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Pandey, Bharat Chandra, and Dr Sangita Gupta. "Effectiveness of Algae in Wastewater Treatment Systems." International Journal for Research in Applied Science and Engineering Technology 10, no. 8 (August 31, 2022): 520–26. http://dx.doi.org/10.22214/ijraset.2022.46241.
Повний текст джерелаАнотація:
Abstract: Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD) play important role in determining the quality of wastewater. Hence it is necessary to calculate COD and BOD of water before setting up of wastewater treatment plant. Algae has been used for decades for various purposes. It is one of the important characteristics is to nitrogen, phosphorus etc., which are harmful for drinking and other purposes but they act as food for algae. Thus in this study COD and BOD analysis is done for sterilized and non-sterilized wastewater after and before treating it with algae in inverse fluidization under aerobic condition, for different time interval and found that percentage reduction in COD and BOD for sterilized wastewater gives greater value than non-sterilized water the reason for this difference being the decrease in the competition between algae and other micro-organism which are present in raw wastewater and COD % reduction is 65-70 % and BOD % reduction is 68.75- 70.5%.Organic and inorganic substances which are released into the environment as a result of domestic, agricultural and industrial water activities lead to pollution.
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Vu, Diep, Shaokai Gao, Tyler Berte, Mary Kacarab, Qi Yao, Kambiz Vafai, and Akua Asa-Awuku. "External and internal cloud condensation nuclei (CCN) mixtures: controlled laboratory studies of varying mixing states." Atmospheric Measurement Techniques 12, no. 8 (August 8, 2019): 4277–89. http://dx.doi.org/10.5194/amt-12-4277-2019.
Повний текст джерелаАнотація:
Abstract. Changes in aerosol chemical mixtures modify cloud condensation nuclei (CCN) activity. Previous studies have developed CCN models and validated changes in external and internal mixing state with ambient field data. Here, we develop an experimental method to test and validate the CCN activation of known aerosol chemical composition with multicomponent mixtures and varying mixing states. CCN activation curves consisting of one or more activation points are presented. Specifically, simplified two-component systems of varying hygroscopicity were generated under internal, external, and transitional mixing conditions. κ-Köhler theory predictions were calculated for different organic and inorganic mixtures and compared to experimentally derived kappa values and respective mixing states. This work employs novel experimental methods to provide information on the shifts in CCN activation data due to external to internal particle mixing from controlled laboratory sources. Results show that activation curves consisting of single and double activation points are consistent with internal and external mixtures, respectively. In addition, the height of the plateau at the activation points is reflective of the externally mixed concentration in the mixture. The presence of a plateau indicates that CCN activation curves consisting of multiple inflection points are externally mixed aerosols of varying water-uptake properties. The plateau disappears when mixing is promoted in the flow tube. At the end of the flow tube experiment, the aerosols are internally mixed and the CCN activated fraction data can be fit with a single-sigmoid curve. The technique to mimic externally to internally mixed aerosol is applied to non-hygroscopic carbonaceous aerosol with organic and inorganic components. To our knowledge, this work is the first to show controlled CCN activation of mixed non-hygroscopic soot with hygroscopic material as the aerosol population transitions from externally to internally mixed states in laboratory conditions. Results confirm that CCN activation analysis methods used here and in ambient data sets are robust and may be used to infer the mixing state of complex aerosol compositions of unknown origin.
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Calsolaro, Federica, Katia Martina, Elisa Borfecchia, Fernando Chávez-Rivas, Giancarlo Cravotto та Gloria Berlier. "β-Cyclodextrin-Silica Hybrid: A Spatially Controllable Anchoring Strategy for Cu(II)/Cu(I) Complex Immobilization". Catalysts 10, № 10 (27 вересня 2020): 1118. http://dx.doi.org/10.3390/catal10101118.
Повний текст джерелаАнотація:
The development of new strategies for spatially controllable immobilization has encouraged the preparation of novel catalysts based on the organic-inorganic hybrid concept. In the present paper, a Cu-based multi-structured silica catalyst has been prepared and fully characterized. The inclusion of Cu(II) in β-cyclodextrins has been exploited with the double aim to stabilize the metal and to act as a source of Cu(I) catalytic sites. Multi-technique characterization by infrared, UV-visible, electron microscopy and X-ray absorption spectroscopies of the fresh and exhaust catalysts provided information on the local structure, redox properties and stability of the investigated hybrid systems. The catalytic system showed that copper nanospecies were dispersed on the support and hardly affected by the catalytic tests, confirming the stabilizing effect of β-CD, and likely of the N1-(3-Trimethoxysilylpropyl) diethylenetriamine spacer, as deduced by X-ray absorption spectroscopy analysis. Overall, we demonstrate a feasible approach to efficiently anchor Cu(II) species and to obtain a reusable single-site hybrid catalyst well suited for Cu(I)-catalyzed alkyne-azide cycloaddition.
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Andriulo, Fabrizio, Rodorico Giorgi, Calin Constantin Steindal, Hartmut Kutzke, Susan Braovac, and Piero Baglioni. "Hybrid nanocomposites made of diol-modified silanes and nanostructured calcium hydroxide. Applications to Alum-treated wood." Pure and Applied Chemistry 89, no. 1 (January 1, 2017): 29–39. http://dx.doi.org/10.1515/pac-2016-1014.
Повний текст джерелаАнотація:
AbstractThe alum-treated Viking Age archaeological wooden objects from the Oseberg find have undergone extensive chemical deterioration due to the original conservation treatment, based on alum salts (KAl(SO4)2·12H2O), done in the early 1900s. Today, the artifacts are highly acidic (pH≤2) and fragile; in some cases wood has almost completely lost its structural integrity. Research on conservation methods for these finds is currently underway. In the present study, organic/inorganic multi-functional ‘hybrid systems’ – using propylene glycol modified TEOS and alkaline nanoparticles (Ca(OH)2) – have been engineered to deacidify and consolidate alum-treated wood in a single step. The advantage of using silicon monomer and nano-materials as a starting point resides in their ease of penetration into the wood structure, where silicon monomers subsequently undergo polymerization. Treated samples were investigated using thermal analysis (DTG), X-Ray Diffraction (XRD), infrared spectroscopy (ATR-FTIR), SEM/EDX and Gas Chromatography Mass Spectroscopy (GC-MS). Results suggest that in addition to consolidating the wood, alkoxysilanes could act as a bridging agent between remaining lignin and calcium hydroxide nanoparticles.
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Shi, Qingya, Fen Pei, Gary A. Silverman, Stephen C. Pak, David H. Perlmutter, Bing Liu, and Ivet Bahar. "Mechanisms of Action of Autophagy Modulators Dissected by Quantitative Systems Pharmacology Analysis." International Journal of Molecular Sciences 21, no. 8 (April 19, 2020): 2855. http://dx.doi.org/10.3390/ijms21082855.
Повний текст джерелаАнотація:
Autophagy plays an essential role in cell survival/death and functioning. Modulation of autophagy has been recognized as a promising therapeutic strategy against diseases/disorders associated with uncontrolled growth or accumulation of biomolecular aggregates, organelles, or cells including those caused by cancer, aging, neurodegeneration, and liver diseases such as α1-antitrypsin deficiency. Numerous pharmacological agents that enhance or suppress autophagy have been discovered. However, their molecular mechanisms of action are far from clear. Here, we collected a set of 225 autophagy modulators and carried out a comprehensive quantitative systems pharmacology (QSP) analysis of their targets using both existing databases and predictions made by our machine learning algorithm. Autophagy modulators include several highly promiscuous drugs (e.g., artenimol and olanzapine acting as activators, fostamatinib as an inhibitor, or melatonin as a dual-modulator) as well as selected drugs that uniquely target specific proteins (~30% of modulators). They are mediated by three layers of regulation: (i) pathways involving core autophagy-related (ATG) proteins such as mTOR, AKT, and AMPK; (ii) upstream signaling events that regulate the activity of ATG pathways such as calcium-, cAMP-, and MAPK-signaling pathways; and (iii) transcription factors regulating the expression of ATG proteins such as TFEB, TFE3, HIF-1, FoxO, and NF-κB. Our results suggest that PKA serves as a linker, bridging various signal transduction events and autophagy. These new insights contribute to a better assessment of the mechanism of action of autophagy modulators as well as their side effects, development of novel polypharmacological strategies, and identification of drug repurposing opportunities.
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Niedek, Christopher R., Fan Mei, Maria A. Zawadowicz, Zihua Zhu, Beat Schmid, and Qi Zhang. "Quantitative chemical assay of nanogram-level particulate matter using aerosol mass spectrometry: characterization of particles collected from uncrewed atmospheric measurement platforms." Atmospheric Measurement Techniques 16, no. 4 (February 23, 2023): 955–68. http://dx.doi.org/10.5194/amt-16-955-2023.
Повний текст джерелаАнотація:
Abstract. Aerosol generation techniques have expanded the utility of aerosol mass spectrometry (AMS) for offline chemical analysis of airborne particles and droplets. However, standard aerosolization techniques require relatively large liquid volumes (e.g., several milliliters) and high sample masses that limit their utility. Here we report the development and characterization of a micronebulization AMS (MN-AMS) technique that requires as low as 10 µL of sample and can provide the quantification of the nanogram level of organic and inorganic substances via the usage of an isotopically labeled internal standard (34SO42-). Using standard solutions, the detection limits for this technique were determined at 0.19, 0.75, and 2.2 ng for sulfate, nitrate, and organics, respectively. The analytical recoveries for these species are 104 %, 87 %, and 94 %, respectively. This MN-AMS technique was applied successfully to analyze filter and impactor samples collected using miniature particulate matter (PM) samplers deployable on uncrewed atmospheric measurement platforms, such as uncrewed aerial systems (UASs) and tethered balloon systems (TBSs). Chemical composition of PM samples collected from a UAS field campaign conducted at the Department of Energy's (DOE) Southern Great Plains (SGP) observatory was characterized. The offline MN-AMS data compared well with the in situ PM composition measured by a co-located aerosol chemical speciation monitor (ACSM). In addition, the MN-AMS and ion chromatography (IC) agreed well for measurements of sulfate and nitrate concentrations in the PM extracts. This study demonstrates the utility of combining MN-AMS with uncrewed measurement platforms to provide quantitative measurements of ambient PM composition.
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Bai, Naling, Hanlin Zhang, Shuangxi Li, Xianqing Zheng, Juanqin Zhang, Haiyun Zhang, Sheng Zhou, Huifeng Sun, and Weiguang Lv. "Long-term effects of straw and straw-derived biochar on soil aggregation and fungal community in a rice–wheat rotation system." PeerJ 6 (January 4, 2019): e6171. http://dx.doi.org/10.7717/peerj.6171.
