Дисертації: "Associated surface form"

1

McCarthy, Erik L. "Selective Biomolecular Layers for Surface-associated Rolling Circle Amplification." Fogler Library, University of Maine, 2006. http://www.library.umaine.edu/theses/pdf/McCarthyEL2006.pdf.

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2

Tennant, Ian. "Antibody-based strategies for identifying novel apoptotic-cell surface-associated molecules." Thesis, University of Edinburgh, 2005. http://hdl.handle.net/1842/29395.

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Defective clearance of apoptotic cells (ACs) is linked to autoimmune and inflammatory disease states such as systemic lupus erythematosus and cystic fibrosis. Relatively few markers exist for the ‘eat me’ signals displayed on the AC surface despite the great potential for such molecules as diagnostic or therapeutic reagents. In this work various antibody-based strategies were employed in an attempt to identify novel AC-specific epitopes. An initial strategy utilised a phage displayed antibody library containing a repertoire of ~10⁸ antibody fragments encoded by human germline genes as an unbiased source of binding specificity. An alternative approach was based on the knowledge that receptors used by macrophages to recognise ACs also recognise pathogen-associated molecules. By looking for the ability of antibodies raised against pathogens to cross-react with ACs the hypothesis that cells undergoing apoptosis reveal molecular patterns that resemble those on pathogen related structures was tested. Screening of antibodies raised in vivo, that have previously been characterised as having specificity for pathogen-associated molecular patterns (PAMPs) revealed that some cross-react with cells undergoing apoptosis. One of these antibodies was found to bind an epitope found on the ubiquitously expressed ~40KDa precursor to Laminin-Binding-Protein (LBP/p40). These findings suggest that epitopes resembling PAMPs appear on the surface of mammalian cells as a result of apoptosis and that these epitopes can be found on endogenously expressed molecules which are normally excluded from the surface of viable cells. The ability of host receptors to cross-react with host and pathogen-associated epitopes in this way may lead to a greater understanding of the mechanisms of autoimmune reactions and allow design of approaches to stimulate the immune system for the treatment of cancer.

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3

Hathaway, Hollie. "Biomodification of abiotic surfaces for the prevention of hospital-associated infection." Thesis, University of Bath, 2017. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.760935.

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This research aims to investigate the application of medical biotechnology with regard to the detection and treatment of hospital-associated infection. Various strategies focused on the implementation of biological detection agents and biotherapeutics are discussed, with particular emphasis placed on surface-anchoring technologies. This thesis is presented in the alternative format, consisting of published research papers embedded within the text. An extended introduction precedes each publication and a reflective commentary accompanies each research paper. For the purpose of continuity, all figure captions and references are in keeping with the body of the text. Part A concerns the detection of residual sources of transmissible infection, via the utilisation of an enzymatic detection agent, capable of modelling clinical surface contamination post sterilisation. Chapter 1 details the development of a current clinical biosensor, focused on modification of polymeric substrates via plasma activation. Covalent immobilisation facilitated enhanced proteinaceous surface retention, alongside retention of biological activity, potentially providing a more stringent assessment of hospital sterilisation measures. Chapter 2 (unpublished) investigates the possibility of protein engineering for further development of the aforementioned biosensor. This research focuses on genetic modification of the detection agent and subsequent covalent conjugation to a fluorescent reporting system, with the intention of quantifying surface contamination in the clinical setting. Part B focuses on the development of potential biological therapeutics for the treatment of infection. Chapter 3 aims to provide the theoretical background to the proceeding research in the form of a review paper. Chapter 4 concerns the utilisation of surface-anchored, polymeric nanoparticles as delivery vectors for bacteriophage, facilitating the controlled delivery of the antimicrobial cargo at an elevated temperature associated with chronic wound infection. Chapter 5 is presented as an extension of the preceding research, detailing the use of a synergistic enzybiotic cocktail as oppose to bacteriophage, in an attempt to alleviate certain regulatory concerns.

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4

PEIXOTO, ARUQUIA BARBOSA MATOS. "AN APPROXIMATION SCHEME FOR IMPLICIT SURFACES BY REDENING THE ASSOCIATED OCTREE POINTS." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2013. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=37195@1.

