Дисертації з теми "AtPIP2"
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Zhang, Renshan, and 张仁善. "Functional analysis of two arabidopsis purple acid phosphatases : AtPAP2 and AtPAP9." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/211114.
Повний текст джерелаLaw, Yee-song, and 劉益松. "Biological roles of atpap2 in the mitochondria." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2015. http://hdl.handle.net/10722/211155.
Повний текст джерелаZhang, Youjun, and 张有君. "Growth promoting effects of AtPAP2 in potato and camelina." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2011. http://hub.hku.hk/bib/B46476945.
Повний текст джерелаSilverstein, Robert S. "Regulation of the Atp2b2 gene /." Thesis, Connect to this title online; UW restricted, 2006. http://hdl.handle.net/1773/10656.
Повний текст джерелаDouville, Élise. "Structure, assemblage et comportement hydrodynamique d'AtPP2-A1 et AtPP2-A2 chez Arabidopsis thaliana." Nantes, 2010. http://www.theses.fr/2010NANT2093.
Повний текст джерелаDicotyledonous sieve elements typically contain saccharose transported at long distances by mass flow and protein inclusions, called P-Proteins. In Arabidopsis thaliana, P-proteins are found as filaments and immunolocalization studies had confirmed that PP2s are indeed associated to Pproteins. Two PP2 genes were shown to be expressed specifically in the phloem: AtPP2-A1 and AtPP2-A2. Based on observations in microscopy, it was hypothesized that P proteins could, in response to injury of the phloem, participate in the occlusion of sieve tubes by peculiar assembly mechanisms. In order to check this hypothesis, PP2-A1 and PP2-A2 recombinant proteins were produced in E. Coli and their structure and self-assembly properties were studied under different physical chemical conditions, close to phloem sap conditions. Proteins PP2-A1 and PP2-A2 were present in solution as elongated shape oligomers whatever the pH, with a degree of oligomerization N being higher for PP2-A2 than for PP2-A1. These results obtained by small angle X-ray scattering together with observations using transmission electron microscopy suggest that the oligomers self-associate to form an arrangement of filamentous and twisted structures. Increasing ionic strength enhances the proteins self-association. By the contrary, the presence of calcium, sucrose or shear rate do not affect PP2-A1 self-association. Alltogether, these results suggest that the presence of PP2 proteins within phloem sieve tubes do not compromise mass flow
Tietz, Olaf. "Funktionelle Charakterisierung des AtPIN1-Proteins aus Arabidopsis thaliana." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=969066104.
Повний текст джерелаHütter, Michael. "Rolle von CACNA1A, ATP1A2 und SCN1A für die sporadische hemiplegische Migräne." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-101418.
Повний текст джерелаNuntasoontorn, Komsun. "Functional analysis of the Arabidopsis thaliana meiotic proteins AtPCH2 and AtCHR24." Thesis, University of Birmingham, 2014. http://etheses.bham.ac.uk//id/eprint/4921/.
Повний текст джерелаStavridou, Ioanna Stavros. "Novel binding partners of an Arabidopsis phosphatidylinositol phosphate 5-kinase, AtPIPKβ". Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.615136.
Повний текст джерелаAttílio, Lísia Borges. "Transformação genética de laranja doce (Citrus sinensis L. Osbeck) com o gene D4E1 dirigido pelos promotores CaMV35S ou AtPP2." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-23042013-162934/.
Повний текст джерелаBrazil is the largest sweet orange producer in the world. The history of the Brazilian citrus industry is marked by a series of diseases caused by different etiologic agents. Among the diseases affecting the culture, those caused by bacteria are the ones that have caused more significant losses, especially the citrus canker caused by Xanthomonas citri subsp. citri, and huanglongbing (HLB) associated with three \"Candidatus Liberibacter\" bacteria species. Due to the absence of genetic resistance to these diseases in commercial sweet orange cultivars, the genetic transformation is a promising alternative to produce resistant plants. One of the strategies to produce transgenic resistant plants to bacteria is the use of genes that code for antimicrobial peptides, such as D4E1, a antimicrobial synthetic peptide, which has shown efficient results controlling diseases caused by bacteria and fungi in several crops, through in vitro and in vivo experiments. The aim of this study was to produce \'Hamlin\', \'Pêra\' and \'Valencia\' sweet orange transgenic plants, via Agrobacterium tumefaciens, expressing the D4E1 gene driven by the constitutive promoter Cauliflower mosaic virus (CaMV35S) or Arabidopsis thaliana phloem protein 2 (AtPP2), a promoter preferentially expressed in the phloem. It was possible to regenerate 13 \'Hamlin\' transgenic lines, 10 \'Pêra\' transgenic lines and 8 \'Valencia\' transgenic lines bearing the gene construct CaMV35S/D4E1, whereas 19 \'Hamlin\' transgenic lines, 6 \'Pêra\' transgenic lines and 15 \'Valencia\' transgenic lines bearing the AtPP2/D4E1 gene construct were regenerated. The transgenic plants had one to three T-DNA insertion events in the genome. The transgene expression levels in transgenic plants for D4E1 gene driven by the phloem preferential promoter were lower than the transgenic expression levels of the transgene driven by the constitutive promoter. Transgene expression levels results may allow the selection of those plants with higher expression levels of each genetic construct for future multiplication and evaluation for citrus canker and HLB resistance.
Pierrugues, Olivier (19. "Caractérisation moléculaire de deux gènes AtPap2, codant des lipides phosphate phosphatases chez Arabidopsis : implication potentielle d'AtPap2a dans la signalisation du stress." Aix-Marseille 1, 2000. http://www.theses.fr/2000AIX11021.
Повний текст джерелаLa, Camera Sylvain. "Caractérisation fonctionnelle de lipide acyl-hydrolases (LAH) : Etude de l'implication de AtPLP2 dans la résistance aux agents pathogènes chez Arabidopsis thaliana." Université Louis Pasteur (Strasbourg) (1971-2008), 2005. http://www.theses.fr/2005STR13058.
