Дисертації з теми "Avian influenza virus M2e protein"
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Tam, Ho-man Alex. "Mechanisms underlying the hyper-induction of tumour necrosis factor alpha (TNF-? by avian influenza virus in human macrophages." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B41508300.
Повний текст джерелаTam, Ho-man Alex, та 譚浩文. "Mechanisms underlying the hyper-induction of tumour necrosis factor alpha (TNF-α) by avian influenza virus in human macrophages". Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41508300.
Повний текст джерелаHasan, Noor Haliza. "Avian Influenza virus M2e protein: Epitope mapping, competitive ELISA and phage displayed scFv for DIVA in H5N1 serosurveillance." Thesis, 2017. http://hdl.handle.net/2440/119643.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Animal and Veterinary Sciences, 2017
陳羽鴻. "Development of the M2 protein of H1N1 avian influenza virus subunit vaccine." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/bm6f8f.
Повний текст джерела國立嘉義大學
生物農業科技學系研究所
106
Avian influenza(Avian Influenza;AI)is commonly known as avian flu. It is one kind of animal infectious diseases caused by flu viruses. AI viruses can be grouped into highly pathogenic avian influenza (HPAI) and low pathogenic avian influenza (LPAI). The mortality of the poultry infected by HPAI can be higher than 80%. In this study, H1N1 viral M2 gene segment was constructed into the E. coli protein expression vector for expressing the M2 gene 's fusion protein with 8X-Histidine tag. The serum samples of vaccinated mice were assayed by dot blotting and ELISA. The specific antibody against the overexpressed M2 gene's protein produced in the vaccinated mice was determined by dot blotting assay. The M2 gene's fusion protein was also shown to have better immune response in association with the commercial adjuvant. These studies have laid a strong foundation for developing an effective AI subunit vaccine.
Vuong, Christine. "Evaluation of Sindbis-M2e Virus Vector as a Universal Influenza A Vaccine." Thesis, 2012. http://hdl.handle.net/1969.1/ETD-TAMU-2012-08-11706.
Повний текст джерелаChen, Chien-Hao, and 陳建豪. "Expression and application of avian influenza virus hemagglutinin protein." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/24673044416928532096.
Повний текст джерела國立臺灣大學
獸醫學研究所
94
From 1986 to 2003, the avian influenza viruses (AIV) isolated in Taiwan were all low pathogenic. Some low pathogenic H5N2 AIVs were isolated in Taiwan in 2004, resulting in stamping 380 thousands chickens out from 22 poultry flocks. To test if the virus titers increased during passaging, H5N2 and H6N1 isolates were passaged in specific-pathogen-free embryonated eggs. After 45 passages, the virus titer of H5N2 increased 100 times, and two amino acid residues in HA1 of H5N2 virus were changed. The partial fragments of HA1 relating to neutralizing epitopes of the H5N2 and the H6N1 isolates were cloned in pGEX-5X-1 vector (prokaryotic expression) and pcDNA3.1 vector (eukaryotic expression) for expression. The purified recombinant proteins were intramuscualarly injected in 4-week-old SPF chickens for two times. Two weeks after the second immunization, the serum from H6-immunized chickens showed hemagglutination inhibition (HI) titers of 24 to 26, but no antibody was detected in H5-immunized chickens. To test the increase efficiency of liposome compounds on vaccine, Newcastle disease vaccines coated with liposome and with liposome/chitosan were used to immunize chickens. The results showed that Newcastle disease vaccine coated with liposome/chitosan increased HI titers in vaccinated chickens but not vaccine coated with lipsome alone.
Luo, Yu-Li, and 羅昱立. "Studies of avian influenza virus-induced apoptosis and HA protein expression." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/28070314762861980293.
Повний текст джерела國立屏東科技大學
生物科技研究所
94
Avian influenza virus(AIV)belongs to orthomyxoviridae family. Highly pathogenetic avian influenza virus causes flowl plague in chickens and turkeys. The HA protein of AIV 1209 strain was expressed by Pichia pastoris yeast expression system. The results indicated that the tagged fusion protein was present both in medium and pellet. The HA protein could be cleaved into HA1(47 kDa) and HA2(22 kDa). The AIV CE9 could induce apoptosis in MDCK cell. To further study the molecular mechanism of AIV-induced apoptosis, the NA protein of AIV H5N2 CE9 strain was cloned into pcDNA3.1(-), and the recombinant DNA transfected into MDCK cell lines. The results indicated that at the same focus, caspase 3 were not detected in the MDCK cell expressing the GFP-NA fusion protein. Phylogenesis analysis of HA and NA gene of strains Taiwanese 1209 and CE9 was done to sequence with other H5N2 viruses in the world using MEGA 3.1 software. The result indicate the 1209 and CE9 strain belong to the same group as the Italy strain.
Singh, Shailbala. "First Characterization of Avian Memory T Lymphocyte Responses to Avian Influenza Virus Proteins." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7534.
