Дисертації з теми "Atomic Force Microscopy imaging"
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Grimble, Ralph Ashley. "Atomic force microscopy : atomic resolution imaging and force-distance spectroscopy." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312277.
Повний текст джерелаLAU, JOAN M. "IMAGING MEMBRANE PROTEINS USING ATOMIC FORCE MICROSCOPY TECHNIQUES." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022192720.
Повний текст джерелаMuys, James Johan. "Cellular Analysis by Atomic Force Microscopy." Thesis, University of Canterbury. Electrical and Computer Engineering, 2006. http://hdl.handle.net/10092/1158.
Повний текст джерелаJeong, Younkoo. "HIGH SPEED ATOMIC FORCE MICROSCOPY." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1236701109.
Повний текст джерелаAlkassem, Hasan. "Imaging antimicrobial peptides in action by atomic force microscopy." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10043141/.
Повний текст джерелаHernandez, Sergio Santos. "Dynamic atomic force microscopy and applications in biomolecular imaging." Thesis, University of Leeds, 2011. http://etheses.whiterose.ac.uk/1910/.
Повний текст джерелаForchheimer, Daniel. "Imaging materials with intermodulation : Studies in multifrequency atomic force microscopy." Doctoral thesis, KTH, Nanostrukturfysik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-159689.
Повний текст джерелаSvepkraftmikroskop (eller atomkraftmikroskop från engelskans atomic forcemicroscope, AFM) är ett instrument för att avbilda ytor på mikro- och nanometer skalan. Mikroskopet känner av kraften som verkar mellan en yta och en spetsplacerad längst ut på ett mikrometerstort fjäderblad och kan därigenom skapa en topografisk bild av ytans form. Bildkontrast uppstår dock inte bara från ytans form utan även från variation i material. Förbättrad materialkontrast och förbättrad tolkning av denna kontrast är två centrala mål i vidareutvecklingen av AFM. Denna avhandling berör dynamisk AFM där fjädern drivs med flera frekvensersamtidigt. På grund av det ickelinjära förhållandet i yt-spets-kraften som funktion av spetsens position så kommer fjädern inte bara att svänga på de drivna frekvenserna utan också på övertoner och blandfrekvenser, så kallade intermodulationsprodukter. Vi undersöker primärt Intermodulation AFM (ImAFM) som ämnar att utnyttja intermodulationsprodukter nära fjäderns resonansfrekvens. Med en lämplig drivsignal genereras många intermodulationsprodukter nära resonansen, där de kan mätas med bra signal till brus förhållande. ImAFM utförs på ytor bestående av två distinkta domäner av olika material ochen kontrastmetrik introduceras för att kvantitativt utvärdera bilderna som skapas vid varje frekvens. Trots att känsligheten för kraftmätningen är högst på resonans-frekvensen, så fann vi att svaga intermodulationsprodukter bortanför resonansen kan visa hög materialkontrast. Detta resultat visar att intermodulationsbilderna kan användas för att bättre särskilja olika material. Vi har utvecklat en metod för att rekonstruera yt-spets-kraften från multifrekventa AFM spektra genom modellanpassning i frekvensrymden. Tillsammans med ImAFM leder detta till högupplösta bilder av materialparametrar. Metoden är generell och är applicerbar för olika kraftmodeller och AFM-varianter. Parametrarna har en direkt fysikalisk tolkning och, om lämpliga modeller används, kan egenskaper så som materialets elasticitetsmodul mätas. Metoden har testats på simulerat såvälsom experimentellt data, och den har också används för att särskilja topografisk kontrast från materialkontrast.
QC 20150209
Burns, Jonathan. "High resolution atomic force microscopy imaging of living bacterial surfaces." Thesis, University of Sheffield, 2017. http://etheses.whiterose.ac.uk/19929/.
Повний текст джерелаKhan, Z. "Imaging biomolecules using frequency modulation atomic force microscopy in liquids." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1399519/.