Повний текст джерелаMembrane lipid catabolism is regulated in response to several stresses. Enzymes responsible for lipid hydrolysis are named lipid acyl hydrolases (LAH). An important role anticipated for such enzymes is to be involved in antimicrobial resistance and to provide precursors for the biosynthesis of oxylipins that are regulatory fatty acid derivatives. Exploration of the Arabidopsis thaliana genome has revealed the existence of numerous structural families of potential LAH genes, with members being upregulated in response to biotic stress. We have given priority to the functional study of Arabidopsis LAH related to patatin. This family comprises 9 members, two of which (AtPLP2 and AtPLP7) being strongly upregulated in leaves challenged with pathogens. AtPLP2 protein accumulation in response to the fungus Botrytis cinerea or Pseudomonas syringae bacteria is dependent on jasmonic acid and ethylene signaling. Expression of a AtPLP2-GFP fusion and biochemical analysis of recombinant AtPLP2 indicates that AtPLP2 encodes a cytoplasmic LAH. Transgenic plants with altered levels of AtPLP2 protein were generated and assayed for pathogen resistance. Unexpectedly, AtPLP2 expression increases B. Cinerea colonization and susceptibility to avirulent bacteria whereas silenced plants displayed enhanced resistance. Collectively, the data indicate that AtPLP2-encoded lipolytic activity is recruited by pathogens with different lifestyles to facilitate host colonization. Particularly, AtPLP2 potentiates plant cell death upon infection by B. Cinerea and reduces the efficiency of the hypersensitive response known to normally restrict avirulent bacteria multiplication. This global Arabidopsis LAH study opened some perspectives in identifying several candidates genes for detailed functional studies. Tools like numerous LAH knock-out mutants obtained will be the basis of our future work to decipher fatty acid mobilisation processes during plant defense responses
Rousselle, Anthony. "Role of the (Pro)renin Receptor [(P)RR/ATP6ap2] in Osteoclast and Macrophage Physiology." Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18599.
Повний текст джерелаA decade ago, the (pro)renin receptor [(P)RR] was discovered and depicted as a new component of the renin-angiotensin system. However, recent studies have put in evidence that the (P)RR associate with and regulate the vacuolar H+-ATPase (V-ATPase), hence its other name vacuolar H+-ATPase associated protein 2 (ATP6ap2). In osteoclasts, V-ATPases are mainly located at the plasma membrane facing the bone surface and extrude protons into the extracellular space. Mice with genetic deletion of various V-ATPase subunits are characterized by an increase of bone mass (osteopetrosis). In this work, we found that the (P)RR is highly expressed in mature osteoclasts in vitro and in vivo. Mice with genetic deletion of the (P)RR in osteoclasts developed a complex bone phenotype characterized by a reduced bone density. Osteoclasts lacking (P)RR displayed increased differentiation and/or activity in vitro and in vivo. We therefore suggest that the (P)RR does not directly regulate V-ATPases located at the plasma membrane but rather interferes with osteoclast physiology through other mechanisms. Macrophages are professionalized phagocytes crucial for immune response. Phagocytosis is an essential cellular process, which requires lysosomal V-ATPases for degradation of engulfed pathogens. We generated transgenic rats with a conditional depletion of the (P)RR with the use of a doxycycline-induced shRNA expression system. Efficient (P)RR depletion in macrophages was accomplished by doxycycline treatment in vivo in drinking water and in vitro in culture medium. In this work, we found that the impairment of vesicular pH occurs lately after (P)RR deletion. Also, we found that (P)RR deletion did not impair neither phagocytosis nor endocytosis but rather perturbed the recycling of the transferrin receptor to the plasma membrane.
Ceciliato, Paulo Henrique de Oliveira. "Dissociabilidade das funções de inibição da expansão celular e de alcalinização do peptídeo AtRALF1 e identificação dos aminoácidos determinantes da atividade de alcalinização." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-23062015-155306/.
Повний текст джерелаRALFs are 5kD peptide hormones that negatively regulates cell expansion. Among the biological activities of the RALF peptide, the extracellular alkalinization and cellular expansion inhibition were previously suggested to be associated, once the extracellular acidification is required to cell expansion. Usually, the alkalinization and cell expansion inhibition activities are evaluated in two different assays, the cell suspension medium alkalinization and the plantlet root growth inhibition. We manage to set an assay in which both cell expansion inhibition and extracellular alkalinization activities could be evaluated. Using the package suspension cell volume through decantation as a value of cell expansion, we evaluated the relation between alkalinization and cell expansion inhibition in different conditions. When the MES buffer or the pre-treatment with Fusicoccin was used to arrest the AtRALF1 extracellular alkalinization, the package cell volume inhibition activity was not affected. There are 9 RALF peptides encoded in arabidopsis plants that are closely related with the first isoform isolated from tobacco. With exception of the AtRALF4, all those isoforms are able to alkalinize the extracellular medium and arrest root growth. Comparing the AtRALF4 with other eight isoforms, we verified that are few different amino acids between them, suggesting that those amino acids could be essential for the biological activities. The rescue of one of those amino acids, AtRALF4(N92A), was able to regain the root growth inhibition activity of the AtRALF4 peptide, although the extracellular alkalinization activity was not restored. When three other AtRALF4 amino acids were substituted by their AtRALF1 correspondent, there is a partial rescue of the extracellular alkalinization activity, but no alterations in the root growth inhibition. We had recently shown that the AtRALF1 peptide induces the expression of genes related to cell wall rearrangement. The quantitative PCR analyses demonstrates that only the mutant peptides that are able to arrest root growth are also able to induce the gene expression. When submitted to a pH indicator, it was verified that AtRALF1 overexpressing plants acidifies it during its growth, as much as wild type plants.The defense peptide AtPEP1, similarly of AtRALF1, also triggers extracellular alkalinization. The use of proton-pump chemical modulators to simulate or arrests AtPEP1 extracellular alkalinization activity suggests that the gene expression of the AtPEP1-responsive genes is not related to changes in plasma membrane proton pump activity. Finally, when double mutants for both the AtPEP1 receptors (atpepr1/r2) were submitted to a pH gel indicator, it was seen that they alkalinize the rhizosphere pH in the presence of the AtPEP1 peptide. Our data suggests that the extracellular alkalinization and arrest of cell expansion activities are dissociated. The proton pump modulation activity may not be only a secondary messenger, but another source of information independent and yet to be explored.