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PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO
Neste trabalho é apresentado um método adaptativo de poligonalização de superfícies implícitas, a Grade Adaptativa, associado a uma octree como estrutura de dados. Os pontos da subdivisão dos cubos dessa octree não estão sempre no centro, como na definição clássica, porém mais próximos à superfície. Para obter essa característica, os valores da função são considerados na região de poligonalização. Os pontos da malha que aproximam a superfície são conectados utilizando as arestas dos cubos referentes às folhas dessa octree. As arestas pertencentes à interseção de três ou quatro cubos são testadas com relação a sua intersecção pela superfície. Elas são obtidas por meio de uma redefinição do conceito de Arestas Mínimas. O método apresentado nesta tese conduz a resultados mais precisos que aqueles obtidos com métodos em que a octree tenha sua subdivisão sempre no centro, como o Dual Contouring. O melhor posicionamento da grade torna possível captar mais detalhes com o mesmo nível de subdivisões, pois nesse caso há mais precisão no posicionamento dos vértices da malha e, além disso, mais cubos intersectam a superfície, gerando mais pontos da malha.
This work presents an adaptive method of polygonization for implicit surfaces, the Adaptive Grid, associated to an octree as a data structure. The octree cubes subdivision points are not always set in the center, as in the classical definition, but closer to the surface. In order to obtain this property, the values of the function are considered in the region of polygonization. The mesh points that approximate the surface are connected using the cubes edges related to the leaves in the octree. The edges that belong to the intersection of three or four cubes are tested regarding to its intersection with the surfaces. They are obtained by a redefinition of the Minimal Edges algorithm. The method presented in this work leads to more precise results than the methods for which the octree has its subdivision always in the center, as Dual Contouring. The better positioning of the grid allows to capture more details with the same subdivision level, because in this case there is more accuracy in the mesh vertex positioning and moreover, more cubes intersect the surface, generating more mesh points.

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5

A, Abdul Malik Shareen. "Defence on surface of Rhodophyta Halymenia floresii : metabolomic profile and interactions with its surface-associated bacteria." Electronic Thesis or Diss., Lorient, 2020. http://www.theses.fr/2020LORIS598.

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Halymenia floresii, une Rhodophycée présente une surface remarquablement exempte d'épiphytes dans les conditions de l'Aquaculture MultiTrophique Intégrée (AMTI). Ce phénomène traduit la présence potentielle en surface de composés actifs allélopathiques. L'objectif de ce travail a été d'explorer les mécanismes de défense développés par H. floresii contre l'épibiose, de détecter et d'identifier les métabolites secondaires produits à la surface de l’algue et d'étudier les relations avec les bactéries épiphytes. Nous avons ainsi pu isoler la communauté épibactérienne de H. floresii cultivée dans des conditions contrôlées (AMTI) et non contrôlées (échantillons collectés in situ). Les épibactéries isolées ont été criblées in vitro pour analyser les signaux de détection de Quorum Sensing (QS). Les extraits produits en surface ont été analysés pour détecter toute interférence avec le Quorum Sensing. Les épibactéries pathogènes et non-pathogènes ont été différenciées par leur capacité à induire une maladie algale, le blanchiment. Vibrio owensii, ainsi que son signal C4-HSL QS, a été identifié comme pathogène opportuniste induisant un blanchiment. Les métabolites extraits de la surface et de cellules entières de H. floresii ont été analysés par LC-MS. Une base de données a été constituée à partir d’une analyse métabolomique non ciblée. Quarante et un métabolites actifs ont été identifiés, parmi lesquels les composés halogénés, des furanones et divers inhibiteurs étaient surreprésentés. Fait intéressant, les deux premières classes sont connues comme de puissants composés interférant avec le QS. La présence relativement élevée de métabolites allélopathiques à la surface de H. floresii soutient fortement l'hypothèse selon laquelle ils doivent être impliqués dans la protection de l'hôte. Des recherches supplémentaires seront nécessaires pour explorer l’ensemble des métabolites secondaires produits par H. floresii et leurs rôles chez l’algue
The surface of Halymenia floresii, a Mexican Rhodophyta, was observed to be remarkably free of epiphytes under Integrated MultiTrophic Aquaculture (IMTA) conditions. This suggests the presence of allelopathic active compounds released by this macroalgae. The aim of this work was to explore the defence mechanisms developed by H. floresii against surface epibiosis, to detect and identify the secondary metabolites produced at the surface of the algae, and to study its relation with surface associated bacteria. For the first time, we isolated the epibacterial community of H. floresii cultivated under controlled conditions (IMTA) and uncontrolled ones (beach-cast material collected in the area). The isolated epibacteria were screened in vitro to analyse Quorum Sensing (QS) signals, and others H. floresii surface extracts were assayed for any QS interference with them. We differentiated the epibacteria significant pathogens from the non- pathogens ones by their ability to induce bleaching, a well-known algal disease. Vibrio owensii was identified as an opportunistic pathogen inducing bleaching in H. floresii which was also associated to the presence of its C4-HSL QS signal. The surface and whole cell metabolites extracts from H. floresii specimens cultivated under controlled conditions were analysed by means of LC/MS. An untargeted metabolomic analysis of H. floresii was performed to provide a global metabolic profile as a first database. We identified ‘41’ active metabolites in H. floresii, among which halogenated compounds, furanones and various inhibitors were overrepresented. Interestingly, the first two classes are well known potent QS interfering compounds. The relatively higher occurrences of allelopathic metabolites at the surface of H. floresii strongly supports the hypothesis that they must be involved in the host protection. Further investigations are needed to explore the secondary metabolites of H. floresii et their role in the seaweed

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6

Kazemzadeh-Narbat, Mehdi. "Local delivery of antimicrobial peptides from titanium surface for the prevention of implant-associated infections." Thesis, University of British Columbia, 2013. http://hdl.handle.net/2429/44017.

