Дисертації з теми "Associated gene"
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Hofstra, Robert Martinus Wouter. "The RET gene and its associated diseases." [S.l. : [Groningen] : s.n.] ; [University Library Groningen] [Host], 1995. http://irs.ub.rug.nl/ppn/142201383.
Повний текст джерелаJones, Richard Julian. "Novel gene therapy for EBV-associated malignancies." Thesis, University of Birmingham, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274412.
Повний текст джерелаJames, Pamela. "Characterization of Novel Lymphoid-Associated Genes Identified by Gene-Trapping: a Dissertation." eScholarship@UMMS, 2006. http://escholarship.umassmed.edu/gsbs_diss/231.
Повний текст джерелаRead, Tara. "Elucidating a novel gene associated with myoclonus dystonia." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28248.
Повний текст джерелаGordon, Linda Anne. "Investigation of potential problems associated with gene therapy." Thesis, University of Leicester, 1998. http://hdl.handle.net/2381/30742.
Повний текст джерелаBehrens, Renee F. "Changes in endometrial gene expression associated with Infertility." Thesis, Imperial College London, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511836.
Повний текст джерелаSawhney, Namita. "Studies of the growth arrest associated gene - prohibitin." Thesis, King's College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261713.
Повний текст джерелаGlod, Frank. "PCR generated gene probes for cloning fungal polykeptide synthase genes associated with squalestatin biosynthesis." Thesis, University of Bristol, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268525.
Повний текст джерелаJohn, Christopher Robert. "Gene expression associated with the evolution of C₄ photosynthesis." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709401.
Повний текст джерелаChilds, Andrew James. "Tex19 : a germ cell-specific gene associated with pluripotency." Thesis, University of Edinburgh, 2007. http://hdl.handle.net/1842/29064.
Повний текст джерелаBandekar, Aditya C. "Cell Cycle Associated Gene Expression Predicts Function in Mycobacteria." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1068.
Повний текст джерелаMiller, Jennifer Dawn. "Senescence-associated gene expression in ozone-stressed Arabidopsis leaves." Adobe Acrobat reader required to view the full dissertation. Adobe Acrobat reader required to view the full dissertation, 2000. http://etda.libraries.psu.edu/theses/available/etd-0626100-201030/.
Повний текст джерелаAccompanied by: Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis. 1015-1023 p. : ill. (some col.) ; 28 cm. Published in Plant physiology, August, 1999, v. 120. Availabe online.
Cesar, Aline Silva Mello. "Identification of genes associated with intramuscular fat deposition and composition in Nellore breed." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11139/tde-12082014-103102/.
Повний текст джерелаA quantidade e composição da gordura intramuscular (GIM) pode influenciar as características sensoriais, o valor nutricional da carne bovina e na saúde humana. O perfil dos seus ácidos graxos pode se apresentar de maneira diversificada conforme a genética, o manejo e a nutrição dos animais de origem. A deposição e composição da gordura são determinadas por muitos genes que participam direta ou indiretamente da adipogênese e do metabolismo lipídico. A seleção de animais com teor e composição de gordura adequado para o consumidor é complexa pela difícil mensuração destas características, pela moderada herdabilidade e pelo desconhecimento dos genes envolvidos. Na última década, presenciamos um grande avanço na área da genômica bovina que resultou no sequenciamento completo do genoma e no desenvolvimento de chips de alta densidade de SNP. Este progresso científico, aliado aos avanços tecnológicos de equipamentos, resultou na identificação de genes responsáveis pela determinação de características quantitativas de interesse científico e comercial na bovinocultura. Este estudo teve como objetivo identificar e caracterizar genes associados à deposição e composição de gordura intramuscular em bovinos Nelore. Para este fim foi conduzido um estudo de associação genômica (Genome-wide association studies, GWAS) para identificar regiões genômicas associadas às características de interesse e identificar genes candidatos posicionais. Para o estudo de expressão diferencial foi conduzido um estudo do transcriptoma a partir do sequenciamento de RNA total (RNA-Seq) do músculo Longissimus dorsi. Foram utilizados 386 Nelores para a avaliação do teor de lipídeos total e perfil de ácidos graxos do músculo LD e, genotipagem com chip de alta densidade de SNP (Illumina SNP800 BeadChip). Um subconjunto de 14 animais, sendo sete animais de cada extremo para os valores genômicos estimados (GEBV) foi utilizado para o estudo de RNA-Seq. Foram encontradas 25 regiões genômicas (intervalos de 1 MB) associadas com deposição e composição de gordura intramuscular, as quais explicaram >= 1% da variância genética. Estas regiões foram identificadas nos cromossomos 2, 3, 6, 7, 8, 9, 10, 11, 12, 17, 26 e 27, muitas destas não foram previamente detectadas em outras raças. Nestas regiões foram identificados importantes genes e podem ajudar no entendimento da base genética envolvida na deposição e composição de gordura. As regiões genômicas e genes aqui identificados e apresentados contribuem para um melhor entendimento do controle genético da deposição e composição de gordura em gado de corte e ainda podem ser aplicados em programas de seleção genética de animais que produzam carne com qualidade e com perfil de gordura saudável ao homem.
Smajlovic, Dzenan. "Bestämning av FTO (Fat mass and obesity associated gene) polymorfism." Thesis, University of Kalmar, School of Pure and Applied Natural Sciences, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-804.
Повний текст джерелаVetenskapen har på senare år försökt fastställa de olika orsaker som leder till fetma. Det är känt att högt energiintag och för lite motion för eller senare hos de flesta individer resulterar i fetma. Det som kan konstateras är att ärftlighet i samspel med miljön vi lever i och påverkas av kan vara den huvudsakliga orsaken till en rad sjukdomar inklusive fetma. På senare år har forskare upptäckt olika gener som på ett eller annan sett är involverade i ämnesomsättningen. En sådan gen är ”fat mass and obesity associated gene”, FTO. Denna gen återfinns på kromosom 16 och har en storlek på 410 kilobaspar. Genen består av nio kodande områden, exoner, och 8 icke kodande områden, introner. Genens funktion är inte fastställd men den tycks både reglera ämnesomsättningen och lipolysen i kroppen. Tidigare studier har konstaterat att en specifik polymorfi i nukleotid rs9939609 medför ökad risk för sjuklig fetma. Uppsättningen som förekommer i nukleotiden uttrycks med A och T. Där dubbel uppsättning av A- allelen klassas som ärftlig risk för fetma. Syftet med detta examensprojekt är att bestämma polymorfi hos FTO genen med hjälp av två olika pyrosekvenserings- baserade metoder. Metod 1 bygger på extraktion av DNA från helblod, sedan amplifiering med PCR och slutligen pyrosekvensering. Metod 2, som jämfördes med metod 1, bygger på PCR direkt på helblod och pyrosekvensering. Blod från 97 friska individer analyserades. Med metod 1 konstaterades förekomst av följande genotyper i provmaterialet, 11 A/A homozygota, det vill säga har riskallelen för fetma i dubbeluppsättning, 50 A/T heterozygota och 36 T/T, vildtyp, som står för minskad ärftlig risk för fetma respektive ingen alls. Med metod 2 som skulle testas, visade sig resultatet överensstämma med metod 1. Med metod 2 erhölls följande resultat 11 A/A, 49 A/T och 34 T/T. Med metod 2 kunde inte 3 prov analyseras. Slutsatsen som kan dras utifrån studien i detta projekt är att metod 2 är likvärdig metod 1 ur analyssynpunkt. Metod 2 är arbetsbesparande tidsmässigt och även billigare då DNA extraktionssteget inte behöver genomföras.
2008:BL10
Science has for a long time looked for an answer for obesity. Obesity is often explained as the problem of the energy we eat and don’t use, but obesity might also have hereditary causes, where specific genes might play an important role. One of the recent genes found is the fat mass and obesity associated gene, FTO, which is located on chormosome 16 and has a size of 410 kilobasepairs. The gene is composed of nine exons and eight introns. The function of the gene is not known in detail, but studies has indicated that the gene could play a part in regulating the metabolism and fat cell lipolysis. The purpose with this examination degree project was to compare two methods for analysis of polymorphism in the FTO gene. Method 1 is based on DNA purification from whole blood, amplification with PCR, and finally detection using pyrosequencing. In method 2 PCR is performed on whole blood directly without prior DNA purification. Pyrosequencing was used with this method also to detect the polymorphism. Earlier studies have shown that theSNP (single nucleotid polymorphism) rs9939609, is associated with increased risk for obesity. Results obtained using method 1 were, 11 individuals had the A/A genotype, 50 was heterozygous (A/T), and 36 the wild type form (T/T), that is not associated with an increased risk for obesity. With method 2, the same result as with method 1 was obtained for the 94 samples of blood analyzed; 11 A/A, 49 A/T and 34 T/T were obtained. Remaining three samples of the 97 analyzed, failed in the pyrosequencing with method 2.
The conclusions with this degreeprojcet were that method 1 and 2 gave the same results. Method 2 is recommended as it is faster and less expensive, as no prior DNA purification is needed.
Lam, T. H. Jason. "Differential gene expression associated with phenotypic virulence of mycobacterium tuberculosis." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37769005.
Повний текст джерелаLam, T. H. Jason, and 林梓軒. "Differential gene expression associated with phenotypic virulence of mycobacterium tuberculosis." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37769005.
Повний текст джерелаEscaler, Margarita. "Changes in host gene expression associated with plant virus replication." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302215.
Повний текст джерелаMarbiah, M. "Identification of a gene regulatory network associated with prion replication." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1469415/.
Повний текст джерелаAravani, Dimitra. "Functional analysis of the coronary artery disease associated gene HHIPL1." Thesis, University of Leicester, 2017. http://hdl.handle.net/2381/39349.
Повний текст джерелаNdima, Tozama Beauty. "Gene expression associated with drought tolerance in Xerophyta viscosa Baker." Master's thesis, University of Cape Town, 2000. http://hdl.handle.net/11427/4309.
Повний текст джерелаHerophyta viscosa (Baker) is a monocytyledonous resurrection plant that can tolerate extremes of dessication. Upon rewatering, it rehydrates completely and assumes its full physiological activities. Studies on changes in gene expression associated with dehydration stress tolerance were conducted. A cDNA library constructed from m RNA isolated from dehydrated (85%, 37% and 5% relative water content) X. viscosa leaves, was differently screened. Of the 192 randomly selected cDNAs screened, 30 showed higher expression levels when X. viscosa was dehydrated while 20 showed lower expession. XVLEA, XVDH and XVLEC represent three cDNAs that were upregulated during dehydration stress. XVLEA showed the highest identity at the amino acid level with a late embryogenesis abundant protein, LEA29G, from Gossipium hirsutum (30%) and LEA D-29 from cotton (50%). XVDH exhibited significant identity to dehydrin proteins from Arabidopsis thaliana (45%) and Pisum sativum (43%) at the amino acid level. It encodes a glycine-rich protein (27kDa) which is largely hydrophilic and contains a hydrophobic segment at the C-terminus. XVLEC showed 28% identity and 50% similarity to a lectin-like protein from Arabidopsis thaliana. Southern blot analysis confirmed the presence of the three cDNAs in the X.viscosa genome. Both XVLEA and XVDH transcripts were highly expressed during dehydration- (37% RWC) and rehydration (4%, 32%, 72% RWC) treatment of the plant ͌ 1.0kb was observed. However, with XVDH a transcript of ͌ 1.0 kb and 1.09 kb were observed. XVDH transcripts accumulated in X. viscosa plants in response to low temperature, heat and dehydration stresses, as well as to exogenous supply of abscisic acid, ethylene and methyl jasmonate. Localization studies of the XVDH encoded protein showed that XVDH is located in the plasma membrane-cell wall region.
Asghar, Mobin. "Epstein-Barr virus associated diseases : immunotherapy and cytokine gene expression." Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/22388.
Повний текст джерелаWang, Qingliang. "QRX, a novel homeobox gene, is associated with retinal degeneration." Available to US Hopkins community, 2002. http://wwwlib.umi.com/dissertations/dlnow/3068223.
Повний текст джерелаRaskopp, Stina. "UNDERSTANDING MICROBE REGULATION OF THE PARKINSON DISEASE ASSOCIATED GENE LRRK2." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-237742.
Повний текст джерелаMikroflorans betydelse för människors hälsa och sjukdomar är ett framväxande och banbrytande forskningsfält. Forskning har inte bara visat på mikroflorans betydelse för friska tillstånd utan också för utveckling av sjukdomar, så som Parkinsons sjukdom (PD). PD är en neurodegenerativ sjukdom med symptom som innefattar rörelsestörningar; tremor, stelhet, bradykinesi och instabilitet. På molekylär nivå ses aggregerat alfa-synuclein inuti neuroner i hjärnan, i så kallade Lewy-kroppar samt förlust av dopaminerga neuroner i substantia nigra.Hypotesen som utformats i detta projekt utgick ifrån att mikroflorans sammansättning och interaktioner, medierar miljö- och livsstilsfaktorer vilket leder till utveckling av PD. För att testa hypotesen användes musmodellen C57BL / J6 i vildtyp form samt i transgen form. De transgena formerna bestod av två olika knock-in modeller; en som bär den vilda typen av humant Leucin-Rich-Repeat-Kinase 2 (hLRRK2) och en som bär den vanligaste kaukasiska mutationen av samma protein, G2019S. LRRK2 är ett tyrosin kinas som interagerar med Nucleotide-binding-oligomerization-domain-containing-protein 2 (NOD2), en cytosolisk mikrobpeptidreceptor. Analyser av LRRK2 och NOD2 utfördes på vildtypen av C57BL / J6-möss i specifikt patogenfria (SPF) förhållanden samt på möss som saknar exponering för levande mikrober, så kallade bakteriefria (GF). I de transgena mössen analyserades de genetiskt modifierade LRKK2-proteinerna, hLRRK2 och G2019S, samt NOD2 i möss i SPF förhållanden. Följande vävnader undersöktes; striatum, mellanhjärnan, hippocampus, tunntarmen och tjocktarmen med immunhistokemi (IHC) i kombination med Western blot-analys.Resultaten visade på en betydligt högre uttrycksnivå av LRRK2 i mikrobexponerade möss jämfört med GF möss med undantag för tjocktarmen där resultatet visade det motsatta. Dessutom visade resultaten en trend på lägre uttrycksnivåer av NOD2 i alla analyserade områden i hjärnan med undantag för striatum. För de transgena humana knock-in-LRKK2-proteinerna observerades ökat uttryck av LRKK2 i striatum och tjocktarm jämfört med G2019S, samt reducerat LRKK2 uttryck i mellanhjärnan. Resultaten visar på en stark korrelation mellan LRRK2-uttryck och tarmens mikroflora och implicerar förbättrad förståelse av mikroflorans roll i början och under progression av PD.
Herr, Taylor(Taylor J. ). "Dissecting the gene-regulatory circuitry of disease-associated genetic variants." Thesis, Massachusetts Institute of Technology, 2017. https://hdl.handle.net/1721.1/124573.
Повний текст джерелаThesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2019
Cataloged from student-submitted PDF version of thesis. "June 2019."
Includes bibliographical references (pages 89-91).
Disease-associated nucleotides lie primarily in non-coding regions, increasing the urgency of understanding how gene-regulatory circuitry impacts human disease. Here, we use the increasing availability of functional genomics datasets and models elucidating how regulatory proteins control genes, to evaluate the impact of genetic variants on the activity of diverse regulators. First, we generate a comprehensive compendium of predicted binding intensities across the entire genome for over 500 transcription factors. Second, we create a novel dataset to connect how these binding intensities change in the context of disease datasets. Third, we develop a statistical framework to integrate these two datasets using dimensionality reduction, latent cluster discovery, and topic modeling. We use these techniques to show that regulatory proteins with analogous biological functions share similar global changes in binding due to genome-wide genetic variation. We also use our framework to discover a latent set of topics behind all genomic locations in chromosome 1, to link the locations in each of the topic clusters with a class of related diseases, and to show that relevant biological processes are statistically enriched in the genomic locations most related to each cluster.
by Taylor Herr.
M. Eng.
M.Eng. Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science
Ye, Chaoyang. "Transcription regulation of adeno-associated viruses." Diss., Columbia, Mo. : University of Missouri-Columbia, 2007. http://hdl.handle.net/10355/4709.
Повний текст джерела"May 2007" The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. Includes bibliographical references.
Bendahmane, Abdelhafid. "Analysis of a gene-for-gene interaction associated with Rx-mediated resistance to potato virus X." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.389350.
Повний текст джерелаSeago, Julian. "Characterisation of ribonucleases and associated factors in Drosophila melanogaster." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343361.
Повний текст джерелаSen, Rwik. "REGULATION OF EUKARYOTIC TRANSCRIPTIONAL ELONGATION AND ASSOCIATED DNA REPAIR." OpenSIUC, 2016. https://opensiuc.lib.siu.edu/dissertations/1205.
Повний текст джерелаAmeen, Gazala. "Cloning and Characterization of rcs5, Spot Blotch Resistance Gene and Pathogen Induced Nec3 Gene Involved in Programmed Cell Death in Barley." Diss., North Dakota State University, 2019. https://hdl.handle.net/10365/29962.
Повний текст джерелаDauletbekov, Daniyar. "Adeno-associated virus mediated rhodopsin delivery in preventing secondary cone degeneration in rhodopsin knockout mice." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:041ef367-7ce6-467e-8988-b9735231bdf2.
Повний текст джерелаWärnmark, Anette. "Molecular mechanisms of gene activation by nuclear receptors and their associated coregulators /." Stockholm : [Karolinska Univ. Press], 2001. http://diss.kib.ki.se/2001/91-7349-032-6/.
Повний текст джерелаBockstael, Olivier. "Evaluation of gene transfer strategies using recombinant adeno-associated viruses for Parkinson's disease cell and gene therapy." Doctoral thesis, Universite Libre de Bruxelles, 2010. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210010.
Повний текст джерелаLes vecteurs dérivés des virus adéno-associés (rAAV) constituent des outils de choix pour le transfert de gènes dans les tissus cérébraux. Par ailleurs, de nombreuses applications nécessitent une régulation de l’expression du transgène. Nous disposons au laboratoire d’un vecteur rAAV inductible à la tétracycline (rAAV-TetON).
Nous décrivons dans ce travail :
i) le comportement du vecteur rAAV dérivé du sérotype 1 d’AAV utilisant la cassette d’expression TetON (rAAV2/1-TetON) comparé à celui du rAAV2/1 utilisant un promoteur constitutif pour l’expression du transgène (rAAV2/1-pCMV) dans le striatum et le mésencéphale (contenant la substance noire). A l’aide d’un vecteur rAAV2/1-TetON exprimant le GDNF, nous montrons que nous pouvons moduler le niveau d’expression du transgène dans le striatum par la dose d’inducteur administré aux animaux. Par ailleurs, nous montrons que le rAAV2/1-TetON présente dans le striatum une efficacité de transduction moindre que le rAAV2/1-pCMV mais qu’il présente un profil de biosécurité supérieur au rAAV2/1-pCMV car il limite fortement l’expression du transgène hors du striatum. De plus, le rAAV2/1-TetON n’entraîne pas de recrutement de lymphocytes T ni d’activation de la microglie dans le striatum. Lorsqu’il est injecté dans le mésencéphale, le vecteur rAAV2/1-TetON, contrairement au rAAV2/1-pCMV présente une expression préférentielle dans les neurones dopaminergiques de la SNpc et de l’aire tégmentale ventrale (VTA).
ii) le comportement des vecteurs rAAV2/1-pCMV et scAAV2/1-pCMV (vecteur « self-complémentaire » permettant une expression du transgène indépendamment de la synthèse du second brin du génome viral) dans la région neurogénique de la zone sous-ventriculaire (ZSV). Nous avons montré que les vecteurs rAAV2/1 infectent efficacement la ZSV et s’y expriment rapidement. Les vecteurs scAAV2/1 s’expriment plus rapidement dans la ZSV que les vecteurs rAAV2/1 (expression maximum à 24h et 48h, respectivement). De plus, les vecteurs rAAV2/1 présentent une efficacité de transfection importante pour les progéniteurs neuraux en prolifération (cellules C, transient amplifying progenitors) et les neuroblastes en migration (cellules A) mais pas pour les cellules souches neurales (cellules B). Nous observons, par ailleurs, que les rAAV2/1 induisent une baisse transitoire de la prolifération de la ZSV. Cet effet est indépendant de l’expression du génome et dépend donc probablement de la capside virale de nos vecteurs. De plus, cette baisse de prolifération n’induit pas d’apoptose. A long terme, nous observons des cellules exprimant le transgène dans la zone granulaire du bulbe olfactif, indiquant que la transduction des progéniteurs de la ZSV n’interfère pas avec leurs capacités de migration et de différenciation.
iii) l’efficacité de différents sérotypes de rAAV pour le transfert de gènes dans les cellules progénitrices neurales (NPC) in vitro. Nous avons montré que les rAAV peuvent transduire des NPC mais que l’efficacité spécifique des différents sérotypes testés varie en fonction de la région du cerveau fœtal et de l’espèce dont les NPC sont issues. Par ailleurs, les rAAV induisent une réduction drastique de la prolifération des cultures de NPC dépendante du sérotype de rAAV utilisé mais pas de l’origine fœtale des NPC ou de l’espèce dont elles sont issues.
Doctorat en Sciences biomédicales et pharmaceutiques
info:eu-repo/semantics/nonPublished
Kulis, Michael D. "Islet neogenesis associated protein-related protein from gene to folded protein /." Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-01112006-195113/.
Повний текст джерелаShuker, Suzanne, Committee Chair ; Doyle, Donald, Committee Member ; Orville, Allen, Committee Member ; Barry, Bridgette, Committee Member ; McCarty, Nael, Committee Member.
Kulis, Michael D. Jr. "Islet Neogenesis Associated Protein-Related Protein: From Gene to Folded Protein." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/10436.
Повний текст джерелаBorge, Kaja Sverdrup, Malin Melin, Patricio Rivera, Stein Istre Thoresen, Matthew Thomas Webster, Euler Henrik von, Kerstin Lindblad-Toh, and Frode Lingaas. "The ESR1 gene is associated with risk for canine mammary tumours." Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-200351.
Повний текст джерелаDaniels, Jan Peter. "Nuclear architecture and gene expression-associated protein families in trypanosoma brucei." Thesis, University of Oxford, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509914.
Повний текст джерелаHughes, Martin John Glenton. "A study of changes in gene expression associated with floral induction." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280454.
Повний текст джерелаGoodfellow, H. A. I. "Notch target gene regulation by chromatin associated factors and Ecdysone signalling." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599502.
Повний текст джерелаOkuchi, Yoshihisa. "Identification of Aging-Associated Gene Expression Signatures That Precede Intestinal Tumorigenesis." Kyoto University, 2016. http://hdl.handle.net/2433/217738.
Повний текст джерелаWang, Ling, Zhi Q. Yao, Jonathan P. Moorman, Yanji Xu, and Shunbin Ning. "Gene Expression Profiling Identifies IRF4-associated Molecular Signatures in Hematological Malignancies." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/6538.
Повний текст джерелаXu, Dan. "Cellular Immunity in Recombinant Adeno-Associated Virus Vector Mediated Gene Therapy." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1313504203.
Повний текст джерелаPachhain, Sudhan. "Analysis of gene expression associated with drug-induced hyperthermia in rat." Bowling Green State University / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1562330027757666.
Повний текст джерелаMiyamoto, Yoshihiro. "Endothelial Nitric Oxide Synthase Gene Is Positively Associated With Essential Hypertension." Kyoto University, 1999. http://hdl.handle.net/2433/182280.
Повний текст джерелаSabi, Essa. "Characterisation of ITGB3 and ITGA2B gene defects associated with Glanzmann thrombasthenia." Thesis, University of Sheffield, 2016. http://etheses.whiterose.ac.uk/13584/.
Повний текст джерелаReynolds, Paul Andrew. "Localization of a novel t(1;7) translocation associated with Wilms' tumour." Thesis, University of Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388159.
Повний текст джерелаLauramore, Amanda K. "Retinal cell tropism of adeno-associated viral (aav) vector serotypes." [Gainesville, Fla.] : University of Florida, 2004. http://purl.fcla.edu/fcla/etd/UFE0005301.
Повний текст джерелаTypescript. Title from title page of source document. Document formatted into pages; contains 71 pages. Includes Vita. Includes bibliographical references.
Pokiniewski, Katie Ann. "Understanding the cellular mechanism of Adeno-Associated Virus genome stabilization." Diss., Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/385837.
Повний текст джерелаPh.D.
The Adeno-Associated Virus(AAV) is a small, single stranded DNA virus that has been developed as a gene transfer vector. Early clinical trials using recombinant AAV vectors (rAAV) have identified the following concerns that need to be addressed in order to increase efficiency of these vectors. It has since been determined that AAV vector efficiency decreases due to the following mechanisms: ineffective endocytosis, endosomal degradation, inefficient trafficking, and the need to convert from a single-stranded AAV genome to a transcriptional active double-stranded form. The purpose of this study is to elucidate mechanisms that help stabilize the AAV genome in order to make it a more efficient vector for gene therapy. Previously, there have been studies using fluorescent labeling to track the movement of AAV into the nucleus. An integral part of AAV genomic stability may be the obstacles it encounters in the cytoplasm prior to entering the nucleus. Previous studies on improving AAV transduction have focused primarily on the nucleus. The study will hopefully shed light on the hurdles AAV encounters as it moves through the cytoplasm. Thus, this project has designed and utilized a new system for specifically tracking the status of AAV genomes in the cytoplasm as well as in the nucleus. This project utilizes a novel dual luciferase reporter system to track the movement of AAV particles from the cytoplasm into the nucleus to elucidate mechanisms that could contribute to the stabilization of the AAV genome. The novel dual reporter system is comprised of a single-stranded vector containing two different types of secreted luciferases: Cypridina Luciferase and Gaussia Luciferase. Cypridina Luciferase is placed under the control of a nuclear promoter and therefore it is expressed only in the nucleus. The second, Gaussia Luciferase, is under the control of a cytoplasmic promoter that will only be expressed in the cytoplasm upon the presence of T7 RNA polymerase. Using this dual reporter luciferase system along with RT qPCR quantification in a Hek293 cell line expressing the T7 RNA polymerase, demonstrated that genomes are present in the cytoplasm at 18 and 24 hours post infection. The second part of this study is using the dual reporter system to understand the trafficking patterns of rAAV and looking at ways to enhance transduction. One method could be administering rAAV vectors in conjunction with a drug; this approach may help overcome some of these cellular barriers encountered during infection as well as help stabilize the genome. Cidofovir (CDV) is a monophosphate nucleotide analogue that competitively inhibits the incorporation of deoxycytidine triphosphate into viral DNA via viral DNA polymerase. In vitro, CDV actively inhibits a number of DNA viruses including herpes viruses, adenovirus, polyomavirus, papillomavirus, and poxviruses. Cidofovir has already been approved by the Food and Drug Administration (FDA) for the treatment of cytomegalovirus (CMV) retinitis in patients with acquired immunodeficiency syndrome (AIDS). The effects of CDV on small DNA viruses that lack their own viral DNA polymerase, like AAV, has not been documented. Results have demonstrated that CDV is able to increase single-stranded rAAV transgene expression in the nucleus by 2 to 5 fold, depending on the cell type and concentration, in vitro, using both rAAV2 and rAAV8 luciferase reporter vectors. These results have been able to replicated using other reporter vectors: rAAV2-LacZ reporter, rAAV2-GFP, rAAV2-hAAT, and a rAAV8-GFP in vitro. Results have shown a dose-dependent increase in rAAV genomes with CDV pretreatment in HelaS3 cells via Southern Blot. Also southern blot analysis of cells pretreated with CDV then infected with rAAV revealed no difference in the amount of vector present between 0 and 2 hours post infection, suggesting CDV does not enhance viral entry but that CDV may enhance at steps downstream of viral entry. Using RNA sequencing and Ingenuity software analysis of HelaS3 cells pretreated with CDV, an increase in several genes of interest including those involved in the mechanism of viral exit were observed. These genes include Actin and vacuolar proteins, these molecules are involved in and associated with endosomal sorting complexes as well as required for transport inside the cell. This finding along with southern blot data supports the theory that CDV may enhance rAAV trafficking since we observed that CDV pretreatment enhances viral accumulation of rAAV vectors in both the cytoplasm and nucleus 24 hours post-infection. Also utilizing the dual luciferase reporter system an increase in transgene expression present with CDV pretreatment compared to PBS in both the cytoplasm and nucleus was observed suggesting that CDV may enhance with rAAV trafficking. These results taken together demonstrate that this dual reporter system is a powerful tool for understanding and improving rAAV trafficking. Also drugs like CDV can greatly contribute to the understanding of rAAV trafficking, and eventually lead to the development of novel strategies to increase overall efficiency of AAV transduction.
Temple University--Theses
Asimakopoulos, Fotios A. "Molecular analysis of chromosome 20q deletions associated with myeloproliferative disorders and myelodysplastic syndromes." Thesis, University of Cambridge, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388490.
Повний текст джерелаErickson, Sarah. "Using Unnatural Amino Acid Incorporation to Modify and Manipulate Adeno-Associated Virus:." Thesis, Boston College, 2020. http://hdl.handle.net/2345/bc-ir:108955.
Повний текст джерелаAdeno-Associated Virus (AAV) has been developed into a powerful therapeutic tool - in the last ten years it has acted as a gene-delivery vehicle in several approved therapeutics and many more therapeutics on trial. Despite extensive research, gaps in our understanding of AAV’s infectious cycle still exist, and further development is needed for the creation of improved gene therapy vectors. Technology to incorporate Unnatural Amino Acids (UAAs) into the AAV capsid has recently been developed, and could aid in both furthering our understanding of AAV’s biology and in the therapeutic advancement of AAV. In this work, we demonstrate how the functionalization of the AAV capsid using UAA incorporation can advance our control over the AAV capsid and aid in probing and manipulating AAV biology. We describe our use UAA incorporation to place a bio-orthogonal reactive handle into AAV’s capsid followed by functionalization with a targeting moiety and demonstrate the unprecedented amount of control that UAA incorporation provides in the creation of a functional virus conjugate. We are able to control both the precise placement and the stoichiometry of the targeting moiety on the AAV capsid, providing a platform that, for the first time, can undergo rigorous optimization analogous to that which medicinal chemists put small molecules through. We also describe the creation of a new platform to site-specifically modify the AAV capsid using cysteine incorporation, a technique that retains the ability to site-specifically modify the capsid as UAA incorporation does, but does not require the excess machinery that UAA incorporation requires. Next we discuss the incorporation of a photocaging amino acid, NBK, into the AAV capsid. Using NBK, we were able to effectively block AAV’s primary binding interaction with Heparan Sulfate Proteoglycan (HSPG) and control the timing of AAV infection using light to chemically remove the photo-protecting group. While photocaging the HSPG interaction is only a proof of concept, it demonstrates the remarkable amount of control that UAA incorporation affords, and lends insight to what could be accomplished using the functionalities that can be placed on the AAV capsid with UAAs
Thesis (PhD) — Boston College, 2020
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry
Krause, Lauren Kendall. "Gene Expression patterns in High-Altitude Pulmonary Edema: A Gene Microway Analysis." Yale University, 2008. http://ymtdl.med.yale.edu/theses/available/etd-08152007-111828/.
Повний текст джерела