Friml, Jirí. "Isolation and characterization of novel AtPIN genes from Arabidopsis thaliana L." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961738510.
Повний текст джерелаGosch, Jonas [Verfasser]. "Epigenetische Suppression von RASAL1 und ATP2A2 als Biomarker und Therapieansatz bei kardialer Fibrogenese / Jonas Gosch." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://d-nb.info/1214440169/34.
Повний текст джерелаOttenschläger, Iris. "Gravity regulated differential auxin transport in Arabidopsis roots and the search for interaction partners of AtPIN1." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964941848.
Повний текст джерелаLefebvre, Valerie. "Identification of Novel Parkinson’s Disease Genes Involved in Parkin Mediated Mitophagy." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/30222.
Повний текст джерелаAriyaratne, Menaka M. "A new perspective on polyamine biosynthesis and transport in arabidopsis thaliana." Bowling Green State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1555693507751475.
Повний текст джерелаColeman, Jonathan Allan. "P4-ATPase structure-function relationships : mechanism and roles of ATP8A2-CDC50A in aminophospholipid transport, protein trafficking, and visual disorders." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/44073.
Повний текст джерелаBurckle, Céline. "Le Récepteur de la prorénine/rénine-RR/ATP6AP2 : données de biologie cellulaire : poids moléculaire, topologie, localisation subcellulaire : approche fonctionnelle." Paris 7, 2006. http://www.theses.fr/2006PA077227.
Повний текст джерелаThe function of RR/ATP6AP2, described as a new receptor for prorenin/renin, is elusive in vivo. Controversies concerning its subcellular localization are confusing considering the proposed function of vascular cell surface receptor. Here, we readdressed the question of the sub cellular localization of RR/ATP6AP2, focusing on the detection of the endogenous protein. Then we used animal models of gene invalidation and gene overexpression to approach the in vivo function of RR/ATP6AP2. Our results underline that RR/ATP6AP2 might have a dual function possibly reflecting an evolutionary divergence. The RR/ATP6AP2 C-terminal part of the protein, corresponding to the m8-9 fragment, is highly conserved in vertebrates and invertebrates. This "ancestral" domain might account for the early developmental defect observed in zebrafish and suspected in mouse. This function is clearly not related to the renin angiotensin System, and might be linked to the vacuolar H+-ATPase. The N-terminal part of the protein might have acquire a prorenin/renin binding capacity in vertebrates
Bastian, René. "Characterisation of AtPNP-A - a novel arabidopsis thaliana gene with role in water and salt homeostasis." Thesis, University of the Western Cape, 2009. http://hdl.handle.net/11394/2818.
Повний текст джерелаPlant natriuretic peptides (PNPs) are a novel class of extracellular, systemically mobile molecules that elicit a number of plant responses important in homeostasis and growth. Natriuretic peptides were first identified in vertebrates where they play a role in the regulation of salt and water balance. Subsequent experimental investigations have identified the presence of a natriuretic peptide hormone system in plants. While PNPs have been implicated in various physiological responses such as stomatal guard cell movements and regulation of net water uptake, its biological role has remained elusive. Here we have used co-expression and promoter content analysis tools to understand the biological role of the Arabidopsis thaliana PNP (AtPNP-A). The analysis of AtPNP-A and its co-expressed genes revealed that genes annotated as part of the systemic acquired resistance (SAR) pathway were over-represented, thus suggesting that AtPNP-A may function as a component of plant defense responses and specifically, SAR. The results further show that AtPNP-A shares many characteristics with pathogenesis related (PR) proteins in that its transcription is strongly induced in response to pathogen challenges, thus implying a newly described role for AtPNP-A in pathogen attack. Additional tissue expression analysis also indicated distinct localization of PNP activity in sepals and transcriptional meta-analysis showed that AtPNP-A may play a role in starch breakdown. Therefore, together with the finding that AtPNP-A plays a role in regulating phloem transport, we also hypothesize that AtPNP-A may play a role in phloem unloading in sepals to assist processes such as seed formation in plants. In plants, the second messenger, guanosine 3’,5’-cyclic monophosphate (cGMP) mediates a whole range of important processes including salinity tolerance, disease resistance, drought tolerance and responses to light. Since PNPs regulate water and salt homeostasis via a cGMP-dependent signaling pathways, it is thus important to analyse the transcriptome induced by the second messenger (cGMP) in Arabidopsis thaliana to give a better understanding of its mechanism of action. This study was also supplemented by the analysis of the gibberellic acid (GA) dependent transcriptome, since cGMP also plays a role its transcription pathway. This data analysis, together with promoter content investigation, revealed that genes upregulated after cGMP treatment and down-regulated in the GA insensitive mutant (ga1-3) were enriched with a GA response element (GARE), while no GARE enrichment were observed in genes up-regulated in the ga1-3 mutant. These findings suggest that GARE is indicative of GA-induced and cGMP-dependent transcriptional up-regulation. Gene ontology analysis confirmed previous reports that cGMP is involved in ion homeostasis and indicated that the transcriptional cGMP response is bi-polar in the sense that both genes up- and down-regulated in response to cGMP is involved in cation transport. Additionally, ab initio analysis of genes transcriptionally dependent on cGMP identified CHX8 as a hub gene and promoter content of CHX8 co-expressed genes show enrichment of the GARE motif. The fact that CHX8 has its highest expression levels during male gametogenesis and pollen tube growth, together with our findings, suggest that GA-induced and cGMP- dependent genes may play a key role in ion and water homeostasis in the male gametophyte. Finally, we propose that the type of analysis undertaken here can yield new insights into gene regulation networks and inform experimental strategies to unravel complex transcription regulatory systems under different developmental and stimulus specific conditions.
South Africa
Goussard, Sébastien. "Etude de la localisation mitochondriale de l'ARNm ATP2 chez Saccharomyces cerevisiae." Paris 7, 2012. http://www.theses.fr/2012PA077113.
Повний текст джерелаMy thesis is upon mRNA's localized translation at the proximity of mitochondria. The translation localization phenomena allows an mRNA to be translated at the same site it's protein acts. 60% of nuclear genome encoded mitochondrial proteins are translated on the mitochondrial surface. There is two classes of mRNA localized to mitochondria. Class l's mRNA necessit the RNA binding protein Puf3p to be localized, which is not the case of class IF s mRNA. The identification of cis acting signals in ATP2 mRNA, a class II transcript, has been done using a method combining FISH experiments and statistical analyzes of pictures, allowing the determination of the proportion of cells where the mRNA is localized on mitochondria's surface. The translation, the MTS (mitochondrial targetting sequence) and two regions, RI and R2, are essentials to ATP2's mRNA mitochondrial localization. During my Ph. D, I reduced region R2 size from half ATP2 length to the 200 last bases. I showed that two RNA formed of the same coding regions but in a different order are not enriched in the same proportion in mitochondrial fraction. The proportion of cells where the mRNA is localized to mitochondria double wether the raporter gene LacZ is at the 3' or 5' of ATP2 gene. I've done sucrose gradient polysomes separation experiments to establish the translations dynamic of those mRNA. It seems that the translation of mRNA highly enriched in mitochondria's fraction is less efficient
Cannata, Serio Magda. "Mutations in V-ATPase assembly factors cause Congenital Disorder of Glycosylation (CDG) with autophagic liver disease Mutations in the X-linked ATP6AP2 cause a glycosylation disorder with autophagic defects Mutations in the V-ATPase assembly factor VMA21 cause a congenital disorder of glycosylation with autophagic liver disease." Thesis, Sorbonne Paris Cité, 2019. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=2277&f=17696.
Повний текст джерелаThe V-ATPase is a large complex involved in the acidification of intracellular organelles. It is formed by a proton pore (V0 sector) and ATP hydrolysis domain (V1 sector). Pioneering studies in S. cerevisiae have shown that the assembly of the V0 sector occurs in the endoplasmic reticulum (ER) under the guidance of 5 assembly factors: Vma21p, Vma12p, Vma22p, Pkr1p and Voa1p. The newly assembled V0 sector is then escorted by Vma21p to the cis-Golgi, where it will bind the V1 sector to constitute a functional holoenzyme. How the assembly works in mammalian systems is currently still unclear. Yet, it was recently shown that all the assembly factors except Pkr1p are conserved in mammals and that mutations in three of them, ATP6AP1, TMEM199 and CCDC115, were identified to cause a new subgroup of congenital disorders of glycosylation (CDG). Using human genetics and functional validation, I identify in the first part of my thesis a novel mammalian assembly factor called ATP6AP2 that causes a similar CDG-like disease when mutated. Apart from glycosylation defects, patients with missense mutations in ATP6AP2 show steatotic liver disease, cognitive defects and immune defects. Previously exon-skipping mutations in ATP6AP2 had been associated with a late onset cerebral disease, with Parkinsonism and epilepsy. Our work revealed that ATP6AP2 missense mutations lead to defective V-ATPase activity, subsequently causing reduced organellar acidification, lysosomal degradation and autophagic flux. Because of this, clearance of lipid droplets cannot take place in the autolysosomes, giving rise to the steatotic phenotype in the patients hepatocytes. Consistent with the similar clinical phenotype, we found that ATP6AP2 interacts with V0 assembly factors, while the missense mutations reduced the interaction, suggesting a compromised V-ATPase assembly in the patients. By contrast, in patients with exon-skipping mutation we found normal glycosylation of serum proteins and no effect on the interaction between ATP6AP2 and ATP6AP1, suggesting that the missense mutations have a stronger impact on overall ATP6AP2 function than the exon-skipping ones. These results shed light on the V-ATPase assembly in the ER and suggest that ATP6AP2 is an additional mammalian member of the assembly factors. In the second part of my thesis, I demonstrate that mutations in VMA21 also cause CDG with liver disease. Previously, mutations of VMA21 had been associated with an X-linked myopathy with excessive autophagy (XMEA), characterized by progressive vacuolation and atrophy of skeletal muscle. Yet, we were able to identify VMA21 mutations with a similar clinical phenotype compared to the other V-ATPase assembly factor deficiencies. Using patient fibroblasts, we tested the functional impact of the newly identified VMA21 mutations on V-ATPase assembly and function, with the attempt to highlight the differences between with the XMEA ones. First, we could show that VMA21 mutations are hypomorphic and reduce both Vma21 mRNA and protein expression. Second, VMA21 mutations cause autophagic defects with decreased lipid droplet degradation, similar to those observed in ATP6AP2-deficient cells. Finally, VMA21 fibroblasts showed an accumulation of unesterified cholesterol in vesicular structures, similar to what has been reported for the lysosomal storage disease Niemann-Pick type C (NPC). The sequestration of cholesterol in lysosomes triggers lipogenic pathways mediated by the sterol response element-binding protein (SREBP), most likely leading to hypercholesterolemia in the patients. Altogether, our results show that V-ATPase deficiencies are a novel group of metabolic syndromes that affect lysosomal/autophagic homeostasis. Studying these rare V-ATPase assembly disorders that are featured by liver steatosis as a unifying pathology may lead to a better understanding of the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is a common problem in metabolic syndrome
Trivilin, Ana Paula. "Superexpressão do gene codificante do peptídeo AtPep1 em A.Thaliana visando a obtenção de resistência à isolados de diferentes espécies do gênero Pythium." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/15865.