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Titanium (Ti) is a key biomedical material extensively used in orthopaedic implants. Prevention of implant-associated infections has been one of the main challenges in orthopaedic surgery. This challenge is further complicated by the concern over the development of antibiotic resistance as a result of using traditional antibiotics for infection prophylaxis. One of the promising alternatives is the family of antimicrobial peptides (AMPs). The present dissertation develops progressive approaches that enable the loading and local delivery of a unique group of cationic antimicrobial peptides through titanium implant surfaces. In the first technique, a thin layer of micro-porous calcium phosphate (CaP) coating was processed by electrolytic deposition onto the surface of titanium as the drug carrier. The AMP-loaded CaP coating was not cytotoxic for MG-63 osteoblast-like cells, and the implants showed high antimicrobial activity against both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with 10⁶-fold reductions of both bacterial strains within 30 min and ∼92% and ∼77% inhibition of luminescence at 4 h and 24 h, respectively. Second study investigated the in vitro AMP release, antimicrobial performance, and cytotoxicity of a modified Tet213 (HHC36), as well as the in vivo bone growth of AMP loaded into calcium phosphate coated Ti implants in a rabbit model. Burst release during the first few hours followed by a slow and steady release for 7 days was observed. In vivo bone growth study showed that loading of AMP did not impair bone growth onto the implants. In the last study multilayer thin films of titania nanotubes (NT) and CaP coatings were formulated with AMP and were topped with a thin phospholipid film similar to cell membrane. The films were shown to be non-cytotoxic, hydrophilic, with the potential of tuning loading and release kinetics of AMP. The best model describing the AMP release was first-order model. The first two approaches demonstrated a promising method for an early stage peri-implant infection treatment. The last study proposed a technique to improve the kinetics of AMP release and total loaded AMP quantity, and to increase the Ti interfacial strength while maintain the osteconductivity by applying CaP coating.

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7

Моравецька, Катерина Вiталiївна. "Мiри на банахових многовидах з рiвномiрною структурою". Doctoral thesis, Київ, 2018. https://ela.kpi.ua/handle/123456789/26061.

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Дисертацiя присвячена диференцiйовним мiрам на банахових многовидах з рiвномiрною структурою. Запропоновано метод побудови асоцiйованих мiр на поверхнях скiнченної корозмiрностi, вкладених у банахiв многовид з рiвномiрною структурою. Введено поняття асоцiйованої диференцiальної форми поверхнi та строго трансверсального до поверхнi набору векторних полiв. Доведено теорему “про узгодженiсть”, згiдно з якою поверхнева мiра задається однозначно асоцiйованою диференцiальною формою поверхнi. Показано транзитивнiсть запропонованої конструкцiї. На прикладi мiри Лебега в скiнченновимiрному просторi Rn та мiри об’єму на рiмановому многовидi з рiвномiрною структурою обґрунтовано її адекватнiсть. Отримано узагальнення низки результатiв з теорiї диференцiйовних мiр на лiнiйних просторах на випадок банахових многовидiв з рiвномiрною структурою. Доведено критерiй слабкої диференцiйовностi мiри уздовж обмеженого векторного поля, що узагальнює вiдомий результат В. I. Богачова.

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Jordão, Nuno Ricardo de Oliveira. "Study of the cell surface proteome for the analysis of Parkinson’s disease associated DJ-1 mutations." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/15483.