Повний текст джерелаTien, Szu-Chi. "High-speed nano-precision positioning : theory and application to AFM imaging of soft samples /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/7089.
Повний текст джерелаRodriguez, Raúl D. "Dynamic atomic force microscopy on hematite nanoparticules : beyond topographic measurements." Paris 6, 2008. http://www.theses.fr/2008PA066505.
Повний текст джерелаDans cette thèse, des études de microscopie à force atomique en mode tapping ont été effectuées sur un système de nano-particules d'hématites sur substrat de MoS2 (molybdénite) ou de SiO2 (silice). La nature des facettes des nano-particules a été déterminée en combinant à la microscopie à force atomique la microscopie électronique en transmission. Ensuite ces nano-particules et leurs substrats ont été utilisées comme système modèle pour sonder comment interroger les propriétés de surface par spectroscopie et microscopie à force atomique, au-delà de la simple information de topographie, à partir de la détermination des interactions entre la pointe d'AFM et la surface des échantillons. Les interactions de Van der Waals à longue distance et la constante de Hamaker ont été déterminées. Les interactions de contact en présence et en absence d'humidité ont été mises en évidence, menant, entre autres, à la détermination du caractère hydrophobe des nano-particules
Vuong, Ngoc Quang. "Imaging the Nicotinic Acetylcholine Receptor in Reconstituted Membranes by Atomic Force Microscopy." Thesis, University of Ottawa (Canada), 2010. http://hdl.handle.net/10393/28592.
Повний текст джерелаLu, Qingyou. "Magnetic force microscopy of colossal magneto-resistive materials and superconductors /." Digital version:, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p9992856.
Повний текст джерелаFan, Tai-Hsi. "Fluid mechanics and bio-transport phenomena in imaging of biological membranes using AFM-integrated microelectrode." Diss., Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164638/unrestricted/fan%5Ftai-shi%5F200312%5Fphd.pdf.
Повний текст джерелаCallaghan, Fergal Dominique. "Magnetic force microscope for imaging fluxlines in superconductors." Thesis, Queen's University Belfast, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287399.
Повний текст джерелаFarnham, Rodrigo Bouchardet. "Processing and inpainting of sparse data as applied to atomic force microscopy imaging." California State University, Long Beach, 2013.
Знайти повний текст джерелаGao, D. "Modeling of adsorption and atomic force microscopy imaging of molecules on insulating surfaces." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1468925/.
Повний текст джерелаSingjai, Pisith. "Improved meso-scale and atom-scale imaging for atomic force microscopy study of corrosion." Thesis, University of Surrey, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.324162.
Повний текст джерелаParlak, Zehra. "Quantitative imaging of subsurface structures and mechanical properties at nanoscale using atomic force microscope." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/37181.
Повний текст джерелаPrempraneerach, Pradya 1975. "Color imaging segmentation for automatic alignment of Atomic Force Microscope." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/89326.
Повний текст джерелаRoy, Chowdhury Susovan. "Single-Molecule Force Manipulation and Nanoscopic Imaging of Protein Structure-Dynamics-Function Relationship." Bowling Green State University / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu162707900722617.
Повний текст джерелаBudde, Michael [Verfasser]. "Single molecule force spectroscopic investigation of the siloxane bond in polydimethylsiloxane and atomic force microscopy Imaging of nucleosomal arrays / Michael Budde." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2015. http://d-nb.info/1076083897/34.
Повний текст джерелаMarutschke, Christoph Peter [Verfasser]. "Three-dimensional imaging of the solid-liquid interface with high-resolution atomic force microscopy / Christoph Peter Marutschke." Mainz : Universitätsbibliothek Mainz, 2016. http://d-nb.info/1121241832/34.
Повний текст джерелаOral, Hasan Giray. "Modeling time-resolved interaction force mode AFM imaging." Thesis, Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/43691.