Повний текст джерелаPlants are exposed to a range of pathogens that use several strategies to infect their host. In response, the plants express different mechanisms of defense, in which the recognition of conserved pathogen molecules by plant specific receptors triggers the defence responses. The discovery of exogenous and endogenous signals that regulate the expression of defense genes in Arabidopsis thaliana has helped the understanding of the mechanisms of defense. The development of a strategy of overexpression for evaluating the role of endogenous peptide AtPep1 in resistance to isolates of different species of the genus Pythium is important for understanding the mechanism of defense and its future use in resistant cultivars to the pathogen. Therefore, experiments that were performed consisted in the inoculation of A. thaliana with isolates of the of P. graminicola, P.inflatum, P. deliense, and P. ultimum species. The plants were evaluated for the presence of disease symptoms. In addition to that, the gene coding the peptide AtPep1, PROPEP1 was isolated from A. thaliana, ligated in the binary vectors pGSA1427 or pEGAD, and transformed in Agrobacterium tumefaciens. Cells of A. tumefaciens containing the plasmids with or without PROPEP1 were used to floral transformation of A. thaliana. The transformed plants were selected for the presence of the gene that confers tolerance to the herbicide ammonium glufosinate and, in the case of plants transformed with the vector pEGAD, they were also selected for the presence of GFP (green fluorescent protein). The presence of the inserted gene was confirmed by PCR followed by sequencing. The transformed plants were evaluated on the accumulation of mRNA from PROPEP1 and resistance to isolates of the genus Pythium. The results indicate that A. thaliana wild type plants are susceptible to isolates from various species of the genus Pythium. The pEGAD-AtPep plants showed an increase in the expression of PROPEP1 of up to 4.000 times more than that was found in control plants. Such high accumulation of PROPEP1 in pEGAD-AtPep and pGSA-AtPep plants was shown to be important in resistance to the isolate of P. deliense. The evidences support the role of the peptide AtPep1 as an endogenous elicitor that is generated in response to pathogens and their PAMPs.
Rousselle, Anthony [Verfasser], Michael [Gutachter] Bader, Achim [Gutachter] Leutz, and Uwe [Gutachter] Kornak. "Role of the (Pro)renin Receptor [(P)RR/ATP6ap2] in Osteoclast and Macrophage Physiology / Anthony Rousselle ; Gutachter: Michael Bader, Achim Leutz, Uwe Kornak." Berlin : Humboldt-Universität zu Berlin, 2017. http://d-nb.info/1189328143/34.
Повний текст джерелаThomazella, Maria Cristina Dias. "Efeito da dieta tipo Mediterrânea na função endotelial e inflamação da aterosclerose: estudo comparativo com a dieta TLC (\"Therapeutic Lifestyle Changes\", no NCEP-ATPIII)." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/5/5131/tde-24062010-143245/.
Повний текст джерелаThe Mediterranean Diet (MD) has been widely studied with respect to epidemiology, but mechanisms whereby the Mediterranean Diet (MD) is cardioprotective are unclear. This is partly because of the difficulties of adherence in clinical trials of dietary intervention, particularly trials comparing it to traditional lipid-restraining diets, e.g., Therapeutic Lifestyle Changes Diet (TLCD) from National Cholesterol Education Program ATPIII. We performed a controlled, non-randomized clinical trial comparing the cardiovascular risk profile of the Mediterranean Diet (MD) versus the TLC Diet (TLCD) in 40 selected, highly-homogeneous, and intensively medicated patients with coronary heart disease (45-65 years, males, at least one coronary event over prior 2 years). In addition, we sought to investigate both diets effects on inflammation, endothelial dysfunction and oxidative stress, all key factors in atherogenesis and particularly important in secondary prevention. Dietary/cultural habits were the basis to allocate patients for 3 months to either MD (n = 21; rich in whole grains, vegetables, fruits, nuts 10g/day, extra-virgin olive oil 30g/day, red wine 250ml/day) or TLCD (n = 19; plus phytosterols 2g/day). Specific scores showed that both diets had >90% adherence. Some effects were common to both diets. Patients in both groups showed a significant reduction in weight, body mass index, body composition and blood pressure. Also, both groups presented a reduction in plasma levels of ADMA and L-arginine/ADMA ratio. Endothelial-dependent brachial artery reactivity remained unaltered in both groups. However, patients under MD and TLCD improved flow velocity at baseline (prior to hyperemia). Nevertheless, other effects were specific to each diet. With MD, there was significant decrease in leukocyte count vs. TLCD (p = 0.03) and average increase in HDL-cholesterol by 3 mg/dL (p = 0.053) versus TLCD. The brachial arterials basal diameter increased with MD but not with TLCD. However, with TLCD there was a statistically significant reduction of lipid variables: total cholesterol, LDL-cholesterol (p < 0.05) and oxidized LDL (p = 0.009) vs. MD even though the ratio of oxidized / total LDL remained unaltered. Plasma and serum levels of apolipoprotein A-1, lipoprotein(a), glucose, myeloperoxidase, sICAM, sVCAM, and glutathione reduced/oxidized ratio in plasma and erithrocytes also remained unaltered in both groups. Together, these results demonstrate a pattern of effects of MD and TLCD compatible with cardiovascular risk reduction, in secondary prevention, even in intensely medicated patients. Although these effects were equivalent between MD and TLCD, they seem to be mediated by some common mechanisms, as well as by each diets specific mechanisms
Suifan, Taghrid. "Team effectiveness : a test of in-put process-output." Thesis, Aston University, 2010. http://publications.aston.ac.uk/19120/.
Повний текст джерелаSchäfer, Simon Thomas [Verfasser]. "ATP6AP2 is critically involved in adult hippocampal neurogenesis and reveals stage-specific functions for Wnt/ß-Catenin and Wnt/Planar Cell Polarity (PCP) signaling / Simon Thomas Schäfer." Greifswald : Universitätsbibliothek Greifswald, 2015. http://d-nb.info/1069389331/34.
Повний текст джерелаHeinz, Anja [Verfasser]. "Die Bedeutung von AtpI für die F1Fo-ATPase in Escherichia coli und Cupriavidus metallidurans / Anja Heinz." Halle, 2017. http://d-nb.info/1142920488/34.
Повний текст джерелаDau, Andressa Minussi Pereira. "Sistema renina-angiotensina nas células da teca e granulosa durante a ovulação e luteinização em bovinos." Universidade Federal de Santa Maria, 2017. http://repositorio.ufsm.br/handle/1/11578.
Повний текст джерелаThe objective of present study was to investigate (Pro)renin receptor function in the theca and granulosa cells during the preovulatory period and luteinization in cattle. During the initial preovulatory period, prorenin induced the resumption of oocyte meiosis even in the presence of follicular hemisections or forskolin. In granulosa cells, pró-renina did not increase LHinduced epiregulin (EREG) mRNA after 6 h of culture. Treatment with prorenin plus LH increased amphiregulin (AREG) and prostaglandin synthase 2 (PTGS2) mRNA in granulosa cells. The absence of prorenin effect to stimulate EREG, AREG, and PTGS2 in granulosa cells was established using different combinations of treatments with prorenin and/or aliskiren ([P]RR inhibitor) and/or LH. Treatment of granulosa cells with LH plus EGFR antagonist (AG1478) did not regulate prorenin and (P)RR after 6 h of culture. This result was confirmed in vivo using a model of intrafollicular treatment with AG1478 and intramuscular treatment with GnRH. Finally, (P)RR protein and transcripts for prorenin and pro-fibrotic genes increased in the granulosa cells from 12 h post-GnRH. In the theca cells, (P)RR mRNA and protein increased 6 h after treatment of cows with GnRH. The LH effect to stimulate (P)RR transcript was confirmed in vitro. Intrafollicular treatment with aliskiren did not reduce the ovulation rate. In cultured theca cells, AREG and EREG mRNA were not significantly expressed and ADAM17 was not stimulated by prorenin. Intrafollicular injection of AG1478 did not regulate LH-induced (P)RR, although increased CYP17A1 protein. Prorenin did not induce androstenedione and testosterone synthesis in cultured theca cells. In the corpus luteum, prorenin and (P)RR mRNA were increased at day 10 of estrous cycle compared to day 5, but were not regulated by prostaglandin in vivo, as observed for profibrotic genes. Intrafollicular treatment with aliskiren reduces serum progesterone levels in cows that ovulated. Prorenin role in progesterone synthesis through (P)RR was also evidenced in vitro. Moreover, prorenin induced ERK1/2 phosphorylation in luteal cells, although ERK1/2 inhibition (PD0325901) did not completely inhibit prorenin-induced progesterone synthesis, as evidenced using AG1478. In summary, these results demonstrate that prorenin and (P)RR are stimulated by LH at the end of the preovulatory period and, therefore, they are not related to genes regulated by LH at the initial ovulatory process in granulosa cells; (P)RR is stimulated by LH in the theca cells independently of EGFR; and prorenin stimulate progesterone synthesis through (P)RR, which involves ERK1/2 and EGFR participation. In conclusion, (P)RR is upregulated in granulosa and theca cells after gonadotropins peak and prorenin/(P)RR play an important role in the resumption of oocyte meiosis and on progesterone synthesis in the corpus luteum in cattle.
O objetivo do presente trabalho foi investigar a função do receptor de (pro)renina [(P)RR] nas células da teca e da granulosa durante o período pré-ovulatório e luteinização em bovinos. No início do período pré-ovulatório, pró-renina reiniciou a meiose oocitária bloqueada tanto pelas metades foliculares, quanto por forscolina. Nas células da granulosa, pró-renina não aumentou a expressão de RNAm para epirregulina (EREG) que foi induzido por LH após 6 horas de cultivo. Pró-renina mais LH aumentaram a expressão de RNAm para anfirregulina (AREG) e prostaglandina endoperoxidase sintetase-2 (PTGS2). Contudo, a ausência do efeito de prórenina para estimular o RNAm para EREG, AREG e PTGS2 nas células da granulosa foi evidenciada utilizando as diferentes combinações de tratamento com pró-renina e/ou alisquireno [inibidor do (P)RR] e/ou LH. O tratamento das células da granulosa com LH e antagonista de EGFR (AG1478) não regularam o RNAm para pró-renina e (P)RR após 6 horas de cultivo. Esse resultado foi confirmado in vivo, utilizando um modelo de tratamento intrafolicular com AG1478 e GnRH intramuscular em vacas. Por fim, (P)RR e o RNAm para pró-renina e genes prófibróticos aumentaram nas células da granulosa a partir das 12 horas após tratamento de vacas com GnRH. Nas células da teca, a expressão de (P)RR aumentaram 6 horas após tratamento de vacas com GnRH. O estimulo de LH sobre o transcrito de (P)RR foi confirmado in vitro. O tratamento intrafolicular com alisquireno não reduziu a taxa de ovulação. No nosso cultivo de células da teca, a expressão de RNAm para AREG e EREG não foi significativa e ADAM17 não foi estimulado por pró-renina. Injeção intrafolicular com AG1478 não regulou (P)RR estimulado por LH, mas aumentou a proteína para CYP17A1. Pró-renina não induziu a síntese de androstenediona e testosterona no nosso sistema de cultivo. No corpo lúteo, RNAm para pró-renina e (P)RR foi aumentado no dia 10 do ciclo estral comparado ao dia 5 e não foram regulados por prostaglandina in vivo, como observado para os genes pró-fibróticos. O tratamento intrafolicular com alisquireno diminuiu os níveis de progesterona plasmática em vacas que ovularam. O papel de pró-renina na síntese de progesterona através de (P)RR também foi evidenciado in vitro. Ainda, pró-renina induziu a phosphorilação de ERK1/2 nas células luteais, embora o bloqueio de ERK1/2 (PD0325901) não inibiu completamente a síntese de progesterona induzida por pró-renina, como evidenciado pelo uso de AG1478. Em resumo, esses resultados demonstram que pró-renina e (P)RR são estimulados por LH no final do período pré-ovulatório e, portanto, não estão relacionados com os genes regulados por LH no início do processo ovulatório nas células da granulosa; (P)RR é estimulado por LH nas células da teca de forma independente de EGFR; e a pró-renina estimula a síntese de progesterona via (P)RR envolvendo a participação de ERK1/2 e EGFR neste processo. Em conclusão, (P)RR é regulado positivamente nas células da granulosa e da teca após o pico de LH e a pró-renina/(P)RR possui um importante papel no reinicio da meiose oocitária e na síntese de progesterona pelo corpo lúteo em bovinos.
