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Статті в журналах з теми "Amyloliquefaciens"
Zheng, Yangyang, Xudong Wang, Siyuan Liu, Kewei Zhang, Zhibo Cai, Xiuling Chen, Yao Zhang, Jiayin Liu, and Aoxue Wang. "The Endochitinase of Clonostachysrosea Expression in Bacillus amyloliquefaciens Enhances the Botrytis cinerea Resistance of Tomato." International Journal of Molecular Sciences 19, no. 8 (July 30, 2018): 2221. http://dx.doi.org/10.3390/ijms19082221.
Повний текст джерелаAhsan, Taswar, Chaoqun Zang, Shuyi Yu, Xue Pei, Jinhui Xie, Ying Lin, Xiaozhou Liu, and Chunhao Liang. "Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from Bacillus amyloliquefaciens, for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut (Arachis hypogaea L.)." Journal of Fungi 8, no. 11 (November 20, 2022): 1223. http://dx.doi.org/10.3390/jof8111223.
Повний текст джерелаRanjith, Sellappan, Thangavel Kalaiselvi, Muruganagounder Muthusami, and Uthandi Sivakumar. "Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (Spodoptera frugiperda) in Maize." Microorganisms 10, no. 9 (September 16, 2022): 1850. http://dx.doi.org/10.3390/microorganisms10091850.
Повний текст джерелаFang, Haitian, Huiyan Liu, Ning Chen, Chenglin Zhang, Xixian Xie, and Qingyang Xu. "Site-directed mutagenesis studies on the uridine monophosphate binding sites of feedback inhibition in carbamoyl phosphate synthetase and effects on cytidine production by Bacillus amyloliquefaciens." Canadian Journal of Microbiology 59, no. 6 (June 2013): 374–79. http://dx.doi.org/10.1139/cjm-2012-0758.
Повний текст джерелаDunlap, Christopher A., Soo-Jin Kim, Soon-Wo Kwon, and Alejandro P. Rooney. "Phylogenomic analysis shows that Bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of Bacillus methylotrophicus." International Journal of Systematic and Evolutionary Microbiology 65, Pt_7 (July 1, 2015): 2104–9. http://dx.doi.org/10.1099/ijs.0.000226.
Повний текст джерелаNan, Jing, Shaoran Zhang, and Ling Jiang. "Antibacterial Potential of Bacillus amyloliquefaciens GJ1 against Citrus Huanglongbing." Plants 10, no. 2 (January 29, 2021): 261. http://dx.doi.org/10.3390/plants10020261.
Повний текст джерелаWang, Da-Cheng, Chun-Hao Jiang, Li-Na Zhang, Lin Chen, Xiao-Yun Zhang, and Jian-Hua Guo. "Biofilms Positively Contribute to Bacillus amyloliquefaciens 54-induced Drought Tolerance in Tomato Plants." International Journal of Molecular Sciences 20, no. 24 (December 12, 2019): 6271. http://dx.doi.org/10.3390/ijms20246271.
Повний текст джерелаWei, Xubiao, Xiudong Liao, Jun Cai, Zhaojun Zheng, Lulu Zhang, Tingting Shang, Yu Fu, Cong Hu, Lei Ma, and Rijun Zhang. "Effects of Bacillus amyloliquefaciens LFB112 in the diet on growth of broilers and on the quality and fatty acid composition of broiler meat." Animal Production Science 57, no. 9 (2017): 1899. http://dx.doi.org/10.1071/an16119.
Повний текст джерелаDomenico Prisa. "Antifungal and plant growth promoting activity with Bacillus amyloliquefaciens in Aeonium subs." Magna Scientia Advanced Biology and Pharmacy 1, no. 2 (February 28, 2021): 042–50. http://dx.doi.org/10.30574/msabp.2021.1.2.0008.
Повний текст джерелаPasaribu, T., E. B. Laconi, and I. P. Kompiang. "Evaluation of the nutrient contents of palm kernel cake fermented by microbial cocktails as a potential feedstuff for poultry." Journal of the Indonesian Tropical Animal Agriculture 44, no. 3 (September 24, 2019): 295. http://dx.doi.org/10.14710/jitaa.44.3.295-302.
Повний текст джерелаДисертації з теми "Amyloliquefaciens"
Makarewicz, Oliwia. "Regulation der Phytaseexpression in Bacillus amyloliquefaciens." Doctoral thesis, [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=983031339.
