Дисертації з теми "Aliments – Teneur en sodium"
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Gobet, Mallory. "Etude par spectroscopies de RMN 23Na , 31P et 1H : effets de la teneur en sel (NaCl) dans des matrices alimentaires." Dijon, 2008. http://www.theses.fr/2008DIJOS063.
Повний текст джерелаThe reduction of salt (NaCl) content in food has become a matter of public health. However, the multiple functions of salt in food make the reduction of its content difficult. The aim of this work was to demonstrate the applicability of NMR innovative techniques in order to characterise the mobility of sodium ions (distinction between ‘free’ and ‘bound’ sodium), to bring a better understanding of the role of salt in the organisation of the food matrix (in particular phosphorous molecules in dairy systems) and to study impact of salt on the mobility of aroma compounds. In a first step, the 23Na NMR study of iota-carrageenan gels validated the quantification of total sodium (Single-Quantum, SQ experiment) and also demonstrated the involvement of ‘bound’ sodium ions in the gelation process of this polysaccharide (Double-Quantum Filtered, DQF experiment). The diffusion of aroma compounds in these systems was measured by 1H DOSY NMR, but no specific impact of salt was evidenced. The second step was dedicated to study dairy systems and semi-hard cheeses by 23Na NMR and solid-state 31P NMR using the magic angle spinning (MAS) technique. We demonstrated the feasibility of 23Na NMR methods for the quantification of total sodium and the characterisation of Na motional state (presence of ‘bound’ ions) in these systems. 31P MAS NMR (simple pulse excitation, SPE and cross-polarisation, CP) enabled the identification of different phosphorous compounds (inorganic phosphate, colloidal calcium phosphate, phosphoserins), the determination of their proportions and also permitted to determine the involvement of these phosphorous compounds in the protein network of coagulated systems. With these results, we achieved to identify the parameters of composition (or processing) influencing the dynamics of sodium ions in real cheeses. We also determined the distribution of phosphorous compounds, in particular inorganic phosphate, in these cheeses. This work demonstrates that these 23Na and 31P NMR innovative techniques are perfectly applicable to real food and can help to adjust the manufacturing process of cheeses in order to optimise their sensory and functional properties
Harfouch, Muhssen. "Etude expérimentale et modélisation des phénomènes de diffusion et de modification de texture des fromages de type Feta conservés en saumure." Vandoeuvre-les-Nancy, INPL, 1990. http://www.theses.fr/1990INPL090N.
Повний текст джерелаAl, Hajj Sally. "Effets des concentrations élevées en chlorure de sodium sur les fonctions immunitaires des cellules dendritiques." Electronic Thesis or Diss., Tours, 2019. http://www.theses.fr/2019TOUR3309.
Повний текст джерелаRecent evidence showed that in response to elevated sodium dietary intakes, many body tissues retain Na+ ions for long periods to reach concentrations up to 200 mM. Recent studies suggested that the immune system might be the bridge linking high sodium intake to several cardiovascular diseases and cancer progression as well. However, the studies about the effects of sodium on immunity brought about contrasted results. So far the effects of sodium on human dendritic cells (DCs) remain unknown. Considering their central role in the immune response, we tested how sodium chloride-enriched medium influences the immune properties of human DCs. DCs were derived from CD14+ monocytes from healthy donors and then stimulated by LPS, in sodium-enriched medium (from 140 up to 200 mM) and finally analyzed. We found that DCs cultivated in high Na+ concentrations remain viable and maintain the expression of DC markers up to 200 mM. In response to LPS, their maturation, their chemotaxis toward CCL19, their production of pro-inflammatory cytokines and ROS were inhibited by high [Na+]. In line with these results, we report that the T-cell allostimulatory capacity of DCs was also inhibited. Finally our data indicate that these effects were mediated through the phosphorylation of SGK-1 (serum and glucocorticoid induced kinase-1) and ERK1/2 kinases.Our results raised the possibility that the effects of sodium on T-cells might be counterbalanced by its ability to downregulate DC activation. Therefore the effects of high sodium diet on the immune response might be more complex than previously thought
Pelletier, Samuel-Claude. "La sensibilité gustative au sodium : un facteur clé dans la consommation de sel? : pistes pour la pratique infirmière chez les adultes." Master's thesis, Université Laval, 2020. http://hdl.handle.net/20.500.11794/66426.
Повний текст джерелаOver-consumption of salt is a worrying phenomenon on a global scale and is responsible for a growing number of health problems. Indeed, due to its close relationship with high blood pressure, over-consumption of salt is a major risk factor in the development of cardiovascular disease. It is therefore essential for the nurse to achieve a comprehensive understanding of salt consumption in order to develop effective nursing interventions in the management of this eating behaviour. Roy's adaptation model guided this study, which had three objectives:1) to characterize the taste sensitivity (detection and recognition thresholds) to salt in an asymptomatic adult population aged 35 to 60 years, 2) to analyze the relationship between these detection and recognition thresholds and salt consumption, and 3) to explore whether taste sensitivity, salt consumption and the relationship between the two can be altered by gender, age and body composition. To achieve these objectives, detection and recognition thresholds were estimated among participants using a so-called forced choice technique of 1 out of 3 adapted from ASTM. Salt consumption was estimated by 24-hour urinary sodium measurement, as well as three self-reported methods: a discretionary salt intake questionnaire (DSS),a high salt food consumption frequency questionnaire (Na-FFQ), and a 3-day journal. The results obtained show that the salt consumption of Quebec adults in the study is almost twice the maximum tolerable values and at least three times higher than the adequate intake (values recommended by Health Canada), indicating, according to Roy's model, an ineffective behavioural response in this population. Detection and recognition thresholds are associated with salt consumption, but only for measures indicating longer-term nutrient consumption, such as FFQ and discretionary salt, by suggesting an existing relationship between inputs and outputs according to Roy's adaptation model. Salt consumption by most measures used, as well as the detection threshold, is higher for men than for women. Finally, salt consumption and taste thresholds vary according to age and body composition. In conclusion, this research project suggests a relationship between certain sensory properties of salt and its consumption, as well as the influence of factors such as gender and age on this relationship. However, future studies will be necessary to better weight the impact of the taste thresholds assessed in this study with other sensory and hedonic properties, as well as with the multiple other factors that may influence this consumption.
Syarifuddin, Adiansyah. "Multi sensory integration as a strategy to compensate for sodium and fat reduction in food." Thesis, Dijon, 2015. http://www.theses.fr/2015DIJOS088.
