Готові списки джерел за темами / Agroinfiltratio

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Добірка наукової літератури з теми "Agroinfiltratio"

Автор: Grafiati

Опубліковано: 21 грудня 2024

Оновлено: 31 липня 2025

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Статті в журналах з теми "Agroinfiltratio"

Liu, Pei-Feng, Yanhan Wang, Robert G. Ulrich, et al. "Leaf-Encapsulated Vaccines: Agroinfiltration and Transient Expression of the AntigenStaphylococcal EndotoxinB in Radish Leaves." Journal of Immunology Research 2018 (2018): 1–9. http://dx.doi.org/10.1155/2018/3710961.

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Transgene introgression is a major concern associated with transgenic plant-based vaccines. Agroinfiltration can be used to selectively transform nonreproductive organs and avoid introgression. Here, we introduce a new vaccine modality in which Staphylococcal enterotoxin B (SEB) genes are agroinfiltrated into radishes (Raphanw sativusL.), resulting in transient expression and accumulation of SEBin planta. This approach can simultaneously express multiple antigens in a single leaf. Furthermore, the potential of high-throughput vaccine production was demonstrated by simultaneously agroinfiltrati

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Ambrós, Silvia, Choaa El-Mohtar, Susana Ruiz-Ruiz, et al. "Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana." Molecular Plant-Microbe Interactions® 24, no. 10 (2011): 1119–31. http://dx.doi.org/10.1094/mpmi-05-11-0110.

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Citrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues. Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable genetic system. A full-genome cDNA of CTV isolate T36 was cloned in binary plasmids and was used to agroinfiltrate Nicotiana benthamiana leaves, with or without coinfiltration with plasmids expressing different silencing-suppressor proteins. A time course analysis in agroinfiltrated leaves indicated that CTV accumulates and moves cell-to-cell fo

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Wang, Zhiquan, Xiaoyang Xu, Longjie Ni, Jinbo Guo, and Chunsun Gu. "Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus." PeerJ 7 (August 12, 2019): e7505. http://dx.doi.org/10.7717/peerj.7505.

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Background Hibiscus hamabo Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation H. hamabo is currently inefficient and laborious, restricting gene functional studies on this species. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies. Methods In this study, we tested the efficiency of a tobacco rattle virus vector in silencing the cloroplastos alterados 1 (CLA1) gene through agroinfilt

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Bridgeland, Aya, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, and Endang M. Septiningsih. "Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene." PLOS ONE 18, no. 4 (2023): e0283837. http://dx.doi.org/10.1371/journal.pone.0283837.

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Cowpea (Vigna unguiculata) is a legume staple widely grown across Sub-Saharan Africa and other tropical and sub-tropical regions. Considering projected climate change and global population increases, cowpea’s adaptation to hot climates, resistance to drought, and nitrogen-fixing capabilities make it an especially attractive crop for facing future challenges. Despite these beneficial traits, efficient varietal improvement is challenging in cowpea due to its recalcitrance to transformation and long regeneration times. Transient gene expression assays can provide solutions to alleviate these issu

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Debler, Johannes W., Bernadette M. Henares, and Robert C. Lee. "Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts." Plant Cell Reports 40, no. 5 (2021): 805–18. http://dx.doi.org/10.1007/s00299-021-02671-y.

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Abstract Key message Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Abstract Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed. Nicotiana benthamiana is widely used for agroinfiltration and the char

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Dickinson, Christopher C., Alexandra J. Weisberg, and John G. Jelesko. "Transient Heterologous Gene Expression Methods for Poison Ivy Leaf and Cotyledon Tissues." HortScience 53, no. 2 (2018): 242–46. http://dx.doi.org/10.21273/hortsci12421-17.

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Poison ivy [Toxicodendron radicans (L.) Kuntz] is a widely recognized native plant species because of its production of urushiol, which is responsible for delayed contact dermatitis symptoms in humans. Poison ivy is predicted to become both more prevalent and more noxious in response to projected patterns of climate change. Future studies on poison ivy chemical ecology will require reverse genetics to investigate urushiol metabolism. A prerequisite for reverse genetic procedures is the introduction and expression of recombinant DNA into poison ivy tissues. Poison ivy leaves and cotyledons were

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Chong, Xinran, Yue Wang, Xiaoyang Xu, et al. "Efficient Virus-Induced Gene Silencing in Ilex dabieshanensis Using Tobacco Rattle Virus." Forests 14, no. 3 (2023): 488. http://dx.doi.org/10.3390/f14030488.

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Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing to its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. dabieshanensis is currently difficult, which restricts functional gene studies and molecular breeding research on this species. Virus-induced gene silencing (VIGS) is a powerful tool for determining gene functions in plants. The present study reports the first application of VIGS mediated by a tobacco rattle virus (TRV) vector in I. dabieshanensis. We tested the efficiency of the VIGS

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Chiba, Sotaro, Kamal Hleibieh, Alice Delbianco, et al. "The Benyvirus RNA Silencing Suppressor Is Essential for Long-Distance Movement, Requires Both Zinc-Finger and NoLS Basic Residues but Not a Nucleolar Localization for Its Silencing-Suppression Activity." Molecular Plant-Microbe Interactions® 26, no. 2 (2013): 168–81. http://dx.doi.org/10.1094/mpmi-06-12-0142-r.

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The RNA silencing-suppression properties of Beet necrotic yellow vein virus (BNYVV) and Beet soil-borne mosaic virus (BSBMV) cysteine-rich p14 proteins have been investigated. Suppression of RNA silencing activities were made evident using viral infection of silenced Nicotiana benthamiana 16C, N. benthamiana agroinfiltrated with green fluorescent protein (GFP), and GF-FG hairpin triggers supplemented with viral suppressor of RNA silencing (VSR) constructs or using complementation of a silencing-suppressor-defective BNYVV virus in Chenopodium quinoa. Northern blot analyses of small-interfering

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Tu, Liqin, Shuhua Wu, Danna Gao, Yong Liu, Yuelin Zhu, and Yinghua Ji. "Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus." Viruses 13, no. 6 (2021): 1050. http://dx.doi.org/10.3390/v13061050.

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Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequence analysis showed that ToMMV-LN contains 6399 nucleotides (nts) and is most closely related to a ToMMV Mexican isolate with a sequence identity of 99.48%. Next, an infectious cDNA clone of ToMMV was constructed by a homologous recombination approach. Both the model host N. benthamiana and the natur

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Sindarovska, Yana, and Mykola Kuchuk. "Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro." Plants 10, no. 10 (2021): 2187. http://dx.doi.org/10.3390/plants10102187.

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Plant molecular farming has a great potential to produce valuable proteins. Transient expression technology provides high yields of recombinant proteins in greenhouse-grown plants, but every plant must be artificially agroinfiltrated, and open greenhouse systems are less controlled. Here, we propose to propagate agrobacteria-free plants with high-efficient long-term self-replicated transient gene expression in a well-controlled closed in vitro system. Nicotiana benthamiana plant tissue culture in vitro, with transient expression of recombinant GFP, was obtained through shoot induction from lea

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Більше джерел

Дисертації з теми "Agroinfiltratio"

Abe, Valeria Yukari. "Expressão transiente de genes de Phakopsora pachyrhizi em genótipos resistentes de soja visando a identificação de genes de avirulência." Universidade Federal de Viçosa, 2012. http://locus.ufv.br/handle/123456789/4404.

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Made available in DSpace on 2015-03-26T13:37:48Z (GMT). No. of bitstreams: 1 texto completo.pdf: 992783 bytes, checksum: 865bebc902b66a9a2f40688ff9d50a96 (MD5) Previous issue date: 2012-02-17 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Brazil is the second world soybean producer. Currently, the main limiting factor in this crop production is the Asian soybean rust (ASR) whose etiologic agent is the fungus Phakopsora pachyrhizi. The rust fungi are obligate parasites that during their interaction with the plant, they secrete effector proteins that manipulate host metaboli

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Tah, Tapashree Schoelz James E. "Chloroplast GFP expression in tobacco plants agroinfiltrated with tobacco mosaic virus based vectors." Diss., Columbia, Mo. : University of Missouri--Columbia, 2009. http://hdl.handle.net/10355/6604.

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Title from PDF of title page (University of Missouri--Columbia, viewed on Feb 19, 2010). The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file. Thesis advisor: Dr. James E. Schoelz. Includes bibliographical references.

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Joh, Lawrence Day. "High-level transient expression, extraction, and purification of recombinant [beta]-glucuronidase from agroinfiltrated lettuce /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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Thesis (Ph. D.)--University of California, Davis, 2005. Degree granted in Biological Systems Engineering. On t.p. "[beta]" appears as Greek letter. Also available via the World Wide Web. (Restricted to UC campuses)

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Bunwaree, Heemee Devi. "Implementation of a genetic screen for the identification of resistances to beet virus yellows." Electronic Thesis or Diss., Strasbourg, 2024. http://www.theses.fr/2024STRAJ042.

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Depuis l'interdiction des néonicotinoïdes dans l'Union Européenne, la production de betteraves sucrières est fortement menacée par des épidémies de jaunisses virales. Ces maladies sont causées par un complexe de plusieurs virus transmis par les pucerons. Parmi eux, les polérovirus, responsables de jaunisses modérées, tels que le beet mild yellowing virus (BMYV) et le beet chlorosis virus (BChV), sont particulièrement répandus. Afin d’améliorer le criblage des variétés de betteraves sucrières résistantes ou tolérantes aux jaunisses virales, nous avons mis au point un virus recombinant provoquan

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Bandyopadhyay, Amrita. "Analysis of the Arabidopsis Polyadenylation Factors PAP1, CstF64 and CstF77 and their characteristic inter-relationship." UKnowledge, 2009. http://uknowledge.uky.edu/gradschool_theses/601.

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3’-end modification by polyadenylation is a ubiquitous feature of almost all eukaryotic mRNA species and is catalyzed by a consortium of enzymes, the polyadenylation factors. Poly(A) polymerase (PAP), the enzyme catalyzing the addition of adenosine residues during the polyadenylation stage, exists in four isoforms within Arabidopsis. In silico and yeast two-hybrid studies showed that PAP1 has unique expression and interaction pattern in Arabidopsis, suggesting non-canonical functions of PAP1. Its exclusive interaction with PAP4 has not been reported in other living systems until now and hints

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Lin, Yi-Jyun, and 林怡君. "Examination Effect of Accessory Protein in T-DNA transferring by Transient Agroinfiltration of Tobacco." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/82128429955580164605.

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碩士 國立中興大學 生物科技學研究所 99 For the Agrobacterium-mediated plant transformation, the long journey for T-DNA transferring from bacteria till the final destination not only requires bacterial proteins, such as VirE2, VirE3, etc., but also is aided by various plant factors, including BTI1/BTI2/BTI3/Rab8, VIP1, VIP2, Ku70/Ku80, H2A, etc. Many reports had demonstrated that T1 progenies of transgenic Arabidopsis overproducing various accessory proteins exhibited higher transformation efficiency. Gene orthologs encoding the accessory proteins were isolated from rice and designated as “ET genes”

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Lin, Jia-Ying, and 林佳螢. "Development of an in Planta System to Monitor Phosphorus Status by Agroinfiltration and Agroinjection." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/21081124561212623300.

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碩士 國立臺灣大學 園藝暨景觀學系 102 Phosphorous (P), one of the essential mineral nutrients for plant growth and development, is involved in the regulation of several physiological and biochemical processes in plants. In our study, it was found that, in the tobacco and tomato plants at vegetative growth stage under phosphorus deficiency treatment, the phosphate (Pi) concentration in shoot and root decreased rapidly, the chlorophyll fluorescence (Fv/Fm), chlorophyll content and shoot fresh weight decreased relatively slowly, while the root/shoot fresh weight ratio increased gradually. Furthermore

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Ribeiro, Diana Margarida da Costa. "Optimization of transient expression procedures in Catharanthus roseus and Arabidopsis thaliana for subcellular localization studies." Master's thesis, 2010. http://hdl.handle.net/1822/15963.

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Dissertação de mestrado em Biotecnologia e Bio-Empreendedorismo em Plantas Aromáticas e Medicinais Nowadays, the availability of much gene sequence information demands the development of tools for their fast characterization at the protein level, where function actually resides. Here, the interest in the characterization of certain of the known Arabidopsis class III peroxidase (Prx) genes, as well as the interest in the characterization of candidate genes implicated in the metabolism of the anticancer terpenoid indole alkaloids of Catharanthus roseus, has led to the need of establishin

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Atnaseo, Chuthamat. "Transgenic Plant and Fungal Expression to Assay in vitro and in planta Activity of Sus scrofa beta-Defensin 1 and Nicotiana tabacum Defensin 1." Thesis, 2011. http://hdl.handle.net/10214/3193.

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To explore the use of defensins for transgenic plant disease resistance, expression by agroinfiltration of plants, stable transformation of plants and stable transformation of yeast were tested for porcine β-defensin 1 (pbd-1) and Nicotiana tabacum defensin 1 (Ntdef1). Attempts to screen constructs by agroinfiltration of Nicotiana benthamiana leaves revealed that agroinfiltration alone induced localized resistance against Colletotrichum destructivum. A comparison of Agrobacterium tumefaciens strains showed that the induced resistance required the transfer of type IV effectors into plant cells

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Peng, Hsien-Chieh, and 彭宣傑. "Study on the relatedness of LsGRP1 to the salicylic acid-induced resistance of Lilium cv. Star Gazer by agroinfiltration." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/77579617912117147541.

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碩士 國立臺灣大學 植物病理與微生物學研究所 94 Lilium is a bulbous crop, severely affected by a major fungal disease, gray mold, caused by Botrytis elliptica. As know, development of necrotic lesions in the leaves of Oriental lily cultivar Star Gazer could be suppressed by the application of salicylic acid (SA). A cDNA sequence, named LsGRP1, coding for a 138-amino acid protein has been identified via suppression subtractive hybridization and differential screening. When the lily plants were treated with SA solution, the amount of LsGRP1 transcript analyzed by Northern blot hybridization increased. In

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Частини книг з теми "Agroinfiltratio"

Spiegel, Holger, Stefan Schillberg, and Greta Nölke. "Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression." In Recombinant Proteins in Plants. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2241-4_6.

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AbstractThe agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type plants as biomass, agroinfiltration exploits the ability of plants to synthesize even complex multimeric proteins that require oxidative folding and/or post-translational modifications, while avoiding the expensive and time-consuming creation of stably transformed plant lines. Here we describe a generic method for the transient expression of recombinant proteins in Nicotiana benthamiana at the small to medium laboratory scale, including appropr

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Helm, Jutta Maria, Elena Dadami, and Kriton Kalantidis. "Local RNA Silencing Mediated by Agroinfiltration." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-123-9_7.

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Liu, Lijing, Qingzhen Zhao, and Qi Xie. "In Vivo Ubiquitination Assay by Agroinfiltration." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-809-2_12.

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Chen, Qiang, Matthew Dent, Jonathan Hurtado, et al. "Transient Protein Expression by Agroinfiltration in Lettuce." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3289-4_4.

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D'Aoust, Marc-André, Pierre-Olivier Lavoie, Julie Belles-Isles, Nicole Bechtold, Michèle Martel, and Louis-P. Vézina. "Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration." In Recombinant Proteins From Plants. Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-407-0_3.

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Von Lanken, Carol, and Arthur G. Hunt. "Transient Expression Using Agroinfiltration to Study Polyadenylation in Plants." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-2175-1_11.

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Picard, Kelsey, Robyn Lee, Roger Hellens, and Richard Macknight. "Transient Gene Expression in Medicago truncatula Leaves via Agroinfiltration." In Legume Genomics. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-613-9_15.

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Menassa, Rima, Adil Ahmad, and Jussi J. Joensuu. "Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming." In Molecular Farming in Plants: Recent Advances and Future Prospects. Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-2217-0_9.

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Cournoyer, Patrick, and S. P. Dinesh-Kumar. "Studying NB-LRR Immune Receptor Localization by Agroinfiltration Transient Expression." In Methods in Molecular Biology. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61737-998-7_1.

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Vaghchhipawala, Zarir, Clemencia M. Rojas, Muthappa Senthil-Kumar, and Kirankumar S. Mysore. "Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-682-5_6.

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