Повний текст джерелаChien, Pin-Cheng, and 錢品澄. "Characterization of Monoclonal Antibodies against Nonstructural Protein 1 (NS1) of Avian Influenza Virus." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/43455856100074850441.
Повний текст джерела國立臺灣大學
獸醫學研究所
102
NS1 is an indictor Ag of influenza virus (IV) infection and it’s only produced during IV replication in very early stage of infection. It can be used in determining whether the chicken was infected or not, which also can be applied on early diagnosis of IV infection. Therefore, my study aimed to characterize the anti-NS1 MAb (αNS1 MAb) by indirect ELISA (iELISA), indirect immunofluorescence assay (IFA), western blotting (WB), immunohistochemistry stain (IHC) and mapping the antibody binding site by eukaryotic expression system (EES). Subsequently, to develop a rapid, sensitive and specific diagnostic MAbs-based NS1 Ag sandwich ELISA that might be incorporated with NP and M Ag ELISA kit for detecting the IV infection. The parental hybridoma have been prepared by immunizing Balb/c mice with E. coli expressed recombinant nonstructural protein 1 (rNS1) oringinated from IV isolates of A/chicken/Taiwan/2838V/00 (H6N1/2838). 16 αNS1 MAbs have been characterized by WB, IFA and iELISA with NS1 Ag from several IV isolates such as A/chicken/Miaoli/2904/00 (H6N1/2904), A/chicken/Taiwan/1209/03 (H5N2/1209). Meanwhile, the epitope mapping was studied in EES. Expression of full length rNS1 (residues 1-230) were all positive and 4 αNS1 MAb (4M4, 4M6, 4N5, 4R3) were negative in C-terminal deletion (residues 1-207). Accroding to epitope mapping analysis results, which can divide 16 αNS1 MAb into four groups (A, B, C &; D). The predictive epitopes of 16 αNS1 MAb mainly recognize the effector domain (residues 74-230) and group B recognize the C-terminal tail (residues 202-230) of NS1 protein. MAb-based NS1 Ag sandwich ELISA was designed as sixteen MAb individually conjugated with HRP as the tracer Ab paired with each non-conjugated MAb acted as docking Ab respectively to compare the binding ability of those combinations with E. coli expressed rNS1. Current results indicated that MP-5 (4R11 – 4M2/HRP), MP-9 (4R11 – 4J12/HRP) and MP-10 (4M2 – 4M2/HRP) can detect NS1 Ag from 15 subtypes of IV (H1~H15). It is believed the three MP (MP-5, MP-9 &; MP-10) we studied have potential to be applied on detecting the IV NS1 Ag.
陳廷軒. "Immunogenicity of recombinant protein, adenovirus vector and virus-like particles of the avian-origin influenza H7N9 virus hemagglutinin." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/7ed3h7.
Повний текст джерелаBrahmakshatriya, Vinayak. "Role and Importance of NS1 Protein of Avian Influenza Virus to Grow in the Presence of Interferon and Evaluation of the NS1 Mutant Viruses as Potential DIVA Vaccines." 2009. http://hdl.handle.net/1969.1/ETD-TAMU-2009-08-6992.
Повний текст джерелаTsai, Chi-Yun, and 蔡奇芸. "Differentiating Infected from Vaccinated Poultry on the Basis of Antibodies to NS1, the Nonstructural Protein of Avian Influenza Virus." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/52259799385134576089.
Повний текст джерела中興大學
獸醫學系暨研究所
95
Abstract Avian influenza (AI) is a serious infectious disease caused by avian influenza virus (AIV) belonging to type A Orthomyxovirus. Vaccination programs for controlling avian influenza (AI) in poultry have limitations because of differentiating between vaccinated and virus-infected birds. Nonstructural (NS) proteins can be used as markers because viral infection can induce antibodies against both structural and nonstructural antigens. While immunized with inactivated viral vaccines, animals elicit antibodies from only structural proteins. Two nonstructural proteins (NS1 and NS2) are present in cells when infected with influenza virus. The NS1 protein of AIV is the major nonstructural protein and is highly conserved among AIV subtypes. The antigenic analyses of the purified NSl protein from several subtypes have indicated cross-reactivity among all influenza A virus strains. The presence of an anti-NSl response provides a useful measure in influenza viral infection, and highly circulating anti-HA antibodies. In order to use the immune response of the NSl protein in a routine diagnostic setting, it is necessary to develop an enzyme-linked immunosorbent assay (ELISA) with recombinant NS1. We have used NS1, the conserved nonstructural protein of influenza A virus, as a differential diagnostic marker for influenza virus infection. Experimentally infected poultry were evaluated for the ability to induce antibodies reactive to NS1 recombinant protein produced in Escherichia coli, which was cloned from H6N1 AIV A / Chicken / Taiwan / NCHU-0507 / 99 strain. Immune sera were obtained from SPF chickens inoculated with live AI virus, inactivated semi-purified AI virus, or inactivated AI virus. Animals that received live AI virus, inactivated purified AI virus, or inactivated AI virus were found to possess antibodies against AI virus, as measured by the standard agar gel precipitin (AGP) test. Seroconversion to positive for antibodies to the NS1 protein was achieved in birds experimentally infected with live AI virus, as determined by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. In contrast, animals inoculated with inactivated AI virus had faint seroconversion to positive for antibodies to the NS1 protein, and animals vaccinated with inactivated semi-purified AI virus had low, but detectable, levels of NS1 antibodies. These results demonstrate the potential benefit of a simple, specific ELISA for anti-NS1 antibodies that may have diagnostic value for the poultry industry, this ELISA is useful for serological diagnosis to distinguish poultry infected with influenza viruses from those immunized with inactivated vaccine.
Gung, Ming-Huei, and 鍾明卉. "Expression of the NA protein of avian influenza virus by baculovirus system and its application to rapid subtyping of NA-antibodies." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/47791209665986714093.
Повний текст джерела國立中興大學
獸醫微生物學研究所
94
Avian influenza viruses are members of the orthomyxoviridae family and are grouped into type A influenza virus according to antigenic characteristics of their core proteins. These viruses can be further classified by their surface proteins hemagglutinin (H1-H16) and neuraminidase (N1-N9). During 1999 to 2000, outbroke of low or high pathogenic H7N1 avian influenza occured in Italy and led to a sobering economic loss. In consequence, the government of Italy developed an effective DIVA methodology as a tool for the eradication of AI. The goal of this study is to establish a DIVA methodology for rapid subtyping of NA-antibodies. The method is to use Bac-to-Bac® baculovirus express system to construct recombinant baculovirus containing N1 and N2 genes. Sf9 and High FiveTM insect cells were then used to produce recombinant NA (rNA) protein by infection with recombinant baculovirus. Possible glycosylations of the full length N1 and N2 recombinant proteins were observed based on the molecular weight of SDS-PAGE. However, the amount of recombinant N1 and N2 proteins expressed in insect cells were low. These two rNA proteins were probed with antibodies of 9 NA subtypes by multi-screen channel Western blot analysis, and the result showed that both N1 and N2 recombinant protein could discriminate the N1 and N2 sera, respectively. Moreover, the two rNA proteins were used as antigens to detect antibodies of 9 NA subtypes by indirect immunofluorescence assay (iIFA) analysis, and the result showed that recombinant N1 can discriminate each subtype sera except for the N2. The recombinant N1 and N2 proteins produced in this study could be used to establish a DIVA method that could help the surveillance and prevention of AIV in Taiwan.
黃士至. "Expression of the NA protein of avian influenza virus by baculovirus system and its application to rapid subtyping of NA-antibodies." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/42873017733377022295.
Повний текст джерела國立中興大學
獸醫學系
93
Avian influenza virus is a type A influenza virus. According to virus surface glycoprotein, fifteen HA subtypes(H1-H15)and nine NA subtypes(N1-N9)have been identified. During 1999 and 2000, chickens in Italy were infected by H7N1 viruses of low or high pathogenicity, which resulted in a drastic economic loss. Because of this impact, the government of Italy developed an effective DIVA methodology as a tool for the eradication of AI in Italy. In this study, recombinant baculovirus containing the N1, N3 and N7 genes were constructed by Bac-N-BlueTM or Bac-to-Bac® expression system. High Five insect cells were used to produce recombinant NA(rNA) protein. Possible glycosylations of the full length N3 and N7 recombinant proteins were observed by SDS-PAGE. These two rNA proteins were used to detect antibodies of 9 NA subtypes by multi-screen channel Western blot analysis, and the result showed that N3 recombinant protein could discriminate the N3-specific sera from sera of other subtypes. Moreover, N7 recombinant protein could discriminate the N7-specific sera from sera of other subtypes, except for the N6- and N8-specific sera. The two rNA proteins were used as antigens to detect antibodies of 9 NA subtypes by indirect immunofluorescence assay(iIFA)analysis, and the result showed that sera of the same subtype had strong positive fluorescence against the rNA proteins. Therefore, this iIFA can discriminate sera of N3 and N7 subtypes from sera of other subtypes, especially from N1, N2, N5 and N9 subtypes. The recombinant N3 and N7 proteins produced in this study could be used to establish a DIVA method that could help the surveillance and prevention of AIV.
Oshansky-Weilnau, Christine M. "Suppressor of cytokine signaling protein regulation of respiratory syncytial virus infection and evaluating avian influenza infection of human bronchial epithelial cells." 2009. http://purl.galileo.usg.edu/uga%5Fetd/oshansky-weilnau%5Fchristine%5Fm%5F200908%5Fphd.
Повний текст джерела"Differences in interactions between the nuclear export receptor CRM-1 and the nuclear export protein (NEP) from seasonal and avian influenza a virus lineages: Implications for virus biogenesis, pathogenesis, and transmission." Tulane University, 2011.
Знайти повний текст джерелаacase@tulane.edu