Повний текст джерелаАнотація:
Background Soil aggregation is fundamental for soil functioning and agricultural productivity. Aggregate formation depends on microbial activity influencing the production of exudates and hyphae, which in turn act as binding materials. Fungi are also important for improving soil quality and promoting plant growth in a symbiotic manner. There is a scarcity of findings comparing the long-term impacts of different yearly double-crop straw return modes (e.g., straw return to the field and straw-derived biochar return to the field) on soil aggregation and fungal community structure in rice–wheat rotation systems. Methods The effects of 6-year continuous straw and straw-derived biochar amendment on soil physicochemical properties and the fungal community were evaluated in an intensively managed crop rotation system (rice–wheat). Soil samples of different aggregates (macroaggregates, microaggregates, and silt clay) from four different fertilization regimes (control, CK; traditional inorganic fertilization, CF; straw returned to field, CS; straw-derived biochar addition, CB) were obtained, and Illumina MiSeq sequencing analysis of the fungal internal transcribed spacer gene was performed. Results Compared to CF, CS and CB enhanced soil organic carbon, total nitrogen, and aggregation in 0–20 and 20–40 cm soil, with CB exhibiting a stronger effect. Additionally, agrowaste addition increased the mean weight diameter and the geometric diameter and decreased the fractal dimension (p < 0.05). Principal coordinates analysis indicated that fertilization management affected fungal community structure and aggregation distribution. In addition, CS increased fungal community richness and diversity, compared to CK, CB decreased these aspects. Ascomycota, unclassified_k_Fungi, and Basidiomycota were the dominant phyla in all soil samples. At the genus level, CB clearly increased fungi decomposing biosolids (Articulospora in macroaggregates in 0–20 cm soil and Neurospora in macroaggregates in 20–40 cm soil); decreased pathogenic fungi (Monographella in macroaggregates and Gibberella in microaggregates in 0–20 cm soil) and CO2-emission-related fungi (Pyrenochaetopsis in microaggregates and silt clay in 0–40 cm soil) (p < 0.05). Straw and biochar with inorganic fertilizer counteracted some of the adverse effects of the inorganic fertilizer with biochar showing better effects than straw.
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Gutowski, Łukasz, Kaja Gutowska, Maria Pioruńska-Stolzmann, Piotr Formanowicz, and Dorota Formanowicz. "Systems Approach to Study Associations between OxLDL and Abdominal Aortic Aneurysms." International Journal of Molecular Sciences 20, no. 16 (August 11, 2019): 3909. http://dx.doi.org/10.3390/ijms20163909.
Повний текст джерелаАнотація:
Although abdominal aortic aneurysm (AAA) is a common vascular disease and is associated with high mortality, the full pathogenesis of AAA remains unknown to researchers. Abdominal aortic aneurysms and atherosclerosis are strongly related. Currently, it is more often suggested that development of AAA is not a result of atherosclerosis, however, individual factors can act independently or synergistically with atherosclerosis. One of such factors is low-density lipoprotein (LDL) and its oxidized form (oxLDL). It is known that oxLDL plays an important role in the pathogenesis of atherosclerosis, thus, we decided to examine oxLDL impact on the development of AAA by creating two models using Petri-nets. The first, full model, contains subprocess of LDL oxidation and all subprocesses in which it participates, while the second, reduced model, does not contain them. The analysis of such models can be based on t-invariants. They correspond to subprocesses which do not change the state of the modeled system. Moreover, the knockout analysis has been used to estimate how crucial a selected transition (representing elementary subprocess) is, based on the number of excluded subprocesses as a result of its knockout. The results of the analysis of our models show that oxLDL affects 55.84% of subprocesses related to AAA development, but the analysis of the nets based on knockouts and simulation has shown that the influence of oxLDL on enlargement and rupture of AAA is negligible.
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Vilella-Figuerola, Alba, Alex Gallinat, Rafael Escate, Sònia Mirabet, Teresa Padró, and Lina Badimon. "Systems Biology in Chronic Heart Failure—Identification of Potential miRNA Regulators." International Journal of Molecular Sciences 23, no. 23 (December 3, 2022): 15226. http://dx.doi.org/10.3390/ijms232315226.
Повний текст джерелаАнотація:
Heart failure (HF) is a complex disease entity with high clinical impact, poorly understood pathophysiology and scantly known miRNA-mediated epigenetic regulation. We have analysed miRNA patterns in patients with chronic HF (cHF) and a sex- and age-matched reference group and pursued an in silico system biology analysis to discern pathways involved in cHF pathophysiology. Twenty-eight miRNAs were identified in cHF that were up-regulated in the reference group, and eight of them were validated by RT-qPCR. In silico analysis of predicted targets by STRING protein-protein interaction networks revealed eight cluster networks (involving seven of the identified miRNAs) enriched in pathways related to cell cycle, Ras, chemokine, PI3K-AKT and TGF-β signaling. By ROC curve analysis, combined probabilities of these seven miRNAs (let-7a-5p, miR-107, miR-125a-5p, miR-139-5p, miR-150-5p, miR-30b-5p and miR-342-3p; clusters 1–4 [C:1–4]), discriminated between HF with preserved ejection fraction (HFpEF) and HF with reduced ejection fraction (HFrEF), and ischaemic and non-ischaemic aetiology. A combination of miR-107, miR-139-5p and miR-150-5p, involved in clusters 5 and 7 (C:5+7), discriminated HFpEF from HFrEF. Pathway enrichment analysis of miRNAs present in C:1–4 (let-7a-5p, miR-125a-5p, miR-30b-5p and miR-342-3p) revealed pathways related to HF pathogenesis. In conclusion, we have identified a differential signature of down-regulated miRNAs in the plasma of HF patients and propose novel cellular mechanisms involved in cHF pathogenesis.
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Mamedov, R. A., M. A. Allanazarova, R. R. Sagdeev, and T. N. Suyunbaev. "Formation conditions and evolution of the oil and gas strata of the prospective East Siberian oil and gas province." Proceedings of higher educational establishments. Geology and Exploration, no. 1 (June 22, 2022): 38–49. http://dx.doi.org/10.32454/0016-7762-2022-64-1-38-49.
Повний текст джерелаАнотація:
Introduction. East Siberian Sea shelf refers to the Novosibirsk-Chukotka prospective oil and gas province. The definition of the East Siberian shelf as a prospective oil and gas province and its division into areas is based, along with the structural and geological prerequisites, on the identification of numerous bitumen occurrences in the Paleozoic, Triassic and Jurassic sediments of the Novosibirsk Islands.Aim. To construct spatio-temporal digital models of sedimentary basins and hydrocarbon systems for the main horizons of source rocks, as well as to carry out their detailed analysis based on the available information about the oil and gas content, the gas-chemical composition of sediments, the characteristics of the component composition and evolution of source rocks within the studied prospective oil and gas province. The conducted research made it possible to study regional trends in oil and gas content, features of the sedimentary cover formation and the development of hydrocarbon systems in the area under study.Materials and methods. The materials of production reports obtained for individual large objects in the water area were the source of information. A basin analysis was based on a model developed by the Equinor specialists (Somme et al., 2018) [8, 9], which covered the time period from the Triassic to Paleogene inclusive and took into account the plate-tectonic reconstructions performed by Dor’e et al. in 2015. The resulting model included four main sedimentary complexes: pre-Aptian, Apt-Upper Cretaceous, Paleogene, and Neogene-Quaternary.Results. The calculation of numerical models was carried out in two versions with different types of kerogen of oil and gas source strata corresponding to humic and sapropel organic matter. The key factor in controlling the development of hydrocarbon systems was found to be the sinking rate of the basins and the thickness of the formed overburden complexes.Conclusion. The conducted analysis allowed the most promising research objects to be identified. The main foci of hydrocarbon generation in the Aptian-Late Cretaceous and Paleogene complexes were identified, along with the area of their most probable accumulation. Significant hydrocarbon potential is expected in the Paleogene clinoforms of the Eastern Arctic. This complex is currently underestimated, thus requiring further resource assessment study. A detailed mapping of its interior structure should be carried out.
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Kerimov, V. Yu, Yu V. Shcherbina, and A. A. Ivanov. "Formation conditions and evolution of oil and gas source strata of the Laptev sea shelf ore and gas province." Proceedings of higher educational establishments. Geology and Exploration, no. 3 (February 28, 2021): 46–59. http://dx.doi.org/10.32454/0016-7762-2020-63-3-46-59.
Повний текст джерелаАнотація:
Introduction. To date, no unified well-established concepts have been developed regarding the oil and gas geological zoning of the Laptev Sea shelf, as well as other seas of the Eastern Arctic. Different groups of researchers define this region either as an independently promising oil and gas region [7, 8], or as a potential oil and gas basin [1].Aim. To construct spatio-temporal digital models of sedimentary basins and hydrocarbon systems for the main horizons of oil and gas source rocks. A detailed analysis of information on oil and gas content, the gas chemical study of sediments, the characteristics of the component composition and thermal regime of the Laptev sea shelf water area raises the question on the conditions for the formation and evolution of oil and gas source strata within the studied promising oil and gas province. The conducted research made it possible to study the regional trends in oil and gas content, the features of the sedimentary cover formation and the development of hydrocarbon systems in the area under study.Materials and methods. The materials of production reports obtained for individual large objects in the water area were the source of initial information. The basin analysis was based on a model developed by Equinor specialists (Somme et al., 2018) [14—17], covering the time period from the Triassic to Paleogene inclusive and taking into account the plate-tectonic reconstructions. The resulting model included four main sedimentary complexes: pre-Aptian, Apt-Upper Cretaceous, Paleogene, and Neogene-Quaternary.Results. The calculation of numerical models was carried out in two versions with different types of kerogen from the oil and gas source strata corresponding to humic and sapropel organic matter. The results obtained indicated that the key factor controlling the development of hydrocarbon systems was the sinking rate of the basins and the thickness of formed overburden complexes, as well as the geothermal field of the Laptev Sea.Conclusion. The analysis of the results obtained allowed the most promising research objects to be identified. The main foci of hydrocarbon generation in the Paleogene and Neogene complexes and the areas of the most probable accumulation were determined. Significant hydrocarbon potential is expected in the Paleogene clinoforms of the Eastern Arctic.
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Qaswar, Muhammad, Jing Huang, Waqas Ahmed, Dongchu Li, Shujun Liu, Sehrish Ali, Kailou Liu, et al. "Long-Term Green Manure Rotations Improve Soil Biochemical Properties, Yield Sustainability and Nutrient Balances in Acidic Paddy Soil under a Rice-Based Cropping System." Agronomy 9, no. 12 (November 20, 2019): 780. http://dx.doi.org/10.3390/agronomy9120780.