Vogel, Lukas Hermann [Verfasser], Jörg [Akademischer Betreuer] Peters, Jörg [Gutachter] Peters, and Sigrid [Gutachter] Hoffmann. "Einfluss der (Pro)Renin Rezeptor ((P)RR, ATP6ap2) Downregulation auf primäre Zilien und den Zellzyklus in As4.1 Zellen / Lukas Hermann Vogel ; Gutachter: Jörg Peters, Sigrid Hoffmann ; Betreuer: Jörg Peters." Greifswald : Universität Greifswald, 2021. http://d-nb.info/1230552936/34.
Повний текст джерелаDrager, Robert Gray. "Structure and transcript processing of a Euglena gracilis chloroplast operon encoding genes rps2, atpI, atpH, atpF, atpA and rps18." Diss., The University of Arizona, 1993. http://hdl.handle.net/10150/186334.
Повний текст джерелаMorch, Anica [Verfasser], Jörg [Akademischer Betreuer] Peters, Michael [Gutachter] Bader, and Jörg [Gutachter] Peters. "Die Lokalisation des (Pro)Reninrezeptors in renalen, kardialen und neuronalen Zellen und Geweben sowie seine Funktion als akzessorisches Protein ATP6ap2 der vakuolären H+-ATPase / Anica Morch ; Gutachter: Michael Bader, Jörg Peters ; Betreuer: Jörg Peters." Greifswald : Universität Greifswald, 2019. http://d-nb.info/1195140894/34.
Повний текст джерелаCarreño, Oriel. "Anàlisi genètica i funcional de la migranya hemiplègica i la migranya comuna." Doctoral thesis, Universitat de Barcelona, 2011. http://hdl.handle.net/10803/85723.
Повний текст джерелаThis Thesis is focused in migraine genetics, migraine is a prevalent neurological disorder characterized by recurrent episodes of headache. This research was divided in two areas according to the genetic basis of the disorders; on the one hand we studied the common migraine with a complex genetics, on the other hand we studied the rare mendelian forms of familial hemiplegic migraine (FHM). To understand more the genetic basis of the common migraine a case-control association study approach was used with candidate genes. For that purpose, around 550 patients with migraine and their corresponding control group were selected. In order to analyze their involvement in the genetic susceptibility to migraine, we chose genes encoding for channels of the heterogeneous superfamily of Transient Receptor Potential (TRP) which are known to be involved in the nociceptive pathway. In the particular case of FHM, a monogenic form of the disorder, there are three genes known to be involved in the FHM (CACNA1A, ATP1A2 and SCN1A), whose encoded proteins are playing a relevant role in the neurotransmission of the glutamate. Functional analysis of the mutations causing FHM have shown ultimately an increased neurotransmission release. CACNA1A previous studies reveled a gain-of-function effect from FHM mutations, unlike mutations on ATP1A2 that present a loss-of-function effect. Our work consisted on identifying mutations in patients by direct sequencing. If the mutations were interesting enough vector constructions were generated for functional studies in eukaryotic cells. This work gave rise to three publications: First; the identification of a change that modulates the function of the CACNA1A channel. This study contributes to explain the genetic contribution in the clinical heterogeneity of one family and to know more about the molecular mechanism of the P/Q calcium channel. Second; a report of a patient that presents an acute stratial necrosis that had clinical relevance because of the early onset of the neurological symptoms previous to the hemiplegic attacks. Third; a mutational screening of ATP1A2 and CACNA1A genes in 19 patients with FHM. 5 previously described mutations and two new mutations were found. Functional studies were carried out for the newly mutations.
Liu, Li. "Roles of PMCA Isoforms in Ca2+-Homeostasis and Contractility of Bladder Smooth Muscle: Evidence from PMCA Gene-Ablated Mice." Cincinnati, Ohio : University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view.cgi?acc_num=ucin1178307168.
Повний текст джерелаAdvisor: Richard J. Paul. Title from electronic thesis title page (viewed Apr. 4, 2009). Keywords: PMCA (human gene symbols; ATP2B); SERCA2 (human gene symbols; ATP2A2); NCX; bladder smooth muscle; Ca²⁺ homeostasis; gene-altered mice. Ca²⁺ waves; Ca²⁺ sparks; Fura-PE3; Fluo-4; Indo-1; multi-photon microscopy. Includes abstract. Includes bibliographical references.
