Статті в журналах: "Advanced bioink"

1

Gu, Yawei, Benjamin Schwarz, Aurelien Forget, Andrea Barbero, Ivan Martin, and V. Prasad Shastri. "Advanced Bioink for 3D Bioprinting of Complex Free-Standing Structures with High Stiffness." Bioengineering 7, no. 4 (November 7, 2020): 141. http://dx.doi.org/10.3390/bioengineering7040141.

Повний текст джерела

Анотація:

One of the challenges in 3D-bioprinting is the realization of complex, volumetrically defined structures, that are also anatomically accurate and relevant. Towards this end, in this study we report the development and validation of a carboxylated agarose (CA)-based bioink that is amenable to 3D printing of free-standing structures with high stiffness at physiological temperature using microextrusion printing without the need for a fugitive phase or post-processing or support material (FRESH). By blending CA with negligible amounts of native agarose (NA) a bioink formulation (CANA) which is suitable for printing with nozzles of varying internal diameters under ideal pneumatic pressure was developed. The ability of the CANA ink to exhibit reproducible sol-gel transition at physiological temperature of 37 °C was established through rigorous characterization of the thermal behavior, and rheological properties. Using a customized bioprinter equipped with temperature-controlled nozzle and print bed, high-aspect ratio objects possessing anatomically-relevant curvature and architecture have been printed with high print reproducibility and dimension fidelity. Objects printed with CANA bioink were found to be structurally stable over a wide temperature range of 4 °C to 37 °C, and exhibited robust layer-to-layer bonding and integration, with evenly stratified structures, and a porous interior that is conducive to fluid transport. This exceptional layer-to-layer fusion (bonding) afforded by the CANA bioink during the print obviated the need for post-processing to stabilize printed structures. As a result, this novel CANA bioink is capable of yielding large (5–10 mm tall) free-standing objects ranging from simple tall cylinders, hemispheres, bifurcated ‘Y’-shaped and ‘S’-shaped hollow tubes, and cylinders with compartments without the need for support and/or a fugitive phase. Studies with human nasal chondrocytes showed that the CANA bioink is amenable to the incorporation of high density of cells (30 million/mL) without impact on printability. Furthermore, printed cells showed high viability and underwent mitosis which is necessary for promoting remodeling processes. The ability to print complex structures with high cell densities, combined with excellent cell and tissue biocompatibility of CA bodes well for the exploitation of CANA bioinks as a versatile 3D-bioprinting platform for the clinical translation of regenerative paradigms.

Стилі APA, Harvard, Vancouver, ISO та ін.

2

Gao, Qiqi, Byoung-Soo Kim, and Ge Gao. "Advanced Strategies for 3D Bioprinting of Tissue and Organ Analogs Using Alginate Hydrogel Bioinks." Marine Drugs 19, no. 12 (December 15, 2021): 708. http://dx.doi.org/10.3390/md19120708.

Повний текст джерела

Анотація:

Alginate is a natural polysaccharide that typically originates from various species of algae. Due to its low cost, good biocompatibility, and rapid ionic gelation, the alginate hydrogel has become a good option of bioink source for 3D bioprinting. However, the lack of cell adhesive moieties, erratic biodegradability, and poor printability are the critical limitations of alginate hydrogel bioink. This review discusses the pivotal properties of alginate hydrogel as a bioink for 3D bioprinting technologies. Afterward, a variety of advanced material formulations and biofabrication strategies that have recently been developed to overcome the drawbacks of alginate hydrogel bioink will be focused on. In addition, the applications of these advanced solutions for 3D bioprinting of tissue/organ mimicries such as regenerative implants and in vitro tissue models using alginate-based bioink will be systematically summarized.

Стилі APA, Harvard, Vancouver, ISO та ін.

3

Khati, Vamakshi, Harisha Ramachandraiah, Falguni Pati, Helene A. Svahn, Giulia Gaudenzi, and Aman Russom. "3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological Temperatures." Biosensors 12, no. 7 (July 13, 2022): 521. http://dx.doi.org/10.3390/bios12070521.

Повний текст джерела

Анотація:

Bioprinting is an acclaimed technique that allows the scaling of 3D architectures in an organized pattern but suffers from a scarcity of appropriate bioinks. Decellularized extracellular matrix (dECM) from xenogeneic species has garnered support as a biomaterial to promote tissue-specific regeneration and repair. The prospect of developing dECM-based 3D artificial tissue is impeded by its inherent low mechanical properties. In recent years, 3D bioprinting of dECM-based bioinks modified with additional scaffolds has advanced the development of load-bearing constructs. However, previous attempts using dECM were limited to low-temperature bioprinting, which is not favorable for a longer print duration with cells. Here, we report the development of a multi-material decellularized liver matrix (dLM) bioink reinforced with gelatin and polyethylene glycol to improve rheology, extrudability, and mechanical stability. This shear-thinning bioink facilitated extrusion-based bioprinting at 37 °C with HepG2 cells into a 3D grid structure with a further enhancement for long-term applications by enzymatic crosslinking with mushroom tyrosinase. The heavily crosslinked structure showed a 16-fold increase in viscosity (2.73 Pa s−1) and a 32-fold increase in storage modulus from the non-crosslinked dLM while retaining high cell viability (85–93%) and liver-specific functions. Our results show that the cytocompatible crosslinking of dLM bioink at physiological temperatures has promising applications for extended 3D-printing procedures.

Стилі APA, Harvard, Vancouver, ISO та ін.

4

Salg, Gabriel Alexander, Andreas Blaeser, Jamina Sofie Gerhardus, Thilo Hackert, and Hannes Goetz Kenngott. "Vascularization in Bioartificial Parenchymal Tissue: Bioink and Bioprinting Strategies." International Journal of Molecular Sciences 23, no. 15 (August 2, 2022): 8589. http://dx.doi.org/10.3390/ijms23158589.

Повний текст джерела

Анотація:

Among advanced therapy medicinal products, tissue-engineered products have the potential to address the current critical shortage of donor organs and provide future alternative options in organ replacement therapy. The clinically available tissue-engineered products comprise bradytrophic tissue such as skin, cornea, and cartilage. A sufficient macro- and microvascular network to support the viability and function of effector cells has been identified as one of the main challenges in developing bioartificial parenchymal tissue. Three-dimensional bioprinting is an emerging technology that might overcome this challenge by precise spatial bioink deposition for the generation of a predefined architecture. Bioinks are printing substrates that may contain cells, matrix compounds, and signaling molecules within support materials such as hydrogels. Bioinks can provide cues to promote vascularization, including proangiogenic signaling molecules and cocultured cells. Both of these strategies are reported to enhance vascularization. We review pre-, intra-, and postprinting strategies such as bioink composition, bioprinting platforms, and material deposition strategies for building vascularized tissue. In addition, bioconvergence approaches such as computer simulation and artificial intelligence can support current experimental designs. Imaging-derived vascular trees can serve as blueprints. While acknowledging that a lack of structured evidence inhibits further meta-analysis, this review discusses an end-to-end process for the fabrication of vascularized, parenchymal tissue.

Стилі APA, Harvard, Vancouver, ISO та ін.

5

Bednarzig, Vera, Emine Karakaya, Aldo Leal Egaña, Jörg Teßmar, Aldo R. Boccaccini, and Rainer Detsch. "Advanced ADA-GEL bioink for bioprinted artificial cancer models." Bioprinting 23 (August 2021): e00145. http://dx.doi.org/10.1016/j.bprint.2021.e00145.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

6

Lee, Kangseok, and Chaenyung Cha. "Advanced Polymer-Based Bioink Technology for Printing Soft Biomaterials." Macromolecular Research 28, no. 8 (July 2020): 689–702. http://dx.doi.org/10.1007/s13233-020-8134-9.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

7

Hu, Chen, Taufiq Ahmad, Malik Salman Haider, Lukas Hahn, Philipp Stahlhut, Jürgen Groll, and Robert Luxenhofer. "A thermogelling organic-inorganic hybrid hydrogel with excellent printability, shape fidelity and cytocompatibility for 3D bioprinting." Biofabrication 14, no. 2 (January 24, 2022): 025005. http://dx.doi.org/10.1088/1758-5090/ac40ee.

