Статті в журналах: "Alginate-encapsulated structures"

1

Gao, Yingjun, and Xiangyu Jin. "Dual Crosslinked Methacrylated Alginate Hydrogel Micron Fibers and Tissue Constructs for Cell Biology." Marine Drugs 17, no. 10 (September 28, 2019): 557. http://dx.doi.org/10.3390/md17100557.

Повний текст джерела

Анотація:

As an important natural polysaccharide biomaterial from marine organisms, alginate and its derivatives have shown great potential in the fabrication of biomedical materials such as tissue engineering, cell biology, drug delivery, and pharmaceuticals due to their excellent biological activity and controllable physicochemical properties. Ionic crosslinking is the most common method used in the preparation of alginate-based biomaterials, but ionic crosslinked alginate hydrogels are prone to decompose in physiological solution, which hinders their applications in biomedical fields. In this study, dual crosslinked alginate hydrogel microfibers were prepared for the first time. The ionic crosslinked methacrylated alginate (Alg-MA) hydrogel microfibers fabricated by Microfluidic Fabrication (MFF) system were exposed to ultraviolet (UV) light and covalent crosslink between methacrylate groups avoided the fracture of dual crosslinked macromolecular chains in organizational environment. The chemical structures, swelling ratio, mechanical performance, and stability were investigated. Cell-encapsulated dual crosslinked Alg-MA hydrogel microfibers were fabricated to explore the application in tissue engineering for the first time. The hydrogel microfibers provided an excellent 3D distribution and growth conditions for cells. Cell-encapsulated Alg-MA microfibers scaffolds with functional 3D tissue structures were developed which possessed great potential in the production of next-generation scaffolds for tissue engineering and regenerative medicine.

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Nair, Kalyani, Karen Yan, and Wei Sun. "A multilevel numerical model quantifying cell deformation in encapsulated alginate structures." Journal of Mechanics of Materials and Structures 2, no. 6 (August 1, 2007): 1121–39. http://dx.doi.org/10.2140/jomms.2007.2.1121.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Namgung, Bumseok, Kalpana Ravi, Pooja Prathyushaa Vikraman, Shiladitya Sengupta, and Hae Lin Jang. "Engineered cell-laden alginate microparticles for 3D culture." Biochemical Society Transactions 49, no. 2 (April 16, 2021): 761–73. http://dx.doi.org/10.1042/bst20200673.

Повний текст джерела

Анотація:

Advanced microfabrication technologies and biocompatible hydrogel materials facilitate the modeling of 3D tissue microenvironment. Encapsulation of cells in hydrogel microparticles offers an excellent high-throughput platform for investigating multicellular interaction with their surrounding microenvironment. Compartmentalized microparticles support formation of various unique cellular structures. Alginate has emerged as one of the most dominant hydrogel materials for cell encapsulation owing to its cytocompatibility, ease of gelation, and biocompatibility. Alginate hydrogel provides a permeable physical boundary to the encapsulated cells and develops an easily manageable 3D cellular structure. The interior structure of alginate hydrogel can further regulate the spatiotemporal distribution of the embedded cells. This review provides a specific overview of the representative engineering approaches to generate various structures of cell-laden alginate microparticles in a uniform and reproducible manner. Capillary nozzle systems, microfluidic droplet systems, and non-chip based high-throughput microfluidic systems are highlighted for developing well-regulated cellular structure in alginate microparticles to realize potential drug screening platform and cell-based therapy. We conclude with the discussion of current limitations and future directions for realizing the translation of this technology to the clinic.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Sun, Xubing, Jiayong Zhang, Guowen Ding, and Yaohui You. "Tannin-based biosorbent encapsulated into calcium alginate beads for Cr(VI) removal." Water Science and Technology 81, no. 5 (March 1, 2020): 936–48. http://dx.doi.org/10.2166/wst.2020.178.

Повний текст джерела

Анотація:

Abstract A composite biosorbent (AC-TFR) prepared by encapsulating tannin-formaldehyde resin (TFR) into calcium alginate (AC) beads was used to remove Cr(VI) from an aqueous solution. Various influencing factors, such as TFR dosage, pH, initial Cr(VI) concentration, contact time, temperature and presence of co-ions in the medium, were investigated. The structures and adsorption performances of the adsorbents were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). Compared with other AC-TFR adsorbents, AC-TFR-2 (mass ratio of AC:TFR = 1:1) showed an excellent adsorption capacity based on the efficiency of Cr(VI) removal. The kinetic data fitted to pseudo-second-order and intra-particle diffusion models suggested that the adsorption process was subject to a rate-controlling step. The equilibrium adsorption data fitted well to the Langmuir isotherm model, and the maximum adsorption capacities of AC-TFR-2 were 145.99, 167.22 and 174.52 mg/g at 288, 298, and 308 K, respectively. The thermodynamic parameters revealed that Cr(VI) removal by AC-TFR-2 was endothermic and spontaneous, and the process was chemical adsorption. The mechanism of Cr(VI) removal consisted first of reduction to Cr(III), which has a low toxicity, and then chelation onto AC-TFR-2 via ion exchange.

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Conte, Amalia, Lucia Lecce, Mariapia Iannetti, and Matteo Alessandro Del Nobile. "Study on the Influence of Bio-Based Packaging System on Sodium Benzoate Release Kinetics." Foods 9, no. 8 (July 27, 2020): 1010. http://dx.doi.org/10.3390/foods9081010.