Heckwolf, Marlies. "Funktionelle Charakterisierung der Arabidopsis thaliana Aquaporine AtPIP1;2 und AtPIP2;3." Phd thesis, 2010. https://tuprints.ulb.tu-darmstadt.de/2042/1/Dissertation_neu.pdf.
Повний текст джерелаHeckwolf, Marlies [Verfasser]. "Funktionelle Charakterisierung der Arabidopsis thaliana Aquaporine AtPIP1;2 und AtPIP2;3 / von Marlies Heckwolf." 2010. http://d-nb.info/1000904342/34.
Повний текст джерелаROBINSON, Whitney Drummond. "FEEDING HUNGRY PLANTS: THE SECRETED PURPLE ACID PHOSPHATASE ISOZYMES AtPAP12 AND AtPAP26 PLAY A PIVOTAL ROLE IN EXTRACELLULAR PHOSPHATE SCAVENGING IN ARABIDOPSIS THALIANA." Thesis, 2012. http://hdl.handle.net/1974/7394.
Повний текст джерелаThesis (Master, Biology) -- Queen's University, 2012-08-23 11:36:45.722
Müller, Andreas. "Genetische und molekulare Analyse des AtPIN2-Gens aus Arabidopsis thaliana L. /." 1997. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=008193395&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
Повний текст джерелаSu, Hung Chia, and 蘇紅嘉. "E-box regulation of human Atp1a2 promoter." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/24864241454697444679.
Повний текст джерела長庚大學
生物醫學研究所
103
Circadian rhythm is an approximately 24-hour cycle of our bodily functions including physiology and behavior. In mammals, the circadian rhythm is generated by the central clock located in the hypothalamic suprachiasmatic nuclei (SCN). The SCN neurons exhibit a circadian rhythm in spontaneous firing rate and intracellular Ca2+ concentrations, both being higher during the day than at night. We previously find that the Na+-K+ ATPase (NKA) also has higher daytime activity. The energy-demanding and electrogenic nature of the NKA and its ability to regulate intracellular Na+ and Ca2+ via Na+/Ca2+ exchanger suggest that the NKA may play an important role in the integration of energy metabolism, intracellular ion homeostasis, and neuronal excitability in the SCN. We suspected that the NKA be a clock-controlled gene. Indeed, the rat NKA α2 subunit gene (Atp1a2) has E box (CANNTG) sequence in its promoter region. The aim of this study was to determine the presence of E box in human Atp1a2 promoter and whether the E box may regulate the promoter activity in a clock-controlled manner. We identified E boxes of human Atp1a2 promoter by using promoter deletion assay and determined their activity by using transient transfection assay and luciferase reporter assay in human embryonic kidney 293 (HEK293) cells. Series of Atp1a2 -luciferase clones were constructed by promoter deletion assay. E box mutation clones were further constructed to evaluate which E box is the major regulatory element. And, we constructed stable clones and using luciferase reporter assay to detected whether E boxes of Atp1a2 promoter have circadian rhythm. Transient transfection assay and luciferase reporter assay show that the F2 clone that contains proximal E box has significantly higher luciferase activity than other clones, and F1 clone that contains proximal and distal E boxes has significantly lower luciferase activity than F4 clone which has no E box. E box mutation clones show that as long as distal E box exist, proximal E box can not represent its ability. Stable transfection assay show that stable clone’s luciferase activity trend consistent with transient clone. Our results indicate the distal E box acts as dominant negative regulator and proximal E box acts as positive regulator.
Tietz, Olaf [Verfasser]. "Funktionelle Charakterisierung des AtPIN1-Proteins aus Arabidopsis thaliana / vorgelegt von Olaf Tietz." 2002. http://d-nb.info/969066104/34.
Повний текст джерелаGosch, Jonas. "Epigenetische Suppression von RASAL1 und ATP2A2 als Biomarker und Therapieansatz bei kardialer Fibrogenese." Doctoral thesis, 2020. http://hdl.handle.net/21.11130/00-1735-0000-0005-1412-0.
Повний текст джерелаPhan, Thi Thanh Hoai. "Investigating the Role of Arabidopsis Plasma Membrane Intrinsic Protein AtPIP2;1 in Seed Germination." Thesis, 2021. https://hdl.handle.net/2440/135687.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2022
Hütter, Michael [Verfasser]. "Rolle von CACNA1A, ATP1A2 und SCN1A für die sporadische hemiplegische Migräne / vorgelegt von Michael Hütter." 2009. http://d-nb.info/994819579/34.
Повний текст джерелаShearer, Monique Kirsten. "Characterisation of AtPQL1, AtPQL2 and AtPQL3 as candidate voltage insensitive non-selective cation channels (vi-NSCCs)." Thesis, 2013. http://hdl.handle.net/2440/83638.
Повний текст джерелаThesis (Ph.D.) -- University of Adelaide, School of Agriculture, Food and Wine, 2013
Friml, Jirí [Verfasser]. "Isolation and characterization of novel AtPIN genes from Arabidopsis thaliana L. / by Jirí Friml." 2000. http://d-nb.info/961738510/34.
Повний текст джерелаTurek, Ilona. "Elucidation of the Signal Transduction Pathways Activated by the Plant Natriuretic Peptide AtPNP-A." Diss., 2014. http://hdl.handle.net/10754/335803.
Повний текст джерелаOttenschläger, Iris [Verfasser]. "Gravity regulated differential auxin transport in Arabidopsis roots and the search for interaction partners of AtPIN1 / vorgelegt von Iris Ottenschläger." 2002. http://d-nb.info/964941848/34.
Повний текст джерелаDel, Vecchio HERNAN. "Biochemical and Molecular characterization of AtPAP25, a novel cell wall-localized purple acid phosphatase isozyme upregulated by phosphate-starved Arabidopsis thaliana." Thesis, 2012. http://hdl.handle.net/1974/7448.
Повний текст джерелаThesis (Master, Biology) -- Queen's University, 2012-09-10 08:28:21.631