Повний текст джерелаLima, Frederico Alves. "Produção de biossurfactantes por Bacillus amyloliquefaciens IT45." Universidade Federal de Uberlândia, 2017. http://dx.doi.org/10.14393/ufu.di.2017.62.
Повний текст джерелаBiossurfactantes são moléculas de origem microbiana que possuem importante ação na redução tensão superficial. Dentre os biossurfactantes mais efetivos estão os lipopeptídeos produzidos por bactérias do gênero Bacillus, especialmente a Surfactina. Estes biocompostos apresentam uma série de vantagens que potencializam suas aplicações, tais como: estabilidade frente a condições extremas (pH, temperatura), diversidade de estruturas químicas, excelentes propriedades superficiais e ecológicas, ação antibiótica frente a microrganismos patógenos, dentre outras. Diante deste contexto, neste trabalho foi avaliada a produção de biossurfactantes totais e a Surfactina por Bacillus amyloliquefaciens IT45, quando variada a concentração dos reagentes presentes no meio de cultivo. As fermentações submersas foram realizadas em mesa agitadora industrial e em biorreator piloto de capacidade 40 litros. Para aperfeiçoar a concentração dos reagentes presentes no meio de cultura, um Planejamento Composto Central foi desenvolvido com propósito de avaliar a influência de três variáveis (xarope de glicose, extrato de levedura e cloreto de cálcio) na tensão superficial, na produção de biossurfactantes totais e no açúcar residual. Depois das análises estatísticas, quando as variáveis estavam nas concentrações (g.L-1) de 20 para xarope de glicose, 15 para extrato de levedura e 4 para cloreto de cálcio, a tensão superficial (mN/m) foi reduzida de valores acima de 50 para 30, o açúcar residual foi mínimo e igual a 31% e a produção de biossurfactantes totais foi máxima e igual a 5,5 g.L-1, depois de um período de cultivo de 48 horas. A caracterização do biossurfactante sugeriu a presença da Surfactina e este composto foi quantificado no tempo de retenção de 13,5 minutos. Com intuito de saber a real produção de Surfactina e crescimento biomassa celular, foram feitas fermentações em biorreator piloto de 40 litros e os resultados mostraram bastantes favoráveis. O tratamento com maior destaque foi referente à receita sugerida pelo Planejamento Composto Central em que o xarope de glicose, extrato de levedura e cloreto de cálcio estavam nas concentrações (g.L-1) de 20, 15 e 4, respectivamente. Neste cultivo o crescimento celular de 6,0 x 109 CFU.mL-1, produção de Surfactina de 0,63 g.L-1 e açúcar residual de 28%. Também foi realizado teste de atividade antimicrobiana contra 5 fungos patogênicos de diferentes gêneros. O caldo fermentado livre de células mostrou-se promissor, pois causou inibição em 4 fungos dos 5 estudos. Portanto, os resultados demonstram que o Bacillus amyloliquefaciens IT45 tem potencial para produção de biocompostos, uma vez que não necessita de altas concentrações de fonte de carbono e nitrogênio para seu desenvolvimento.
Biosurfactants are molecules of microbial origin that have superficial action. Among the most effective are the lipopeptide biosurfactants produced by Bacillus, especially surfactin. These biological products have a number of advantages that potentiate their applications, such as: stability to extreme conditions (pH, temperature), diversity of chemical structures, excellent surface and ecological properties, antibiotic action against pathogenic microorganisms, etc. In this context, the productions of total biosurfactants and Surfactin by Bacillus amyloliquefaciens IT45 were evaluated when the reagents concentration present in the culture medium varied. The submerged fermentations were carried out in an industrial shaker and in a pilot bioreactor of 40 liters capacity. In order to improve the reactants concentration, a Central Composite Design was developed to evaluate the influence of three variables (glucose syrup – Glucodry, yeast extract and calcium chloride) on superficial tension, total biosurfactant production and residual sugar. After statistical analyzes, when the variables were in the concentrations (g.L-1) of 20 for Glucodry, 15 for yeast extract and 4 for calcium chloride, the superficial tension (mN/m) reduces values above 50 to about 30, the residual sugar was minimal, around 31% and the total biosurfactant production was maximum, around 5.5 gL-1, after a period of 48 hours. The characterization of the biosurfactant identified Surfactin presence that was quantified in the retention time of 13.5 minutes. In order to know the real production of Surfactin and cellular biomass growth, fermentations were made in a 40 liter pilot bioreactor and the results were quite favorable. The most important culture medium suggested by the Central Composite Design, where glucose syrup, yeast extract and calcium chloride were in the concentrations (g.L-1) of 20, 15 and 4, respectively. For this fermentation, the cellular growth was 6.0 x 109 CFU.mL-1, Surfactin production was 0.63 g.L-1 and residual sugar was 28%. The results demonstrate that Bacillus amyloliquefaciens IT45 has potential to produce biosurfactants and does not require high concentrations of carbon and nitrogen sources for its development.
