Дисертації з теми "Antibody-Libraries"
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Charlton, Keith Alan. "Combinatorial antibody display libraries from sheep and their analysis." Thesis, University of Aberdeen, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.342711.
Повний текст джерелаBüssow, Konrad. "Arrayed cDNA libraries for antibody screening and systematic analysis of gene products." [S.l. : s.n.], 1998. http://www.diss.fu-berlin.de/1999/53/index.html.
Повний текст джерелаSkamaki, Kalliopi. "In vitro evolution of antibody affinity using libraries with insertions and deletions." Thesis, University of Cambridge, 2018. https://www.repository.cam.ac.uk/handle/1810/286439.
Повний текст джерелаSblattero, Daniele. "The Development of a Single Vector Recombination System to Make Large Phage Antibody Libraries." Doctoral thesis, SISSA, 1999. http://hdl.handle.net/20.500.11767/4385.
Повний текст джерелаPrendergast, D. "Discovery of tumour necrosis factor receptor-1 (p55) binding peptides using a phage display library." Thesis, Queen's University Belfast, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368468.
Повний текст джерелаPersic, Lidija. "The selection of specific phage antibodies from phage antibody libraries and their expression in different systems." Thesis, Open University, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299004.
Повний текст джерелаChiliza, Thamsanqa Emmanuel. "Antibody phage-displayed libraries derived from chicken immunoglobulin genes a source of highly specific diagnostic antibodies /." Diss., Pretoria : [s.n.], 2007. http://upetd.up.ac.za/thesis/available/etd-07012008-080736/.
Повний текст джерелаAlturki, Norah. "Expression of Biotinylated Multivalent Peptide Antigens in Bacteria for Rapid and Effective Generation of Single Domain Antibodies from Phage-displayed Antibody Libraries." Thesis, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23523.
Повний текст джерелаDiebolder, Philipp [Verfasser], and Roland E. [Akademischer Betreuer] Kontermann. "Generation of "LYmph Node Derived Antibody Libraries" (LYNDAL) : a concept for recovering human monoclonal antibodies with therapeutic potential / Philipp Diebolder ; Betreuer: Roland E. Kontermann." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2014. http://d-nb.info/112545069X/34.
Повний текст джерелаGiudice, Anna Maria <1991>. "ABC transporters of the A subfamily: potential prognostic markers and candidates for antibody selection from phage-display libraries for therapeutic use in Ewing sarcoma." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amsdottorato.unibo.it/9421/1/Giudice_AnnaMaria_tesi.pdf.
Повний текст джерелаZilkens, Kilian Johannes Carl Verfasser], Stefan [Akademischer Betreuer] [Dübel, and Andreas [Akademischer Betreuer] Gerstner. "Identification of novel tumor associated proteins in head and neck cancer using patient derived fresh tissue cDNA libraries and antibody repertoires / Kilian Johannes Carl Zilkens ; Stefan Dübel, Andreas Gerstner." Braunschweig : Technische Universität Braunschweig, 2020. http://d-nb.info/1217402411/34.
Повний текст джерелаDaugherty, Patrick Sean. "Screening combinatorial polypeptide libraries using bacterial surface display and fluorescence-activated cell sorting /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.
Повний текст джерелаRete, Cristian-Victor. "Libraries of dynamic peptides based on reversible native chemical ligation." Thesis, Strasbourg, 2018. http://www.theses.fr/2018STRAF032.
Повний текст джерелаThe possibility to use a new methodology for peptide bond exchange in aerobic biocompatible conditions has been investigated. We describe the assay optimization of dynamic combinatorial native chemical ligation (dynNCL) at the N-methyl-cysteine residue using model peptides. We further employ this optimized method for the screening of dynamic combinatorial peptide libraries in the presence and the absence of an antibody template. The effect that dynamic junction incorporation into non-antibody ligands has upon affinity was also studied. We propose that dynNCL can be used for the creation of a new class of designer sequences which can potentially provide the first example of artificial protein splicing
Cobaugh, Christian Wessel 1971. "Single scaffold antibody libraries created with high rates of mutagenesis or diversity focused for peptide recognition." 2007. http://hdl.handle.net/2152/15990.
Повний текст джерелаtext
Büssow, Konrad [Verfasser]. "Arrayed cDNA libraries for antibody screening and systematic analysis of gene products / Konrad Büssow." 1998. http://d-nb.info/960757937/34.
Повний текст джерелаShih, Yi-Ning, and 施憶寧. "The selection and characterization of Fab fragments for hepatitis C virus NS5A from phage display antibody libraries." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/19304418441007595951.
Повний текст джерела台北醫學院
細胞及分子生物研究所
90
Hepatitis C virus (HCV) is the major source of non-A, non-B hepatitis, which causes not only acute hepatitis by transient infection, but also chronic hepatitis, liver cirrhosis and hepatocellular carcinoma in human. Nonstructural protein 5 (NS5) of HCV possesses RNA polymerase activity and plays a key role in viral replication. In this study, we utilized two phage display antibody libraries to generate and analyze antibodies specific for NS5A. The sizes of antibody libraries containing kappa and lambda light chain are 1.3×107 and 2.1×106 pfu (plaque forming unit), respectively. Sequence analysis of 20 randomly selected clones indicated that these Fab fragments consisted of four groups, represented by NS5L6, NS5L13, NS5L14, and NS5K8. Of which, six NS5L6 clones contain identical heavy and light chain genes. Moreover, the light chain gene used by NS5L13 is almost identical to that of NS5L6 clones, suggesting that this light chain might be crucial for the NS5A-binding activity. The Fab expression of these chosen clones was verified as a 50 kDa protein on western blot using anti-human k or l light chain antibodies. ELISA results revealed that NS5L6, 13, and 14 all have NS5A-binding specificity comparable to that of sera from HCV-infected subjects. Viewed as a whole, our results suggested that the phage display antibody technology might provide an alternative way for the generation of human specific monoclonal antibodies. These generated antibodies could be useful for the development of therapeutic agents against infectious diseases in the future.
Penner, Gail. "Screening of 2 expression libraries with an antibody to a calcium binding ATPase inhibitor protein and sequencing of the isolated clones." 1989. http://hdl.handle.net/1993/16965.
Повний текст джерелаHuang, Yi-Jen, and 黃怡仁. "Engineering Anti-vascular Endothelial Growth Factor Single Chain Disulfide-stabilized Antibody Variable Fragments (sc-dsFv) with phage-displayed sc-dsFv Libraries." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/37609987889165955111.
Повний текст джерела國立陽明大學
微生物及免疫學研究所
98
Abstract: Phage-displayed fusion antibody on virus coat protein is a well established system which can efficiently engineer the antibody from genotype to phenotype to test the antibody physical properties on targeting to the antigen in vitro. The engineered antibody can be applicable in cancer therapy for the reason that antibody can carry effectors as cargos and aim to the specific cancer region in vivo. But in the reports from America Food and Drug Administration (FDA) approved antibody therapy in clinical trail, unknown antibody targeting will cause many unrelated, unexplained symptoms during antibody treatment. In order to reduce the side effects during antibody drug therapy, knowing the mechanism of protein-protein interaction by systematic analysis from experimental results in vitro are urgently needed to shorten time-consuming drug exploitation. Phage-displayed antibody targeting can be mimicked in vitro by panning amino acids library on antibody to a specific interested target. The recognition site on both antibody and antigen can also be analyzed the physicals between amino acid side-chain interactions in protein-protein interface from x-ray co-crystal structure. Thus, it can narrow down the design region for constructing the antibody library. In this study, antibody is modified from Bevacizumab (AvastinTM), which is one of the humanized anti-VEGF (vascular endothelial growth factor) monoclonal antibodies in the IgG form, and is the first approved by the FDA as a first-line treatment for metastatic colorectal cancer in combination with chemotherapy. This antibody-antigen interaction will be used as a model system to solve the problem on the antibody instability in the form of single chain fragment variable domains (scFv) which is shortcut and linked two domains from IgG and is also benefit with penetration in vivo during therapy. The purpose of this research is focus on building the a new phage display platform to enhance the antibody library selection with inter-domain disulfide bond between antibody variable domains to stabilize the scFv, to become the single chain disulfide-stabilized antibody variable fragments (sc-dsFv). In conclusion, the carboxyl terminal of the signal sequence manipulate the mass production of the sc-dsFv expressed on phage reveals a new fusion antibody platform on phage display system for further engineering designs, which is as convenient as the scFv-phage expression system but without the defect in the instability of the scFv. After the selection from phage-displayed sc-dsFv antibody libraries, the monoclonal antibody sc-dsFv can be further mass production with stability.
Pires, Tiago Maria Santos de Ochôa e. Azevedo. "Exploring Japonese quail immune repertoires for antibody discovery." Master's thesis, 2018. http://hdl.handle.net/10316/84753.
Повний текст джерелаAvian hosts are widely used for generation of antibodies and enable scalable and cost-effective production in eggs. Notably, hens also enable accelerating monoclonal antibody (mAb) discovery in biopharmaceutical industry since: 1) their phylogenetic distance from mammals ensures generation of robust and specific antibodies against conserved mammalian proteins and 2) the preparation of combinatorial phage-display libraries from chicken sources is simplified over mammalian systems. The present work has explored the potential of Japanese quail (Coturnix japonica) immune repertoires for mAb development. Multiple sequence search and analysis tools were used to revisit C. japonica genome (available since 2013) and identify/characterize genetic regions encoding for the light (LC) and heavy chain (HC) of quail immunoglobulin (Ig), namely using chicken data as reference.We were able to identify two main regions, corresponding to genes encoding quail Ig LC and HC. Simultaneously and supporting the genetic analysis, protein structural homology model predictions also allowed to identify folding conservation within certain domains, when comparing quail and chicken Igs. These supported the design of specific oligonucleotides that were subsequently used in PCR amplification of antibody fragments from a spleen cDNA samples, obtained from hyperimmune birds. Finally, preliminary NextGen sequencing (NGS) analysis of the fragments confirmed the expected distribution of conserved and variable regions.The present study is a relevant contribution for the implementation of methodologies that will allow generation of synthetic immunological repertoires from C. japonica hosts and subsequent development of therapeutic monoclonal antibodies.
A utilização de Aves como animal hospedeiro para geração de anticorpos é uma abordagem largamente explorada, que permite uma produção de anticorpos de baixo custo e escalável em ovos de aves poedeiras. Notavelmente, as galinhas também permitem acelerar a descoberta de anticorpos monoclonais (mAb) na indústria biofarmacêutica, uma vez que: 1) a sua distância filogenética em relação a mamíferos assegura a geração de anticorpos robustos e específicos contra proteínas conservadas de mamíferos e 2) a preparação de bibliotecas combinatórias de Phage-Display a partir de repertórios imunológicos de galinhas é simplificada em comparação com sistemas de mamíferos. O presente trabalho focou-se no estudo do potencial de repertórios imunológicos de codorniz japonesa (Coturnix japonica) no desenvolvimento de mAb. Foram utilizadas nomeadamente múltiplas ferramentas de busca e análise de sequências, para revisitar o genoma da C. japonica (disponível desde 2013) e identificar / caracterizar regiões genéticas que codificam a cadeia leve (LC) e pesada (HC) da imunoglobulina (Ig) de codorniz, usando dados de Galinha (Gallus gallus) como referência. Neste trabalho identificamos duas regiões análogas aos genes que codificam a a LC e HC da Ig de codorniz. Simultaneamente, e suportando a análise genética, previsões do modelo de homologia estrutural das proteínas codificadas por estes genes, permitiram identificar a conservação na estrutura dos domínios da Ig, entre a codorniz e a galinha. Estas análises permitiram o desenho de oligonucleótidos que foram subsequentemente utilizados na amplificação por PCR dos fragmentos de anticorpo, a partir de amostras de bibliotecas de cDNA do baço previamente obtidos de aves hiperimunes. Adicionalmente, uma análise preliminar por sequenciação NextGen (NGS) destes fragmentos confirmou a distribuição esperada das regiões conservadas e variáveis características de Ig.Este estudo representa uma contribuição relevante para a implementação de metodologias que permitem a geração de repertórios imunológicos utilizando a C. japonica e o posterior desenvolvimento de anticorpos monoclonais terapêuticos.
FCT
Petrul, Heike Maria. "Production of recombinant antibody molecules with specificity for proliferation antigens on human endothelium using phage display libraries for the optimization of the therapy of solid tumours and rheumatoid diseases = Herstellung von rekombinanten Antikörpermolekülen mit Spezifität für Proliferationsantigene auf humanen Endothelzellen basierend auf Phagen-Genbanken zur Optimierung der Therapie von soliden Tumoren und rheumatischen Erkrankungen /." 1999. http://www.gbv.de/dms/bs/toc/310687543.pdf.
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