Готові списки джерел за темами / Anaerobe E.coli K12

Добірка наукової літератури з теми "Anaerobe E.coli K12"

Автор: Grafiati
Опубліковано: 6 вересня 2023

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Статті в журналах з теми "Anaerobe E.coli K12"

1

Commodari, Fernando, and Brian K. Hunter. "Production and interconversion of 1,2-propanediol and hydroxyacetone by Escherichia coli." Biochemistry and Cell Biology 67, no. 8 (August 1, 1989): 468–72. http://dx.doi.org/10.1139/o89-074.

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The anaerobic metabolism of marginally lethal levels of [13C]formaldehyde by Escherichia coli (K12, MU352, CRB, and CR63) was followed in vivo by 13C NMR. The products include 1, 2-propanediol. Under aeration, the 1, 2-propanediol is converted to hydroxyacetone. The hydroxyacetone is reconverted to 1, 2-propanediol when aeration is stopped. The process can be cycled by varying the rate of aeration.Key words: hydroxyacetone, 1, 2-propanediol, Escherichia coli, production.
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2

SCHAMBERGER, GERRY P., and FRANCISCO DIEZ-GONZALEZ. "Selection of Recently Isolated Colicinogenic Escherichia coli Strains Inhibitory to Escherichia coli O157:H7." Journal of Food Protection 65, no. 9 (September 1, 2002): 1381–87. http://dx.doi.org/10.4315/0362-028x-65.9.1381.

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Escherichia coli strains were screened for their ability to inhibit E. coli O157:H7. An initial evaluation of 18 strains carrying previously characterized colicins determined that only colicin E7 inhibited all of the E. coli O157:H7 strains tested. A total of 540 strains that had recently been isolated from humans and nine different animal species (cats, cattle, chickens, deer, dogs, ducks, horses, pigs, and sheep) were tested by a flip-plating technique. Approximately 38% of these strains were found to inhibit noncolicinogenic E. coli K12 strains. The percentage of potentially colicinogenic E. coli per animal species ranged from 14% for horse isolates to 64% for sheep strains. Those isolates that inhibited E. coli K12 were screened against E. coli O157:H7, and 42 strains were found to be capable of inhibiting all 22 pathogenic strains tested. None of these 42 strains produced bacteriophages, and only 24 isolates inhibited serotype O157:H7 in liquid culture. The inhibitory activity of these strains was completely eliminated by treatment with proteinase K. When mixtures of these 24 colicinogenic strains were grown in anaerobic continuous culture, the four-strain E. coli O157:H7 population was reduced at a rate of 0.25 log10 cells per ml per h, which was fivefold faster than the washout rate. Two strains originally isolated from cat feces (F16) and human feces (H30) were identified by repetitive sequences polymerase chain reaction as the predominant isolates in continuous cultures. The results of this work indicate that animal species other than cattle can be sources of anti-O157 colicinogenic strains, and these results also lead to the identification of at least two isolates that could potentially be used in preharvest control strategies.
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3

Ralph, E. T., J. R. Guest, and J. Green. "Altering the anaerobic transcription factor FNR confers a hemolytic phenotype on Escherichia coli K12." Proceedings of the National Academy of Sciences 95, no. 18 (September 1, 1998): 10449–52. http://dx.doi.org/10.1073/pnas.95.18.10449.

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4

Mat-Jan, Fairoz, Charling R. Williams, and David P. Clark. "Anaerobic growth defects resulting from gene fusions affecting succinyl-CoA synthetase in Escherichia coli K12." Molecular and General Genetics MGG 215, no. 2 (January 1989): 276–80. http://dx.doi.org/10.1007/bf00339728.

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5

Asare, Paul Tetteh, Katrin Zurfluh, Anna Greppi, Denise Lynch, Clarissa Schwab, Roger Stephan, and Christophe Lacroix. "Reuterin Demonstrates Potent Antimicrobial Activity Against a Broad Panel of Human and Poultry Meat Campylobacter spp. Isolates." Microorganisms 8, no. 1 (January 6, 2020): 78. http://dx.doi.org/10.3390/microorganisms8010078.