Повний текст джерелаAl-Hammood, Orooba [Verfasser]. "Nano-indentation and Nano-scale imaging using atomic force microscopy: from single cell to single molecule / Orooba Al-Hammood." Bielefeld : Universitätsbibliothek Bielefeld, 2021. http://d-nb.info/1235664252/34.
Повний текст джерелаKlash, Abdalah. "Imaging and quantifying the different crystalline structures of polypropylene with the atomic force microscope." Thesis, Link to the online version, 2006. http://hdl.handle.net/10019/1070.
Повний текст джерелаWu, Ying. "Inversion-based feedforward-feedback control theory and implementation to high-speed atomic force microscope imaging /." [Ames, Iowa : Iowa State University], 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3396982.
Повний текст джерелаBippes, Christian Alexander. "Investigation of biological macromolecules using atomic force microscope-based techniques." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2009. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-23734.
Повний текст джерелаBergstrom, Torbjorn S. "Investigation of measurement artifacts introduced by horizontal scanning surface profiling instruments." Link to electronic thesis, 2002. http://www.wpi.edu/Pubs/ETD/Available/etd-0108102-131441.
Повний текст джерелаOestreicher, Zachery Walter John. "Magnetotactic Bacteria: Isolation, Imaging, and Biomineralization." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354146141.
Повний текст джерелаBerthold, Tobias. "Advanced nanoscale characterization concepts for copper interconnection technologies." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/456236.
Повний текст джерелаFor the implementation of a direct copper-copper interconnection technology, the different properties of copper (Cu), especially the oxidation behavior, impede the easy transition to Cu compared to standard materials such as aluminum or gold. Since Cu is subject to oxidation, even at room temperature, the characterization of the Cu surface is an important aspect for the process development. A novel method to research the oxidation behavior of the Cu surface in the nanoscale was developed by using combined characterization techniques. Characteristic values of the Contact Potential Difference (CPD) were obtained for the copper oxide states. By this means, Peakforce Kelvin Probe Force Microscopy (PF-KPFM) enabled to distinguish between the different types of Cu oxide with nanometer resolution and to correlate the oxidation states to local topography features. Beside the nanoscale characterization of the Cu surface, novel passivation layer in the nanometer range were introduced to achieve reliable and stable surface conditions without limiting the ability for interconnection processes. For advanced atomic force microscopy (AFM) investigations of chemical surface modifications or very soft organic protective coatings, the AFM probe tip needs to be operated in a liquid environment. The presented numerical model is able to provide accurate predictions of the drag forces present in AFM fluid imaging applications. It could be shown that triangular cantilevers provide significant lower drag forces. The influence of different liquids such as ultrapure water or an ethanol-water mixture as well as the temperature induced variation of the drag force could be demonstrated. Studies showed that thin organic Self-Assembled Monolayer (SAM) act as effective barrier to protect Cu from corrosion. The numerical model improved the AFM fluid measurements and enabled the nanoscale characterization of the CH3-terminated SAM film protecting the Cu surface. Torsional Resonance Tunneling AFM (TR-TUNA) and dynamic Chemical Force Microscopy (dCFM) enabled the correlation of high hydrophobicity and low tunneling current on nanometer scale with intact film integrity and vice versa. Compared with additional analyses, high current and low hydrophobicity could be assigned to local SAM film disintegration and local oxidation of the Cu surface at 100 °C. 150 °C finally leads to a complete decomposition of the SAM film. In addition to SAM films, the protective effect of platinum (Pt) and carbon (C) based films deposited onto Cu surfaces was reported by combined non-destructive Scanning Electron Microscopy (SEM) techniques and PF-KPFM. A C film provides a much better protective effect than a Pt layer. Besides very local sporadically distributed Cu oxide grains, a gradual degradation of the C film was not observable for a temperature up to 200 °C and layer thicknesses down to 3 nm. In contrast, the 10 nm Pt protected Cu surface exhibits already at a temperature of 150 °C locally grown Cu oxide grains. The C film passivated Cu surface has the potential of being a key technique for a reliable Cu-Cu wire bonding. Beside the research of the Cu pad surface, the Cu free air ball (FAB) formation in the ambient environment was investigated by using SEM based characterization techniques. Topographic changes of FABs with various diameters could be assigned to different oxidation layers which were well below a thickness of 55 nm. Element mappings of cross sectioned FABs showed that the oxidation occurs only on the surface. A finer grain structure and a lower grain size could be achieved by lower discharge voltages. In contrast, a lower dislocation density at the borders could be detected for higher EFO voltages. The heat transfer up to the wire and the convective cooling by the surrounding air could explain the introduced conclusions regarding the oxidation and the dislocation density.