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Mestrado em Biotecnologia - Biotecnologia Molecular
Parkinson’s disease (PD) is a neurodegenerative disease, characterized with selective neurodegeneration and dopamine depletion. Despite most cases appear to have sporadic origin, it has been associated various monogenic mutations to the onset of a parkinsonian phenotype. DJ-1 protein is of particular interest given its neuroprotective role against oxidative stress and mitochondria impairment, and the identification of several mutations correlated with early onset PD. For this study, it were then produced two pathological mutations of DJ-1, M26I and E163K. SDS-PAGE and LC-MS/MS analysis confirmed the adequate production and purification of the both mutant proteins, and SEC-HPLC secured the structural perseverance of the mutations as homodimers, a key feature of DJ-1 essential for its biological activity. On the other hand, SH-SY5Y viability assays indicated that despite the native form protective role against oxidative stress, M26I and E163K mutations showed a compromised neuroprotective capacity. To better understand the reasons for this biological impairment, it was developed a protocol for cell surface proteins labelling with Sulfo-NHSLC-biotin and avidin pull-down for enrichment and downstream MS analysis. Assays such as western blotting, LC-MS/MS and confocal microscopy confirmed the adequacy of the proposed procedure. When applied for the analysis of proteome variations related to oxidative stress, in enriched fractions from SH-SY5Y biotinylation and avidin pull-down of crude membrane sub cellular part, it allowed the identification of several proteins of interest, namely four proteins with significant difference caused by oxidative stress induction, and of other proteins of interest. It was also performed the direct pull-down of whole protein extract, offering inconclusive results regarding the preferential use of ultracentrifugation before pull-down. Nevertheless, it was the first time that SH-SY5Y cell surface was analysed in a PD context, and it could be used in the future to study cell surface proteome alterations modulated by oxidative stress and extracellular presence of native or mutant DJ-1, providing new insights regarding its intake and signalling modulation in pathological conditions, and hence contributing for a new perspective over preventive or eliciting mechanisms associated to the onset of Parkinson’s disease.
A doença de Parkinson é uma doença neurodegenerativa caracterizada por uma neurodegeneração selectiva e depleção de dopamina, e apesar de grande parte dos casos terem origem esporádica diversas mutações monogénicas têm sido associadas ao desenvolvimento de um fenótipo parkinsoniano. A proteína DJ-1 é de particular interesse, dado o seu papel neuroprotector contra stress oxidativo e disfunção mitocondrial, e a identificação de mutações correlacionadas a doença de Parkinson precoce. Neste estudo, foram produzidas duas mutações patológicas da proteína DJ-1, M26I e E163K. Uma análise SDS-PAGE e LC-MS/MS comprovou uma produção e purificação adequada das mutações, e SEC-HPLC assegurou a preservação estrutural das mutações de DJ-1 como homodímeros, uma característica chave de DJ-1 fundamental para a sua actividade biológica. Por outro lado, estudos de viabilidade de SH-SY5Y indicaram que, apesar do papel protector da forma nativa contra stress oxidativo, as mutações M26I e E163K demonstraram uma reduzida capacidade neuroprotectiva. Para melhor compreender os motivos desta disfunção biológica, foi desenvolvido um protocolo para marcação das proteínas de superfície celular com Sulfo-NHS-LC-biotina e pull-down com avidina para enriquecimento e subsequente análise MS. Vários ensaios como western blotting, LC-MS/MS e microscopia confocal confirmaram a adequação do protocolo sugerido. Quando aplicado para uma análise de variações proteómicas relacionadas com stress oxidativo, em fracções enriquecidas provenientes da biotinilação de SH-SY5Y e pull-down da parte membranar do extracto celular, permitiu a identificação de várias proteínas de interesse, nomeadamente quatro proteínas com diferença significativa resultante da indução de stress oxidativo. Também foi realizado um pull-down com a totalidade extracto celular, que resultou em dados não conclusivos relativamente ao uso de ultracentrifugação antes do pull-down. Não obstante, este estudo correspondeu à primeira análise da superfície celular de SH-SY5Y realizada num contexto da doença de Parkinson, que poderá ser usada no futuro para estudar alterações no proteoma de superfície celular em ambiente de stress oxidativo e de adição da proteína DJ-1, na forma nativa e mutante, de forma a fornecer novas pistas referentes ao seu intake e modulação da sinalização em ambiente oxidativo, e em suma contribuindo para uma nova perspectica sobre os mecanismos protectores ou despoletadores da doença de Parkinson.

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Vargas, Vallejo Michel Eduardo. "Nanoscale structural/chemical characterization of manganese oxide surface layers and nanoparticles, and the associated implications for drinking water." Diss., Virginia Tech, 2016. http://hdl.handle.net/10919/64514.

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Water treatment facilities commonly reduce soluble contaminants, such as soluble manganese (Mn2+), in water by oxidation and subsequent filtration. Previous studies have shown that conventional porous filter system removes Mn2+ from drinking water by developing Mn-oxides (MnOx(s)) bearing coating layers on the surface of filter media. Multiple models have been developed to explain this Mn2+ removal process and the formation mechanism of MnOx(s) coatings. Both, experimental and theoretical studies to date have been largely focused on the micrometer to millimeter scale range; whereas, coating layers are composed of nanoscale particles and films. Hence, understanding the nanoscale particle and film formation mechanisms is essential to comprehend the complexity of soluble contaminant removal processes. The primary objective of this study was to understand the initial MnOx(s) coating formation mechanisms and evaluate the influence of filter media characteristics on these processes. We pursued this objective by characterizing at the micro and nanoscale MnOx(s) coatings developed on different filter media by bench-scale column tests with simulating inorganic aqueous chemistry of a typical coagulation fresh water treatment plant, where free chlorine is present across filter bed. Analytical SEM and TEM, powder and synchrotron-based XRD, XPS, and ICPMS were used for characterization of coatings, filter media and water solution elemental chemistry. A secondary objective was to model how surface coating formation occurred and its correlation with experimentally observed physical characteristics. This modeling exercise indicates that surface roughness and morphology of filtering media are the major contributing factors in surface coating formation process. Contrary to previous models that assumed a uniform distribution and growth of surface coating, the experimental results showed that greater amounts of coating were developed in rougher areas. At the very early stage of coating formation, unevenly distributed thin films and/or particle aggregates were observed, which provided active sites for further surface coating growth. The predominant MnOx(s) phase in the surface coatings was identified to be poorly crystalline birnessite having scavenging activity by intercalation and/or sorption. This would explain the enhancement of efficiency in removing soluble manganese and other contaminants during water filtration. Moreover, the increased Mn2+ removal effect of having aluminum (Al) in pre-treated water is explained. These results indicate that the surface roughness and morphology need to be incorporated into particle capture models to more precisely describe the soluble manganese removal process.
Ph. D.

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10

Frontino, Gina Christine. "Comparison of Methods for Detection of Listeria on Wooden Shelves used for Cheese Aging: Challenges Associated with Sampling Porous Surfaces." ScholarWorks @ UVM, 2019. https://scholarworks.uvm.edu/graddis/1008.

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This thesis examined the efficacy of various sampling and detection methods used for environmental monitoring of Listeria species on wooden surfaces used for cheese aging. Government agencies including the Food and Drug Administration (FDA) and United States Department of Agriculture (USDA) recommend enrichment methods coupled with use of environmental sponges and swabs. Our study compared efficacy of sponge swabs manufactured by 3M™ and World Bioproducts. There is a lack of research validating the best performing swab type and enrichment method combination that is sensitive when used on rough, porous surfaces. The sensitivity of these environmental sampling tools and methods are critical considerations to effectively monitor the presence of Listeria species on wooden boards used during aging of artisan cheese. Seasoned spruce wooden shelves, cut into 100cm2 replicates, were spot inoculated with varying concentrations of Listeria species inocula, the Listeria species strains consisted of two L. monocytogenes strains and a Green Florescence Protein (GFP) expressing strain of L. innocua. The inoculated wooden surface was swabbed with three environmental sampling sponge/swab formats (World Bioproducts© EZ ReachTM environmental swabs (WBEZ) with HiCap (WBHC) and Dey-Engley (WBDE) neutralizing broths; and 3MTM environmental swabs (3MTM) with Dey-Engley neutralizing broth). Enumeration methods were used to determine the low target limits of detection. Once the low target concentrations were identified, five enrichment methods consisting of 3MTM Listeria Environmental Plate, FDA, Dual Enrichment, modified USDA, and modified FDA were challenged against low concentrations of Listeria species inocula (0.01 cfu/cm2, 0.1 cfu/cm2, 1 cfu/cm2) and the three environmental sponge swab formats. Performance of the swab formats was assessed by collection of naturally contaminated environmental samples (n=405) from dairy farm environments, swabbing where wooden surfaces existed, and analyzed using the most effective enrichment methods found from previous experiments. Lastly, the wooden surfaces and sponge swabs were observed under a Florescent Microscope using GFP L. innocua to visually determine how each sponge material of the 3M™ and World Bioproducts recovered the inocula. When wood surfaces were inoculated at high concentration levels of Listeria spp., all swab formats performed equally for detecting Listeria. Success of positive recovery at low concentrations was variable, where enrichment methods and swabs were not dependent on each other. The swab format that worked best for detecting low levels of Listeria species was the WBDE sponge swab. The WBDE swab also performed the best in dairy farm environmental sampling. The m-USDA enrichment method was found to be most effective in recovery and repair of low and potentially injured Listeria spp. Wooden surfaces are rough and porous and should be taken into consideration when creating an environmental sampling plan for these food contact surfaces. All swabs and methods performed with only slight variation, but the variation could be significant when monitoring wooden shelves with low level contamination of Listeria species. Artisan cheesemakers who use wooden shelves during the aging of their cheese, should ensure use of the most sensitive detection methods.

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Biddulph, Matilda. "Testing the efficacy of mitigation measures for reducing fine sediment and associated pollutant delivery to and through rivers in agricultural catchments of England." Thesis, University of Northampton, 2016. http://nectar.northampton.ac.uk/9543/.