Повний текст джерела

Анотація:

Abstract Alginates are the most commonly used bioink in biofabrication, but their rheological profiles make it very challenging to perform real 3D printing. In this study, an advanced hybrid hydrogel ink was developed, a mixture of thermogelling diblock copolymer, alginate and clay i.e. Laponite XLG. The reversible thermogelling and shear thinning properties of the diblock copolymer in the ink system improves handling and 3D printability significantly. Various three-dimensional constructs, including suspended filaments, were printed successfully with high shape fidelity and excellent stackability. Subsequent ionic crosslinking of alginate fixates the printed scaffolds, while the diblock copolymer is washed out of the structure, acting as a fugitive material/porogen on the (macro)molecular level. Finally, cell-laden printing and culture over 21 d demonstrated good cytocompatibility and feasibility of the novel hybrid hydrogels for 3D bioprinting. We believe that the developed approach could be interesting for a wide range of bioprinting applications including tissue engineering and drug screening, potentially enabling also other biological bioinks such as collagen, hyaluronic acid, decellularized extracellular matrices or cellulose based bioinks.

Стилі APA, Harvard, Vancouver, ISO та ін.

8

Kostenko, Anastassia, Che J. Connon, and Stephen Swioklo. "Storable Cell-Laden Alginate Based Bioinks for 3D Biofabrication." Bioengineering 10, no. 1 (December 23, 2022): 23. http://dx.doi.org/10.3390/bioengineering10010023.

Повний текст джерела

Анотація:

Over the last decade, progress in three dimensional (3D) bioprinting has advanced considerably. The ability to fabricate complex 3D structures containing live cells for drug discovery and tissue engineering has huge potential. To realise successful clinical translation, biologistics need to be considered. Refinements in the storage and transportation process from sites of manufacture to the clinic will enhance the success of future clinical translation. One of the most important components for successful 3D printing is the ‘bioink’, the cell-laden biomaterial used to create the printed structure. Hydrogels are favoured bioinks used in extrusion-based bioprinting. Alginate, a natural biopolymer, has been widely used due to its biocompatibility, tunable properties, rapid gelation, low cost, and easy modification to direct cell behaviour. Alginate has previously demonstrated the ability to preserve cell viability and function during controlled room temperature (CRT) storage and shipment. The novelty of this research lies in the development of a simple and cost-effective hermetic system whereby alginate-encapsulated cells can be stored at CRT before being reformulated into an extrudable bioink for on-demand 3D bioprinting of cell-laden constructs. To our knowledge the use of the same biomaterial (alginate) for storage and on-demand 3D bio-printing of cells has not been previously investigated. A straightforward four-step process was used where crosslinked alginate containing human adipose-derived stem cells was stored at CRT before degelation and subsequent mixing with a second alginate. The printability of the resulting bioink, using an extrusion-based bioprinter, was found to be dependent upon the concentration of the second alginate, with 4 and 5% (w/v) being optimal. Following storage at 15 °C for one week, alginate-encapsulated human adipose-derived stem cells exhibited a high viable cell recovery of 88 ± 18%. Stored cells subsequently printed within 3D lattice constructs, exhibited excellent post-print viability and even distribution. This represents a simple, adaptable method by which room temperature storage and biofabrication can be integrated for on-demand bioprinting.

Стилі APA, Harvard, Vancouver, ISO та ін.

9

Rocca, Marco, Alessio Fragasso, Wanjun Liu, Marcel A. Heinrich, and Yu Shrike Zhang. "Embedded Multimaterial Extrusion Bioprinting." SLAS TECHNOLOGY: Translating Life Sciences Innovation 23, no. 2 (November 13, 2017): 154–63. http://dx.doi.org/10.1177/2472630317742071.

Повний текст джерела

Анотація:

Embedded extrusion bioprinting allows for the generation of complex structures that otherwise cannot be achieved with conventional layer-by-layer deposition from the bottom, by overcoming the limits imposed by gravitational force. By taking advantage of a hydrogel bath, serving as a sacrificial printing environment, it is feasible to extrude a bioink in freeform until the entire structure is deposited and crosslinked. The bioprinted structure can be subsequently released from the supporting hydrogel and used for further applications. Combining this advanced three-dimensional (3D) bioprinting technique with a multimaterial extrusion printhead setup enables the fabrication of complex volumetric structures built from multiple bioinks. The work described in this paper focuses on the optimization of the experimental setup and proposes a workflow to automate the bioprinting process, resulting in a fast and efficient conversion of a virtual 3D model into a physical, extruded structure in freeform using the multimaterial embedded bioprinting system. It is anticipated that further development of this technology will likely lead to widespread applications in areas such as tissue engineering, pharmaceutical testing, and organs-on-chips.

Стилі APA, Harvard, Vancouver, ISO та ін.

10

Zhang, Lei, Hai Tang, Zijie Xiahou, Jiahui Zhang, Yunlang She, Kunxi Zhang, Xuefei Hu, Jingbo Yin, and Chang Chen. "Solid multifunctional granular bioink for constructing chondroid basing on stem cell spheroids and chondrocytes." Biofabrication 14, no. 3 (April 13, 2022): 035003. http://dx.doi.org/10.1088/1758-5090/ac63ee.

Повний текст джерела

Анотація:

Abstract Stem cell spheroids are advanced building blocks to produce chondroid. However, the multi-step operations including spheroids preparation, collection and transfer, the following 3D printing and shaping limit their application in 3D printing. The present study fabricates an ‘ALL-IN-ONE’ bioink based on granular hydrogel to not only produce adipose derived stem cell (ASC) spheroids, but also realize the further combination of chondrocytes and the subsequent 3D printing. Microgels (6–10 μm) grafted with β-cyclodextrin (β-CD) (MGβ-CD) were assembled and crosslinked by in-situ polymerized poly (N-isopropylacrylamide) (PNIPAm) to form bulk granular hydrogel. The host-guest action between β-CD of microgels and PNIPAm endows the hydrogel with stable, shear-thinning and self-healing properties. After creating caves, ASCs aggregate spontaneously to form numerous spheroids with diameter of 100–200 μm inside the hydrogel. The thermosensitive porous granular hydrogel exhibits volume change under different temperature, realizing further adsorbing chondrocytes. Then, the granular hydrogel carrying ASC spheroids and chondrocytes is extruded by 3D printer at room temperature to form a tube, which can shrink at cell culture temperature to enhance the resolution. The subsequent ASC spheroids/chondrocytes co-culture forms cartilage-like tissue at 21 d in vitro, which further matures subcutaneously in vivo, indicating the application potential of the fully synthetic granular hydrogel ink toward organoid culture.

Стилі APA, Harvard, Vancouver, ISO та ін.

11

Kunze Küllmer, M., C. Hidalgo, A. Zaupa, G. Zavala, J. Acevedo, M. Khoury, S. Viafara, C. F. Terraza, N. Byres, and P. Abarzua. "Physical and immuno-engineering of an advanced bioink based on a cold-adapted biomaterial for multi-material high-resolution 3D bioprinting." Cytotherapy 23, no. 5 (May 2021): S144. http://dx.doi.org/10.1016/s1465324921005120.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

12

Masri, Syafira, Mazlan Zawani, Izzat Zulkiflee, Atiqah Salleh, Nur Izzah Md Fadilah, Manira Maarof, Adzim Poh Yuen Wen, et al. "Cellular Interaction of Human Skin Cells towards Natural Bioink via 3D-Bioprinting Technologies for Chronic Wound: A Comprehensive Review." International Journal of Molecular Sciences 23, no. 1 (January 1, 2022): 476. http://dx.doi.org/10.3390/ijms23010476.

Повний текст джерела

Анотація:

Skin substitutes can provide a temporary or permanent treatment option for chronic wounds. The selection of skin substitutes depends on several factors, including the type of wound and its severity. Full-thickness skin grafts (SGs) require a well-vascularised bed and sometimes will lead to contraction and scarring formation. Besides, donor sites for full-thickness skin grafts are very limited if the wound area is big, and it has been proven to have the lowest survival rate compared to thick- and thin-split thickness. Tissue engineering technology has introduced new advanced strategies since the last decades to fabricate the composite scaffold via the 3D-bioprinting approach as a tissue replacement strategy. Considering the current global donor shortage for autologous split-thickness skin graft (ASSG), skin 3D-bioprinting has emerged as a potential alternative to replace the ASSG treatment. The three-dimensional (3D)-bioprinting technique yields scaffold fabrication with the combination of biomaterials and cells to form bioinks. Thus, the essential key factor for success in 3D-bioprinting is selecting and developing suitable bioinks to maintain the mechanisms of cellular activity. This crucial stage is vital to mimic the native extracellular matrix (ECM) for the sustainability of cell viability before tissue regeneration. This comprehensive review outlined the application of the 3D-bioprinting technique to develop skin tissue regeneration. The cell viability of human skin cells, dermal fibroblasts (DFs), and keratinocytes (KCs) during in vitro testing has been further discussed prior to in vivo application. It is essential to ensure the printed tissue/organ constantly allows cellular activities, including cell proliferation rate and migration capacity. Therefore, 3D-bioprinting plays a vital role in developing a complex skin tissue structure for tissue replacement approach in future precision medicine.

Стилі APA, Harvard, Vancouver, ISO та ін.

13

Ngan, Catherine G. Y., Anita Quigley, Richard J. Williams, Cathal D. O’Connell, Romane Blanchard, Mitchell Boyd-Moss, Tim D. Aumann, et al. "Matured Myofibers in Bioprinted Constructs with In Vivo Vascularization and Innervation." Gels 7, no. 4 (October 15, 2021): 171. http://dx.doi.org/10.3390/gels7040171.

Повний текст джерела

Анотація:

For decades, the study of tissue-engineered skeletal muscle has been driven by a clinical need to treat neuromuscular diseases and volumetric muscle loss. The in vitro fabrication of muscle offers the opportunity to test drug-and cell-based therapies, to study disease processes, and to perhaps, one day, serve as a muscle graft for reconstructive surgery. This study developed a biofabrication technique to engineer muscle for research and clinical applications. A bioprinting protocol was established to deliver primary mouse myoblasts in a gelatin methacryloyl (GelMA) bioink, which was implanted in an in vivo chamber in a nude rat model. For the first time, this work demonstrated the phenomenon of myoblast migration through the bioprinted GelMA scaffold with cells spontaneously forming fibers on the surface of the material. This enabled advanced maturation and facilitated the connection between incoming vessels and nerve axons in vivo without the hindrance of a scaffold material. Immunohistochemistry revealed the hallmarks of tissue maturity with sarcomeric striations and peripherally placed nuclei in the organized bundles of muscle fibers. Such engineered muscle autografts could, with further structural development, eventually be used for surgical reconstructive purposes while the methodology presented here specifically has wide applications for in vitro and in vivo neuromuscular function and disease modelling.

Стилі APA, Harvard, Vancouver, ISO та ін.

14

Kim, Byoung Soo, Yang Woo Kwon, Jeong-Sik Kong, Gyu Tae Park, Ge Gao, Wonil Han, Moon-Bum Kim, Hyungseok Lee, Jae Ho Kim, and Dong-Woo Cho. "3D cell printing of in vitro stabilized skin model and in vivo pre-vascularized skin patch using tissue-specific extracellular matrix bioink: A step towards advanced skin tissue engineering." Biomaterials 168 (June 2018): 38–53. http://dx.doi.org/10.1016/j.biomaterials.2018.03.040.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

15

Loukelis, Konstantinos, Zina A. Helal, Antonios G. Mikos, and Maria Chatzinikolaidou. "Nanocomposite Bioprinting for Tissue Engineering Applications." Gels 9, no. 2 (January 24, 2023): 103. http://dx.doi.org/10.3390/gels9020103.

Повний текст джерела

Анотація:

Bioprinting aims to provide new avenues for regenerating damaged human tissues through the controlled printing of live cells and biocompatible materials that can function therapeutically. Polymeric hydrogels are commonly investigated ink materials for 3D and 4D bioprinting applications, as they can contain intrinsic properties relative to those of the native tissue extracellular matrix and can be printed to produce scaffolds of hierarchical organization. The incorporation of nanoscale material additives, such as nanoparticles, to the bulk of inks, has allowed for significant tunability of the mechanical, biological, structural, and physicochemical material properties during and after printing. The modulatory and biological effects of nanoparticles as bioink additives can derive from their shape, size, surface chemistry, concentration, and/or material source, making many configurations of nanoparticle additives of high interest to be thoroughly investigated for the improved design of bioactive tissue engineering constructs. This paper aims to review the incorporation of nanoparticles, as well as other nanoscale additive materials, to printable bioinks for tissue engineering applications, specifically bone, cartilage, dental, and cardiovascular tissues. An overview of the various bioinks and their classifications will be discussed with emphasis on cellular and mechanical material interactions, as well the various bioink formulation methodologies for 3D and 4D bioprinting techniques. The current advances and limitations within the field will be highlighted.

Стилі APA, Harvard, Vancouver, ISO та ін.

16

Arnold, Anne M., Zachary C. Kennedy, and Janine R. Hutchison. "A simple, cost-effective colorimetric assay for aluminum ions via complexation with the flavonoid rutin." PeerJ Analytical Chemistry 4 (October 27, 2022): e19. http://dx.doi.org/10.7717/peerj-achem.19.

Повний текст джерела

Анотація:

Aluminum has been linked to deleterious health effects with high concentration, chronic exposure, creating a need for innovative detection techniques. Colorimetric assays are an ideal approach since they are simple, cost-effective, and field adaptable. Yet, commercially available colorimetric assays for aluminum are limited since it forms few colored chelation complexes. Flavonoids, a class of polyphenolic compounds, are one of the few examples that create colored aluminum complexes. Aluminum ions (Al3+) are the main constituent in colorimetric assays for flavonoid detection in food or plant samples. Our assay design was based on colorimetric flavonoid assays, where the assay reported herein was optimized. Specifically, the flavonoid rutin concentration and sample-to-rutin volume ratio (295:5 µL) were optimized to detect Al3+ at low µM concentrations in samples. The assay performed comparably, and in some instances better, than those requiring advanced instrumentation and previously reported colorimetric assays, with a linear range (1–8 µM), sensitivity (7.6 nM), limit of detection (79.8 nM), and limit of quantification (266 nM) for Al3+. The colorimetric assay was accurate (99 ≤ 108 ± 4 ≤ 6% Al3+ recovery), precise (low intra- and inter-assay coefficient of variation (CV) of 3.1 ≤ 5.9% and 4.4%, respectively), and selective for Al3+ ions compared to solutions containing a variety of other mono-, di-, and tri-cations at much higher concentrations (10- to 100-fold higher). Lastly, the colorimetric assay was applicable to complex analysis. It was used to generate a chelation curve depicting the Al3+ chelation capacity of sodium alginate, a biologically derived polymer used as a bioink for 3D bioprinting.

Стилі APA, Harvard, Vancouver, ISO та ін.

17

Teixeira, Maria C., Nicole S. Lameirinhas, João P. F. Carvalho, Armando J. D. Silvestre, Carla Vilela, and Carmen S. R. Freire. "A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications." International Journal of Molecular Sciences 23, no. 12 (June 12, 2022): 6564. http://dx.doi.org/10.3390/ijms23126564.

Повний текст джерела

Анотація:

Three-dimensional (3D) bioprinting is an innovative technology in the biomedical field, allowing the fabrication of living constructs through an approach of layer-by-layer deposition of cell-laden inks, the so-called bioinks. An ideal bioink should possess proper mechanical, rheological, chemical, and biological characteristics to ensure high cell viability and the production of tissue constructs with dimensional stability and shape fidelity. Among the several types of bioinks, hydrogels are extremely appealing as they have many similarities with the extracellular matrix, providing a highly hydrated environment for cell proliferation and tunability in terms of mechanical and rheological properties. Hydrogels derived from natural polymers, and polysaccharides, in particular, are an excellent platform to mimic the extracellular matrix, given their low cytotoxicity, high hydrophilicity, and diversity of structures. In fact, polysaccharide-based hydrogels are trendy materials for 3D bioprinting since they are abundant and combine adequate physicochemical and biomimetic features for the development of novel bioinks. Thus, this review portrays the most relevant advances in polysaccharide-based hydrogel bioinks for 3D bioprinting, focusing on the last five years, with emphasis on their properties, advantages, and limitations, considering polysaccharide families classified according to their source, namely from seaweed, higher plants, microbial, and animal (particularly crustaceans) origin.

Стилі APA, Harvard, Vancouver, ISO та ін.

18

Mohd, Nurulhuda, Masfueh Razali, Mariyam Jameelah Ghazali, and Noor Hayaty Abu Kasim. "Current Advances of Three-Dimensional Bioprinting Application in Dentistry: A Scoping Review." Materials 15, no. 18 (September 15, 2022): 6398. http://dx.doi.org/10.3390/ma15186398.

Повний текст джерела

Анотація:

Three-dimensional (3D) bioprinting technology has emerged as an ideal approach to address the challenges in regenerative dentistry by fabricating 3D tissue constructs with customized complex architecture. The dilemma with current dental treatments has led to the exploration of this technology in restoring and maintaining the function of teeth. This scoping review aims to explore 3D bioprinting technology together with the type of biomaterials and cells used for dental applications. Based on PRISMA-ScR guidelines, this systematic search was conducted by using the following databases: Ovid, PubMed, EBSCOhost and Web of Science. The inclusion criteria were (i) cell-laden 3D-bioprinted construct; (ii) intervention to regenerate dental tissue using bioink, which incorporates living cells or in combination with biomaterial; and (iii) 3D bioprinting for dental applications. A total of 31 studies were included in this review. The main 3D bioprinting technique was extrusion-based approach. Novel bioinks in use consist of different types of natural and synthetic polymers, decellularized extracellular matrix and spheroids with encapsulated mesenchymal stem cells, and have shown promising results for periodontal ligament, dentin, dental pulp and bone regeneration application. However, 3D bioprinting in dental applications, regrettably, is not yet close to being a clinical reality. Therefore, further research in fabricating ideal bioinks with implantation into larger animal models in the oral environment is very much needed for clinical translation.

Стилі APA, Harvard, Vancouver, ISO та ін.

19

Malekpour, Ali, and Xiongbiao Chen. "Printability and Cell Viability in Extrusion-Based Bioprinting from Experimental, Computational, and Machine Learning Views." Journal of Functional Biomaterials 13, no. 2 (April 10, 2022): 40. http://dx.doi.org/10.3390/jfb13020040.

Повний текст джерела

Анотація:

Extrusion bioprinting is an emerging technology to apply biomaterials precisely with living cells (referred to as bioink) layer by layer to create three-dimensional (3D) functional constructs for tissue engineering. Printability and cell viability are two critical issues in the extrusion bioprinting process; printability refers to the capacity to form and maintain reproducible 3D structure and cell viability characterizes the amount or percentage of survival cells during printing. Research reveals that both printability and cell viability can be affected by various parameters associated with the construct design, bioinks, and bioprinting process. This paper briefly reviews the literature with the aim to identify the affecting parameters and highlight the methods or strategies for rigorously determining or optimizing them for improved printability and cell viability. This paper presents the review and discussion mainly from experimental, computational, and machine learning (ML) views, given their promising in this field. It is envisioned that ML will be a powerful tool to advance bioprinting for tissue engineering.

Стилі APA, Harvard, Vancouver, ISO та ін.

20

Morgan, Francis L. C., Lorenzo Moroni, and Matthew B. Baker. "Dynamic Bioinks to Advance Bioprinting." Advanced Healthcare Materials 9, no. 15 (February 26, 2020): 1901798. http://dx.doi.org/10.1002/adhm.201901798.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

21

Naghieh, Saman, Gabriella Lindberg, Maryam Tamaddon, and Chaozong Liu. "Biofabrication Strategies for Musculoskeletal Disorders: Evolution towards Clinical Applications." Bioengineering 8, no. 9 (September 10, 2021): 123. http://dx.doi.org/10.3390/bioengineering8090123.

Повний текст джерела

Анотація:

Biofabrication has emerged as an attractive strategy to personalise medical care and provide new treatments for common organ damage or diseases. While it has made impactful headway in e.g., skin grafting, drug testing and cancer research purposes, its application to treat musculoskeletal tissue disorders in a clinical setting remains scarce. Albeit with several in vitro breakthroughs over the past decade, standard musculoskeletal treatments are still limited to palliative care or surgical interventions with limited long-term effects and biological functionality. To better understand this lack of translation, it is important to study connections between basic science challenges and developments with translational hurdles and evolving frameworks for this fully disruptive technology that is biofabrication. This review paper thus looks closely at the processing stage of biofabrication, specifically at the bioinks suitable for musculoskeletal tissue fabrication and their trends of usage. This includes underlying composite bioink strategies to address the shortfalls of sole biomaterials. We also review recent advances made to overcome long-standing challenges in the field of biofabrication, namely bioprinting of low-viscosity bioinks, controlled delivery of growth factors, and the fabrication of spatially graded biological and structural scaffolds to help biofabricate more clinically relevant constructs. We further explore the clinical application of biofabricated musculoskeletal structures, regulatory pathways, and challenges for clinical translation, while identifying the opportunities that currently lie closest to clinical translation. In this article, we consider the next era of biofabrication and the overarching challenges that need to be addressed to reach clinical relevance.

Стилі APA, Harvard, Vancouver, ISO та ін.

22

Ojeda, Edilberto, África García-Barrientos, Nagore Martínez de Cestafe, José María Alonso, Raúl Pérez-González, and Virginia Sáez-Martínez. "Nanometric Hydroxyapatite Particles as Active Ingredient for Bioinks: A Review." Macromol 2, no. 1 (January 4, 2022): 20–29. http://dx.doi.org/10.3390/macromol2010002.

Повний текст джерела

Анотація:

Additive manufacturing (AM), frequently cited as three-dimensional (3D) printing, is a relatively new manufacturing technique for biofabrication, also called 3D manufacture with biomaterials and cells. Recent advances in this field will facilitate further improvement of personalized healthcare solutions. In this regard, tailoring several healthcare products such as implants, prosthetics, and in vitro models, would have been extraordinarily arduous beyond these technologies. Three-dimensional-printed structures with a multiscale porosity are very interesting manufacturing processes in order to boost the capability of composite scaffolds to generate bone tissue. The use of biomimetic hydroxyapatite as the main active ingredient for bioinks is a helpful approach to obtain these advanced materials. Thus, 3D-printed biomimetic composite designs may produce supplementary biological and physical benefits. Three-dimensional bioprinting may turn to be a bright solution for regeneration of bone tissue as it enables a proper spatio-temporal organization of cells in scaffolds. Different types of bioprinting technologies and essential parameters which rule the applicability of bioinks are discussed in this review. Special focus is made on hydroxyapatite as an active ingredient for bioinks design. The goal of such bioinks is to reduce the constraints of commonly applied treatments by enhancing osteoinduction and osteoconduction, which seems to be exceptionally promising for bone regeneration.

Стилі APA, Harvard, Vancouver, ISO та ін.

23

Henrionnet, Christel, Léa Pourchet, Paul Neybecker, Océane Messaoudi, Pierre Gillet, Damien Loeuille, Didier Mainard, Christophe Marquette, and Astrid Pinzano. "Combining Innovative Bioink and Low Cell Density for the Production of 3D-Bioprinted Cartilage Substitutes: A Pilot Study." Stem Cells International 2020 (January 21, 2020): 1–16. http://dx.doi.org/10.1155/2020/2487072.

Повний текст джерела

Анотація:

3D bioprinting offers interesting opportunities for 3D tissue printing by providing living cells with appropriate scaffolds with a dedicated structure. Biological advances in bioinks are currently promising for cell encapsulation, particularly that of mesenchymal stem cells (MSCs). We present herein the development of cartilage implants by 3D bioprinting that deliver MSCs encapsulated in an original bioink at low concentration. 3D-bioprinted constructs (10×10×4 mm) were printed using alginate/gelatin/fibrinogen bioink mixed with human bone marrow MSCs. The influence of the bioprinting process and chondrogenic differentiation on MSC metabolism, gene profiles, and extracellular matrix (ECM) production at two different MSC concentrations (1 million or 2 million cells/mL) was assessed on day 28 (D28) by using MTT tests, real-time RT-PCR, and histology and immunohistochemistry, respectively. Then, the effect of the environment (growth factors such as TGF-β1/3 and/or BMP2 and oxygen tension) on chondrogenicity was evaluated at a 1 M cell/mL concentration on D28 and D56 by measuring mitochondrial activity, chondrogenic gene expression, and the quality of cartilaginous matrix synthesis. We confirmed the safety of bioextrusion and gelation at concentrations of 1 million and 2 million MSC/mL in terms of cellular metabolism. The chondrogenic effect of TGF-β1 was verified within the substitute on D28 by measuring chondrogenic gene expression and ECM synthesis (glycosaminoglycans and type II collagen) on D28. The 1 M concentration represented the best compromise. We then evaluated the influence of various environmental factors on the substitutes on D28 (differentiation) and D56 (synthesis). Chondrogenic gene expression was maximal on D28 under the influence of TGF-β1 or TGF-β3 either alone or in combination with BMP-2. Hypoxia suppressed the expression of hypertrophic and osteogenic genes. ECM synthesis was maximal on D56 for both glycosaminoglycans and type II collagen, particularly in the presence of a combination of TGF-β1 and BMP-2. Continuous hypoxia did not influence matrix synthesis but significantly reduced the appearance of microcalcifications within the extracellular matrix. The described strategy is very promising for 3D bioprinting by the bioextrusion of an original bioink containing a low concentration of MSCs followed by the culture of the substitutes in hypoxic conditions under the combined influence of TGF-β1 and BMP-2.

Стилі APA, Harvard, Vancouver, ISO та ін.

24

Ratner, Buddy D. "Biomaterials: Been There, Done That, and Evolving into the Future." Annual Review of Biomedical Engineering 21, no. 1 (June 4, 2019): 171–91. http://dx.doi.org/10.1146/annurev-bioeng-062117-120940.

Повний текст джерела

Анотація:

Biomaterials as we know them today had their origins in the late 1940s with off-the-shelf commercial polymers and metals. The evolution of materials for medical applications from these simple origins has been rapid and impactful. This review relates some of the early history; addresses concerns after two decades of development in the twenty-first century; and discusses how advanced technologies in both materials science and biology will address concerns, advance materials used at the biointerface, and improve outcomes for patients.

Стилі APA, Harvard, Vancouver, ISO та ін.

25

Del Amo, Cristina, Arantza Perez-Valle, Miguel Perez-Garrastachu, Ines Jauregui, Noelia Andollo, Jon Arluzea, Pedro Guerrero, Koro de la Caba, and Isabel Andia. "Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings." Biomedicines 9, no. 8 (August 16, 2021): 1023. http://dx.doi.org/10.3390/biomedicines9081023.

Повний текст джерела

Анотація:

Extrusion bioprinting based on the development of novel bioinks offers the possibility of manufacturing clinically useful tools for wound management. In this study, we show the rheological properties and printability outcomes of two advanced dressings based on platelet-rich plasma (PRP) and platelet-poor plasma (PPP) blended with alginate and loaded with dermal fibroblasts. Measurements taken at 1 h, 4 days, and 18 days showed that both the PRP- and PPP-based dressings retain plasma and platelet proteins, which led to the upregulation of angiogenic and immunomodulatory proteins by embedded fibroblasts (e.g., an up to 69-fold increase in vascular endothelial growth factor (VEGF), an up to 188-fold increase in monocyte chemotactic protein 1 (MCP-1), and an up to 456-fold increase in hepatocyte growth factor (HGF) 18 days after printing). Conditioned media harvested from both PRP and PPP constructs stimulated the proliferation of human umbilical vein endothelial cells (HUVECs), whereas only those from PRP dressings stimulated HUVEC migration, which correlated with the VEGF/MCP-1 and VEGF/HGF ratios. Similarly, the advanced dressings increased the level of interleukin-8 and led to a four-fold change in the level of extracellular matrix protein 1. These findings suggest that careful selection of plasma formulations to fabricate wound dressings can enable regulation of the molecular composition of the microenvironment, as well as paracrine interactions, thereby improving the clinical potential of dressings and providing the possibility to tailor each composition to specific wound types and healing stages.

Стилі APA, Harvard, Vancouver, ISO та ін.

26

Chimene, David, Kimberly K. Lennox, Roland R. Kaunas, and Akhilesh K. Gaharwar. "Advanced Bioinks for 3D Printing: A Materials Science Perspective." Annals of Biomedical Engineering 44, no. 6 (May 16, 2016): 2090–102. http://dx.doi.org/10.1007/s10439-016-1638-y.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

27

Xu, Jie, Shuangshuang Zheng, Xueyan Hu, Liying Li, Wenfang Li, Roxanne Parungao, Yiwei Wang, Yi Nie, Tianqing Liu, and Kedong Song. "Advances in the Research of Bioinks Based on Natural Collagen, Polysaccharide and Their Derivatives for Skin 3D Bioprinting." Polymers 12, no. 6 (May 29, 2020): 1237. http://dx.doi.org/10.3390/polym12061237.

Повний текст джерела

Анотація:

The skin plays an important role in protecting the human body, and wound healing must be set in motion immediately following injury or trauma to restore the normal structure and function of skin. The extracellular matrix component of the skin mainly consists of collagen, glycosaminoglycan (GAG), elastin and hyaluronic acid (HA). Recently, natural collagen, polysaccharide and their derivatives such as collagen, gelatin, alginate, chitosan and pectin have been selected as the matrix materials of bioink to construct a functional artificial skin due to their biocompatible and biodegradable properties by 3D bioprinting, which is a revolutionary technology with the potential to transform both research and medical therapeutics. In this review, we outline the current skin bioprinting technologies and the bioink components for skin bioprinting. We also summarize the bioink products practiced in research recently and current challenges to guide future research to develop in a promising direction. While there are challenges regarding currently available skin bioprinting, addressing these issues will facilitate the rapid advancement of 3D skin bioprinting and its ability to mimic the native anatomy and physiology of skin and surrounding tissues in the future.

Стилі APA, Harvard, Vancouver, ISO та ін.

28

Shakiba, Amin, Oussama Zenasni, Maria D. Marquez, and T. Randall Lee. "Advanced drug delivery via self-assembled monolayer-coated nanoparticles." AIMS Bioengineering 4, no. 2 (2017): 275–99. http://dx.doi.org/10.3934/bioeng.2017.2.275.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

29

Bakht, Syeda M., Alberto Pardo, Manuel Gómez-Florit, Rui L. Reis, Rui M. A. Domingues, and Manuela E. Gomes. "Engineering next-generation bioinks with nanoparticles: moving from reinforcement fillers to multifunctional nanoelements." Journal of Materials Chemistry B 9, no. 25 (2021): 5025–38. http://dx.doi.org/10.1039/d1tb00717c.

Повний текст джерела

Анотація:

The incorporation of nanoparticles is an emerging strategy to develop advanced nanocomposite bioinks with (multi) functional properties that improve the bioactivity and regenerative potential of 3D bioprinted constructs.

Стилі APA, Harvard, Vancouver, ISO та ін.

30

Alizadeh, Parvin, Mohammad Soltani, Rumeysa Tutar, Ehsanul Hoque Apu, Chima V. Maduka, Bige Deniz Unluturk, Christopher H. Contag, and Nureddin Ashammakhi. "Use of electroconductive biomaterials for engineering tissues by 3D printing and 3D bioprinting." Essays in Biochemistry 65, no. 3 (August 2021): 441–66. http://dx.doi.org/10.1042/ebc20210003.

Повний текст джерела

Анотація:

Abstract Existing methods of engineering alternatives to restore or replace damaged or lost tissues are not satisfactory due to the lack of suitable constructs that can fit precisely, function properly and integrate into host tissues. Recently, three-dimensional (3D) bioprinting approaches have been developed to enable the fabrication of pre-programmed synthetic tissue constructs that have precise geometries and controlled cellular composition and spatial distribution. New bioinks with electroconductive properties have the potential to influence cellular fates and function for directed healing of different tissue types including bone, heart and nervous tissue with the possibility of improved outcomes. In the present paper, we review the use of electroconductive biomaterials for the engineering of tissues via 3D printing and 3D bioprinting. Despite significant advances, there remain challenges to effective tissue replacement and we address these challenges and describe new approaches to advanced tissue engineering.

Стилі APA, Harvard, Vancouver, ISO та ін.

31

Valdastri, Pietro, Massimiliano Simi, and Robert J. Webster. "Advanced Technologies for Gastrointestinal Endoscopy." Annual Review of Biomedical Engineering 14, no. 1 (August 15, 2012): 397–429. http://dx.doi.org/10.1146/annurev-bioeng-071811-150006.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

32

Genova, Tullio, Ilaria Roato, Massimo Carossa, Chiara Motta, Davide Cavagnetto, and Federico Mussano. "Advances on Bone Substitutes through 3D Bioprinting." International Journal of Molecular Sciences 21, no. 19 (September 23, 2020): 7012. http://dx.doi.org/10.3390/ijms21197012.

Повний текст джерела

Анотація:

Reconstruction of bony defects is challenging when conventional grafting methods are used because of their intrinsic limitations (biological cost and/or biological properties). Bone regeneration techniques are rapidly evolving since the introduction of three-dimensional (3D) bioprinting. Bone tissue engineering is a branch of regenerative medicine that aims to find new solutions to treat bone defects, which can be repaired by 3D printed living tissues. Its aim is to overcome the limitations of conventional treatment options by improving osteoinduction and osteoconduction. Several techniques of bone bioprinting have been developed: inkjet, extrusion, and light-based 3D printers are nowadays available. Bioinks, i.e., the printing materials, also presented an evolution over the years. It seems that these new technologies might be extremely promising for bone regeneration. The purpose of the present review is to give a comprehensive summary of the past, the present, and future developments of bone bioprinting and bioinks, focusing the attention on crucial aspects of bone bioprinting such as selecting cell sources and attaining a viable vascularization within the newly printed bone. The main bioprinters currently available on the market and their characteristics have been taken into consideration, as well.

Стилі APA, Harvard, Vancouver, ISO та ін.

33

Hartung, Mara Lena, Ronny Baber, Esther Herpel, Cornelia Specht, Daniel Peer Brucker, Anne Schoneberg, Theresa Winter, and Sara Yasemin Nussbeck. "Harmonization of Biobank Education for Biobank Technicians: Identification of Learning Objectives." BioTech 10, no. 2 (April 14, 2021): 7. http://dx.doi.org/10.3390/biotech10020007.

Повний текст джерела

Анотація:

The quality of biospecimens stored in a biobank depends tremendously on the technical personnel responsible for processing, storage, and release of biospecimens. Adequate training of these biobank employees would allow harmonization of correct sample handling and thus ensure a high and comparable quality of samples across biobank locations. However, in Germany there are no specific training opportunities for technical biobank staff. To understand the educational needs of the technical personnel a web-based survey was sent to all national biobanks via established e-mail registers. In total, 79 biobank employees completed the survey, including 43 technicians. The majority of the participating technical personnel stated that they had worked in a biobank for less than three years and had never participated in an advanced training. Three-quarters of the technicians indicated that they were not able to understand English content instantly. Based on these results and the results of a workshop with 16 biobank technicians, 41 learning objectives were formulated. These learning objectives can be used as a basis for advanced training programs for technical personnel in biobanks. Setting up courses based on the identified learning objectives for this group of biobank staff could contribute to harmonization and sustainability of biospecimen quality.

Стилі APA, Harvard, Vancouver, ISO та ін.

34

Lu, Dezhi, Yang Liu, Wentao Li, Hongshi Ma, Tao Li, Xiaojun Ma, Yuanqing Mao, Qianqian Liang, Zhenjiang Ma, and Jinwu Wang. "Development and Application of 3D Bioprinted Scaffolds Supporting Induced Pluripotent Stem Cells." BioMed Research International 2021 (September 13, 2021): 1–13. http://dx.doi.org/10.1155/2021/4910816.

Повний текст джерела

Анотація:

Three-dimensional (3D) bioprinting is a revolutionary technology that replicates 3D functional living tissue scaffolds in vitro by controlling the layer-by-layer deposition of biomaterials and enables highly precise positioning of cells. With the development of this technology, more advanced research on the mechanisms of tissue morphogenesis, clinical drug screening, and organ regeneration may be pursued. Because of their self-renewal characteristics and multidirectional differentiation potential, induced pluripotent stem cells (iPSCs) have outstanding advantages in stem cell research and applications. In this review, we discuss the advantages of different bioinks containing human iPSCs that are fabricated by using 3D bioprinting. In particular, we focus on the ability of these bioinks to support iPSCs and promote their proliferation and differentiation. In addition, we summarize the applications of 3D bioprinting with iPSC-containing bioinks and put forward new views on the current research status.

Стилі APA, Harvard, Vancouver, ISO та ін.

35

Jia, Tao, Yixuan Xiao, Zhonghao Ji, Run Wang, and Jiang Wu. "Recent advances in BiOIO3 based photocatalytic nanomaterials." E3S Web of Conferences 118 (2019): 01043. http://dx.doi.org/10.1051/e3sconf/201911801043.

Повний текст джерела

Анотація:

Semiconductor photocatalysis technology using solar energy has broad application prospects in energy conversion and environmental purification, while the photocatalytic efficiency is unsatisfactory due to limited photoresponse and recombination of charge carriers. Many researchers have made great efforts to develop new and efficient photocatalysts to solve the above problems. Among photocatalysts, bismuth-based nanomaterials have become ideal photocatalysts because of its unique layered structure, narrow band gap, better visible light response and good electron-hole separation characteristics. Particularly, appropriately modified photocatalysts shows better photocatalytic performance due to enhanced photoresponse characteristics and promoted separation and migration of electron-hole pairs. In this review, the characteristics of bismuth oxyiodate in bismuth-based photocatalysts are systematically introduced. Recent efforts for various modification strategies of bismuth oxyiodate have also been demonstrated and evaluated. Finally, this paper also briefly summarizes and forecasts the challenges and development directions of bismuth-based photocatalysts.

Стилі APA, Harvard, Vancouver, ISO та ін.

36

Roche, Christopher D., Russell J. L. Brereton, Anthony W. Ashton, Christopher Jackson, and Carmine Gentile. "Current challenges in three-dimensional bioprinting heart tissues for cardiac surgery." European Journal of Cardio-Thoracic Surgery 58, no. 3 (May 11, 2020): 500–510. http://dx.doi.org/10.1093/ejcts/ezaa093.

Повний текст джерела

Анотація:

Abstract Summary Previous attempts in cardiac bioengineering have failed to provide tissues for cardiac regeneration. Recent advances in 3-dimensional bioprinting technology using prevascularized myocardial microtissues as ‘bioink’ have provided a promising way forward. This review guides the reader to understand why myocardial tissue engineering is difficult to achieve and how revascularization and contractile function could be restored in 3-dimensional bioprinted heart tissue using patient-derived stem cells.

Стилі APA, Harvard, Vancouver, ISO та ін.

37

Lee, Siseon, and Robert J. Mitchell. "Perspectives on the use of transcriptomics to advance biofuels." AIMS Bioengineering 2, no. 4 (2015): 487–506. http://dx.doi.org/10.3934/bioeng.2015.4.487.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

38

Athukorala, Sandya Shiranthi, Tuan Sang Tran, Rajkamal Balu, Vi Khanh Truong, James Chapman, Naba Kumar Dutta, and Namita Roy Choudhury. "3D Printable Electrically Conductive Hydrogel Scaffolds for Biomedical Applications: A Review." Polymers 13, no. 3 (February 2, 2021): 474. http://dx.doi.org/10.3390/polym13030474.

Повний текст джерела

Анотація:

Electrically conductive hydrogels (ECHs), an emerging class of biomaterials, have garnered tremendous attention due to their potential for a wide variety of biomedical applications, from tissue-engineered scaffolds to smart bioelectronics. Along with the development of new hydrogel systems, 3D printing of such ECHs is one of the most advanced approaches towards rapid fabrication of future biomedical implants and devices with versatile designs and tuneable functionalities. In this review, an overview of the state-of-the-art 3D printed ECHs comprising conductive polymers (polythiophene, polyaniline and polypyrrole) and/or conductive fillers (graphene, MXenes and liquid metals) is provided, with an insight into mechanisms of electrical conductivity and design considerations for tuneable physiochemical properties and biocompatibility. Recent advances in the formulation of 3D printable bioinks and their practical applications are discussed; current challenges and limitations of 3D printing of ECHs are identified; new 3D printing-based hybrid methods for selective deposition and fabrication of controlled nanostructures are highlighted; and finally, future directions are proposed.

Стилі APA, Harvard, Vancouver, ISO та ін.

39

Masri, Syafira, and Mh Busra Fauzi. "Current Insight of Printability Quality Improvement Strategies in Natural-Based Bioinks for Skin Regeneration and Wound Healing." Polymers 13, no. 7 (March 25, 2021): 1011. http://dx.doi.org/10.3390/polym13071011.

Повний текст джерела

Анотація:

Skin tissue engineering aimed to replace chronic tissue injury commonly occurred due to severe burn and chronic wound in diabetic ulcer patients. The normal skin is unable to be regenerated until the seriously injured tissue is disrupted and losing its function. 3D-bioprinting has been one of the effective methods for scaffold fabrication and is proven to replace the conventional method, which reported several drawbacks. In light of this, researchers have developed a new fabrication approach via 3D-bioprinting by combining biomaterials (bioinks) with cells and biomolecules followed by a suitable crosslinking approach. This advanced technology has been subcategorised into three different printing techniques including inject-based, laser-based, and extrusion-based printing. However, the printable quality of the currently available bioinks demonstrated shortcomings in the physicochemical and mechanical properties. This review aims to identify the limitations raised by using natural-based bioinks and the optimum temperature for various applied printing techniques. It is essential to ensure maintaining the acceptable printed scaffold property such as the optimum pore sizes and porosity that allow cell migration activity. In addition, the properties required for an ideal bioinks design for better scaffold printability were also summarised.

Стилі APA, Harvard, Vancouver, ISO та ін.

40

Duarte Campos, Daniela F., Andrea Bonnin Marquez, Cathal O’Seanain, Horst Fischer, Andreas Blaeser, Michael Vogt, Diana Corallo, and Sanja Aveic. "Exploring Cancer Cell Behavior In Vitro in Three-Dimensional Multicellular Bioprintable Collagen-Based Hydrogels." Cancers 11, no. 2 (February 5, 2019): 180. http://dx.doi.org/10.3390/cancers11020180.

Повний текст джерела

Анотація:

In vitro cancer 3D models are valuable tools to provide mechanistic insight into solid tumor growth, invasion, and drug delivery. The 3D spheroid model of solid tumors has been the most popular cancer model in use until now. However, previous studies have shown that these spheroid models lack sufficient morphological parameters, which may affect their response to chemicals. In this work, we proposed the fabrication of miniaturized 3D cancer models using collagen type I-based bioprintable bioinks. In the context of a mimicking model for advanced neuroblastoma studies, we showed that cancer cells contained in bioprintable bioinks formed Homer Wright-like rosettes, maintained their proliferative capacities and produced an equivalent Vimentin-rich matrix unlike that of non-bioprintable bioinks which made for poorer models. In addition, bioprintable bioinks were successfully bioprinted as compartmentalized 3D models in the centimeter scale, which was not feasible using non-bioprintable bioinks. In contrast to non-bioprintable hydrogels, we did not observe contraction in their bioprintable counterparts, which is an advantage for prospective 3D bioprinted models that should attain stable rheological and mechanical properties after bioprinting. By adopting this proposed system for the use of patient-derived primary tumor cells, the approach could be introduced as a first line strategy in precision medicine for testing the response of neuroblastoma cells to drugs, especially when disease progresses rapidly or patients do not respond to actual therapy regimens.

Стилі APA, Harvard, Vancouver, ISO та ін.

41

Tiller, Kathryn E., and Peter M. Tessier. "Advances in Antibody Design." Annual Review of Biomedical Engineering 17, no. 1 (December 7, 2015): 191–216. http://dx.doi.org/10.1146/annurev-bioeng-071114-040733.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

42

Maas, Steve A., Gerard A. Ateshian, and Jeffrey A. Weiss. "FEBio: History and Advances." Annual Review of Biomedical Engineering 19, no. 1 (June 21, 2017): 279–99. http://dx.doi.org/10.1146/annurev-bioeng-071516-044738.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

43

Maan, Zeina, Nadia Z. Masri, and Stephanie M. Willerth. "Smart Bioinks for the Printing of Human Tissue Models." Biomolecules 12, no. 1 (January 15, 2022): 141. http://dx.doi.org/10.3390/biom12010141.

Повний текст джерела

Анотація:

3D bioprinting has tremendous potential to revolutionize the field of regenerative medicine by automating the process of tissue engineering. A significant number of new and advanced bioprinting technologies have been developed in recent years, enabling the generation of increasingly accurate models of human tissues both in the healthy and diseased state. Accordingly, this technology has generated a demand for smart bioinks that can enable the rapid and efficient generation of human bioprinted tissues that accurately recapitulate the properties of the same tissue found in vivo. Here, we define smart bioinks as those that provide controlled release of factors in response to stimuli or combine multiple materials to yield novel properties for the bioprinting of human tissues. This perspective piece reviews the existing literature and examines the potential for the incorporation of micro and nanotechnologies into bioinks to enhance their properties. It also discusses avenues for future work in this cutting-edge field.

Стилі APA, Harvard, Vancouver, ISO та ін.

44

Sarkar, Joyita, Swapnil C. Kamble, and Nilambari C. Kashikar. "Polymeric Bioinks for 3D Hepatic Printing." Chemistry 3, no. 1 (February 1, 2021): 164–81. http://dx.doi.org/10.3390/chemistry3010014.

Повний текст джерела

Анотація:

Three-dimensional (3D) printing techniques have revolutionized the field of tissue engineering. This is especially favorable to construct intricate tissues such as liver, as 3D printing allows for the precise delivery of biomaterials, cells and bioactive molecules in complex geometries. Bioinks made of polymers, of both natural and synthetic origin, have been very beneficial to printing soft tissues such as liver. Using polymeric bioinks, 3D hepatic structures are printed with or without cells and biomolecules, and have been used for different tissue engineering applications. In this review, with the introduction to basic 3D printing techniques, we discuss different natural and synthetic polymers including decellularized matrices that have been employed for the 3D bioprinting of hepatic structures. Finally, we focus on recent advances in polymeric bioinks for 3D hepatic printing and their applications. The studies indicate that much work has been devoted to improvising the design, stability and longevity of the printed structures. Others focus on the printing of tissue engineered hepatic structures for applications in drug screening, regenerative medicine and disease models. More attention must now be diverted to developing personalized structures and stem cell differentiation to hepatic lineage.

Стилі APA, Harvard, Vancouver, ISO та ін.

45

Shabbir Hussain, Murtaza, Gabriel M Rodriguez, Difeng Gao, Michael Spagnuolo, Lauren Gambill, and Mark Blenner. "Recent advances in bioengineering of the oleaginous yeast Yarrowia lipolytica." AIMS Bioengineering 3, no. 4 (2016): 493–514. http://dx.doi.org/10.3934/bioeng.2016.4.493.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

46

Leong, Shye Wei, Shing Cheng Tan, Mohd Noor Norhayati, Mastura Monif, and Si-Yuen Lee. "Effectiveness of Bioinks and the Clinical Value of 3D Bioprinted Glioblastoma Models: A Systematic Review." Cancers 14, no. 9 (April 26, 2022): 2149. http://dx.doi.org/10.3390/cancers14092149.

Повний текст джерела

Анотація:

Many medical applications have arisen from the technological advancement of three-dimensional (3D) bioprinting, including the printing of cancer models for better therapeutic practice whilst imitating the human system more accurately than animal and conventional in vitro systems. The objective of this systematic review is to comprehensively summarise information from existing studies on the effectiveness of bioinks in mimicking the tumour microenvironment of glioblastoma and their clinical value. Based on predetermined eligibility criteria, relevant studies were identified from PubMed, Medline Ovid, Web of Science, Scopus, and ScienceDirect databases. Nineteen articles fulfilled the inclusion criteria and were included in this study. Alginate hydrogels were the most widely used bioinks in bioprinting. The majority of research found that alginate bioinks had excellent biocompatibility and maintained high cell viability. Advanced structural design, as well as the use of multicomponent bioinks, recapitulated the native in vivo morphology more closely and resulted in bioprinted glioblastoma models with higher drug resistance. In addition, 3D cell cultures were superior to monolayer or two-dimensional (2D) cell cultures for the simulation of an optimal tumour microenvironment. To more precisely mimic the heterogenous niche of tumours, future research should focus on bioprinting multicellular and multicomponent tumour models that are suitable for drug screening.

Стилі APA, Harvard, Vancouver, ISO та ін.

47

Leong, Shye Wei, Shing Cheng Tan, Mohd Noor Norhayati, Mastura Monif, and Si-Yuen Lee. "Effectiveness of Bioinks and the Clinical Value of 3D Bioprinted Glioblastoma Models: A Systematic Review." Cancers 14, no. 9 (April 26, 2022): 2149. http://dx.doi.org/10.3390/cancers14092149.

Повний текст джерела

Анотація:

Many medical applications have arisen from the technological advancement of three-dimensional (3D) bioprinting, including the printing of cancer models for better therapeutic practice whilst imitating the human system more accurately than animal and conventional in vitro systems. The objective of this systematic review is to comprehensively summarise information from existing studies on the effectiveness of bioinks in mimicking the tumour microenvironment of glioblastoma and their clinical value. Based on predetermined eligibility criteria, relevant studies were identified from PubMed, Medline Ovid, Web of Science, Scopus, and ScienceDirect databases. Nineteen articles fulfilled the inclusion criteria and were included in this study. Alginate hydrogels were the most widely used bioinks in bioprinting. The majority of research found that alginate bioinks had excellent biocompatibility and maintained high cell viability. Advanced structural design, as well as the use of multicomponent bioinks, recapitulated the native in vivo morphology more closely and resulted in bioprinted glioblastoma models with higher drug resistance. In addition, 3D cell cultures were superior to monolayer or two-dimensional (2D) cell cultures for the simulation of an optimal tumour microenvironment. To more precisely mimic the heterogenous niche of tumours, future research should focus on bioprinting multicellular and multicomponent tumour models that are suitable for drug screening.

Стилі APA, Harvard, Vancouver, ISO та ін.

48

Gao, Ge, Minjun Ahn, Won-Woo Cho, Byoung-Soo Kim, and Dong-Woo Cho. "3D Printing of Pharmaceutical Application: Drug Screening and Drug Delivery." Pharmaceutics 13, no. 9 (August 31, 2021): 1373. http://dx.doi.org/10.3390/pharmaceutics13091373.

Повний текст джерела

Анотація:

Advances in three-dimensional (3D) printing techniques and the development of tailored biomaterials have facilitated the precise fabrication of biological components and complex 3D geometrics over the past few decades. Moreover, the notable growth of 3D printing has facilitated pharmaceutical applications, enabling the development of customized drug screening and drug delivery systems for individual patients, breaking away from conventional approaches that primarily rely on transgenic animal experiments and mass production. This review provides an extensive overview of 3D printing research applied to drug screening and drug delivery systems that represent pharmaceutical applications. We classify several elements required by each application for advanced pharmaceutical techniques and briefly describe state-of-the-art 3D printing technology consisting of cells, bioinks, and printing strategies that satisfy requirements. Furthermore, we discuss the limitations of traditional approaches by providing concrete examples of drug screening (organoid, organ-on-a-chip, and tissue/organ equivalent) and drug delivery systems (oral/vaginal/rectal and transdermal/surgical drug delivery), followed by the introduction of recent pharmaceutical investigations using 3D printing-based strategies to overcome these challenges.

Стилі APA, Harvard, Vancouver, ISO та ін.

49

Hyder, Fahmeed, and Douglas L. Rothman. "Advances in Imaging Brain Metabolism." Annual Review of Biomedical Engineering 19, no. 1 (June 21, 2017): 485–515. http://dx.doi.org/10.1146/annurev-bioeng-071516-044450.

Повний текст джерела

Стилі APA, Harvard, Vancouver, ISO та ін.

50

Mladenov, Valeri, and Stoyan Kirilov. "ADVANCED MEMRISTOR MODEL WITH A MODIFIED BIOLEK WINDOW AND A VOLTAGE-DEPENDENT VARIABLE EXPONENT." Informatyka Automatyka Pomiary w Gospodarce i Ochronie Środowiska 8, no. 2 (May 30, 2018): 15–20. http://dx.doi.org/10.5604/01.3001.0012.0697.

Повний текст джерела

Анотація:

The main idea of the present research is to propose a new nonlinear ionic drift memristor model suitable for computer simulations of memristor elements for different voltages. For this purpose, a modified Biolek window function with a voltage-dependent exponent is applied. The proposed modified memristor model is based on Biolek model and due to this and to the use of a voltage-dependent positive integer exponent in the modified Biolek window function it has a new improved property - changing the model nonlinearity extent dependent on the integer exponent in accordance with the memristor voltage. Several computer simulations were made for soft-switching and hard-switching modes and also for pseudo-sinusoidal alternating voltage with an exponentially increasing amplitude and the respective basic important time diagrams, state-flux and i-v relationships are established.

Стилі APA, Harvard, Vancouver, ISO та ін.

Статті в журналах з теми "Advanced bioink"

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями