Статті в журналах: "Antibacterial gla"

1

Gallagher, Louise, Alanna Smith, Kevin Kavanagh, Michael Devereux, John Colleran, Carmel Breslin, Karl G. Richards, Malachy McCann, and A. Denise Rooney. "Preparation and Antimicrobial Properties of Alginate and Serum Albumin/Glutaraldehyde Hydrogels Impregnated with Silver(I) Ions." Chemistry 3, no. 2 (June 14, 2021): 672–86. http://dx.doi.org/10.3390/chemistry3020047.

Повний текст джерела

Анотація:

Calcium alginate (CaALG) hydrogel beads and two sets of composite beads, formed from a combination of calcium alginate/propylene glycol alginate/human serum albumin (CaALG/PGA/HSA) and from calcium alginate with the quaternary ammonium salt, (3-(trimethoxysilyl)propyl)-octadecyldimethylammonium chloride (QA), (CaALG/QA), were prepared. Bovine serum albumin (BSA) was condensed with glutaraldehyde (GLA) to form a BSA/GLA hydrogel. The corresponding Ag+-containing gels of all of the above hydrogels were also formed, and slow leaching of the biocidal transition metal ion from the gels bestowed broad spectrum antimicrobial activity. In the absence of added Ag+, CaALG/QA was the only material to deliver marginal to moderate antibacterial and antifungal effects. The Ag+ impregnated hydrogel systems have the potential to maintain the antimicrobial properties of silver, minimising the risk of toxicity, and act as reservoirs to afford ongoing sterility.

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Baldwin, Susan L., Sasha E. Larsen, Valerie A. Reese, Tiffany Pecor, Brian Granger, Amit Khandhar, Christopher B. Fox, Steven G. Reed, and Rhea N. Coler. "Use of GLA-nanoalum as an effective adjuvant for a therapeutic ID93 TB vaccine." Journal of Immunology 200, no. 1_Supplement (May 1, 2018): 180.21. http://dx.doi.org/10.4049/jimmunol.200.supp.180.21.

Повний текст джерела

Анотація:

Abstract Tuberculosis (TB) caused by the intracellular bacterium Mycobacterium tuberculosis (Mtb) reportedly killed 1.3 million people in 2016 and is the leading cause of death caused by a single infectious organism. Increasingly worrisome is the ability of Mtb to develop extensive drug resistance. According to the 2017 WHO global TB report, there were 600,000 new rifampicin-resistant cases in 2016, and almost half a million cases with multiple drug resistant (MDR) TB. The development of new host-targeted therapeutic strategies that prevent the outgrowth of resistant mutants and/or modulate the immune response to combat Mtb infection and/or reduce disease pathology is one solution to prevent the generation of antibacterial resistance and treat drug resistant (DR)-TB. Here we evaluate a novel nanoalum adjuvant formulation containing a synthetic TLR4 agonist, glucopyranosyl lipid adjuvant (GLA), with our clinical ID93 protein as an immunotherapeutic vaccine. We show that immunotherapy with ID93+GLA-nanoalum is effective against Mtb when given as an adjunct to drug treatment in a mouse TB therapy model.

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Contini, Cristina, Simone Serrao, Barbara Manconi, Alessandra Olianas, Federica Iavarone, Giulia Guadalupi, Irene Messana, et al. "Characterization of Cystatin B Interactome in Saliva from Healthy Elderly and Alzheimer’s Disease Patients." Life 13, no. 3 (March 10, 2023): 748. http://dx.doi.org/10.3390/life13030748.

Повний текст джерела

Анотація:

Cystatin B is a small, multifunctional protein involved in the regulation of inflammation, innate immune response, and neuronal protection and found highly abundant in the brains of patients with Alzheimer’s disease (AD). Recently, our study demonstrated a significant association between the level of salivary cystatin B and AD. Since the protein is able to establish protein-protein interaction (PPI) in different contexts and aggregation-prone proteins and the PPI networks are relevant for AD pathogenesis, and due to the relevance of finding new AD markers in peripheral biofluids, we thought it was interesting to study the possible involvement of cystatin B in PPIs in saliva and to evaluate differences and similarities between AD and age-matched elderly healthy controls (HC). For this purpose, we applied a co-immunoprecipitation procedure and a bottom-up proteomics analysis to purify, identify, and quantify cystatin B interactors. Results demonstrated for the first time the existence of a salivary cystatin B-linked multi-protein complex composed by 82 interactors and largely expressed in the body. Interactors are involved in neutrophil activation, antimicrobial activity, modulation of the cytoskeleton and extra-cellular matrix (ECM), and glucose metabolism. Preliminary quantitative data showed significantly lower levels of triosophosphate isomerase 1 and higher levels of mucin 7, BPI, and matrix Gla protein in AD with respect to HC, suggesting implications associated with AD of altered glucose metabolism, antibacterial activities, and calcification-associated processes. Data are available via ProteomeXchange with identifiers PXD039286 and PXD030679.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Łazarski, Grzegorz. "Asperugo Procumbens: a Review of Botany, Traditional Uses, Phytochemistry and Pharmacology." Pakistan Journal of Medical and Health Sciences 15, no. 10 (October 30, 2021): 3092–95. http://dx.doi.org/10.53350/pjmhs2115103092.

Повний текст джерела

Анотація:

Asperugo procumbens is a medicinal plant widely distributed in the world, which is commonly used in the traditional medicine of the Middle East, while currently overlooked in European pharmacopoeias. The aim of the study is to present a comprehensive overview of A. procumbens in botany, traditional medicine, phytochemistry and pharmacology according to the up-to-date data. Scientific literature on the species was collected based on publicly available databases such as: PubMed, Web of Science, Scopus, Google, Google Scholar as well as monographs and PhD theses. In traditional medicine the aerial part of A. procumbens is used for treatment of skin infections, herpes, liver and respiratory tract diseases, to strengthen the heart and the nervous system, and others. Contemporary studies on the pharmacological activity of A. procumbens revealed that it exhibits a wide range of pharmacological properties, including antioxidant, antidepressant, sedative-hypnotic, antibacterial, as well as chemopreventive and antihypertensive properties. Despite many proven medicinal properties, the species does not have a developed phytochemical profile and its chemical composition is very poorly understood. The species is known to contain tannins, flavonoids and phenolics. Only the content of fatty acids is recognised in detail. The species is a good source of omega-3 fatty acids – stearidonic acid (SDA), α-linolenic acid (ALA), and omega-6 fatty acid – γ-linolenic acid (GLA). This article, based on the available literature, highlights the traditional uses, phytochemistry and pharmacological properties of A. procumbens, which may provide a foundation for further pharmacological study as well as clinical application of the species. Keywords: Asperugo procumbens, German madwort, Boraginaceae, traditional uses

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Falk, Shaun P., Andrew T. Ulijasz, and Bernard Weisblum. "Differential Assay for High-Throughput Screening of Antibacterial Compounds." Journal of Biomolecular Screening 12, no. 8 (December 2007): 1102–8. http://dx.doi.org/10.1177/1087057107308161.

Повний текст джерела

Анотація:

The previously described Bacillus subtilis reporter strain BAU-102 is capable of detecting cell wall synthesis inhibitors that act at all stages of the cell wall synthesis pathway. In addition, this strain is capable of detecting compounds with hydrophobic/ surfactant activity and alternative mechanisms of cell wall disruption. BAU-102 sequesters preformed β-gal in the periplasm, suggesting leakage of β-gal as the means by which this assay detects compound activities. A model is proposed according to which β-gal release by BAU-102 reflects activation of pathways leading to autolysis. The authors also report a simplified high-throughput assay using BAU-102 combined with the fluorogenic substrate N-methylumbelliferyl-β-D-galactoside as a single reagent. Cell wall inhibitors release β-gal consistently only after 60 min of incubation, whereas compounds with surfactant activity show an almost immediate release. A high-throughput screen of a 480-compound library of known bioactives yielded 8 compounds that cause β-gal release. These results validate the BAU-102 assay as an effective tool in antimicrobial drug discovery. ( Journal of Biomolecular Screening 2007:1102-1108)

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Zhao, Jing-Li, Meiping Zhang, and Hong-Li Zhou. "Microwave-Assisted Extraction, Purification, Partial Characterization, and Bioactivity of Polysaccharides from Panax ginseng." Molecules 24, no. 8 (April 23, 2019): 1605. http://dx.doi.org/10.3390/molecules24081605.

Повний текст джерела

Анотація:

Polysaccharides are a main active substance in Panax ginseng; however, microwave-assisted extraction used to prepare P. ginseng polysaccharides (MPPG) has rarely been reported, and knowledge of the bactericidal activity of P. ginseng polysaccharides remains low. Thus, this study was designed to investigate the extraction of P. ginseng polysaccharides by using two methods—hot water extraction and microwave-assisted extraction—and compare their chemical composition and structure. In addition, their antibacterial and antioxidant activities were also determined. The data implied that P. ginseng polysaccharides extracted by microwave-assisted extraction possessed a higher extraction yield than hot water extraction (WPPG) under optimized conditions, and the actual yields were 41.6% ± 0.09% and 28.5% ± 1.62%, respectively. Moreover, the preliminary characterization of polysaccharides was identified after purification. The WPPG with the molecular weight (Mw) of 2.07 × 105 Da was composed of Man, Rib, Rha, GalA, Glu, Gal, and Arab, and the typical characteristics of polysaccharides were determined by IR spectra. Compared with WPPG, MPPG had a higher Mw, uronic acid content, and Glu content. More importantly, the antioxidant activity of MPPG was higher than WPPG, which was probably ascribed to its highly Mw and abundant uronic acid content. Besides, both of them exhibited high bactericidal activity. These results demonstrate that microwave-assisted extraction is an effective method for obtaining P. ginseng polysaccharides, and MPPG could be applied as an antioxidant and antibacterial agent.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

He, Feng, Guang Yang, and Longjiang Yu. "Structural Investigation of an Antibacterial Polysaccharide from Streptomyces virginia H03." Zeitschrift für Naturforschung C 65, no. 5-6 (June 1, 2010): 317–21. http://dx.doi.org/10.1515/znc-2010-5-602.

Повний текст джерела

Анотація:

The chemical structure of a polysaccharide from the broth of cultured Streptomyces virginia H03 was investigated. There might be 1→2 and 1→4 and no 1→6 glycosidic linkages in the polysaccharide according to periodate oxidation and Smith degradation. Four fragments including 2,3,4,6-Me4-Man, 2,3,6-Me3-Gal, 2,4,6-Me3-Glc, and 3,6-Me2-Man were found in the methylated polysaccharide. Furthermore, the polysaccharide has a β-Glc(1→4)- α-Man(1→4)-α-Gal(1→3)-linked backbone and a branch at the C-2 position of (1→2)-linked mannose residues as determined by the method of nuclear magnetic resonance (NMR) spectroscopy. The assumed structure of the polysaccharide is [→3)-β-Glc(1→4)-α-Man (1→4)-α-Gal(1→]n. 2 ↑ α-Man(1 Additionally, the polysaccharide has a wide antibiogram and was found to be most effective against Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Pham, Quoc Thiet, Hieu Trung Nguyen, Sương Thi Thu Phan, Tram Thi Quynh Doan, and Ky Minh Nguyen. "STUDY OF BIOLOGICAL ACTIVITIES OF SLENDER AMARANTH (Amaranthus lividus L.) IN GIA LAI PROVINCE." Scientific Journal of Tra Vinh University 1, no. 36 (April 8, 2020): 64–70. http://dx.doi.org/10.35382/18594816.1.36.2019.317.

Повний текст джерела

Анотація:

Slender amaranth (Amaranthus lividus L.) is a popular plant in Gia Lai Province, with anti-cancer, antioxidant and antibacterial properties. The study presents the experimental results of biological activity of Amaranthus lividus L. collected in Gia Lai Province. Evaluation of anti-tumor activity (IC50) showed good results with EtOAc and n–hexane with extracts of 8.0 µg/ml and 11.2 µg/ml (≤ 20 µg/ml, NCI) respectively. The comparison results showed that the EtOAc extract from Amaranthus lividus L. has better antibacterial activity than the MeOH extract. However, the investigated extracts from Amaranthus lividus L. were incapable of inhibiting oxidation at low concentrations of 1-64 µg/ml. Results of biologically active ingredients in Amaranthus lividus L. illustratedthe applied prospects of natural medicine sources.