Повний текст джерелаАнотація:
Cultivation of green manure (GM) crops in intensive cropping systems is important for enhancing crop productivity through soil quality improvement. We investigated yield sustainability, nutrient stocks, nutrient balances and enzyme activities affected by different long-term (1982–2016) green manure rotations in acidic paddy soil in a double-rice cropping system. We selected four treatments from a long-term experiment, including (1) rice-rice-winter fallow as a control treatment (R-R-F), (2) rice-rice-milkvetch (R-R-M), (3) rice-rice-rapeseed (R-R-R), and (4) rice-rice-ryegrass (R-R-G). The results showed that different GM rotations increased grain yield and the sustainable yield index compared with those of the R-R-F treatment. Compared with those of R-R-F, the average grain yield of early rice in R-R-M, R-R-R, and R-R-G increased by 45%, 29%, and 27%, respectively and that of late rice increased by 46%, 28%, and 26%, respectively. Over the years, grain yield increased in all treatments except R-R-F. Green manure also improved the soil chemical properties (SOM and total and available N and P), except soil pH, compared to those of the control treatment. During the 1983–1990 cultivation period, the soil pH of the R-R-M treatment was lower than that of the R-R-F treatment. The addition of green manure did not mitigate the soil acidification caused by the use of inorganic fertilizers. The soil organic matter (SOM), total nitrogen (TN) and total phosphorus (TP) contents and stocks of C, N and P increased over the years. Furthermore, GM significantly increased phosphatase and urease activities and decreased the apparent N and P balances compared with those in the winter fallow treatment. Variance partitioning analysis revealed that soil properties, cropping systems, and climatic factors significantly influenced annual grain yield. Aggregated boosted tree (ABT) analysis quantified the relative influences of the different soil properties on annual grain yield and showed that the relative influences of TN content, SOM, pH, and TP content on annual crop yield were 27.8%, 25.7%, 22.9%, and 20.7%, respectively. In conclusion, GM rotation is beneficial for sustaining high crop yields by improving soil biochemical properties and reducing N and P balances in acidic soil under double- rice cropping systems.
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Roman, Pawel, Martijn F. M. Bijmans, and Albert J. H. Janssen. "Quantification of individual polysulfides in lab-scale and full-scale desulfurisation bioreactors." Environmental Chemistry 11, no. 6 (2014): 702. http://dx.doi.org/10.1071/en14128.
Повний текст джерелаАнотація:
Environmental context Emission into the atmosphere of gaseous streams containing sulfur compounds, such as H2S and SOx, will lead to the unwanted formation of acid rain. In order to prevent this, biological processes can be employed to treat sulfur-containing gas streams. In this study, we describe a way to investigate the speciation of polysulfide anions in biodesulfurisation systems, which might enable further understanding and development of these processes. Abstract Environmental pollution caused by the combustion of fuel sources containing inorganic and organic sulfur compounds such as hydrogen sulfide (H2S) and thiols, is a global issue as it leads to SO2 emissions. To remove H2S from gas streams such as liquefied petroleum gas (LPG), biological processes can be applied. In these processes, polysulfide anions (Sx2–) play a significant role as they enhance the dissolution of H2S and act as intermediates in the biological oxidation of hydrogen sulfide ions to elemental sulfur. Despite their important role, the distribution of the various polysulfide species in full-scale biodesulfurisation systems has not yet been reported. With conventionally applied spectrophotometric analysis it is only possible to determine the total concentration of Sx2–. Moreover, this method is very sensitive to matrix effects. In this paper, we apply a method that relies on the derivatisation of Sx2– to dimethyl polysulfanes. Owing to the instability of higher dimethyl polysulfanes (Me2S4 to Me2S8), standards are not commercially available and had to be prepared by us. We present a simplified quantification method for higher dimethyl polysulfanes by calculating high performance liquid chromatogaphy (HPLC) UV response factors based on the addition of internal standards. The method was subsequently used to assess the distribution of polysulfide anions in both a laboratory-scale and a full-scale biodesulfurisation unit. We found that the average chain length of polysulfides strongly depends on the process conditions and a maximum of 5.33 sulfur atoms per polysulfide molecule was measured. Results of this study are required by mechanistic and kinetic models that attempt to describe product selectivity of sulfide oxidising bioreactors.
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Marosi, Mate, Miroslav N. Nenov, Jessica Di Re, Nolan M. Dvorak, Musaad Alshammari, and Fernanda Laezza. "Inhibition of the Akt/PKB Kinase Increases Nav1.6-Mediated Currents and Neuronal Excitability in CA1 Hippocampal Pyramidal Neurons." International Journal of Molecular Sciences 23, no. 3 (February 1, 2022): 1700. http://dx.doi.org/10.3390/ijms23031700.
Повний текст джерелаАнотація:
In neurons, changes in Akt activity have been detected in response to the stimulation of transmembrane receptors. However, the mechanisms that lead to changes in neuronal function upon Akt inhibition are still poorly understood. In the present study, we interrogate how Akt inhibition could affect the activity of the neuronal Nav channels with while impacting intrinsic excitability. To that end, we employed voltage-clamp electrophysiological recordings in heterologous cells expressing the Nav1.6 channel isoform and in hippocampal CA1 pyramidal neurons in the presence of triciribine, an inhibitor of Akt. We showed that in both systems, Akt inhibition resulted in a potentiation of peak transient Na+ current (INa) density. Akt inhibition correspondingly led to an increase in the action potential firing of the CA1 pyramidal neurons that was accompanied by a decrease in the action potential current threshold. Complementary confocal analysis in the CA1 pyramidal neurons showed that the inhibition of Akt is associated with the lengthening of Nav1.6 fluorescent intensity along the axonal initial segment (AIS), providing a mechanism for augmented neuronal excitability. Taken together, these findings provide evidence that Akt-mediated signal transduction might affect neuronal excitability in a Nav1.6-dependent manner.
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Křen, Vladimír. "Chirality Matters: Biological Activity of Optically Pure Silybin and Its Congeners." International Journal of Molecular Sciences 22, no. 15 (July 23, 2021): 7885. http://dx.doi.org/10.3390/ijms22157885.
Повний текст джерелаАнотація:
This review focuses on the specific biological effects of optically pure silymarin flavo-nolignans, mainly silybins A and B, isosilybins A and B, silychristins A and B, and their 2,3-dehydro derivatives. The chirality of these flavonolignans is also discussed in terms of their analysis, preparative separation and chemical reactions. We demonstrated the specific activities of the respective diastereomers of flavonolignans and also the enantiomers of their 2,3-dehydro derivatives in the 3D anisotropic systems typically represented by biological systems. In vivo, silymarin flavonolignans do not act as redox antioxidants, but they play a role as specific ligands of biological targets, according to the “lock-and-key” concept. Estrogenic, antidiabetic, anticancer, antiviral, and antiparasitic effects have been demonstrated in optically pure flavonolignans. Potential application of pure flavonolignans has also been shown in cardiovascular and neurological diseases. Inhibition of drug-metabolizing enzymes and modulation of multidrug resistance activity by these compounds are discussed in detail. The future of “silymarin applications” lies in the use of optically pure components that can be applied directly or used as valuable lead structures, and in the exploration of their true molecular effects.
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Engelberg, Shira, Yuexi Lin, Yehuda G. Assaraf, and Yoav D. Livney. "Targeted Nanoparticles Harboring Jasmine-Oil-Entrapped Paclitaxel for Elimination of Lung Cancer Cells." International Journal of Molecular Sciences 22, no. 3 (January 20, 2021): 1019. http://dx.doi.org/10.3390/ijms22031019.
Повний текст джерелаАнотація:
Selectively targeted drug delivery systems are preferable chemotherapeutic platforms, as they specifically deliver the drug cargo into tumor cells, while minimizing untoward toxic effects. However, these delivery systems suffer from insufficient encapsulation efficiency (EE), encapsulation capacity (EC), and premature drug release. Herein, we coencapsulated paclitaxel (PTX) and Jasmine oil (JO) within PEG-PCL nanoparticles (NPs), with an average diameter < 50 nm, selectively targeted to non-small cell lung cancer (NSCLC) cells, via S15-aptamer (APT) decoration. JO was selected as an “adhesive” oily core to enhance PTX entrapment, as JO and PTX share similar hydrophobicity and terpenoid structure. JO markedly enhanced EE of PTX from 23% to 87.8% and EC from 35 ± 6 to 74 ± 8 µg PTX/mg PEG-PCL. JO also markedly increased the residual amount of PTX after 69 h, from 18.3% to 65%. Moreover, PTX cytotoxicity against human NSCLC A549 cells was significantly enhanced due to the co-encapsulation with JO; the IC50 value for PTX encapsulated within JO-containing APT-NPs was 20-fold lower than that for APT-NPs lacking JO. Remarkably, JO-containing APT-NPs displayed a 6-fold more potent cell-killing, relatively to the free-drug. Collectively, these findings reveal a marked synergistic contribution of JO to the cytotoxic activity of APT-NP-based systems, for targeted PTX delivery against NSCLC, which may be readily applied to various hydrophobic chemotherapeutics.
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Casati, Sara, Chiara Giannasi, Stefania Niada, Roberta F. Bergamaschi, Marica Orioli, and Anna T. Brini. "Bioactive Lipids in MSCs Biology: State of the Art and Role in Inflammation." International Journal of Molecular Sciences 22, no. 3 (February 2, 2021): 1481. http://dx.doi.org/10.3390/ijms22031481.
Повний текст джерелаАнотація:
Lipidomics is a lipid-targeted metabolomics approach that aims to the comprehensive analysis of lipids in biological systems in order to highlight the specific functions of lipid species in health and disease. Lipids play pivotal roles as they are major structural components of the cellular membranes and energy storage molecules but also, as most recently shown, they act as functional and regulatory components of intra- and intercellular signaling. Herein, emphasis is given to the recently highlighted roles of specific bioactive lipids species, as polyunsaturated fatty acids (PUFA)-derived mediators (generally known as eicosanoids), endocannabinoids (eCBs), and lysophospholipids (LPLs), and their involvement in the mesenchymal stem cells (MSCs)-related inflammatory scenario. Indeed, MSCs are a heterogenous population of multipotent cells that have attracted much attention for their potential in regulating inflammation, immunomodulatory capabilities, and reparative roles. The lipidomics of the inflammatory disease osteoarthritis (OA) and the influence of MSCs-derived lipids have also been addressed.
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Go, Gyeongyun, Chang-Seuk Lee, Yeo Min Yoon, Ji Ho Lim, Tae Hyun Kim, and Sang Hun Lee. "PrPC Aptamer Conjugated–Gold Nanoparticles for Targeted Delivery of Doxorubicin to Colorectal Cancer Cells." International Journal of Molecular Sciences 22, no. 4 (February 17, 2021): 1976. http://dx.doi.org/10.3390/ijms22041976.
