBA 07, Bacillus sp. LBA 46 and Bacillus sp. LBA 08 when fermented in medium no. 1, no. 2 e no. 3 produced 222 U/mL, 545 U/mL and 13480 U/gram of dried substrate (gds) respectively. Proteases from the crude enzymatic extracts obtained from the fermentation of the nine Bacillus sp. strains in the three media showed optimal activity in pH range 7-9 and 60° C, stability in pH range 5-9 for 24 hours at 4° C and after 1h at 50° C. The protease preparations from the fermentation of Bacillus sp. LBA 46 in the three media were the most stable when incubated in detergent Ariel®, among the proteases tested from the Bacillus sp. strains. In addition, some proteases presented more than 60% residual activity after 24h in the organic solvents acetone (Bacillus sp. LBA 8 and 44), hexane (Bacillus sp. LBA 19, 29, 44, 46 and 60), chloroform (Bacillus sp. LBA 44 and 60) and ethanol (Bacillus sp. LBA 60). The protease preparations obtained from the cultivation of Bacillus sp. LBA 46 in medium no. 2 and no. 3 presented the best results on the lysis of Xanthomonas campestris cells, resulting in an increase of approximately 30% in transmittance at 620 nm (Trans 620nm) of the fermented broth of xanthan. Bacillus sp. LBA 46 strain was selected as the best protease producer and after preliminary biomolecular analysis of identification, the results indicate that this microorganism correspond to a Bacillus licheniformis strain. Protease preparation containing 3000 U/mL was obtained from Bacillus sp. LBA 46 cultivated in Erlenmeyer flasks containing medium no. 4 composed of 40g/L of sugar cane molasses, 6g/L of corn steep liquor, 2g/L of yeast extract Prodex-Lac SD® and 20 g/L of dried whey after 96h of fermentation at 30° C and 200 rpm, optimized with response surface methodology (RSM). In the the washing tests, the best results of the removal of blood and tomato sauce with ground beef stains from cotton fabrics were observed using the combination of crude extract of protease (100 or 1000U) with detergent Omo®. Crude protease extract of the Bacillus sp. LBA strain was partially purified by ammonium sulfate fractionation (80% saturation), dialysis and gel filtration chromatography (Sephadex G100), resulting in the purification fold of 3.69. After characterization with RSM it was observed that the crude protease extract and partially purified proteases presented optimal activity at 55° C and pH 7.5 and considerable stability (95% of residual activity) in pH range 5.7 ¿ 9.3 after 1h incubation at 30-36° C and more than 78.9% when incubated at pH 7.5 and 50 °C for 1h. The optimal conditions of the lysis of X. campestris cells contained in the fermentation broth using crude and partially purified protease preparations were observed using 42 U of protease/mL of cell suspension of X. campestris at 60° C, resulting in a increase of more than 20% in Trans 620 nm of the fermented broth of xanthan. It was observed an increase of almost 40% in Trans 620 nm after 2h reaction using crude protease (42 U de protease/mL of cell suspension of X. campestris) at 65° C. The production of proteases by Bacillus sp. LBA 46 under solid state fermentation was optimized using RSM, resulting in 5000 U/gram of dry substrate utilizing wheat bran and water (6g:4g) after 96h of fermentation at 30° C
Doutorado
Ciência de Alimentos
Doutor em Ciência de Alimentos
McNeil, Betina C. "Mutational Analysis and Characterization of Microbial Pesticides Isolated from Bacillus Thuringiensis." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316527600.
Повний текст джерелаCarmel, Andrew Barry. "Crystal structure of BstDEAD, a novel DEAD-box protein from Bacillus stearothermophilus /." view abstract or download file of text, 2003. http://wwwlib.umi.com/cr/uoregon/fullcit?p3095239.
Повний текст джерелаTypescript. Includes vita and abstract. Includes bibliographical references (leaves 101-114). Also available for download via the World Wide Web; free to University of Oregon users.
Thwaite, Joanne E. "Factors influencing the production of Bacillus anthracis protective antigen in Bacillus subtilis." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369784.
Повний текст джерелаWalker, Dianne. "Bacillus stearothermophilus pyruvate kinase." Thesis, University of Bristol, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335572.
Повний текст джерелаTruong, Hung Phuc. "Fate of Cry Toxins from Bacillus thuringiensis in soil." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS210.
Повний текст джерелаThe insecticidal properties of Bacillus thuringiensis, discovered by Shigentane Ishiwatari, have been used for decades as biopesticides and this use has been increasing rapidly because of concerns about the negative environmental effects of chemical pesticides. Currently, Bt toxin in the form of both biopesticides and Bt transgenic plantsmay supplement or replace chemical pesticide. There is little evidence to demonstrate that Bt toxin has any harmful effect to the environment or to human health. Nevertheless, there are concerns that commercial transgenic crops may have harmful impacts on the environment. After release into soil via root exudation and breakdown of plant residues, Bt toxin interacts with soil particles. The interactions of Bt toxin with soil particles influence its mobility, its bioavailability, its persistence and its toxicity. In this study, we aim to establish the relative importance of biological and physicochemical factors in the determination of the dynamics of detectable Cry proteins in soils, to clarify if adsorbed protein maintains its insecticidal properties and to identify the soil properties that determine the fate of Cry proteins in soil. The results show that Cry proteins have strong affinity on soil surface. However, there was little relationship between affinity for soil or the extraction yield and soil properties including clay content, organic carbon content and soil pH. There was little relationship between the affinity and the extraction yield. The proteins differ in both their affinity for soil and their extraction yields.An assessment of role of soil and environmental factors in the fate of Cry protein from commercial biopesticide formulation showed a rapid decline of detectable Cry protein subjected to direct sunlight under the laboratory condition, whereas, little effect was observed under field conditions. The half-life of proteins in soil under natural conditions was about one week. Strong temperature effects were observed, but theydiffered for biopesticide and purified protein, indicating different limiting steps. For biopesticide, the observed decline was due to biological factors, possibly including sporulation. In contrast for purified proteins, increased temperature enhanced conformationalchanges of the soil-adsorbed protein, leading to fixation and hence extraction efficiency decreased that decreased with time. Moreover, the study of persistence of various Cry proteins in contrasting soils was carried out by immuno-detection and bioassay showed that extractable toxin decreased with incubation of up to four weeks. Insecticidal activity was still retained in the adsorbed state, but lost after two weeks of incubation at 25°C. The decline in extractable protein and toxicity was much lower at 4°C than 25°C. There was no significant effect of soil sterilization to persistence of Cry toxin indicating that decrease in detectable Cry toxin in soil may be time-dependent fixation of adsorbed protein as well as decreasing solubilization in larva midgut, but not microbial breakdown.Exposition to Cry in the adsorbed form could have a significant impact on target and even non target insects and should be investigation to determine the potential impact
Shakir, Salika Mehreen. "Characterization of a serine/threonine phosphatase-kinase pair in Bacillus anthracis." Oklahoma City : [s.n.], 2010.
Знайти повний текст джерелаDürrschmidt, Peter. "Entfaltung und Autoproteolyse der neutralen Protease aus Bacillus stearothermophilus und einer Disulfid-modifizierten Variante." [S.l. : s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970184883.
Повний текст джерелаXue, Yong. "Effects of Protein Domains on Localization of Penicillin-Binding Proteins 2a and 2b in Bacillus Subtilis." Thesis, Virginia Tech, 2008. http://hdl.handle.net/10919/35255.
Повний текст джерелаMaster of Science
Rawlings, Andrea Elizabeth. "Structural investigations of proteins from Bacillus." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489200.
Повний текст джерелаStaples, Nicholas James. "Investigation of Bacillus thuringiensis 'helper' proteins." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627245.
Повний текст джерелаNair, Manoj S. "Mechanism of action of insecticidal crystal toxins from Bacillus thuringiensis biophysical and biochemical analyses of the insertion of Cry1A toxins into insect midgut membranes /." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1218558470.
Повний текст джерелаSayer, Cameron Vincent. "Identification and Analysis of Germination-Active Proteins in Bacillus Spores." Diss., Virginia Tech, 2019. http://hdl.handle.net/10919/90885.
Повний текст джерелаDoctor of Philosophy
Few bacterial species can undergo a specialized division process leading to the generation of a bacterial endospore. Endospores are dormant cells that boast resistance to a variety of environmental conditions that would otherwise cause bacterial cell death. These resistance traits make endospores immune to traditional bactericidal methods, making decontamination a nontrivial task. Further complicating the matter, spores are often the infectious particle of the associated disease, including hospital acquired diarrhea, infant botulism, anthrax, and many others. Presented work focuses on furthering understanding the process by which a dormant spore returns to a typical growing bacteria cell. Comprehension of major steps in this process may lead to novel methods for spore cleanup in which mechanisms within the spore are subverted to force a return to a typical bacterial cell state.
Rueff, Anne-Stéphanie. "Role de protéines associées au cytosquelette bactérien." Phd thesis, Université Paris Sud - Paris XI, 2011. http://tel.archives-ouvertes.fr/tel-00633025.
Повний текст джерелаAbdullah, Mohd Amir F. "CHARACTERIZATION OF TOXICITY DETERMINANTS IN BACILLUS THURINGIENSIS MOSQUITOCIDAL DELTA-ENDOTOXINS." The Ohio State University, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=osu1039312355.
Повний текст джерелаKorkmaz, Nuriye. "Recombinant Therapeutic Protease Production By Bacillus Sp." Master's thesis, METU, 2007. http://etd.lib.metu.edu.tr/upload/3/12608656/index.pdf.
Повний текст джерелаend of the streptokinase gene (skc: Acc. No. S46536) by SOE (Gene Splicing by Overlap Extension) method through PCR. The resulting hybrid gene pre(subC)::skc was cloned into the pUC19 plasmid. Then, the hybrid gene was sub-cloned to pMK4 plasmid which is an E. coli-Bacillus shuttle vector with high copy number and high stability. Recombinant plasmid pMK4::pre(subC)::skc was finally transferred into B. subtilis (npr- apr-) and B. licheniformis 749/C (ATCC 25972) species. Streptokinase production capacities of these two recombinant Bacillus species were compared. The highest production was observed in recombinant B. lichenifomis 749/C (ATCC 25972) strain in a defined medium which was optimized in terms of carbon and nitrogen sources by a statistical approach, namely Response Surface Methodology (RSM). RSM evaluated the streptokinase concentration as the response and the medium components as the independent variables. The highest recombinant streptokinase concentration was found as 0.0237 kgm-3 at glucose and (NH4)2HPO4 concentrations of 4.530 and 4.838 kgm-3 respectively. The fermentation and oxygen transfer characteristics of the streptokinase production were investigated in a 3 dm3 pilot scale batch bioreactor (Braun CT2-2) equipped with temperature, pH, foam, air inlet and agitation rate controls having a working volume of VR=1.65 dm3 using the production medium optimized for the recombinant B. lichenifomis 749/C (ATCC 25972) strain. Streptokinase and &
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-lactamase activities, cell, glucose and organic acid concentrations, dissolved oxygen, pH, oxygen uptake rate, overall liquid phase mass transfer coefficient for oxygen, maintenance coefficient for oxygen, specific cell growth rate and yield coefficients were determined through the bioprocess. The bioprocess of recombinant streptokinase production was performed at uncontrolled pH of these bioreactor operation conditions: air inlet rate of Q0/VR=0.5 vvm, and the agitation rate of N=400min-1. The resulting streptokinase volumetric activity reached its maximum as 1.16 PUml-1 (0.0026 g/l streptokinase) at t=20 h.
Wolf, Diana. "Das Phagenschock-Protein LiaH aus Bacillus subtilis." Diss., lmu, 2012. http://nbn-resolving.de/urn:nbn:de:bvb:19-151553.
Повний текст джерелаCoxon, Rosemary D. "Factors affecting protein export from Bacillus subtilis." Thesis, University of Newcastle Upon Tyne, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287424.
Повний текст джерелаSeavers, Philippa Ruth. "Structural studies of the Bacillus SpoIIA proteins." Thesis, University of York, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369297.
Повний текст джерелаWan, Qiang. "Structure and assembly of Bacillus spore proteins." Thesis, University of Sheffield, 2013. http://etheses.whiterose.ac.uk/4161/.
Повний текст джерелаPrajapati, Rohit. "Analysis of the Bacillus subtils sporulation protein SpoVM and its interaction with the multifunctional protease FtsH." Thesis, Royal Holloway, University of London, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405110.
Повний текст джерелаGlatz, Elisabeth. "Bacillus subtilis GlpP protein, antitermination and mRNA stability." Lund : Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/68945078.html.
Повний текст джерелаFürch, Tobias. "Metabolic characterization and optimization of recombinant protein production in Bacillus megaterium." Paderborn FIT-Verl. für Innovation und Technologietransfer, 2008. http://d-nb.info/991888510/04.
Повний текст джерелаChen, Yan. "Characterization of Bacillus Spore Membrane Proteomes and Investigation of Their Roles in the Spore Germination Process." Diss., Virginia Tech, 2014. http://hdl.handle.net/10919/64934.
Повний текст джерелаPh. D.
Yssel, Anna Elizabeth Johanna. "The spatial evolution of the chemotaxis proteins of the Bacillus subtilis group." Thesis, Rhodes University, 2011. http://hdl.handle.net/10962/d1004087.
Повний текст джерелаHaider, Syed Tanveer. "Host-range specificity of a Bacillus thuringiensis (Bt) toxin as analyzed by protein engineering /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/5269.
Повний текст джерелаJohnson, Christian Lloyd. "Proteins involved in the germination of bacillus spores." Thesis, University of Sheffield, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.425623.
Повний текст джерелаGençkal, Hande Tarı Canan. "Studies On Alkaline Protease Production From Bacillus Sp./." [s.l.]: [s.n.], 2004. http://library.iyte.edu.tr/tezler/master/biyoteknoloji/T000505.pdf.
Повний текст джерелаKoya, Vijay. "EVALUATION OF IMMUNOGENICITY OF TRANSGENIC CHLOROPLAST DERIVED PROTECT." Master's thesis, University of Central Florida, 2004. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3023.
Повний текст джерелаM.S.
Department of Molecular Biology and Microbiology
Burnett College of Biomedical Sciences
Molecular Biology and Microbiology
Veltman, Oene Robert. "Engineering high performance variants of Bacillusthermolysin-like proteases." [S.l. : [Groningen] : s.n.] ; [University Library Groningen] [Host], 1997. http://irs.ub.rug.nl/ppn/164267484.
Повний текст джерелаKuo, Shrin Paul. "Characterization of the Bacillus subtilis protein Obg : a dissertation /." San Antonio : UTHSC, 2007. http://proquest.umi.com/pqdweb?did=1428842521&sid=1&Fmt=2&clientId=70986&RQT=309&VName=PQD.
Повний текст джерелаScotney, Pierre David. "The catalytic mechanism of Bacillus stearothermophilus pyruvate kinase." Thesis, University of Bristol, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266959.
Повний текст джерелаOzcelik, Hayriye. "Productivity Analyses In Fermentations With Three Different Biolarvacides." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12604988/index.pdf.
Повний текст джерелаKrauss, Oliver. "Structural studies on glycerol dehydrogenase from Bacillus stearothermophilus." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243048.
Повний текст джерелаChoma, Christin Teresa. "Structural characterization of the insecticidal protein from Bacillus thuringiensis." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5624.
Повний текст джерелаBietlot, Henri P. "Characterization of the insecticidal crystal protein from Bacillus thuringiensis." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5668.
Повний текст джерелаDrechsel, Susan. "Struktur- und Funktionsanalyse der Protease RasP aus Bacillus subtilis." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-152245.
Повний текст джерелаNgo, Thi Hoa. "Characterisation of the bacillus subtilis intercompartmental signalling protein, SPOIVB." Thesis, Royal Holloway, University of London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395611.
Повний текст джерелаKihlken, Margaret A. "Studies of predicted copper trafficking proteins from Bacillus subtilis." Thesis, University of East Anglia, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.405395.
Повний текст джерелаMongkolthanaruk, Wiyada. "Functional analysis of spore germination proteins of Bacillus subtilis." Thesis, University of Sheffield, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.444580.
Повний текст джерелаLeejeerajumnean, Suporn. "Studies of a 'Bacillus subtilis' multifunctional regulatory protein sinR." Thesis, University of York, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.428469.
Повний текст джерелаAhmad, Wasim. "Genetics and biochemistry of Bacillus thuringiensis insecticidal protein [?]-endotoxin." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306309.
Повний текст джерелаKouwen, Thijs R. H. M. "Protein secretion and disulfide bond handling in bacillus subtilis." [S.l. : [Groningen : s.n.] ; University Library Groningen] [Host], 2009. http://irs.ub.rug.nl/ppn/315686960.
Повний текст джерелаBarat, Bidisha. "Characterization of proteins involved in Bacillus subtilis spore formation and germination." Diss., Virginia Tech, 2020. http://hdl.handle.net/10919/98541.
Повний текст джерелаDoctor of Philosophy
Bacillus subtilis is an ubiquitous bacterium that is capable of forming endospores when faced with unfavorable environmental conditions. Spores are highly resistant to heat, radiation, lack of nutrients, desiccation and oxygen deprivation. They lack metabolism, which effectively keeps them in a state of suspended animation until germinated. They may remain stable and viable in this state for extremely long periods of time. Several important pathogenic bacteria are spore formers. This leads to difficulty in their environmental eradication and the treatment of patients. Germination allows spores to resume metabolism and reestablish a vegetative state. Certain key molecules activate the germination process. Each species of spore-forming bacteria has a specific set of these molecules called germinants that will enable the spore to exit its dormant state. The work presented focuses on the understanding of the germination apparatus of Bacillus subtilis, which may provide a model to understand the germination of other spore formers and help to improve methods of decontamination.
Seixas, Ana Claudia Mendes de. "Produção e utilização de protease de Bacillus subtilis em tratamento de efluentes liquidos." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/255462.
Повний текст джерелаTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
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Resumo: Consideráveis pesquisas têm sido conduzidas nos últimos anos para descobrir novas enzimas que possam ser utilizadas no tratamento de resíduos. Devido ao aumento de poluentes no meio ambiente, tornando-se difícil a sua remoção por processos convencionais, o desenvolvimento de pesquisas com enzimas busca um processo alternativo de tratamento, de menor custo, mais rápido, mais simples e mais confiável. As proteases são a classe mais importante das enzimas industriais e compreendem cerca de 25% de todas as enzimas comerciais no mundo. A maior aplicação destas enzimas é nas indústrias de alimentos, farmacêuticas e de detergentes. A finalidade do presente trabalho consistiu num estudo para determinar as melhores condições para produção de protease, bem como sua eficiência na hidrólise enzimática sobre a etapa biológica no tratamento de efluente líquido. Para a produção da protease foi testada uma cepa de Bacillus subtilis. O inóculo foi preparado em frascos Erlenmeyers (250 mL) contendo 50 mL de meio BHI , previamente esterilizado (121ºC, 15 min). Os frascos foram incubados a 30ºC durante 24 h com agitação de 200 rpm. Para a produção de protease foi utilizado um Planejamento Experimental Fatorial Fracionário 24-1, com triplicata no ponto central, tendose como variáveis independentes as concentrações de lactose, cloreto de sódio, caseína e pH. Como base foi utilizado o meio descrito por KEMBHAVI et al. (1993). Baseado nos resultados do planejamento experimental, foi realizado um ensaio cinético em fermentador do tipo tanque agitado contendo lactose (5,0 g.L-1) e caseína (5,0 g.L-1) a pH 6, 30OC e 300 rpm durante 30 h. O meio foi inoculado numa proporção de 10% (v/v) de inóculo. As amostras foram retiradas em intervalos de 1h, centrifugadas e o sobrenadante armazenado para análise. A atividade da protease foi medida pelo Método de KUNITZ (1947), utilizando caseína como substrato. A produção de 1124 U. mL-1 de atividade proteolítica foi obtida após 24 horas de fermentação. Com o objetivo de utilizar os próprios rejeitos industriais disponíveis em grande quantidade e sem custo como fonte de nitrogênio para o enriquecimento do meio de cultivo foi empregada água residuária do abatedouro de aves Pena Branca suplementada com caseína e MgSO4 na produção da enzima. O emprego do extrato enzimático, contendo protease, em uma das etapas do tratamento de efluente foi avaliado em um sistema de lodo ativado em reator tipo batelada, variando-se a água residuária e o lodo ativado. Nos primeiros ensaios foram colocados nos reatores o lodo proveniente de um reator do abatedouro de aves Pena Branca, o efluente bruto, a cultura de Bacillus subtilis ou o extrato enzimático. Em ensaios posteriores utilizou-se o lodo aclimatado, água residuária sintética, a cultura de Bacillus subtilis ou o extrato enzimático. Comparando os resultados dos tratamentos das águas residuárias do abatedouro de aves Pena Branca e sintética, as taxas de remoção de DQO da água residuária sintética foram em média 4 vezes maiores do que as taxas de remoção de DQO da água residuária do abatedouro de aves Pena Branca. A atividade do extrato enzimático B utilizado na água residuária sintética foi maior do que a do extrato enzimático produzido pela água residuária do abatedouro de aves Pena Branca, 1.124 U.mL-1 e 730 U.mL-1, respectivamente
Abstract: Considerable researches were carried out in the last years to discover new enzymes for treatment of residues. Due to the increase of pollutants in the environment, becoming difficult its removal for conventional processes, the development of these researches seek a alternative treatment process, lower cost, faster, simpler and more trustworthy. Proteases are the most important class of industrial enzymes and comprise about 25% of commercial enzymes on the world. The major applications of these enzymes are in the food, pharmaceutical and detergent industries. The purpose of this work consisted of a study to determine the best conditions for production of protease, as well as its efficiency in enzymatic hydrolysis on the biological stage in the liquid effluent treatment. Bacillus subtilis was tested for protease production. Inoculum was prepared in 250-mL Erlenmeyer flasks containing 50 mL BHI strain, previously sterilized (121ºC, 15 minutes). The Erlenmeyer flasks were incubated at 30ºC for 24 h under agitation at 200 rpm. A Fractionary Factorial Experimental Planning was carried out in 24-1 with triplicate in the central point for protease production, independent variables were lactose, sodium chloride and casein concentration and pH. Strain described by KEMBHAVI et al (1993) was used as basis medium. Based in the results of the experimental planning a kinetic trial was carried out in fermentor type agitated tank containing lactose (5.0 g/L), casein (5.0 g/L), pH 6, 30ºC and 300 rpm for 30 h. The medium was inoculated in 10% (v/v) of inoculum proportion. The samples were removed in intervals of 1h, centrifuged and the supernatant stored for analysis. Protease activity was measured by the method of KUNITZ (1947) using casein as substrate. The production of 1,124 Um.L-1 proteolytic activity was obtained after 24 h of fermentation. With the objective to use the proper industrial wastes available in great amount and no cost as nitrogen source for the enrichment of the culture medium were carried out using wastewater from poultry slaughter Pena Branca supplemented with casein and MgSO4 for enzyme production. Using the enzymatic extract, contained protease, in one of the stages of the effluent treatment was evaluated in a batch reactor with activated sludge, varying the wastewater and the activated sludge. In the first assays were placed into the reactors the sludge (from poultry slaugther Pena Branca reactor), the crude effluent, Bacillus subtilis strain or enzymatic extract. Posterior assays were carried out with acclimatized sludge, synthetic wastewater, Bacillus subtilis strain or enzymatic extract. Comparing the results of the wastewater from poultry slaughter Pena Branca and synthetic wastewater treatments, the COD removal rates from synthetic wastewater were 4-fold higher than the wastewater from poultry slaughter Pena Branca. The enzymatic activity of B extract used in the synthetic wastewater was higher than the enzymatic extract produced by wastewater from poultry slaughter Pena Branca, 1,124 U.mL-1 and 730 U.mL-1, respectively
Doutorado
Doutor em Engenharia de Alimentos
Henriques, Gabriela. "Towards the understanding of the function and regulation of a membrane protein complex involving SppA and YteJ in Bacillus subtilis." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS191.
Повний текст джерелаWe have identified a membrane protein complex of Bacillus subtilis involving an unknown protein, YteJ, and SppA, a membrane protein first described as a signal peptide peptidase and later shown to be also involved in the resistance to antibacterial peptides of the lantibiotic family. Using deletion mutant strains, we showed that both proteins are involved in this resistance. In the ΔsppA strain, the ectopic overexpression of SppA not only restored the resistance, it also induced the formation of elongated cells, a phenotype suppressed by the simultaneous overexpression of YteJ. Furthermore, the expression of truncated versions of YteJ pinpointed the inhibitory role of a specific domain of YteJ. Finally, in vitro biochemical studies showed that SppA protease activity was strongly reduced by the presence of YteJ, supporting the hypothesis of an inhibition by YteJ. Our in vivo and in vitro studies showed that YteJ, via one of its domain, acts as a negative regulator of the protease activity of SppA in this complex. In conclusion, we have shown that SppA/YteJ complex is involved in lantibiotic resistance through the protease activity of SppA, which is regulated by YteJ
Hearne, Catherine Mary. "A study of the heat shock response of Bacillus subtilis." Thesis, University of Cambridge, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334111.
Повний текст джерелаPenninga, Dirk. "Protein engineering of cyclodextrin glycosyltransferase from Bacillus circulans strain 251." [S.l. : s.n.], 1996. http://irs.ub.rug.nl/ppn/153174293.
Повний текст джерелаReeves, Adam J. "Signaling and interaction of the Bacillus subtilis physical stress pathway regulators of sigma B : a dissertation /." San Antonio : UTHSC, 2007. http://proquest.umi.com/pqdweb?did=1390290691&sid=1&Fmt=2&clientId=70986&RQT=309&VName=PQD.
Повний текст джерелаNeumüller, Andrea. "Untersuchungen zur Bacitracin-Selbst-Resistenz in Bacillus licheniformis ATCC 10716 und Grundlagen zur gerichteten Protein-Evolution von Adenylierungs-Domänen aus Peptidsynthetasen." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=96238707X.
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