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Reuterin is a broad-spectrum antimicrobial system produced by specific strains of Lactobacillus reuteri during anaerobic metabolism of glycerol. Acrolein is the main component responsible for its antimicrobial activity. Here, the sensitivity of Campylobacter jejuni (n = 51) and Campylobacter coli (n = 20) isolates from chicken meat and human stool samples to reuterin was investigated. The minimum inhibitory concentration (MIC) of C. jejuni and C. coli strains was measured between 1.5 and 3.0 µM of acrolein, below the MIC of the sensitive indicator strain Escherichia coli K12 (16.5 µM acrolein). The interaction of C. jejuni N16-1419 and the reuterin-producing L. reuteri PTA5_F13 was studied during 24 h co-cultures with or without glycerol. A high C. jejuni growth was observed in cultures without glycerol. In contrast, C. jejuni growth decreased from 7.3 ± 0.1 log CFU/mL to below detection limit (1 log CFU/mL) during co-cultures added with 28 mM glycerol. This bactericidal effect could be attributed to in situ reuterin production. The low MIC observed and the high sensitivity towards in situ produced reuterin suggests L. reuteri combined with glycerol, as a possible intervention option to reduce Campylobacter in the food chain.
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6

Portnoy, Vasiliy A., Markus J. Herrgård, and Bernhard Ø. Palsson. "Aerobic Fermentation of d-Glucose by an Evolved Cytochrome Oxidase-Deficient Escherichia coli Strain." Applied and Environmental Microbiology 74, no. 24 (October 24, 2008): 7561–69. http://dx.doi.org/10.1128/aem.00880-08.

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ABSTRACT Fermentation of glucose to d-lactic acid under aerobic growth conditions by an evolved Escherichia coli mutant deficient in three terminal oxidases is reported in this work. Cytochrome oxidases (cydAB, cyoABCD, and cbdAB) were removed from the E. coli K12 MG1655 genome, resulting in the ECOM3 (E. coli cytochrome oxidase mutant) strain. Removal of cytochrome oxidases reduced the oxygen uptake rate of the knockout strain by nearly 85%. Moreover, the knockout strain was initially incapable of growing on M9 minimal medium. After the ECOM3 strain was subjected to adaptive evolution on glucose M9 medium for 60 days, a growth rate equivalent to that of anaerobic wild-type E. coli was achieved. Our findings demonstrate that three independently adaptively evolved ECOM3 populations acquired different phenotypes: one produced lactate as a sole fermentation product, while the other two strains exhibited a mixed-acid fermentation under oxic growth conditions with lactate remaining as the major product. The homofermenting strain showed a d-lactate yield of 0.8 g/g from glucose. Gene expression and in silico model-based analyses were employed to identify perturbed pathways and explain phenotypic behavior. Significant upregulation of ygiN and sodAB explains the remaining oxygen uptake that was observed in evolved ECOM3 strains. E. coli strains produced in this study showed the ability to produce lactate as a fermentation product from glucose and to undergo mixed-acid fermentation during aerobic growth.
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7

Beaumont, M. D., and H. M. Hassan. "Characterization of regulatory mutations causing anaerobic derepression of the sodA gene in Escherichia coli K12: cooperation between cis- and trans-acting regulatory loci." Journal of General Microbiology 139, no. 11 (November 1, 1993): 2677–84. http://dx.doi.org/10.1099/00221287-139-11-2677.

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8

Lu, Jingrang, Tammie L. Gerke, Helen Y. Buse, and Nicholas J. Ashbolt. "Development of an Escherichia coli K12-specific quantitative polymerase chain reaction assay and DNA isolation suited to biofilms associated with iron drinking water pipe corrosion products." Journal of Water and Health 12, no. 4 (June 6, 2014): 763–71. http://dx.doi.org/10.2166/wh.2014.203.

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A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABITM internal control was developed based on sequence data encoding the rfb gene cluster. Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. The biofilm detection limit was 103 colony-forming units (CFU) E. coli K12 mL−1, but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. The novel protocol yielded the same sensitivity for drinking water biofilms associated with Fe3O4 (magnetite)-coated SiO2 (quartz) grains and biofilm-surface iron corrosion products from a drinking water distribution system. The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms.
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9

Ung, Porsry, Chanthol Peng, Sokunsreiroat Yuk, Vannak Ann, Hasika Mith, Reasmey Tan, Kazuhiko Miyanaga, and Yasunori Tanji. "Fate of Escherichia coli in dialysis device exposed into sewage influent and activated sludge." Journal of Water and Health 16, no. 3 (April 18, 2018): 380–90. http://dx.doi.org/10.2166/wh.2018.282.

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Abstract Tracing the fate of pathogens in environmental water, particularly in wastewater, with a suitable methodology is a demanding task. We investigated the fate of Escherichia coli K12 in sewage influent and activated sludge using a novel approach that involves the application of a biologically stable dialysis device. The ion concentrations inside the device could reach that of surrounding solution when it was incubated in phosphate buffered saline for 2 h. E. coli K12 above 107 CFU mL−1 (inoculated in distilled water, influent, activated sludge) were introduced into the device and incubated in influent and activated sludge for 10 days. Without indigenous microorganisms, E. coli K12 could survive even with the limited ions and nutrients concentrations in influent and activated sludge. E. coli K12 abundance in influent and activated sludge were reduced by 60 and 85%, respectively, after just 1 day. The establishment of microbial community in wastewater played an important role in reducing E. coli K12. Bacteriophage propagated in filtered influent or activated sludge when E. coli K12 was introduced, but not in raw influent or activated sludge. The methodology developed in this study can be applied in the actual environmental water to trace the fate of pathogens.
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Dreyfus, Marc, and Valérie Heurgué-Hamard. "Termination troubles in Escherichia coli K12." Molecular Microbiology 79, no. 2 (November 29, 2010): 288–91. http://dx.doi.org/10.1111/j.1365-2958.2010.07468.x.

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Дисертації з теми "Anaerobe E.coli K12"

1

Chaudhary, Nida. "Anaerobic fermentation of glycerol by Escherichia coli K12 for the production of ethanol." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:8881/R/?func=dbin-jump-full&object_id=92278.

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2

Simonte, Francesca Maria [Verfasser], and J. [Akademischer Betreuer] Gescher. "Untersuchung zur anaeroben Regulation des prp-Operons in Escherichia coli K12 im Hinblick auf die Entwicklung eines Propionatbiosensors / Francesca Maria Simonte ; Betreuer: J. Gescher." Karlsruhe : KIT-Bibliothek, 2016. http://d-nb.info/111427335X/34.

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3

Malik, Noor-e.-Mobeen. "Homologous intermolecular recombination in Escherichia coli K12." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621901.

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4

Hough, Melinda Terace. "Streptomycin and Escherichia coli K12 MG1655 cell death." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/24714.

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To investigate the molecular changes induced by streptomycin, spotted cDNA microarrays representing 99.6% of the E. coli K12 MG1655 genome were employed. Changes to the transcriptome were detected within 10-minutes of drug addition. Following 30-minutes of treatment, 172 transcripts representing 4% of the genome were differentially regulated; although rpsL, the molecular target of streptomycin, was not affected. A striking, and almost complete, induction of three key stress responses were observed: heat shock, phage shock, and drug sensitivity. Members of the heat shock response were most enriched; hsIS was consistently the highest up-regulated transcript. The most significant of the few uncharacterized transcripts observed was yccV, a hemi-methylated oriC DNA-binding protein. Surprisingly, a lack of coordination between the induction of the anaerobic metabolism transcriptional regulator, fnr, and its downstream targets was observed. Transcripts associated with the energy-rich processes of motility, chemotaxis, and anaerobic metabolism were repressed. To determine whether hsIS or yccV played a critical role in streptomycin-induced cell death, unmarked knock-out mutants were engineered and characterized. During mutant engineering, a previously undescribed large scar sequence was identified and sequenced. Neither mutant exhibited a reduction in fitness, change in the minimum inhibitory concentration, or dose-response when compared to the parent strain. These two targets may be important in the overall mechanism of streptomycin lethality, but are not solely responsible for it. The work presented herein provides a detailed picture of the immediate molecular response of E. coli K12 MG1655 to streptomycin and suggests that its antibiotic action as a whole is more complex than the interaction of a single compound with a single target.
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5

Brown, Angela. "An ecotoxicogenomic study in Escherichia coli K12-MG1655." Thesis, University of Newcastle Upon Tyne, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.417436.

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6

Picksley, S. M. "Inducible recombination and DNA repair in Escherichia coli K12." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.332729.

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Hagan, Nicola Frances Petrina. "Analysis of the Escherichia coli K12 RuvC recombination protein." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321461.

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8

PEBORDE, JEAN PASCAL. "Etude biochimique et immunologique du lipopolysaccharide d'escherichia coli k12." Toulouse 3, 1989. http://www.theses.fr/1989TOU30074.

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Detection du lipopolyoside (lps) au cours de la purification de proteines issues d'escherichia coli. Mise au point d'une technique elisa par inhibition, application au controle de la purification de l'hormone de croissance humaine (hgh) produite par e. Coli. Comportement chromatographique du lps sur differents supports et au cours de la purification de l'hgh. Etude des interactions lps/proteines dans le cas de la serumalbumine et de l'hgh
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9

Clugston, C. K. "Amplification of an IS1-flanked unit in Escherichia coli K12." Thesis, University of Glasgow, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.234833.

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10

Iobbi-Nivol, Chantal. "Mise en évidence d'une seconde nitrate réductase chez Escherichia coli K12." Aix-Marseille 2, 1989. http://www.theses.fr/1989AIX22069.

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Nous avons mis en evidence chez e. Coli l'existence d'une seconde nitrate reductase, appelee nitrate reductase z. Une etude biochnimique, montre que si cette enzyme presente de nombreuses proprietes similaire's a celles de la nitrate reductase a, leur sensibilite a la trypsine et leur comportement electrophoretique sur gel de polyacrylamide en conditions non denaturantes les differencient nettement. Une etude immunologique a mis en evidence l'existence d'epitopes communs et distincts entre elles. De plus, cette etude montre que la biosynthese des deux complexes enzymatiques est regulee de facon differente: une des fonctions de la nitrate reductase z serait d'assurer le transfert des electrons vers le nitrate lors de la transition aerobiose-anaerobiose. Les genes de structure des deux nitrates reductases sont tres homologues, identiques en taille et organisees de facon similaire. Leurs regions regulatrices sont par contre differentes. L'etude des sequences du gene narh de l'operon narghji, nous a conduits a reconsiderer le mecanisme fonctionnel de la nitrate reductase a. Chaque sous-unite de la nitrate reductase z semble posseder la meme fonction que la sous-unite qui lui est homologue dans le nitrate reductase a. A l'issu de ce travail, nous proposons que les operons narghji et parzywv proviennent d'une duplication de genes ancestraux qui aurait ete suivie d'une evolution divergente
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Книги з теми "Anaerobe E.coli K12"

1

Peakman, Timothy Charles. The regulation of the promoters of two genes involved in the NaDh-dependent nitrite reduction reaction in anaerobic cultures of Escherichia Coli K12. Birmingham: University of Birmingham, 1988.

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2

Macdonald, Heather. The molecular cloning of genes involved in nitrite reduction by 'Escherichia coli' K12. Birmingham: University of Birmingham, 1985.

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Частини книг з теми "Anaerobe E.coli K12"

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de Vries, Patrick, Colin G. Johnson, and Ian C. Blomfield. "Modelling fim Expression in Escherichia Coli K12." In Communications in Computer and Information Science, 14–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-16750-8_2.

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2

Di Lallo, Gustavo, Patrizia Ghelardini, and Luciano Paolozzi. "Two-hybrid Assay in Escherichia coli K12." In Prokaryotic Genomics, 194–203. Basel: Birkhäuser Basel, 2003. http://dx.doi.org/10.1007/978-3-0348-8963-6_15.

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3

Gershanovitch, V. N., and T. A. Holzmayer. "Amplification of the Adenylate Cyclase Gene in Escherichia Coli K12." In Gene Manipulation and Expression, 305–17. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-011-6565-5_22.

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4

Moon, Sanghoon, Yanga Byun, and Kyungsook Han. "Prediction of Ribosomal -1 Frameshifts in the Escherichia coli K12 Genome." In Computational Intelligence and Bioinformatics, 612–21. Berlin, Heidelberg: Springer Berlin Heidelberg, 2006. http://dx.doi.org/10.1007/11816102_65.

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5

Palermo, Deborah A., and Virginia L. Clark. "Characterization of Neisseria gonorrhoeae lipopolysaccharide biosynthesis genes cloned in Escherichia coli K12." In Gonococci and Meningococci, 575–80. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-009-1383-7_90.

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Hengge-Aronis, Regine. "The Role of rpoS in Early Stationary-Phase Gene Regulation in Escherichia coli K12." In Starvation in Bacteria, 171–200. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4899-2439-1_8.

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del Cardayre, Stephen, and J. B. Neilands. "Structure-Activity Correlations for the Ferric Uptake Regulation (FUR) Repressor Protein of Escherichia Coli K12." In Iron Biominerals, 387–96. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3810-3_28.

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8

Sørensen, Søren Johannes. "Plasmid Transfer of pBR322 Derivatives from recA’E. Coli K12 Donor Strain to Various Natural Gram-Negative Isolates." In The Release of Genetically Modified Microorganisms—REGEM 2, 197–98. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4613-0493-7_34.

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9

Savic, Dragutin J., Mila Jankovic, Tatjana Kostic, and Barry W. Glickman. "Molecular Basis of Mutagenesis in Escherichia Coli K12 Deficient for DNA Polymerase I: Special Role of the Gtcg Sequence." In Anticarcinogenesis and Radiation Protection 2, 57–60. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3850-9_8.

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10

De Feyter, Robert. "[45] Shikimate kinases from Escherichia coli K12." In Methods in Enzymology, 355–61. Elsevier, 1987. http://dx.doi.org/10.1016/s0076-6879(87)42047-8.

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Тези доповідей конференцій з теми "Anaerobe E.coli K12"

1

Ch-Th, Thomas, K. T. Drisya, M. Solis-Lopez, A. Romero-Nunez, and S. Velumani. "GO/BiVO4 NANOCOMPOSITES FOR Escherichia coli K12 PHOTOCATALYTIC INACTIVATION." In 2020 17th International Conference on Electrical Engineering, Computing Science and Automatic Control (CCE). IEEE, 2020. http://dx.doi.org/10.1109/cce50788.2020.9299170.

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2

McGlennen, Matthew, Markus Dieser, Christine Foreman, and Stephan Warnat. "Effects of Escherichia coli K12 Biofilm on Sensor Thin Film Materials." In 2019 IEEE SENSORS. IEEE, 2019. http://dx.doi.org/10.1109/sensors43011.2019.8956741.

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3

Song, Zhiqing, and Yunzhang Liang. "Extremely Low Frequency Electric Field Induced Mutations in Escherichia Coli K12 Strains." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5514939.

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4

Belda-Galbis, C. M., A. Martínez, and D. Rodrigo. "Antimicrobial effect of carvacrol on Escherichia coli K12 growth at different temperatures." In Proceedings of the International Conference on Antimicrobial Research (ICAR2010). WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814354868_0015.

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5

Lieyu, Zhang, Ye Yu-Bing, Xi Beidou, Wang Lei, Liu Tian, Yu Pengfei, and Lv Jingjing. "Research about the Removal Effect of the Constructed Wetland System on the Escherichia Coli K12." In 2015 AASRI International Conference on Circuits and Systems (CAS 2015). Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/cas-15.2015.16.

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6

Katherine L Bialka, Ali Demirci, Paul Walker, and Virendra M Puri. "Pulsed UV-light penetration characterization and the inactivation of Escherichia coli K12 in model systems." In 2007 Minneapolis, Minnesota, June 17-20, 2007. St. Joseph, MI: American Society of Agricultural and Biological Engineers, 2007. http://dx.doi.org/10.13031/2013.23299.

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Perni, S., G. Shama, P. R. Chalise, and M. Kong. "Nano-second pulse electric field has both a lethal and a sub-lethal effect on E. coli K12." In The 33rd IEEE International Conference on Plasma Science, 2006. ICOPS 2006. IEEE Conference Record - Abstracts. IEEE, 2006. http://dx.doi.org/10.1109/plasma.2006.1707304.

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