Повний текст джерелаАнотація:
Anticancer drugs, such as fluorouracil (5-FU), oxaliplatin, and doxorubicin (Dox) are commonly used to treat colorectal cancer (CRC); however, owing to their low response rate and adverse effects, the development of efficient drug delivery systems (DDSs) is required. The cellular prion protein PrPC, which is a cell surface glycoprotein, has been demonstrated to be overexpressed in CRC, however, there has been no research on the development of PrPC-targeting DDSs for targeted drug delivery to CRC. In this study, PrPC aptamer (Apt)-conjugated gold nanoparticles (AuNPs) were synthesized for targeted delivery of Dox to CRC. Thiol-terminated PrPC-Apt was conjugated to AuNPs, followed by hybridization of its complementary DNA for drug loading. Finally, Dox was loaded onto the AuNPs to synthesize PrPC-Apt-functionalized doxorubicin-oligomer-AuNPs (PrPC-Apt DOA). The PrPC-Apt DOA were spherical nanoparticles with an average diameter of 20 nm. Treatment of CRC cells with PrPC-Apt DOA induced reactive oxygen species generation by decreasing catalase and superoxide dismutase activities. In addition, treatment with PrPC-Apt DOA inhibited mitochondrial functions by decreasing the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, complex 4 activity, and oxygen consumption rates. Compared to free Dox, PrPC-Apt DOA decreased proliferation and increased apoptosis of CRC cells to a greater degree. In this study, we demonstrated that PrPC-Apt DOA targeting could effectively deliver Dox to CRC cells. PrPC-Apt DOA can be used as a treatment for CRC, and have the potential to replace existing anticancer drugs, such as 5-FU, oxaliplatin, and Dox.
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Rumyantseva, Marina, Abulkosim Nasriddinov, Svetlana Vladimirova, Sergey Tokarev, Olga Fedorova, Ivan Krylov, Konstantin Drozdov, Alexander Baranchikov, and Alexander Gaskov. "Photosensitive Organic-Inorganic Hybrid Materials for Room Temperature Gas Sensor Applications." Nanomaterials 8, no. 9 (August 29, 2018): 671. http://dx.doi.org/10.3390/nano8090671.
Повний текст джерелаАнотація:
In this work, the hybrids based on nanocrystalline SnO2 or In2O3 semiconductor matrixes and heterocyclic Ru(II) complex are studied as materials for gas sensors operating at room temperature under photoactivation with visible light. Nanocrystalline semiconductor oxides are obtained by chemical precipitation with subsequent thermal annealing and characterized by XRD, SEM and single-point BET methods. The heterocyclic Ru(II) complex is synthesized for the first time and investigated by 1H NMR, 13C NMR APT, MALDI-MS analysis, and UV-Vis spectroscopy. The HOMO and LUMO energies of the Ru(II) complex are calculated from cyclic voltammetry data. The hybrid materials are characterized by TGA-MS analysis and EDX mapping. The optical properties of hybrids are studied by UV-Vis spectroscopy in the diffuse reflection mode. The investigation of spectral dependencies of photoconductivity of hybrid samples demonstrates that the role of organic dye consists in shifting the photosensitivity range towards longer wavelengths. Sensor measurements demonstrate that hybrid materials are able to detect NO2 in the concentration range of 0.25–2 ppm without the use of thermal heating under periodic illumination with even low-energy long-wavelength (red) light.
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Jiang, Chenhao, Zijian Liu, Jingsheng Yuan, Zhenru Wu, Lingxiang Kong, Jiayin Yang, and Tao Lv. "Construction of Two Independent RAB Family-Based Scoring Systems Based on Machine Learning Algorithms and Definition of RAB13 as a Novel Therapeutic Target for Hepatocellular Carcinoma." International Journal of Molecular Sciences 24, no. 5 (February 22, 2023): 4335. http://dx.doi.org/10.3390/ijms24054335.
Повний текст джерелаАнотація:
Hepatocellular carcinoma (HCC) remains a global health challenge with a low early diagnosis rate and high mortality. The Rab GTPase (RAB) family plays an essential role in the occurrence and progression of HCC. Nonetheless, a comprehensive and systematic investigation of the RAB family has yet to be performed in HCC. We comprehensively assessed the expression landscape and prognostic significance of the RAB family in HCC and systematically correlated these RAB family genes with tumor microenvironment (TME) characteristics. Then, three RAB subtypes with distinct TME characteristics were determined. Using a machine learning algorithm, we further established a RAB score to quantify TME features and immune responses of individual tumors. Moreover, to better evaluate patient prognosis, we established a RAB risk score as an independent prognostic factor for patients with HCC. The risk models were validated in independent HCC cohorts and distinct HCC subgroups, and their complementary advantages guided clinical practice. Furthermore, we further confirmed that the knockdown of RAB13, a pivotal gene in risk models, suppressed HCC cell proliferation and metastasis by inhibiting the PI3K/AKT signaling pathway, CDK1/CDK4 expression, and epithelial-mesenchymal transition. In addition, RAB13 inhibited the activation of JAK2/STAT3 signaling and the expression of IRF1/IRF4. More importantly, we confirmed that RAB13 knockdown enhanced GPX4-dependent ferroptosis vulnerability, highlighting RAB13 as a potential therapeutic target. Overall, this work revealed that the RAB family played an integral role in forming HCC heterogeneity and complexity. RAB family-based integrative analysis contributed to enhancing our understanding of the TME and guided more effective immunotherapy and prognostic evaluation.
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Christodoulou, Christiana C., and Eleni Zamba Papanicolaou. "Integrated Bioinformatics Analysis of Shared Genes, miRNA, Biological Pathways and Their Potential Role as Therapeutic Targets in Huntington’s Disease Stages." International Journal of Molecular Sciences 24, no. 5 (March 2, 2023): 4873. http://dx.doi.org/10.3390/ijms24054873.
Повний текст джерелаАнотація:
Huntington’s Disease (HD) is a progressive neurodegenerative disease caused by CAG repeat expansion in the huntingtin gene (HTT). The HTT gene was the first disease-associated gene mapped to a chromosome, but the pathophysiological mechanisms, genes, proteins or miRNAs involved in HD remain poorly understood. Systems bioinformatics approaches can divulge the synergistic relationships of multiple omics data and their integration, and thus provide a holistic approach to understanding diseases. The purpose of this study was to identify the differentially expressed genes (DEGs), HD-related gene targets, pathways and miRNAs in HD and, more specifically, between the pre-symptomatic and symptomatic HD stages. Three publicly available HD datasets were analysed to obtain DEGs for each HD stage from each dataset. In addition, three databases were used to obtain HD-related gene targets. The shared gene targets between the three public databases were compared, and clustering analysis was performed on the common shared genes. Enrichment analysis was performed on (i) DEGs identified for each HD stage in each dataset, (ii) gene targets from the public databases and (iii) the clustering analysis results. Furthermore, the hub genes shared between the public databases and the HD DEGs were identified, and topological network parameters were applied. Identification of HD-related miRNAs and their gene targets was obtained, and a miRNA-gene network was constructed. Enriched pathways identified for the 128 common genes revealed pathways linked to multiple neurodegeneration diseases (HD, Parkinson’s disease, Spinocerebellar ataxia), MAPK and HIF-1 signalling pathways. Eighteen HD-related hub genes were identified based on network topological analysis of MCC, degree and closeness. The highest-ranked genes were FoxO3 and CASP3, CASP3 and MAP2 were found for betweenness and eccentricity and CREBBP and PPARGC1A were identified for the clustering coefficient. The miRNA-gene network identified eleven miRNAs (mir-19a-3p, mir-34b-3p, mir-128-5p, mir-196a-5p, mir-34a-5p, mir-338-3p, mir-23a-3p and mir-214-3p) and eight genes (ITPR1, CASP3, GRIN2A, FoxO3, TGM2, CREBBP, MTHFR and PPARGC1A). Our work revealed that various biological pathways seem to be involved in HD either during the pre-symptomatic or symptomatic stages of HD. This may offer some clues for the molecular mechanisms, pathways and cellular components underlying HD and how these may act as potential therapeutic targets for HD.
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Janiak, Agnieszka, Miroslaw Kwasniewski, Marta Sowa, Anetta Kuczyńska, Krzysztof Mikołajczak, Piotr Ogrodowicz, and Iwona Szarejko. "Insights into Barley Root Transcriptome under Mild Drought Stress with an Emphasis on Gene Expression Regulatory Mechanisms." International Journal of Molecular Sciences 20, no. 24 (December 5, 2019): 6139. http://dx.doi.org/10.3390/ijms20246139.
Повний текст джерелаАнотація:
Root systems play a pivotal role in coupling with drought stress, which is accompanied with a substantial transcriptome rebuilding in the root tissues. Here, we present the results of global gene expression profiling of roots of two barley genotypes with contrasting abilities to cope with drought that were subjected to a mild level of the stress. We concentrate our analysis on gene expression regulation processes, which allowed the identification of 88 genes from 39 families involved in transcriptional regulation in roots upon mild drought. They include 13 genes encoding transcription factors (TFs) from AP2 family represented by ERFs, DREB, or B3 domain-containing TFs, eight WRKYs, six NACs, five of the HD-domain, MYB or MYB-related, bHLH and bZIP TFs. Also, the representatives of C3H, CPP, GRAS, LOB-domain, TCP, Tiffy, Tubby, and NF-Ys TFs, among others were found to be regulated by the mild drought in barley roots. We found that drought tolerance is accompanied with a lower number of gene expression changes than the amount observed in a susceptible genotype. The better drought acclimation may be related to the activation of transcription factors involved in the maintenance of primary root growth and in the epigenetic control of chromatin and DNA methylation. In addition, our analysis pointed to fives TFs from ERF, LOB, NAC, WRKY and bHLH families that may be important in the mild but not the severe drought response of barley roots.
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Lopez-Tello, Jorge, Maria Angeles Jimenez-Martinez, Esteban Salazar-Petres, Ritik Patel, Amy L. George, Richard G. Kay, and Amanda N. Sferruzzi-Perri. "Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy." International Journal of Molecular Sciences 23, no. 11 (June 3, 2022): 6287. http://dx.doi.org/10.3390/ijms23116287.
Повний текст джерелаАнотація:
Pregnancy is characterized by adaptations in the function of several maternal body systems that ensure the development of the fetus whilst maintaining health of the mother. The renal system is responsible for water and electrolyte balance, as well as waste removal. Thus, it is imperative that structural and functional changes occur in the kidney during pregnancy. However, our knowledge of the precise morphological and molecular mechanisms occurring in the kidney during pregnancy is still very limited. Here, we investigated the changes occurring in the mouse kidney during pregnancy by performing an integrated analysis involving histology, gene and protein expression assays, mass spectrometry profiling and bioinformatics. Data from non-pregnant and pregnant mice were used to identify critical signalling pathways mediating changes in the maternal kidneys. We observed an expansion of renal medulla due to proliferation and infiltration of interstitial cellular constituents, as well as alterations in the activity of key cellular signalling pathways (e.g., AKT, AMPK and MAPKs) and genes involved in cell growth/metabolism (e.g., Cdc6, Foxm1 and Rb1) in the kidneys during pregnancy. We also generated plasma and urine proteomic profiles, identifying unique proteins in pregnancy. These proteins could be used to monitor and study potential mechanisms of renal adaptations during pregnancy and disease.
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Ikeshima-Kataoka, Hiroko, Chikatoshi Sugimoto, and Tatsuya Tsubokawa. "Integrin Signaling in the Central Nervous System in Animals and Human Brain Diseases." International Journal of Molecular Sciences 23, no. 3 (January 27, 2022): 1435. http://dx.doi.org/10.3390/ijms23031435.
Повний текст джерелаАнотація:
The integrin family is involved in various biological functions, including cell proliferation, differentiation and migration, and also in the pathogenesis of disease. Integrins are multifunctional receptors that exist as heterodimers composed of α and β subunits and bind to various ligands, including extracellular matrix (ECM) proteins; they are found in many animals, not only vertebrates (e.g., mouse, rat, and teleost fish), but also invertebrates (e.g., planarian flatworm, fruit fly, nematodes, and cephalopods), which are used for research on genetics and social behaviors or as models for human diseases. In the present paper, we describe the results of a phylogenetic tree analysis of the integrin family among these species. We summarize integrin signaling in teleost fish, which serves as an excellent model for the study of regenerative systems and possesses the ability for replacing missing tissues, especially in the central nervous system, which has not been demonstrated in mammals. In addition, functions of astrocytes and reactive astrocytes, which contain neuroprotective subpopulations that act in concert with the ECM proteins tenascin C and osteopontin via integrin are also reviewed. Drug development research using integrin as a therapeutic target could result in breakthroughs for the treatment of neurodegenerative diseases and brain injury in mammals.
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Calin, Bogdan Stefanita, and Irina Alexandra Paun. "A Review on Stimuli-Actuated 3D Micro/Nanostructures for Tissue Engineering and the Potential of Laser-Direct Writing via Two-Photon Polymerization for Structure Fabrication." International Journal of Molecular Sciences 23, no. 22 (November 17, 2022): 14270. http://dx.doi.org/10.3390/ijms232214270.
Повний текст джерелаАнотація:
In this review, we present the most recent and relevant research that has been done regarding the fabrication of 3D micro/nanostructures for tissue engineering applications. First, we make an overview of 3D micro/nanostructures that act as backbone constructs where the seeded cells can attach, proliferate and differentiate towards the formation of new tissue. Then, we describe the fabrication of 3D micro/nanostructures that are able to control the cellular processes leading to faster tissue regeneration, by actuation using topographical, mechanical, chemical, electric or magnetic stimuli. An in-depth analysis of the actuation of the 3D micro/nanostructures using each of the above-mentioned stimuli for controlling the behavior of the seeded cells is provided. For each type of stimulus, a particular recent application is presented and discussed, such as controlling the cell proliferation and avoiding the formation of a necrotic core (topographic stimulation), controlling the cell adhesion (nanostructuring), supporting the cell differentiation via nuclei deformation (mechanical stimulation), improving the osteogenesis (chemical and magnetic stimulation), controlled drug-delivery systems (electric stimulation) and fastening tissue formation (magnetic stimulation). The existing techniques used for the fabrication of such stimuli-actuated 3D micro/nanostructures, are briefly summarized. Special attention is dedicated to structures’ fabrication using laser-assisted technologies. The performances of stimuli-actuated 3D micro/nanostructures fabricated by laser-direct writing via two-photon polymerization are particularly emphasized.
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Varadwaj, Arpita, Pradeep R. Varadwaj, Helder M. Marques, and Koichi Yamashita. "The Pnictogen Bond: The Covalently Bound Arsenic Atom in Molecular Entities in Crystals as a Pnictogen Bond Donor." Molecules 27, no. 11 (May 25, 2022): 3421. http://dx.doi.org/10.3390/molecules27113421.
Повний текст джерелаАнотація:
In chemical systems, the arsenic-centered pnictogen bond, or simply the arsenic bond, occurs when there is evidence of a net attractive interaction between the electrophilic region associated with a covalently or coordinately bound arsenic atom in a molecular entity and a nucleophile in another or the same molecular entity. It is the third member of the family of pnictogen bonds formed by the third atom of the pnictogen family, Group 15 of the periodic table, and is an inter- or intramolecular noncovalent interaction. In this overview, we present several illustrative crystal structures deposited into the Cambridge Structure Database (CSD) and the Inorganic Chemistry Structural Database (ICSD) during the last and current centuries to demonstrate that the arsenic atom in molecular entities has a significant ability to act as an electrophilic agent to make an attractive engagement with nucleophiles when in close vicinity, thereby forming σ-hole or π-hole interactions, and hence driving (in part, at least) the overall stability of the system’s crystalline phase. This overview does not include results from theoretical simulations reported by others as none of them address the signatory details of As-centered pnictogen bonds. Rather, we aimed at highlighting the interaction modes of arsenic-centered σ- and π-holes in the rationale design of crystal lattices to demonstrate that such interactions are abundant in crystalline materials, but care has to be taken to identify them as is usually done with the much more widely known noncovalent interactions in chemical systems, halogen bonding and hydrogen bonding. We also demonstrate that As-centered pnictogen bonds are usually accompanied by other primary and secondary interactions, which reinforce their occurrence and strength in most of the crystal structures illustrated. A statistical analysis of structures deposited into the CSD was performed for each interaction type As···D (D = N, O, S, Se, Te, F, Cl, Br, I, arene’s π system), thus providing insight into the typical nature of As···D interaction distances and ∠R–As···D bond angles of these interactions in crystals, where R is the remainder of the molecular entity.
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Takács-Lovász, Krisztina, József Kun, Timea Aczél, Péter Urbán, Attila Gyenesei, Kata Bölcskei, Éva Szőke, and Zsuzsanna Helyes. "PACAP-38 Induces Transcriptomic Changes in Rat Trigeminal Ganglion Cells Related to Neuroinflammation and Altered Mitochondrial Function Presumably via PAC1/VPAC2 Receptor-Independent Mechanism." International Journal of Molecular Sciences 23, no. 4 (February 14, 2022): 2120. http://dx.doi.org/10.3390/ijms23042120.
Повний текст джерелаАнотація:
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a broadly expressed neuropeptide which has diverse effects in both the peripheral and central nervous systems. While its neuroprotective effects have been shown in a variety of disease models, both animal and human data support the role of PACAP in migraine generation. Both PACAP and its truncated derivative PACAP(6-38) increased calcium influx in rat trigeminal ganglia (TG) primary sensory neurons in most experimental settings. PACAP(6-38), however, has been described as an antagonist for PACAP type I (known as PAC1), and Vasoactive Intestinal Polypeptide Receptor 2 (also known as VPAC2) receptors. Here, we aimed to compare the signaling pathways induced by the two peptides using transcriptomic analysis. Rat trigeminal ganglion cell cultures were incubated with 1 µM PACAP-38 or PACAP(6-38). Six hours later RNA was isolated, next-generation RNA sequencing was performed and transcriptomic changes were analyzed to identify differentially expressed genes. Functional analysis was performed for gene annotation using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome databases. We found 200 common differentially expressed (DE) genes for these two neuropeptides. Both PACAP-38 and PACAP(6-38) treatments caused significant downregulation of NADH: ubiquinone oxidoreductase subunit B6 and upregulation of transient receptor potential cation channel, subfamily M, member 8. The common signaling pathways induced by both peptides indicate that they act on the same target, suggesting that PACAP activates trigeminal primary sensory neurons via a mechanism independent of the identified and cloned PAC1/VPAC2 receptor, either via another target structure or a different splice variant of PAC1/VPAC2 receptors. Identification of the target could help to understand key mechanisms of migraine.
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Luo, Yung-Hung, Kung-Hao Liang, Hsu-Ching Huang, Chia-I. Shen, Chi-Lu Chiang, Mong-Lien Wang, Shih-Hwa Chiou, and Yuh-Min Chen. "State-of-the-Art Molecular Oncology of Lung Cancer in Taiwan." International Journal of Molecular Sciences 23, no. 13 (June 24, 2022): 7037. http://dx.doi.org/10.3390/ijms23137037.
Повний текст джерелаАнотація:
Lung cancers are life-threatening malignancies that cause great healthcare burdens in Taiwan and worldwide. The 5-year survival rate for Taiwanese patients with lung cancer is approximately 29%, an unsatisfactorily low number that remains to be improved. We first reviewed the molecular epidemiology derived from a deep proteogenomic resource in Taiwan. The nuclear factor erythroid 2-related factor 2 (NRF2)antioxidant mechanism was discovered to mediate the oncogenesis and tumor progression of lung adenocarcinoma. Additionally, DNA replication, glycolysis and stress response are positively associated with tumor stages, while cell-to-cell communication, signaling, integrin, G protein coupled receptors, ion channels and adaptive immunity are negatively associated with tumor stages. Three patient subgroups were discovered based on the clustering analysis of protein abundance in tumors. The first subgroup is associated with more advanced cancer stages and visceral pleural invasion, as well as higher mutation burdens. The second subgroup is associated with EGFR L858R mutations. The third subgroup is associated with PI3K/AKT pathways and cell cycles. Both EGFR and PI3K/AKT signaling pathways have been shown to induce NRF2 activation and tumor cell proliferation. We also reviewed the clinical evidence of patient outcomes in Taiwan given various approved targeted therapies, such as EGFR-tyrosine kinase inhibitors and anaplastic lymphoma kinase (ALK)inhibitors, in accordance with the patients’ characteristics. Somatic mutations occurred in EGFR, KRAS, HER2 and BRAF genes, and these mutations have been detected in 55.7%, 5.2%, 2.0% and 0.7% patients, respectively. The EGFR mutation is the most prevalent targetable mutation in Taiwan. EML4-ALK translocations have been found in 9.8% of patients with wild-type EGFR. The molecular profiling of advanced NSCLC is critical to optimal therapeutic decision-making. The patient characteristics, such as mutation profiles, protein expression profiles, drug-resistance profiles, molecular oncogenic mechanisms and patient subgroup systems together offer new strategies for personalized treatments and patient care.
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Koike, Hirofumi, Minoru Morikawa, Hideki Ishimaru, Reiko Ideguchi, Masataka Uetani, Takeshi Hiu, Takayuki Matsuo, and Mitsuharu Miyoshi. "Quantitative Chemical Exchange Saturation Transfer Imaging of Amide Proton Transfer Differentiates between Cerebellopontine Angle Schwannoma and Meningioma: Preliminary Results." International Journal of Molecular Sciences 23, no. 17 (September 5, 2022): 10187. http://dx.doi.org/10.3390/ijms231710187.
Повний текст джерелаАнотація:
Vestibular schwannomas are the most common tumor at the common cerebellopontine angle, followed by meningiomas. Differentiation of these tumors is critical because of the different surgical approaches required for treatment. Recent studies have demonstrated the utility of amide proton transfer (APT)-chemical exchange saturation transfer (CEST) imaging in evaluating malignant brain tumors. However, APT imaging has not been applied in benign tumors. Here, we explored the potential of APT in differentiating between schwannomas and meningiomas at the cerebellopontine angle. We retrospectively evaluated nine patients with schwannoma and nine patients with meningioma who underwent APT-CEST MRI from November 2020 to April 2022 pre-operation. All 18 tumors were histologically diagnosed. There was a significant difference in magnetization transfer ratio asymmetry (MTRasym) values (0.033 ± 0.012 vs. 0.021 ± 0.004; p = 0.007) between the schwannoma and meningioma groups. Receiver operative curve analysis showed that MTRasym values clearly differentiated between the schwannoma and meningioma groups. At an MTRasym value threshold of 0.024, the diagnostic sensitivity, specificity, positive predictive value, and negative predictive values for MTRasym were 88.9%, 77.8%, 80.0%, and 87.5%, respectively. Our results demonstrated the ability of MTRasym values on APT-CEST imaging to discriminate patients with schwannomas from patients with meningiomas.
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Gokus, Tobias. "Nano-FTIR Correlation Nanoscopy for Organic and Inorganic Material Analysis." ECS Meeting Abstracts MA2022-01, no. 7 (July 7, 2022): 622. http://dx.doi.org/10.1149/ma2022-017622mtgabs.
Повний текст джерелаАнотація:
Significance: Correlation scanning probe techniques to complement nanoscale IR measurements for next generation sample characterization Scattering-type Scanning Near-field Optical Microscopy (s-SNOM) is a scanning probe approach to optical microscopy and spectroscopy, bypassing the ubiquitous diffraction limit of light to achieve a spatial resolution below 20 nanometers. s-SNOM employs the strong confinement of light at the apex of a sharp metallic atomic force microscopy (AFM) tip to create a nanoscale optical hot-spot. Analyzing the scattered light from the tip enables the extraction of the optical properties of the sample directly below the tip and yields nanoscale resolved images simultaneous to topography [1]. In addition, the technology has been advanced to enable Fourier-Transform Infrared Spectroscopy on the nanoscale (nano-FTIR) [2] using broadband radiation from the visible spectral range to THz frequencies. Recently, the combined analysis of complex nanoscale material systems by correlating near-field optical data with information obtained by other scanning probe microscopy (SPM)-based measurement methodologies has gained significant interest. For example, the material-characteristic nano-FTIR spectra of a phase-separated polystyrene/low-density polyethylene (PS/LDPE) polymer blend verifies sharp material interfaces by measuring a line profile across a ca. 1 μm sized LDPE island. Near-field reflection/absorption imaging at 1500cm-1 of the ca. 50nm thin film allows to selectively highlight the distribution of PS in the blend and simultaneously map the mechanical properties like adhesion of the different materials [3,4]. Further, we present results that correlate the near-field optical response of semiconducting samples like graphene (2D) or functional SRAM devices (3D) in different frequency ranges (mid-IR & THz) to Kelvin Probe Force Microscopy (KPFM) measurements. Thus, s-SNOM systems represent an ideal platform to gain novel insights into complex material systems by different near-field and AFM-based method. [1] F. Keilmann, R. Hillenbrand, Phil. Trans. R. Soc. Lond. A 362, 787 (2004). [2] F. Huth, et al., Nano Lett. 12, 3973 (2012). [3] B. Pollard, et al., Beilstein J. of Nanotechn. 7, 605 (2016). [4] I. Amenabar, et al., Nature Commun. 8, 14402 (2017).
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Carlesso, Chintha, Gorman, Samali, and Eriksson. "Effect of Kinase Inhibiting RNase Attenuator (KIRA) Compounds on the Formation of Face-to-Face Dimers of Inositol-Requiring Enzyme 1: Insights from Computational Modeling." International Journal of Molecular Sciences 20, no. 22 (November 6, 2019): 5538. http://dx.doi.org/10.3390/ijms20225538.
Повний текст джерелаАнотація:
Inositol-requiring enzyme 1α (IRE1α) is a transmembrane dual kinase/ribonuclease protein involved in propagation of the unfolded protein response (UPR). Inositol-requiring enzyme 1α is currently being explored as a potential drug target due to the growing evidence of its role in variety of disease conditions. Upon activation, IRE1 cleaves X-box binding protein 1 (XBP1) mRNA through its RNase domain. Small molecules targeting the kinase site are known to either increase or decrease RNase activity, but the allosteric relationship between the kinase and RNase domains of IRE1α is poorly understood. Subsets of IRE1 kinase inhibitors (known as “KIRA” compounds) bind to the ATP-binding site and allosterically impede the RNase activity. The KIRA compounds are able to regulate the RNase activity by stabilizing the monomeric form of IRE1α. In the present work, computational analysis, protein–protein and protein–ligand docking studies, and molecular dynamics simulations were applied to different IRE1 dimer systems to provide structural insights into the perturbation of IRE1 dimers by small molecules kinase inhibitors that regulate the RNase activity. By analyzing structural deviations, energetic components, and the number of hydrogen bonds in the interface region, we propose that the KIRA inhibitors act at an early stage of IRE1 activation by interfering with IRE1 face-to-face dimer formation thus disabling the activation of the RNase domain. This work sheds light on the mechanism of action of KIRA compounds and may assist in development of further compounds in, for example, cancer therapeutics. The work also provides information on the sequence of events and protein–protein interactions initiating the unfolded protein response.
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Vyse, Stuart A., Ulrike Herzschuh, Gregor Pfalz, Lyudmila A. Pestryakova, Bernhard Diekmann, Norbert Nowaczyk, and Boris K. Biskaborn. "Sediment and carbon accumulation in a glacial lake in Chukotka (Arctic Siberia) during the Late Pleistocene and Holocene: combining hydroacoustic profiling and down-core analyses." Biogeosciences 18, no. 16 (August 24, 2021): 4791–816. http://dx.doi.org/10.5194/bg-18-4791-2021.
Повний текст джерелаАнотація:
Abstract. Lakes act as important sinks for inorganic and organic sediment components. However, investigations of sedimentary carbon budgets within glacial lakes are currently absent from Arctic Siberia. The aim of this paper is to provide the first reconstruction of accumulation rates, sediment and carbon budgets from a lacustrine sediment core from Lake Rauchuagytgyn, Chukotka (Arctic Siberia). We combined multiple sediment biogeochemical and sedimentological parameters from a radiocarbon-dated 6.5 m sediment core with lake basin hydroacoustic data to derive sediment stratigraphy, sediment volumes and infill budgets. Our results distinguished three principal sediment and carbon accumulation regimes that could be identified across all measured environmental proxies including early Marine Isotope Stage 2 (MIS2) (ca. 29–23.4 ka cal BP), mid-MIS2–early MIS1 (ca. 23.4–11.69 ka cal BP) and the Holocene (ca. 11.69–present). Estimated organic carbon accumulation rates (OCARs) were higher within Holocene sediments (average 3.53 g OC m−2 a−1) than Pleistocene sediments (average 1.08 g OC m−2 a−1) and are similar to those calculated for boreal lakes from Quebec and Finland and Lake Baikal but significantly lower than Siberian thermokarst lakes and Alberta glacial lakes. Using a bootstrapping approach, we estimated the total organic carbon pool to be 0.26 ± 0.02 Mt and a total sediment pool of 25.7 ± 1.71 Mt within a hydroacoustically derived sediment volume of ca. 32 990 557 m3. The total organic carbon pool is substantially smaller than Alaskan yedoma, thermokarst lake sediments and Alberta glacial lakes but shares similarities with Finnish boreal lakes. Temporal variability in sediment and carbon accumulation dynamics at Lake Rauchuagytgyn is controlled predominantly by palaeoclimate variation that regulates lake ice-cover dynamics and catchment glacial, fluvial and permafrost processes through time. These processes, in turn, affect catchment and within-lake primary productivity as well as catchment soil development. Spatial differences compared to other lake systems at a trans-regional scale likely relate to the high-latitude, mountainous location of Lake Rauchuagytgyn.
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Grigale-Soročina, Zane, Ingmārs Birks, and Mārtiņš Kalniņš. "Analysis of Pigment Process and Production Techniques for Use in Nail Polish Systems." Solid State Phenomena 267 (October 2017): 7–11. http://dx.doi.org/10.4028/www.scientific.net/ssp.267.7.
Повний текст джерелаАнотація:
This report explores two key manufacturing processes, and assesses multiple parameters for optimization of these processes. High shear dispersion using a rotor-stator style homogenizer and high-energy grinding using a basket type mill with zirconium balls as the grinding media were studied. The variation between grinding organic and inorganic pigments was assessed as they give different challenges and require different operations to produce successful results. Obtaining the correct particle size distribution of pigments for decorative coatings is of paramount importance for achieving a high quality, functioning, aesthetically pleasing finished product. This analysis will explore the de-agglomeration and dispersion of inorganic TiO2 and organic Red 6 pigments using basket milling technology. High energy basket mill grinding produced the smallest particle size and smallest particle size distribution comparing with high shear dispersion. Used technology allowed to achieve inorganic pigment TiO2 particle size and particle size distribution suitable for use in coatings on natural nails. For organic pigment Red 6 used technology should be customized.
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Ledenev, A., S. Kozodaev, V. Percev, E. Baranov, T. Zagoruyko, and D. Vnukov. "MECHANISMS OF ACT OF VARIOUS KINDS OF ORGANIC MINERAL ADDITIVES IN CEMENT SYSTEM." Bulletin of Belgorod State Technological University named after. V. G. Shukhov 6, no. 9 (September 13, 2021): 8–19. http://dx.doi.org/10.34031/2071-7318-2021-6-9-8-19.
Повний текст джерелаАнотація:
The article presents the direction of development of the scientific and practical foundations of the technology of cement concretes associated with the use of organic mineral additives. It allows to improve the physical and mechanical characteristics of products and structures. The purpose of research is determination of the mechanism of act organic-mineral additives including reductants of the various physical and chemical nature and an experimental estimation of their effect on a mineral phase structure and properties of cement system. Organic mineral additives are applied in studies for which new options of components of the various physical and chemical nature are observed and received. The study of possible mechanisms of effect organic mineral additives conducted on a water-cement paste and on concreting cement system with roent-gen-phase analysis application are made. The effect of organic mineral additives on properties watering out and concreting cement systems is established. The effect of organic mineral additives including reduct-ants of the various physical and chemical nature, on a modification of a mineral phase structure of growths in the course of a hydration solidification and set strength is determined. The data obtained allow to broaden the understanding of organic-mineral additives effect on a structure and properties of cement sys-tem and to suggest accessible raw reductants in new effective organic mineral additives for obtaining of cement concretes with improved physical-mechanical performances.
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Coppola, Canaan, Brooks Hopkins, Steven Huhn, Zhimei Du, Zuyi Huang, and William J. Kelly. "Investigation of the Impact from IL-2, IL-7, and IL-15 on the Growth and Signaling of Activated CD4+ T Cells." International Journal of Molecular Sciences 21, no. 21 (October 22, 2020): 7814. http://dx.doi.org/10.3390/ijms21217814.
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While CAR-T therapy is a growing and promising area of cancer research, it is limited by high cost and the difficulty of consistently culturing T-cells to therapeutically relevant concentrations ex-vivo. Cytokines IL-2, IL-7 and IL-15 have been found to stimulate the growth of T cells, however, the optimized combination of these three cytokines for T cell proliferation is unknown. In this study, we designed an integrated experimental and modeling approach to optimize cytokine supplementation for rapid expansion in clinical applications. We assessed the growth data for statistical improvements over no cytokine supplementation and used a systems biology approach to identify genes with the highest magnitude of expression change from control at several time points. Further, we developed a predictive mathematical model to project the growth rate for various cytokine combinations, and investigate genes and reactions regulated by cytokines in activated CD4+ T cells. The most favorable conditions from the T cell growth study and from the predictive model align to include the full range of IL-2 and IL-7 studied, and at lower levels of IL-15 (6 ng/mL or 36 ng/mL). The highest growth rates were observed where either IL-2 or IL-7 was at the highest concentration tested (15 ng/mL for IL-2 and 80 ng/mL for IL-7) while the other was at the lowest (1 ng/mL for IL-2 and 6 ng/mL for IL-7), or where both IL-2 and IL-7 concentrations are moderate-corresponding to condition keys 200, 020, and 110 respectively. This suggests a synergistic interaction of IL-2 and IL-7 with regards to promoting optimal proliferation and survival of the activated CD4+ T cells. Transcriptomic data analysis identified the genes and transcriptional regulators up/down-regulated by each of the cytokines IL-2, IL-7, and IL-15. It was found that the genes with persistent expressing changes were associated with major pathways involved in cell growth and proliferation. In addition to influencing T cell metabolism, the three cytokines were found to regulate specific genes involved in TCR, JAK/STAT, MAPK, AKT and PI3K-AKT signaling. The developed Fuzzy model that can predict the growth rate of activated CD4+ T cells for various combinations of cytokines, along with identified optimal cytokine cocktails and important genes found in transcriptomic data, can pave the way for optimizing activated CD4 T cells by regulating cytokines in the clinical setting.
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KURAMOTO, Nobuhiro. "Application of Raman Spectroscopy to Nondestructive Analysis of Organic and Inorganic Pigments in Paint Systems." Journal of the Japan Society of Colour Material 74, no. 8 (2001): 391–94. http://dx.doi.org/10.4011/shikizai1937.74.391.
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Leitner, Simon, Wendelin Feichtinger, Stefan Mayer, Florian Mayer, Dustin Krompetz, Rebecca Hood-Nowotny, and Andrea Watzinger. "UAV-based sampling systems to analyse greenhouse gases and volatile organic compounds encompassing compound-specific stable isotope analysis." Atmospheric Measurement Techniques 16, no. 2 (January 27, 2023): 513–27. http://dx.doi.org/10.5194/amt-16-513-2023.
Повний текст джерелаАнотація:
Abstract. The study herein reports on the development and testing of sampling systems (and subsequent analytical setups) that were deployed on an unmanned aerial vehicle (UAV) for the purpose of analysing greenhouse gases (GHGs) and volatile organic compounds (VOCs) in the lower atmospheric boundary layer. Two sampling devices, both of which can be mounted to an UAV with a payload capability greater than 1 kg, were tested for respective sampling and analysis of specific GHGs (carbon dioxide, CO2, and methane, CH4) and VOCs (chlorinated ethenes, CEs). The gas analyses included measurements of the molar amounts and the respective stable carbon isotope ratios. In addition to compound calibration in the laboratory, the functionality of the samplers and the UAV-based sampling was tested in the field. Atmospheric air was either flushed through sorbent tubes for VOC sampling or collected and sampled in glass vials for GHG analysis. The measurement setup for the sorbent tubes achieved analyte mass recovery rates of 63 %–100 % (more favourable for lower chlorinated ethenes), when prepared from gaseous or liquid calibration standards, and reached a precision (2σ) better than 0.7 ‰ for δ13C values in the range of 0.35–4.45 nmol. The UAV-equipped samplers were tested over two field sampling campaigns designed to (1) compare manual and UAV-collected samples taken up a vertical profile at a forest site and (2) identify potential emissions of CO2, CH4 or VOC from a former domestic waste dump. The precision of CO2 measurements from whole air samples was ≤7.3 µmol mol−1 and ≤0.3 ‰ for δ13C values and ≤0.03 µmol mol−1 and ≤0.2 ‰ for CH4 working gas standards. The results of the whole air sample analyses for CO2 and CH4 were sufficiently accurate to detect and localise potential landfill gas emissions from a secured former domestic waste dump using level flight. Vertical CO2 profiles from a forest location showed a causally comprehensive pattern in the molar ratios and stable carbon isotope ratios but also the potential falsification of the positional accuracy of a UAV-assisted air sample due to the influence of the rotor downwash. The results demonstrate that the UAV sampling systems presented here represent a viable tool for atmospheric background monitoring, as well as for evaluating and identifying emission sources. By expanding the part of the lower atmosphere that can be practicably sampled over horizontal and vertical axes, the presented UAV-capable sampling systems, which also allow for compound-specific stable isotope analysis (CSIA), may facilitate an improved understanding of surface–atmosphere fluxes of trace gas.
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Ogunmola, Omotoso, Christiana Afolabi, Charles Adesina, and Kelechi Ilechukwu. "A comparative analysis of the profitability and technical efficiency of vegetable production under two farming systems in Nigeria." Journal of Agricultural Sciences, Belgrade 66, no. 1 (2021): 87–104. http://dx.doi.org/10.2298/jas2101087o.
Повний текст джерелаАнотація:
Increasing agricultural productivity enhanced by versatile production systems is critical for sustainable food security and economic development. The study aims to compare the profitability and technical efficiency of vegetable production and factors influencing the technical efficiency of vegetable production between inorganic and organic farming systems in Imo State, Nigeria. Primary data were collected using structured questionnaires comprising 100 vegetable farmers using a multistage sampling procedure. The budgetary analysis and stochastic production frontier model were used to estimate the profitability and the technical efficiencies of the enterprise. An average farmer realized N277,445.24 and N190,506.04 per hectare as profit from inorganic and organic vegetable production and can potentially earn N4.40 and N2.89 on every Naira invested, respectively. However, the inorganic farming system achieved significantly higher returns than the organic farming system. The mean technical efficiencies for organic and inorganic vegetable farmers were 89.57% and 75.64%, respectively. Farm size, labour and the quantity of seeds were the crucial factors that affected the technical efficiency under both farming systems. Also, age, years of education and farming experience were the significant variables that influenced the technical inefficiency of inorganic farmers, whereas years of education and household size significantly influenced the technical inefficiency of organic farmers. This study advocates for subsidized inputs for organic farmers to compensate for their lower yields and policies that would attract young people to vegetable farming to increase the production level.
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Zavotka, Susan. "Entertainment and Communications Technology in the Home: A Historic Analysis of Organic and Inorganic Design Systems." Journal of Interior Design 16, no. 1 (May 1990): 29–36. http://dx.doi.org/10.1111/j.1939-1668.1990.tb00027.x.
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Hägele, Gerhard. "Application of Efficient Program Systems for Spectral Analysis of Phosphorus Containing Compounds." Phosphorus, Sulfur, and Silicon and the Related Elements 144, no. 1 (January 1, 1999): 659–61. http://dx.doi.org/10.1080/10426509908546331.
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Bakaj, M., and M. Zimmer. "Conformational analysis of copper(II) 1,4,8,11-tetraazacyclotetradecane macrocyclic systems." Journal of Molecular Structure 508, no. 1-3 (September 1999): 59–72. http://dx.doi.org/10.1016/s0022-2860(99)00032-0.
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Zhao, Yue, Michelle C. Fairhurst, Lisa M. Wingen, Véronique Perraud, Michael J. Ezell, and Barbara J. Finlayson-Pitts. "New insights into atmospherically relevant reaction systems using direct analysis in real-time mass spectrometry (DART-MS)." Atmospheric Measurement Techniques 10, no. 4 (April 11, 2017): 1373–86. http://dx.doi.org/10.5194/amt-10-1373-2017.
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Abstract. The application of direct analysis in real-time mass spectrometry (DART-MS), which is finding increasing use in atmospheric chemistry, to two different laboratory model systems for airborne particles is investigated: (1) submicron C3–C7 dicarboxylic acid (diacid) particles reacted with gas-phase trimethylamine (TMA) or butylamine (BA) and (2) secondary organic aerosol (SOA) particles from the ozonolysis of α-cedrene. The diacid particles exhibit a clear odd–even pattern in their chemical reactivity toward TMA and BA, with the odd-carbon diacid particles being substantially more reactive than even ones. The ratio of base to diacid in reacted particles, determined using known diacid–base mixtures, was compared to that measured by high-resolution time-of-flight aerosol mass spectrometry (HR-ToF-AMS), which vaporizes the whole particle. Results show that DART-MS probes ∼ 30 nm of the surface layer, consistent with other studies on different systems. For α-cedrene SOA particles, it is shown that varying the temperature of the particle stream as it enters the DART-MS ionization region can distinguish between specific components with the same molecular mass but different vapor pressures. These results demonstrate the utility of DART-MS for (1) examining reactivity of heterogeneous model systems for atmospheric particles and (2) probing components of SOA particles based on volatility.
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Acharya, Mohan, Amanda J. Ashworth, Yichao Yang, Joan M. Burke, Jung Ae Lee, and Roshani Sharma Acharya. "Soil microbial diversity in organic and non-organic pasture systems." PeerJ 9 (April 22, 2021): e11184. http://dx.doi.org/10.7717/peerj.11184.
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Understanding the effects of organic pasture management on the soil microbiome is important for sustainable forage production since soil microbiome diversity contributes to improved nutrient cycling, soil structure, plant growth, and environmental resiliency; however, the soil microbiome response to pasture management is largely unknown. This study assessed the soil microbial diversity, richness, and community structure following 10 years of pasture management (organic or non-organic) of the V4 region of the 16S rRNA using the Illumina MiSeq platform. Soil samples were collected from 0–15 cm in July and August from 2017–2018 and soil nutrient properties (nutrients, carbon, nitrogen, and pH) quantified and correlated with soil microbial diversity. Overall, greater soil bacterial species richness (P ≤ 0.05) occurred in organic relative to non-organic (conventional) systems. Management affected bacterial species richness (Chao1), with greater richness occurring in organic pasture soils and less richness occurring in non-organic systems (P ≤ 0.05). Similarly, management affected bacterial evenness (Simpson’s index), with a more diverse community occurring in organically managed soils relative to non-organic pastures (P ≤ 0.05). Linear discriminant analysis effect size analysis showed statistically significant and biologically consistent differences in bacterial taxa in organic compared with non-organic soils. Therefore, there was a shift in bacterial community structure in organic relative to non-organic soils (P ≤ 0.05). Additionally, soil nutrients (Fe, Mg, Ni, S, Al, K, Cd, and Cu), pH, C, and N were correlated with one or more dominant bacterial phyla (Gemmatimonadetes, Planctomycetes, Firmicutes, Chloroflexi, Actinobacteria, and Acidobacteria). Overall, pasture management affected soil microbial diversity, with greater diversity occurring in organic than non-organic systems, likely owing to applications of organic poultry litter in organic systems compared to non-organic management (use of inorganic-fertilizers and herbicides). Results indicate that when pastures are converted to organic production systems, soil microbial richness and diversity may increase, thereby resulting in enhanced soil microbiome diversity and overall ecosystem services.
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Helmers, Glenn A., Michael R. Langemeier, and Joseph Atwood. "An economic analysis of alternative cropping systems for east-central Nebraska." American Journal of Alternative Agriculture 1, no. 4 (1986): 153–58. http://dx.doi.org/10.1017/s0889189300001223.
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AbstractThirteen cropping systems were analyzed with respect to profitability and risk for east-central Nebraska. The systems were developed from 1) a four-year rotation containing a small grain, 2) two row crop rotations, 3) three continuous cropped alternatives, and 4) combinations of continuous cropped alternatives. Three systems were developed from the four-year rotation including two alternative treatments of inorganic chemicals as well as an organic alternative. Eight years of experimental yields, historical prices, and estimated costs were combined to estimate net returns for each of the thirteen systems. Risk was analyzed as net return variability using statistical characteristics of the net return series. The stability component of rotation risk was separated from the diversification component. We found rotations to have higher average net returns than continuously cropped systems. Different chemical treatments (including organic) had little impact on profitability. Rotations had lower return variability than most continuous crops. The organic treatment did not decrease variability of returns compared to other chemical systems.
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Waiman, Carolina V., Ignacio E. dell’Erba, Carlos A. Chesta, and María Lorena Gómez. "Hybrid Films Based on a Bridged Silsesquioxane Doped with Goethite and Montmorillonite Nanoparticles as Sorbents of Wastewater Contaminants." Journal of Nanomaterials 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/6286247.
Повний текст джерелаАнотація:
The synthesis and characterization of silsesquioxane (SSO) films with pendant dodecyl groups and doped with goethite (Gt) or montmorillonite (MMT) nanoparticles were carried out and the new materials tested as sorbents of diverse contaminants. The synthetic method used yielded SSO films with the inorganic substrates homogeneously distributed within the polymeric matrices. The new materials were characterized by SEM, FTIR, XRD, and DSC and tested to evaluate their capability for adsorbing metallic cations, organic dyes, and phosphate, frequent contaminants of industrial effluents. All films were found suitable for removing metallic cations. Results also showed that the SSO films undoped and doped with Gt are primarily apt for anionic compounds removal. Although the SSO films doped with MMT are capable of removing cationic contaminants from aqueous samples, the stiffness of the SSO matrix hinders MMT properties as an adsorbent. The possibility of dispersing nanoparticulate systems in the stable and chemically inert SSO matrices simplifies their application for contaminant removal, particularly because it makes the separation process of the absorbed pollutant from the treated medium easier.
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Sanmukh, Swapnil, Krishna Khairnar, Waman Paunikar, and Satish Lokhande. "Understanding carbon regulation in aquatic systems - Bacteriophages as a model." F1000Research 4 (June 1, 2015): 138. http://dx.doi.org/10.12688/f1000research.6031.1.
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The bacteria and their phages are the most abundant constituents of the aquatic environment, and so represent an ideal model for studying carbon regulation in an aquatic system. The microbe-mediated interconversion of bioavailable organic carbon (OC) into dissolved organic carbon (DOC) by the microbial carbon pump (MCP) has been suggested to have the potential to revolutionize our view of carbon sequestration. It is estimated that DOC is the largest pool of organic matter in the ocean and, though a major component of the global carbon cycle, its source is not yet well understood. A key element of the carbon cycle is the microbial conversion of DOC into inedible forms. The primary aim of this study is to understand the phage conversion from organic to inorganic carbon during phage-host interactions.Time studies of phage-host interactions under controlled conditions reveal their impact on the total carbon content of the samples and their interconversion of organic and inorganic carbon compared to control samples. A total organic carbon (TOC) analysis showed an increase in inorganic carbon content by 15-25 percent in samples with bacteria and phage compared to samples with bacteria alone. Compared to control samples, the increase in inorganic carbon content was 60-70-fold in samples with bacteria and phage, and 50-55-fold for samples with bacteria alone. This study indicates the potential impact of phages in regulating the carbon cycle of aquatic systems.
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Zeng, Linghan, Amy P. Sullivan, Rebecca A. Washenfelder, Jack Dibb, Eric Scheuer, Teresa L. Campos, Joseph M. Katich, Ezra Levin, Michael A. Robinson, and Rodney J. Weber. "Assessment of online water-soluble brown carbon measuring systems for aircraft sampling." Atmospheric Measurement Techniques 14, no. 10 (October 1, 2021): 6357–78. http://dx.doi.org/10.5194/amt-14-6357-2021.
Повний текст джерелаАнотація:
Abstract. Brown carbon (BrC) consists of particulate organic species that preferentially absorb light at visible and ultraviolet wavelengths. Ambient studies show that as a component of aerosol particles, BrC affects photochemical reaction rates and regional to global climate. Some organic chromophores are especially toxic, linking BrC to adverse health effects. The lack of direct measurements of BrC has limited our understanding of its prevalence, sources, evolution, and impacts. We describe the first direct, online measurements of water-soluble BrC on research aircraft by three separate instruments. Each instrument measured light absorption over a broad wavelength range using a liquid waveguide capillary cell (LWCC) and grating spectrometer, with particles collected into water by a particle-into-liquid sampler (CSU PILS-LWCC and NOAA PILS-LWCC) or a mist chamber (MC-LWCC). The instruments were deployed on the NSF C-130 aircraft during WE-CAN 2018 as well as the NASA DC-8 and the NOAA Twin Otter aircraft during FIREX-AQ 2019, where they sampled fresh and moderately aged wildfire plumes. Here, we describe the instruments, calibrations, data analysis and corrections for baseline drift and hysteresis. Detection limits (3σ) at 365 nm were 1.53 Mm−1 (MC-LWCC; 2.5 min sampling time), 0.89 Mm−1 (CSU PILS-LWCC; 30 s sampling time), and 0.03 Mm−1 (NOAA PILS-LWCC; 30 s sampling time). Measurement uncertainties were 28 % (MC-LWCC), 12 % (CSU PILS-LWCC), and 11 % (NOAA PILS-LWCC). The MC-LWCC system agreed well with offline measurements from filter samples, with a slope of 0.91 and R2=0.89. Overall, these instruments provide soluble BrC measurements with specificity and geographical coverage that is unavailable by other methods, but their sensitivity and time resolution can be challenging for aircraft studies where large and rapid changes in BrC concentrations may be encountered.
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Roussos, Peter Anargyrou, Athanassios Triantafillidis, Evaggelos Kepolas, Panagiotis Peppas, Anastassia Piou, Maria Zoti, and Dionisios Gasparatos. "Effects of Integrated and Organic Management on Strawberry (cv. Camarosa) Plant Growth, Nutrition, Fruit Yield, Quality, Nutraceutical Characteristics, and Soil Fertility Status." Horticulturae 8, no. 2 (February 21, 2022): 184. http://dx.doi.org/10.3390/horticulturae8020184.
Повний текст джерелаАнотація:
Strawberry plants (cv. Camarosa) were cultivated under organic (ORG) and integrated management (INT) practices to assess possible differences in plant growth, fruit production and quality, soil fertility, and plant nutrition. Two integrated fertilizer schemes were used, differentiating based on the origin of the major organic fertilizer used, i.e., chicken manure (I-ACT) versus fermented fungal biomass (I-AGR). Plant growth was higher under I-ACT treatment, whereas leaf area did not differ among treatments. The two integrated schemes resulted in higher fruit production. The organoleptic characteristics of the fruits did not differ, and this was also confirmed by a taste panel. The antioxidant capacity and flavanol concentration of organically produced fruits were higher, as well as fruit carbohydrate and total organic acid concentration. Leaf nitrogen concentration was higher under I-ACT, followed by ORG. I-ACT resulted in high leaf potassium concentration, as well as iron and manganese (together with I-AGR). Under ORG management, soil EC was found to be lower, whereas soil available phosphorus concentration was highest. Discriminant analysis taking into account fruit quality and phytochemical properties distinguished ORG management from the two integrated management practices, and when soil properties and leaf nutrient concentration were considered; all three systems were separated, indicating that the type of fertilizers used plays a significant role.
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Wang, Dong-Yan, Xue-Li Hou, and Xue-Nan Li. "Isostructural organic–inorganic hybrid compounds: triethylcholine tribromidocadmate and triethylcholine tribromidomercurate." Acta Crystallographica Section C Structural Chemistry 71, no. 8 (July 25, 2015): 728–32. http://dx.doi.org/10.1107/s2053229615013303.
Повний текст джерелаАнотація:
In order to search for new anionic architectures and develop useful organic–inorganic hybrid materials in halometallate systems, two new crystalline organic–inorganic hybrid compounds have been prepared,i.e. catena-poly[triethyl(2-hydroxyethyl)azanium [[bromidocadmate(II)]-di-μ-bromido]], {(C8H20NO)[CdBr3]}n, (1), andcatena-poly[triethyl(2-hydroxyethyl)azanium [[bromidomercurate(II)]-di-μ-bromido]], {(C8H20NO)[HgBr3]}n, (2), and the structures determined by X-ray diffraction analysis. The compounds are isostructural, crystallizing in the space groupP21/n. The metal centres are five-coordinated by bromide anions, giving a slightly distorted trigonal–bipyramidal geometry. The crystal structures consist of one-dimensional edge-sharing chains ofMBr5trigonal bipyramids, between which triethylcholine counter-cations are intercalated. O—H...Br hydrogen-bonding interactions are present between the cations and anions.
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Lerner, Brian M., Jessica B. Gilman, Kenneth C. Aikin, Elliot L. Atlas, Paul D. Goldan, Martin Graus, Roger Hendershot, et al. "An improved, automated whole air sampler and gas chromatography mass spectrometry analysis system for volatile organic compounds in the atmosphere." Atmospheric Measurement Techniques 10, no. 1 (January 26, 2017): 291–313. http://dx.doi.org/10.5194/amt-10-291-2017.
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Abstract. Volatile organic compounds were quantified during two aircraft-based field campaigns using highly automated, whole air samplers with expedited post-flight analysis via a new custom-built, field-deployable gas chromatography–mass spectrometry instrument. During flight, air samples were pressurized with a stainless steel bellows compressor into electropolished stainless steel canisters. The air samples were analyzed using a novel gas chromatograph system designed specifically for field use which eliminates the need for liquid nitrogen. Instead, a Stirling cooler is used for cryogenic sample pre-concentration at temperatures as low as −165 °C. The analysis system was fully automated on a 20 min cycle to allow for unattended processing of an entire flight of 72 sample canisters within 30 h, thereby reducing typical sample residence times in the canisters to less than 3 days. The new analytical system is capable of quantifying a wide suite of C2 to C10 organic compounds at part-per-trillion sensitivity. This paper describes the sampling and analysis systems, along with the data analysis procedures which include a new peak-fitting software package for rapid chromatographic data reduction. Instrument sensitivities, uncertainties and system artifacts are presented for 35 trace gas species in canister samples. Comparisons of reported mixing ratios from each field campaign with measurements from other instruments are also presented.