Conneely, Michael. "High-speed imaging of holographically trapped microbubble ensembles stimulated by clinically relevant pulsed ultrasound." Thesis, University of Dundee, 2014. https://discovery.dundee.ac.uk/en/studentTheses/37b78423-14a9-4d5a-bdf1-e2a3562c66f0.
Повний текст джерелаSaxena, Shubham. "Nanolithography on thin films using heated atomic force microscope cantilevers." Thesis, Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-08302006-223629/.
Повний текст джерелаLiu, Zhen. "Reconstruction and Control of Tip Position and Dynamic Sensing of Interaction Force for Micro-Cantilever to Enable High Speed and High Resolution Dynamic Atomic Force Microscopy." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1483629656167247.
Повний текст джерелаWang, Congzhou. "INTEGRATED NANOSCALE IMAGING AND SPATIAL RECOGNITION OF BIOMOLECULES ON SURFACES." VCU Scholars Compass, 2015. http://scholarscompass.vcu.edu/etd/4067.
Повний текст джерелаMoreno, Villavicencio Maiglid Andreina. "Development of 3D high-resolution imaging of complex devices by the correlation of ToF-SIMS and AFM." Thesis, Lyon, 2019. http://www.theses.fr/2019LYSEI122.
Повний текст джерелаThe continuous miniaturization and complexity of devices have pushed existing nano-characterization techniques to their limits. The correlation of techniques has then become an attractive solution to keep providing precise and accurate characterization. With the aim of overcoming the existing barriers for the 3D high-resolution imaging at the nanoscale, we have focused our research on creating a protocol to combine time-of-flight secondary ion mass spectrometry (ToF-SIMS) with atomic force microscopy (AFM). This combination permits the correlation of the composition in 3-dimensions with the maps of topography and other local properties provided by the AFM. Three main results are achieved through this methodology: a topography-corrected 3D ToF-SIMS data set, maps of local sputter rate where the effect of roughness and vertical interfaces are seen and overlays of the ToF-SIMS and AFM advanced information. The application fields of the ToF-SIMS and AFM combined methodology can be larger than expected. Indeed, four different applications are discussed in this thesis. The procedure to obtain the topography-corrected 3D data sets was applied on a GaAs / SiO2 patterned structure whose initial topography and composition with materials of different sputter rates create a distortion in the classical 3D chemical visualization. The protocol to generate sputter rate maps was used on samples with structured and non-structured nano-areas in order to study the possible ToF-SIMS sputtering artefacts, especially the geometric shadowing effect. Finally, we have explored the combination of ToF-SIMS analysis with three AFM advanced modes: piezoresponse force microcopy (PFM), scanning capacitance microscopy (SCM) and scanning spreading resistance microscopy (SSRM). Specifically, two main applications were studied: the chemical modification during electrical stress of a piezoelectic thin film and the recovery of initial electrical characteristics of a sample subjected to Ga implantation during FIB preparation. Technical aspects of the methodology will be discussed for each application and the perspectives of this combination will be given
Zhao, Yue. "Self-Assembled Lipid Tubules: Structures, Mechanical Properties, and Applications." Doctoral diss., University of Central Florida, 2007. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2204.
Повний текст джерелаPh.D.
Department of Mechanical, Materials and Aerospace Engineering;
Engineering and Computer Science
Materials Science & Engr PhD
Zuttion, Francesca. "Effet inhibiteur des glycoclusters dans l'adhésion bactérienne des Pseudomonas aeruginosa caractérisé par microscopie à force atomique : de la molécule à la cellule." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSEC031/document.
Повний текст джерелаPseudomonas aeruginosa (PA) is a human opportunistic pathogen responsible for 20% -30% of nosocomial infections in French hospitals. For healthy people, it presents no real danger, but for people with cystic fibrosis disease and immune-compromised patients, it is the leading cause of mortality and lung infections. PA has developed antibiotic multi-resistant strains and new and more effective therapeutic approaches are needed. It binds to the surface of the host cells by an interaction between proteins (lectins) present on the membrane and sugars of the host-cell membrane. The lectin-sugar interaction plays an important role in adherence of the bacteria and in the manufacture of a pathogenic biofilm.A new therapeutic approach is to create synthetic molecules (glycoclusters) of greater affinity than the natural sugars present on the cells. To this aim, more than 150 glycoclusters have been synthetized and screened to find the best candidate to inhibit the bacteria infection process. Some of them have been selected and studied by Atomic Force Microscopy (AFM). In particular, this thesis is devoted to study the lectin-glycocluster and cell-bacteria interactions by AFM. The combination of AFM imaging with molecular dynamic simulations let understanding the role of the geometry of the glycoclusters on the complex formation, while AFM spectroscopy accesses the lectin-glycocluster interaction forces at the molecular and cellular levels. The reduction of bacterial adhesion has been observed upon the addition of the glycocluster. This confirms the anti-adhesive properties of the glycocluster and validates the procedure. The ultimate goal is the identification of the best glycoclusters in order to develop new drugs
Curry, Nathan. "Development and application of correlative STED and AFM to investigate neuronal cells." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/274579.
Повний текст джерелаValencia, Carolina Elisa Guillen. "Sistema de análise de imagens SEBS por microscopia de força atômica." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-05062014-092420/.
Повний текст джерелаIn this work, we intend to characterize the morphology of polymer thin films by techniques of image processing, mainly using computational geometry and pattern classification. The main objectives were to quantify the geometrical structures observed in thin films and describe surface patterns formed in these films. Were studied images obtained by atomic force microscopy (AFM) of SEBS [polystyrene-poly(ethylene-co-butylene)-polystyrene] thin films samples, deposited on a mica substrate by dip-coating technique . SEBS thin film polymers have great interest due to the formation of self-organized structures on the nanometer scale. The characterization and obtaining measurements of the morphology of the thin films are of relevance in this work, because they contribute to the understanding of the formation dynamics of these patterns in nanostructures studied. We analyzed different morphologies, such as droplets form with concentric rings and stripe and regularly spaced points forms. The results allow to characterize the observed patterns.
Pendery, Joel S. "Nanoscale Patterning and Imaging of Liquid Crystals and Colloids at Surfaces." Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1396623443.
Повний текст джерелаRodrigues, Carlos Alberto. "Aplicações de processamento e análise avançada de imagens para a caracterização de imagens de microscopia de força atômica." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-19102011-102450/.
Повний текст джерелаThis thesis address the application of advanced techniques of processing and analysisof images in original problems involving images of atomic force microscopy. For this, a series of algorithms for characterization and understanding of process of formation of new polymeric materials was developed and implemented, with perspectives of many technological applications. The analysis was applied to the determination of orientation of the morphology of substrates for alignment of liquid crystals, counting and estimative of radiuses of granules in layer-by-layer polymer films, analysis of enhancement of photoluminescence in PPV cast films, as well as the study of curvature spontaneous of macromolecules. The principal algorithms included are determination of inclination of eigenvectors of matrix of covariance of coordinates of points of shape,application of regional maxima technique and Voronoi diagrams, passband filters 2D through Fourier Transform and curvature multiscale. The implementation of these algorithms involved a series of image analysis algorithms such as squeletonization, exact dilations and extracting of contour of shapes. The principal contribution of this work was to develop a software called SPIA (Scanning Probe Image Analysis) that includes tools for analysis of processing of images including that were used in this work. This software was developed in Delphi under object orientation paradigm to Windows NT/9X/2000/XP. It has a friendly interface similar to other image processing softwares. All this techniques were tested extensively and the results that corroborate the robustness of the algorithms are included throughout the thesis
Obeid, Sameh. "Analyse quantitative et qualitative sur puce de vésicules extracellulaires en milieux complexes au sein d'une plateforme nanobioanalytique." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCD009/document.
Повний текст джерелаExtracellular vesicles (EVs) are small vesicles (30 to 1000 nm) released from different cell types, upon activation or apoptosis, and present in most body fluids (Blood, Urine….). Based on the current state of knowledge of their biogenesis and biochemical properties, EVs can be devided into three distinct populations: exosomes (EXO), microparticles (MPs) and apoptotic bodies (APOb). EVs have been found to play important biological roles and are also biomarkers of different pathologies. […] The first step consists of the injection of the samples containing EVs onto the biochip surface. This step is accomplished by SPR technique that allows label-free monitoring of EVs immunocapture onto the surface of a biochip presenting different specific bioreceptors. Following the capture of EVs, a nanometrological investigation of the biochip surface by AFM is engaged to characterize the physical properties of captured vesicles (size, morphology, etc..). Owning a nanometrical resolution, AFM can discriminate between individual EVs and vesicles or protein aggregates, leading to an accurate characterization of individual vesicles. The coupling of SPR technique with AFM was adapted to offer a representative global view of each array of bioreceptors and to measure the size of thousands of individual EVs. A proteomic investigation was also engaged to characterize the proteomic compositions of the different subpopulations of EVs. Such an investigation could contribute to the understanding of EVs biogenesis, biology and pathophysiology. To evaluate the potential of our platform to detect, quantify and characterize nanoparticles, two calibration particles, which cover the lower and upper size range of EVs, were chosen: (i) virus-like particles of 50 nm of diameter, also called CP50, and (ii) protein-functionnalized synthetic beads of 920 nm of diameter, called CP920. The capture tests in SPR showed a specific capture of these two calibration particles with their specific bioreceptors, immobilized onto the biochip surface, regardless the complexity of the media in which they were diluted. Also, a positive correlation was obtained between the capture level, measured by SPR, and the particle 9
Neumann, Martin. "Time-resolved imaging of the micro-mechanical behavior of elastomeric polypropylene." Doctoral thesis, Universitätsbibliothek Chemnitz, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:ch1-qucosa-182113.
Повний текст джерелаBernaud, Julien. "Propriétés physiques de capsides virales étudiées à l'échelle du virus unique par microscopie à force atomique : exemples du rétrovirus VIH-1 et du parvovirus AAV." Thesis, Lyon, École normale supérieure, 2015. http://www.theses.fr/2015ENSL1028/document.
Повний текст джерелаViruses are nanometer size biological parasite, which highjack the cellular machinery of the infected cells to replicate and thereby produce new viruses. A virus consists of a protein capsid, protecting the viral genome, a long polymer of DNA or RNA, and in some cases is surrounded by a lipid envelope. Recent work suggests that the physical properties of viruses are important in order to understand the viral cycle. In order to link the biological behavior of the virus to their physical properties, we used an approach combining AFM imaging and mechanical measurements at the nanometer scale, in connection with the physical modeling of viral capsids. We have developed automated image and force curves analysis tools to quantify the physical properties of viral capsids and the effect of the microenvironment. We have focused on two very different viruses: the HIV-1 retrovirus, responsible for AIDS and the AAV vector used in gene therapy. This work has led to the characterization of the morphological and mechanical properties of virus-like particles and cores of HIV-1 at the single virus level and on populations of hundreds of viruses. Focusing on the effect of the nature of the RNA encapsidated inside the viral particles in cellulo, we have highlighted the structural control of the viral RNA, and more precisely the psi packaging signal, on both HIV-1 VLPs and cores. Finally, we have initiated the study of the effect of reverse transcription (conversion of viral genomic RNA into DNA) within the cores HIV-1 on its stability. The study of parvovirus AAV existing form of several natural variants (serotypes) allowed us to compare the capsid physical properties at thermodynamic equilibrium and out of equilibrium. By varying the microenvironment (temperature and pH), we probed its influence on the stability of the AAV capsid. We have shown in particular that the AAV8 virus is stiffer than AAV9 while thermal stability is reduced, in relation to different biological properties for these two serotypes. In addition, the rigidity of AAV8 capsids decreases in an acidic environment mimicking the late endosome transport, and this results in a higher thermal stability. Finally, we quantified the effect of the length and nature of the confined genome on the thermal stability of AAV capsids
Faes, Romain. "Nanotubes de carbonne ultracourts pour la bioimagerie." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0035/document.
Повний текст джерелаThis thesis reports the achievement of ultrashort carbon nanotubes and their biofunctionalization for applications as near-infrared biomarker. Dispersions of carbon nanotubes in aqueous media have been formulated with various surfactants. Oxidizing chemical treatments combined with the application of ultrasounds allowed significant shortening of the carbon nanotubes. Sorting and selection of the shortest nanotubes was done by density gradient ultracentrifugation. The different fractions selected at the end of this process have been characterized by Raman spectroscopy, UV-vis absorption spectroscopy and atomic force microscopy. Selection of nanotubes of a length below 20 nm is demonstrated. We also show functionalization by antibodies and the visualization of ultrashort functionalized nanotubes by photothermal heterodyne imaging. Promising results were obtained with the specific binding of ultrashort carbon nanotubes to cells. This work open route towards bioimaging applications and in particular towards the study of the synapsis plasticity within alive neurons
Carnally, Stewart Antoni Michael. "Carbon nanotube atomic force microscopy." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491631.
Повний текст джерелаDuan, Junbo. "Restauration et séparation de signaux polynômiaux par morceaux. Application à la microscopie de force atomique." Thesis, Nancy 1, 2010. http://www.theses.fr/2010NAN10082/document.
Повний текст джерелаThis thesis handles several inverse problems occurring in sparse signal processing. The main contributions include the conception of algorithms dedicated to the restoration and the separation of sparse signals, and their application to force curve approximation in Atomic Force Microscopy (AFM), where the notion of sparsity is related to the number of discontinuity points in the signal (jumps, change of slope, change of curvature).In the signal processing viewpoint, we propose sub-optimal algorithms dedicated to the sparse signal approximation problem based on the l0 pseudo-norm : the Single Best Replacement algorithm (SBR) is an iterative "forward-backward" algorithm inspired from existing Bernoulli-Gaussian signal restoration algorithms. The Continuation Single Best Replacement algorithm (CSBR) is an extension providing approximations at various sparsity levels. We also address the problem of sparse source separation from delayed mixtures. The proposed algorithm is based on the prior application of CSBR on every mixture followed by a matching procedure which attributes a label for each peak occurring in each mixture.Atomic Force Microscopy (AFM) is a recent technology enabling to measure interaction forces between nano-objects. The force-curve analysis relies on piecewise parametric models. We address the detection of the regions of interest (the pieces) where each model holds and the subsequent estimation of physical parameters (elasticity, adhesion forces, topography, etc.) in each region by least-squares optimization. We finally propose an alternative approach in which a force curve is modeled as a mixture of delayed sparse sources. The research of the source signals and the delays from a force-volume image is done based on a large number of mixtures since there are as many mixtures as the number of image pixels
Konopinski, D. I. "Forensic applications of atomic force microscopy." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1402411/.
Повний текст джерелаAcosta, Mejia Juan Camilo. "Atomic force microscopy based micro/nanomanipulation." Paris 6, 2011. http://www.theses.fr/2011PA066691.
Повний текст джерела