Дисертації з теми "AGC REGULATORS"
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SBANO, LUIGI. "TFEB and AGC1 as homeostasis regulators: soce modulation and cell proliferation control." Doctoral thesis, Università degli studi di Ferrara, 2018. http://hdl.handle.net/11392/2478708.
Повний текст джерелаThis thesis focused on two different proteins and their involvement in cellular homeostasis: the transcription factor EB (TFEB) and the mitochondrial aspartate-glutamate carrier isoform 1 (AGC1). The first work (chapter 1) presented is aimed to investigate the roles of TFEB and lysosomes in intracellular Ca2+ homeostasis. As well documented, lysosomes are membrane-bound organelles mainly involved in catabolic processes. In addition, lysosomes can expel their contents outside of the cell via lysosomal exocytosis. Some of the key steps involved in these important cellular processes, such as vesicular fusion and trafficking, require calcium (Ca2+) signaling. Recent data show that lysosomal functions are transcriptionally regulated by transcription factor EB (TFEB) through the induction of genes involved in lysosomal biogenesis and exocytosis. We studied the effect of transient modulation of TFEB expression in HeLa cells by measuring the cytosolic Ca2+ response after capacitative Ca2+ entry activation and Ca2+ dynamics in the endoplasmic reticulum (ER) and directly in lysosomes. Our observations show that transient TFEB overexpression significantly reduces cytosolic Ca2+ levels under a capacitative influx model and ER re-uptake of calcium, increasing the lysosomal Ca2+ buffering capacity. Moreover, lysosomal destruction or damage abolishes these TFEB-dependent effects in both the cytosol and ER. These results suggest a possible Ca2+ buffering role for lysosomes and shed new light on lysosomal functions during intracellular Ca2+ homeostasis. The second work (chapter 2) is focused on AGC1, the isoform 1 of the mitochondrial aspartate-glutamate carrier which catalyzes a Ca2+-stimulated export of aspartate to the cytosol in exchange for glutamate, and is a key component of the malate-aspartate shuttle which transfers NADH reducing equivalents from the cytosol to mitochondria. By sustaining the complete glucose oxidation, AGC1 is thought to be important in providing energy for cells, in particular in the CNS and muscle where this protein is mainly expressed. Defects in the AGC1 gene cause AGC1 deficiency, an infantile encephalopathy with delayed myelination and reduced brain N-acetylaspartate (NAA) levels, the precursor of myelin synthesis in the CNS. Here, we show that undifferentiated Neuro2A cells with down-regulated AGC1 display a significant proliferation deficit associated with reduced mitochondrial respiration, and are unable to synthesize NAA properly. In the presence of high glutamine oxidation, cells with reduced AGC1 restore cell proliferation, although oxidative stress increases and NAA synthesis deficit persists. Our data suggest that the cellular energetic deficit due to AGC1 impairment is associated with inappropriate aspartate levels to support neuronal proliferation when glutamine is not used as metabolic substrate, and we propose that delayed myelination in AGC1 deficiency patients could be attributable, at least in part, to neuronal loss combined with lack of NAA synthesis occurring during the nervous system development.
Ruiz, Carrasco Karina Beatriz <1972>. "Expression profiling in developing peach seed and mesocarp as affected by growth regulators." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4361/1/RuizCarrasco_KarinaBeatriz_Tesi.pdf.
Повний текст джерелаRuiz, Carrasco Karina Beatriz <1972>. "Expression profiling in developing peach seed and mesocarp as affected by growth regulators." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4361/.
Повний текст джерелаRIBERA, A. MIRANDA. "AGP REGULATES THE NEUTROPHIL AND ENDOTHELIAL CELL INFLAMMATORY RESPONSE." Doctoral thesis, Università degli Studi di Milano, 2010. http://hdl.handle.net/2434/150165.
Повний текст джерелаMalgorzata, Anna Carran. "Children and gambling : attitudes, behaviour, harm prevention and regulatory responses." Thesis, Queen Mary, University of London, 2015. http://qmro.qmul.ac.uk/xmlui/handle/123456789/12576.
Повний текст джерелаGiedt, Michelle Suzanne. "JAK/STAT SIGNALING REGULATES GAMETOGENESIS AND AGE-RELATED REPRODUCTIVE MAINTENANCE." UKnowledge, 2018. https://uknowledge.uky.edu/biology_etds/52.
Повний текст джерелаLeishman, David <1968>. "International Agricultural Trade under Regulatory Asymmetry: An Economic Analysis of SME Export Behavior." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4939/1/Leishman_David_Tesi.pdf.
Повний текст джерелаLeishman, David <1968>. "International Agricultural Trade under Regulatory Asymmetry: An Economic Analysis of SME Export Behavior." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4939/.
Повний текст джерелаBosson, Andrew D. (Andrew David). "Modulation of Ago-miRNA regulatory networks by cis-sequence elements and target competition." Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/89938.
Повний текст джерелаVita. Cataloged from PDF version of thesis.
Includes bibliographical references.
regulators of gene expression in a wide range of organisms and biological processes. Each miRNA guides Argonaute (Ago) protein complexes to target and repress hundreds of genes in a sequence-dependent manner. To identify all targets of miRNA regulation, we performed UV crosslinking and immunoprecipitation (CLIP) of Ago complexes in mouse embryonic (ESC) and mesenchymal (MSC) stem cell lines. We also captured the genome-wide miRNA-independent binding footprint of Ago by performing CLIP in cells that lack Dicer, an enzyme required for mature miRNA biogenesis. We surprisingly found that Ago bound a similar set of genes in the absence of Dicer, and this overlap in target genes was due partially to residual, unprocessed miRNAs in the Dicer KO cells. Other potential sites of miRNA-independent Ago interactions, such as histone transcripts and poly-A cleavage and polyadenylation sites, were also identified. One Ago CLIP dataset revealed the enrichment for a G-rich sequence motif at Ago target sites. We later demonstrated that the G-motif is not directly bound to Ago but rather is enriched near miRNA-guided Ago binding sites. Its presence near miRNA target sites is associated with stronger repression of Ago-miRNA targets. Fortuitously, the original Ago CLIP dataset that identified the G-motif was later shown to likely contain target sites of another co-immunoprecipitating RNA binding protein (RBP). Using mass spectroscopy of Ago antibody immunoprecipitations from Ago KO cells, we identified a list of interacting RBPs that could potentially augment Ago-miRNA activity through the G-motif. To investigate target competition in miRNA networks, we related our CLIP analysis of genome-wide, quantitative Ago binding to measurements of absolute miRNA and target RNA concentrations. We found that all miRNAs other than the miR-290 family in ESCs and let-7 family in MSCs were expressed at concentrations below their total target pool. However, 8-12 miRNA families were expressed at near or greater than equimolar ratios with their pool of high affinity targets, and this affinity-partitioned stoichiometry led to significant Ago accumulation and repression of high affinity target sites despite little consequential binding at low affinity sites. Single-cell reporter assays demonstrated that high expressed miRNAs are not susceptible to physiological inductions of competing target transcripts but targets of lower expressed miRNAs are derepressed in a weakly threshold-like manner upon increased target pool levels. In summary, we identify a network of confidently bound targets of miRNA regulation in ESCs and MSCs, reveal the extent of miRNA-independent binding in these two cell types, provide a list of potential miRNA enhancer RBPs, and create a quantitative context for evaluating target competition in miRNA networks.
by Andrew D. Bosson.
Ph. D.
Thoendel, Matthew James. "Synthesis of the accessory gene regulator autoinducing peptide in Staphylococcus aureus." Diss., University of Iowa, 2012. https://ir.uiowa.edu/etd/2999.
Повний текст джерелаKuswanto, Wilson F. "The Regulatory T Cell Response to Skeletal Muscle Injury and Its Decline With Age." Thesis, Harvard University, 2015. http://nrs.harvard.edu/urn-3:HUL.InstRepos:23845413.
Повний текст джерелаMedical Sciences
Zahoor, Muhammad kashif. "Genome wide analysis for novel regulators of growth and lipid metabolism in drosophila melanogaster." Phd thesis, Université Paris Sud - Paris XI, 2011. http://tel.archives-ouvertes.fr/tel-00664844.
Повний текст джерелаScholz, Sebastian [Verfasser], Kay H. [Akademischer Betreuer] Schneitz, Kay H. [Gutachter] Schneitz, and Brigitte [Gutachter] Poppenberger-Sieberer. "Regulatory feedback between AGC kinases coordinating Arabidopsis ovule integument growth / Sebastian Scholz ; Gutachter: Kay H. Schneitz, Brigitte Poppenberger-Sieberer ; Betreuer: Kay H. Schneitz." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1185637672/34.
Повний текст джерелаOcariza, Linnette Mae. "Polyphosphate : a novel negative regulator of complement and its therapeutic potential in age-related macular degeneration." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/55196.
Повний текст джерелаMedicine, Faculty of
Pathology and Laboratory Medicine, Department of
Graduate
Rimington, Tracy L. "Expression, purification and characterisation of the Cystic Fibrosis Transmembrane conductance Regulator (CFTR) in Saccharomyces cerevisiae." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/expression-purification-and-characterisation-of-the-cystic-fibrosis-transmembrane-conductance-regulator-cftr-in-saccharomyces-cerevisiae(5c8c606b-8925-4627-91dc-67a896b9f286).html.
Повний текст джерелаMcCulloch, John Anthony. "Avaliação da funcionalidade do locus acessory gene regulator (agr) em cepas de «Staphylococcus aureus» brasileiras com suscetibilidade reduzida aos glicopeptídeos." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-04042007-153429/.
Повний текст джерелаThe treatment of staphylococcal infections has lately been a strenuous undertaking due to the resistance of Staphylococcus aureus to multiple antibiotics. The antimicrobial drug of choice for the treatment of methicillin resistant S. aureus (MRSA) is the glycopeptide vancomycin. Since the first isolation of S. aureus with reduced susceptibility to vancomycin (VISA) in 1997, there has been growing concern as to the dissemination of this resistance phenotype among isolates of this species. The molecular mechanisms that result in low level resistance to vancomycin have not yet been completely elucidated. The correct detection of this phenotype in the clinical laboratory is tricky, for the techniques available for this purpose are hard to execute and interpret. Until now, lateral transmission (dissemination) of VISA has not been reported and all strains bearing this phenotype have been isolated from patients who had been making prolonged use of vancomycin. A deficiency in the accessory gene regulator (agr) has been proposed as a risk factor for the acquisition of a VISA phenotype by a susceptible strain. For this study, 47 nosocomial VISA strains, that had been isolated in another study, were used. These strains were isolated from multiple geographical regions of Brazil, and included 5 VISA strains. The minimal inhibitory concentrations (MIC) of vancomycin and oxacillin, as well as haemolysis in sheep and rabbit agar, adhesion to polystyrene and agr polymorphism were determined in all of these strains. The integrity of the agr locus was determined by PCR-RFLP and by nucleotide sequencing in a sample of 13 strains chosen to be representative of the 47 strains studied. The integrity of the Staphylococcal accessory regulator sarA was also determined by nucleotide sequencing in these 13 strains. Another representative sample of 18 strains that were susceptible to vancomycin were submitted to induction of resistance to vancomycin by serial passage in increasing concentrations of this drug. The mutation rate of a mutation that leads to the ability of growing in a concentration of 6 µg/mL of vancomycin was determined for 8 strains by fluctuation assays. There was no correlation between the acquisition of resistance to vancomycin with either haemolysis or adhesion to polystyrene. Most strains (82.9%) bore a group I agr polymorphism, including all of the VISA strains. Two strains could not be induced to resistance. The time taken for each strain to acquire resistance to vancomycin did not correlate with any phenotypic or genotypic characteristic pertaining to a group of strains. The rate of mutation that leads to the ability of growing in 6µg/mL of vancomycin proved to be higher for a strain belonging to the Brazilian Endemic Clone (BEC) bearing an agr group I polymorphism, and did not vary according to presence or type of agr locus. Only one of the VISA strains presented a mutation in the agr locus that renders it disfunctional. The agr loci of the other strains studied presented themselves to be intact. The sarA loci of the strains evaluated were intact however presented functional polymorphisms that were groups according to the clonal lineage of the strains. It can thus be concluded that the functional integrity of the agr locus is not a sine qua non condition for the acquisition of low level resistance to vancomycin by a susceptible strain. Bearing of an agr group II polymorphism does not predispose a strain to acquire resistance to vancomycin, as has been previously suggested in literature.
Qian, Jiajing. "Effects of Hordeum vulgare and Hordeum bulbosum genotypes, seed age, culture methods and plant growth regulators on barley haploid production." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56678.
Повний текст джерелаBassani, Milena Tomasi. "Caracterização de grupos agr e sua relação com perfil enterotoxigênico e antimicrobiano em Staphylococcus aureus isolados de diferentes origens." Universidade Federal de Pelotas, 2009. http://repositorio.ufpel.edu.br/handle/ri/1312.
Повний текст джерелаhe accessory gene regulator (agr) is a S. aureus global regulator of virulence factors, as the staphylococcal enterotoxins (SE), responsible for the staphylococcal food poisoning. There are four different agr groups due to the polymorphism in the amino acids sequence of the agrC and agrD. In the literature is described a relationship among agr groups and virulence factors, diseases, preferential host and antibiotic resistance. In this context, it was aimed to characterize the agr groups through biplex PCR, and relationship among agr groups, enterotoxigenic and antimicrobian profiles of S. aureus isolated from foods and bovine mastitis milk. A total of 115 strains were used to characterize the agr groups , being 30 isolated from f bovine mastitis milk and 85 isolated from several sources of foods. To assess the relationship between agr groups and enterotoxin production were used 14/85 strains previously characterized for the presence of some enterotoxins (sea, seb, sec, sed e cluster egc). To determine the profile of antibiotics resistance were used 71/115 strains. We observed a prevalence of agr group II with19.1% (22/115 strains), followed by the agr I with 8.6% (10/115 strains), agr III with 7.8% (9 / 115 strains), and agr IV with6.0% (7 / 115 strains). Among the strains isolated from bovine mastitis milk agr group I was prevailed with 20% (6/30 strains), whereas in the strains isolated from several food sources was observed prevalence of agr group II with 32.7% (18/85 strains), especially among those from chicken meat. Among the 14 strains (14/85) that contained enterotoxin genes, the majority of them contained the cluster egc (70%) belonged to agr II, whereas no relationship was found with those who had the genes for the classical SE (sea, seb, sec, sed). Considering the antibiotic resistance 100% of bovine mastitis milk strains and from various sources of food were resistant to penicillin, ampicillin, cefoxitin, and vancomycin. Relationship was observed between food strains, which were resistant to vancomycin and agr II, however, no relationship was found between antibiotic profile and agr groups among the strains isolated from bovine mastitis milk. These results demonstrated the prevalence of agr II among food strains and agr I among bovine mastitis milk strains. Moreover, the strains that carried the cluster egc were predominant agr II, which could indicate the occurrence of a clonal group among those. Another important result obtained in this study was the high rate of S. aureus multiresistant strains isolated from food, which emphasizes the importance of dissemination of these strains among foods.
O accessory gene regulator (agr) é um regulador global de fatores de virulência em S. aureus, como as enterotoxinas estafilocócicas (EE), responsáveis pela intoxicação alimentar estafilocócica. São conhecidos quatro distintos grupos agr devido ao polimorfismo na seqüência dos aminoácidos de agrC e agrD. Na literatura descreve-se relação entre fatores de virulência, patogenias, hospedeiro preferencial e perfil de resistência a antibióticos com grupos agr. Neste contexto, objetivou-se caracterizar grupos agr através de biplex PCR, e relacioná-los com os perfis enterotoxigênico e antimicrobiano de cepas de S. aureus isoladas em alimentos e leite de vacas mastíticas. Para caracterização dos grupos agr foram utilizadas 115 cepas, sendo 30 isoladas em leite de vacas mastíticas e 85 em diversas fontes de alimentos. Para a relação entre grupos agr e produção de enterotoxina foram utilizadas 14/85 cepas previamente caracterizadas quanto à presença de alguma enterotoxina (eea, eeb, eec, eed e cluster egc), já para determinar o perfil de resistência a antibióticos utilizaram-se 71/115 cepas. Observou-se uma prevalência do grupo agr II, com 19,1% (22/115 cepas), seguido do agr I, com 8,6% (10/115 cepas), agr III 7,8% (9/115 cepas), e agr IV, 6,0% (7/115 cepas). Entre as cepas isoladas em leite de vacas com mastite houve predomínio do grupo agr I, com 20% (6/30 cepas); já nas cepas isoladas de diversas fontes de alimentos observou-se prevalência do grupo agr II, com 32,7% (18/85 cepas), especialmente entre as provenientes de carne de frango. Entre as 14/85 cepas que carreavam genes de enterotoxinas, a maioria que albergava o cluster egc (70%), pertencia ao grupo agr II, enquanto nenhuma relação foi observada com aquelas que possuíam os genes para as EE clássicas (eea, eeb, eec, eed). Com relação ao perfil de resistência antimicrobiana, 100% das cepas isoladas de leite de vacas com mastite e das diversas fontes de alimentos apresentaram resistência à penicilina, ampicilina, cefoxitina e vancomicina. Observou-se relação entre cepas isoladas de alimentos, que eram resistentes à vancomicina e grupo agr II, entretanto, nenhuma relação foi observada entre perfil antimicrobiano e grupos agr entre as cepas isoladas em leite de vacas com mastite. Através destes resultados demonstra-se a prevalência do grupo agr II entre as cepas isoladas de alimentos e do grupo agr I em cepas isoladas de leite de vacas mastíticas. Além disso, nas cepas que carreiam o cluster egc houve predominância do grupo agr II, podendo indicar um grupo clonal entre essas. Outro resultado relevante obtido neste estudo foi à elevada taxa de cepas de S. aureus multiresistentes isoladas em alimentos, o que ressalta a importância da disseminação de cepas multiresistentes entre os alimentos.
Maloney, Shawn. "Dual role of SIRT1 as a regulator of retinal development and a therapeutic target in age-related macular degeneration." Thesis, McGill University, 2011. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=104689.
Повний текст джерелаLa dégénérescence maculaire liée à l'âge (DMLA) est la principale cause de cécité chez les personnes âgées dans les pays développés. Une recherche dynamique est en cours pour élucider des cibles moléculaires potentiels pour le traitement de la dégénérescence à la fois pour la forme atrophique et la forme néovasculaire de cette maladie. L'actuelle pharmacothérapie est efficace chez certains patients mais pas suffisante pour arrêter la progression de la maladie ou la réparation des dommages qui ont déjà eu lieu. La decouverte de nouveaux medicaments et le remplacement de cellules rétiniennes représentent les deux avenues les plus prometteuses de traitements possibles. Le but de cette thèse est d'étudier le rôle d'un régulateur récemment identifié du développement neuronal, SIRT1, dans le developpement de la retine et de rechercher si l'inhibition pharmacologique de cette protéine représente une option de traitement possible dans la DMLA. Via l'immunohistochimie et l'immunocytochimie, nous avons évalué l'expression et la localisation subcellulaire de SIRT1 et de son inhibiteur inné, DBC1, chez la souris et les humains dans les retines fœtales et adultes. Nous avons également étudié SIRT1 dans les cellules souches de la rétine chez la souris et l'homme. Nous avons trouvé SIRT1 largement exprimé dans la rétine en développement et des adultes et à un régulateur de gènes clés du développement de la rétine, à savoir PAX6, Nestin et CRX. En outre, nous avons constaté que les cellules précurseurs des photorécepteurs ont été parmi les plus petites cellules dans la population hétérogène de cellules progénitrices. Collectivement, ces résultats fournissent la base pour la manipulation de l'expression SIRT1 dans les petits progéniteurs rétiniens comme un moyen d'augmenter le rendement des photorécepteurs à la transplantation dans les modèles de dégénérescence rétinienne. Nous avons en outre constaté que SIRT1 est fortement exprimé dans les membranes néovasculaires humaines et avons cherché à inhiber son activité pharmacologique par le nicotinamide. Nous avons trouvé que Nicotinamide est un puissant régulateur de l'hypoxie et de l'angiogenèse au niveau de la protéine et de l'ARN. Ces résultats indiquent que l'inhibiteur de SIRT1, Nicotinamide est un agent possible pour le traitement de la DMLA néovasculaire. D'autres études de la nicotinamide devraient être poursuivit dans des modèles animaux de la DMLA. Au meilleur de notre connaissance, c'est la première fois qu'une analyse détaillée de SIRT1 l'identifie comme un régulateur du développement tant de la rétine et de la néovascularisation choroïdienne.
Zaffalon, Valerio. "Dissecting the transcriptional regulatory network of seed and mesocarp development in peach." Doctoral thesis, Università degli studi di Padova, 2014. http://hdl.handle.net/11577/3423828.
Повний текст джерелаIl pesco (Prunus persica) è uno dei più importanti alberi da frutto al mondo e la specie modello per le drupacee. Lo sviluppo del frutto di pesco è caratterizzato da un stretto rapporto tra il seme e il pericarpo durante i primi stadi, seguito negli stadi successivi da un disaccoppiamento nello schema di sviluppo dovuto alla lignificazione dell’endocarpo. Le varie cultivar di pesco possono avere dei periodi di sviluppo del frutto dalla lunghezza estremamente variabile, pur avendo un seme che si sviluppa in maniera simile. Per questo, comprendere la relazione tra seme e pericarpo può chiarire il meccanismo che regola lo sviluppo del frutto nel suo complesso. L’approccio transcrittomico è uno strumento potente per analizzare questa relazione, dato che produce un gran numero di informazioni sulla trascrizione di un gran quantitativo di geni in un singolo esperimento. Il Capitolo II consiste in un articolo pubblicato che descrive l’uso dell’array µPEACH1.0 nello studiare la relazione tra seme e mesocarpo e tra stadi iniziali e finali di sviluppo nella cultivar Fantasia. Campioni di mRNA di pesco sono stati raccolti dagli stadi iniziali e finali dei due organi e ibridizzati sulle 4 806 sonde dell’array µPEACH1.0. I dati trascrittomici ottenuti da questi campioni sono stati quindi confrontati. Sono stati trovati dei geni marcatori per i quattro stadi di sviluppo del pesco (Stadio S1: divisione ed espansione cellulare nel frutto, S2: lignificazione dell’endocarpo, S3: espansione cellulare nel mesocarpo, S4: maturazione) sia per il mesocarpo che per il seme e la loro espressione confermata con la qRT-PCR: I marcatori stadio-specifici per il mesocarpo sono rispettivamente per S1, S2, S3 e S4: una proteina RD22-like, una serin-carbossipeptidasi, una proteina correlata alla senescenza e una Aux/IAA; mentre per il seme sono, rispettivamente: una proteina trasportatrice di lipidi (LTP1), una proteina correlata alla patogenesi (PR), una prunina e una proteina LATE EMBRYOGENESIS ABUNDANT (LEA). La qRT-PCR ha confermato che questi geni sono marcatori anche in una cultivar precoce (SpringCrest) e in un genotipo a maturazione lenta (slr). Quindi i dati sono stati analizzati con lo strumento HORMONOMETER al fine di misurare indirettamente il quantitativo relativo di ormoni nei vari organi e stadi di sviluppo. E’ emerso che l’auxina, le citochinine e le gibberelline possono essere coinvolte nella segnalazione durante l’inizio dello sviluppo, quando vi è comunicazione tra i due organi. Il Capitolo III è un articolo non pubblicato nel quale viene descritto come venga utilizzata una nuova piattaforma microarray (µPEACH3.0) nello studiare lo sviluppo del seme e del mesocarpo di pesco. La recente pubblicazione del genoma di pesco ha permesso lo sviluppo di un microarray che copre l’intero genoma, superando così il problema di avere un array che misura l’espressione genica solo di una parte del genoma. Rispetto allo studio descritto nel Capitolo I, anche il numero di campioni è stato incrementato: sono state usate tre repliche biologiche per sei diversi momenti per ciascuno dei due organi, dando così una visuale più vasta sullo sviluppo di questi due tessuti. L’array µPEACH3.0 ha funzionato bene, dando una correlazione con i dati di qRT-PCR pari a 0.77, un numero simile a quello trovato per altri array. I dati trascrittomici hanno facilmente distinto i due tessuti e i sei campionamenti, come mostrato dall’analisi delle componenti principali. Il 69% delle sonde ha prodotto un segnale significativo in almeno uno dei campioni, ciò nonostante, considerando che il numero di sonde funzionanti decresce se si prende in considerazione un solo tessuto, è probabile che testando il microarray con mRNA proveniente da altri tessuti (come le foglie o le radici) aumenti il numero di segnali significativi provenienti dall’array. L’analisi globale dell’attività genica è stata indirizzata ai primi stadi di sviluppo. I dati hanno permesso di indentificare parecchi geni coinvolti nei processi del ciclo cellulare che si verificano all’inizio dello sviluppo sia del mesocarpo che del seme. In particolare, è stato trovate che geni della famiglia TITAN sono attivi nel seme contenente endosperma. L’analisi dei geni del ciclo cellulare nel mesocarpo ha mostrato l’esistenza di due diversi profili di espressione: mentre i geni relativi alla mitosi erano espressi solo nello stadio S1, i geni della replicazione del DNA hanno mostrato un doppio picco di espressione, in S1 e poi in S3/S4, suggerendo che in questi stadi possono verificarsi eventi di endoreduplicazione. Con l’utilizzo di qRT-PCR, i livelli d’esrpessione di questi geni sono stati testati anche in altre cultivar; i dati ottenuti suggeriscono che nel genotipo slr la mancanza di endoreduplicazione possa essere coinvolta nel basso tasso di crescita durante lo stadio S3 di questo genotipo. Sono stati quindi valutati i profili d’espressione di famiglie di fattori di trascrizione (FT), dato che si ritiene che i fattori di trascrizione siano le proteine con i ruoli più importanti nella regolazione durante lo sviluppo. E’ stato trovato che FT delle famiglia SQUAMOSA promoter Binding Protein (SBP) hanno un alto livello di espressione all’inizio dello sviluppo di entrambi gli organi considerati, il quale successivamente diminuisce velocemente. E’ stato scoperto che nel seme maturo è indotta la trascrizione di FT di tipo Growth-Regulating Factor (GRF). Questi dati sono stati confermati con l’utilizzo di qRT-PCR in ‘SpringCrest’ precoce e nel genotipo a lenta maturazione slr. Dato che in altre specie vegetali l’abbondanza dell’mRNA di geni appartenenti a queste famiglie di FT è regolata da microRNA (miRNA) specifici, è stata misurata l’espressione degli omologhi di pesco di questi miRNA. In tre diverse cultivar è stata trovata una correlazione negativa nel contenuto di RNA per le seguenti coppie microRNA/FT: miR156/SBP, miR396/GRF e mir167/ARF8, suggerendo non solo che questi miRNA posseggono la stessa attività un pesco, ma anche che i miRNA sono profondamente coinvolti nella rete regolativa sottostante lo sviluppo del frutto di pesco. In appendice vi è uno studio pubblicato nel quale viene descritto l’uso di µPEACH3.0 nello studiare gli effetti delle ferite su due cultivar con diversa tolleranza a questo stress. Sono stati utilizzati campioni di RNA estratti da mesocarpi feriti o intatti della cultivar “melting” Glohaven (GH) e della cultivar “slow melting” BigTop (BT). I dati trascrittomici, confermati dall’analisi tramite qRT-PCR, hanno mostrato il coinvolgimento di fattori di trascrizione di tipo WRKY, AP2/ERF, e HSP20 nella risposta di GH alla ferite. Insieme a questi, è stato trovato che nel mesocarpo ferito di GH viene indotta l’espressione anche di geni coinvolti nella risposta agli stress, nel metabolismo della parete cellulare, nella biosintesi dei fenilpropanoidi e triterpenoidi.
Kolar, Stacey Lynn. "The Role and Regulation of NsaRS: a Cell-Envelope Stress Sensing Two-Component System in Staphylococcus aureus." Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4104.
Повний текст джерелаGessler, Dominic J. "Age-dependent rAAV Mediated Reconstitution of hASPA Reveals N-acetylaspartate Regulates Fuel Selection in the Central Nervous System." eScholarship@UMMS, 2020. https://escholarship.umassmed.edu/gsbs_diss/1110.
Повний текст джерелаSasaki, Mayumi. "Cloning of ABCA17, a novel rodent sperm-specific ABC (ATP-binding cassette) transporter that regulates intracellular lipid metabolism." Kyoto University, 2007. http://hdl.handle.net/2433/135646.
Повний текст джерелаSchwartz, Brian David. "Deficiencies in regulations for anti-money laundering in a cyberlaundering age including COMET Central Online AML Merchant Enforcement Tool /." [Ames, Iowa : Iowa State University], 2009.
Знайти повний текст джерелаSt-Pierre, Renee 1979. "Retailer compliance with youth access statutes and regulatory policies for lottery products and alcohol : evaluating the role of gender and vendor age." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116064.
Повний текст джерелаPechtl, Isabell C. "Study of complement regulatory factor H based on Forster resonance energy transfer and investigation of disease-linked genetic variants." Thesis, University of Edinburgh, 2010. http://hdl.handle.net/1842/4721.
Повний текст джерелаSlingsby, Fern. "Investigating a C1QTNF5 mutation associated with macular degeneration." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/4220.
Повний текст джерелаHarthan, Laura Beth. "The Effect of Age and Nutrient Status on Growth Characteristics of Turkey Satellite Cells." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1375096848.
Повний текст джерелаChen, Jiahong. "Data protection in the age of Big Data : legal challenges and responses in the context of online behavioural advertising." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33149.
Повний текст джерелаGustot, Adelin. "Phosphatidylethanolamine regulates the structure and function of HorA, a bacterial multidrug transporter." Doctoral thesis, Universite Libre de Bruxelles, 2009. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210230.
Повний текст джерелаHorA is a multidrug transporter expressed in Lactobacillus brevis, a Gram-positive beer spoilage bacterium. It turned out that phosphatidylethanolamine (PE) was indispensable to maintain both the activity and the structural integrity of HorA.
Surprisingly, replacement of PE by the chemically related PC (phosphatidylcholine) did not led to the suppression of HorA activity, but to an unexpected phenotype. Whereas the cytoplasmic domains of HorA were still able to hydrolyze ATP, the membrane parts of the transporter were unable to use that energy to mediate substrate transport. Using several biophysical methods particularly adapted to the study of reconstituted systems, we showed that the structure of HorA is strongly altered by this lipid replacement. In particular, the structural organization of the transmembrane domains of the protein is strongly affected.
Doctorat en Sciences agronomiques et ingénierie biologique
info:eu-repo/semantics/nonPublished
Bull, Elaine Elizabeth. "Entering the new ICE age : exploring the impact of the Information and Consultation of Employees (ICE) Regulations 2004 in medium organisations." Thesis, University of Kent, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.534334.
Повний текст джерелаKampourakis, Ioannis [Verfasser]. "Dialectics of Transparency and Secrecy in the Information Age : The Role of Whistleblowing Legislation in the Regulatory Governance of Markets and in National Security / Ioannis Kampourakis." Berlin : Freie Universität Berlin, 2019. http://d-nb.info/1195081014/34.
Повний текст джерелаDonvito, Béatrice. "Caractérisation moléculaire de l'hémolysine secrétée par Staphylococcus lugdunensis : étude de sa distribution chez les autres staphylocoques et de sa régulation par le système AGR ("Accessory Gene Regulator")." Lyon 1, 1997. http://www.theses.fr/1997LYO1T038.
Повний текст джерелаPorcheron, Gaëlle. "Métabolisme d'un prébiotique par une souche d'escherichia coli pathogène : décryptage fonctionnel et mobilité de la région fos." Thesis, Tours, 2011. http://www.theses.fr/2011TOUR4033.
Повний текст джерелаThe fos region of the avian pathogenic Escherichia coli strain BEN2908 is involved in fructan metabolism, prebiotics widely used commercially in food products for both humans and animals. This metabolism contributes to the establishment of BEN2908 in its reservoir, the intestine. The fos region, located within the genomic island AGI-3, is composed of six genes encoding a sugar transporter and enzymes involved in fructan metabolism, and of a divergently transcribed gene encoding a transcriptional regulator, FosR, belonging to the LacI/GalR family. We demonstrated that the expression of fos genes is repressed by FosR, controlled by catabolite repression and induced in the presence of fructans. FosR binds to two operator sequences of the fos operon promoter region. This binding to DNA is inhibited in the presence of fructans, especially by 1-kestose. The fos region strongly benefits growth on cecal content and colonization of the ceca in vivo. Moreover, AGI-3 is mobile and transferable, suggesting a possible dissemination of fructan metabolism within the species E. coli
Ghiaur, Gabriel. "The role of Rho GTPases in hematopoietic stem cell biology RhoA GTPase regulates adult HSC engraftment and Rac1 GTPases is important for embryonic HSC /." Cincinnati, Ohio : University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1204374567.
Повний текст джерелаTonin, Igor. "Aplicação de ácido giberélico e superação de dormência em sementes de trigo." Universidade Federal de Pelotas, 2015. http://repositorio.ufpel.edu.br:8080/handle/prefix/3275.
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O trigo (Triticum aestivum L.) é uma cultura de destaque econômico e um dos cereais de inverno mais cultivados no sul do Brasil. Entretanto, suas sementes apresentam dormência, sendo um fator limitante em programas de melhoramento genético, quando se deseja rápida germinação pós-colheita, necessitando dessa forma, viabilizar tecnologias para potencializar a germinação. A pesquisa teve como objetivo avaliar a eficácia de doses de ácido giberélico na superação de dormência de sementes de trigo. As sementes de trigo da cultivar TBIO Toruk foram tratadas com inseticida Cropstar imidacloprid+thiodicarb 600 SC), fungicida Spectro (difenoconazol 150 SC) seguido de adição na calda do TS de GA3 nas respectivas dosagens. Os tratamentos empregados consistiram em doses de ácido giberélico, sendo: Pro-Gibb® (50 mg Kg-1 de GA3); Pro-Gibb® (100 mg Kg-1 de GA3); Pro-Gibb® (150 mg Kg-1 de GA3); Pro-Gibb® (200 mg Kg-1 de GA3); Pro-Gibb® (250 mg Kg-1 de GA3); Pro-Gibb® (300 mg Kg-1 de GA3) e testemunha (semente sem tratamento). Para avaliar a eficácia do GA3 as variáveis analisadas foram germinação, primeira contagem da germinação, velocidade de germinação, índice e coeficiente de velocidade de germinação. Os resultados obtidos permitem concluir que o ácido giberélico influenciou de forma positiva na superação da dormência de sementes de trigo, onde foi constatado maior porcentagem de germinação e vigor. A dose de ácido giberélico de 50 mg Kg-1, propiciou maior porcentagem de germinação (91 %), com incremento de 35 % na germinação de sementes de trigo quando comparado com sementes não tratadas. Por outro lado, constatou-se que doses de ácido giberélico, acima de 250 mg Kg-1, não são recomendadas para superação de dormência de sementes de trigo.
Wheat (Triticum aestivum L.) is a crop that has highlight of economic and one of the most important winter cereals grown in southern Brazil. However, the seeds present dormancy, limiting factor in breeding programs, considering that is necessary germination immediately after the harvest, requiring viable technologies to enhance germination. The research aimed to evaluate the effectiveness of gibberellic acid doses in overcoming wheat seed dormancy, It was used wheat seeds cultivar TBIO Toruk, Were treated with Cropstar insecticide (imidacloprid + thiodicarb 600 SC), Spectro fungicide (difenoconazole 150 SC) followed by the addition of the TS with GA3 in the dosis evaluated. The treatments consisted in the use of gibberellic acid doses, as follows: Pro-Gibb® (50 mg Kg-1 GA3); Pro-Gibb® (100 mg Kg-1 GA3); Pro- Gibb® (150 mg Kg-1 GA3); Pro-Gibb® (200 mg Kg-1 GA3); Pro-Gibb® (250 mg Kg-1 GA3); Pro-Gibb® (300 mg Kg-1 GA3) and Witness without treatment. To evaluate the effectiveness of GA3 it was analyzed the variables: germination, first germination count, germination rate, index and coefficient of germination speed. The obtained results allow us to conclude that gibberellic acid positively influenced the physiological quality of wheat seeds, where was found greater percentage of germination and vigor. A gibberellic acid dose of 50 mg Kg-1, show greater germination percentage (91 %), an increase of 35 % in wheat seeds germination when compared to untreated seeds. Gibberellic acid doses above 250 mg Kg-1, are not recommended for overcoming wheat seed dormancy.
Bruneau, Alix. "Régulation de l'expression membranaire du transporteur de phospholipides biliaires ABCB4 : effet de mutations Functional Defect of Variants in the Adenosine Triphosphate–Binding Sites of ABCB4 and Their Rescue by the Cystic Fibrosis Transmembrane Conductance Regulator Potentiator, Ivacaftor (VX-770) Structural analogues of roscovitine rescue the intracellular traffic and the function of ER-retained ABCB4 variants in cell models." Thesis, Sorbonne université, 2019. http://www.theses.fr/2019SORUS048.
Повний текст джерелаABCB4 is exclusively expressed at the canalicular membrane of hepatocytes where its function is to translocate phosphatidylcholine (PC) into bile. Variations in ABCB4 gene sequence are associated with several chronic and progressive liver diseases. The most severe is PFIC3 which develops early in childhood and most often requires liver transplantation. Less severe diseases are the intrahepatic cholestasis of pregnancy and the low phospholipid- associated cholelithiasis syndrome which occur in young adults. Up to now, about 500 disease-causing ABCB4 variants have been reported. A challenge is to find pharmacological treatments for the severe forms of the diseases. We have studied the effect of five disease-causing variations that reside in the highly conserved motifs of ABC transporters, involved in ATP binding. Using three-dimension structural modeling and in vitro studies, we showed that the five mutants were normally processed and targeted to the plasma membrane, whereas their PC secretion activity was dramatically decreased. PC secretion activity of the mutants was rescued by the clinically approved CFTR potentiator ivacaftor (VX-770). These results pave the way for personalized therapy in ABCB4-related diseases.The second part of my project was aimed at investigating the potential role of two ABCB4 partners, the kinase MRCKalpha and its effector the myosin light chain II (MLCII) in the expression and function of ABCB4. We found that downregulation of both partners didn’t affect the canalicular localization of ABCB4 but led to a reduction of its endocytosis. Our results open new insights into the mechanisms underlying the regulation of ABCB4 expression and function
Al-Amri, Jehad. "An analysis of the influence of cultural backgrounds of individuals upon their perspective towards privacy within internet activities." Thesis, De Montfort University, 2012. http://hdl.handle.net/2086/9028.
Повний текст джерелаGlaser, Cornelia. "Förderung der Schreibkompetenz bei Grundschülern : Effekte einer integrierten Vermittlung kognitiver Schreibstrategien und selbstregulatorischer Fertigkeiten." Phd thesis, Universität Potsdam, 2004. http://opus.kobv.de/ubp/volltexte/2005/217/.
Повний текст джерелаDie Wirksamkeit des Trainings wurde in drei Studien untersucht: 1. Eine Pilotstudie diente der Überprüfung der prinzipiellen Eignung von SAT zur Förderung von Schreibleistungen bei Grundschülern der 5. Klassen (N = 42) und der Optimierung seiner Teilkomponenten und Vorgehensweisen. 2. In der Hauptuntersuchung wurden die Effektivität und Nachhaltigkeit des SAT-Programms bei Schülern der 4. Klasse (N = 154) im Vergleich zu zwei Bedingungen getestet: (a) der isolierten Einübung von Schreibstrategien (Aufsatztraining) und (b) konventionellem Aufsatzunterricht (Unterrichtskontrollgruppe). 3. In einer weiteren Studie wurde die Wirksamkeit des Trainings speziell bei Schülern mit ungünstigen Lernvoraussetzungen überprüft; die Studie diente zudem der Illustration des dabei gewählten Vorgehens am Einzelfall (N = 6).
Die ermittelten Befunde sprechen übereinstimmend dafür, dass die Kombination aus strategischem plus selbstregulatorischem Training (SAT) die stärksten und nachhaltigsten Effekte auf die Schreibleistung erzielt. Der Trainingseffekt generalisiert zudem auf die Erinnerungsleistung bei der freien Wiedergabe einer Kurzgeschichte. Schüler mit schwachen Aufsatzleistungen und ungünstigen Lernvoraussetzungen profitieren von dem SAT-Programm in besonderem Maße.
In der Diskussion werden Aufgaben für die zukünftige Forschung erörtert. Forschungsbedarf besteht u.a. hinsichtlich (a) einer stärkeren Verknüpfung von Schreibtrainings mit der kognitionspsychologischen Forschung; (b) der Dekomposition und gezielten Überprüfung der einzelnen Trainingskomponenten; (c) der Ausweitung des SAT-Programms auf andere Textgenre; (d) der Integration verfeinerter Revisionsstrategien in das Förderprogramm; und (e) dessen Implementierung in den Regelunterricht.
Extending on Harris and Graham′s (1996) Self-Regulated-Strategy-Development-Model, I designed an curriculum-integrated intervention program (SAT) to promote the composition skills of elementary school-age students. SAT combines the instruction of task strategies required to write good narratives with the explicit instruction of self-regulation procedures (goal setting, strategic planning, self-evaluation, self-correction).
Three studies examined the effectiveness of the training: 1. A pilot study investigated the viability of the SAT-program among 5th graders (N = 42) and served to refine its components and procedures. 2. In a sample of 4th graders (N = 154), the main study tested the strength and stability of the SAT effects in relation to two comparison groups: (a) Students who were taught the same set of task strategies but received no instruction in self-regulation procedures (strategy-only condition); (b) students who received conventional classroom teaching in composing (control condition). (3.) A third study served to examine the effectiveness of the SAT-program in a group of low achieving 5th graders and to illustrate its instructional steps in a number of single cases (N = 6).
Results obtained from these studies converge in showing that a writing program that conjointly addresses both task strategies and self-regulation procedures (SAT) is most effective in producing strong and lasting effects on elementary school students′ composing skills and generalization performance. Among all students, low achievers were most likely to benefit from the SAT-program.
The discussion highlights a number of issues for future research on writing. Specifically, it is argued that there is a need to (a) further explore the cognitive and meta-cognitive processes underlying good writing, (b) examine the effectiveness of specific training components incorporated in the present version of SAT, (c) crossvalidate the reported SAT effects with respect to various writing genre, (d) incorporate more elaborated revision strategies into the training program, and (e) implement components and procedures specified in SAT into conventional classroom teaching.
Lingeswaran, Abarna. "Rôle clé du transporteur PptAB dans le quorum sensing chez Streptococcus thermophilus." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASB013.
Повний текст джерелаIn Streptococcus thermophilus, the life cycle of signaling peptides called pheromones contributing to communication mechanisms named quorum sensing (QS) is divided into four steps. The synthesis of pheromones is followed by their maturation by the protease Eep and their secretion. At last, their re-internalisation by the oligopeptide transporter Ami is essential for their intracellular detection by transcriptional regulators called Rgg-like controlling the expression of target genes. The aim of my thesis was to valid the role of the transporter PptAB in the secretion of pheromones before identifying genes whose expression is dependent of this transporter in S. thermophilus.First, we confirmed the role of the transporter PptAB in the activation of three QS systems involving Rgg-like regulators. For that we used transcriptional fusions between a reporter gene coding the luciferase and the promoter of three target genes of these systems. We then showed that the transporter PptAB exports more likely only the mature form of pheromones by LC-MS/MS. Finally, we discovered that the expression of a set of genes located downstream of genes coding Rgg-like regulators is dependant of the transporter PptAB and also of the transporter Ami and the protease Eep by a global transcriptomic approach. Thereby, the transporters PptAB and Ami and the protease Eep regulate these targets by a same mechanism.Our work brought light on cross-talks between these systems which need to be deciphered
Arora, Amandeep Singh [Verfasser], Matthias [Akademischer Betreuer] Bahr, Mikael [Akademischer Betreuer] Simons, and Uwe-Karsten [Akademischer Betreuer] Hanisch. "Functional proteome analysis of age associated PrPC knockout mice liver along with regulatory response of cytoskeleton associated tau protein and fatty liver disease. / Amandeep Singh Arora. Betreuer: Matthias Bahr. Gutachter: Mikael Simons ; Uwe-Karsten Hanisch." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2015. http://d-nb.info/1079718095/34.
Повний текст джерелаŠoupal, Ondřej. "Programování mikrokontrolérů c2000 v programu MATLAB/Simulink." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2020. http://www.nusl.cz/ntk/nusl-413221.
Повний текст джерелаCARAMANICO, LEILA. "STUDY OF GRAPEVINE ROOTSTOCK RESPONSE TO WATER STRESS." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/707586.
Повний текст джерелаGUPTA, NIDHI. "AUTOMATIC GENERATION CONTROL OF INTERCONNECTED MULTI AREA POWER SYSTEM." Thesis, 2022. http://dspace.dtu.ac.in:8080/jspui/handle/repository/19386.
Повний текст джерелаPham, Thi Ngoc Thang. "Ripening behaviour of capsicum (Capsicum annuum L.) fruit." 2007. http://hdl.handle.net/2440/40395.
Повний текст джерелаhttp://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1294648
Thesis(Ph.D.)-- School of Agriculture, Food and Wine, 2007
Pham, Thi Ngoc Thang. "Ripening behaviour of capsicum (Capsicum annuum L.) fruit." Thesis, 2007. http://hdl.handle.net/2440/40395.
Повний текст джерелаThesis(Ph.D.)-- School of Agriculture, Food and Wine, 2007
Finezzo, Laura. "FLAVONOID BIOSYNTHESIS IN GRAPEVINE: THE ROLE OF NOVEL MYB TRANSCRIPTIONAL REGULATORS." Doctoral thesis, 2014. http://hdl.handle.net/11562/666358.
Повний текст джерелаThe R2R3 MYB is the largest group of transcriptional regulators in plant. They are involved in different process such as the control of the production of trichomes and root hairs. A part of these MYBs participates to a transcriptional regulatory complex in which includes also a WD40-repeat (WDR) protein and a basic-Helix-Loop-Helix (bHLH) protein. The MYB factor play an important role because it is the major responsible for the specificity of action of the complex. The MYB-bHLH-WDR regulatory complex may act as a transcriptional activator or as a transcriptional repressor: activation function in which MYB activators participate to the complex and activate the transcription of genes by direct binding to their promoters and repression function in which MYB repressors, through different ways, repress the transcription. In Vitis vinifera the MYB-bHLH-WDR complex has been partially studied in relation to the flavonoid pathway, and some MYB factors have been shown to play a role in driving the complex in the activation of specific branches of the pathway. For example, VvMYBA1 and VvMYBA2 are factors responsible for the production of anthocyanin pigments that color the berry skin (Walker et al., 2007), while VvMYBPA1 and VvMYBPA2 are responsible for the production of proanthocyanidins. Thanks to the recently released grape genome we could identify several genes highly homologous to known anthocyanin pathway regulators of other species. In a phylogenetic tree of Vitis vinifera MYB factors there is a clade where VvMYBA1 and VvMYBA2 cluster together with few other MYBs and to date only the MYBA1 and MYBA2 have been characterized. In this clade, three highly homologous genes residing on the same chromosome (Chromosome 2) were named VvMYBA5, VvMYBA6 and VvMYBA7, and chosen for further characterization. Due to the high homology with the characterized VvMYBA1 and VvMYBA2, we hypothesized that they could regulate the anthocyanin synthesis in different organs/stages respect to VvMYBA1. In the same way, we found another clade, called “repressors clade” in witch the proteins are characterized by the presence of the repression motif. The information regarding the MYB repressors in Vitis vinifera is scarce and only one MYB repressor has been identified until now. So another part of my PhD project is the functional characterization of different MYB proteins present in this clade utilizing different approaches as, the heterologous expression in Petunia hybirda, the ectopic overexpression in grapevine hairy roots and the Yeast two hybrid analysis. In details, I choose five different MYB repressors, named VvMYB4a, VvMYB4b, VvMYBC2-L1, VvMYBC2-L2 and VvMYBC2-L3, based on the homology with other repressors belonging to a different species and on particular amino acidic signatures. Moreover, I initiated a study of some MYB proteins belonging to the R2R3 MYB repressor clade, almost completely uncharacterized in grapevine. Finally, I tentatively characterized, a grapevine MYB with a single R3 repeat.
Nodari, Alice. "Interferon Regulatory Factor 7 (IRF7) inhibition reverts age induced transcriptional and metabolic derangement." Doctoral thesis, 2020. http://hdl.handle.net/11562/1018390.
Повний текст джерелаBirgi, Elif Nazli. "AML/CFT regulations of EU in the age of virtual currency." Master's thesis, 2018. http://hdl.handle.net/1822/60949.
Повний текст джерелаGlobal consideration on money laundering has its origins in narco-trafficking of 1980s which raised public awareness and took international regulatory body’s attention. Throughout time, due to the socio-economic and political context, legislations on money laundering were transformed in order to introduce an efficient response to new issues. As a need in the aftermath of 9/11, counter-financing of terrorism (CFT) was included in the scope of anti-money laundering (AML) legislations, due to the intertwined nature of these two criminal matters. A new challenge to the AML/CFT legislations was introduced by the technological developments and the emergence of virtual currency. Appearing as an alternative, fast, easy and cheap non-cash payment method, its relation with criminal activities, widespread usage and unregulated operations raised concerns. When traditional approaches to the fight against money laundering and financing of terrorism were circumvented by pseudo-anonymous and decentralized nature of new transaction methods, existing legislations were forced to be transformed once more. European Union, taking its powers for regulating criminal matters from the Treaty of the Functioning of European Union (TFEU), proposed an amendment to the 4th AML, with the purpose of reducing anonymity of virtual currency. Not being accepted yet, its ability to produce an adequate respond to challenges, due to the special nature of virtual currency, is questionable. This thesis analyse European Union’s current Anti-Money Laundering legislation and its responsiveness to the characteristics of virtual currency that are attributable to the risks, with particular attention to crypto-currency, through a critical perspective. It aimed to raise awareness of the subject matter and contribute to the future of AML/CFT reuglations of the EU.
A preocupação internacional com o branqueamento de capitais está ligada ao narcotráfico da década de oitenta. Ao longo do tempo e devido ao contexto sócio económico e político, a legislação relacionada com o branqueamento de capitais foi sendo adaptada, permitindo introduzir uma resposta mais eficiente aos novos desafios. Isto foi particularmente visível na sequência dos ataques de 11 de setembro, momento a partir do qual a prevenção do financiamento do terrorismo passou a estar incluída no domínio do branqueamento de capitais, atendendo à ligação próxima entre estes dois fenómenos. Um novo desafio à legislação sobre branqueamento de capitais surgiu como desenvolvimento tecnológico, nomeadamente com o aparecimento de cripto-moedas. As moedas virtuais surgiram como uma alternativa rápida, fácil e pouco dispendiosa, para realizar pagamentos. Porém, a sua associação a atividades criminosas, uso generalizado e ausência de regulamentação própria conduziram a fortes preocupações por parte das entidades reguladoras. As abordagens tradicionais de combate à lavagem de dinheiro e financiamento do terrorismo tornaram-se obsoletas perante a natureza descentralizada e pseudoanónima destes novos métodos de transações, demandando uma reforma célere da legislação existente. A União Europeia, utilizando o Tratado sobre o Funcionamento da União Europeia como forma de fundamentar os seus poderes, propôs uma alteração à diretiva 4.ª AML, com o objetivo de reduzir o anonimato das cripto-moedas. Não tendo sido ainda aprovada, a capacidade desta alteração produzir a resposta adequada aos desafios apresentados pela natureza especial das moedas virtuais é, no mínimo, questionável. O trabalho aqui apresentado analisa a atual legislação europeia contra o branqueamento de capitais e a sua capacidade de responder às características das moedas virtuais, às quais se atribui um elevado risco. Tem também como objetivo salientar questões relativas a esta temática e despertar maior interesse, assim como contribuir para o futuro da regulamentação AML/CFT da União Europeia.
Hardman, J. A., Desmond J. Tobin, I. S. Haslam, N. P. Farjo, B. K. Farjo, Y. Al-Nuaimi, B. Grimaldi, and R. Paus. "The Peripheral Clock Regulates Human Pigmentation." 2014. http://hdl.handle.net/10454/7429.
Повний текст джерелаAlthough the regulation of pigmentation is well characterized, it remains unclear whether cell-autonomous controls regulate the cyclic on–off switching of pigmentation in the hair follicle (HF). As human HFs and epidermal melanocytes express clock genes and proteins, and given that core clock genes (PER1, BMAL1) modulate human HF cycling, we investigated whether peripheral clock activity influences human HF pigmentation. We found that silencing BMAL1 or PER1 in human HFs increased HF melanin content. Furthermore, tyrosinase expression and activity, as well as TYRP1 and TYRP2 mRNA levels, gp100 protein expression, melanocyte dendricity, and the number gp100+ HF melanocytes, were all significantly increased in BMAL1 and/or PER1-silenced HFs. BMAL1 or PER1 silencing also increased epidermal melanin content, gp100 protein expression, and tyrosinase activity in human skin. These effects reflect direct modulation of melanocytes, as BMAL1 and/or PER1 silencing in isolated melanocytes increased tyrosinase activity and TYRP1/2 expression. Mechanistically, BMAL1 knockdown reduces PER1 transcription, and PER1 silencing induces phosphorylation of the master regulator of melanogenesis, microphthalmia-associated transcription factor, thus stimulating human melanogenesis and melanocyte activity in situ and in vitro. Therefore, the molecular clock operates as a cell-autonomous modulator of human pigmentation and may be targeted for future therapeutic strategies.