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Agricultural intensification has contributed to the degradation of freshwaters in the UK, through enhanced delivery of fine sediment and associated contaminants, leading to sedimentation and eutrophication. European legislation (Water Framework Directive 2000/60/EC) and subsequent UK government initiatives such as Catchment Sensitive Farming (CSF) and Demonstration Test Catchments (DTC), aim to improve the quality of freshwaters. The DTC programme aims to find cost-effective ways to reduce agricultural diffuse water pollution. This study aimed to monitor rivers in the Hampshire Avon DTC with existing and planned mitigation measures, to measure the effectiveness of the mitigation measures, and to develop an experimental design for wider application for pollution mitigation. The methods used and tested in this study were a combination of affordable, replicable and sustainable methods (in-stream sediment collection and water quality monitoring), more complicated, expensive, analytical laboratory methods (particle size, loss-on-ignition, geochemistry, mineral magnetism, environmental radionuclides), and sediment source fingerprinting. The mitigation measures were: improvement to farm infrastructure, a wooded and a grassed riparian buffer, and a constructed wetland and in-stream pond treatment train. The improved farm infrastructure (resurfacing of a farm track, installation of a retention pond, improvements to a drainage ditch) effectively reduced inputs of sediment and associated contaminants to the river, however, this had little positive impact on the river due to greater importance of inputs from other sources. The riparian buffers were reducing fine sediment and associated contaminant inputs laterally and from upstream, however, the effectiveness of the riparian buffers was undermined by a lack of riparian buffers upstream and by sub-surface field drains. Combined analysis of the river from the farm infrastructure in the headwaters (farm scale) to the wooded riparian buffer downstream (sub-catchment scale) showed a change in the dominant source of sediment. This highlighted that an experimental design would require monitoring at varying spatial scales, as individual farm scale mitigation measures may have little impact on an entire sub-catchment due to the importance of other sources from a larger drainage area. The constructed wetland and instream pond were not effective at reducing longitudinal delivery of fine sediment and associated contaminants due to issues related to maintenance and design, emphasising the importance of appropriate targeting, design, and maintenance of mitigation measures. The results from this study showed that the methods used would be suitable as part of an experimental design for wider application. Although complex and expensive, sediment source fingerprinting is essential for determining appropriate and cost-effective mitigation at farm and sub-catchment scales. Monitoring of the sediment and water quality using the affordable, replicable and sustainable methods could be managed by farmers and landowners across a dense spatial area, at a high temporal frequency, to ensure sustainable effectiveness of mitigation. There is a need for more co-working between policy makers and scientists to ensure appropriate funding and timescales for research are provided, and with farmers and landowners to improve understanding and vested interest in the contribution of agriculture to the degradation of water quality.

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Munk, Peter. "A uniform geometrical theory of diffraction for the radiation and mutual coupling associated with antennas on a material coated convex conducting surface /." The Ohio State University, 1996. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487932351057845.

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13

Irani, Dilshad Minocher. "Role of the surface associated material of Eikenella corrodens in bone resorption associated with periodontal disease : a research thesis submitted in fulfilment of the requirements for the degree of Master of Science in Dentistry." Title page, contents and summary only, 1998. http://web4.library.adelaide.edu.au/theses/09DSM/09dsmi65.pdf.

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14

Moche, Martin [Verfasser]. "Optimization and application of the biotinylation approach for extraction and mass-spectrometric analysis of cell-surface associated proteins from Gram-positive bacteria / Martin Moche." Greifswald : Universitätsbibliothek Greifswald, 2016. http://d-nb.info/1105570231/34.

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König, Benno [Verfasser], Tim N. [Akademischer Betreuer] Hoffmann, Tim N. [Gutachter] Hoffmann, Johannes [Gutachter] Wallner, and Wayne [Gutachter] Rossman. "A Geometric Construction for the Associated Family of S–Isothermic CMC Surfaces / Benno König ; Gutachter: Tim N. Hoffmann, Johannes Wallner, Wayne Rossman ; Betreuer: Tim N. Hoffmann." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1166314936/34.

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16

Devanathan, Vasudharani [Verfasser]. "Cellular form of prion protein (PrPC) associates with contactin associated protein (Caspr) and inhibits its serine protease reelin-mediated shedding on the neuronal surface in mouse (Mus musculus, Linnaeus, 1758) / von Vasudharani Devanathan." 2008. http://d-nb.info/988565544/34.

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17

"Surface-immobilized adeno-associated virus nanoparticles for applications in controlled gene delivery and biosensing." Thesis, 2011. http://hdl.handle.net/1911/70344.

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Adeno-associated virus (AAV) is a 25 nm replication deficient DNA virus most commonly studied for human gene therapy applications. The work detailed in this thesis investigates the controlled delivery of AAV from surfaces for both transduction and biosensing applications. First, AAV was tested for compatibility with substrate-mediated gene delivery approaches. Two reverse transduction applications were investigated: (1) spatial localization of cells and virus vectors for tissue engineering applications and (2) live cell genetic microarrays. To drive the proper differentiation and assembly of cells within tissue engineering constructs, gene expression patterns may need to be tightly regulated. To localize adhesive proteins and AAV, polydimethylsiloxane stamps and protein adhesive alkanethiols were used. By adsorbing AAV onto adhesive proteins, including human fibronectin, laminin, collagen I, elastin and poly-l-lysine, both cell adhesion and gene delivery were localized to a defined pattern. Gene delivery was efficient on all protein surfaces, with higher expression observed on laminin surfaces. AAV was also patterned using a robotic spotter to create live cell genetic microarrays, creating localized cell islands expressing GFP. This potentially high-throughput technique could be extended to study complex genetic interactions within cells, such as stem cells or induced pluripotent stem cells. Additionally, AAV was explored as a biosensor by modifying virus output functionalities. Wild-type AAV2 externalizes an N-terminus region containing a phospholipase A2 (PLA2) domain during intracellular processing, allowing the virus to escape the endosomal pathway and deliver genetic cargo. This externalization can be replicated outside of cells through heat treatment. AAV2-ΔPLA2-His was created by replacing the PLA2 domain with a nickel binding hexahistidine tag. This replacement allows heat-treated mutant virus to bind a nickel affinity column. Finally, directed evolution was used to (1) improve the ability of AAV to deliver genes into target cells or (2) alter AAV biosensor inputs. Virus libraries were created using error-prone polymerase chain reaction (EP-PCR) to introduce random amino acid modifications into the protein capsid. The error rate for these libraries was estimated to be between 5-7 errors per cap gene. Combining surface immobilization with directed evolution could allow for precise control of AAV for gene delivery and biosensing.

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18

Lin, Li-Ching, and 林立青. "An adaptive neuro-fuzzy method associated with tide generating forces and sea surface temperature for tidal prediction." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/31405339809011100906.

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博士
國立交通大學
土木工程系所
95
The aim of this paper is to clarify how tide generating forces (TGF) and sea surface temperature (SST) affect the water level. This paper also uses back-propagation neural network (BPN) and adaptive network-based fuzzy inference system (ANFIS) to construct the tide predicting models. This study presents a neural network model, called TGF-NN, of simulating tides at multi points considering tide generating forces. The other model, called TGFT-FN, embedded tide generating forces and sea surface temperature is to calculate tides. A comparison on the root mean square and correlation coefficient of three year mixed tides at a single point computed with harmonic method, response-orthotide method, the NAO.99b model and the TGF-NN model was made to show the prediction accuracy of each method. This model is shown to be efficient as the harmonic method to estimate ocean tides at a single point. Extended application of this model to predicting tides at some points neighboring to an original interest point identifies accurately simulating multi-point tides as the NAO.99b numerical model. Furthermore, the difference of the model combined SST and TGF parameters and the TGF-NN model in eastern coast of Taiwan is about 5 cm, in southwestern coast is about 2 cm, and in northwestern coast is almost the same.

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19

Wei, Ya-Ling, and 魏雅鈴. "Modulation of immune surface molecules associated with the efficacy of antiviral therapy for chronic hepatitis C virus infection." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/74461008454444588958.

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碩士
國立成功大學
醫學檢驗生物技術學系
94
Hepatitis　C　virus　(HCV)　diseases　threaten　global　human　health.　The　current　treatment　for　chronic　HCV　infection　with　combination　regimen　of　pegylated　interferon　(IFN)-alpha　and　ribavirin　can　exert　overall　response　rate　of　approximately　46-78%,　which　is　influenced　by　viral　genotype,　initial　circulating　viral　load　and　host　factors.　Host　factors　such　as　immunological　regulators　seem　to　affect　the　outcome　of　antiviral　treatment　of　pegylated　IFN-alpha　and　ribavirin　since　both　agents　are　known　immumodulators.　Proper　antigen　presentation　function　is　required　to　elicit　sufficient　adapted　cellular　immunity　against　viral　infection,　which　has　been　impaired　in　patients　chronically　infected　with　HCV.　This　study　aims　to　investigate　whether　the　modulation　of　immuno-surface　proteins　by　pegylated　interferon　(IFN)-alpha　and　ribavirin　is　related　to　therapeutic　responses.　We　enrolled　35　patients　with　chronic　hepatitis　C,　who　have　undergone　combination　therapy.　We　longitudinally　assessed　the　effect　of　anti-HCV　agents　on　the　expression　levels　of　CD81,　HLA-ABC,　CD86　and　CD28,　the　three　major　components　in　trigger　of　antigen-dependent　and　antigen-independent　co-stimulatory　signals,　in　peripheral　blood　mononuclear　cells　(PBMCs)　from　patients　receiving　combination　therapy　for　24　weeks　and　afterward　follow-up　for　24　weeks.　FACS　analysis　showed　that　HLA-ABC　and　CD86　upregulation　on　B　cells　during　combine　therapy　in　patients　with　sustained　virological　response　(SVR)　(n=26),　but　not　in　patients　without　SVR　(n=9).　Elevation　of　surface　HLA-ABC　expression　was　not　secondary　to　increased　RNA　levels　in　B　cells.　The　treatment　induced　upregulation　of　surface　CD28　in　CD4+　and　CD8+　T　cells　in　both　of　the　patient　groups.　The　analysis　of　polymorphic　HLA-ABC　alleles　showed　no　significant　difference　in　both　patient　groups.　In　conclusion,　the　expression　levels　of　HLA-ABC　and　CD86　were　up-regulated　on　B　cells　during　combination　therapy　only　in　patients　with　SVR　but　not　non-SVR.

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20

Мелещенко, Володимир Олександрович. "Електрохімічні та аналітичні характеристики рідинного електрода з мембраною на основі нітробензену та оберненого до аніону (2-(2-додецилокси)етокси)етил сульфату". Магістерська робота, 2020. https://dspace.znu.edu.ua/jspui/handle/12345/1424.

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Мелещенко В. О. Електрохімічні та аналітичні характеристики рідинного електрода з мембраною на основі нітробензену та оберненого до аніону (2-(2-додецилокси)етокси)етил сульфату : кваліфікаційна робота магістра спеціальності 102 "Хімія " / наук. керівник О. В. Луганська. Запоріжжя : ЗНУ, 2020. 58 с.
UA : В роботі 58 сторінок, 7 таблиць, 13 рисунків було використано50літературних джерел, з них19на англійській мові. Об’єктом дослідження є аніон (2-(2-додецилокси)етокси)етил сульфату. Предметом дослідження є електродні характеристики іоноселективного електроду, оберненого до аніону (2-(2-додецилокси)етокси)етил сульфату. Метою даної роботи синтезувати електродноактивну речовину аніону лаурет сульфату та катіону метилового фіолетового; розробити ІСЕ і порівняти електродні властивості з мембранними розчинниками різної полярності; встановити кореляційні залежності між верхньою і нижньою межамивиявлення та діелектричної проникністюмембранного розчинника; створити електрод з найкращими електрохімічними характеристикамита застосувати при розробці методик кількісного визначення в реальному об’єкті «Чиста лінія». Використана апаратура : аналітичні ваги II класу, техно-хімічні ваги, хімічний посуд, рН-метр. Методи досліджень : метод прямої потенціометрії, метод градуювального графіка, метод стандартних добавок, метод подвійних стандартних добавок, метод статистичної обробки.
EN : In the work 58 pages, 7 tables, 13 drawings, 50 literature sources were used, 19 of them in English. The object of study isanion(2-(2-dodecyloxy)ethoxy)ethyl sulfate. The subject of the study is the electrode characteristics of an ionoselective electrode reversed to the anion (2-(2-dodecyloxy)ethoxy)ethyl sulfate. The purpose of this work isto obtain an electrode active substanceanionlaureth sulfateandmethyl violet; to develop ISE and compare electrode properties with membrane solvents of different polarity; establish correlations between the upper and lower limits of detection and the dielectric constant of the membrane solvent; to create an electrode with the best electrochemical characteristics and to apply in the development of methods of quantification in the real object «Clean line». Research apparatus are analytical scales of II class, techno-chemical scales, chemical vessels, pH meter. Research methodsaredirect potentiometry method, calibrationgraph method, standard additive method, double standard additive method, method of statistical processing.

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21

Мостевой, Віталій Леонідович. "Розробка іоноселективного електрода, оберненого до аніону (2-(2-додецилокси)етокси)етил сульфату з використанням трикрезилфосфату в якості пластифікатора мембрани". Магістерська робота, 2020. https://dspace.znu.edu.ua/jspui/handle/12345/1914.

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Мостевой В. Л. Розробка іоноселективного електрода, оберненого до аніону (2-(2-додецилокси) етокси) етил сульфату з використанням трикрезилфосфату в якості пластифікатора мембрани : кваліфікаційна робота магістра спеціальності 102 "Хімія" / наук. керівник О. В. Луганська. Запоріжжя : ЗНУ, 2020. 59 с.
UA : В роботі 59 сторінки, 3 таблиці, 17 рисунків, було використано 66 літературних джерел, з них 25 на англійській мові. Об’єктом дослідження є електрод з полівінілхлоридною мембраною, пластифікованою трикрезилфосфатом та оберненою до аніону (2-(2-додецилокси)етокси)етил сульфату. Предметом дослідження є електродні характеристики іоноселективного електроду, оберненого до аніону (2-(2-додецилокси)етокси)етил сульфату. Метою даної роботи є: отримати осад іонного асоціату метиловий фіолетовий – натрій (2-(2-додецилокси)eтокси)етил сульфат; розробити оптимальний склад мембрани; вивчити електродні характеристики конструйованого ІСЕ; обробити результати статистичним методом. Методи досліджень та апаратура – аналітичні ваги II класу, техно-хімічні ваги, хімічний посуд, рН - метр. Метод градуювального графіка, метод потенціометричного титрування, метод статистичної обробки.
EN : In the work 59 pages, 3 tables, 17 drawings, 66 literature sources were used, 25 of them in English. The object of study is a polyvinyl chloride membrane electrode, plasticized with tricresyl phosphate and anionic (2-(2-dodecyloxy)ethoxy)ethyl sulfate anion. The subject of the study is the electrode characteristics of an ion-selective electrode reversed to the anion (2-(2-dodecyloxy)ethoxy)ethyl sulfate. The subject of the study is the electrode characteristics of an ion-selective electrode reversed to the anion (2-(2-dodecyloxy)ethoxy)ethyl sulfate. The purpose of this work is: to obtain an ionic associate precipitate methyl violet - sodium (2-(2-dodecyloxy)ethoxy)ethyl sulfate; to develop the optimal composition of the membrane; to study the electrode characteristics of the designed ICE; process the resultsby statistical method. Research methods and apparatus - analytical scales of classII, techno-chemicalscales, chemicalvessels, pH-meter. Methods: method of calibration graph, method of potentiometric titration, method of statistical processing.

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