Повний текст джерела

Анотація:

The influence of film structure on the release kinetics of sodium benzoate (SB) from polymeric films is addressed in this study. In particular, four film structures were investigated, two monolayer and two multilayer systems. In particular, in one case, the active substance was uniformly distributed into a chitosan-based matrix, and in the other one, it was previously incorporated into alginate beads before dispersion in the chitosan film, thus realizing two types of monolayer films; on the other hand, the same chitosan film with SB encapsulated in alginate beads was used as the inner layer of a multilayer system constituted by two side films of alginate. The two alginate-based layers were made with two different thicknesses, thus producing a total of two multilayer systems. The release of SB from the above-mentioned films in water was studied by means of a UV/VIS spectrophotometer at 227 nm. A first-order kinetics-type equation was used to quantitatively describe the release data. Results suggest that the film structure strongly affected the release kinetics. In fact, monolayer films showed single-stage release kinetics, whereas the two investigated multilayer systems showed two-stage release kinetics. Further, the presence of alginate beads strongly affected the SB release, thus suggesting the potential of encapsulation to control the release mechanism of active compounds.

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Hamilton, Charles, Gursel Alici, Geoff Spinks, and Marc in het Panhuis. "The Suitability of 3-D Printed Eutectic Gallium-Indium Alloy as a Heating Element for Thermally Active Hydrogels." MRS Advances 2, no. 6 (December 15, 2016): 335–40. http://dx.doi.org/10.1557/adv.2016.618.

Повний текст джерела

Анотація:

ABSTRACTWe report the use of a novel extrusion tip that allows for the omnidirectional printing of eutectic gallium-indium (eGaIn) alloy onto the surface of hydrogel materials into complex 2-dimensional patterns. The use of these printed soft “wires” as an electrothermal heating element for soft robotics purposes was explored. Heating of the eGaIn structures encapsulated in an alginate/acrylamide ionic-covalent entanglement hydrogel was measured by a thermal imaging camera. It was determined that eGaIn is a suitable material for use in future soft robotics applications as an electrothermal heating element to actuate thermally responsive N-isoproylacrylamide hydrogels.

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Cigu, Toni Andor, Mihaela Nicoleta Holban, Anca Niculina Cadinoiu, Valeriu Sunel, Catalina Lionte, Marcel Popa, Jacques Desbrieres, and Corina Cheptea. "Polyelectrolyte Complex Based Nanocapsules Carrying Novel 5-Nitroindazole Thiazolidines with Potential Use in Treating Oral Infections." Materiale Plastice 54, no. 1 (March 30, 2017): 160–67. http://dx.doi.org/10.37358/mp.17.1.4808.

Повний текст джерела

Анотація:

The aim of this research was the synthesis of novel 2,3-disubstituted 1,3 thiazolidines, derived from 5-nitroindazole with antimicrobial activity and their encapsulation into polymer nanocapsules. Starting from previously synthesised hydrazones, there have been obtained novel thiazolidines by reaction with thioglycolic acid. The envisaged chemical structures were confirmed by spectral and elemental analysis. Two of the obtained thiazolidines were encapsulated into cationic Eudragit E100 nanocapsules, obtained by nanoprecipitation. In order to enhance drug release characteristics and particle stability, Eudragit E100 nanocapsules were covered with anionic polysaccharide (sodium alginate), thus forming a complex polyelectrolyte based membrane. The obtained nanocapsules presented a slower and more controlled drug release. The synthesized active principles, in free state and encapsulated into polymer nanocapsules, were tested for their acute toxicity and their influence on the development of model bacterial strains (Staphylococcus mutans, Actinobacillus actinomycetemcomitans, Bacillus subtilis, Bacillus cereus, Salmonella enteritidis, Escherichia coli and Staphylococcus aureus).

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Gryshkov, Oleksandr, Vitalii Mutsenko, Dmytro Tarusin, Diaa Khayyat, Ortwin Naujok, Ekaterina Riabchenko, Yuliia Nemirovska, Arseny Danilov, Alexander Y. Petrenko, and Birgit Glasmacher. "Coaxial Alginate Hydrogels: From Self-Assembled 3D Cellular Constructs to Long-Term Storage." International Journal of Molecular Sciences 22, no. 6 (March 18, 2021): 3096. http://dx.doi.org/10.3390/ijms22063096.

Повний текст джерела

Анотація:

Alginate as a versatile naturally occurring biomaterial has found widespread use in the biomedical field due to its unique features such as biocompatibility and biodegradability. The ability of its semipermeable hydrogels to provide a favourable microenvironment for clinically relevant cells made alginate encapsulation a leading technology for immunoisolation, 3D culture, cryopreservation as well as cell and drug delivery. The aim of this work is the evaluation of structural properties and swelling behaviour of the core-shell capsules for the encapsulation of multipotent stromal cells (MSCs), their 3D culture and cryopreservation using slow freezing. The cells were encapsulated in core-shell capsules using coaxial electrospraying, cultured for 35 days and cryopreserved. Cell viability, metabolic activity and cell–cell interactions were analysed. Cryopreservation of MSCs-laden core-shell capsules was performed according to parameters pre-selected on cell-free capsules. The results suggest that core-shell capsules produced from the low viscosity high-G alginate are superior to high-M ones in terms of stability during in vitro culture, as well as to solid beads in terms of promoting formation of viable self-assembled cellular structures and maintenance of MSCs functionality on a long-term basis. The application of 0.3 M sucrose demonstrated a beneficial effect on the integrity of capsules and viability of formed 3D cell assemblies, as compared to 10% dimethyl sulfoxide (DMSO) alone. The proposed workflow from the preparation of core-shell capsules with self-assembled cellular structures to the cryopreservation appears to be a promising strategy for their off-the-shelf availability.

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Wróblewska-Krepsztul, Jolanta, Tomasz Rydzkowski, Iwona Michalska-Pożoga, and Vijay Kumar Thakur. "Biopolymers for Biomedical and Pharmaceutical Applications: Recent Advances and Overview of Alginate Electrospinning." Nanomaterials 9, no. 3 (March 10, 2019): 404. http://dx.doi.org/10.3390/nano9030404.

Повний текст джерела

Анотація:

Innovative solutions using biopolymer-based materials made of several constituents seems to be particularly attractive for packaging in biomedical and pharmaceutical applications. In this direction, some progress has been made in extending use of the electrospinning process towards fiber formation based on biopolymers and organic compounds for the preparation of novel packaging materials. Electrospinning can be used to create nanofiber mats characterized by high purity of the material, which can be used to create active and modern biomedical and pharmaceutical packaging. Intelligent medical and biomedical packaging with the use of polymers is a broadly and rapidly growing field of interest for industries and academia. Among various polymers, alginate has found many applications in the food sector, biomedicine, and packaging. For example, in drug delivery systems, a mesh made of nanofibres produced by the electrospinning method is highly desired. Electrospinning for biomedicine is based on the use of biopolymers and natural substances, along with the combination of drugs (such as naproxen, sulfikoxazol) and essential oils with antibacterial properties (such as tocopherol, eugenol). This is a striking method due to the ability of producing nanoscale materials and structures of exceptional quality, allowing the substances to be encapsulated and the drugs/ biologically active substances placed on polymer nanofibers. So, in this article we briefly summarize the recent advances on electrospinning of biopolymers with particular emphasis on usage of Alginate for biomedical and pharmaceutical applications.

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Ouyang, Liliang, James P. K. Armstrong, Yiyang Lin, Jonathan P. Wojciechowski, Charlotte Lee-Reeves, Daniel Hachim, Kun Zhou, Jason A. Burdick, and Molly M. Stevens. "Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks." Science Advances 6, no. 38 (September 2020): eabc5529. http://dx.doi.org/10.1126/sciadv.abc5529.

Повний текст джерела

Анотація:

A major challenge in three-dimensional (3D) bioprinting is the limited number of bioinks that fulfill the physicochemical requirements of printing while also providing a desirable environment for encapsulated cells. Here, we address this limitation by temporarily stabilizing bioinks with a complementary thermo-reversible gelatin network. This strategy enables the effective printing of biomaterials that would typically not meet printing requirements, with instrument parameters and structural output largely independent of the base biomaterial. This approach is demonstrated across a library of photocrosslinkable bioinks derived from natural and synthetic polymers, including gelatin, hyaluronic acid, chondroitin sulfate, dextran, alginate, chitosan, heparin, and poly(ethylene glycol). A range of complex and heterogeneous structures are printed, including soft hydrogel constructs supporting the 3D culture of astrocytes. This highly generalizable methodology expands the palette of available bioinks, allowing the biofabrication of constructs optimized to meet the biological requirements of cell culture and tissue engineering.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Campiglio, Chiara, Francesca Ceriani, and Lorenza Draghi. "3D Encapsulation Made Easy: A Coaxial-Flow Circuit for the Fabrication of Hydrogel Microfibers Patches." Bioengineering 6, no. 2 (April 6, 2019): 30. http://dx.doi.org/10.3390/bioengineering6020030.

Повний текст джерела

Анотація:

To fully exploit the potential of hydrogel micro-fibers in the design of regenerative medicinal materials, we designed a simple, easy to replicate system for cell embedding in degradable fibrous scaffolds, and validated its effectiveness using alginate-based materials. For scaffold fabrication, cells are suspended in a hydrogel-precursor and injected in a closed-loop circuit, where a pump circulates the ionic cross-linking solution. The flow of the cross-linking solution stretches and solidifies a continuous micro-scaled, cell-loaded hydrogel fiber that whips, bends, and spontaneously assembles in a self-standing, spaghetti-like patch. After investigation and tuning of process- and solution-related parameters, homogeneous microfibers with controlled diameters and consistent scaffolds were obtained from different alginate concentrations and blends with biologically favorable macromolecules (i.e., gelatin or hyaluronic acid). Despite its simplicity, this coaxial-flow encapsulation system allows for the rapid and effortless fabrication of thick, well-defined scaffolds, with viable cells being homogeneously distributed within the fibers. The reduced fiber diameter and the inherent macro-porous structure that is created from the random winding of fibers can sustain mass transport, and support encapsulated cell survival. As different materials and formulations can be processed to easily create homogeneously cell-populated structures, this system appears as a valuable platform, not only for regenerative medicine, but also, more in general, for 3D cell culturing in vitro.

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Del Vento, Federico, Jonathan Poels, Maxime Vermeulen, Bernard Ucakar, Maria Grazia Giudice, Marc Kanbar, Anne des Rieux, and Christine Wyns. "Accelerated and Improved Vascular Maturity after Transplantation of Testicular Tissue in Hydrogels Supplemented with VEGF- and PDGF-Loaded Nanoparticles." International Journal of Molecular Sciences 22, no. 11 (May 28, 2021): 5779. http://dx.doi.org/10.3390/ijms22115779.

Повний текст джерела

Анотація:

Avascular transplantation of frozen–thawed testicular tissue fragments represents a potential future technique for fertility restoration in boys with cancer. A significant loss of spermatogonia was observed in xeno-transplants of human tissue most likely due to the hypoxic period before revascularization. To reduce the effect of hypoxia–reoxygenation injuries, several options have already been explored, like encapsulation in alginate hydrogel and supplementation with nanoparticles delivering a necrosis inhibitor (NECINH) or VEGF. While these approaches improved short-term (5 days) vascular surfaces in grafts, neovessels were not maintained up to 21 days; i.e., the time needed for achieving vessel stabilization. To better support tissue grafts, nanoparticles loaded with VEGF, PDGF and NECINH were developed. Testicular tissue fragments from 4–5-week-old mice were encapsulated in calcium-alginate hydrogels, either non-supplemented (control) or supplemented with drug-loaded nanoparticles (VEGF-nanoparticles; VEGF-nanoparticles + PDGF-nanoparticles; NECINH-nanoparticles; VEGF-nanoparticles + NECINH-nanoparticles; and VEGF-nanoparticles + PDGF-nanoparticles + NECINH-nanoparticles) before auto-transplantation. Grafts were recovered after 5 or 21 days for analyses of tissue integrity (hematoxylin–eosin staining), spermatogonial survival (immuno-histo-chemistry for promyelocytic leukemia zinc finger) and vascularization (immuno-histo-chemistry for α-smooth muscle actin and CD-31). Our results showed that a combination of VEGF and PDGF nanoparticles increased vascular maturity and induced a faster maturation of vascular structures in grafts.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Lee, Su Jeong, Ji Min Seok, Jun Hee Lee, Jaejong Lee, Wan Doo Kim, and Su A. Park. "Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink." Polymers 13, no. 5 (March 5, 2021): 794. http://dx.doi.org/10.3390/polym13050794.

Повний текст джерела

Анотація:

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Yao, Yuanhang, Jiaxing, Jansen Lin, Mei Hui Liu, Saif A. Khan, and Jung Eun Kim. "Evaluation of Stability and In-Vitro Bioaccessibility of Encapsulated Lutein via Microfluidic Technology." Current Developments in Nutrition 5, Supplement_2 (June 2021): 80. http://dx.doi.org/10.1093/cdn/nzab034_014.

Повний текст джерела

Анотація:

Abstract Objectives Inadequate intake of lutein was relevant to a higher risk of aging-related eye disease. Since lutein cannot be synthesized in body, it should be obtained from the food. However, lutein has been barely incorporated into food because it is prone to degradation and is poor bioaccessible in the gastrointestinal tract. Thus, this present study aimed to encapsulate lutein in staple food using excipient emulsions via a novel microfluidic technique and to assess the stability and bioaccessibility of lutein. Methods A combination of alginate and soy protein isolate was applied as food ingredients for fabricating structured encapsulation purposes and two types of oil (safflower oil (SO) and olive oil (OL)) were selected as a delivery vehicle for lutein. Two customized microfluidic devices (co-flow and combination-flow) were assembled to encapsulate lutein into food structures that mimic noodle, an Asian staple food. The extruded microfluidic noodle was created by the following: co-flow + SO, co-flow + OL, combination-flow + SO and combination-flow + OL. The stability of lutein from the microfluidic noodle was evaluated under 4°C storage for one week. The bioaccessibility of lutein was also investigated via a simulated in-vitro gastrointestinal model and lutein was detected by high-performance liquid chromatography. Results The successful encapsulation of lutein in noodle-like structures via microfluidic techniques was achieved at 86.0 ± 5.8% (mean ± SD). Although lutein's stability experienced a decreasing trend, the retention of lutein maintained above 60% up to one week's storage in all types of microfluidic noodle. However, two types of device did not result in a difference in lutein bioaccessibility (co-flow: 3.1 ± 0.5%, combination-flow: 3.6 ± 0.6%, mean ± SD) and SO and OL also showed no differences in lutein bioaccessibility (SO: 3.4 ± 0.8%, OL: 3.3 ± 0.4%, mean ± SD). Conclusions Lutein is successfully encapsulated in new noodle-like food products using excipient emulsions via a novel microfluidic technology and is relatively stable for up to one week's storage. However, findings from this study suggest that the types of oil and device do not affect the lutein bioaccessibility. Funding Sources National University of Singapore, Cross-Faculty Research Grant; Agency for Science, Technology and Research.

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Carreras, Pilar, Itziar González, Miguel Gallardo, Alejandra Ortiz-Ruiz, Maria Luz Morales, Jessica Encinas, and Joaquín Martínez-López. "Long-Term Human Hematopoietic Stem Cell Culture in Microdroplets." Micromachines 12, no. 1 (January 16, 2021): 90. http://dx.doi.org/10.3390/mi12010090.

Повний текст джерела

Анотація:

We previously reported a new approach for micromanipulation and encapsulation of human stem cells using a droplet-based microfluidic device. This approach demonstrated the possibility of encapsulating and culturing difficult-to-preserve primary human hematopoietic stem cells using an engineered double-layered bead composed by an inner layer of alginate and an outer layer of Puramatrix. We also demonstrated the maintenance and expansion of Multiple Myeloma cells in this construction. Here, the presented microfluidic technique is applied to construct a 3D biomimetic model to recapitulate the human hematopoietic stem cell niche using double-layered hydrogel beads cultured in 10% FBS culture medium. In this model, the long-term maintenance of the number of cells and expansion of hHSCS encapsulated in the proposed structures was observed. Additionally, a phenotypic characterization of the human hematopoietic stem cells generated in the presented biomimetic model was performed in order to assess their long-term stemness maintenance. Results indicate that the ex vivo cultured human CD34+ cells from bone marrow were viable, maintained, and expanded over a time span of eight weeks. This novel long-term stem cell culture methodology could represent a novel breakthrough to improve Hematopoietic Progenitor cell Transplant (HPT) as well as a novel tool for further study of the biochemical and biophysical factors influencing stem cell behavior. This technology opens a myriad of new applications as a universal stem cell niche model potentially able to expand other types of cells.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Chowdhury, Shakhawat, Imran Rahman Chowdhury, Fayzul Kabir, Mohammad Abu Jafar Mazumder, Md Hasan Zahir, and Khalid Alhooshani. "Alginate-based biotechnology: a review on the arsenic removal technologies and future possibilities." Journal of Water Supply: Research and Technology-Aqua 68, no. 6 (July 15, 2019): 369–89. http://dx.doi.org/10.2166/aqua.2019.005.

Повний текст джерела

Анотація:

Abstract The alginate-based adsorption technologies have emerged as potential methods for arsenic removal from drinking water. The adsorbents (iron oxide, hydroxide, nano zero valent iron (nZVI), industrial waste, minerals, magnetite, goethite, zirconium oxide, etc.) are impregnated into alginate beads to produce the media. The biocompatibility, rough surface with large area, and amorphous and high water permeable bead structure improve arsenic adsorption efficiency while the regeneration process is simpler than the conventional adsorbents. In recent years, studies have reported laboratory-scale applications of alginate beads, encapsulated and impregnated with adsorbents, for arsenic removal from drinking water. The arsenic removal efficiencies were reported to be over 95% with a wide range of concentrations (10–1,000 parts per billion) and pH (3.0–7.5). However, commercial- and/or mass-scale applications have not been reported yet, due possibly to overall cost, complexity, reusability, and arsenic waste-laden sludge management. In this paper, research achievement on arsenic removal using alginate-based adsorbents has been reviewed. The review was performed in context to alginate bead development, adsorbent encapsulation and impregnation, application, performance, and regeneration. The advantages and limitations of the methods were analyzed and the scopes of future research were identified for mass scale domestic and industrial applications.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Abraham, Reinu E., Peng Su, Munish Puri, Colin L. Raston, and Wei Zhang. "Release of encapsulated bioactives influenced by alginate viscosity under in-vitro gastrointestinal model." International Journal of Biological Macromolecules 170 (February 2021): 540–48. http://dx.doi.org/10.1016/j.ijbiomac.2020.12.143.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Wang, Zhiyue, Patricia Perez-Calleja, Robert Nerenberg, Paige J. Novak, and Satoshi Ishii. "Unraveling encapsulated growth of Nitrosomonas europaea in alginate: An experimental and modeling study." Water Research 208 (January 2022): 117857. http://dx.doi.org/10.1016/j.watres.2021.117857.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Sinha, Priyanka, Ubaidulla Udhumansha, Grace Rathnam, Mani Ganesh, and Hyun Tae Jang. "Capecitabine encapsulated chitosan succinate-sodium alginate macromolecular complex beads for colon cancer targeted delivery: in vitro evaluation." International Journal of Biological Macromolecules 117 (October 2018): 840–50. http://dx.doi.org/10.1016/j.ijbiomac.2018.05.181.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Sokolov, A. Y., and D. I. Shishkina. "Study of the structural and mechanical properties of biopolymers in order to obtain a capsule-type product." Proceedings of the Voronezh State University of Engineering Technologies 83, no. 1 (June 3, 2021): 248–52. http://dx.doi.org/10.20914/2310-1202-2021-1-248-252.

Повний текст джерела

Анотація:

The article presents some theoretical and experimental data on promising technologies, namely, the processes of obtaining artificial food materials such as spheres or "caviar". They are derived from molecular processes: solubilization, spherification, etc. Possible applications are the food industry, the food service industry, biotechnology, and others. There are different features of obtaining artificial products based on alginates. The peculiarities of the alginate structuring are that it is possible to form a gel layer-encapsulation and gel formation over the entire thickness of the product due to the special chemical properties of the fixing salt. Based on the theory of the molecular structure of biopolymers, molecular technologies for the synthesis of artificial food products were developed, using the example of molecular "caviar". As a result of our own experiments, we obtained a satisfactory encapsulated product from a biopolymer crosslinked with Ca2+ salts in terms of organoleptic and physico-chemical properties. The colloidal biopolymer solution for forming "eggs" was characterized using the method of rotational viscometry, which showed the features of the sodium alginate solution as a structured thixotropic material, which is characterized by" difficulty " of shear at low speeds of rotation of the viscometer rotor. Further on the rheogram, such material exhibits a predicted relatively stable flow. As a result, it can be used to produce semi-finished products of a given shape and texture as a food semi-finished product or product. If the technology is refined, it is possible to use colloidal systems based on alginates and other biopolymers in biotechnology, including the cultivation of microorganisms of various taxonomic groups.

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Hu, Xinxin, Chunyan Liu, Haitian Zhang, Md Alomgir Hossen, Dur E. Sameen, Jianwu Dai, Wen Qin, Yaowen Liu, and Suqing Li. "In vitro digestion of sodium alginate/pectin co-encapsulated Lactobacillus bulgaricus and its application in yogurt bilayer beads." International Journal of Biological Macromolecules 193 (December 2021): 1050–58. http://dx.doi.org/10.1016/j.ijbiomac.2021.11.076.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Dalponte Dallabona, Ithiara, Gabriel Goetten de Lima, Beatriz Isabella Cestaro, Ivisson de Souza Tasso, Thainnane Silva Paiva, Emanuele Joana Gbur Laureanti, Luiz Mario de Matos Jorge, et al. "Development of alginate beads with encapsulated jabuticaba peel and propolis extracts to achieve a new natural colorant antioxidant additive." International Journal of Biological Macromolecules 163 (November 2020): 1421–32. http://dx.doi.org/10.1016/j.ijbiomac.2020.07.256.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Panichikkal, Jishma, Gopika Prathap, Remakanthan Appukuttan Nair, and Radhakrishnan Edayileveetil Krishnankutty. "Evaluation of plant probiotic performance of Pseudomonas sp. encapsulated in alginate supplemented with salicylic acid and zinc oxide nanoparticles." International Journal of Biological Macromolecules 166 (January 2021): 138–43. http://dx.doi.org/10.1016/j.ijbiomac.2020.10.110.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Chittoo, Beverly S., and Clint Sutherland. "Adsorption Using Lime-Iron Sludge–Encapsulated Calcium Alginate Beads for Phosphate Recovery with ANN- and RSM-Optimized Encapsulation." Journal of Environmental Engineering 145, no. 5 (May 2019): 04019019. http://dx.doi.org/10.1061/(asce)ee.1943-7870.0001519.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Pour, Mojde Moradi, Roohallah Saberi-Riseh, Reza Mohammadinejad, and Ahmad Hosseini. "Investigating the formulation of alginate- gelatin encapsulated Pseudomonas fluorescens (VUPF5 and T17-4 strains) for controlling Fusarium solani on potato." International Journal of Biological Macromolecules 133 (July 2019): 603–13. http://dx.doi.org/10.1016/j.ijbiomac.2019.04.071.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Saberi-Rise, Roohallah, and Mojde Moradi-Pour. "The effect of Bacillus subtilis Vru1 encapsulated in alginate – bentonite coating enriched with titanium nanoparticles against Rhizoctonia solani on bean." International Journal of Biological Macromolecules 152 (June 2020): 1089–97. http://dx.doi.org/10.1016/j.ijbiomac.2019.10.197.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Ahmed Refat El-Mahdy, Mohamed, Ahmed ELShami, Mohamed Yousry Elshikh, and Seleem Saleh Elsayed Ahmad. "Self-healing mortar using different types, content, and concentrations of bacteria to repair cracks." Frattura ed Integrità Strutturale 16, no. 59 (December 22, 2021): 486–513. http://dx.doi.org/10.3221/igf-esis.59.32.