Повний текст джерелаIn recent years, health authorities worldwide advise for a reduction of salt and fat in daily food consumption. However, foods with reduced salt and fat content are often not appreciated by consumers, Therefore, the formulation of low-salt-fat foods that maintain acceptability is a major concern in food research. In this thesis, the multi-sensory integration and release kinetics of flavor compounds were explored as strategies to compensate for salt and fat reduction in cheese products (model cheeses and real cheeses). The objective was to better understand the mechanisms leading to aroma and salt release during mastication and to evaluate how the matrix composition and structure influence salt and aroma release profile.Multisensory integration approach to compensate for salt and fat reduction was studied in a first step. The structure and sensory perception of 24 cheese models varying in composition (2 levels of fat, 2 salt, 2 pH at renneting) and flavored with either a sardine aroma (associated to salt), a butter aroma (associated to fat) or not flavoured (control) were characterised by rheological measurements (uniaxial compression) and sensory evaluation (descriptive analysis). The results demonstrated an influence of the composition on the products structure and perceived texture. Furthermore, a significant saltiness enhancement was induced by sardine aroma while significant fat perception enhancement was induced by butter aroma. However, this influence of the aroma on other sensory dimensions depends on the texture of the products thus on their composition and structure. These results have been extended to real cheeses but with specificities. If the sardine flavor enhanced the perception of salt, only butter-sardine-flavor enhanced the perception of fat.In a second step, a physico-chemical approach was developed to explore the release kinetic of flavor compounds during in vitro breakdown using a chewing simulator. The aim was to use these data to explain the influence of the structure of model cheeses and real cheeses and their breakdown during chewing on sensory effects observed in the first step. Volatile compounds release was monitored by connecting the chewing simulator to a proton transfer reaction-mass spectrometry (PTR-MS), while salt release was monitored using a conductivity probe. Results showed that product composition and structure (fat, salt and pH at renneting) influenced aroma release, which however depends on the nature of the aroma: the more hydrophobic compounds are less sensitive to variations in fat content and more sensitive to variations in pH and therefore to the products structure. The salt release kinetic during in vitro chewing was also influenced by the composition and structure of the products. Indeed, beyond salt content which determined the amount of salt released, fat content and the pH at renneting modulated the release kinetic. In conclusion, this work showed a significant impact of the flavor compounds release kinetic and probably of temporality of sensations on the overall perception of salt and fat when consuming a complex food
Zagdoun, Marine. "Caractérisation de l'influence de facteurs environnementaux sur l'écologie microbienne du jambon cuit tranché." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASA003.
Повний текст джерелаReducing salt and nitrite used to limit cooked ham spoilage is a public health concern, but it can lead to changes in microbial ecosystems and enhance ham spoilage. Our objective was to characterize (i) the microbial communities of hams from a production plant, (ii) the production of spoilage metabolites and to predict how those factors would change in the context of a reduction in the salt and the nitrite content. Among the process parameters studied, the slicing line was an important factor. Two profiles of communities were observed at the use-by date according to the slicing line. They were composed of nine species representing 95% of abundance. The presence of those species was correlated with different productions of pyruvate-derived fermentation metabolites.Based on the species identified, we designed a synthetic bacterial community that we inoculated on a synthetic ham-like agar medium with different salt and nitrite concentrations. This synthetic ecology approach showed that a reduction under 12 g/kg of salt and 25-30 mg/kg of nitrite correlated with a stronger species disequilibrium, associated with an exponential Leuconostoc growth and a decreased Carnobacterium growth. This disequilibrium was correlated to a 30% increase of ethanol and D-lactic acid production, which suggests a higher spoilage risk for hams at those concentrations. We then tested those concentrations in industrial condition of production. Microbial behavior was very different according to the profiles of community of hams sliced on the different lines. For the first profile, the behavior of the community was similar to the one observed in the synthetic ecosystem: we observed a disequilibrium of species abundance under 12 g/kg of salt and 25-30 mg/kg of nitrite and a decrease in Carnobacterium divergens abundance. For the second profile, dominated by Serratia proteamaculans, community structure was only slightly affected by a reduction of salt and nitrite. Finally, we observed that reducing the salt and the nitrite content increased yeasts growth.This work highlights the importance of process in the structuration of microbial communities in the final product. This factor is to take into account to understand the impact of any other environmental change on microbial behavior
Boisard, Lauriane. "Relations entre mobilité du sodium, libération du sel et des composés d'arôme en bouche et perception de la flaveur : application à des modèles fromagers." Phd thesis, Université de Bourgogne, 2012. http://tel.archives-ouvertes.fr/tel-00877715.
Повний текст джерелаCabanis, Marie-Thérèse. "Contribution a l'étude du dosage des éléments traces dans les aliments par absorption atomique sans flamme : cas particulier du cadmium." Montpellier 1, 1985. http://www.theses.fr/1985MON13507.
Повний текст джерелаLaberge-Carignan, Audrey, and Audrey Laberge-Carignan. "Élaboration d'une stratégie analytique de dissolution du Hg et du Pb dans les aliments sur le terrain." Master's thesis, Université Laval, 2020. http://hdl.handle.net/20.500.11794/38101.
Повний текст джерелаLa nourriture issue de la chasse ou de la pêche comporte plusieurs nutriments essentiels à une bonne santé. Toutefois ces aliments peuvent aussi contenir des concentrations importantes en contaminants tel que le mercure et le plomb, qui posent un risque pour la santé. Pour guider les communautés qui basent leur alimentation sur la chasse ou la pêche, l’objectif global du projet est de développer une plateforme analytique permettant le suivi de la qualité alimentaire dans les environnements nordiques. Pour ce faire, une stratégie analytique de dissolution de la chair animale pouvant être employée à l’extérieur d’un laboratoire par des non scientifiques a été mise en place. Cette stratégie comporte l’échantillonnage, la mise en solution de la chair et la séparation des analytes pour effectuer leur analyse ainsi que le changement de matrice, en plus de la détection et de la quantification des analytes par fluorimétrie. Toutefois, puisque les systèmes de détection visés pour la plateforme sont en développement, une méthode de quantification du plomb et du mercure par ICP-MS/MS a aussi été développée. Cette stratégie analytique a été élaborée pour l’analyse de l’omble chevalier, un poisson consommé à l’année dans certaines communautés nordiques dans lequel les concentrations en contaminants sont très variables. Le processus analytique, de l’échantillonnage à l’obtention d’une solution mono élémentaire en passant par l’analyse ICP-MS/MS et la dissolution de la chair d’omble chevalier, sera décrit dans ce mémoire.
Fish and game meat contain different essential nutriments for a good health. However, it can also contain contaminants, such as mercury and lead, that pose a health risk. To guide the communities that base their diet on hunting or fishing, the main objective of the project is to develop a portable analytical platform that allows the monitoring of contamination by heavy metals of country food on the field. To this end, an analytical strategy for the dissolution of food flesh that can be deployed outside of the laboratory and used by non-scientists has been established. This strategy includes sampling, flesh dissolution, analyte separation and matrix exchange for the analysis in addition to fluorimetric detection and quantitation of the analytes. Since the quantification system for this platform is still under development, we therefore developed an ICP-MS/MS method to quantify lead and mercury in our food extracts. This method was developed to analyze arctic char, a fish consumed all year long in some northern communities and in which the contamination level can vary significantly. The analytical process from sampling to monoelemental analysis by ICP-MS/MS will be discussed in this document.
Fish and game meat contain different essential nutriments for a good health. However, it can also contain contaminants, such as mercury and lead, that pose a health risk. To guide the communities that base their diet on hunting or fishing, the main objective of the project is to develop a portable analytical platform that allows the monitoring of contamination by heavy metals of country food on the field. To this end, an analytical strategy for the dissolution of food flesh that can be deployed outside of the laboratory and used by non-scientists has been established. This strategy includes sampling, flesh dissolution, analyte separation and matrix exchange for the analysis in addition to fluorimetric detection and quantitation of the analytes. Since the quantification system for this platform is still under development, we therefore developed an ICP-MS/MS method to quantify lead and mercury in our food extracts. This method was developed to analyze arctic char, a fish consumed all year long in some northern communities and in which the contamination level can vary significantly. The analytical process from sampling to monoelemental analysis by ICP-MS/MS will be discussed in this document.
Joannis, Claire. "Médicaments et leurs résidus dans les aliments." Paris 5, 1992. http://www.theses.fr/1992PA05P206.
Повний текст джерелаNasreddine, Lara. "Nouvelles données en sécurité sanitaire des aliments au Liban : Définition du panier de la ménagère Beyrouthin : Evaluation de la contamination des aliments en radionucléides : Evaluation de l'exposition aux métaux lourds par voie alimentaire." Brest, 2005. http://www.theses.fr/2005BRES2010.
Повний текст джерелаThe present study is the first in Lebanon to evaluate, by the market basket approach, the contamination of foods by radionuclides and heavy metals and the dietary exposure of the Lebanese population to the heavy metals Pb, Cd and Hg. An individual dietary survey has been realized on 444 adult individuals living in Beirut. Food consumption data were collected using a quantitative food frequency questionnaire. The market basket was identified by selecting those foods with a mean daily intake exceeding 1g/day. The contamination levels of the selected foods with heavy metals and radionuclides were assessed. The results show that the level of radioactivity of the analyzed food does not present any concern for public health. The average dietary exposure of the average individual to Pb, Cd, and Hg corresponds to 7,17 and 6 % of the respective PTWIs. This permits to conclude that, for the average individual, there is no risk of exceeding the PTWIs
Laberge-Carignan, Audrey. "Élaboration d'une stratégie analytique de dissolution du Hg et du Pb dans les aliments sur le terrain." Master's thesis, Université Laval, 2020. http://hdl.handle.net/20.500.11794/38101.
Повний текст джерелаFish and game meat contain different essential nutriments for a good health. However, it can also contain contaminants, such as mercury and lead, that pose a health risk. To guide the communities that base their diet on hunting or fishing, the main objective of the project is to develop a portable analytical platform that allows the monitoring of contamination by heavy metals of country food on the field. To this end, an analytical strategy for the dissolution of food flesh that can be deployed outside of the laboratory and used by non-scientists has been established. This strategy includes sampling, flesh dissolution, analyte separation and matrix exchange for the analysis in addition to fluorimetric detection and quantitation of the analytes. Since the quantification system for this platform is still under development, we therefore developed an ICP-MS/MS method to quantify lead and mercury in our food extracts. This method was developed to analyze arctic char, a fish consumed all year long in some northern communities and in which the contamination level can vary significantly. The analytical process from sampling to monoelemental analysis by ICP-MS/MS will be discussed in this document.
Duffour, Jacqueline. "Résidus des dithiocarbamates et de l'éthylènethiourée : aspects toxicologiques et analytiques." Montpellier 1, 1991. http://www.theses.fr/1991MON13511.
Повний текст джерелаEl-Bidaoui, Maha. "L'effet de la procédure analytique sur les résidus des pesticides dans les aliments." Aix-Marseille 3, 2002. http://www.theses.fr/2002AIX30068.
Повний текст джерелаThe components of typical procedures for pesticide residues analysis to determine their uncertainty are studied. Procedures for the evaluation of the efficiency and the uncertainty of sample processing, extraction, clean up and determination are developed based on radiotracer and chromatographic techniques with representative foodstuffs and analytes. Sample processing uncertainty is evaluated based on the determination of the 14C activities from test portions of different sizes and estimation of the sampling constants. Different procedures are investigated to decrease the uncertainty of sample processing. A significant finding was that the efficiency of processing varied with the type of equipment and foodstuffs. A synergistic effect was observed by double processing with addition of dry ice. A methodology is developed to study the effects of processing and storage on the pesticide residues' stability. The efficiency and uncertainty of the clean up are studied at different temperatures. The quantification procedures showed that the associated uncertainty is a major contributor to the overall uncertainty and is dependent upon the regression model
Paquin, Julie. "Étude de l'effet de la consommation d'un jus enrichi en fibres alimentaires sur la satiété et la réponse glycémique." Thesis, Université Laval, 2008. http://www.theses.ulaval.ca/2008/25243/25243.pdf.
Повний текст джерелаArnaud, Nicole. "Composition minérale des baies de raisins frontonnais : influence des cépages, des porte-greffes, du sol et du climat." Toulouse 3, 1987. http://www.theses.fr/1987TOU32123.
Повний текст джерелаBéguin, Sylvie. "Développement d'une méthode d'optimisation des conditions de détection GC/EI/MS/MS à l'aide d'un piège ionique quadripolaire : application au dosage multi-résidus de pesticides." Angers, 2002. http://www.theses.fr/2001ANGE0016.
Повний текст джерелаLarauche, Muriel. "Régulations des processus inflammatoires gastriques : rôle des nitrates alimentaire et de l'innervation afférente." Toulouse 3, 2003. http://www.theses.fr/2003TOU30100.
Повний текст джерелаSigala, Frédéric. "Etude de l'influence du sodium, apporté par une irrigation estivale, sur l'évolution de la couche superficielle d'un sol argileux sous l'effet de la pluie." Avignon, 1987. http://www.theses.fr/1987AVIG0003.
Повний текст джерелаNouvel, Cécile. "Synthèse contrôlée de copolymères dextrane-g-polylactide : de leur utilisation comme surfactifs biodégradables à la mise en œuvre de systèmes de vectorisation particulaires." Vandoeuvre-les-Nancy, INPL, 2002. http://docnum.univ-lorraine.fr/public/INPL_T_2002_NOUVEL_C.pdf.
Повний текст джерелаSynthesis of novel grafted amphiphilic copolymers polylactide-grafted dextran was achieved. Macromolecular control of such biodegradable and potentially biocompatible copolymers requires a three-step synthesis based on the "grafting from" concept : partial protection of the dextran hydroxyl groups by silylation ; followed by ring opening polymerization of D, L-lactide initiated from remaining hydroxyls of this partially silylated dextran backbone. The third step involves the silylethers deprotection under very mild conditions. Throughout the synthesis, detailed studies of each step have led to stability of dextran backbone and control of copolymer architecture in terms of graft number and graft length. Tension and fluorescence experiments have given information about the organisation properties of these copolymers. The potential of such copolymers for formulation of nanospheres has been also studied
David, Lucas. "Sodium-water reaction : a heterogeneous runaway process." Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0580.
Повний текст джерелаThe reaction of sodium metal with water is a heterogeneous reaction: reactants are in two distinct condensed phases, separated by a layer of gas. In such configuration, a runaway of the reaction is theoretically not possible, yet experimental observations show the opposite. An explosive runaway of sodium-water reaction can indeed be triggered, generating pressure waves and ejecting matter. A mechanism is proposed to explain these observations by sodium vaporization. As a consequence of the heat generated by the heterogeneous reaction, sodium would vaporize intensely and mix with water vapor in gas-phase, which would generate a homogeneous configuration enabling a runaway. The study of this mechanism is presented through a three-way approach: analytical, numerical and experimental. An analytical, Semenov-type 0d model, adapted to the heterogeneous configuration, first demonstrates that this mechanism can result in a reaction runaway. Numerical simulations of sodium-water reaction, in 1d and in 2d with the “diffuse-interface” approach, then show that this mechanism can produce explosive effects such as shock waves and ejection of matter. Finally, an experimental study confirms the presence of sodium vapor, although the temperature measured inside the liquid sodium is far from its boiling temperature. This study therefore shows that the explosive effects of the sodium-water reaction can theoretically be explained by a rapid vaporization of sodium, although as experimental results suggest, the actual phenomenology is probably more complex. It possibly involves subscale phenomena such as turbulence or fragmentation mechanisms that were not considered in this study
Barchechath, Jérémie, and Jérémie Barchechath. "Étude du potentiel nutraceutique des fibres de canneberge et de bleuet." Doctoral thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/25348.
Повний текст джерелаLes résidus fibreux de canneberge et de bleuet issus du processus d’extraction des polyphénols ont été étudiés pour tenter de les valoriser pour des applications nutraceutiques. Ces travaux se sont plus précisément intéressés aux fibres alimentaires et à leurs polyphénols associés. Ces deux composantes sont reconnues pour leurs effets positifs sur le système digestif dont notamment le maintien de la santé de la flore intestinale. Les résidus fibreux de canneberge et de bleuet obtenus en laboratoire ont été caractérisés pour leurs contenus en polysaccharides non-amidonnés totaux (54,3% pour la canneberge et 46,5% pour le bleuet), solubles (respectivement 21,9% et 21,5%), insolubles (respectivement 32,4% et 25%) et leurs compositions respectives en monosaccharides. Les mesures rhéologiques des fibres (capacité de rétention d’eau, d’huile, capacité de gonflement et viscosité) ont montré des propriétés physiques avantageuses en comparaison à d’autres types de fibres. Le contenu en proanthocyanidines non-extractibles fut alors mesuré par lecture spectrophotométrique (1,3% pour les fibres de canneberge et 2,4% pour celles de bleuet). Une technique utilisant un agent chaotrope fut spécifiquement développée pour obtenir une fraction significativement réduite en polyphénols (contenant respectivement 0,6% et 0,9% de proanthocyanidines). Les fibres brutes et réduites en polyphénols ont alors été administrées à des souris pour tester leurs impacts respectifs sur la flore intestinale. L’ajout de fibres brutes a fait augmenter la concentration de Clostridium coccoides et de Clostridium leptum et diminuer le compte de Lactobacillus alors que celui de Faecalibacterium praunsnitzii a uniquement augmenté avec les fibres réduites en polyphénols mettant en avant un probable effet inhibiteur des polyphénols sur F. prausnitzii. Des souches probiotiques ont alors été cultivées in vitro avec des fibres natives et réduites en proanthocyanidines. Un effet bifidogène ainsi qu’une augmentation de la croissance de Lactobacillus salivarius ont été observés lors de l’ajout de fibres de canneberge et de bleuet. Selon le type de bactérie, les effets différentiels observés avec les fibres brutes ou réduites en proanthocyanidines montrent la capacité de la flore à métaboliser les polysaccharides et leurs polyphénols associés mais aussi un potentiel effet inhibiteur des composés phénoliques. Les fibres de canneberge et de bleuet pourraient ainsi constituer des prébiotiques favorisant indirectement la réduction du risque lié à certaines maladies chroniques intestinales.
The process of polyphenol extraction from cranberry and blueberry yields a fibrous matter that was studied for a potential valorization in nutraceutical applications. More precisely, this work focuses on the dietary fiber content and its associated polyphenols. These two compounds were acknowledged for their positive effects over the gastro-intestinal tract including the preservation of a healthy colonic microbiota. The polyphenol extraction process was replicated in laboratory in order to obtain cranberry and blueberry fibrous matter. These extracts were analysed for their contents in total non-starch polysaccharides (NSP) (54,3% for cranberry and 46,5% for blueberry), soluble NSP (respectively 21,9% and 21,5%), insoluble NSP (respectively 32,4% and 25%) and their respective monosaccharides composition. Rheological measurements (water and oil retention capacities, swelling capacity and viscosity) showed advantageous physical properties compared to other types of fibers. Subsequently, the non-extractable proanthocyanidins contents were quantified by spectrophotometric reading (1,3% for cranberry fibers and 2,4% for blueberry fibers). A method, employing a chaotropic agent, was specifically designed to produce fibers with a significantly reduced content in non-extractable proanthocyanidins (respectively containing 0,6% and 0,9% of PAC). Both native and modified fibers were fed to mice in order to assess their respective impact on intestinal flora. The addition of native fibers was associated with an increase in the concentration of Clostridium coccoides and Clostridium leptum and a diminution of the count of Lactobacillus whereas the concentration of Faecalibacterium praunsnitzii was exclusively increased by the use of fibers reduced in polyphenols. This observation highlighted a potential inhibitory effect of polyphenols on F. prausnitzii. Probiotics strains were therefore cultured in vitro with native and modified fibers. A bifidogenic effect and an increase of the Lactobacillus salivarius concentration were observed when substrate was cranberry or blueberry fibers. According to the type of bacteria, the differential effect monitored between native and proanthocyanidins reduced fibers underlies the flora capacity to metabolize fibers and their associated polyphenols and also a potential inhibitory effect from phenolic compounds. The cranberry and blueberry fibers could therefore constitute prebiotics that favor indirectly the reduction of gastro-intestinal diseases risks.
The process of polyphenol extraction from cranberry and blueberry yields a fibrous matter that was studied for a potential valorization in nutraceutical applications. More precisely, this work focuses on the dietary fiber content and its associated polyphenols. These two compounds were acknowledged for their positive effects over the gastro-intestinal tract including the preservation of a healthy colonic microbiota. The polyphenol extraction process was replicated in laboratory in order to obtain cranberry and blueberry fibrous matter. These extracts were analysed for their contents in total non-starch polysaccharides (NSP) (54,3% for cranberry and 46,5% for blueberry), soluble NSP (respectively 21,9% and 21,5%), insoluble NSP (respectively 32,4% and 25%) and their respective monosaccharides composition. Rheological measurements (water and oil retention capacities, swelling capacity and viscosity) showed advantageous physical properties compared to other types of fibers. Subsequently, the non-extractable proanthocyanidins contents were quantified by spectrophotometric reading (1,3% for cranberry fibers and 2,4% for blueberry fibers). A method, employing a chaotropic agent, was specifically designed to produce fibers with a significantly reduced content in non-extractable proanthocyanidins (respectively containing 0,6% and 0,9% of PAC). Both native and modified fibers were fed to mice in order to assess their respective impact on intestinal flora. The addition of native fibers was associated with an increase in the concentration of Clostridium coccoides and Clostridium leptum and a diminution of the count of Lactobacillus whereas the concentration of Faecalibacterium praunsnitzii was exclusively increased by the use of fibers reduced in polyphenols. This observation highlighted a potential inhibitory effect of polyphenols on F. prausnitzii. Probiotics strains were therefore cultured in vitro with native and modified fibers. A bifidogenic effect and an increase of the Lactobacillus salivarius concentration were observed when substrate was cranberry or blueberry fibers. According to the type of bacteria, the differential effect monitored between native and proanthocyanidins reduced fibers underlies the flora capacity to metabolize fibers and their associated polyphenols and also a potential inhibitory effect from phenolic compounds. The cranberry and blueberry fibers could therefore constitute prebiotics that favor indirectly the reduction of gastro-intestinal diseases risks.
Abed, Aïcha. "Nouveaux dispositifs médicaux à base d'hydrogels de polysaccharides." Paris 13, 2012. http://www.theses.fr/2012PA132062.
Повний текст джерелаThis work describes the development of new medical devices based on hydrogel of polysaccharides (pullulan and dextran). In recent years, our laboratory was interested in the biological properties of synthetic or natural polysaccharides and their interactions with endogenous growth factors and matrix components. So we had the idea to apply their properties to the design of a hybrid material composed of a polypropylene mesh coated with polysaccharides hydrogel. The first objective of this work is to improve the integration of materials currently used in clinical by using of hybrid prosthesis and understand the properties of these hydrogels in vitro and in vivo. In the second part of this work, based on the hydrogel properties a new system of storage and transport of tissue at room temperature was developed while limiting mechanical shocks and contamination associated with leaking fluids. Taking as a model of rat arteries, we validated this system in vitro and in vivo. Finally, we developed a new system for cryopreservation of cells and tissues. This hydrogel based polysaccharides reduces the quantities of toxic cryoprotectants agents during freezing. This method was validated in vitro and in vivo and in comparison with the reference procedure
Raouche, Sana. "Dynamique d’organisation des micelles de caséine et de structuration des gels laitiers : vectorisation de minéraux." Montpellier 2, 2007. http://www.theses.fr/2007MON20226.
Повний текст джерелаIron deficiency is the most frequent nutritional disorder with serious consequences on physical and mental health at individual and nation levels. The aim of the PhD was to solve the technological problems involved in the iron fortification of the dairy products. The work firstly lay in the optimization of the carbonation process and thus in the use of milk as iron vehicle. The reversible acidification process by CO2 injection was studied and optimized. This process, without modifying milk ionic strength, brings elements of comprehension with regard to the dynamic of caseins micelles organization and dairy gel structuring. Micellar calcium phosphate (MCP) reorganization is governed by the carbonation pH, while caseins organization depends on the temperature of treatment. On opposite to enzymatic gelation, “acid” curd formation depends neither on the MCP salt form, nor on caseins organization within micelles in the range of carbonation parameters studied. Reconstituted skimmed milks fortified with FeCl2 and FeCl3 up to 20mmol Fe/kg and subjected or not to carbonation were studied within one day of storage at 30°C. 57Fe Mössbauer spectrometry shows that iron is involved in octahedral coordination with Pi and Po. The carbonation (i) improves the binding yield of iron to casein micelles (ii) the milk rennet clotting time, and (iii) accelerates Fe2+ oxidation. Bioavailability evaluation results are promising but have to be handled with caution
Charon, Hélène. "Détection et quantification d'ajouts de matière grasses végétales autres que le beurre de cacao dans le chocolat." Université Louis Pasteur (Strasbourg) (1971-2008), 2005. http://www.theses.fr/2005STR13119.
Повний текст джерелаThibaudeau, Émilie. "Impact de la réduction du sodium dans une production de fromage Mozzarella." Thesis, Université Laval, 2011. http://www.theses.ulaval.ca/2011/28795/28795.pdf.
Повний текст джерелаThe goal of this project was to quantify the impact of sodium reduction and substitution by KCl and MgCl2 on the production of mozzarella cheese. The reduction of NaCl and its replacement by KCl or MgCl2 did not have an impact on the evolution of the growth and the enzymatic activities of thermophiles lactic bacteria in cheese slurry and mozzarella cheese. Mozzarella cheeses were salted in 12 brines solutions. The concentration of salts in the brine solutions had more impact on the cheese than the type of salts used in the brine. Low sodium mozzarella cheese may be produced without decreasing the quality and functional properties of the cheese, but the substitution of NaCl by KCl may induce a flavour defect in fresh mozzarella cheese, but this cheese could be used as food ingredient without modifying the quality of the final product.
Lestienne, Isabelle. "Contribution à l'étude de la biodisponibilité du fer et du zinc dans le grain de mil et conditions d'amélioration dans les aliments de complément." Montpellier 2, 2004. http://www.theses.fr/2004MON20166.
Повний текст джерелаAmine, Chloe. "Millifluidique à gouttes : un outil pour le criblage des interactions entre biopolymères." Thesis, Nantes, 2017. http://www.theses.fr/2017NANT4120.
Повний текст джерелаLiquid-liquid phase separation of aqueous biopolymers mixtures has been shown to strongly depend on various physico-chemical parameters. One sub-type of liquidliquid phase separation is known as complex coacervation and is primarily driven by attractive electrostatic interactions among two oppositely charged biopolymers. Understanding and characterizing specific conditions and factors leading to phase separated systems is an essential preliminary step in the further development of high values products that take advantage of such phenomenon. However, these screening studies, usually performed in bulk, are highly time consuming and require large quantities of raw materials not always available and sometimes expensive. The aim of this work was to develop a low material consuming droplets-based millifluidic device for the rapid screening of biopolymers interactions. The efficiency of the millifluidic device was demonstrated using a well-known case study of complex coacervation made of a protein-polysaccharide mixture, the ßlactoglobulin/ Gum Arabic mixture. This millifluidic device was then integrated in a multi-technics approach to study a second protein-polysaccharide mixture made of Napin and Pectin. The proposed millifluidic device is easy to implement and is therefore accessible to many laboratories. lt provides a low material consuming approach for rapid screening of biopolymers interactions, which is a prerequisite in the study of phase separation mechanisms
Harkouss, Rami. "Effet du salage et du séchage sur la dynamique d’évolution de la protéolyse, de la structure et de la texture lors de la fabrication d’un jambon sec. Développement d’un modèle de « jambon numérique » couplant transferts d’eau, de sel et protéolyse." Thesis, Clermont-Ferrand 2, 2014. http://www.theses.fr/2014CLF22446/document.
Повний текст джерелаBecause of public health problems, the food industry must lower sodium content in all food products, therefore in cured meat products. During the dry-cured ham elaboration process, decreasing salt content may induce microbial safety problems and texture defects due to an excessive proteolysis that could affect later the industrial stage of slicing. On account of that, this work of thesis aims at (1) studying the relationship between proteolysis, structure and texture during the various stages of dry-cured ham manufacture, and (2) building a “numerical ham” model to predict spatially the time course of water and salt content, and thus water activity (a w ), and to couple these variations with proteolysis. This work combines experimental studies and numerical modelling and simulation. Firstly, a new and powerful technique for quantifying proteolysis that uses “Fluorescamine” was developed and validated on pork meat samples and samples extracted from industrial dry-cured hams; a new proteolysis index (PI) was defined. Based on an experimental design, the time course of proteolysis was quantified in laboratory-salted and dried pork meat samples prepared from five different types of pork muscle. Applying multiple linear regression enabled us to build phenomenological models relating, for each pork muscle, PI velocity to temperature, and to water and salt content. Using Comsol ® Multiphysics software, these phenomenological models were then combined with heat and mass transfer models and associated with calculation of a w , thus constituting the “numerical ham” model. In addition, the time course of PI, five textural parameters (hardness, fragility, cohesiveness, springiness and adhesiveness), and four structural parameters (fiber number, extracellular spaces, cross section area, and connective tissue area) was quantified on samples extracted from two different muscles of industrial dry-cured hams removed from the process at five different processing times. Multiple polynomial regression was applied to build phenomenological models relating PI, salt and water content to some textural and structural parameters investigated. These last models could be rapidly incorporated in the “numerical ham” model to constitute a real process simulator. In the future, the “numerical ham” model should be improved in order to take into account (1) the strong decrease in ham volume due to drying and also (2) the decrease in proteolysis velocity with time as a result of the reduction in the amount of protein that can be hydrolysed in the ham. Once completed and improved, the process simulator will be available to professionals to test scenarios allowing sodium content to be reduced in dry-cured hams without altering their final quality
Seyer, Marie-Ève. "Les fibres alimentaires et le pain de blé entier." Thesis, Université Laval, 2005. http://www.theses.ulaval.ca/2005/23027/23027.pdf.
Повний текст джерелаAlary, Rémi. "Isolement, caractérisation et importance technologique de deux gluténines de faible poids moléculaire chez le blé dur." Montpellier 2, 1988. http://www.theses.fr/1988MON20010.
Повний текст джерелаAdel, Kathryn. "Indice et charge glycémiques de l'alimentation et relations avec l'obésité et le syndrome métabolique au sein de l'étude des familles de Québec (QFS)." Master's thesis, Université Laval, 2014. http://hdl.handle.net/20.500.11794/25199.
Повний текст джерелаMany high-carbohydrate foods common to Western diets have been shown to produce a high glycemic response, which may favor weight gain and metabolic syndrome (MetS), but associations between glycemic index (GI), glycemic load (GL) and obesity and MetS remain inconsistent. These associations were examined in the Quebec Family Study. GI and GL were positively associated with obesity, and an increase in GL over time was associated with gains in adiposity in women only. However, some associations were no longer significant when subjects with implausible reported energy intake (rEI) were excluded. GI and GL were generally not associated with MetS or its risk factors. Results suggest that GI and GL are associated with obesity in women, and that an increase in the GL of their diet is associated with gains in adiposity over time. They also reinforce the importance of considering implausible rEI in dietary surveys in relation to metabolic disease.
Guillard, Valérie. "Characterization and modelling of moisture transfer in composite foods constituted of fresh filling nd cereal-based food : barrier performance of edible films placed at the interface." Montpellier 2, 2003. http://www.theses.fr/2003MON20006.
Повний текст джерелаLacombe, Stéphanie. "Variabilité de la teneur et de la composition en isoflavones de la graine de soja (Glycine max. L. Merrill) et de ses dérivés alimentaires." Toulouse, INPT, 2000. http://www.theses.fr/2000INPT023A.
Повний текст джерелаBouvier, Nathalie. "Le soja : impact en nutrition et médecine humaine." Paris 5, 1998. http://www.theses.fr/1998PA05P145.
Повний текст джерелаVanin, Fernanda M. "Formation de la croûte du pain en cours de cuisson, propriétés rhéologiques et séchage en surface : une approche expérimentale de modélisation." AgroParisTech, 2009. http://www.theses.fr/2009AGPT0062.
Повний текст джерелаTrichaiyaporn, Sommai. "Contribution à l'étude du séchage en discontinu de sucre cristallisé en lit fluidisé." Toulouse, INPT, 1991. http://www.theses.fr/1991INPT043G.
Повний текст джерелаGraillot, Vanessa. "Appréciation quantitative de l'exposition alimentaire à des mélanges de pesticides et mécanismes de génotoxicité." Toulouse 3, 2012. http://thesesups.ups-tlse.fr/2077/.
Повний текст джерелаConsumers are exposed to several pesticide residues present simultaneously in food. This multi-exposure is insufficiently characterized and risk assessment procedures do not take into account appropriately the possible effects of contaminant mixtures. The purpose of this study was to investigate in vitro the genotoxic potential of pesticide mixtures relevant to the dietary exposure of the French population. In order to determine these mixtures, the French population co-exposure was estimated considering both food residue patterns originating from French food monitoring programs and French consumption survey (INCA2). Then, a statistic model was developed by ANSES to compare co-exposures, in order to define groups of individuals with similar pesticide exposure patterns. Based on these results, 7 relevant mixtures were determined and their genotoxic potential was screened in 4 different human cell lines (ACHN, HepG2, LS-174T, SHSY-5Y), using the genotoxic H2AX assay. Mixtures were tested at both equimolar concentrations and proportions reflecting the real diet contamination. The results were compared with the effects of individual compounds. One mixture, consisting of 5 compounds, was found to be genotoxic, only for HepG2. Data obtained with individual compounds showed that only two compounds from the mixture, namely fludioxonil and cyprodinil, were genotoxic. A mixture effect was observed at low doses. The screening of additional pesticides selected from the co-exposure characterization allowed to identify a family of pesticides (methyl-pyrazoles) able to induce DNA double strand break on two human cell lines (Jurkat/SH-SY5Y)
Reynaud, Patricia. "Analyses des résidus d'une triazole dans l'alimentation." Aix-Marseille 1, 1991. http://www.theses.fr/1991AIX11391.
Повний текст джерелаPakin, Caroline. "Le dosage de vitamines du groupe B (acide pantothénique et cobalamines) dans les aliments après isolement chromatographique et détection fluorimétrique." Université Louis Pasteur (Strasbourg) (1971-2008), 2004. https://publication-theses.unistra.fr/public/theses_doctorat/2004/PAKIN_Caroline_2004.pdf.
Повний текст джерелаThe developped method for the free pantothenic acid consists of a purification of the samples by successive passages through anion and cation exchange cartridges, then an isolation by reversed-phase chromatography followed by a post-column derivatization of pantothenic acid as a fluorescent isoindole (reaction of -alanin, formed by hot alkaline hydrolysis of pantothenic acid, with ortho-phthaldialdehyde in the presence of mercaptopropionic acid). An extraction procedure in two steps, first treatment by pepsin (hydrolysis of forms bound to proteins), then treatment by alkaline phosphatase and pantetheinase (hydrolysis of coenzyme A), allows to release the total vitamin B5 in the pantothenic acid form. As for free vitamin B12, the proposed protocol contains a very specific purification by passage through an immunoaffinity column, with concentration of the extract, then a transformation of the vitamin into ribazole (obtained by hot alkaline hydrolysis followed by a treatment by alkaline phosphatase). The fluorescent compound is then analysed by fluorimetry after a separation by reversed-phase chromatography. An enzymatic hydrolysis by pepsin allows to release the different forms of vitamin B12 bound to proteins and thus to obtain an estimation of the total vitamin B12 amount. These sensitive and repetable methods give good recovery rates. Owing to their low detection limit (0,65 µg. G-1 for the vitamin B5 and 1 ng. G-1 for the vitamine B12) and the good resolution of the peaks (respectively of pantothenic acid and of ribazole), they could most probably be applied to the determination of these vitamins in any foodstuff
Barrek, Sami. "Développements de méthodes analytiques pour l'identification et la caractérisation de nouveaux pesticides d'origine végétale : mécanismes de dégradation : applications à des produits de l'agroalimentaire." Lyon 1, 2002. http://www.theses.fr/2002LYO10098.
Повний текст джерелаMerhi, Maysaloun. "Etude de l'impact de l'exposition à des mélanges de pesticides à faibles doses : caractérisation des effets sur des lignées cellulaires humaines et sur le système hématopoïetique murin." Phd thesis, Toulouse, INPT, 2008. http://oatao.univ-toulouse.fr/7769/1/merhi.pdf.
Повний текст джерелаLe, Floc'h Nathalie. "Régulation nutritionnelle de l'oxydation de la thréonine chez le porc." Aix-Marseille 3, 1995. http://www.theses.fr/1996AIX30028.
Повний текст джерелаDheilly, Emmanuelle. "Dynamique pariétale au cours de l’élaboration de la texture de la pomme : étude pluridisciplinaire et intégrée." Thesis, Nantes, 2016. http://www.theses.fr/2016NANT4028/document.
Повний текст джерелаApple texture is a key quality trait for consumers and food processors. It depends on mechanical properties of tissues that result from tissue architecture, cell turgor pressure and cell walls structure . Apple texture evolves due to structural reorganizations of cell walls by proteins and enzymes having various types of activities regulated in time and in space. It also results from changes in cell water and osmolytes fluxes that affect flow causing turgor pressure. Through a multidisciplinary integrative approach, this study aimed at identifying genes associated with cell walls metabolism and turgor pressure regulation in different populations of apple developing contrasted textures and particularly mealiness. The results revealed significant structural modifications of hemicelluloses and associated genes during apple formation and mealiness development. Two distinct groups of ß-galactosidases were identified and associated with development and having or to fruit ripening suggesting different and having hydrolase and/or transglycosylase activities for these enzymes. Many transporters identified in different fruit development phases emphasize highlight the relationship between turgor pressure and apple texture elaboration . The outcome of this work allow proposing a model of mealiness development in apple and identify candidate genes for future functional studies in the development of apple texture as well as genetic markers for varietal improvement
Milanovic, Mirjana. "Contribution à l’étude de la réaction sodium-eau : application à la télédétection de l’hydrogène." Thesis, Paris 10, 2020. http://www.theses.fr/2020PA100078.
Повний текст джерелаThe common thread of this work has been the safety problems of energy installations, whether they are nuclear, such as the fast neutron channel that uses sodium as a heat transfer fluid, or for hydrogen as a storage medium for renewable energies. An experimental and modelling study undertaken with CEA Cadarache first focused on understanding the poorly understood mechanism of the sodium-water reaction based on the determination of temperature and concentration fields in the immersion of a sodium ball in a volume of water. We have shown that the reaction kinetics characterized by a fairly long latency time, which we have modeled, progresses like the vapour pressure of solid and then liquid sodium until it reaches 250°C, where disintegration occurs and then the explosion, which may be of the Coulomb type. The temperature of the effluent then peaks at 1400°C. In a second part we used the developments we had previously made in the remote sensing of hydrogen by the Raman effect. The remote detection of this odorless and colorless gas is one of the conditions for the development of hydrogen, the "fuel of the future". With the picosecond laser used, and although the Raman effect is particularly weak, we can determine the concentration of a leak and also what is original the temperature of a flame at distances up to a hundred meters
Al-Sayeda, Haifaa. "Transfert d'un insecticide systémique, l'imidaclopride, chez la tomate : implication du transport phloémien." Phd thesis, Toulouse, INPT, 2007. http://oatao.univ-toulouse.fr/7654/1/al_sayeda.pdf.
Повний текст джерелаKuate, Kamga Blaise. "Caractérisation et formulation de nouveaux ingrédients alimentaires à base de complexes de protéines végétales et de polysaccharides." Master's thesis, Université Laval, 2019. http://hdl.handle.net/20.500.11794/37917.
Повний текст джерелаThe objective of this work was to characterize the physicochemical properties of complexes formed of plant proteins and dietary fibers. Nine complexes were produced from three plant protein isolates (two soy isolates and one pea isolate) and two fibers (corn and beet) with protein-polysaccharide (P-PS) ratios 1-1, 2-1 and 4-1 by gradual addition of hydrochloric acid up to a pH of 4. The size, the charge and water and oil absorption capacities of these complexes were evaluated with the aim of developing new ingredients with desired functionalities. The formation of complexes allowed to create a synergistic effect on the water absorption capacity of these new ingredients, which was found to be higher for the complexes based on soybeans and beet fiber (20 and 26% for Soya1-Bet and Soya2-Bet against 9 and 23% for Soya1-Corn and Soya2-Corn). The increase in water absorption was generally lower than oil absorption by more than 35%. The great synergy between the P-PS complex was correlated with a low protein content and smaller complex sizes. The formation of complexes appears as an interesting way to modulate the functionality of proteins and fibers to improve the level of fortification of foods.
Zagre, Noël Marie. "Projet pilote d'introduction de l'huile de palme non raffinée comme source de vitamine A au Burkina Faso : évaluation de l'impact." Montpellier 2, 2002. http://www.theses.fr/2002MON20066.
Повний текст джерелаGuerlava, Patrick. "Réponses physiologiques aux stress thermiques et à l'addition de nisine de clostridium perfringens de type A." Compiègne, 1997. http://www.theses.fr/1997COMP1056.
Повний текст джерелаClostridium perfringens NCTC 8798 type A is a human pathogen involved in food spoilage. Dense resting cells suspensions have been treated with a heat shock (50°C), with a cold shock (4°C) and with a nisin addition, the only bacteriocin officialy recognized as GRAS. Influence of these treatments on cell homeostasis bas been determined by measurement of internaI concentration of potassium and sodium, cell volume (Vi), internaI pH (pHi) and membrane potential (ΔΨ). K+ concentration is very high (250 mM) in Clostridium perfringens, and could be used as a means of indirect determination of cell damages. In all treatments, large leaks of potassium were observed, but efflux rate and mechanisms involved in these movements were different. Leaks of K+ were principally associated to cell death during the heat shock. The cold shock induced a rapid K+ efflux which could be compared to mechanisms observed for other bacteria during hypoosmotic physiological response. K+ movements during the cold shock did not induce intracellular volume change or cytoplasm acidification, and stationary phase cells viability was not affected by low temperatures. After nisin addition, leaks of K+ from Clostridium perfringens cell suspensions, were very fast (less than 5 minutes) and were followed by leaks of ATP and proteins. These leaks resulted from cell damages and were associated with cell death. A nisin concentration of 200 IU/ml reduced an initial population from 99. 9%, after one hour of contact. If a pH dissipation was observed after nisin addition, no dissipation of the membrane potential was measured with the methods used. Influence of a preliminary stabilization process on the efficacy of nisin bas been investigated. We showed that nisin effect on Clostridium perfringens vegetative cells was not modified by addition of NaCI or NaNO2. At contrast, nisin efficacy was pH and temperature dependent
Lussier, Noémie. "Impact d'un procédé industriel de brassage et de refroidissement sur les propriétés texturales et rhéologiques de yogourts brassés : rôle de la teneur en matières grasses et du temps de fermentation." Master's thesis, Université Laval, 2017. http://hdl.handle.net/20.500.11794/27784.
Повний текст джерелаMore and more research subjects are about stirred yogurt but, only few have worked on combined impacts of yogurt production at a pilot scale. The purpose of this project was to determine the combined effects of milk fat (MF) content, fermentation time (FT), type of cooling during stirring, and storage time on the physico-chemical, textural and rheological properties of stirred yogurt. The yogurts were standardized at 4.2% protein and 16.5% total solids (TS), and the MF content was adjusted to values between 0.0% and 3.9%. A fat-free yogurt with 14% TS was used as a control. The FT was set at 3, 4 and 5 hours. After fermentation, yogurt (25 kg) of each tank was stirred using a pilot system that simulated industrial production conditions (tank stirring, pumping, smoothing, cooling) and cooled using a tubular heat exchanger, causing low shear, or a plate heat exchanger, which caused high shear. Syneresis, firmness, hysteresis and apparent viscosity were measured during 34 days of storage at 4 °C. The results showed that MF content and TS had little impact on the physico-chemical, textural and rheological properties of stirred yogurts. A higher MF content reduced syneresis and increased firmness, hysteresis loop areas, and viscosity in stirred yogurts. High shear of the plate heat exchanger led to a reduction in syneresis and an increase in firmness, but without changing the viscosity. Storage led to an increase of firmness and viscosity but, syneresis did not change. The results suggest that the type of cooling system used has an important impact on rheological properties but that the presence of MF at 2.6% and higher makes the yogurt more resistant to the disruption of the protein network caused by high shear during stirring, leading to lower syneresis and higher firmness and viscosity.