Dissertação (Mestrado)
Chu, Hoang Ha. "Identification and functions of type I signal peptidases of Bacillus amyloliquefaciens." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=964358999.
Повний текст джерелаBashir, Abdallah. "Charakterisierung der Rolle von genereller Stressantwort und Sporulation bei der Anpassung von Bacillus-Stämmen an den Standort Boden." [S.l. : s.n.], 2004. http://archiv.ub.uni-marburg.de/diss/z2004/0512/.
Повний текст джерелаMattapally, Peter Vijay. "Characterizing Bacillus amyloliquefaciens UCMB5113 on a Plant Model Arabidopsis thaliana." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-38378.
Повний текст джерелаBonelli, Raquel Regina. "Extração e caracterização de uma substância antimicrobiana produzida por bacillus amyloliquefaciens." Florianópolis, SC, 2001. http://repositorio.ufsc.br/xmlui/handle/123456789/80287.
Повний текст джерелаMade available in DSpace on 2012-10-18T12:26:38Z (GMT). No. of bitstreams: 0
Extraiu-se uma substância antimicrobiana de uma cultura 18-24 h de Bacillus amyloliquefaciens em Caldo Triptona de Soja suplementado com Extrato de Levedura a 0,6% (TSB-YE - OXOID) por precipitação com 20% de saturação de sulfato de amônio, ressuspensão em tampão fosfato pH 7,2, diálise em membrana 3,5kD (Spectra/Por) e esterilização por filtração (membrana 0,22mm - Millipore). Esta substância foi caracterizada segundo sua sensibilidade a temperatura (50, 75 e 100°C) e pH (2,0 a 9,0). Para determinação de sua natureza química empregaram-se as enzimas (Sigma) protease de S. griseus (P6911), protease de A. saitoi (P2143), a-quimotripsina (C4129) e pepsina (P6887) a 50mg/mL e a a-amilase Termamyl 120 L (Novo Nordisk) a 1% v/v. Para verificação da manutenção da atividade após tais ensaios, o extrato foi aplicado em poços preparados em placas de Ágar Triptona de Soja suplementado com Extrato de Levedura a 0,6% (TSA-YE - OXOID) pré-semeadas com 0,1 mL de uma cultura 105 UFC/mL de Listeria monocytogenes. Determinou-se sua concentração mínima inibitória (CMI) sobre uma cultura 104 UFC/mL de L. monocytogenes e seu peso molecular por eletroforese em gel de poliacrilamida (SDS-PAGE). Como resultado dos testes de caracterização, a substância apresentou-se estável a 50°C, perdendo sua atividade gradualmente a 75°C e rapidamente a 100°C. Também foi estável em valores de pH de 2,0 a 9,0. Perdeu parcialmente sua atividade quando tratada com as enzimas protease de S.griseus, protease de A. saitoi e a-amilase, indicando uma estrutura glicoproteica. A CMI foi 25% v/v e a substância agiu de forma bacteriostática. A eletroforese indicou um peso molecular inferior a 6.500 Daltons.
Schulz, Denys. "Avaliação toxicológica do extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10." Florianópolis, SC, 2008. http://repositorio.ufsc.br/xmlui/handle/123456789/91977.
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Alguns microrganismos possuem a capacidade de produzir substâncias que podem influenciar no desenvolvimento de outros microrganismos. Desde os anos 50 é relatada a capacidade de várias espécies de bactérias do gênero Bacillus de produzir substâncias com atividade antimicrobiana como peptídeos, também denominados de bacteriocinas e enzimas como a subtilisina, subtilina, as proteases e as termolisinas. Muitos desses peptídeos e enzimas têm sido caracterizados bioquimicamente e geneticamente. Embora sejam conhecidas, a função estrutural, a biossíntese e modo de ação de alguns peptídeos antimicrobianos e enzimas, muitos aspectos desses compostos ainda permanecem desconhecidos. Frente a essa realidade, o presente estudo investigou a atividade antibacteriana e antifúngica e a toxicidade in vitro e in vivo do extrato bruto de Bacillus amyloliquefaciens R 10. O extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 foi obtido por técnicas de precipitação de proteínas, centrifugação, diálise e esterilização por filtração. A padronização das análises toxicológicas e de atividade antibacteriana e antifúngica foi realizada pela determinação da concentração de proteínas do extrato bruto de Bacillus amyloliquefaciens R 10. O ensaio de atividade antibacteriana e antifúngica foi realizado pela técnica de difusão em poços. Dentre os diferentes indicadores utilizaram-se bactérias Gram-positivas (Listeria monocytogenes NCTC 098630, Staphylococcus aureus ATCC 25923 e Enterococcus faecalis ATCC 29212), Gram-negativas (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048 e Pseudomonas aeruginosa ATCC 9027), fungos (Aspergillus fumigatus ATCC 9197 e Penicillium commune J 238) e leveduras (Rhodotorula muscilaginosa J 350, Pichia anomala DSM 70255 e Kluveromyces marxianus ATCC 16045). O extrato bruto foi testado nas concentrações de 5, 20, 40, 60 e 80 g de proteínas, em ambos os ensaios de atividade antimicrobiana. Para avaliar a toxicidade do extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 foram utilizados ensaios in vitro (genotoxicidade) e in vivo (toxicidade aguda em ratos e camundongos e toxicidade de doses repetidas em camundongos). A investigação da genotoxicidade com células VERO ATCC-CCL 81 foi realizada pelo ensaio Cometa, utilizando-se 60, 80 e 100 g de proteínas do extrato bruto. Para análise toxicológica aguda em ratos Wistar (Rattus norvegicus), utilizou-se um grupo de 10 animais (5 machos e 5 fêmeas) tratado com o extrato bruto, por gavagem oral, com a dose de 2.000µg/kg (dose máxima recomendada). Os animais foram pesados no início e no final do experimento e observados por 14 dias quanto aos possíveis sinais de toxicidade (morte, coma, convulsão, prostração, ataxia, tremores, alteração na pele ou pêlo, alteração nas mucosas, diarréia e salivação). Para análise toxicológica aguda em camundongos Swiss (Mus musculus), utilizou-se o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 por gavagem oral, numa única administração, nas doses de 5, 50, 500 e 5.000 g/kg a 80 camundongos (40 machos e 40 fêmeas, contendo 10 animais por grupo). Outros 20 camundongos (10 machos e 10 fêmeas) receberam veículo (salina, NaCl 0,9%), pela mesma via. Para análise toxicológica de doses repetidas em camundongos Swiss (Mus musculus) utilizou-se o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 administrado por gavagem oral, por um período de 90 dias nas doses de 50, 500 e 5.000 g/kg em 120 animais (60 machos e 60 fêmeas, contendo 20 animais por grupo). Outros 40 camundongos (20 machos e 20 fêmeas) receberam veículo (salina, NaCl 0,9%), pela mesma via. Constatou-se nos ensaios de atividade antimicrobiana que o extrato bruto de Bacillus amyloliquefaciens R 10 não apresentou atividade contra os bolores e as leveduras nas concentrações testadas, e somente apresentou atividade antibacteriana frente Listeria monocytogenes NCTC 098630. O extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 não foi genotóxico para células VERO na concentração de 60µg/ml, porém apresentou genotoxicidade nas concentrações de 80 e 100µg/ml. Na análise toxicológica aguda em ratos a dose letal 50 (DL50) foi superior a 2.000µg/kg. Na análise toxicológica aguda em camundongos, o extrato bruto apresentou boa tolerabilidade e reduzidos efeitos tóxicos agudos importantes, com DL50 superior a 5.000µg/kg. Já na análise toxicológica de doses repetidas, a non toxic adverse effect level (NOAEL) para o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 situa-se entre as doses de 50 e 500 g/kg. Os resultados indicam que o extrato bruto antibacteriano de Bacillus amyloliquefaciens R 10 apresenta um grande potencial como conservador natural de alimentos, dada sua marcante atividade antilisterial e sua relativa segurança toxicológica. Some microorganisms are able to produce substances that can influence the growth of other microorganisms. The ability of various bacterial species of the genus Bacillus to produce substances with antimicrobial activity such as peptides, also called bacteriocins, and enzymes such as subtilisin, subtilin, proteases and thermolysins has been reported since the 1950's. Many of these peptides and enzymes have been characterized biochemically and genetically. Although the structural function, biosynthesis and mode of action of some antimicrobial peptides and enzymes have been identified, many aspects of these compounds are still unknown. The present study investigated the antibacterial and antifungal activity and the in vitro and in vivo toxicity of the crude extract of Bacillus amyloliquefaciens R 10. The antibacterial crude extract of Bacillus amyloliquefaciens R 10 was obtained by techniques of protein precipitation, centrifugation, dialysis and sterilization through filtration. The standardization of the toxicological analysis, and antibacterial and antifungal activity was realized by determining the concentration of proteins in the crude extract of Bacillus amyloliquefaciens R 10. The assay of antibacterial and antifungal activity was done by agar diffusion technique in wells. Among the different indicators were used the Gram-positive bacteria (Listeria monocytogenes NCTC 098630, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048 and Pseudomonas aeruginosa ATCC 9027), molds (Aspergillus fumigatus ATCC 9197 and Penicillium commune J 238), and yeasts (Rhodotorula muscilaginosa J 350, Pichia anomala DSM 70255 and Kluveromyces marxianus ATCC 16045). The crude extract was tested in concentrations of 5, 20, 40, 60 and 80 g of protein in both assays of antimicrobial activity. To evaluate the Bacillus amyloliquefaciens R 10 antibacterial crude extract's toxicity were used in vitro (genotoxicity) and in vivo (acute toxicity in rats and mice and repeated doses toxicity in mice) assays. The research of the genotoxicity with VERO ATCC-CCL 81 cells was made by Comet assay, using 60, 80 e 100 g of protein of the crude extract. To the acute toxicological analysis in rats Wistar (Rattus norvegicus), were used one group with 10 animals (5 males and 5 females) treated with the crude extract by oral gavage, with dose of 2,000µg/kg (maximum dose recommended). The animals were weighed at the beginning and at the end of the experiment, and observed during 14 days as the possible toxicity signs (death, coma, convulsions, prostration, ataxy, tremors, skin or hair alterations, mucous alteration, diarrhea and salivation). To the acute toxicological analysis in Swiss mice (Mus musculus), the antibacterial crude extract of Bacillus amyloliquefaciens R 10 was used by oral gavage, in a single administration, in doses of 5, 50, 500 and 5,000 g/kg to 80 mice (40 males and 40 females, with 10 animals per group). Other 20 mices (10 males and 10 females) received the vehicle (saline, NaCl 0.9%), through the same route. To the repeated doses toxicological analysis in Swiss mice (Mus musculus) the antibacterial crude extract of Bacillus amyloliquefaciens R 10 was administrated by oral gavage during 90 days in doses of 50, 500 and 5,000 g/kg in 120 animals (60 males and 60 females, in groups of 20 animals). Other 40 mice (20 males and 20 females) received the vehicle (saline, NaCl 0.9%), through the same route. It was verified in the antimicrobial activity assays that the crude extract of Bacillus amyloliquefaciens R 10 didn't present activity against molds and yeasts in the tested concentrations, and only presented antibacterial activity against Listeria monocytogenes NCTC 098630. The antibacterial crude extract of Bacillus amyloliquefaciens R 10 was not genotoxic to VERO cells at concentration of 60µg/ml, but showed genotoxicity at concentration of 80 and 100µg/ml. In the acute toxicological analysis in rats the letal dose 50 (DL50) was higher than 2,000µg/kg. In the acute toxicological analysis in mice, the crude extract presented good tolerability and litle important acute toxic effects with DL50 higher than 5,000µg/kg. In the repeated doses toxicological analysis, a non toxic adverse effect level (NOAEL) for the antibacterial crude extract is between the doses of 50 and 500 g/kg. The results showed that the antibacterial crude extract of Bacillus amyloliquefaciens R 10 presents a great potential as a natural food preservative, due to its notable antilisterial activity, and its relative toxicological security.
Scholz, Romy. "Synthese der Bacteriocine Amylocyclicin A und Plantazolicin in Bacillus amyloliquefaciens FZB42." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://dx.doi.org/10.18452/16283.
Повний текст джерелаBacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. Five gene clusters direct the non-ribosomal synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide and the iron-siderophore bacillibactin. Three gene clusters direct the non-ribosomal synthesis of the antibacterial acting polyketides macrolactin, bacillaene and difficidin; in addition to the non-ribosomal synthesis of the antibacterial dipeptide bacilysin. Genes involved in ribosome-dependent synthesis of lantibiotics and other peptides are scarce. Only two incomplete gene clusters directing immunity against mersacidin and subtilin were found. In this work two ribosomally synthesized antibacterial peptides, amylocyclicin A and plantazolicin, and their corresponding gene clusters were identified. Amylocyclicin A is a circular peptide with a mass of 6381 Da and strong activity against Gram-positive bacteria. Six genes are responsible for the synthesis, maturation, export and immunity of this peptide belonging to group I of circular bacteriocins. Plantazolicin is a strongly modified hydrophobic peptide bearing a molecular mass of 1,335 Da and displaying antibacterial activity toward closely related Gram-positive bacteria. Essential modification contains the incorporation of azole heterocycles, which derive from Cys, Ser, and Thr residues of the precursor peptide and addition of two methyl groups. Twelve genes are responsible for synthesis, modification, export and immunity of this peptide belonging to the TOMM group of thiazol/oxazol modified microcins.
Benitez, Lisianne Brittes. "Caracterização de peptídeos antimicrobianos de Bacillus amyloliquefaciens com atividade antibacteriana, antifúngica e amebicida." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2010. http://hdl.handle.net/10183/29950.
Повний текст джерелаBacteriocins are small peptides synthesized by bacteria which have antimicrobiall properties. This study aimed to identify and characterize a bacteriocin produced by Bacillus amyloliquefaciens LBM 5006, isolated from soil of Mata Atlântica, Santa Catarina, Brazil. It has been observed that the antimicrobial substance production starting at the exponencial grown phase and maximum activity occur at stationary phase. The effect of different bacteria on the production of antimicrobial activity by Bacillus amyloliquefaciens LBM 5006 was investigate. It was concluded that the presence of intact or thermally inactivated cells of Escherichia coli enhanced the synthesis of antimicrobial peptides by B. amyloliquefaciens strain. Bacteriocin LBM 5006 showed broad spectrum of action, producing antagonistic activity against bacteria, fungi and pathogenic amoebas. The mode of action against Listeria monocytogenes and Paenibacillus larvae was bactericidal and bacteriolytic. Sporicidal activity was observed against P. larvae spores after treatment whit 1600 AU mL-1. Amoebicidal and amoebistatic effects were detected against throphozoites of Acanthamoeba poliphaga and cell lysis. The bacteriocin had no inhibitory effect on Vero cells at concentrations that were effective against amoebas.The antimicrobial substance was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction.The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s) in its structure. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides, with main peaks at m/z 1058 Da and 1464 Da, corresponding to iturinlike and fengycin-like peptides, respectively.
Larini, Mariana Munhoz. "Produção de surfactina por Bacillus amyloliquefaciens MO.04b em mistura de resíduos agroindustriais." Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2017. http://www.bibliotecadigital.uel.br/document/?code=vtls000216058.
Повний текст джерелаBrazil, important in the world's agricultural scenario, is a producer of soybeans, high-protein grain, sugar cane and rice. These cultures, when processed, generate materials that are potential substrates for the microbial culture and production of metabolites of high added value. A solid state fermentation (FES) allows the cultivation of microorganisms in a condition of low moisture content and favors the use of different agroindustrial residues. The objective of this work is to evaluate the production of surfactin, a lipopeptide biosurfactant, by FES using Bacillus amyloliquefaciens MO.04b. The microorganism was cultivated in a mixture of agroindustrial residues containing soybean meal, sugarcane bagasse and rice husk, moistened with salt solution plus 0.2% glucose (m/v), inoculated with cell suspension (107-108 CFU ml-1) and incubated at 37 ± 2 °C. After interruption of FES with distilled water, the mixture was homogenized, the biomass determined by turbidimetry at 600 nm and counting of the colony forming units (CFU). This mixture was subjected to centrifugation at 3500 x g for 15 min. The weight of the fermented solid material was determined by gravimetry and the free cell extract (ELC) used for the determination of the pH (initial and final), activity of enzymes protease, xylanases, cellulases and laccases, total proteins, total sugars and reduced sugar. The lipids contained in the ELC were precipitated with addition of 6 mol L-1 HCl to pH 2.0 and held for 12 hours and extracted with a mixture of chloroform and methanol [CHCl3:CH3OH (4:1)]. The obtained material was then lyophilized. The quantification of surfactin was performed by high performance liquid chromatography (HPLC). The final pH of the cultures was increased over the growing time and reached the maximum of 8.5 in 72 hours, as well as the biomass, which grew over time, up to 18 - 24 hours, reaching a maximum of 1 x 108 CFU mL-1. As regards the fermented solid material, a progressive reduction of dry weight was observed from 1.2207g (0 h) to 0.9268 g (72 h), showing that in 72 h of culture the microorganism hydrolyzed 25% of the total agroindustrial waste. The activity of proteases and xylanases were 0.946 U mL-1 and 0.81 U mL-1, respectively, between 6 and 12 hours of culture. No cellulase and laccase activities were detected. Surfactin was produced in all analyzed periods, and had a higher production in 18 hours of culture, of 64.15 mg mL-1. The mixture of residues used favored the growth of the microorganism, which was able to consume 25% of the residues under the FES conditions presented. These conditions allowed the bacterium to produce protease and xylanase and surfactin enzymes, whose highest production occurred in 18 hours of culture.
Книги з теми "Amyloliquefaciens"
Hewitt, Christopher Julian. The synthesis of alpha-amylase (E.C.3.2.1.1.) by Bacillus amyloliquefaciens B20. Birmingham: University of Birmingham, 1993.
Знайти повний текст джерелаHillier, Peter K. The effect of nutrient limitation on the cell growth and alpha-amylase(E.C.3.2.1.1): Production of Bacillus amyloliquefaciens B155 in continuous culture. Birmingham: University of Birmingham, 1996.
Знайти повний текст джерелаЧастини книг з теми "Amyloliquefaciens"
Bawden, M. J., T. Litjens, T. R. Hercus, B. K. May, and W. H. Elliott. "Extracellular Protease Production by B. Amyloliquefaciens." In Extracellular Enzymes of Microorganisms, 89–92. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4684-1274-1_11.
Повний текст джерелаVehmaanperä, Jari. "Bacillus amyloliquefaciens — Production Host for Industrial Enzymes." In Electrotransformation of Bacteria, 119–23. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-662-04305-9_14.
Повний текст джерелаWang, Depei, Kele Li, Yingying Wang, Ying Yang, and Jian Zhang. "Purification and Characterization of Antifungal Lipopeptide from Bacillus amyloliquefaciens BI2." In Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012), 465–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-37916-1_48.
Повний текст джерелаHamdache, Ahlem, Mohammed Ezziyyani, and Ahmed Lamarti. "Evaluation of Strawberry Seed Treatments with Biological Control Agents Bacillus amyloliquefaciens." In Advances in Intelligent Systems and Computing, 175–82. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-36664-3_20.
Повний текст джерелаBorriss, Rainer. "Phytostimulation and Biocontrol by the Plant-Associated Bacillus amyloliquefaciens FZB42: An Update." In Bacilli and Agrobiotechnology, 163–84. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-44409-3_8.
Повний текст джерелаAsraoui, Meryem, Filipo Zanella, Stefania Marcato, Andrea Squartini, Jamila Amzil, Ahlem Hamdache, Barbara Baldan, and Mohammed Ezziyyani. "Bacillus amyloliquefaciens Enhanced Strawberry Plants Defense Responses, upon Challenge with Botrytis cinerea." In Advances in Intelligent Systems and Computing, 46–53. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-11878-5_5.
Повний текст джерелаWang, Yajun, Zhanglei Cao, Miao Yu, and Depei Wang. "Application of Orthogonal Design to Optimize Fermentation Conditions of Bacillus amyloliquefaciens BI2." In Lecture Notes in Electrical Engineering, 487–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46318-5_51.
Повний текст джерелаSong, Ping, Guang-lun Lei, Xin Ma, Pei-jun Cai, and Cheng-cheng Chu. "Basic Research on the Application of Enzyme Breaker Produced by Bacillus Amyloliquefaciens." In Springer Series in Geomechanics and Geoengineering, 2857–63. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2485-1_263.
Повний текст джерелаBorriss, Rainer. "Phytostimulation and Biocontrol by the Plant-Associated Bacillus amyloliquefaciens FZB42: An Update." In Environmental and Microbial Biotechnology, 1–20. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-2576-6_1.
Повний текст джерелаBorriss, Rainer. "Comparative Analysis of the Complete Genome Sequence of the Plant Growth-Promoting Bacterium Bacillus amyloliquefaciens FZB42." In Molecular Microbial Ecology of the Rhizosphere, 883–98. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118297674.ch83.
Повний текст джерелаТези доповідей конференцій з теми "Amyloliquefaciens"
Tarakanov, R. I., P. A. Vasilyev, K. S. Troshin, and F. S. U. Dzhalilov. "Activity of Bacillus amyloliquefaciens MBI600 against soybean bacterioses." In Agrobiotechnology-2021. Publishing house of RGAU - MSHA, 2021. http://dx.doi.org/10.26897/978-5-9675-1855-3-2021-187.
Повний текст джерелаŠarmaitytė, Luka, Aušra Zigmontienė, and Domantas Tracevičius. "MAISTO ATLIEKŲ AEROBINIO FERMENTAVIMO PANAUDOJANT BAKTERIJAS (BACILLUS SUBTILIS, LACTOBACILLUS SALIVARIUS, BACILLUS AMYLOLIQUEFACIENS) IR JUODOSIOS PLOKŠČIAMUSĖS (HERMETIA ILLUCENS) LERVAS TYRIMAS." In 22-oji jaunųjų mokslininkų konferencijos „Mokslas – Lietuvos ateitis“ teminė konferencija APLINKOS APSAUGOS INŽINERIJA. Vilnius Gediminas Technical University, 2020. http://dx.doi.org/10.3846/aainz.2019.014.
Повний текст джерелаDepei Wang, Hui Meng, Ting Zhou, and Kele Li. "Study on fermentation conditions of antibacterial substance from Bacillus amyloliquefaciens BI2." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5965323.
Повний текст джерелаZhou, C., S. Xu, M. Zhang, Y. Chen, X. Guan, S. Wu, and Z. Bai. "Utilization of sweet potato starch wastewater for biofertilizer production byBacillus amyloliquefaciens." In SUSTAINABLE IRRIGATION 2012. Southampton, UK: WIT Press, 2012. http://dx.doi.org/10.2495/si120201.
Повний текст джерелаXu, Yun-Long, Xiang-Rong Liu, and Yao-Wen Zhang. "The biosolubilization of Shenfu coal by Bacillus amyloliquefaciens and Saccharothrix xinjiangensis." In 2017 6th International Conference on Energy and Environmental Protection (ICEEP 2017). Paris, France: Atlantis Press, 2017. http://dx.doi.org/10.2991/iceep-17.2017.83.
Повний текст джерелаLu, Hedong, Chengyuan Gu, Panping Yang, Hai Xu, Muhanmmad Bilal, and Yan Ding. "Optimization of Cr(VI) Adsorption by Bacillus amyloliquefaciens and Its Mechanism Study." In The International Conference on Biomedical Engineering and Bioinformatics. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011382700003443.
Повний текст джерелаLu, Hedong, Chengyuan Gu, Tao Yan, Qihan Zhang, Chengxin Geng, and Can Lv. "Screening and Identification of Bacillus amyloliquefaciens from the Gut of Aquatic Animals." In The International Conference on Biomedical Engineering and Bioinformatics. SCITEPRESS - Science and Technology Publications, 2022. http://dx.doi.org/10.5220/0011297900003443.
Повний текст джерелаWu, Fei, Xixian Xie, Jianming Shi, Qingyang Xu, and Ning Chen. "Molecular Cloning, Expression and Enzymatic Characterization of Inosine Monophosphate Dehydrogenase from Bacillus amyloliquefaciens." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5518002.
Повний текст джерелаZhao, Hailan, Yao Peng, Jing Xu, Haoming Xu, Wenqi Huang, Jiaqi Wang, Xue Guo, et al. "IDDF2021-ABS-0200 Bacillus amyloliquefaciens combined with resistant starch to ameliorate intestinal inflammation." In Abstracts of the International Digestive Disease Forum (IDDF), Hong Kong, 4–5 September 2021. BMJ Publishing Group Ltd and British Society of Gastroenterology, 2021. http://dx.doi.org/10.1136/gutjnl-2021-iddf.58.
Повний текст джерелаAbreu, Luciana De Paiva Santos, Carlos Eduardo De Souza Teodoro, and Ana Paula Martinazzo. "ATIVIDADE ANTIFÚNGICA IN VITRO DO SOBRENADANTE DA BACTÉRIA BACILLUS AMYLOLIQUEFACIENS SOBRE O FUNGO ASPERGILLUS FLAVUS." In II Congresso Brasileiro de Ciências Biológicas On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1300.
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