Повний текст джерела

Анотація:

The creation of cracks, which are the most common cause of structural failure, has a significant impact on the structure's strength and durability. As a result, effective repair and maintenance are vital and unavoidable for treating any of these issues. Self-healing mortar holds promising benefits for reducing the cost of repair as cracks are autonomously repaired without any human intervention. This study investigated the effect of bacteria type, bacteria content, bacteria concentration, and nutrient type on the properties of the self-healing mortar. Three types of bacteria, Bacillus sphaericus, Bacillus Megaterium, and Bacillus subtilis encapsulated in calcium alginate beads, were introduced into the mortar. Two concentrations of bacteria, 2× 108 and 2× 109 Colony Forming Units per milliliter, and different percentages of bacteria of cement weight were selected for the study. In addition, calcium lactate and calcium acetate were used at 0.5% of cement weight as nutrition for bacteria. Tests were performed for compressive strength, bending strength, SEM, EDX, and TGA/DTG. The results show a significant development in the mechanical behaviour of mortar, especially with Bacillus Megaterium using a 2.5% bacterial proportion with a concentration 2× 109 CFU/ml. This can be related to the filling of voids and cracks in microbial mortar by calcite, which was confirmed by SEM and EDX.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Jose Varghese, R., Sundararajan Parani, V. R. Remya, Rodney Maluleke, Sabu Thomas, and Oluwatobi S. Oluwafemi. "Sodium alginate passivated CuInS2/ZnS QDs encapsulated in the mesoporous channels of amine modified SBA 15 with excellent photostability and biocompatibility." International Journal of Biological Macromolecules 161 (October 2020): 1470–76. http://dx.doi.org/10.1016/j.ijbiomac.2020.07.240.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Karimi, Sarah, Zohreh Bagher, Najmeh Najmoddin, Sara Simorgh, and Mohamad Pezeshki-Modaress. "Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application." International Journal of Biological Macromolecules 167 (January 2021): 796–806. http://dx.doi.org/10.1016/j.ijbiomac.2020.11.199.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Qi, Xiaoxi, Senay Simsek, Bingcan Chen, and Jiajia Rao. "Alginate-based double-network hydrogel improves the viability of encapsulated probiotics during simulated sequential gastrointestinal digestion: Effect of biopolymer type and concentrations." International Journal of Biological Macromolecules 165 (December 2020): 1675–85. http://dx.doi.org/10.1016/j.ijbiomac.2020.10.028.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Bagheri-Josheghani, Sareh, and Bita Bakhshi. "Formulation of selenium nanoparticles encapsulated by alginate-chitosan for controlled delivery of Vibrio Cholerae LPS: A novel delivery system candidate for nanovaccine." International Journal of Biological Macromolecules 208 (May 2022): 494–508. http://dx.doi.org/10.1016/j.ijbiomac.2022.03.087.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Alaysuy, Omaymah, Razan M. Snari, Alia Abdulaziz Alfi, Afrah M. Aldawsari, Sraa Abu-Melha, Mohamed E. Khalifa, and Nashwa M. El-Metwaly. "Development of green and sustainable smart biochromic and therapeutic bandage using red cabbage (Brassica oleracea L. Var. capitata) extract encapsulated into alginate nanoparticles." International Journal of Biological Macromolecules 211 (June 2022): 390–99. http://dx.doi.org/10.1016/j.ijbiomac.2022.05.062.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Garmia, Derafa, H. Zaghouane-Boudiaf, and César Viseras Ibbora. "Preparation and characterization of new low cost adsorbent beads based on activated bentonite encapsulated with calcium alginate for removal of 2,4-dichlorophenol from aqueous medium." International Journal of Biological Macromolecules 115 (August 2018): 257–65. http://dx.doi.org/10.1016/j.ijbiomac.2018.04.064.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Aldana, Ana A., Marina Uhart, Gustavo A. Abraham, Diego M. Bustos та Aldo R. Boccaccini. "14-3-3ε protein-loaded 3D hydrogels favor osteogenesis". Journal of Materials Science: Materials in Medicine 31, № 11 (листопад 2020). http://dx.doi.org/10.1007/s10856-020-06434-1.

Повний текст джерела

Анотація:

Abstract3D printing has emerged as vanguard technique of biofabrication to assemble cells, biomaterials and biomolecules in a spatially controlled manner to reproduce native tissues. In this work, gelatin methacrylate (GelMA)/alginate hydrogel scaffolds were obtained by 3D printing and 14-3-3ε protein was encapsulated in the hydrogel to induce osteogenic differentiation of human adipose-derived mesenchymal stem cells (hASC). GelMA/alginate-based grid-like structures were printed and remained stable upon photo-crosslinking. The viscosity of alginate allowed to control the pore size and strand width. A higher viscosity of hydrogel ink enhanced the printing accuracy. Protein-loaded GelMA/alginate-based hydrogel showed a clear induction of the osteogenic differentiation of hASC cells. The results are relevant for future developments of GelMA/alginate for bone tissue engineering given the positive effect of 14-3-3ε protein on both cell adhesion and proliferation.

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Palanyandy, Sharrmila Rengeswari, Saikat Gantait, Sreeramanan Subramaniam, and Uma Rani Sinniah. "Cryopreservation of oil palm (Elaeis guineensis Jacq.) polyembryoids via encapsulation–desiccation." 3 Biotech 10, no. 1 (December 3, 2019). http://dx.doi.org/10.1007/s13205-019-1997-9.

Повний текст джерела

Анотація:

AbstractThe current report assesses the efficiency of encapsulation–desiccation protocol to cryopreserve oil palm (Elaeis guineensis Jacq.) polyembryoids. Specifically identified polyembryoids, comprising of haustorium and torpedo-shaped structures, were encapsulated [comprising 3% (w/v) sodium alginate and 100 mM CaCl2]. Calcium alginate-encapsulated and sucrose-precultured polyembryoids were subjected to different spans of desiccation in a laminar air-flow cabinet, followed by freezing in liquid nitrogen. The effect of sucrose preculture (with gradual exposure to 0.3, 0.5, 0.75 and 1 M for 7 days) and dehydration periods (0–10 h) under sterile air-flow on post-freezing survival and regrowth of encapsulated polyembryoids were studied. Cryopreserved and thawed polyembryoids (initially precultured in sucrose, followed by 9 h air-desiccated to 23.3% moisture content) displayed the highest survival percentage (73.3%) and regeneration (of shoot, root and secondary somatic embryo) on Murashige and Skoog regrowth medium containing sucrose (0.3–1 M) and 0.2 mg/l 2,4-dichlorophenoxy acetic acid. In addition, ultrastructural study using scanning electron microscopy exhibited successful revival of cryopreserved polyembryoids, owing to retention of cellular membrane stability through optimized and protected (encapsulated) desiccation. The present study thus substantiates the potential of this encapsulation–desiccation procedure in cryopreservation of oil palm polyembryoids for long-term conservation programs.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Hermann, Katharina M., Alexander Grünberger, and Anant V. Patel. "Formalin-casein enhances water absorbency of calcium alginate beads and activity of encapsulated Metarhizium brunneum and Saccharomyces cerevisiae." World Journal of Microbiology and Biotechnology 37, no. 9 (August 18, 2021). http://dx.doi.org/10.1007/s11274-021-03121-3.

Повний текст джерела

Анотація:

AbstractThe control of root-feeding wireworms has become more challenging as synthetic soil insecticides have been progressively phased out due to environmental risk concerns. Innovative microbial control alternatives such as the so-called attract-and-kill strategy depend on the rapid and successful development of dried encapsulated microorganisms, which is initiated by rehydration. Casein is a functional additive that is already used in food or pharmaceutical industry due to its water binding capacity. Cross-linked forms such as formalin-casein (FC), exhibit altered network structures. To determine whether FC influences the rehydration of alginate beads in order to increase the efficacy of an attract-and-kill formulation for wireworm pest control, we incorporated either casein or FC in different alginate/starch formulations. We investigated the porous properties of alginate/starch beads and subsequently evaluated the activities of the encapsulated entomopathogenic fungus Metarhizium brunneum and the CO2 producing yeast Saccharomyces cerevisiae. Adding caseins altered the porous structure of beads. FC decreased the bead density from (1.0197 ± 0.0008) g/mL to (1.0144 ± 0.0008) g/mL and the pore diameter by 31%. In contrast to casein, FC enhanced the water absorbency of alginate/starch beads by 40%. Furthermore, incorporating FC quadrupled the spore density on beads containing M. brunneum and S. cerevisiae, and simultaneous venting increased the spore density even by a factor of 18. Moreover, FC increased the total CO2 produced by M. brunneum and S. cerevisiae by 29%. Thus, our findings suggest that rehydration is enhanced by larger capillaries, resulting in an increased water absorption capacity. Our data further suggest that gas exchange is improved by FC. Therefore, our results indicate that FC enhances the fungal activity of both fungi M. brunneum and S. cerevisiae, presumably leading to an enhanced attract-and-kill efficacy for pest control. Graphic abstract

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Khalil, Saif, and Wei Sun. "Bioprinting Endothelial Cells With Alginate for 3D Tissue Constructs." Journal of Biomechanical Engineering 131, no. 11 (October 16, 2009). http://dx.doi.org/10.1115/1.3128729.

Повний текст джерела

Анотація:

Advanced solid freeform fabrication (SFF) techniques have been an interest for constructing tissue engineered polymeric scaffolds because of its repeatability and capability of high accuracy in fabrication resolution at the scaffold macro- and microscales. Among many important scaffold applications, hydrogel scaffolds have been utilized in tissue engineering as a technique to confide the desired proliferation of seeded cells in vitro and in vivo into its architecturally porous three-dimensional structures. Such fabrication techniques not only enable the reconstruction of scaffolds with accurate anatomical architectures but also enable the ability to incorporate bioactive species such as growth factors, proteins, and living cells. This paper presents a bioprinting system designed for the freeform fabrication of porous alginate scaffolds with encapsulated endothelial cells. The bioprinting fabrication system includes a multinozzle deposition system that utilizes SFF techniques and a computer-aided modeling system capable of creating heterogeneous tissue scaffolds. The manufacturing process is biologically compatible and is capable of functioning at room temperature and relatively low pressures to reduce the fluidic shear forces that could deteriorate biologically active species. The deposition system resolution is 10 μm in the three orthogonal directions XYZ and has minimum velocity of 100 μm/s. The ideal concentrations of sodium alginate and calcium chloride were investigated to determine a viable bioprinting process. The results indicated that the suitable fabrication parameters were 1.5% (w/v) sodium alginate and 0.5% (w/v) calcium chloride. Degradation studies via mechanical testing showed a decrease in the elastic modulus by 35% after 3 weeks. Cell viability studies were conducted on the cell encapsulated scaffolds for validating the bioprinting process and determining cell viability of 83%. This work exhibits the potential use of accurate cell placement for engineering complex tissue regeneration using computer-aided design systems.

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Liang, Hongtao, Jiankang He, Jinke Chang, Bing Zhang, and Dichen Li. "Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs." International Journal of Bioprinting 4, no. 1 (November 21, 2017). http://dx.doi.org/10.18063/ijb.v4i1.127.

Повний текст джерела

Анотація:

Cell printing has found wide applications in biomedical fields due to its unique capability in fabricating living tissue constructs with precise control over cell arrangements. However, it is still challenging to print cell-laden 3D structures simultaneously with high resolution and high cell viability. Here a coaxial nozzle-assisted electrohydrodynamic cell printing strategy was developed to fabricate living 3D cell-laden constructs. Critical process parameters such as feeding rate and stage moving speed were evaluated to achieve smaller hydrogel filaments. The effect of CaCl2 feeding rate on the printing of 3D alginate hydrogel constructs was also investigated. The results indicated that the presented strategy can print 3D hydrogel structures with relatively uniform filament dimension (about 80 μm) and cell distribution. The viability of the encapsulated cells was over 90%. We envision that the coaxial nozzle-assisted electrohydrodynamic printing will become a promising cell printing strategy to advance biomedical innovations.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Lee, Sangho, Min Kyung Lee, Hyunjoon Kong, and Young-sup Yoon. "Abstract 558: Microchanneled Polysaccharide Hydrogel Laden With Endothelial Cells and Mesenchymal Stem Cells With VEGF Facilitate Formation of Vascular Structures and Are Effective for Repairing Tissue Ischemia." Arteriosclerosis, Thrombosis, and Vascular Biology 34, suppl_1 (May 2014). http://dx.doi.org/10.1161/atvb.34.suppl_1.558.

Повний текст джерела

Анотація:

Various hydrogels are used to create vascular structure in vitro or to improve cell engraftment to overcome low cell survival in vivo, a main hurdle for bare cell therapy Recently we developed a modified alginate hydrogel within which microchannels are aligned to guide the direction and spatial organization of loaded cells. We investigated whether these cell constructs in which HUVECs and human mesenchymal stem cells (hMSCs) are co-loaded in this novel microchanneled hydrogel facilitate formation of vessels in vitro and in vivo, and enhance recovery of hindlimb ischemia. We crafted a modified alginate hydrogel which has microchannels, incorporates a cell adhesion peptide RGD, and was encapsulated with VEGF. We then compared vascular structure formation between the HUVEC only (2 x 105 cells) group and the HUVEC plus hMSC group. In the HUVEC+hMSC group, we mixed HUVECs and hMSCs at the ratio of 3:1. For cell tracking, we labeled HUVECs with DiO, a green fluorescence dye. After loading cells into the microchannels of the hydrogel, these constructs were cultured for seven days and were examined by confocal microscopy. In the HUVEC only group, HUVECs stands as round shaped cells without forming tubular structures within the hydrogel. However, in the HUVEC+hMSC group, HUVECs were stretched out and connected with each other, and formed vessel-like structure following pre-designed microchannels. These results suggested that hMSCs play a critical role for vessel formation by HUVECs. We next determined their in vivo effects using a mouse hindlimb ischemia model. We found that engineered HUVEC+hMSC group showed significantly higher perfusion over 4 weeks compared to the engineered HUVEC only group or bare cell (HUVEC) group. Confocal microscopic analysis of harvested tissues showed more robust vessel formation within and outside of the cell constructs and longer term cell survival in HUVEC+hMSC group compared to the other groups. In conclusion, this novel microchanneled alginate hydrogel facilitates aligned vessel formation of endothelial cells when combined with MSCs. This vessel-embedded hydrogel constructs consisting of HUVECs and MSCs contribute to perfusable vessel formation, prolong cell survival in vivo, and are effective for recovering limb ischemia.

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Ozbolat, Ibrahim T., and Bahattin Koc. "Modeling of Spatially Controlled Biomolecules in Three-Dimensional Porous Alginate Structures." Journal of Medical Devices 4, no. 4 (November 3, 2010). http://dx.doi.org/10.1115/1.4002612.

Повний текст джерела

Анотація:

This paper presents a computer-aided design (CAD) of 3D porous tissue scaffolds with spatial control of encapsulated biomolecule distributions. A localized control of encapsulated biomolecule distribution over 3D structures is proposed to control release kinetics spatially for tissue engineering and drug release. Imaging techniques are applied to explore distribution of microspheres over porous structures. Using microspheres in this study represents a framework for modeling the distribution characteristics of encapsulated proteins, growth factors, cells, and drugs. A quantification study is then performed to assure microsphere variation over various structures under imaging analysis. The obtained distribution characteristics are mimicked by the developed stochastic modeling study of microsphere distribution over 3D engineered freeform structures. Based on the stochastic approach, 3D porous structures are modeled and designed in CAD. Modeling of microsphere and encapsulating biomaterial distribution in this work helps develop comprehensive modeling of biomolecule release kinetics for further research. A novel multichamber single nozzle solid freeform fabrication technique is utilized to fabricate sample structures. The presented methods are implemented and illustrative examples are presented in this paper.

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Micaelo, R., A. C. Freire, and G. Pereira. "Asphalt self-healing with encapsulated rejuvenators: effect of calcium-alginate capsules on stiffness, fatigue and rutting properties." Materials and Structures 53, no. 1 (February 2020). http://dx.doi.org/10.1617/s11527-020-1453-7.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

Статті в журналах з теми "Alginate-encapsulated structures"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями