Статті в журналах: "Analyses en cellules uniques"

1

Lacoux, C., M. Truchi, J. Fassy, I. Manosalva-Pena, M. Gautier, V. Magnone, K. Lebrigand, et al. "Analyse des longs ARN non codants régulés par l’hypoxie dans les cellules d’adénocarcinome pulmonaire à l’aide d’un crible basé sur l’interférence CRISPR sur cellules uniques." Revue des Maladies Respiratoires 40, no. 2 (February 2023): 122–23. http://dx.doi.org/10.1016/j.rmr.2022.11.029.

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2

Dariane, Charles, Manon Baures, Julien Anract, Nicolas Barry Delongchamps, Jacques-Emmanuel Guidotti, and Vincent Goffin. "Progéniteurs luminaux prostatiques." médecine/sciences 39, no. 5 (May 2023): 429–36. http://dx.doi.org/10.1051/medsci/2023058.

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Les traitements médicaux de l’hyperplasie bénigne et du cancer de la prostate reposent essentiellement sur l’inhibition de la signalisation androgénique. Bien qu’initialement efficaces, ces traitements sont tôt ou tard confrontés à une résistance thérapeutique. Des données récentes de séquençage d’ARN sur cellules uniques montrent que les cellules luminales survivant à la déprivation androgénique dans ces contextes pathologiques présentent un profil moléculaire semblable à celui de cellules luminales progénitrices, présentes en faible quantité dans un contexte physiologique. Ce profil moléculaire pourrait constituer un hub de résistance à la castration et résulter, en partie, de la reprogrammation des cellules luminales tumorales. L’inhibition thérapeutique de cette plasticité cellulaire constitue une piste prometteuse pour limiter la progression du cancer prostatique.

3

Isaac, Juliane, Mélodie M. Clerc, François C. Ferré, and Benjamin P. J. Fournier. "Les cellules mésenchymateuses orales, une niche spécifique, du développement à la régénération." médecine/sciences 40, no. 1 (January 2024): 24–29. http://dx.doi.org/10.1051/medsci/2023191.

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Les tissus muqueux et osseux oraux présentent des propriétés uniques. Les fibroblastes de la muqueuse orale et les ostéoblastes des mâchoires, issus des crêtes neurales crâniennes, jouent un rôle clé dans la cicatrisation/réparation. Ces cellules expriment un répertoire spécifique de gènes associés à leurs propriétés régénératives, mais aussi liés aux maladies rares crâniofaciales. La connaissance de ces tissus ouvre des perspectives cliniques pour la régénération tissulaire et la réparation des défauts osseux et muqueux. Ces avancées multidisciplinaires ont aussi un impact prometteur sur la prise en charge des maladies liées au parodonte et sur l’amélioration de la santé bucco-dentaire.

4

Remacle, Françoise, and Raphael D. Levine. "Prédiction de la réponse moléculaire à des perturbations mesurée sur des cellules uniques." médecine/sciences 30, no. 12 (December 2014): 1129–35. http://dx.doi.org/10.1051/medsci/20143012016.

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5

Lardenois, A., B. Evrard, A. Suglia, S. Léonard, L. Lesné, I. Coiffec, B. Jégou, S. Mazaud-Guittot, F. Chalmel, and A. D. Rolland. "Nouveaux acteurs de la différenciation gonadique normale et pathologique, approches cellules uniques chez l’Homme." Annales d'Endocrinologie 82, no. 5 (October 2021): 225. http://dx.doi.org/10.1016/j.ando.2021.07.020.

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Grandjean-Closson, Eva, Camille Heckmann, Corentin Le Coz, Isaline Louvet, Matthieu Neri, and Corine Bertolotto. "L’analyse des mélanomes uvéaux primaires à l’aide de la technique de séquençage d’ARN de cellules uniques." médecine/sciences 38, no. 8-9 (August 2022): 737–39. http://dx.doi.org/10.1051/medsci/2022113.

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7

Achille, Tadaha Moffo. "Perception De L’échec Et Motivation Académique Des Etudiants En 2e Année En Faculté De Lettres Et Des Sciences Humaines A l’Université De Douala." Journal of Advanced Psychology 5, no. 2 (March 17, 2024): 16–32. http://dx.doi.org/10.47941/japsy.1730.

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But : L’examen de l’influence de la perception de l’échec sur la motivation scolaire des étudiants est l’objectif que se donne cet article. Méthodologie : Pour atteindre celui-ci, les données de l’enquête de terrain auprès de 84 étudiants issus de la deuxième année en Faculté de Lettres et des Sciences Humaines (FLSH) à l’Université de Douala en situation d’échec sont soumises à des analyses descriptives et inférentielles. Résultats : Les résultats du rhô de Spearman obtenus de l’analyse du questionnaire d’autoévaluation et du « motivomètre » relèvent que le niveau de motivation scolaire est très bas chez ces étudiants et cela semble dû à une mauvaise perception qu’ils ont construite autour de leur échec précédent. En effet, les analyses ont montré un effet positif et significatif des représentations sociales de l’échec, des clichés du nombre d’échec et des croyances autour de l’échec sur la motivation scolaire chez ces étudiants. Dès lors, il semble nécessaire pour l’Etat et les Universités en place de décider de rendre l’accès de ces étudiants plus facile aux cellules d’écoute et d’accompagnement psychopédagogique. Contributeur Unique A La Théorie Politiques, Aux Et A La Pratique : Ceci par les moyens de la sensibilisation permanente et médiatisée sur l’importance de rencontrer régulièrement un conseiller d’orientation et/ou un psychologue scolaire. Et aussi la nécessité d’impliquer les familles afin d’influencer les cognitions sociales qui entourent l’échec scolaire et par la même occasion de réduire la déperdition scolaire.

8

Aggoun, Samir. "Complementary investigations in a histology-embryology lab." Batna Journal of Medical Sciences (BJMS) 2, no. 2 (December 30, 2012): 182–85. http://dx.doi.org/10.48087/bjmstf.2015.2218.

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L’activité d’un laboratoire d’histologie–embryologie repose sur deux domaines qui s’occupent de l’exploration des cellules. Le premier s'intéresse aux cellules haploïdes ou spermatozoïdes : c’est la biologie de la reproduction, qui est une discipline médicale qui réunit toutes les analyses biologiques du sperme humain initiées par le spermogramme et accomplies par les explorations de la procréation médicalement assistée ; ces analyses sont réalisées dans le cadre du bilan d'infertilité masculine. Le second domaine s’occupe des cellules diploïdes : c’est la cytologie ou cytopathologie, qui s'appuie principalement sur l'observation microscopique des altérations morphologiques des cellules recueillies d’organes ou des liquides biologiques.

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Zurawski, Jeffrey V., Jonathan M. Conway, Laura L. Lee, Hunter J. Simpson, Javier A. Izquierdo, Sara Blumer-Schuette, Intawat Nookaew, Michael W. W. Adams, and Robert M. Kelly. "Comparative Analysis of Extremely Thermophilic Caldicellulosiruptor Species Reveals Common and Unique Cellular Strategies for Plant Biomass Utilization." Applied and Environmental Microbiology 81, no. 20 (August 7, 2015): 7159–70. http://dx.doi.org/10.1128/aem.01622-15.

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ABSTRACTMicrobiological, genomic and transcriptomic analyses were used to examine three species from the bacterial genusCaldicellulosiruptorwith respect to their capacity to convert the carbohydrate content of lignocellulosic biomass at 70°C to simple sugars, acetate, lactate, CO2, and H2.Caldicellulosiruptor bescii,C. kronotskyensis, andC. saccharolyticussolubilized 38%, 36%, and 29% (by weight) of unpretreated switchgrass (Panicum virgatum) (5 g/liter), respectively, which was about half of the amount of crystalline cellulose (Avicel; 5 g/liter) that was solubilized under the same conditions. The lower yields withC. saccharolyticus, not appreciably greater than the thermal control for switchgrass, were unexpected, given that its genome encodes the same glycoside hydrolase 9 (GH9)-GH48 multidomain cellulase (CelA) found in the other two species. However, the genome ofC. saccharolyticuslacks two other cellulases with GH48 domains, which could be responsible for its lower levels of solubilization. Transcriptomes for growth of each species comparing cellulose to switchgrass showed that many carbohydrate ABC transporters and multidomain extracellular glycoside hydrolases were differentially regulated, reflecting the heterogeneity of lignocellulose. However, significant differences in transcription levels for conserved genes among the three species were noted, indicating unexpectedly diverse regulatory strategies for deconstruction for these closely related bacteria. Genes encoding the Che-type chemotaxis system and flagellum biosynthesis were upregulated inC. kronotskyensisandC. besciiduring growth on cellulose, implicating motility in substrate utilization. The results here show that capacity for plant biomass deconstruction varies acrossCaldicellulosiruptorspecies and depends in a complex way on GH genome inventory, substrate composition, and gene regulation.

10

Ghalloussi, H., J. Doyen, A. Leysalle, M. Poudenx, H. Bérard, N. Venissac, and P. Bondiau. "Étude de l’efficacité à 3ans du CyberKnife® dans les carcinomes bronchiques non à petites cellules de stade I uniques ou multiples chez 289 patients." Cancer/Radiothérapie 19, no. 6-7 (October 2015): 642. http://dx.doi.org/10.1016/j.canrad.2015.07.012.

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11

Ferry-Danini, Juliette. "La médecine narrative face à l’impossible singularité des récits." Lato Sensu: Revue de la Société de philosophie des sciences 7, no. 2 (March 12, 2020): 1–6. http://dx.doi.org/10.20416/lsrsps.v7i2.1.

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Selon l’une des thèses les plus répétées de la médecine narrative, la théorie littéraire, ou plus largement, la narration, permettrait aux membres du personnel médical d’appréhender les récits des patients et par là, de prendre en considération leurs expériences dans leur singularité absolue. Dans ma contribution, je soulignerai quelques limites de cette thèse. J’appuierai mon analyse sur un exemple de récit dominant de maladie, les récits portant sur le cancer du sein aux États-Unis au XXe siècle, à partir des analyses féministes qui en ont été faites. Ainsi je montrerai d’une part qu’il est peu plausible que les récits des patients soient absolument singuliers et uniques, en un sens pertinent, et d’autre part que certains récits peuvent devenir dominants et marginaliser des récits qui voudraient s’en démarquer.

12

Forterre, Patrick, and Morgan Gaïa. "Les virus et l’émergence des cellules eucaryotes modernes." médecine/sciences 38, no. 12 (December 2022): 990–98. http://dx.doi.org/10.1051/medsci/2022164.

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Il est maintenant bien établi que les virus ont joué un rôle important dans l’évolution des eucaryotes modernes. Dans cette revue, nous commentons le rôle qu’ils ont pu jouer dans l’eucaryogenèse. Nous discutons les analyses phylogénétiques qui mettent en évidence l’origine virale de plusieurs protéines clés de la biologie moléculaire des eucaryotes et des observations récentes qui, par analogie, pourraient suggérer une origine virale du noyau cellulaire. Nous mettons en parallèle la complexité des eucaryotes avec l’unicité de leur virosphère et avançons l’hypothèse selon laquelle des mécanismes de la différenciation cellulaire auraient leur source dans ceux mis en œuvre par les virus pour transformer les cellules infectées en cellules virales.

13

Eidinejad, Zahra, Reza Saadati, and Dušan D. Repovš. "Mittag-Leffler Stability and Attractiveness of Pseudo Almost Periodic Solutions for Delayed Cellular Neural Networks." Journal of Function Spaces 2022 (October 11, 2022): 1–15. http://dx.doi.org/10.1155/2022/3186963.

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We consider a class of nonautonomous cellular neural networks (CNNs) with mixed delays, to study the solutions of these systems which are type pseudo almost periodicity. Using general measure theory and the Mittag-Leffler function, we obtain the existence of unique solutions for cellular neural equations and investigate the Mittag-Leffler stability and attractiveness of pseudo almost periodic functions. We also present numerical examples to illustrate the application of our results.

14

Mba Medie, Felix, Iskandar Ben Salah, Michel Drancourt, and Bernard Henrissat. "Paradoxical conservation of a set of three cellulose-targeting genes in Mycobacterium tuberculosis complex organisms." Microbiology 156, no. 5 (May 1, 2010): 1468–75. http://dx.doi.org/10.1099/mic.0.037812-0.

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The genome of the tuberculosis agent Mycobacterium tuberculosis encodes a putative cellulose-binding protein (CBD2), one candidate cellulase (Cel12), and one fully active cellulase (Cel6). This observation is puzzling, because cellulose is a major component of plant cell walls, whereas M. tuberculosis is a human pathogen without known contact with plants. In order to investigate the biological role of such cellulose-targeting genes in M. tuberculosis we report here the search for and transcription analysis of this set of genes in the genus Mycobacterium. An in silico search for cellulose-targeting orthologues found that only 2.5 % of the sequenced bacterial genomes encode the Cel6, Cel12 and CBD2 gene set simultaneously, including those of the M. tuberculosis complex (MTC) members. PCR amplification and sequencing further demonstrated the presence of these three genes in five non-sequenced MTC bacteria. Among mycobacteria, the combination of Cel6, Cel12 and CBD2 was unique to MTC members, with the exception of Mycobacterium bovis BCG Pasteur, which lacked CBD2. RT-PCR in M. tuberculosis H37Rv indicated that the three cellulose-targeting genes were transcribed into mRNA. The present work shows that MTC organisms are the sole mycobacteria among very few organisms to encode the three cellulose-targeting genes CBD2, Cel6 and Cel12. Our data point toward a unique, yet unknown, relationship with non-plant cellulose-producing hosts such as amoebae.

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Melzi, Silvia, Guillaume Marcy, Cyril Degletagne, and Christelle Peyron. "Analyses transcriptomiques à cellule unique et à noyau unique de l’hypothalamus dans un état neuro-inflammatoire induit par le LPS." Médecine du Sommeil 20, no. 1 (March 2023): 5–6. http://dx.doi.org/10.1016/j.msom.2023.01.155.

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Tanaka, Takeshi, Shinsuke Fujiwara, Shingo Nishikori, Toshiaki Fukui, Masahiro Takagi, and Tadayuki Imanaka. "A Unique Chitinase with Dual Active Sites and Triple Substrate Binding Sites from the Hyperthermophilic Archaeon Pyrococcus kodakaraensis KOD1." Applied and Environmental Microbiology 65, no. 12 (December 1, 1999): 5338–44. http://dx.doi.org/10.1128/aem.65.12.5338-5344.1999.

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ABSTRACT We have found that the hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1 produces an extracellular chitinase. The gene encoding the chitinase (chiA) was cloned and sequenced. ThechiA gene was found to be composed of 3,645 nucleotides, encoding a protein (1,215 amino acids) with a molecular mass of 134,259 Da, which is the largest among known chitinases. Sequence analysis indicates that ChiA is divided into two distinct regions with respective active sites. The N-terminal and C-terminal regions show sequence similarity with chitinase A1 from Bacillus circulans WL-12 and chitinase from Streptomyces erythraeus (ATCC 11635), respectively. Furthermore, ChiA possesses unique chitin binding domains (CBDs) (CBD1, CBD2, and CBD3) which show sequence similarity with cellulose binding domains of various cellulases. CBD1 was classified into the group of family V type cellulose binding domains. In contrast, CBD2 and CBD3 were classified into that of the family II type. chiA was expressed inEscherichia coli cells, and the recombinant protein was purified to homogeneity. The optimal temperature and pH for chitinase activity were found to be 85°C and 5.0, respectively. Results of thin-layer chromatography analysis and activity measurements with fluorescent substrates suggest that the enzyme is an endo-type enzyme which produces a chitobiose as a major end product. Various deletion mutants were constructed, and analyses of their enzyme characteristics revealed that both the N-terminal and C-terminal halves are independently functional as chitinases and that CBDs play an important role in insoluble chitin binding and hydrolysis. Deletion mutants which contain the C-terminal half showed higher thermostability than did N-terminal-half mutants and wild-type ChiA.

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Wertman, Annette, Andrew V. Wister, and Barbara A. Mitchell. "On and Off the Mat: Yoga Experiences of Middle-Aged and Older Adults." Canadian Journal on Aging / La Revue canadienne du vieillissement 35, no. 2 (April 18, 2016): 190–205. http://dx.doi.org/10.1017/s0714980816000155.

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RÉSUMÉCet article examine les différences potentielles dans la pratique du yoga entre les adultes d’âge moyen et les adultes plus âgés. Un modèle Croyance à Santé/Parcours de Vie encadre cette recherche, et une stratégie analytique de méthodes mixtes est utilisée pour examiner les voies de parcours de vie dans le yoga, et la motivation à la pratique, ainsi que les obstacles perçus et les bénéfices pour la santé. Pour les analyses quantitatives, un échantillon de convenance de 452 participants a été recueilli au moyen d’un questionnaire en ligne. Pour les analyses qualitatives, des entrevues en face-à-face ont été menées auprès d’un sous-ensemble de 20 participants. Des différences uniques se sont manifestées entre les groupes d’âge (à la fois à l’âge actuel et à l’âge du démarrage de yoga), ainsi que par sexe pour certaines voies, les raisons / motivations, et les obstacles à s’engager dans le yoga, ainsi que pour les bénéfices de santé perçues. En outre, les résultats soulignent l’importance des points de repère d’information et des liens sociaux qui affectent la façon dont les individus adoptent et éprouvent le yoga. On discute les implications en ce qui concern les programmes à la promotion de la santé qui ciblent les adultes âgés.

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Elisashvili, Vladimir, Eka Metreveli, Tamar Khardziani, Kakha Sokhadze, Aza Kobakhidze, and Eva Kachlishvili. "Review of Recent Advances in the Physiology of the Regulation of Cellulase and Xylanase Production by Basidiomycetes." Energies 16, no. 11 (May 28, 2023): 4382. http://dx.doi.org/10.3390/en16114382.

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The potential of wood-rotting and litter-deconstructing basidiomycetes to convert lignocellulose into a wide variety of products has been extensively studied. In particular, wood-rotting basidiomycete secretomes are attracting much attention from researchers and biotechnology companies due to their ability to produce extracellular hydrolytic and oxidative enzymes that effectively degrade cellulose, hemicellulose, and lignin of plant biomass. An analysis of the available literature data shows that Basidiomycota fungi, which are most adapted to the depolymerization of plant polysaccharides, are promising but so far unexploited sources of new hydrolytic enzymes. The review summarizes the latest data on the great variety, common features, and unique properties of individual fungi and the production of cellulases and xylanases by various physiological and ecological groups of basidiomycetes. The most important microbial cellulase-producing strains for submerged and solid-phase fermentation, as well as the main substrates, including the use of agro-industrial waste, are considered. It highlights ways to increase both cellulase and xylanase expression levels and the cost-effectiveness of producing these enzymes for various biotechnological applications. It is anticipated that this review will be particularly useful to novice scientists working in the lignocellulose biorefinery, as it describes current knowledge and issues related to the production and regulation of polysaccharide hydrolyzing enzyme synthesis.

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López-Contreras, Ana M., Krisztina Gabor, Aernout A. Martens, Bernadet A. M. Renckens, Pieternel A. M. Claassen, John van der Oost, and Willem M. de Vos. "Substrate-Induced Production and Secretion of Cellulases by Clostridium acetobutylicum." Applied and Environmental Microbiology 70, no. 9 (September 2004): 5238–43. http://dx.doi.org/10.1128/aem.70.9.5238-5243.2004.

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ABSTRACT Clostridium acetobutylicum ATCC 824 is a solventogenic bacterium that grows heterotrophically on a variety of carbohydrates, including glucose, cellobiose, xylose, and lichenan, a linear polymer of β-1,3- and β-1,4-linked β-d-glucose units. C. acetobutylicum does not degrade cellulose, although its genome sequence contains several cellulase-encoding genes and a complete cellulosome cluster of cellulosome genes. In the present study, we demonstrate that a low but significant level of induction of cellulase activity occurs during growth on xylose or lichenan. The celF gene, located in the cellulosome-like gene cluster and coding for a unique cellulase that belongs to glycoside hydrolase family 48, was cloned in Escherichia coli, and antibodies were raised against the overproduced CelF protein. A Western blot analysis suggested a possible catabolite repression by glucose or cellobiose and an up-regulation by lichenan or xylose of the extracellular production of CelF by C. acetobutylicum. Possible reasons for the apparent inability of C. acetobutylicum to degrade cellulose are discussed.

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Abou Rachid, S., E. Nigi, A. Shiba, and V. Zhukova. "Following Neuropathic Pain Route: Glial Alteration in the Thalamus." Douleur et Analgésie 32, no. 4 (December 2019): 221–23. http://dx.doi.org/10.3166/dea-2020-0082.

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Les cellules gliales jouent un rôle important dans l’initiation et la maintenance de la sensation de la douleur. La plupart des études portant sur ce sujet ont été réalisées au niveau de la moelle épinière et relativement peu au niveau supraspinal et notamment thalamique. Les noyaux thalamiques traitent les messages nociceptifs avant d’être adressés au cortex ; en particulier, le noyau thalamique ventropostérolatéral (VPL) est impliqué dans les aspects sensoriels et discriminatifs du traitement de la douleur. L’article présenté par L. Blaszczyk et al. révèle les nouveaux rôles de la microglie et des astrocytes situés dans le VPL et impliqués dans la douleur neuropathique. Dans cette étude, Blasczyk et al. ont utilisé une ligation du nerf spinal L5/L6 chez le rat comme modèle du douleur neuropathique. Des allodynies statiques mécaniques et une hyperalgésie ont été observées chez les animaux après la chirurgie. Plusieurs analyses ont ensuite été effectuées sur les cellules du VPL. Une analyse morphométrique fondée sur les marqueurs gliaux, combinée à un comptage conventionnel et stéréologique de cellules, a indiqué une diminution transitoire de la population de microglies après 14 jours et suggère également une hypertrophie des astrocytes au 28e jour. La réactivité des microglies a été évaluée en utilisant leurs marqueurs spécifiques au 14e jour. Ces résultats révèlent un modèle d’activation séquentiel, sans précédent, des microglies et des astrocytes, pouvant aider à découvrir leur rôle dans l’apparition mais également le maintien de ce dysfonctionnement somatosensoriel.

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Khaffou, Moulouda, Mohamed Raji, and Moha El-Ayachi. "Apport d’analyse des données géophysique et géodésique sur L’évolution dynamique du Système de Rift Est Africain." SHS Web of Conferences 175 (2023): 01021. http://dx.doi.org/10.1051/shsconf/202317501021.

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Le Système de Rift Est Africain SREA constitue une région d’intérêt géologique et géodynamique majeure, offrant des opportunités uniques pour étudier les processus de rift continental. Cette étude vise à caractériser le contexte géologique et géodynamique du SREA en utilisant des données géomatiques et géophysiques. Les données géomatiques ont permis d’identifier les formations rocheuses, les failles actives et les caractéristiques topographiques associées, fournissant ainsi une compréhension approfondie de la région d’étude. En complément, des techniques géophysiques avancées ont été appliquées pour obtenir des informations sur la structure crustale et la lithosphère sous-jacente du SREA. L’analyse des données nous permet de simuler ces processus à grande échelle, mettant en évidence l’impact du mouvement de rotation des plaques sur le développement global de SERA. Les résultats de ces analyses révèlent l’architecture complexe du système de rift, indique que le SREA est caractérisé par plusieurs bassins sédimentaires et segments de rift actifs, avec l’identification de failles majeures et mineures jouant un rôle essentiel dans la déformation et l’évolution de la région. Cette étude fournit une analyse du contexte géologique et géodynamique du Système de Rift Est Africain, en utilisant une approche combinée de données géomatiques et géophysiques.

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Pérol, M., and D. Pérol. "Contribution des méta-analyses au traitement des cancers bronchiques non à petites cellules avancés ou métastatiques." Revue de Pneumologie Clinique 60, no. 1 (February 2004): 29–37. http://dx.doi.org/10.1016/s0761-8417(04)72080-5.

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Walton, Scott, and Eric Meyer. "Interpreting Cellular Coverage for Transportation Applications." Transportation Research Record: Journal of the Transportation Research Board 1826, no. 1 (January 2003): 32–36. http://dx.doi.org/10.3141/1826-05.

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The process was developed for collecting and analyzing cellular coverage data by applying the results of analysis to analog cellular coverage in the state of Kansas. The type of analysis that is appropriate depends on the purpose for which the information is to be used. Two types of analyses were examined—fixed coverage and mobile coverage. Fixed coverage analysis is needed for such functions as automatic collision notification in which any individual cellular connection can occur from a fixed location. The study showed that the fixed cellular coverage of the Kansas state highway system was good. Only 0.4% of the highway by length had inadequate signal strength for using a 3-W phone (a typical car phone) and 1.7% for using a 0.6-W phone (a typical handheld unit). In contrast, the mobile coverage analysis identified numerous areas where a call from a moving vehicle would be severely limited in duration. This type of analysis is needed for applications such as communications for emergency medical services, for which a vehicle must sustain continuous communications. For example, more than 9% of the state highways by length cannot sustain a call of 30 min with a 0.6-W phone, and in some areas the percentage is considerably higher. For certain applications, this difference may simply translate to inconvenience, but for other purposes it can be very important. The results of the two types of analyses highlight different characteristics of the coverage footprint; one addresses absolute coverage and the other continuity of coverage. The results of the analysis technique relate more directly to the unique characteristics of wireless communications utilization in transportation applications.

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Chiu, Kuo-Shou. "Existence and Global Exponential Stability of Equilibrium for Impulsive Cellular Neural Network Models with Piecewise Alternately Advanced and Retarded Argument." Abstract and Applied Analysis 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/196139.

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We introduce impulsive cellular neural network models with piecewise alternately advanced and retarded argument (in short IDEPCA). The model with the advanced argument is system with strong anticipation. Some sufficient conditions are established for the existence and global exponential stability of a unique equilibrium. The approaches are based on employing Banach’s fixed point theorem and a new IDEPCA integral inequality of Gronwall type. The criteria given are easily verifiable, possess many adjustable parameters, and depend on impulses and piecewise constant argument deviations, which provides exibility for the design and analysis of cellular neural network models. Several numerical examples and simulations are also given to show the feasibility and effectiveness of our results.

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Rojas, Miguel, Laisel Martinez, Marwan Tabbara, Simone Pereira, and Roberto Vazquez-Padron. "The Unique Cellular Ecosystem of Peripheral Human Veins And Arteries: A Single-Cell Transcriptomic Analysis." Annals of Vascular Surgery 97 (November 2023): 270–71. http://dx.doi.org/10.1016/j.avsg.2023.09.050.

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Sudarshan A, Renuka S. Talwar, Reshma S, Shilanjali B, and Dayanand Agsar. "Detection, Screening and Molecular Characterization of Potential Actinobacterium from Lime-dwelling Powder for Extra Cellular Cellulase." International Journal for Research in Applied Sciences and Biotechnology 8, no. 1 (January 16, 2021): 94–106. http://dx.doi.org/10.31033/ijrasb.8.1.11.

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Actinobacteria, conventionally known as actinomycetes are the most unique microorganisms revealing a link between bacteria and fungi. They are highly adaptable to extreme environmental condition and also exhibit a high diversity in metabolic activities. Biochemical, physiological and genetic features are mainly responsible for their higher adoptability to harsh conditions and extra cellular synthesis of wider secondary metabolites in general and enzymes and antibiotics in particular. The limestone quarry and lime powder dwellings are the harsh habitats prevailing in the northern region of Karnataka. These are the typical habitats left behind after the exploration of limestone and lime powder for highly commercial industrial activities such as production of cement and petroleum refining process respectively. In the present investigation, efforts were made to detect cellulolytic actinobacteria from lime powder dwellings. Actinobacteria confirmed by the basic colony characters, microscopic features, biochemical and physiological properties were screened for the potential cellulolytic activity. In all 54 isolates of actinobacteria were detected and screened to obtain three best cellulolytic actinobacteria, namely DSA22, DSA38 and DSA39. The maximum zone of hydrolysis on carboxymethylcellulose medium was an important criterion to screen the best cellulolytic isolates of actinobacteria. Further, the three best isolates of cellulolytic actinobacteria were screened for maximum production of extra cellular cellulase. The isolate DSA22 with higher enzyme activity (12 IU) was subjected to molecular characterization. Based on 16s rRNA analysis (BioEra Laboratory, Pune, Maharashtra) an isolate DSA 22 was identified as Streptomyces enissocaesiles.

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Linial, Michal, and Richard H. Scheller. "A Unique Neurofilament from Torpedo Electric Lobe: Sequence, Expression, and Localization Analysis." Journal of Neurochemistry 54, no. 3 (March 1990): 762–70. http://dx.doi.org/10.1111/j.1471-4159.1990.tb02316.x.

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Cernkovich, Stephen A., Catherine E. Kaukinen, and Peggy C. Giordano. "Les types de délinquantes : une étude longitudinale des causes et des conséquences1." Criminologie 38, no. 1 (October 17, 2005): 103–38. http://dx.doi.org/10.7202/011487ar.

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Résumé L’existence de différences sexuelles sur le plan du comportement antisocial est un fait largement répandu et accepté en criminologie, et ce, depuis la naissance de cette discipline. Bien que depuis une trentaine d’années les chercheurs se soient intéressés plus que jamais à la recherche théorique et empirique du comportement antisocial des femmes, force est de constater que les criminologues n’ont pas encore pleinement exploré la diversité des types de délinquance féminine. Notre examen des causes et des conséquences de la délinquance féminine s’appuie sur trois postulats de base : 1) la population délinquante est hétérogène 2) l’existence de types distincts de délinquantes est le produit de processus causaux qui sont à la fois communs et distincts et 3) les conséquences à long terme de la délinquance varient selon le type de délinquantes. Les données autorapportées ont été recueillies à partir d’un échantillon de répondantes interrogées en 1982, alors qu’elles étaient adolescentes, et subséquemment en 1992, alors qu’elles avaient atteint l’âge adulte. Nos analyses ont décelé des facteurs étiologiques uniques et communs à l’ensemble des types de délinquantes, ainsi que des événements de vie variant en fonction d’une diversité de dimensions comportementales, personnelles et interpersonnelles. Nos résultats font ressortir qu’une consommation de drogues durant l’adolescence a des effets particulièrement délétères chez les femmes lors du passage de l’adolescence à l’âge adulte.

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Liang, Bin, Mark A. Gregory, and Shuo Li. "Latency Analysis for Mobile Cellular Network uRLLC Services." Journal of Telecommunications and the Digital Economy 10, no. 3 (September 21, 2022): 39–57. http://dx.doi.org/10.18080/jtde.v10n3.447.

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The fifth generation (5G) mobile network technologies include ultra-Reliable Low Latency Communications (uRLLC) capability. To fully exploit uRLLC, distributed Multi- access Edge Computing (MEC) is being developed and introduced at the network edge with an architecture that supports applications and services. Some of the MEC applications will benefit from uRLLC, including virtual reality, augmented reality, education, health, online gaming, automatic manufacturing and Vehicle-to-everything. However, unique challenges and opportunities exist for 5G cellular networks and MEC due to a range of factors, including end-user device mobility and the implementation of the network Control Plane (CP) and User Plane (UP). In this regard, there is a need to optimize protocols and network architecture. This paper investigates latency and related network elements in the next generation mobile cellular network. We also analyze the 5G network latency in the CP and UP. Finally, the paper identifies protocol optimization considerations for MEC integration with 5G to achieve low end-to-end latency.

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Larroque, Mathieu, Roland Barriot, Arnaud Bottin, Annick Barre, Pierre Rougé, Bernard Dumas, and Elodie Gaulin. "The unique architecture and function of cellulose-interacting proteins in oomycetes revealed by genomic and structural analyses." BMC Genomics 13, no. 1 (2012): 605. http://dx.doi.org/10.1186/1471-2164-13-605.

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Lott, Kaylen, Jun Li, John C. Fisk, Hao Wang, John M. Aletta, Jun Qu, and Laurie K. Read. "Global proteomic analysis in trypanosomes reveals unique proteins and conserved cellular processes impacted by arginine methylation." Journal of Proteomics 91 (October 2013): 210–25. http://dx.doi.org/10.1016/j.jprot.2013.07.010.

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Legrès, Luc G. "Les applications de la microdissection laser en histologie." médecine/sciences 35, no. 11 (November 2019): 871–79. http://dx.doi.org/10.1051/medsci/2019166.

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La microdissection laser permet d’isoler des cellules, phénotypiquement identiques, à partir d’une lame de microscope portant un tissu biologique, dans l’optique de réaliser des analyses moléculaires différentielles, spécifiques de ces populations isolées. La technologie s’applique notamment en oncologie, pour préciser des mécanismes moléculaires qui permettent d’adapter un traitement lié au diagnostic et à la recherche en biologie, mais aussi en criminalistique, pour la sélection tissulaire, en neurologie pour des études post-mortem sur des patients atteints de maladie d’Alzheimer, pour des études de clonalité à partir de cultures cellulaires, et en cytogénétique, pour décrypter les réarrangements chromosomiques. C’est le chaînon manquant entre observations cliniques et mécanismes physiologiques intrinsèques des tissus biologiques. Nous aborderons dans cette revue ses applications majeures.

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Braten, Ori, Ido Livneh, Tamar Ziv, Arie Admon, Izhak Kehat, Lilac H. Caspi, Hedva Gonen, et al. "Numerous proteins with unique characteristics are degraded by the 26S proteasome following monoubiquitination." Proceedings of the National Academy of Sciences 113, no. 32 (July 6, 2016): E4639—E4647. http://dx.doi.org/10.1073/pnas.1608644113.

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The “canonical” proteasomal degradation signal is a substrate-anchored polyubiquitin chain. However, a handful of proteins were shown to be targeted following monoubiquitination. In this study, we established—in both human and yeast cells—a systematic approach for the identification of monoubiquitination-dependent proteasomal substrates. The cellular wild-type polymerizable ubiquitin was replaced with ubiquitin that cannot form chains. Using proteomic analysis, we screened for substrates that are nevertheless degraded under these conditions compared with those that are stabilized, and therefore require polyubiquitination for their degradation. For randomly sampled representative substrates, we confirmed that their cellular stability is in agreement with our screening prediction. Importantly, the two groups display unique features: monoubiquitinated substrates are smaller than the polyubiquitinated ones, are enriched in specific pathways, and, in humans, are structurally less disordered. We suggest that monoubiquitination-dependent degradation is more widespread than assumed previously, and plays key roles in various cellular processes.

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Kaur, Dr Dalveer, and Neeraj Kumar. "Performance Analysis of Handoff in CDMA Cellular System." INTERNATIONAL JOURNAL OF COMPUTERS & TECHNOLOGY 9, no. 3 (July 25, 2013): 1119–26. http://dx.doi.org/10.24297/ijct.v9i3.3337.

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Handoff mechanism is extremely important in cellular network because of the cellular architecture employed to maximize spectrum utilization. This unique feature has driven the rapid growth in the mobile network industry, changing it from a new technology into a massive industry within less than two decades. Handoff is the essential functionality for dealing with the mobility of the mobile users. This paper shows the soft handoff effects on the uplink direction of IS-95 CDMA networks is carried out, leading to optimize soft handoff for capacity under perfect power control approach. In practical systems, there is a nonzero handoff completion delay and soft handoff provides the required robustness to delays, although it comes at the expense of additional network resources. Thus, there is a tradeoff between the extent of soft handoff required and the handoff execution delay. This paper presents an analytical framework to study this tradeoff and also discuss simulation results simulated with the help of Matlab. For this, handoff dropping probability is minimized up to 0.1%.

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Salem, Mohamed, P. Brett Kenney, Caird E. Rexroad, and Jianbo Yao. "Microarray gene expression analysis in atrophying rainbow trout muscle: a unique nonmammalian muscle degradation model." Physiological Genomics 28, no. 1 (December 2006): 33–45. http://dx.doi.org/10.1152/physiolgenomics.00114.2006.

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Muscle atrophy is a physiological response to diverse physiological and pathological conditions that trigger muscle deterioration through specific cellular mechanisms. Despite different signals, the biochemical changes in atrophying muscle share many common cascades. Muscle deterioration as a physiological response to the energetic demands of fish vitellogenesis represents a unique model for studying the mechanisms of muscle degradation in non-mammalian animals. A salmonid microarray, containing 16,006 cDNAs, was used to study the transcriptome response to atrophy of fast-switch muscles from gravid rainbow trout compared with sterile fish. Eighty-two unique transcripts were upregulated and 120 transcripts were downregulated in atrophying muscles. Transcripts having gene ontology identifiers were grouped according to their functions. Muscle deterioration was associated with elevated expression of genes involved in the catheptic and collagenase proteolytic pathways; the aerobic production, buffering, and utilization of ATP; and growth arrest; whereas atrophying muscle showed downregulation of genes encoding a serine proteinase inhibitor, enzymes of anaerobic respiration, muscle proteins as well as genes required for RNA and protein biosynthesis/processing. Therefore, gene transcription of the trout muscle atrophy changed in a manner similar to mammalian muscle atrophy. These changes result in an arrest of normal cell growth, protein degradation, and decreased glycolytic cellular respiration that is characteristic of the fast-switch muscle. For the first time, other changes/mechanisms unique to fish were discussed including genes associated with muscle atrophy.

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Millet, Larry J., Anika Jain, and Martha U. Gillette. "Less Is More: Oligomer Extraction and Hydrothermal Annealing Increase PDMS Adhesion Forces for Materials Studies and for Biology-Focused Microfluidic Applications." Micromachines 14, no. 1 (January 14, 2023): 214. http://dx.doi.org/10.3390/mi14010214.

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Cues in the micro-environment are key determinants in the emergence of complex cellular morphologies and functions. Primary among these is the presence of neighboring cells that form networks. For high-resolution analysis, it is crucial to develop micro-environments that permit exquisite control of network formation. This is especially true in cell science, tissue engineering, and clinical biology. We introduce a new approach for assembling polydimethylsiloxane (PDMS)-based microfluidic environments that enhances cell network formation and analyses. We report that the combined processes of PDMS solvent-extraction and hydrothermal annealing create unique conditions that produce high-strength bonds between solvent-extracted PDMS (E-PDMS) and glass—properties not associated with conventional PDMS. Extraction followed by hydrothermal annealing removes unbound oligomers, promotes polymer cross-linking, facilitates covalent bond formation with glass, and retains the highest biocompatibility. Herein, our extraction protocol accelerates oligomer removal from 5 to 2 days. Resulting microfluidic platforms are uniquely suited for cell-network studies owing to high adhesion forces, effectively corralling cellular extensions and eliminating harmful oligomers. We demonstrate the simple, simultaneous actuation of multiple microfluidic domains for invoking ATP- and glutamate-induced Ca2+ signaling in glial-cell networks. These E-PDMS modifications and flow manipulations further enable microfluidic technologies for cell-signaling and network studies as well as novel applications.

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Jeudy, Sandra, Audrey Lartigue, Jean-Michel Claverie, and Chantal Abergel. "Dissecting the Unique Nucleotide Specificity of Mimivirus Nucleoside Diphosphate Kinase." Journal of Virology 83, no. 14 (May 13, 2009): 7142–50. http://dx.doi.org/10.1128/jvi.00511-09.

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ABSTRACT The analysis of the Acanthamoeba polyphaga mimivirus genome revealed the first virus-encoded nucleoside diphosphate kinase (NDK), an enzyme that is central to the synthesis of RNA and DNA, ubiquitous in cellular organisms, and well conserved among the three domains of life. In contrast with the broad specificity of cellular NDKs for all types of ribo- and deoxyribonucleotides, the mimivirus enzyme exhibits a strongly preferential affinity for deoxypyrimidines. In order to elucidate the molecular basis of this unique substrate specificity, we determined the three-dimensional (3D) structure of the Acanthamoeba polyphaga mimivirus NDK alone and in complex with various nucleotides. As predicted from a sequence comparison with cellular NDKs, the 3D structure of the mimivirus enzyme exhibits a shorter Kpn loop, previously recognized as a main feature of the NDK active site. The structure of the viral enzyme in complex with various nucleotides also pinpointed two residue changes, both located near the active site and specific to the viral NDK, which could explain its stronger affinity for deoxynucleotides and pyrimidine nucleotides. The role of these residues was explored by building a set of viral NDK variants, assaying their enzymatic activities, and determining their 3D structures in complex with various nucleotides. A total of 26 crystallographic structures were determined at resolutions ranging from 2.8 Å to 1.5 Å. Our results suggest that the mimivirus enzyme progressively evolved from an ancestral NDK under the constraints of optimizing its efficiency for the replication of an AT-rich (73%) viral genome in a thymidine-limited host environment.

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Catlow, K., J. A. Deakin, M. Delehedde, D. G. Fernig, J. T. Gallagher, M. S. G. Pavão, and M. Lyon. "Hepatocyte growth factor/scatter factor and its interaction with heparan sulphate and dermatan sulphate." Biochemical Society Transactions 31, no. 2 (April 1, 2003): 352–53. http://dx.doi.org/10.1042/bst0310352.

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Hepatocyte growth factor (HGF)/scatter factor (SF) is a unique growth factor, in that it binds both heparan sulphate (HS) and dermatan sulphate (DS). The sequences in HS and DS that specifically interact with and modulate HGF/SF activity have not yet been fully identified. Ascidian DS, which uniquely possesses O-sulphation at C-6 (and not C-4) of its N-acetylgalactosamine unit, was analysed for HGF/SF-binding activity in the biosensor. The kinetic analysis revealed a strong, biologically relevant interaction with an equilibrium dissociation constant (Kd) of approx. 1 nM. An Erk activation assay also demonstrated stimulation of the MAP kinase pathway downstream of the Met receptor following addition of both HGF/SF and ascidian DS to the glycosaminoglycan-deficient CHO-745 mutant cell line. Furthermore, the activation of Met and the MAP kinase pathway by HGF/SF and ascidian DS leads to a cellular response in the form of migration.

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Srivastava, Avi, Laraib Malik, Hirak Sarkar, and Rob Patro. "A Bayesian framework for inter-cellular information sharing improves dscRNA-seq quantification." Bioinformatics 36, Supplement_1 (July 1, 2020): i292—i299. http://dx.doi.org/10.1093/bioinformatics/btaa450.

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Abstract Motivation Droplet-based single-cell RNA-seq (dscRNA-seq) data are being generated at an unprecedented pace, and the accurate estimation of gene-level abundances for each cell is a crucial first step in most dscRNA-seq analyses. When pre-processing the raw dscRNA-seq data to generate a count matrix, care must be taken to account for the potentially large number of multi-mapping locations per read. The sparsity of dscRNA-seq data, and the strong 3’ sampling bias, makes it difficult to disambiguate cases where there is no uniquely mapping read to any of the candidate target genes. Results We introduce a Bayesian framework for information sharing across cells within a sample, or across multiple modalities of data using the same sample, to improve gene quantification estimates for dscRNA-seq data. We use an anchor-based approach to connect cells with similar gene-expression patterns, and learn informative, empirical priors which we provide to alevin’s gene multi-mapping resolution algorithm. This improves the quantification estimates for genes with no uniquely mapping reads (i.e. when there is no unique intra-cellular information). We show our new model improves the per cell gene-level estimates and provides a principled framework for information sharing across multiple modalities. We test our method on a combination of simulated and real datasets under various setups. Availability and implementation The information sharing model is included in alevin and is implemented in C++14. It is available as open-source software, under GPL v3, at https://github.com/COMBINE-lab/salmon as of version 1.1.0.

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Beske, Oren, Jinjiao Guo, Jianren Li, Daniel Bassoni, Kimberly Bland, Holly Marciniak, Mike Zarowitz, Vladimir Temov, Ilya Ravkin, and Simon Goldbard. "A Novel Encoded Particle Technology that Enables Simultaneous Interrogation of Multiple Cell Types." Journal of Biomolecular Screening 9, no. 3 (April 2004): 173–85. http://dx.doi.org/10.1177/1087057103260088.

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The authors have developed a cellular analysis platform, based on encoded microcarriers, that enables the multiplexed analysis of a diverse range of cellular assays. At the core of this technology are classes of microcarriers that have unique, identifiable codes that are deciphered using CCD-based imaging and subsequent image analysis. The platform is compatible with a wide variety of cellular imaging-based assays, including calcium flux, reporter gene activation, cytotoxicity, and proliferation. In addition, the platform is compatible with both colorimetric and fluorescent readouts. Notably, this technology has the unique ability to multiplex different cell lines in a single microplate well, enabling scientists to perform assays and data analysis in novel ways.

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Hart, Jane, Mathias Ackermann, Gamini Jayawardane, George Russell, David M. Haig, Hugh Reid, and James P. Stewart. "Complete sequence and analysis of the ovine herpesvirus 2 genome." Journal of General Virology 88, no. 1 (January 1, 2007): 28–39. http://dx.doi.org/10.1099/vir.0.82284-0.

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Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to Alcelaphine herpesvirus 1 (AlHV-1). Here, the cloning, sequencing and analysis of the complete OvHV-2 genome derived from a lymphoblastoid cell line from an affected cow (BJ1035) are reported. The unique portion of the genome consists of 130 930 bp, with a mean G+C content of 52 mol%. The unique DNA is flanked by multiple copies of terminal repeat elements 4205 bp in length, with a mean G+C content of 72 mol%. Analysis revealed 73 open reading frames (ORFs), the majority (62) of which showed homology to other gammaherpesvirus genes. A further subset of nine ORFs is shared with only the related AlHV-1. Three ORFs are entirely unique to OvHV-2, including a spliced homologue of cellular interleukin-10 that retains the exon structure of the cellular gene. The sequence of OvHV-2 is a critical first step in the study of the pathogenesis and treatment of MCF.

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Belova, Svetlana E., Daniil G. Naumoff, Natalia E. Suzina, Vladislav V. Kovalenko, Nataliya G. Loiko, Vladimir V. Sorokin, and Svetlana N. Dedysh. "Building a Cell House from Cellulose: The Case of the Soil Acidobacterium Acidisarcina polymorpha SBC82T." Microorganisms 10, no. 11 (November 14, 2022): 2253. http://dx.doi.org/10.3390/microorganisms10112253.

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Acidisarcina polymorpha SBC82T is a recently described representative of the phylum Acidobacteriota from lichen-covered tundra soil. Cells of this bacterium occur within unusual saccular chambers, with the chamber envelope formed by tightly packed fibrils. These extracellular structures were most pronounced in old cultures of strain SBC82T and were organized in cluster-like aggregates. The latter were efficiently destroyed by incubating cell suspensions with cellulase, thus suggesting that they were composed of cellulose. The diffraction pattern obtained for 45-day-old cultures of strain SBC82T by using small angle X-ray scattering was similar to those reported earlier for mature wood samples. The genome analysis revealed the presence of a cellulose biosynthesis locus bcs. Cellulose synthase key subunits A and B were encoded by the bcsAB gene whose close homologs are found in genomes of many members of the order Acidobacteriales. More distant homologs of the acidobacterial bcsAB occurred in representatives of the Proteobacteria. A unique feature of bcs locus in strain SBC82T was the non-orthologous displacement of the bcsZ gene, which encodes the GH8 family glycosidase with a GH5 family gene. Presumably, these cellulose-made extracellular structures produced by A. polymorpha have a protective function and ensure the survival of this acidobacterium in habitats with harsh environmental conditions.

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Kala, Srikant Manas, Vanlin Sathya, Kunal Dahiya, Teruo Higashino, and Hirozumi Yamaguchi. "Identification and Analysis of a Unique Cell Selection Phenomenon in Public Unlicensed Cellular Networks Through Machine Learning." IEEE Access 10 (2022): 87282–301. http://dx.doi.org/10.1109/access.2022.3199409.

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Rana, Md Sohel, Md Abdur Rahim, Md Pervez Mosharraf, Md Fazlul Karim Tipu, Jakir Ahmed Chowdhury, Mohammad Rashedul Haque, Shaila Kabir, Md Shah Amran, and Abu Asad Chowdhury. "Morphological, Spectroscopic and Thermal Analysis of Cellulose Nanocrystals Extracted from Waste Jute Fiber by Acid Hydrolysis." Polymers 15, no. 6 (March 20, 2023): 1530. http://dx.doi.org/10.3390/polym15061530.

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Natural cellulose, a sustainable bioresource, is highly abundant in nature. Cellulosic materials, particularly those that explore and employ such materials for industrial use, have recently attracted significant global attention in the field of material science because of the unique properties of cellulose. The hydroxyl groups enable the formation of intra- and inter-molecular hydrogen bonding and the arrangement of cellulose chains in a highly ordered crystalline zone, with the remaining disordered structure referred to as an amorphous region. The crystalline areas of cellulose are well-known as cellulose nanocrystals (CNCs). In the present study, we extracted CNCs from pure cellulose isolated from waste jute fibers by sulfuric acid hydrolysis, followed by characterization. Pure cellulose was isolated from jute fibers by treating with sodium hydroxide (20% w/w) and anthraquinone (0.5%) solution at 170 °C for 2 h, followed by bleaching with chlorine dioxide and hydrogen peroxide solution. CNCs were isolated from pure cellulose by treating with different concentrations (58% to 62%) of sulfuric acid at different time intervals (20 min to 45 min). The FTIR study of the CNCs reveals no peak at 1738 cm−1, which confirms the absence of hemicellulose in the samples. The CNCs obtained after 45 min of acid hydrolysis are rod-shaped, having an average length of 800 ± 100 nm and width of 55 ± 10 nm, with a high crystallinity index (90%). Zeta potential significantly increased due to the attachment of SO42− ions on the surface of CNC from −1.0 mV to about −30 mV, with the increment of the reaction time from 20 min to 45 min, which proved the higher stability of CNC suspension. Crystallinity increased from 80% to 90% when the reaction time was increased from 20 to 45 min, respectively, while a crystallite size from 2.705 to 4.56 nm was obtained with an increment of the acid concentration. Acid hydrolysis enhanced crystallinity but attenuated the temperature corresponding to major decomposition (Tmax) at 260 °C and the beginning of degradation (Ti) at 200 °C due to the attachment of SO42− ions on the surface, which decreased the thermal stability of CNC. The second degradation at 360 °C indicated the stable crystal structure of CNC. The endothermic peak at 255 °C in the DTA study provided evidence of sulfated nanocrystal decomposition and the recrystallization of cellulose I to cellulose II, the most stable structure among the other four celluloses. The proposed easy-to-reproduce method can successfully and efficiently produce CNCs from waste jute fibers in a straightforward way.

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Diamond, R. H., D. E. Cressman, T. M. Laz, C. S. Abrams, and R. Taub. "PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth." Molecular and Cellular Biology 14, no. 6 (June 1994): 3752–62. http://dx.doi.org/10.1128/mcb.14.6.3752-3762.1994.

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PRL-1 is a particularly interesting immediate-early gene because it is induced in mitogen-stimulated cells and regenerating liver but is constitutively expressed in insulin-treated rat H35 hepatoma cells, which otherwise show normal regulation of immediate-early genes. PRL-1 is expressed throughout the course of hepatic regeneration, and its expression is elevated in a number of tumor cell lines. Sequence analysis reveals that PRL-1 encodes a 20-kDa protein with an eight-amino-acid consensus protein tyrosine phosphatase (PTPase) active site. PRL-1 is able to dephosphorylate phosphotyrosine substrates, and mutation of the active-site cysteine residue abolishes this activity. As PRL-1 has no homology to other PTPases outside the active site, it is a new type of PTPase. PRL-1 is located primarily in the cell nucleus. Stably transfected cells which overexpress PRL-1 demonstrate altered cellular growth and morphology and a transformed phenotype. It appears that PRL-1 is important in normal cellular growth control and could contribute to the tumorigenicity of some cancer cells.

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Diamond, R. H., D. E. Cressman, T. M. Laz, C. S. Abrams, and R. Taub. "PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth." Molecular and Cellular Biology 14, no. 6 (June 1994): 3752–62. http://dx.doi.org/10.1128/mcb.14.6.3752.

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PRL-1 is a particularly interesting immediate-early gene because it is induced in mitogen-stimulated cells and regenerating liver but is constitutively expressed in insulin-treated rat H35 hepatoma cells, which otherwise show normal regulation of immediate-early genes. PRL-1 is expressed throughout the course of hepatic regeneration, and its expression is elevated in a number of tumor cell lines. Sequence analysis reveals that PRL-1 encodes a 20-kDa protein with an eight-amino-acid consensus protein tyrosine phosphatase (PTPase) active site. PRL-1 is able to dephosphorylate phosphotyrosine substrates, and mutation of the active-site cysteine residue abolishes this activity. As PRL-1 has no homology to other PTPases outside the active site, it is a new type of PTPase. PRL-1 is located primarily in the cell nucleus. Stably transfected cells which overexpress PRL-1 demonstrate altered cellular growth and morphology and a transformed phenotype. It appears that PRL-1 is important in normal cellular growth control and could contribute to the tumorigenicity of some cancer cells.

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Chen, Xueying, Min Zhao, and Hong Qu. "Cellular Metabolic Network Analysis: Discovering Important Reactions inTreponema pallidum." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/328568.

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T. pallidum, the syphilis-causing pathogen, performs very differently in metabolism compared with other bacterial pathogens. The desire for safe and effective vaccine of syphilis requests identification of important steps inT. pallidum’s metabolism. Here, we apply Flux Balance Analysis to represent the reactions quantitatively. Thus, it is possible to cluster all reactions inT. pallidum. By calculating minimal cut sets and analyzing topological structure for the metabolic network ofT. pallidum, critical reactions are identified. As a comparison, we also apply the analytical approaches to the metabolic network ofH. pylorito find coregulated drug targets and unique drug targets for different microorganisms. Based on the clustering results, all reactions are further classified into various roles. Therefore, the general picture of their metabolic network is obtained and two types of reactions, both of which are involved in nucleic acid metabolism, are found to be essential forT. pallidum. It is also discovered that both hubs of reactions and the isolated reactions in purine and pyrimidine metabolisms play important roles inT. pallidum. These reactions could be potential drug targets for treating syphilis.

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Halitim, P., A. Tissot, L. Boussamet, A. Garcia, C. Fourgeux, P. Lacoste, B. Marie, J. Poschmann, S. Brouard, and L. Berthelot. "Étude de la physiopathologie de la dysfonction chronique du greffon pulmonaire par analyse transcriptomique sur cellule unique d’explants pulmonaires." Revue des Maladies Respiratoires 41, no. 3 (March 2024): 202–3. http://dx.doi.org/10.1016/j.rmr.2024.01.044.

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McGinnis, J. F., P. L. Stepanik, W. Chen, R. Elias, W. Cao, and V. Lerious. "Unique retina cell phenotypes revealed by immunological analysis of recoverin expression in rat retina cells." Journal of Neuroscience Research 55, no. 2 (January 15, 1999): 252–60. http://dx.doi.org/10.1002/(sici)1097-4547(19990115)55:2<252::aid-jnr13>3.0.co;2-n.

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Salnikov, Mikhail Y., Gregory J. Fonseca, and Joe S. Mymryk. "Differences in the Tumor Microenvironment of EBV-Associated Gastric Cancers Revealed Using Single-Cell Transcriptome Analysis." Cancers 15, no. 12 (June 14, 2023): 3178. http://dx.doi.org/10.3390/cancers15123178.

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Epstein–Barr virus (EBV) is a gamma-herpesvirus associated with nearly 10% of gastric cancers (GCs). These EBV-associated GCs (EBVaGCs) are molecularly, histopathologically, and clinically distinct from EBV-negative GCs (EBVnGCs). While viral genes in EBVaGCs contribute to the carcinogenesis process, viral proteins also represent foreign antigens that could trigger enhanced immune responses compared to EBVnGCs. Despite prior investigations of the EBVaGC tumor microenvironment (TME), the cellular composition has not been thoroughly explored. In this study, cellular subpopulations overrepresented in EBVaGCs were identified and molecularly characterized. Genes consistently expressed across both bulk tumor and single-cell RNA sequencing data were highlighted, with the expression across the identified cellular subpopulations analyzed. As expected, based on existing histopathological analysis, EBVaGC is characterized by abundant lymphocytic infiltration of the stroma. Our molecular analysis identified three unique immune cell subpopulations in EBVaGC: T and B cells expressing high levels of proliferation markers and B cells expressing T cell features. The proliferating T cell cluster also expressed markers of follicular T helper cells. Overall, EBVaGC also exhibited unique features indicative of a higher inflammatory response. These substantial differences within the TME suggest that further detailed exploration of the cellular composition of EBVaGCs is needed, which may identify cellular subpopulations and phenotypes associated with patient outcomes.

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