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Falk, Shaun P., James W. Noah, and Bernard Weisblum. "Screen for Inducers of Autolysis in Bacillus subtilis." Antimicrobial Agents and Chemotherapy 54, no. 9 (July 6, 2010): 3723–29. http://dx.doi.org/10.1128/aac.01597-09.

Повний текст джерела

Анотація:

ABSTRACT We describe a primary high-throughput screen that uses the reporter strain Bacillus subtilis BAU-102 to identify antibiotics that induce autolysis. The screen measures autolysis in terms of the incipient release of recombinant Escherichia coli β-galactosidase (β-Gal) from the periplasmic space of B. subtilis owing to a loss of integrity of the cell wall. In a model screen, β-Gal release values for 79 members of a library consisting of antibiotics and related compounds were collected, sorted, and plotted as a function of rank. Inducers of autolysis, which included compounds that inhibit cell wall synthesis and those that do not, were readily differentiated from other members of the library on the basis of their elevated β-galactosidase release responses. The results of the BAU-102 model screen called attention to the antibacterial activity of drugs normally used in other applications, describable as “repurposed.” Thus, the screen independently identified the potential antibacterial properties of the antifungal drug miconazole and of the antileishmaniasis drug miltefosine. Daptomycin-induced release of β-Gal was also detected and occurred in a Ca2+-dependent manner.

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Ito, Yoshiyuki, Yasushi Kawai, Kensuke Arakawa, Yoshiko Honme, Takashi Sasaki, and Tadao Saito. "Conjugative Plasmid from Lactobacillus gasseri LA39 That Carries Genes for Production of and Immunity to the Circular Bacteriocin Gassericin A." Applied and Environmental Microbiology 75, no. 19 (August 7, 2009): 6340–51. http://dx.doi.org/10.1128/aem.00195-09.

Повний текст джерела

Анотація:

ABSTRACT Gassericin A is a circular bacteriocin produced by Lactobacillus gasseri strain LA39. We found a 33,333-bp plasmid, designated pLgLA39, in this strain. pLgLA39 contained 44 open reading frames, including seven genes related to gassericin A production/immunity (gaa), as well as genes for replication, plasmid maintenance, and conjugative transfer. pLgLA39 was transferred from LA39 to the type strain of L. gasseri (JCM 1131) by filter mating. The transconjugant exhibited >30-fold-higher more resistance to gassericin A and produced antibacterial activity. Lactobacillus reuteri LA6, the producer of reutericin 6, was proved to harbor a plasmid indistinguishable from pLgLA39 and carrying seven genes 100% identical to gaa. This suggests that pLgLA39 might have been transferred naturally between L. gasseri LA39 and L. reuteri LA6. The seven gaa genes of pLgLA39 were cloned into a plasmid vector to construct pGAA. JCM 1131T transformed with pGAA expressed antibacterial activity and resistance to gassericin A. pGAA was segregationally more stable than a pGAA derivative plasmid from which gaaA was deleted and even was more stable than the vector. This suggests the occurrence of postsegregational host killing by the gaa genes. pLgLA39 carried a pemIK homolog, and segregational stabilization of a plasmid by the pLgLA39-type pemIK genes was also confirmed. Thus, pLgLA39 was proved to carry the genes for at least two plasmid maintenance mechanisms, i.e., gaa and pemIK. Plasmids containing a repA gene similar to pLgLA39 repA were distributed in several L. gasseri strains.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Xia, Yulei, та Qiwei Zhai. "IL-1β enhances the antibacterial activity of astrocytes by activation of NF-κB". Glia 58, № 2 (11 серпня 2009): 244–52. http://dx.doi.org/10.1002/glia.20921.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Li, Mo, Henan Zhang, Xinyu Hu, Yumeng Liu, Yanfeng Liu, Meijun Song, Rina Wu, and Junrui Wu. "Isolation of a New Polysaccharide from Dandelion Leaves and Evaluation of Its Antioxidant, Antibacterial, and Anticancer Activities." Molecules 27, no. 21 (November 7, 2022): 7641. http://dx.doi.org/10.3390/molecules27217641.

Повний текст джерела

Анотація:

Dandelion, in China, has a long history as a medicinal and edible plant, and possesses high nutritional and medical value. The present study aimed to isolate a new polysaccharide (DLP-3) from dandelion leaves and to evaluate its antioxidant, antibacterial, and anticancer activities. The structure of DLP-3 was analyzed using HPLC, FT-IR, SEM, GC-MS, and NMR spectroscopy. DLP-3 mainly consisted of Man, Rha, GlcA, Glc, Gal, and Ara with molar ratios of 2.32, 0.87, 1.21, 3.84, 1.00, and 1.05, respectively, with a molecular weight of 43.2 kDa. The main linkages of DLP-3 contained (1→4)-α-d-Glc, (1→4,6)-α-d-Glc, (1→6)-α-d-Gal, (1→2)-α-d-Man, (1→4)-α-d-Man, β-l-Ara-(1→, and α-l-Rha-(1→. DLP-3 exhibited a smooth surface, purely flake-like structure, and a triple helix conformation. Moreover, DLP-3 presented obvious antioxidant and antibacterial activities in a concentration-dependent manner. DLP-3 showed significant anticancer activities by inhibiting tumor cell proliferation. These findings provide a theoretical basis for the application of DLP-3 as a natural functional active substance in functional foods.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Sulistyanti, Aprillia Dini, Emma Kamelia, Hadiyat Miko, Tritania Ambarwati, and Rena Setiana. "MENGUNYAH BUAH APEL ROYAL GALA TERHADAP PEMBENTUKAN PLAK DAN DERAJAT KEASAMAN SALIVA PADA SISWA KELAS VI SDIT ASSUNNAH KOTA CIREBON." JDHT Journal of Dental Hygiene and Therapy 2, no. 1 (April 13, 2021): 27–33. http://dx.doi.org/10.36082/jdht.v2i1.214.

Повний текст джерела

Анотація:

Plaque and pH saliva are factors that can cause dental caries. The prevalence of caries according to RISKESDAS (2018) reached 45.3% in Indonesia. WHO (2012) states that the prevalence of caries for school children at 60%-90%. They are fond of consuming cariogenic foods that can increase the risk of caries. The Royal Gala Apple contains antibacterial that can inhibit plaque formation and have a slight acid flavor that can stimulate saliva flow. Aim: To know the influence of chewing the Royal Gala Apples to plaque formation and pH saliva in grade VI students SDIT Assunnah Cirebon. Method: Quasi experiment with one group pretests posttest design. Samples of 33 students. The samples were instructed to chew the Royal Gala Apple 100 gr for 5 minutes. Plaque measured by PHP index and pH saliva is measured with universal indicator paper. Results: The normality test of Kolmogorov-Smirnov is obtained by the plaque and pH saliva results before and after chewing the royal Gala p-value of < 0.05 so, that distribution data is abnormal. Data analyzed using Wilcoxon test was obtained PHP plaque index and pH saliva before and after chewing Apple Royal Gala p-value: 0.000 (sig < 0.05) Conclusion: there is a chewing effect of the Royal Gala Apple Fruit against plaque formation and pH saliva in grade VI students SDIT Assunnah Cirebon.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Ha, Thi Quyen, and Thi Minh Tu Hoa. "Selection of lactic acid bacteria producing bacteriocin." Journal of Vietnamese Environment 8, no. 5 (January 17, 2017): 271–76. http://dx.doi.org/10.13141/jve.vol8.no5.pp271-276.

Повний текст джерела

Анотація:

Lactic acid bacteria were isolated from 10 samples of the traditionally fermented foods (5 samples of Vietnamese fermented pork roll and 5 samples of the salted field cabbage) and 5 samples of fresh cow milks collected from households in Vietnam. 22 strains of lactic acid bacteria were isolated for inhibition to Lactobacillus plantarum JCM 1149. Of these, only 2 strains including DC1.8 and NC1.2 have rod shape, the others have coccus shape. 7 strains showing higher antibacterial activity were selected for checking spectrum of antibacteria with indicator bacteria consistting of Bacillus subtilis ATCC 6633, Enterococcus faecium JCM 5804 and Staphylococcus aureus TLU. By which, 3 strains including NC3.5 (from Vietnamese fermented pork roll), DC1.8 (from salted field cabbage) and MC3.19 (from fresh cow milk) were selected because of their higher antibacterial ability. However, the antibacterial activity of the lactic acid bacteria can be based on their disposable compounds and some other antibacterial compounds produced during their growth (such as lactic acid, H2O2, bacteriocins, etc.). For seeking lactic acid bacteria with capability of producing bacteriocins, antibacterial compounds with protein nature, 3 above strains were checked sensitiveness to proteases (including protease K, papain, α – chymotrypsin and trypsin). Because bacteriocins are proteinaceous antibacterial compounds, so their antibacterial activity will be reduced if proteases are added. The result showed DC1.8 and MC3.19 were capable of producing bacteriocin during culture process. They were identified as Lactobacillus acidophilus and Lactococcus lactis and classified, respectively, based on analysis chemical characterisitcs by standard API 50 CHL kit and phylogeny relationship by 16s rRNA sequences. Các chủng vi khuẩn lactic được phân lập từ 10 mẫu thực phẩm lên men truyền thống (5 mẫu nem chua, 5 mẫu dưa cải bẹ muối) và 5 mẫu sữa bò tươi được thu thập từ các hộ gia đình ở Việt Nam. 22 chủng vi khuẩn lactic đã được phân lập với tiêu chí có khả năng kháng lại vi khuẩn kiểm định Lactobacillus plantarum JCM 1149. Trong số đó, 2 chủng DC1.8 và NC1.2 có tế bào hình que, các chủng còn lại có tế bào hình cầu. 7 chủng thể hiện hoạt tính kháng khuẩn cao được lựa chọn để xác định phổ kháng khuẩn rộng hơn với ba loài vi khuẩn kiểm định Bacillus subtilis ATCC 6633, Enterococcus faecium JCM 5804 và Staphylococcus aureus TLU. Từ đó lựa chọn được 3 chủng có hoạt tính kháng khuẩn cao hơn hẳn. Các chủng này gồm NC3.5 phân lập từ nem chua, DC1.8 phân lập từ dưa cải bẹ muối và MC3.19 phân lập từ sữa bò tươi. Tuy nhiên, hoạt tính kháng khuẩn của vi khuẩn lactic bao gồm những hợp chất nội tại có trong nó và cả những hợp chất được sinh ra trong quá trình phát triển của nó (như axit lactic, H2O2, bacteriocin, …). Với định hướng tìm chủng vi khuẩn lactic có khả năng sinh bacteriocin, chất kháng khuẩn có bản chất protein, 3 chủng trên được kiểm tra độ nhạy cảm với các protease (gồm protease K, papain, α – chymotrypsin và trypsin). Do bacteriocin là chất kháng khuẩn có bản chất protein nên hoạt tính kháng khuẩn của chúng sẽ bị giảm nếu protease được bổ xung vào. Kết quả lựa chọn được chủng DC1.8 và MC3.19 có khả năng sinh bacteriocin. Hai chủng này được phân loại đến loài nhờ vào phân tích đặc điểm sinh hóa bằng kit API 50 CHL và mối quan hệ di truyền thông qua trình tự gen 16s rRNA. Kết quả phân loại đã xác định chủng DC1.8 thuộc loài Lactobacillus acidophilus và chủng MC3.19 thuộc loài Lactococcus lactis.

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Unalan, Irem, Stefan J. Endlein, Benedikt Slavik, Andrea Buettner, Wolfgang H. Goldmann, Rainer Detsch та Aldo R. Boccaccini. "Evaluation of Electrospun Poly(ε-Caprolactone)/Gelatin Nanofiber Mats Containing Clove Essential Oil for Antibacterial Wound Dressing". Pharmaceutics 11, № 11 (1 листопада 2019): 570. http://dx.doi.org/10.3390/pharmaceutics11110570.

Повний текст джерела

Анотація:

The objective of this study was to produce antibacterial poly(ε-caprolactone) (PCL)-gelatin (GEL) electrospun nanofiber mats containing clove essential oil (CLV) using glacial acetic acid (GAA) as a “benign” (non-toxic) solvent. The addition of CLV increased the fiber diameter from 241 ± 96 to 305 ± 82 nm. Aside from this, the wettability of PCL-GEL nanofiber mats was increased by the addition of CLV. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of CLV, and the actual content of CLV was determined by gas chromatography–mass spectrometry (GC-MS). Our investigations showed that CLV-loaded PCL-GEL nanofiber mats did not have cytotoxic effects on normal human dermal fibroblast (NHDF) cells. On the other hand, the fibers exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli. Consequently, PCL-GEL/CLV nanofiber mats are potential candidates for antibiotic-free wound healing applications.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Sherwani1, Neelam, Raeid M. M. Abed, Sergey Dobretsov, and Sheji Mary. "Antibacterial and Antifungal Activities of Cyanobacterial Strains Isolated from Hot Springs in Oman." Sultan Qaboos University Journal for Science [SQUJS] 20, no. 1 (June 1, 2015): 11. http://dx.doi.org/10.24200/squjs.vol20iss1pp11-19.

Повний текст джерела

Анотація:

In this study, cyanobacterial microbial mats from five hot springs in Oman, namely Al Kasfah Rustaq, Al Thwara Nakhl, Al–Ali Hammam, Gala and Bowsher, were characterized using direct microscopy. Nine monoclonal cyanobacterial cultures were obtained and their extracts in butanol, dichloromethane (DCM) and hexane were screened for antibacterial and antifungal activities. Direct microscopy revealed the presence of 12 different unicellular and filamentous morphotypes, with different distribution in the various mats. Temperature seems to be one of the most important parameters that accounts for the differences in cyanobacterial composition of the mats. Cells of the nine isolates and their aqueous supernatants were subsequently extracted with butanol, DCM and hexane. Dried extracts were tested against nine bacterial (i.e. gram +ve Staphylococcus aureus, Bacillus subtilis and gram –ve, Escherichia coli, Klebsiella pneumoniae, Salmonella choleraesuis, S. enterica, Psuedomonas aeruginosa, Providencia stuartii, and Acinetobacter calcoaceticus) and two fungal pathogens (Rhizoctonia solani and Pythium sp.). All isolates exhibited antibacterial and antifungal activities, which depended mainly on the type of cyanobacterial culture, type of solvent used and the pathogen tested. The highest antibacterial activity was observed in Phormidium species, and butanol was found to be the most appropriate solvent to extract bioactivity from these cyanobacterial species. The results of this study suggest that thermal springs in Oman harbor diverse types of cyanobacteria, which may constitute an important source of antibacterial and antifungal compounds. Further investigation is needed to purify these compounds and find their chemical compositions and modes of action.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Kim, Jin-Seong, Jae-Bum Park, Seung-Won Jang, Deok-Ho Kwon, Mi-Hee Jang, Mi-Ok Lee, and Suk-Jin Ha. "Determination of Antibacterial Activity from Tricholoma matsutake Extract and Its Application to Low Salted Jeot-gal." KSBB Journal 30, no. 5 (October 27, 2015): 253–56. http://dx.doi.org/10.7841/ksbbj.2015.30.5.253.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Guyot, Nicolas, Hervé Meudal, Sascha Trapp, Sophie Iochmann, Anne Silvestre, Guillaume Jousset, Valérie Labas та ін. "Structure, function, and evolution ofGga-AvBD11, the archetype of the structural avian-double-β-defensin family". Proceedings of the National Academy of Sciences 117, № 1 (23 грудня 2019): 337–45. http://dx.doi.org/10.1073/pnas.1912941117.

Повний текст джерела

Анотація:

Out of the 14 avian β-defensins identified in theGallus gallusgenome, only 3 are present in the chicken egg, including the egg-specific avian β-defensin 11 (Gga-AvBD11). Given its specific localization and its established antibacterial activity,Gga-AvBD11 appears to play a protective role in embryonic development.Gga-AvBD11 is an atypical double-sized defensin, predicted to possess 2 motifs related to β-defensins and 6 disulfide bridges. The 3-dimensional NMR structure of the purifiedGga-AvBD11 is a compact fold composed of 2 packed β-defensin domains. This fold is the archetype of a structural family, dubbed herein as avian-double-β-defensins (Av-DBD). We speculate thatAvBD11emanated from a monodomain gene ancestor and that similar events might have occurred in arthropods, leading to another structural family of less compact DBDs. We show thatGga-AvBD11 displays antimicrobial activities against gram-positive and gram-negative bacterial pathogens, the avian protozoanEimeria tenella, and avian influenza virus.Gga-AvBD11 also shows cytotoxic and antiinvasive activities, suggesting that it may not only be involved in innate protection of the chicken embryo, but also in the (re)modeling of embryonic tissues. Finally, the contribution of either of the 2Gga-AvBD11 domains to these biological activities was assessed, using chemically synthesized peptides. Our results point to a critical importance of the cationic N-terminal domain in mediating antibacterial, antiparasitic, and antiinvasive activities, with the C-terminal domain potentiating the 2 latter activities. Strikingly, antiviral activity in infected chicken cells, accompanied by marked cytotoxicity, requires the full-length protein.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Ousaaid, Driss, Hassan Laaroussi, Meryem Bakour, Hayat Ennaji, Badiaa Lyoussi, and Ilham El Arabi. "Antifungal and Antibacterial Activities of Apple Vinegar of Different Cultivars." International Journal of Microbiology 2021 (August 7, 2021): 1–6. http://dx.doi.org/10.1155/2021/6087671.

Повний текст джерела

Анотація:

This study was designed to assess the antimicrobial potencies of apple vinegar against pathogenic microbes. The acidity and total phenolic content were carried out by titration with NaOH 0.1 N and the Folin–Ciocalteu method, respectively, while the spread plate method, agar well diffusion, and MIC assays were used to determine the antimicrobial activities of different vinegar samples. Acidity and phenolic content were dependent on the variety, where the highest values were observed in S2 with 4.02 ± 0.04% and 1.98 ± 0.05 mg GAE/mL for acidity and total phenolic content, respectively. The spread plate method revealed that samples S1 and S2 obtained from the Red delicious variety and Golden delicious variety, respectively, inhibit the growth of all tested strains, while S3 obtained from different varieties and S4 obtained from the Gala royal variety inhibit only two microbes (Escherichia coli and Vibrio cholerae). Sample S1 presented moderate antimicrobial effect against all examined strains with a diameter of inhibition ranging from 11 ± 0.7 to 19 ± 0.5 mm and with MIC values ranging between 1/2 and 1/100. The findings of the current study confirm the usefulness of apple vinegar as a natural sanitizer that inhibits the growth of pathogenic microbes.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Seweryn, Ewa, Emilia Królewicz, Kamilla Stach, and Irena Kustrzeba-Wójcicka. "Nutritional and allergenic properties of hen eggs." Postępy Higieny i Medycyny Doświadczalnej 72 (April 6, 2018): 205–14. http://dx.doi.org/10.5604/01.3001.0011.7339.

Повний текст джерела

Анотація:

Chicken eggs, along with cow milk, are the most important source of proteins and other valuable nutrients that are introduced to a baby`s diet. Certain components of eggs, besides nutritional, also have other biological functions. Both proteins, phospholipids or carotenoids, are bioactive components which affect the physiological processes in the human body. Regular consumption of chicken eggs rich in substances with antibacterial, anti-inflammatory, immunomodulatory and antioxidant properties may contribute to reducing the incidence of certain lifestyle diseases. Ovomucoid, as a glycoprotein which inhibits bacterial protease, is a component of eggs with bactericidal properties. Similarly, the ovotransferrin protein has a bacteriostatic effect on the Escherichia coli strain or Streptococcus mutans. Due to the strong antioxidant properties, phospholipids, vitamin E and folic acid are extremely valuable egg components. It is believed that the high antioxidant potential of these compounds is important in preventing the development of atherosclerosis and other metabolic syndromes. It is also worth mentioning lutein and zeaxanthin, which are dyes that form a protective barrier against the degeneration of the macula of the human eye. An extremely important function for the human immune system is also met by lysozyme, which stimulates the synthesis of interferon, stimulating the immune response. Unfortunately, hypersensitivity to chicken eggs is one of the most common food allergies in children and affects 0.5-9% of the population. The major egg allergens (Gallus spp.): ovomucoid (Gal d 1), ovalbumin (Gal d 2), conalbumin (Gal d 3) and lysozyme (Gal d 4) are present in egg white and most often cause allergic reactions in children. Minor allergens: serum albumin (Gal d 5) and YGP42 protein (Gal d 6) are found in the egg yolk and are more likely to sensitize adults.

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Xie, Zhipeng, Hanqi Wei, Jiahui Meng, Tong Cheng, Yanting Song, Manchuriga Wang, and Yingxia Zhang. "The Analogs of Temporin-GHa Exhibit a Broader Spectrum of Antimicrobial Activity and a Stronger Antibiofilm Potential against Staphylococcus aureus." Molecules 24, no. 22 (November 18, 2019): 4173. http://dx.doi.org/10.3390/molecules24224173.

Повний текст джерела

Анотація:

The abuse of antibiotics has led to the emergence of multidrug-resistant bacteria, which is becoming a serious worldwide problem people have to face. In our previous study, temporin-GHa (GHa) cloned from Hylarana guentheri showed antimicrobial activity against Gram-positive bacteria. In order to improve its therapeutic potential, we used a template-based and a database-assisted design to obtain three derived peptides by replacing the histidine at both ends of GHa with lysine, which exhibited faster and stronger bactericidal activity and a broader spectrum than the parent peptide. GHaK and GHa4K targeted to the bacterial membrane to exert their antibacterial activities at a faster membrane damage rate. The derived peptides inhibited the initial adhesion and the formation of Staphylococcus aureus biofilms, and eradicated the mature biofilms, which indicated that the derived peptides effectively penetrated the biofilm and killed bacteria. The therapeutic index (TI) and cell selectivity index (CSI) of the derived peptides increased significantly, which means a broader therapeutic window of the derived peptides. The derived peptides with improved activity and cell selectivity have the potential to be the promising candidates for the treatment of S. aureus infections. Our research also provides new insights into the design and development of antimicrobial peptides.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Kara, Mohammed, Amine Assouguem, Omkulthom Mohamed Al kamaly, Safaâ Benmessaoud, Hamada Imtara, Hamza Mechchate, Christophe Hano, Abdou Rachid Zerhouni, and Jamila Bahhou. "The Impact of Apple Variety and the Production Methods on the Antibacterial Activity of Vinegar Samples." Molecules 26, no. 18 (September 7, 2021): 5437. http://dx.doi.org/10.3390/molecules26185437.

Повний текст джерела

Анотація:

Apple vinegar is a natural product widely used in food and traditional medicine as it contains many bioactive compounds. The apple variety and production methods are two factors that play a major role in determining the quality of vinegar. Therefore, this study aims to determine the quality of apple vinegar samples from different varieties (Red Delicious, Gala, Golden Delicious, and Starking Delicious) prepared by three methods using small apple pieces, apple juice, and crushed apple, through determining the physicochemical properties and antibacterial activity of these samples. The antibacterial activity was studied against five pathogenic bacteria: Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli (ATB: 57), Escherichia coli (ATB: 97), and Pseudomonas aeruginosa, using two methods, disk diffusion and microdilution, for determining the minimum inhibitory concentrations and the minimum bactericidal concentrations. The results of this study showed that the lowest pH value was 3.6 for Stark Delicious, obtained by liquid fermentation, and the highest acetic acid values were 4.7 and 4% for the vinegar of Red Delicious and Golden Delicious, prepared by solid fermentation, respectively. The results of the antibacterial activity showed considerable activity of apple vinegar on the tested strains. Generally, the Staphylococcus aureus strain appears less sensitive and Pseudomonas aeruginosa seems to be very sensitive against all samples, while the other strains have distinct sensitivities depending on the variety studied and the method used. A higher antibacterial activity was found in vinegar obtained by the apple pieces method and the Red Delicious variety, with a low MIC and MBC recorded, at 1.95 and 3.90 µL/mL, respectively. This study has shown that the choice of both apple variety and production method is therefore an essential step in determining and aiming for the desired quality of apple vinegar.

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Na, Choon-Ki, Ga-Yeon Park, and Jee-June Song. "Preparation of Filtration Material with Adsorption/Antibacterial Property by Photografting GMA and Subsequent Chemical Modification and Its Performance Evaluation." Journal of the Korean Society for Environmental Technology 20, no. 3 (June 30, 2019): 178–86. http://dx.doi.org/10.26511/jkset.20.3.5.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Chikalovets, Irina, Alina Filshtein, Valentina Molchanova, Tatyana Mizgina, Pavel Lukyanov, Olga Nedashkovskaya, Kuo-Feng Hua, and Oleg Chernikov. "Activity Dependence of a Novel Lectin Family on Structure and Carbohydrate-Binding Properties." Molecules 25, no. 1 (December 30, 2019): 150. http://dx.doi.org/10.3390/molecules25010150.

Повний текст джерела

Анотація:

A GalNAc/Gal-specific lectins named CGL and MTL were isolated and characterized from the edible mussels Crenomytilus grayanus and Mytilus trossulus. Amino acid sequence analysis of these lectins showed that they, together with another lectin MytiLec-1, formed a novel lectin family, adopting β-trefoil fold. In this mini review we discuss the structure, oligomerization, and carbohydrate-binding properties of a novel lectin family. We describe also the antibacterial, antifungal, and antiproliferative activities of these lectins and report about dependence of activities on molecular properties. Summarizing, CGL, MTL, and MytiLec-1 could be involved in the immunity in mollusks and may become a basis for the elaboration of new diagnostic tools or treatments for a variety of cancers.

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Amine, Daoudi, Bammou Mohamed, Ibijbijen Jamal, and Nassiri Laila. "Antibacterial Activity of Aqueous Extracts of Anacyclus Pyrethrum (L) Link and Corrigiola Telephiifolia Pourr. From the Middle Atlas Region-Morocco." European Scientific Journal, ESJ 13, no. 33 (November 30, 2017): 116. http://dx.doi.org/10.19044/esj.2017.v13n33p116.

Повний текст джерела

Анотація:

Aqueous extracts of Corrigiola telephiifolia and Anacyclus pyrethrum were studied in vitro for their antimicrobial properties. These plants were collected from the Central Middle Atlas region. Corrigiola telephiifolia Pourr. and Anacyclus pyrethrum (L.) Link are widely used as a natural drug for the treatment of various infectious diseases. Phytochemical screening of the plants showed the presence of flavonoids, saponins, tannins, steroids and alkaloids. The determination of the polyphenols in the aqueous extract of the two plants revealed the presence of significant amounts of polyphenols (58.5 ± 0.76 mg equivalent of gallic acid (GEA) per gram of extract for C. telephiifolia, and 97.57 mg equivalent of gallic acid (GEA) per gram of extract of A. pyrethrum). The antimicrobial activity of the aqueous extracts against three bacterial strains was evaluated on the basis of the inhibition zone using the disk diffusion assay. The aqueous extract of Corrigiola telephiifolia is slightly active on E. coli. At a concentration of extract 100 μg mL-1, the size of the inhibition zone equal to 9 ± 0.06 mm. Whereas S. aureus, E. coli and K. pneumoniae showed a sensitivity to the macerate of Anacyclus pyrethrum at the concentration 100 μg mL-1 of extract with an inhibition zone 16.55 ± 0.6 mm, 14.95 ± 1.25 mm and 10.83 ± 0.96 respectively.

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Desiatkina, Oksana, Serena K. Johns, Nicoleta Anghel, Ghalia Boubaker, Andrew Hemphill, Julien Furrer, and Emilia Păunescu. "Synthesis and Antiparasitic Activity of New Conjugates—Organic Drugs Tethered to Trithiolato-Bridged Dinuclear Ruthenium(II)–Arene Complexes." Inorganics 9, no. 8 (July 21, 2021): 59. http://dx.doi.org/10.3390/inorganics9080059.

Повний текст джерела

Анотація:

Tethering known drugs to a metalorganic moiety is an efficient approach for modulating the anticancer, antibacterial, and antiparasitic activity of organometallic complexes. This study focused on the synthesis and evaluation of new dinuclear ruthenium(II)–arene compounds linked to several antimicrobial compounds such as dapsone, sulfamethoxazole, sulfadiazine, sulfadoxine, triclosan, metronidazole, ciprofloxacin, as well as menadione (a 1,4-naphtoquinone derivative). In a primary screen, 30 compounds (17 hybrid molecules, diruthenium intermediates, and antimicrobials) were assessed for in vitro activity against transgenic T. gondii tachyzoites constitutively expressing β-galactosidase (T. gondii β-gal) at 0.1 and 1 µM. In parallel, the cytotoxicity in noninfected host cells (human foreskin fibroblasts, HFF) was determined by an alamarBlue assay. When assessed at 1 µM, five compounds strongly impaired parasite proliferation by >90%, and HFF viability was retained at 50% or more, and they were further subjected to T. gondii β-gal dose-response studies. Two compounds, notably 11 and 13, amide and ester conjugates with sulfadoxine and metronidazole, exhibited low IC50 (half-maximal inhibitory concentration) values 0.063 and 0.152 µM, and low or intermediate impairment of HFF viability at 2.5 µM (83 and 64%). The nature of the anchored drug as well as that of the linking unit impacted the biological activity.

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Jakób, Michał, Jacek Lubkowski, Barry R. O'Keefe, and Alexander Wlodawer. "Structure of a lectin from the sea musselCrenomytilus grayanus(CGL)." Acta Crystallographica Section F Structural Biology Communications 71, no. 11 (October 24, 2015): 1429–36. http://dx.doi.org/10.1107/s2053230x15019858.

Повний текст джерела

Анотація:

CGL is a 150 amino-acid residue lectin that was originally isolated from the sea musselCrenomytilus grayanus. It is specific for binding GalNAc/Gal-containing carbohydrate moieties and in general does not share sequence homology with other known galectins or lectins. Since CGL displays antibacterial, antifungal and antiviral activities, and interacts with high affinity with mucin-type receptors, which are abundant on some cancer cells, knowledge of its structure is of significant interest. Conditions have been established for the expression, purification and crystallization of a recombinant variant of CGL. The crystal structure of recombinant CGL was determined and refined at a resolution of 2.12 Å. The amino-acid sequence of CGL contains three homologous regions (73% similarity) and the folded protein has a β-trefoil topology. Structural comparison of CGL with the closely related lectin MytiLec allowed description of the glycan-binding pockets.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Kukric, Zoran, Ljiljana Topalic-Trivunovic, Svetlana Pavicic, Mirjana Zabic, Snjezana Matos, and Asima Davidovic. "Total phenolic content, antioxidant and antimicrobial activity of Equisetum arvense L." Chemical Industry and Chemical Engineering Quarterly 19, no. 1 (2013): 37–43. http://dx.doi.org/10.2298/ciceq120327040k.

Повний текст джерела

Анотація:

The antioxidant and antimicrobial activity of Equisetum arvense L. harvested in a polluted urban area were investigated. The total phenolic content determined by a modified Folin-Ciocalteu method was 335.8 mg of GEA/g of the dried ethanol extract. Antioxidant activity determined by FRAP method was 28.7 mM Fe(II)/g of extract, whereas antioxidant activity index for stable DPPH free radicals was 3.86. The results were compared with control antioxidants (gallic acid, vitamin C, BHA and BHT). Antimicrobial activity was tested by monitoring the impact of different extract concentrations through measuring the change in optical density of bacterial suspension over time and by determining MIC and MBC using macrodilution method. E. arvense extract has been effective on the tested microorganisms depending on the concentration and exposure time. The extract had the strongest antibacterial activity on Staphylococcus aureus, with MIC and MBC of 11.14 mg/mL and 22.28 mg/mL respectively, and the weakest effect on Bacillus cereus, with MIC of 89.10 mg/mL.

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Kocev, Alexander, Jacob Melamed, Vladimir Torgov, Leonid Danilov, Vladimir Veselovsky та Inka Brockhausen. "The wclY gene of Escherichia coli serotype O117 encodes an α1,4-glucosyltransferase with strict acceptor specificity but broad donor specificity". Glycobiology 30, № 12 (18 травня 2020): 9003–14. http://dx.doi.org/10.1093/glycob/cwaa045.

Повний текст джерела

Анотація:

Abstract The O antigen of enterotoxigenic Escherichia coli serotype O117 consists of repeating units with the structure [-D-GalNAcβ1-3-L-Rhaα1-4-D-Glcα1-4-D-Galβ1-3-D-GalNAcα1-4]n. A related structure is found in E. coli O107 where Glc is replaced by a GlcNAc residue. The O117 and O107 antigen biosynthesis gene clusters are homologous and reveal the presence of four putative glycosyltransferase (GT) genes, wclW, wclX, wclY and wclZ, but the enzymes have not yet been biochemically characterized. We show here that the His6-tagged WclY protein expressed in E. coli Lemo21(DE3) cells is an α1,4-Glc-transferase that transfers Glc to the Gal moiety of Galβ1-3GalNAcα-OPO3-PO3-phenoxyundecyl as a specific acceptor and that the diphosphate moiety of this acceptor is required. WclY utilized UDP-Glc, TDP-Glc, ADP-Glc, as well as UDP-GlcNAc, UDP-Gal or UDP-GalNAc as donor substrates, suggesting an unusual broad donor specificity. Activity using GDP-Man suggested the presence of a novel Man-transferase in Lemo21(DE3) cells. Mutations of WclY revealed that both Glu residues of the Ex7E motif within the predicted GT domain are essential for activity. High GlcNAc-transferase (GlcNAc-T) activities of WclY were created by mutating Arg194 to Cys. A triple mutant identical to WclY in E. coli O107 was identified as an α1,4 GlcNAc-T. The characterization of WclY opens the door for the development of antibacterial approaches.

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Truong Thi, Thanh Dung, and Tri Nguyen Minh. "Biochemical compositions, antioxidant activity, and in vitro antibacterial activity of extract from wild bitter melon (Momordica charantia var. abbreviata Ser.)." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 4, no. 2 (May 25, 2021): 109–14. http://dx.doi.org/10.47866/2615-9252/vjfc.3787.

Повний текст джерела

Анотація:

Wild bitter melon (Momordica charantia var. abbreviata Ser.) is a type of vine that grows wildly in the mountains and plains in Vietnam. The main use parts of the tree are fruit, stem, and leaf that can be eaten as vegetables. This study aims to (1) identify several bioactive chemical compounds in the stems and leaves of bitter melon trees in the forests in the district of Mang Yang, Gia Lai province; (2) evaluate the nutrient composition, antioxidant activity, and antimicrobial ability of fresh juice and extract with ethanol 70°. The results indicated that fresh juice and ethanol 70° extract currently inhibited the growth of experimental strains of microorganisms, in which ethanol 70o extract gives the highest antimicrobial effect. The qualitative results determined that the chemical components of wide bitter melon consist of flavonoids, saponins, tannins, free reducing sugar, organic acids, starch, and fatty acids as the basis for the standardization of exploitation of medicinal resources this material.

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Hasan, Reham H., and Hasan A. Hasan. "Synergism antibacterial activity for novel synthesized Schiff base ligands and semi-thiosemicarbazones with ß-diketones and 4-aminoantipyrine." Bionatura 7, no. 3 (September 15, 2022): 1–11. http://dx.doi.org/10.21931/rb/2022.07.03.43.

Повний текст джерела

Анотація:

Schiff base ligands were synthesised in this work, the first is (Z)-2-((Z)-3-((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)imino)-1,3-diphenylpropylidene) hydrazine-1-carboxamide, the second is (Z)-2-((1E,5Z,6E)-5-((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)imino)-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-dien-3-ylidene)hydrazine-1-carbothioamide. The Schiff base ligands both were synthesised using the same method (the single pot reaction), using EtOH as the reaction medium and GAA as the catalyst in fixed temperature at 70 0C and reflux for 6 hrs. The starting materials of HL1 were semicarbazide,4-aminoantipyrine and dibenzoyl methane, while the starting materials of HL2 were TSC, 4-AAP and Curcumin. The ligands proved to be bi-dentate ligands that coordinate from the azomethane groups. FT-IR, U.V-Visible, 1H and 13C- NMR, molar conductivity, and magnetic susceptibility were used to identify all the compounds produced. The metal ions used in preparing the complexes in this work were Co(II), Cu(II) and Cr(III). Schiff base and its complexes were evaluated for antibacterial activity against four bacterial strains of Gram-negative (Escherichia coli and Pseudomonas aeruginosa), Gram-positive (Staphylococcus aureus and Bacillus Subtilis) and two types of fungi (Candida albicans and Rhizopus Sporium), the results were positive for all compounds tested. Keywords: Schiff base, Curcumin, 4-aminoantipyrine

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Chainikova, I. N., Yu V. Filippova, B. A. Frolov, N. B. Perunova, E. V. Ivanova, T. A. Bondarenko, T. V. Panfilova, A. D. Zheleznova, Yu A. Sarycheva, and O. V. Bukharin. "MILIACINE INFLUENCE ON THE BIOFILM FORMATION OF BACTERIA." Journal of microbiology, epidemiology and immunobiology, no. 4 (August 28, 2016): 3–9. http://dx.doi.org/10.36233/0372-9311-2016-4-3-9.

Повний текст джерела

Анотація:

Aim. The comparative estimation of miliacine influence on the biofilm formation of bacteria. Materials and methods. The objects of investigation were the clinical isolates of Salmonella enteritidis (28), Salmonella typhimurium (24), Klebsiella pneumoniae (8), Pseudomonas aeruginosa (8) and reference strains of lactobacilli (5) and bifidobacteria (3). Miliacin was obtained from crystals of millet oil. Antibacterial activity of miliacin was detected by the method of serial dilutions. For investigation of biofilms miliacin in 100 and 50 mkg/ml concentrations was used. Miliacin was diluted in Twin-21. Biofilm formation was studied by method of O'Toole G.A., Kolter R. (1998) using spectrophotometer Elx 808 (BioTek, USA). The morphometry of biofilms was conducted by atomic force microscopy with the use of scanning probe microscope SMM-2000. Results. Miliacin and its solvent did not influence the growth of bacteria. Maximum sensivity of biofilms to miliacin was detected in K. pneumoniae and P. aeruginosa, minimal - in S. enteritidis. Miliacin did not influence the biofilm formation in strains of lactobacilli and bifidobacteria. Conclusion. Miliacin in addition to immunotropic activity, detected earlier, can inhibit the biofilms of opportunistic and pathogenic bacteria without influence on the biofilm formation of representatives of usual flora.

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Bastas, Kubilay Kurtulus. "Management of Erwinia amylovora by Potential Bio-Pesticides in vitro and in vivo Conditions." Turkish Journal of Agriculture - Food Science and Technology 8, sp1 (December 11, 2020): 38–45. http://dx.doi.org/10.24925/turjaf.v8isp1.38-45.3933.

Повний текст джерела

Анотація:

Erwinia amylovora, the causative agent of fire blight disease, threatens a lot of species of the Rosaceae family. Antibiotics and copper compounds in chemical applications are most frequently are applied, but these can be phytotoxic and cause resistant strains of the pathogen. In our experiments, 20 herbal materials were tested for their antimicrobial effectiveness against the fire blight pathogen in vitro and in planta. The air-dried plants ground into fine powder and extraction was performed at room temperature by maceration with 80% (v/v) methanol/distilled water. The minimum inhibitory concentration values were determined by using disc diffusion method and streptomycin was used as control in all experiments. Antimicrobial activity was evaluated by measuring the inhibition zones in reference to the pathogen. Among the tested plants, Szygium aromaticum, Thymus vulgaris and Rhus cararia showed a good antibacterial activity and they inhibited the growth of E. amylovora with inhibition zone diameter ranging from 21 to 27 mm at 20% (w/v) in absolute methanol compared to streptomycin (31 mm) in vitro conditions. In vivo tests were performed by using highly virulent E. amylovora isolate (Eak24b, 91%) grown on TSA medium and inoculation on young shoots of 3-year-old Gala variety of apple and Santa Maria variety of pear seedlings at 107 CFU ml-1 density of the pathogen. Disease severity (%) was assessed by by proportion of blighted shoot length to the whole shoot length and also efficacy of the extracts was determined by using Abbott formula. The highest efficacy was obtained by S. aromaticum and T. vulgaris extracts of reducing shoot blight of cv. Gala and cv. Santa Maria by 67.81% - 64-12% and 51.50% - 51.04% ratios, respectively. Obtaining results showed that some medicinal and aromatic plant extracts might be used against fire blight disease as potential new generation chemicals on pome fruits within integrated and organic control programs.

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Butt, Haris, and Kubilay Kurtulus Bastas. "Antagonistic Activity of Bacillus spp. Against Fire Blight Disease In vitro and In planta." Turkish Journal of Agriculture - Food Science and Technology 9, sp (January 6, 2022): 2486–92. http://dx.doi.org/10.24925/turjaf.v9isp.2486-2492.4888.

Повний текст джерела

Анотація:

Fire blight, affecting more than one hundred and thirty species in the Rosaceae, is probably the most destructive disease affecting pear and apple cultivars in many countries. Currently, there are no effective synthetic compounds with systemic properties. Other major problem is the occurrence and spread of strains of Erwinia amylovora with resistance to streptomycin and copper. Taken into consideration the human and environmental health, the use of biocontrol agents either as an alternative or as a supplement within an integrated fire blight management strategy has attracted worldwide attention. In this study, E. amylovora solution of 107 CFU ml-1 was treated with bio-control agents, Bacillus subtilis str. QST 713, B. amyloliquefaciens str. MBI 600 and their mixture (at solution densities of 106, 107 and 108 CFU ml-1 for each one) on Petri dishes, containing King’s B medium and, compared with positive (streptomycin sulphate) and negative (sterile distilled water) controls. In vivo studies were performed on two-year-old apple cv. Gala seedlings grown in 45-cm-diameter pots containing a sterilized mix of soil–sand–peat under controlled greenhouse conditions (85% relative humidity, 25°C temperature and 16h of day light). The plants were irrigated as needed by drip-irrigation and each pot received a mineral solution (NPK: 20–20–20) at 2 g l-1 twice. When plant shoots reached a length of 30-35 cm, bio-control agents, individually and their mixture, were applied to the plants by a hand-sprayer. Obtaining the data, 108 CFU ml-1 of Bacillus spp. suspension mixture showed strongest in vitro antibacterial effect (26mm) among the tested treatments after positive control streptomycin (28.6mm). Parallel to in vitro findings, the mixture was most effective against the pathogen on cv. Gala (66.03%). Findings show that the use of mixture of beneficial microorganisms with individual antagonistic properties against the pathogen can be an effective strategy as a natural alternative to agrochemicals in the scope of good agriculture practices.

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Brinza, Ion, Mohamed A. El Raey, Walaa El-Kashak, Omayma A. Eldahshan, and Lucian Hritcu. "Sweroside Ameliorated Memory Deficits in Scopolamine-Induced Zebrafish (Danio rerio) Model: Involvement of Cholinergic System and Brain Oxidative Stress." Molecules 27, no. 18 (September 11, 2022): 5901. http://dx.doi.org/10.3390/molecules27185901.

Повний текст джерела

Анотація:

Sweroside is a secoiridoid glycoside and belongs to a large group of naturally occurring monoterpenes with glucose sugar attached to C-1 in the pyran ring. Sweroside can promote different biological activities such as antifungal, antibacterial, hepatoprotective, gastroprotective, sedative and antitumor, antioxidant, and neuroprotective activities. Zebrafish were given sweroside (12.79, 8.35, and 13.95 nM) by immersion once daily for 8 days, along with scopolamine (Sco, 100 μM) 30 min before the initiation of the behavioral testing to cause anxiety and memory loss. Employing the novel tank diving test (NTT), the Y-maze, and the novel object recognition test (NOR), anxiety-like reactions and memory-related behaviors were assessed. The following seven groups (n = 10 animals per group) were used: control, Sco (100 μM), sweroside treatment (2.79, 8.35, and 13.95 nM), galantamine (GAL, 2.71 μM as the positive control in Y-maze and NOR tests), and imipramine (IMP, 63.11 μM as the positive control in NTT test). Acetylcholinesterase activity (AChE) and the antioxidant condition of the brains were also evaluated. The structure of sweroside isolated from Schenkia spicata was identified. Treatment with sweroside significantly improved the Sco-induced decrease of the cholinergic system activity and brain oxidative stress. These results suggest that sweroside exerts a significant effect on anxiety and cognitive impairment, driven in part by the modulation of the cholinergic system activity and brain antioxidant action.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Bhat, Akshatha K., Asha Maradka, and Santhosh K. "RAKTAMOKSHANA (BLOODLETTING), A MULTI-DIMENSIONAL PREVENTIVE AND CURATIVE APPROACH IN ORAL DISORDERS: A REVIEW." International Journal of Research in Ayurveda and Pharmacy 13, no. 5 (October 15, 2022): 116–20. http://dx.doi.org/10.7897/2277-4343.1305137.

Повний текст джерела

Анотація:

Mukha is considered one of the essential parts of urdhwajatru because it reflects the body's health by acting as the gateway of the alimentary canal. Fast food culture, unhealthy habits like smoking and improper oral hygiene have caused irreversible damage to human health, resulting in different oral cavity diseases. In Ayurveda, mukharoga has been elaborately explained with its symptoms and treatment, which can be well co-related to present oral cavity diseases mentioned in contemporary science. In our classics, various treatment modalities have been explained for different oral cavity diseases, from lips to throat. Raktamokshana is the primary line of treatment mentioned for mukharogas, followed by pratisarana, kavala, gandusha, shastra karma and oral medications. Acharya Vagbhata highlights the importance of Raktamokshana in mukha, danta, dantamoola and gala roga. Since all these diseases are kapha-rakta in origin, they require frequent bloodletting. Specifically, in mukharoga, bloodletting as a method is indicated in puti aasya, paaka of oshtha. In these diseases, bloodletting should be done by shringa, jalouka, and alabu or by cutting the sira. There are many kinds of research undertaken to study the action of bloodletting using leeches and have found plenty of beneficial actions like analgesic, anti-inflammatory and antibacterial activity. Overall, Raktamokshana is a very effective line of treatment in most of the mukharogas, curing the disease and preventing further recurrence.

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Pusey, P. L., V. O. Stockwell, and D. R. Rudell. "Antibiosis and Acidification by Pantoea agglomerans Strain E325 May Contribute to Suppression of Erwinia amylovora." Phytopathology® 98, no. 10 (October 2008): 1136–43. http://dx.doi.org/10.1094/phyto-98-10-1136.

Повний текст джерела

Анотація:

Pantoea agglomerans strain E325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of Erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. Bacterial modification of pH was evaluated as a possible mechanism by analyzing stigma exudates extracted from ‘Gala’ apple stigmas. The pH values for field samples were only slightly lower than controls, but indicated a range (pH 5 to 6) conducive for antibiotic activity according to subsequent assays. Under low-phosphate and low-pH conditions, an antibacterial product of E325 with high specificity to E. amylovora was effective at low concentrations. A minimum of 20 to 40 ng of a ninhydrin-reactive compound purified using RP-HPLC caused visible inhibition in assays. Activity was heat stable and unaffected by amino acids, iron, or enzymes known to affect antibiotics of P. agglomerans. Antibiosis was diminished, however, under basic conditions, and with increasing phosphate concentrations at pH 6 and 7. Inhibition was not observed in media containing phosphate concentrations commonly used in antibiosis assays. We propose that E325 suppresses the fire blight pathogen not only by competing for nutrients on the stigma, but by producing an antibiotic specific to E. amylovora. Further work is necessary to substantiate that the compound is produced and active on flower stigmas.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Raval, Yash S., Anna Samstag, Cedric Taylor, Guohui Huang, Olin Thompson Mefford, and Tzuen-Rong Jeremy Tzeng. "Assessing the Biocompatibility of Multi-Anchored Glycoconjugate Functionalized Iron Oxide Nanoparticles in a Normal Human Colon Cell Line CCD-18Co." Nanomaterials 11, no. 10 (September 22, 2021): 2465. http://dx.doi.org/10.3390/nano11102465.

Повний текст джерела

Анотація:

We have previously demonstrated that iron oxide nanoparticles with dopamine-anchored heterobifunctional polyethylene oxide (PEO) polymer, namely PEO-IONPs, and bio-functionalized with sialic-acid specific glycoconjugate moiety (Neu5Ac(α2-3)Gal(β1-4)-Glcβ-sp), namely GM3-IONPs, can be effectively used as antibacterial agents against target Escherichia coli. In this study, we evaluated the biocompatibility of PEO-IONPs and GM3-IONPs in a normal human colon cell line CCD-18Co via measuring cell proliferation, membrane integrity, and intracellular adenosine triphosphate (ATP), glutathione GSH, dihydrorhodamine (DHR) 123, and caspase 3/7 levels. PEO-IONPs caused a significant decrease in cell viability at concentrations above 100 μg/mL whereas GM3-IONPs did not cause a significant decrease in cell viability even at the highest dose of 500 μg/mL. The ATP synthase activity of CCD-18Co was significantly diminished in the presence of PEO-IONPs but not GM3-IONPs. PEO-IONPs also compromised the membrane integrity of CCD-18Co. In contrast, cells exposed to GM3-IONPs showed significantly different cell morphology, but with no apparent membrane damage. The interaction of PEO-IONPs or GM3-IONPs with CCD-18Co resulted in a substantial decrease in the intracellular GSH levels in a time- and concentration-dependent manner. Conversely, levels of DHR-123 increased with IONP concentrations. Levels of caspase 3/7 proteins were found to be significantly elevated in cells exposed to PEO-IONPs. Based on the results, we assume GM3-IONPs to be biocompatible with CCD-18Co and could be further evaluated for selective killing of pathogens in vivo.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Buatong, Jirayu, Ajay Mittal, Pimonsri Mittraparp-arthorn, Suriya Palamae, Jirakrit Saetang, and Soottawat Benjakul. "Bactericidal Action of Shrimp Shell Chitooligosaccharide Conjugated with Epigallocatechin Gallate (COS-EGCG) against Listeria monocytogenes." Foods 12, no. 3 (February 2, 2023): 634. http://dx.doi.org/10.3390/foods12030634.

Повний текст джерела

Анотація:

The antibacterial effect of chitooligosaccharide conjugated with five different polyphenols, including catechin (COS-CAT), epigallocatechin gallate (COS-EGCG), gallic acid (COS-GAL), caffeic acid (COS-CAF), and ferulic acid (COS-FER), against Listeria monocytogenes was investigated. Among all the conjugates tested, COS-EGCG showed the highest inhibition toward Listeria monocytogenes, with a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 1024 and 1024 µg/mL, respectively. The COS-EGCG conjugate also had a bactericidal effect on the environmental and clinical strains of L. monocytogenes. The low concentration of COS-EGCG conjugate augmented the formation of biofilm and the growth of L. monocytogenes. Nevertheless, the inhibition of biofilm formation and bacterial growth was achieved when treated with the COS-EGCG conjugate at 2 × MIC for 48 h. In addition, the COS-EGCG conjugate at 2 × MIC had the potential to inactivate the pre-biofilm, and it reduced the production of the extracellular polysaccharides of L. monocytogenes. The COS-EGCG conjugate at the MIC/4 effectively impeded the motility (the swimming and swarming) of L. monocytogenes, with an 85.7–94.3% inhibition, while 100% inhibition was achieved with the MIC. Based on scanning electron microscopic (SEM) images, cell wall damage with numerous pores on the cell surface was observed. Such cell distortion resulted in protein leakage. As a result, COS-EGCG could penetrate into the cell and bind with the DNA backbone. Therefore, the COS-EGCG conjugate could be further developed as a natural antimicrobial agent for inhibiting or controlling L. monocytogenes.

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Chaiwarit, Tanpong, Pornchai Rachtanapun, Nutthapong Kantrong, and Pensak Jantrawut. "Preparation of Clindamycin Hydrochloride Loaded De-Esterified Low-Methoxyl Mango Peel Pectin Film Used as a Topical Drug Delivery System." Polymers 12, no. 5 (April 27, 2020): 1006. http://dx.doi.org/10.3390/polym12051006.

Повний текст джерела

Анотація:

In this study, we aimed to develop a low-mexthoxyl pectin (LMP) from mango peel pectin through a de-esterification method for use as a film forming agent. The prepared de-esterified pectin (DP) was compared to commercial LMP (cLMP) which possessed a 29% degree of esterification (DE). Mango peel pectin was extracted from ripe Nam Dokmai mango peel using the microwave-assisted extraction method. Pectin derived from the mango peel was classified as a high mexthoxyl pectin (79% DE) with 75% of galacturonic acid (GalA) content. A de-esterification experiment was designed by central composite design to plot the surface response curve. Our prepared DP was classified as LMP (DE 29.40%) with 69% GalA. In addition, the Fourier-transform infrared spectrophotometer (FTIR) spectra of the DP were similar to cLMP and the pectin backbone was not changed by the de-esterification process. Strikingly, the cLMP and DP films showed non-significant differences between their physical properties (p > 0.05) with respect to the puncture strength (13.72 N/mm2 and 11.13 N/mm2 for the cLMP and DP films, respectively), percent elongation (2.75% and 2.52% for the cLMP and DP films, respectively), and Young’s modulus (67.69 N/mm2 and 61.79 N/mm2 for the cLMP and DP films, respectively). The de-esterified pectin containing clindamycin HCl (DPC) and low-methoxyl pectin containing clindamycin HCl (cLMPC) films demonstrated 93.47% and 98.79% of drug loading content. The mechanical properties of the cLMPC and DPC films were improved possibly due to their crystal structures and a plasticizing effect of clindamycin HCl loaded into the films. The DPC film exhibited a drug release profile similar to that of the cLMPC film. Our anti-bacterial test of the films found that the cLMPC film showed 41.11 and 76.30 mm inhibitory clear zones against Staphylococcus aureus and Cutibacterium acnes, respectively. The DPC film showed 40.78 and 74.04 mm clear zones against S. aureus and C. acnes, respectively. The antibacterial activities of the cLMPC and DPC films were not significantly different from a commercial clindamycin solution. The results of this study suggest that mango peel pectin can be de-esterified and utilized as an LMP and the de-esterified pectin has the potential for use as a film forming agent, similar to cLMP. In addition, the remarkable use of de-esterified mango peel pectin to prepare films, as shown by our study, holds a great promise as an alternative material for anti-bacterial purposes.

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Imad Abdelhamid EL HACI, Wissame MAZARI, and Fawzia ATIK-BEKKARA. "Effect of Ammodaucus leucotrichus Coss. & Dur. Essential Oil on the Viability of Erythrocytes and its Antiradical Activity Assessment." Journal of Natural Product Research and Applications 1, no. 02 (December 3, 2021): 45–53. http://dx.doi.org/10.46325/jnpra.v1i02.14.

Повний текст джерела

Анотація:

Plants for medicinal purposes are considered as the main source of health care for the majority of the world population. In order to promote medicinal plants in Algeria, the present work aimed to assess the physico-chemical characterization, hemolytic, and antioxidant activities of Ammodaucus leucotrichus essential oil of an endemic plant from south-west of Algeria. The plant was harvested from the region of Bechar (south-west of Algeria). The oil was obtained by hydrodistillation method using Clevenger apparatus. The antioxidant activity of the oil was carried out using DPPH scavenging assay, and its effect on erythrocyte cells was evaluated by measuring the hemolytic degree. The obtained results showed that this oil presented a low antioxidant activity compared to positive control (ascorbic acid). The hemolytic activity was very low since the oil diluted to 1/10, while it was low in the pure state. This fact proves the safety of A. leucotrichus oil, which can explain its use without risk by the indigenous population. REFERENCES: Abu Zarga, M.H., Al-Jaber, H.I., Baba Amer, Z.Y., Sakhrib, L., Al-Qudah, M.A., Alhumaidi, J.Y.G., Abaza, I.F., & Afifi, FU. (2013). Chemical composition, antimicrobialand antitumor activities of essential oil of Ammodaucus leucotrichus growing in Algeria.Journal of Biologically Active Products from Nature, 3, 224–231.http://doi.org/10.1080/22311866.2013.833469.Adorjan, B., & Buchbauer, G. (2010). Biological properties of essential oils: An updatedreview. Flavour and Fragrance Journal, 25, 407–426. http://doi.org/10.1002/ffj.2024.AFNOR. (1992). Association Française de Normalisation «Recueil des normes françaises surles huiles essentielles». II édition, Paris.Amorati, R., Foti, M.C., & Valgimigli, L. (2013). Antioxidant activity of essential oils.Journal of Agricultural and Food Chemistry, 61, 10835–10847.http://doi.org/10.1021/jf403496k.Bakkali, F., Averbeck, S., Averbeck, D., Idaomar, M. (2008). Biological effects of essentialoils- a review. Food and Chemical Toxicology, 46, 446–475.http://doi.org/10.1016/j.fct.2007.09106.Barao Paixao, V.L., & de Carvalho, J.F. (2021). Essential oil therapy in rheumatic diseases:A systematic review. Complementary Therapies in Clinical Practice, 43, 101391.http://doi.org/10.1016/j.ctcp.2021.101391Benhouhou, S. (2005). A Guide to Medicinal plants in North Africa: database on Medicinalplants, IUCN center for Mediterranean cooperation. Mâlaga.Bouaziz, M., Yangui, T., Sayadi, S., & Dhouib, A. (2009). Disinfectant properties of essentialoils from Salvia officinalis L. cultivated in Tunisia. Food and Chemical Toxicology, 47,2755–2760. http://doi.org/10.1016/j.fct.2009.08.005.Burt, S. (2004). Essential oils: their antibacterial properties applications in foods-a review.International Journal of Food Microbiology, 94, 223–253.http://doi.org/10.1016/j.ijfoodmicro.2004.03.022.Conforti, F., Sosa, S., Marrelli, M., Menichini, F., Statti, G.A., Uzunov, D., Tubaro, A., &Menichini, F. (2009). The protective ability of Mediterranean dietary plants against theoxidative damage: The role of radical oxygen species in inflammation and thepolyphenol, flavonoid and sterol contents. Food Chemistry, 112, 587–594.http://doi.org/10.1016/j.foodchem.2008.06.013.Dalar, A., Türker, M., & Konczak, I. (2012). Antioxidant capacity and phenolic constituentsof Malva neglecta Wallr. and Plantago lanceolata L. from Eastern Anatolia region ofTurkey. Journal of Herbal Medicine, 2, 42–51.http://doi.org/10.1016/j.hermed.2012.03.001.Dawidowicz, A.L., Szewczyk, J., & Dybowski, M.P. (2016). Modified application of HSSPME for quality evaluation of essential oil plant materials. Talanta, 146, 195–202.http://doi.org/10.1016/j.talanta.2015.08.043.El Haci, I.A., Bekhechi. C., Atik-Bekkara, F., Mazari, W., Gherib, M., Bighelli, A.,Casanova, J., & Fellix, T. (2014). Antimicrobial activity of Ammodaucus leucotrichusfruit oil from Algerian Sahara. Natural Product Communications, 9, 711–712.http://doi.org/10.1177/1934578X1400900533 Essid, R., Rahali, F.Z., Msaada, K., Sghair, I., Hammami, M., Bouratbine, A., Aoun, K., &Limam, F. (2015). Antileishmanial and cytotoxic potential of essential oils frommedicinal plants in Northern Tunisia. Industrial Crops and Products, 77, 795–802.http://doi.org/10.1016/j.indcrop.2015.09.049.Fillatre, Y., Rondeau, D., Daguin, A., & Communal, P.Y. (2016). A work flow for multiclassdetermination of 256 pesticides in essential oils by liquid chromatography tandem massspectrometry using evaporation and dilution approaches: Application to lavandin, lemonand cypress essential oils. Talanta, 149, 178–186.http://doi.org/10.1016/j.talanta.2015.11.052.Gainza, Y.A., Domingues, L.F., Perez, O.P., Rabelo, M.D., Lopez, E.R., & Chagas, A.C.S.(2015). Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenerviaessential oil from Cuba on Haemonchus contortus. Industrial Crops and Products, 76,647–652. http://doi.org/10.1016/j.indcrop.2015.07.056.Gomes, M.R.F., Schuh, R.S., Jacques, A.L.B., Dorneles, G.G., Montanha, J., Roehe, P.M.,Bordignon, S., Dallegrave, E., Leal, M.B., & Limberger, R.P. (2013). Biologicalassessment (antiviral and antioxidant) and acute toxicity of essential oils from Drimysangustifolia and D. brasiliensis. Revista Brasileira de Farmacognosia, 23, 284–290.http://doi.org/10.1590/S0102-695X2012005000142.Halla, N., Helenoa, S.A., Costa, P., Fernandes, I.P., Calhelha, R.C., Boucherit, K., Rodrigues,A.E., Ferreira, I.C.F.R., & Barreiro, M.F. (2018). Chemical profile and bioactiveproperties of the essential oil isolated from Ammodaucus leucotrichus fruits growing inSahara and its evaluation as a cosmeceutical ingredient. Industrial Crops and Products,119, 249–254. http://doi.org/10.1016/j.indcrop.2018.04.043.Hammiche, V., & Maiza, K. (2006). Traditional medicine in central Sahara: pharmacopoeiaof Tassili N’ajjer. Journal of Ethnopharmacology, 105, 358–367.http://doi.org/10.1016/j.jep.2005.11.028.Hossain, M.H., Shah, M.D., Sang, S.V., & Sakari, M. (2012). Chemical composition andantibacterial properties of the essential oils and crude extracts of Merremia borneensis.Journal of King Saud University –Science, 24, 243–249.http://doi.org/10.1016/j.jksus.2011.03.006.Hussein, G., Miyashiro, H., Nakamura, N., Hattori, M., Kakiuchi, N., & Shimotohno, K.(2000). Inhibitory effects of Sudanese medical plant extracts on hepatitis C virus (HCV)protease. Phytotherapy Research, 14, 510–516. http://doi.org/10.1002/1099-1573(200011)14:7<510::aid-ptr646>3.0.co;2-b.Idm’hand, E., Msanda, F., & Cherifi, K. (2020). Medicinal uses, phytochemistry andpharmacology of Ammodaucus leucotrichus. Clinical Phytoscience, 6,6.http://doi.org/10.1186/s40816-020-0154-7.Khaldi, A., Meddah, B., Moussaoui, A., & Sonnet, P. (2017). Anti-mycotoxin effect andantifungal properties of essential oil from Ammodaucus leucotrichus Coss. & Dur. onAspergillus flavus and Aspergillus ochraceus. Journal of Essential Oil and BearingPlants, 20, 36–44. http://doi.org/10.1080/0972060X.2017.1282840.Lee, J.Y., Cho, P.Y., Kim, T.Y., Kang, S.Y., Song, K.Y., & Hong, S.J. (2002). Hemolyticactivity and developmental expression of pore-forming peptide, clonorin. Biochemicaland Biophysical Research Communications, 296, 1238–1244.http://doi.org/10.1016/s0006-291x(02)02062-4.Manssouri, M., Znini, M., & Majidi, L. (2020). Studies on the antioxidant activity of essentialoil and various extracts of Ammodaucus leucotrichus Coss. & Dur. fruits from Morocco.Journal of Taibah University for Science, 14, 124–130.http://doi.org/10.1080/16583655.2019.1710394. Pavlović, I., Petrović, S., Radenković, M., Milenković, M., Couladis, M., Branković, S.,Pavlović, M.D., & Niketic, D.M. (2012). Composition, antimicrobial, antiradical andspasmolytic activity of Ferula heuffelii Griseb. ex Heuffel (Apiaceae) essential oil. FoodChemistry, 130, 310–315. http://doi.org/10.1016/j.foodchem.2011.07.043.Pillai, S., Mahmud, R., Lee, W.C., & Perumal, S. (2012). Anti-parasitic Activity of Myristicafragrans Houtt. essential oil against Toxoplasma Gondii parasite. APCBEE Procedia, 2,92–96. http://doi.org/10.1016/j.apcbee.2012.06.017.Quezel, P., & Santa, S. (1963). Nouvelle flore de l’Algérie et des régions désertiquesméridionales, CNRS, Paris.Riaz, M., Rasool, N., Bukhari, I.H., Shahid, M., Zubair, M., Rizwan, K., & Rashid, U.(2012). In vitro antimicrobial, antioxidant, cytotoxicity and GC/MS analysis of Mazusgoodenifolius. Molecules, 17, 14275–14287. http://doi.org/10.3390/molecules171214275Sadaoui, N., Bec, N., Barragnan-Montero, V., Kadri, N., Cuisinier, F., Larroque, C., Arab,K., & Khettal, B. (2018). The essential oil of Algerian Ammodaucus leucotrichus Coss.& Dur. and its effect on the cholinesterase and monoamine oxidase activities.Fitoterapia, 130, 1–5. http://doi.org/10.1016/j.fitote.2018.07.015Saleh, M.A., Clark, S., Woodard, B., & Deolu-Sobogun, S.A. (2010). Antioxidant and freeradical scavenging activities of essential oils. Ethnicity and Disease, 20 Suppl 1, 78–82.Salem, M.Z.M., Zidan, Y.E., Mansour, M.A.M., El Hadidi, N.M.N., & Abo Elgat, W.A.A.(2016). Antifungal activities of two essential oils used in the treatment of threecommercial woods deteriorated by five common mold fungi. InternationalBiodeterioration and Biodegradation, 106, 88–96.http://doi.org/10.1016/j.ibiod.2015.10.010.Sebaaly, C., Charcosset, C., Stainmesse, S., Fessi, H., & Greige-Gergesa, H. (2016). Cloveessential oil-in-cyclodextrin-in-liposomes in the aqueous and lyophilized states: fromlaboratory to large scale using a membrane contactor. Carbohydrate Polymers, 138, 75–85. http://doi.org/10.1016/j.carbpol.2015.11.053.Sharma, P., Sharma, J.D. (2001). In vitro hemolysis of human erythrocytes by plant extractswith antiplasmodial activity. Journal of Ethnopharmacology, 74, 239–243.http://doi.org/10.1016/S0378-8741(00)00370-6.Tampieri, M.P., Galuppi, R., Macchioni, F., Carelle, M.S., Falcioni, L., Cioni, P.L., &Morelli, I. (2005). The inhibition of Candida albicans by selected essential oils and theirmajor components. Mycopathologia, 159, 339–345. http://doi.org/10.1007/s11046-003-4790-5.Turek, C., & Stintzing, F.C. (2012). Impact of different storage conditions on the quality ofselected essential oils. Food Research International, 46, 341–353.http://doi.org/10.1016/j.foodres.2011.12.028.Velasco-Negueruela, A., Pérez-Alonso, M.J., Pérez de Paz, P.L., Palá-Paúl, J., & Sanz, J.(2006). Analysis by gas chromatography-mass spectrometry of the volatiles from thefruits of Ammodaucus leucotrichus subsp. leucotrichus and subsp. nanocarpus grown inNorth Africa and the Canary Islands, respectively. Journal of Chromatography A, 1108,273–275. http://doi.org/10.1016/j.chroma.2006.01.031.

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Isha and Neetu Sachan. "Design, Synthesis and Biological Assessment of Thiazole Derivatives as Possible Antioxidant and Antimicrobial Agents." Journal of Pharmaceutical Research International, December 3, 2021, 24–32. http://dx.doi.org/10.9734/jpri/2021/v33i53a33635.

Повний текст джерела

Анотація:

Aims: To synthesize thiazole derivatives and evaluate their therapeutic potential to continue our quest for new antibacterial and antioxidant drugs. Place and Duration of Study: Institute of Pharmaceutical Research, GLA University, Mathura, Uttar Pradesh, between January 2021 and July 2021. Methodology: The target compounds in this investigation were synthesized in the search for new molecules having antioxidant and antimicrobial activity. Physicochemical and spectroanalytical studies validated the derivatives molecular structures. Antioxidant and antimicrobial properties of the synthesized molecules were evaluated in vitro using the DPPH and tube dilution methods, respectively. Results: The majority of the synthesized derivatives displayed antioxidant and antimicrobial activity. The efficacy of the derivatives varied based upon the substituent. Compound 7c exhibited significant antioxidant and antibacterial activity, according to the results of the study. Conclusion: Our results showed the antioxidant and antibacterial properties of novel thiazole compounds, implying the probability of their utilization in the development of new therapeutics.

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Wei, Shuangshuang, Yingxia Zhang, Meidi An, Ran Guo, Shenghong Xie, Jialu Wang, Yanting Song, Rong Wang, and Wenying Jiang. "A Temporin Derived Peptide Showing Antibacterial and Antibiofilm Activities against Staphylococcus aureus." Protein & Peptide Letters 30 (December 2, 2022). http://dx.doi.org/10.2174/0929866530666221202123011.

Повний текст джерела

Анотація:

Background: Temporin is one family of the shortest antimicrobial peptides found in Ranidae frogs. Staphylococcus aureus is one of the main pathogens of suppurative diseases and food contamination, causing severe local or systemic infections in humans. Temporin-GHa (GHa) was previously obtained from Hylarana guentheri, showing weak antibacterial activity against S. aureus. Most temporin peptides are positively charged by arginine and lysine; however, GHa contains histidine. Objective: In order to investigate the impact of positively charged amino acid on its antibacterial and antibiofilm activity, GHa4R was designed and synthesized by replacing histidine with arginine in GHa. Method: The antibacterial activity and efficacy against S. aureus were detected by minimum inhibitory concentration, minimum bactericidal concentration, and time-killing kinetics assays. The action mechanism was determined by propidium iodide uptake and scanning electron microscopy assays. The antibiofilm activity was measured by the MTT method. Eradication of biofilm was observed by fluorescence microscope. Results: Compared to GHa, GHa4R had stronger antibacterial activity and bactericidal efficacy against S. aureus. Impressively, GHa4R presented antibacterial activity against methicillin-resistant S. aureus (MRSA). It was barely affected by temperature, pH, and storage period, showing high stability. Furthermore, it increased the permeability of the cell membrane and damaged the membrane integrity, leading to cell death. In addition, GHa4R did not induce antibiotic resistance in S. aureus in 30 days, but the MIC of vancomycin was doubled. It not only inhibited S. aureus biofilm formation but also eradicated 24 h-biofilms. Conclusion: The above-mentioned characteristics make GHa4R a promising candidate for the treatment of S. aureus infections.

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Zhu, Ruiying, Ran Guo, Chunmei Yu, Xiuchuan Tan, Shuangshuang Wei, Yanting Song, Rong Wang, et al. "Arginine replacement of histidine on temporin-GHa enhances the antimicrobial and antibiofilm efficacy against Staphylococcus aureus." Bioscience, Biotechnology, and Biochemistry, November 11, 2022. http://dx.doi.org/10.1093/bbb/zbac168.

Повний текст джерела

Анотація:

Abstract Antimicrobial peptides (AMPs) show broad-spectrum microbicidal activity against bacteria, fungi and viruses, and have been considered as one of the most promising candidates to overcome the bacterial antimicrobial resistance. Structural modification of AMPs is an effective strategy to develop high-efficiency and low-toxicity antibacterial agents. A series of peptides GHaR6R, GHaR7R, GHaR8R and GHaR9W with arginine replacement of histidine derived from temporin-GHa of Hylarana guentheri were designed and synthesized. These derived peptides exhibit antibacterial activity against Staphylococcus aureus, and GHaR8R exerts bactericidal effect within 15 min at 4 × MIC (25 μM). The derived peptides caused rapid depolarization of bacteria, and the cell membrane damage was monitored using quartz crystal microbalance with dissipation assay, which suggests that they target cell membranes to exert antibacterial effects. The derived peptides can effectively eradicate mature biofilms of S. aureus. Taken together, the derived peptides are promising antibacterial agent candidates against S. aureus.

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Asparinda, Iin, and Tita Juwitaningsih. Acta Pharmaciae Indonesia : Acta Pharm Indo 8, no. 2 (December 29, 2020): 69. http://dx.doi.org/10.20884/1.api.2020.8.2.3487.

Повний текст джерела

Анотація:

Gal manjakani (Quercus Infectoria) is one of the most popular medicinal plants in Asia. This plant is found in Turkey, Syria, Persia, Cyprus, and Greece. This study aims to determine the compounds contained in the non-polar fraction and to determine the antibacterial activity and toxicity of the fraction. Gal Manjakani was extracted by maceration method with acetone then fractionated with n-hexane, ethyl acetate and methanol. Non-polar fractions were analyzed using Gas Chromatography-Mass Spectroscopy (GCMS). The antibacterial activity test used disc diffusion and microdilution methods based on the CLSI standard method against Staphylococcus aureus and Escherichia coli bacteria. Toxicity test was conducted using the Brine Shrimp Lethality Test (BSLT) method. The GC-MS identification results showed that the non-polar fraction of gal manjakani contained 27 compounds, with the main compounds being cis-vaccenic acid (35.19%), n-hexadecanoic acid (16.66%), 1-heptadecan carboxylic acid (5.03%), dodecanoic acid. (4.73%), bis (2-ethylhexyl) phthalate (4.35%), hexadecanoic acid, methyl ester (2.14%) and tetradecanoic acid (1.31%). The results of the antibacterial activity test showed activity with an inhibition zone of 6.26 ± 0.6 mm against S.aureus bacteria and 8.23 ± 0.21 mm against E.coli bacteria, with a percentage of effectiveness against S.aureus bacteria of 15.34% and for E. coli 44.24%. The MIC and MBC values of S.aureus bacteria were> 5000 (µg / mL) and> 5000 (µg / mL) and E. coli was 312.5 (µg / mL) and> 5000 (µg / mL). The results of the toxicity test showed that the non-polar fraction of gal Manjakani was toxic with an LC50 value of 2.1527 ppm.

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Jing, Yongshuai, Wenjing Cheng, Yunfeng Ma, Yameng Zhang, Mingsong Li, Yuguang Zheng, Danshen Zhang, and Lanfang Wu. "Structural Characterization, Antioxidant and Antibacterial Activities of a Novel Polysaccharide From Zingiber officinale and Its Application in Synthesis of Silver Nanoparticles." Frontiers in Nutrition 9 (June 3, 2022). http://dx.doi.org/10.3389/fnut.2022.917094.

Повний текст джерела

Анотація:

A novel polysaccharide (ZOP) was extracted from Zingiber officinale with ultrasonic assisted extraction method. ZOP monosaccharide composition and mole ratio is GlcA: GalA: Glc: Gal: Ara = 1.97:1.15:94.33:1.48:1.07. Then, the particle size of ZOP-NPs prepared by nano-precipitation method was 230.5 nm, and the polydispersity index (PDI) was 0.260. Using ZOP and ZOP-NPs as reductants and stabilizers, ZOP-AgNPs and ZOP-NPs-AgNPs were prepared. They were characterized by ultraviolet-visible spectrophotometer (UV-Vis), fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), transmission electron microscope (TEM), and X-ray diffraction (XRD). The silver chelation rate of polysaccharide silver nanoparticles (AgNPs) ranged from 68.70 to 82.12%. ZOP-AgNPs (0.5%, w/v; 1%, w/v) and ZOP-NPs-AgNPs (0.5%, w/v; 1%, w/v) exhibited a narrow particle size distribution of 31.1, 34.6, 25.1 and 27.6 nm, respectively. And the zeta potential values of them were−19.4,−21.6,−19.7,−23.8mV, respectively. The antioxidant and antibacterial activities of ZOP-NPs-AgNPs were superior to those of ZOP, ZOP-NPs and ZOP-AgNPs.

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Abdelshafy, Asem Mahmoud, Eid, A. El-Naggar, and Mohamed, N. Kenawi. "Promotion of Antioxidant and Antibacterial Activities of Fermented Oat Products." Asian Journal of Applied Chemistry Research, December 27, 2021, 31–38. http://dx.doi.org/10.9734/ajacr/2021/v10i3-430239.

Повний текст джерела

Анотація:

Fermentation of oats by probiotics provides higher nutritional value and can be considered as a significant source of bioactive compounds for the human body. Moringa leaves powder (MLP) at the levels of 0.25 and 0.50% were used as an additional prebiotic source to supply oat fermentation by Lactobacillus plantaram ATCC 14917 and Lactobacillus delbrueckii ssp. Bulgaricus EMCC 11102. The results indicated that oat products supplemented with MLP (0.50%) and fermented by L. delbrueckii ssp. Bulgaricus EMCC 11102 showed the highest values of free phenolic content and antioxidant activity (30.87 mg Gallic acid (GAL) /100 g and 7.64%, respectively), followed by oat products supplemented with MLP at level 0.50% and fermented by L. plantaram (28.38 mg GAL /100 g and 5.31%, respectively). Also, oat products fermented by probiotics showed different antibacterial activity by well-diffusion agar method against selected pathogenic bacteria. It is thus concluded that supplementation of fermented oat products with MLP will improve the nutritional value and health benefits of fermented oat products.

Стилі APA, Harvard, Vancouver, ISO та ін.

48

GEA, SAHARMAN, KHATARINA MELDAWATI PASARIBU, APPEALWAN ALTRUISTIS SARUMAHA, and SRI RAHAYU. "Cassava starch/bacterial cellulose-based bioplastics with Zanthoxylum acanthopodium." Biodiversitas Journal of Biological Diversity 23, no. 5 (May 4, 2022). http://dx.doi.org/10.13057/biodiv/d230542.

Повний текст джерела

Анотація:

Abstract. Gea S, Pasaribu KM, Sarumaha AA, Rahayu S. 2022. Cassava starch/bacterial cellulose-based bioplastics with Zanthoxylum acanthopodium. Biodiversitas 23: 2601-2608. The use of conventional plastics has become one of the biggest environmental problems because of their difficulties in decomposing. Bioplastics are plastics that are decompose easily in nature as they are naturally sourced. The purpose of this research was to create antimicrobial bioplastics from cassava starch with the addition of bacterial cellulose (BC) as a reinforcement material and Zanthoxylum acanthopodium (andaliman) as an antibacterial agent. Furthermore, this study determines the optimal concentration of BC and the antibacterial effect of Z. acanthopodium extract in bioplastics produced by the solution casting method. The addition of BC to bioplastics changed the properties of bioplastics, according to FTIR, XRD, TGA, SEM, and tensile strength analysis. Mechanical analysis showed an increase in tensile strength with higher amount of BC. The best tensile strength was observed in CS2BCA sample (2.34 MPa). The antibacterial test of bioplastic samples showed good inhibition zone (10.8 mm) against Bacillus cereus.

Стилі APA, Harvard, Vancouver, ISO та ін.

49

Tran, Minh T. L. "Isolation and characterization of antibacterial compounds from Euphorbia tirucalli against Xanthomonas axonopodis pv. citri." Journal of Agriculture and Development 20, no. 6 (December 30, 2021). http://dx.doi.org/10.52997/jad.1.06.2021.

Повний текст джерела

Анотація:

Xanthomonas axonopodis pv. citri (X. axonopodis pv. citri) is the cause of canker disease on lime trees that negatively aﬀect plant health and fruit quality. This study focused on the comparison of the extraction yield and antibacterial properties of Euphorbia tirucalli against X. axonopodis pv. citri, phytochemical screening, quantifcation of phenolic and ﬂavonoid contents of the fraction extract. The results showed that the ethyl acetate fraction (EA) (7.5 mg/mL) of E. tirucalli from Dak Nong province had the best activity against bacteria with diameter of inhibition zone determined 15.50 ± 0.50 mm, and the minimum inhibition concentration was 0.312 mg/mL. Alkaloids, ﬂavonoids, tannins, and terpenoids were found in the EA fraction extract of E. tirucalli, whereas saponin did not appear in the extract. The phenolic and ﬂavonoid content was in the range of 14.46 - 98.63 mg GEA/g and 90.34 - 408.86 µg QE/g, respectively. Column chromatography followed Nuclear Magnetic Resonance spectra were performed and the three compounds were identifed as scopoletin, gallic acid, and 3,3’,4’-tri - O - methylellagic acid. This study suggests that the extract from E. tirucalli and the isolated compounds can be used for managing of citrus canker disease.

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Nugraha, Alexander Patera, I. Gusti Aju Wahju Ardani, Ratri Maya Sitalaksmi, Nastiti Faradilla Ramadhani, Desi Rachmayanti, Dina Kumala, Viol Dhea Kharisma, et al. "Anti–Peri-implantitis Bacteria's Ability of Robusta Green Coffee Bean (Coffea Canephora) Ethanol Extract: An In Silico and In Vitro Study." European Journal of Dentistry, September 8, 2022. http://dx.doi.org/10.1055/s-0042-1750803.

Повний текст джерела

Анотація:

Abstract Objective This study was aimed to investigate RGCBE extract as antioxidant and anti–peri-implantitis bacteria through in vitro study and its potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic through in silico study. Materials and Methods Absorption, distribution, metabolism, excretion and toxicity prediction, molecular docking simulation, and visualization of chlorogenic acid (CGA) and coumaric acid (CA) as anti-inflammatory, antioxidant, and antibacterial were investigated in silico. Inhibition zone by diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of RGCBE extract against Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), and Prevotella intermedia (Pi) were done. Statistical Analysis the analysis of variance (ANOVA) difference test, and the post-hoc Tukey's Honest Significant Different (HSD) with a different significance value of p<0.05 Results GCA and CA compounds are good drug molecules and it has low toxicity. Chlorogenic acid have higher binding activity than coumaric acid to tumor necrosis factor (TNF)-α, nuclear factor (NF)-κB, receptor activation NF-κB (RANK) and its ligand (RANKL), interleukin (IL)-6, IL-10, runt related transcription factor (RUNX2), receptor activator nuclear Kappa beta Ligand-osteoprotegrin osteocalcin (RANKL-OPG), osteocalcin, nuclear factor associated T-cell 1 (NFATc1), tartate resistant acid phosphatase (TRAP), peptidoglycan, flagellin, dectin, Hsp70, and Hsp10 protein. RGCB ethanol extract has high antioxidant ability and it has MIC, MBC, and inhibit the growth of Aa, Pg, Fn, and Pi at 50% concentration with significantly different (p=0.0001 and<0.05). Conclusion RGCB ethanol extract has high antioxidant ability and 50% RGCB ethanol extract may act as strong anti–peri-implantitis bacteria in vitro. In addition, CGA in RGCB potential as antioxidant, antibacterial, anti-inflammatory, antibone resorption, and proosteogenic in silico.

Стилі APA, Harvard, Vancouver, ISO та ін.

Статті в журналах з теми "Antibacterial gla"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями