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Статті в журналах з теми "Activité larvicide":
1
R., ATIKO,, KWAJI , A., and MUSA , A. A. "CHEMICAL CONSTITUENTS AND LARVICIDAL ACTIVITY OF VOLATILE OIL FROM Solenostemon monostachyus (P Beauv.) Briq. AGAINST Anopheles gambiae." BIMA JOURNAL OF SCIENCE AND TECHNOLOGY (2536-6041) 6, no. 02 (August 30, 2022): 102–7. http://dx.doi.org/10.56892/bimajst.v6i02.360.
Анотація:
Synthetic larvicides have been used to control malarial vector. However, increasing resistance of mosquito larvae to the synthetic larvicides poses great problem in the vector control. Natural control with plant essential oil may serve as an alternative. This study investigates the chemical constituents and larvicidal activity of volatile oil obtained from Solenostemon monostachyusagainst the larvae of Anopheles gambiae. The volatile oils were extracted using hydro-distillation in a Clevenger type apparatus and analyzed using GC-MS. The larvicidal assay was carried out against Anopheles gambiae larvae at concentration range of 12.5- 400 μg/mL. The analysis of the volatile oil showed that the major constituents of the oil were Caryophyllene oxide (21.6%), β-Caryophyllene (19.6%), β-Pinene (9.8%) and Germacrene D (7.3%). Results showed that larval mortality increases with increasing exposure period to the volatile oils from S. monostachyus. The larvicidal activity demonstrated that the mosquito larvae were susceptible to the volatile oil with LC50 of 23.44 μg/mL. The results suggest that S. monostachyus essential oil has potentials for the control of Anopheles gambiae. Therefore the plant may serve as a potential source of raw material for a new and eco-friendly larvicide.
2
Manzano, Patricia, Oscar Valmaña García, Jessica Malusín, Jenny Villamar, María Quijano, Rafael Viteri, Ana Barragán, and Andrea Orellana-Manzano. "Larvicidal activity of ethanolic extract of Azadirachta indica against Aedes aegypti larvae." Revista Facultad Nacional de Agronomía Medellín 73, no. 3 (September 1, 2020): 9315–20. http://dx.doi.org/10.15446/rfnam.v73n3.80501.
Анотація:
Aedes aegypti is a mosquito that carries dengue virus, yellow fever and other diseases transmitted to humans. Organophosphorus larvicides are used to control the proliferation of this mosquito, which has generated a high degree of resistance; hence, new alternatives such as bio-larvicides formulated with plant extracts are of great interest. The aims of this study were to evaluate the ethanolic extract of Azadirachta indica leaves as a larvicide against Aedes aegypti and to determine the main compounds present in it by GC-MS. In the assay, three concentrations of ethanolic extract were used (10 mg L-1, 20 mg L-1, and 50 mg L-1). This was performed thrice against a positive control (commercial larvicide: spores and endotoxic crystals of Bacillus thuringiensis var. israelensis Serotype H-14) and negative control (water). After 72 h of incubation, it was observed higher larval mortality (93%) in the ethanolic extract at a concentration of 50 mg L-1; the extracts at 10 mg L-1 and 20 mg L-1 shown larval mortality of 47% and 70%, respectively. The majority compound determined by the GC-MS analysis was phytol (14.4% area). The results obtained in this study demonstrated the larvicidal potential of the ethanolic extract of A. indica against larvae of A. aegypti.
3
Widodo, Muhammad Noer Perdana Sakti, Indria Augustina, Elsa Trinovita, Ratna Widayati, and Arif Rahman Jabal. "Studi Literatur: Potensi Daya Bunuh Tanaman Keluarga Asteraceae Terhadap Larva Aedes Aegypti." Jurnal Surya Medika 9, no. 2 (August 27, 2023): 243–56. http://dx.doi.org/10.33084/jsm.v9i2.3837.
Анотація:
Aedes aegypti is the main transmitter of dengue hemorrhagic fever which is an important health problem in Indonesia and the world. The main vector of dengue hemorrhagic fever (DHF) is the Aedes aegypti mosquito which originated from Africa and discovered in Indonesia in 1968 in Surabaya. DHF continues to spread to all 472 districts or cities in 34 provinces in Indonesia. Data on dengue cases recorded until the 49th week of 2020 were 95,893 cases and 661 cases of death. Biological control using natural ingredients which is Asteraceae family plants that have larvicidal activity is one of the methode to prevent transmission and reproduction of the main vector. The object of this study is to know the potential of Asteraceae family plants as larvicides against Aedes aegypti. The method of this study is systematic review. The data sources used are secondary data obtained from Google Scholar. The keywords are Asteraceae, Aedes aegypti and larvicide. There are 17 literatures which fulfill the criteria. Synthesis of data is using SPIDER. The result of this study is, from 17 international and national journals reviewed, there were 9 (52,95%) journals shows that Asteraceae family plants have low potential to become Aedes aegypti larvicides and 8 (47,05%) journals shows that Asteraceae family plant have the potential to become Aedes aegypti larvicides, this is based on the LC50 value >1000ppm which indicates that the larvicidal activity is low. Asteraceae family plants have low potential to become Aedes aegypti larvicides.
4
Chan, Chee Aun, Lai Yee Ho, and Nam Weng Sit. "Larvicidal Activity and Phytochemical Profiling of Sweet Basil (Ocimum basilicum L.) Leaf Extract against Asian Tiger Mosquito (Aedes albopictus)." Horticulturae 8, no. 5 (May 16, 2022): 443. http://dx.doi.org/10.3390/horticulturae8050443.
Анотація:
Applying larvicides to interrupt a mosquito’s life cycle is an important strategy for vector control. This study was conducted to evaluate the larvicidal properties of the hexane extract of sweet basil (Ocimum basilicum L.; family Lamiaceae) leaves against the wild strain of Asian tiger mosquito, Aedes albopictus (Skuse). Third instar larvae (20 larvae/replicate, n = 3) were exposed to different concentrations of the extract (6.25–200 µg/mL), and the mortality rate was recorded. Probit analysis showed that the median lethal concentration and 95% lethal concentration of the extract were 16.0 (10.9–22.1) and 53.0 (34.6–136.8) µg/mL, respectively, after 24 h exposure. Only the fractions F3, F4, and F5 from the column chromatography displayed high mortality rates of 91.7–100% at 25.0 µg/mL after 24 h exposure. Subsequent column chromatography from the pooled fraction yielded two active subfractions, H-F345-S2 and H-F345-S3, with mortality rates of 100% and 98.3 ± 2.9%, respectively, at 12.5 µg/mL. Gas chromatography–mass spectrometry analysis unveiled that methyl chavicol, 2-(2-butoxyethoxy)ethanol, cedrelanol, methyl eugenol, 2,4,di-tert-butylphenol, and phytol were the major components in both subfractions with some of them being reported as larvicidal compounds. The results suggest that sweet basil has substantial larvicidal activity against Ae. albopictus mosquito and is a potential source of naturally derived larvicide.
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Wijaya, Made Dharmesti, Anak Agung Gede Indraningrat, and I. Gede Yoga Ayuning Kirtanayasa. "Phytochemicals and Larvicidal Activity of Sonneratia alba Root Extracts from Ngurah Rai Mangrove Forest, Denpasar-Bali." Biology, Medicine, & Natural Product Chemistry 12, no. 2 (September 9, 2023): 499–505. http://dx.doi.org/10.14421/biomedich.2023.122.499-505.
Анотація:
Dengue is an endemic disease with a high incidence in almost all Southeast Asian countries, including Indonesia. This infectious disease is caused by a virus transmitted by the bite of Aedes aegypti mosquito as the main vector. Effective mosquito vector control is a crucial step in stopping the spread of this virus. Of the several methods available, the use of larvicides is considered one of the most successful treatments in reducing the number of mosquito vectors. However, widely used synthetic larvicides can have undesirable side effects on the environment and non-target organisms including human health. The aim of this study was to discover a new biolarvicide from natural materials that is relatively safer. In this study, the biolarvicidal activity of mangrove plant species that are commonly found in Ngurah Rai Mangrove Forest Bali was tested, namely Sonneratia alba. Mangrove root simplicia was extracted by reflux method using three different solvents namely methanol, chloroform, and n-hexane. The content of compounds in the extract were analyzed using GC/MS method. The larvicidal activity of the extracts were tested on A. aegypti instar III/IV larvae with concentrations of 0.1, 1, 10, 100, and 1000 ppm for 24 hours. Subsequently, the average value of larval mortality was used to calculate the LC50 of each extract. The results showed that S. alba methanol extract provided the best larvicidal activity compared to chloroform and n-hexane extracts, with mortality of 69.33% at a concentration of 1000 ppm and LC50 of 1265 ppm. GC-MS analysis showed that the methanol extract of S. alba contained five dominant compounds namely Methyl 2-hydroxy-eicosanoate (19.55%); 4H-1-Benzopyran-4-one, 3,5-dihydroxy-2-(4-hydroxy-3-methoxyphenyl)-7-methoxy (16.48%); 4-((1E)-3-Hydroxy-1-propenyl)-2-methoxyphenol (10.06%); Benzamide, N-[4-(2-naphtyl)-2-thiazolyl]- (9.40%); and 2,3-Dihydro-3,5-bis(3-methoxyphenyl)-1H-inden-1-one (6.52%). The results of this study provide a preliminary result on larvicide activity from mangrove S. alba in order to develop bio larvicides from nature which is safer for human health and environment.
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Nadio, Nafadjara Abouwaliou, Bigala Gmasson, Essolakina Magnim Bokobana, Pikassalé Akantetou Kantetou, Wiyao Poutaouli, Koffi Koba, and Komla Sanda. "Effet Larvicide de l’Huile Essentielle d’Eucalyptus globulus Labill. (Myrtaceae) sur Pectinophora gossypiella S. et Thaumatotibia leucotreta M. (Lepidoptera), Ravageurs Carpophages du Cotonnier au Togo." European Scientific Journal, ESJ 19, no. 30 (October 31, 2023): 181. http://dx.doi.org/10.19044/esj.2023.v19n30p181.
Анотація:
L’usage massif des pesticides chimiques en protection phytosanitaire présente un danger énorme sur la santé publique et sur l’environnement. Il s’impose de trouver des moyens de protection écologique respectueux de l’environnement. Dans cette étude, l’activité insecticide de l’huile essentielle d’Eucalyptus globulus a été évaluée au laboratoire et en milieu réel contre deux ravageurs importants du cotonnier, Pectinophora gossypiella et Thaumatotibia leucotreta. Les larves de ces ravageurs ont été exposées d’abord à l’effet toxique des différentes concentrations de l’huile essentielle par test de contact et par ingestion au laboratoire. Les taux de mortalité enregistrés ont été comparés aux témoins absolus et de référence. Les résultats ont montré que l’huile essentielle d’E. globulus a exercé une activité insecticide importante sur les larves. En effet, l’huile essentielle a entrainé un taux de mortalité de 100 et 99% à la dose 1µl/ml respectivement sur P. gossypiella et T. leucotreta après 24 heures d’exposition au test de contact. Avec le test d’ingestion, les taux de mortalité ont été 36 et 30,78% respectivement sur P. gossypiella et T. leucotreta. En milieu réel, les résultats des traitements à base des formulations d’huile essentielle d’E. globulus en comparaison avec l’insecticide de référence ont montré que les infestations des populations de P. gossypiella et T. leucotreta ont été plus faibles dans les modalités de traitement à base d’huile essentielle de E. globulus à 1µl/ml (TE3). L’analyse sanitaire des capsules vertes a montré une réduction du nombre de ravageurs de 6,25±1,29 T. leucotreta par plant dans les parcelles témoins absolus à 3,29±0,52 dans les parcelles à traitement TE3. Il a été observé également une réduction du nombre de 9,04±1,38 P. gossypiella par plant (témoin absolu) à 4,13±0,39 (TE3). Les rendements moyens élevés en coton graine ont été de 1933,10±0,29Kg et 1927,08±0,12Kg/ha respectivement avec le témoin référence TV et TE3. Il a été faible dans les parcelles témoins absolus avec 1197,92±0,33Kg/ha. L’extrait d’E. globulus possède une activité insecticide potentielle au laboratoire comme au champ.
The massive use of chemical pesticides in plant protection poses an enormous threat to public health and the environment. We need to find ecological protection methods that respect the environment. In this study, the insecticidal activity of Eucalyptus globulus essential oil was evaluated in the laboratory and in the field against two major cotton pests, Pectinophora gossypiella and Thaumatotibia leucotreta. The larvae of these pests were first exposed to the toxic effect of different concentrations of the essential oil by contact test and by ingestion in the laboratory. The mortality rates recorded were compared with absolute and reference controls. The results showed that the essential oil of E. globulus exerted significant insecticidal activity on the larvae. Indeed, the essential oil caused a mortality rate of 100 and 99% at the dose of 1µl/ml respectively on P. gossypiella and T. leucotreta after 24 hours of exposure to the contact test. With the ingestion test, the mortality rates were 36 and 30.78% respectively on P. gossypiella and T. leucotreta. In the real environment, the results of treatments based on formulations of essential oil of E. globulus in comparison with the reference insecticide showed that infestations of P. gossypiella and T. leucotreta populations were lower in treatment modalities based on essential oil of E. globulus at 1µl/ml (TE3). Health analysis of the green bolls showed a reduction in the number of pests from 6.25±1.29 T. leucotreta per plant in the absolute control plots to 3.29±0.52 in the TE3 treatment plots. There was also a reduction in the number of P. gossypiella from 9.04±1.38 per plant (absolute control) to 4.13±0.39 (TE3). High average yields of seed cotton were 1933.10±0.29Kg and 1927.08±0.12Kg/ha respectively with the TV and TE3 reference controls. It was low in the absolute control plots with 1197.92±0.33Kg/ha. E. globulus extract has potential insecticidal activity both in the laboratory and in the field.
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Banne, Yos, Olfie Sahelangi, Steven Soenjono, Elisabeth Natalia Barung, Selfie Ulaen, Rivolta G. M. Walalangi, and Zulfiayu Sapiun. "Silver Nanoparticle of Acalypha indica Linn. Leaf As Bio-larvicide against Anopheles sp. Larvae." Open Access Macedonian Journal of Medical Sciences 9, A (September 17, 2021): 760–65. http://dx.doi.org/10.3889/oamjms.2021.6628.
Анотація:
BACKGROUND: Acalypha indica Linn. has been used as traditional medicine, it contains flavonoids, alkaloids, tannins, saponins, steroids, triterpenoids, and essential oils. AIM: This study aimed to determine the bio-larvicide effects of A. indica Linn. leaf stew and the silver nanoparticles against Anopheles sp. larvae. METHODS: The fresh leaves of A. indica Linn. extracted using distilled water at 100°C for 30 min. The silver nanoparticles were made by mixing a solution of silver nitrate with the stew, which acts as a reducing agent. The resulting silver nanoparticles were characterized by particle size analyzer and UV-vis spectrophotometer. The bio-larvicide effects against Anopheles sp. larvae performed using a completely randomized design. There were eight groups consisted of ten larvae and three replications. Treatment groups of stew and silver nanoparticle for concentrations 0.05%, 0.5%, and 5%, respectively. The negative control group was distilled water and the positive control group was the 0.01% abate solution. Assessment of larvicide activity was carried out every hour for 6 h and continued if there were larvae that live up to 24 h. The LC50 value was calculated based on Probit analysis. RESULTS: The results showed that the A. indica Linn. leaf stew can be made into silver nanoparticles preparations, optimal results were obtained from a mixture of 1% stew and 3 mM AgNO3. The result of bio-larvicides effect test against Anopheles sp. larvae showed that the LC50 value of the A. indica Linn. leaf stew was 727,3 ppm and the LC50 value of silver nanoparticles was 3.366 ppm. CONCLUSION: It can be concluded that A. indica Linn. is a promising larvicidal plant and can be made into silver nanoparticle preparations.
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Silva, Raquel L., Daniel P. Demarque, Renata G. Dusi, João Paulo B. Sousa, Lorena C. Albernaz, and Laila S. Espindola. "Residual Larvicidal Activity of Quinones against Aedes aegypti." Molecules 25, no. 17 (August 31, 2020): 3978. http://dx.doi.org/10.3390/molecules25173978.
Анотація:
The number of documented dengue cases has increased dramatically in recent years due to transmission through the Aedes aegypti mosquito bite. Vector control remains the most effective measure to protect against this and other arboviral diseases including Zika, chikungunya and (urban) yellow fever, with an established vaccine only available for yellow fever. Although the quinone class shows potential as leading compounds for larvicide development, limited information restricts the development of optimized structures and/or formulations. Thus, in this contribution we investigated the larvicidal and pupicidal activity of three quinone compounds isolated from a Connarus suberosus root wood ethyl acetate extract together with 28 quinones from other sources. Eight quinones demonstrated larvicidal activity, of which tectoquinone (4) proved to be the most active (LC50 1.1 µg/mL). The essential residual effect parameter of four of these quinones was evaluated in laboratory trials, with tectoquinone (4) and 2-ethylanthraquinone (7) presenting the most prolonged activity. In small-scale field residual tests, tectoquinone (4) caused 100% larvae mortality over 5 days, supporting its selection for formulation trials to develop a prototype larvicide to control Ae. aegypti.
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Jorge, Márcia Ramos, Felipe Mendes Merey, Bruno do Amaral Crispim, Fábio Kummrow, Alexeia Barufatti, Fabiana Gomes da Silva Dantas, Kelly Mari Pires de Oliveira, and Eduardo José de Arruda. "Ecotoxicological Evaluation of Products Obtained from Technical Cashew Nutshell Liquid (tCNSL) Proposed as Larvicide to Control Aedes aegypti (Diptera: Culicidae)." Ecologies 3, no. 2 (May 31, 2022): 161–74. http://dx.doi.org/10.3390/ecologies3020013.
Анотація:
The development of new insecticides for vector control that are toxicologically safe and eco-friendly (such as those obtained from industrial by-products) is an important public health concern. Previous research has shown that the obtained tCNSL (technical cashew nutshell liquid) + NatCNSLS (sodium tCNSL sulfonate mixture) emulsion displayed both surfactant properties and larvicidal activity (LC50-24 h 110.6 mg/L). Thus, the emulsion is considered a promising alternative product for the control of Aedes aegypti. The goal of this study was an ecotoxicological evaluation of the tCNSL + NatCNSLS mixture emulsion and its components. In addition, we compared the toxicity of the tCNSL + NatCNSLS mixture emulsion with toxicity data from larvicide currently recommended by the World Health Organization (WHO). Ecotoxicological tests were performed to assess acute toxicity, phytotoxicity, cytotoxicity, genotoxicity, and mutagenicity using Daphnia similis, Pseudokirchneriella subcapitata, Oreochromis niloticus, Allium cepa, and Salmonella enterica serovar Typhimurium. Regarding acute toxicity, D. similis was the most sensitive test organism for the three evaluated products, followed by P. subcapitata and O. niloticus. The highest acute toxicity product was tCNSL. The tCNSL + NatCNSLS mixture emulsion did not show cytotoxic, genotoxic, or mutagenic effects, and showed low acute toxicity to D. similis. In addition, the tCNSL + NatCNSLS mixture emulsion presented a lower or similar toxicological classification to the larvicides recommended by the WHO. Therefore, ecotoxicological tests suggest that the tCNSL + NatCNSLS mixture emulsion can be considered a larvicide environmentally safe way to control Ae. aegypti.
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Ikhsanudin, Azis, Lolita Lolita, and Zayyana Septya Ramadani. "Larvicidal activity of granulated pharmaceutical products using Indonesian holy basil leaf extract." International Journal of Public Health Science (IJPHS) 10, no. 4 (December 1, 2021): 934. http://dx.doi.org/10.11591/ijphs.v10i4.21004.
Анотація:
<em>Ocimum sanctum</em> Linn, known as holy basil, is a larvicide, which is relatively safe compared to synthetic insecticides. This study investigates the larvicidal activity of a granule formulation of Indonesian holy basil leaf extract against third larval instar of <em>Aedes aegypti</em>. The extract of holy basil leaves was obtained by a maceration process with 96% ethanol. The granule was formulated with various concentrations of holy basil leaf extract, including F1 (2000 ppm), F2 (4000 ppm), and F3 (6000 ppm). The extract contained terpenoid, alkaloid, saponin, flavonoid, and polyphenol compounds. The extract granules had a moisture content of 3.01%, flowability of 1.51 seconds, and dispersion time of 1.09 seconds. The mortality rates of mosquitos treated with the different formulation groups were significantly different from positive control with values of 25.33% (F1), 50.67% (F2), and 90.67% (F3). In conclusion, the granulated formulation of holy basil leaf extract has a larvicidal LC<sub>50</sub> of 4405.803 ppm and LC<sub>90</sub> of 6080.714 ppm. Therefore, a granulated pharmaceutical product derived from holy basil leaf extract could be developed as a potent larvicide to control dengue fever.
Дисертації з теми "Activité larvicide":
1
Kouassi, Koffi Marcel. "Comportement et activité larvicide de Bacillus thuringiensis sérovariéte israelensis dans un cours d'eau." Thèse, Université du Québec à Trois-Rivières, 1987. http://depot-e.uqtr.ca/5757/1/000569504.pdf.
2
Ben, Nasr Rania. "Investigation phytochimique, évaluation des activités larvicide et anti-acétylcholinestérase de différents extraits de Mercurialis annua L." Electronic Thesis or Diss., Université de Lorraine, 2021. http://www.theses.fr/2021LORR0155.
Анотація:
Ce travail rapporte le lien entre l'activité larvicide et la composition chimique des différents extraits des pieds mâles et femelles de Mercurialis annua L. une Euphorbiaceae souvent utilisées dans la médecine traditionnelle. Le matériel végétal exploité dans notre étude est ramassé dans quatre régions de la Tunisie situées dans des étages bioclimatiques différents : Bizerte, Jandouba, Nabeul et Beja. Cette étude comprend une première partie consacrée à une synthèse bibliographique. La deuxième partie est consacrée aux expérimentations et résultats. En effet, nous avons évalué l’activité antioxydante des différents extraits à l'aide d'essais DPPH. Les composés phénoliques ont été identifiés et quantifiés par chromatographie liquide couplée à un détecteur UV et à la spectrométrie de masse (LC-UV-ESI / MS). Nos résultats ont montré que les extraits méthanoliques des plantes mâles ont une activité antioxydante (AOA) plus élevé que celle détectée dans les extraits des plantes femelles. D’autre part, les extraits de plantes mâles et femelles de Bizerte ont affiché le taux d'AOA le plus élevé. Pour les deux sexes, les extraits de plantes de Beja ont la plus faible AOA. Certains composés phénoliques tels que : la narcissine, la gallocatéchine, la rutine, l'épigallocatéchine et l'épicatéchine ont été identifiés et quantifiés par LC-MS. D’autre part, l'activité larvicide des différents extraits aqueux de Mercurialis annua L. contre les larves de Tribolium confusum (Du Val) ont montré que la mortalité pouvait atteindre 100% après 48 heures d'exposition pour certains extraits, ceci est confirmé par leur faible LC50. Par ailleurs, l’étude de l’activité anti-acétylcholinestérase des extraits testés montrent que ces derniers sont des inhibiteurs de l’AChE plus puissants que la galantamine utilisée comme contrôle positif. Notre étude a confirmé que des extraits des feuilles et des graines des deux sexes de Mercurialis annua L., provenant des différentes régions de la Tunisie, peuvent donc être exploités pour d’autres activités biologiques, parmi lesquelles l’action inhibitrice de l’AChE En effet ces extraits sont riches en composés phénoliques, ce qui leur confère cette propriété thérapeutique contre la maladie d'Alzheimer et le piégeage les radicaux libresThis work reports the link between the larvicidal activity and the chemical composition of the different extracts of the male and female feet of Mercurialis annua L. a Euphorbiaceae often used in traditional medicine. The plant material used in our study is collected in four regions of Tunisia located in different bioclimatic stages: Bizerte, Jandouba, Nabeul and Beja. This study includes a first part devoted to a bibliographic synthesis. The second part is devoted to experiments and results. Indeed, we evaluated the antioxidant activity of the different extracts using DPPH tests. The phenolic compounds were identified and quantified by liquid chromatography coupled with a UV detector and mass spectrometry (LC-UV-ESI / MS). Our results showed that methanolic extracts from male plants have higher antioxidant activity (AOA) than that detected in extracts from female plants. On the other hand, male and female plant extracts from Bizerte showed the highest AOA level. For both sexes, Beja plant extracts have the lowest AOA. Certain phenolic compounds such as: narcissin, gallocatechin, rutin, epigallocatechin and epicatechin have been identified and quantified by LC-MS. On the other hand, the larvicidal activity of the various aqueous extracts of Mercurialis annua L. against the larvae of Tribolium confusum (Du Val) showed that the mortality could reach 100% after 48 hours of exposure for certain extracts, this is confirmed. by their low LC50. Furthermore, the study of the anti-acetylcholinesterase activity of the extracts tested shows that they are more potent AChE inhibitors than galantamine used as a positive control.Our study confirmed that extracts of the leaves and seeds of both sexes of Mercurialis annua L., coming from different regions of Tunisia, can therefore be exploited for other biological activities, among which the inhibitory action of AChE. Indeed, these extracts are rich in phenolic compounds, which gives them this therapeutic property against Alzheimer's disease and scavenging free radicals
3
Gorssi, Jenifer Carvalho. "Avaliação da toxicidade de tiossemicarbazonas derivadas de benzaldeído, acetofenona e benzofenona frente a larvas de Aedes aegypti." Universidade Federal de Juiz de Fora - Campus Avançado de Governador Valadares, 2018. https://repositorio.ufjf.br/jspui/handle/ufjf/6830.
Анотація:
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As tiossemicarbazonas são uma classe de compostos químicos com amplo perfil farmacológico, contudo há poucos estudos sobre sua toxicidade em insetos. O Aedes aegypti (Linnaeus, 1762) é na atualidade um dos mosquitos que mais tem causado problemas de saúde pública, devido ao seu potencial de disseminar doenças, como dengue, febre amarela, chikungunya e zika. Entre as estratégias mais eficientes no controle dessas doenças está o combate ao vetor. No entanto, o número limitado de agentes larvicidas e o surgimento de mosquitos resistentes podem comprometer esse combate, o que tem motivado a busca por novas moléculas que podem atuar como larvicidas. Neste trabalho foi avaliada a toxicidade de uma série de quatorze tiossemicarbazonas derivadas de benzaldeído, acetofenona e benzofenona contra larvas do Aedes aegypti. Os compostos foram preparados e caracterizados a partir de seus espectros de infravermelho e ressonância magnética nuclear, bem como, por sua estabilidade coloidal em solução aquosa com dimetilsulfóxido 1%. A toxicidade dos compostos em concentrações na faixa de 0,1 a 100 μM foi avaliada frente a larvas no estádio L4, sendo demonstrado que as tiossemicarbazonas derivadas de benzofenona apresentam atividade larvicida. O comportamento de nado e a morfologia externa e das células do intestino médio foram avaliados para os compostos que apresentaram atividade larvicida, sendo observado a redução da mobilidade, deformidade de algumas larvas e alterações nas células após a exposição com tiossemicarbazonas. Ensaios de imunofluorescência utilizando os anticorpos anti-fosfohistona H3, anti-proteína de cadeia leve 3 associada a microtúbulos (anti-LC3A/B), anti-caspase-3 e anti-peroxidase foram realizados nas células do intestino médio das larvas. Esses resultados ressaltaram o efeito citotóxico das tiossemicarbazonas, contudo sem a participação dos processos de apoptose, autofagia e de enzimas peroxidase. Os resultados obtidos corroboram a potencial atividade larvicida das tiossemicarbazonas, mas a origem da sua citotoxicidade deve ser melhor investigada.
The thiosemicarbazones are a class of chemical compounds with broad pharmacological profile, however, there is few studies on its toxicity in insects. Currently, Aedes aegypti mosquitoes (Linnaeus, 1762) has caused public health problems due to its potential for disease dissemination, such as dengue fever, yellow fever, chikungunya and zika. The fight against the vector is one of most efficient strategies used in the control of these diseases. However, the limited number of larvicidal agents and the emergence of resistant mosquitoes can compromise this fight, which has motivated the research for new molecules that can act as larvicides. In this work, a series of fourteen thiosemicarbazones derived from benzaldehyde, acetophenone and benzophenone against Aedes aegypti larvae were evaluated. The compounds were prepared and characterized by their infrared and nuclear magnetic resonance spectra as well as its colloidal stability in 1% DMSO aqueous solution. The larvicidal activity of the compounds, at concentration range from 0.1 to 100 μM, was evaluated against larvae in the L4 stage, being that benzophenone-derived thiosemicarbazones demonstrated larvicidal activity. The swimming behavior and external and midgut cells morphology were evaluated for the active compounds. In these experiments were observed a reduction of mobility, deformity of some larvae and alterations in cells upon exposition to thiosemicarbazones. Immunofluorescence assays involving anti-phospho-histone H3, microtubule-associated protein light chain 3 antibody (anti-LC3A/B), anti-caspase 3, and anti-peroxidase antibodies were performed on midgut cells from larvae. These results highlight the cytotoxic effect of thiosemicarbazones, but without the participation of apoptotic and autophagic processes and peroxidase enzymes. The results obtained corroborate a potential larvicidal activity of thiosemicarbazones, however, the origin of its cytotoxicity should be better investigated.
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Brito, Thaysnara Batista. "Estudos de síntese, toxicidade e relação estrutura-atividade de derivados indólicos 3-substituídos em Aedes aegypti (Diptera: Culicidae) e Artemia sp. (Artemidae)." Pós-Graduação em Ciências Farmacêuticas, 2018. http://ri.ufs.br/jspui/handle/riufs/9125.
Анотація:
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConsidered viral diseases of major re-emerging in the world, the dengue, chikungunya and Zika have as the main vector Aedes aegypti (L.) (Diptera: Culicidae). Larvicidal vector control is an important measure to prevent the transmission of such infections. A major challenge in the control of this arthropod is the low sensitivity of its population through the use of conventional larvicides. Thus, the insect acquires resistance, reducing the effectiveness of these pesticides. Consequently, it will lead to an increase in the risks of toxicity in nontarget organisms and a change in the environment. An alternative to avoid problems caused by the use of these products is the search for new compounds with less environmental impact and better benefits to human health. The indole molecule ring represents one of the subunits of great importance in the discovery of new pesticide products for the pharmaceutical market. By Friedel-Crafts acylation reaction, the C-3 of this ring makes it susceptible to chemically react. Thus, 12 indole analogues were synthesized as potential larvicidal agents against Ae. aegypti in its 3rd larval stage followed by the evaluation of the toxicity in nauplii of Artemia sp. The compounds were identified by analytical thin-layer chromatography, purified on a silica gel 60 chromatographic column (using the Hexane: Ethyl acetate (90:10, v / v) binary system as the mobile phase) and characterized by melting point, 13C and 1H NMR (using a residual solvent peak or TMS as reference for 1 H NMR spectra), mass spectrum and infrared. Bioassays were performed using 20 larvae per test, disposable cups containing 20mL of the test solution in triplicate. Branched aliphatic side chain derivatives were more potent than the others were, and the linear ones exhibited potency oscillation as the addition of the methylene chains. Toxicity tests indicated that (3-chlorophenyl)1-(1H-indol-3-yl)methanone, with moderate larvicidal potency (LC50 = 50.59 ppm), showed the highest selectivity index (SI >19.7), being less toxic to Artemia sp. than Ae. aegypti. The relationships between structural changes in indole derivatives and their LC50 results provide information that may contribute to the understanding of the influence of physicochemical properties on the larvicidal action of this class of compounds, without damage to the ecosystem.
Consideradas doenças virais de grande reemergência no mundo, a dengue, chikungunya e zika têm como principal vetor o Aedes aegypti (L.) (Diptera:Culicidae). O controle vetorial baseado em larvicidas é uma medida importante de prevenir a transmissão de tais infecções. Um grande desafio nas formas de controle desse artrópode está na baixa sensibilidade de sua população através do uso de larvicidas convencionais. Assim, o inseto adquire resistência, reduzindo a eficácia desses pesticidas. Consequentemente, levará a um aumento nos riscos de toxicidade em organismos não-alvo e uma alteração ao meio ambiente. Uma alternativa para evitar problemas ocasionados pela utilização desses produtos é a pesquisa por novos compostos com menos impacto ambiental e melhores benefícios à saúde humana. O anel da molécula do indol representa uma das subunidades com grande importância na descoberta de novos produtos pesticidas para o mercado farmacêutico. Por reação de acilação de Friedel-Crafts, o C-3 deste anel torna suscetível a reagir quimicamente. Assim, foram sintetizados 12 análogos do indol como potenciais agentes larvicidas contra o Ae. aegypti no seu 3º estágio larvar, seguido pela avaliação da toxicidade em náuplios de Artemia sp. Os compostos sintetizados foram identificados por cromatografia em camada delgada analítica, purificados em coluna cromatográfica de sílica gel 60 (utilizando o sistema binário Hexano: Acetato de etila (90:10, v/v) como fase móvel), e caracterizados por ponto de fusão, RMN de 13C e 1H (utilizando um pico de solvente residual ou TMS como referência para os espectros de RMN 1H), espectro de massas e infravermelho. Os bioensaios foram realizados utilizando 20 larvas por teste, copos descartáveis contendo 20 mL da solução teste em triplicata. Derivados de cadeias laterais alifáticas ramificadas foram mais potentes que os demais, e os lineares exibiram oscilação de potência conforme acréscimo das cadeias de metileno. Ensaios de toxicidade apontaram que a (3-clorofenila)1-(1H-indol-3- ila)metanona, com potência larvicida moderada (CL50 = 50,59 ppm), exibiu o maior índice de seletividade (IS >19,7), sendo menos tóxico para Artemia sp do que para o Ae. aegypti. As relações entre mudanças estruturais dos derivados do indol e seus resultados de CL50 fornecem informações que podem contribuir para a compreensão da influência de propriedades físico-químicas na ação larvicida desta classe de compostos, sem prejuízos ao ecossistema.
São Cristóvão, SE
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Figueiredo, Pablo Busatto. "Estudo quÃmico e atividade larvicida do Ãleo essencial das folhas de Piper aduncum L." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=12023.
Анотація:
CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel SuperiorOs Ãleos essenciais produzidos por diversas espÃcies vegetais sÃo misturas, por vezes, bastante complexas com variadas funÃÃes ecolÃgicas como inibiÃÃo de germinaÃÃo, proteÃÃo contra predadores, atraÃÃo de polinizadores, proteÃÃo contra perda de Ãgua e aumento da temperatura. Os Ãleos essenciais sÃo obtidos atravÃs de mÃtodos fÃsicos de extraÃÃo e apresentam diversas aplicaÃÃes, como flavorizantes, conservantes, fixadores, agentes antibacterianos e antifÃngicos. VÃrios produtos industriais tambÃm utilizam Ãleos essenciais em suas formulaÃÃes, como pesticidas, repelentes e atraentes se apresentando como uma alternativa natural no controle ou na eliminaÃÃo de pragas. A restriÃÃo de alguns pesticidas sintÃticos promoveu ainda mais a pesquisa em busca de novos produtos que pudessem desempenhar a funÃÃo daqueles que nÃo estÃo mais disponÃveis no mercado. VÃrios biopesticidas estÃo relatados na literatura e dentre eles o dilapiol, que à um fenilpropanoide de atividade inseticida comprovada, encontrado em alto teor no Ãleo essencial de Piper aduncum L. (Piperaceae). Neste trabalho, foi analisada a variabilidade quantitativa do dilapiol nos Ãleos essenciais (OE) obtidos das folhas frescas de P. aduncum e quando submetidas a 24, 48, 72 e 96h de secagem a 40 ÂC. Os OE foram submetidos a reaÃÃes de hidrogenaÃÃo e hidroxilaÃÃo em busca de biopesticidas mais ativos e todos os produtos foram analisados por CG-EM, RMN1H e RMN13C. O teor de dilapiol foi analisado por CGâFID e por CLAE, obtendo-se em mÃdia 89,68% e 89,91% respectivamente, e permitiu concluir que a temperatura de secagem nÃo influencia no conteÃdo deste composto presente no OE de P. aduncum. O segundo componente majoritÃrio do Ãleo foi identificado como β-cariofileno (4,47%). O ensaio larvicida frente a Aedes aegypti realizado forneceu IC50 de 47,12 mg/L, confirmando o potencial deste produto como biopesticida.
Essential oils produced by various plant species are mixtures sometimes quite complex with varied ecological functions, such as inhibition of germination, protection against predators, attraction of pollinators, protection against water loss and temperature rise. Essential oils are obtained by physical extraction methods and present several applications, such as flavorings, preservatives, fixatives, antibacterial and antifungal agents. Various industrial products also use essential oils in their formulations, such as pesticide repellents and attractive presenting as a natural alternative to control or eliminate pests. The restriction of some synthetic pesticides further promoted the search for new products that could play the role of those who are no longer available in the market. Several biopesticides are reported and among them dilapiolle, which is a phenylpropanoid with proven insecticidal activity found in high content in essential oil of Piper aduncum L. (Piperaceae). In this work, we analyzed the quantitative variability in dilapiolle essential oils (EO) obtained from fresh leaves of P. aduncum and when subjected to 24, 48, 72 and 96 h of drying at 40 ÂC. The EO underwent hydrogenation and hydroxylation reactions in search of more active biopesticides and all products were analyzed by GC-MS, NMR 1H and NMR 13C. The content of dilapiolle was analyzed by GC-FID and HPLC, yielding on average 89.68 % and 89.91 % respectively and showed that the drying temperature does not influence the content of this compound in P. aduncum EO. The second major component of the oil was identified as β-caryophyllene (4.47 %). The test larvicide against Aedes aegypti performed provided IC50 of 47.12 mg/L, confirming the potential of this product as a biopesticide.
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Santos, Daniela Braz dos. "Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae)." Universidade Federal de Goiás, 2015. http://repositorio.bc.ufg.br/tede/handle/tede/5759.
Анотація:
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The main transmitter species of serotypes of the dengue virus is Aedes aegypti, the most competent mosquitoes and more adapted to urban and man-made environment. The control measures of this disease are still mainly on the vector. Recently, several studies have shown the use of botanical origin of substances with potential insecticide in control of Ae. aegypti, alternatively opposite resistance to conventional insecticides. The efficacy oleoresin of C. langsdorffii the control of Ae. aegypti demonstrated in field work, signals the promising possibility of this botanical compound to control this vector requiring studies to clarify the mechanism of action. Thus, it is proposed to establish the mechanism of death process in the third stage larvae of Ae. aegypti through morfohistologic studies. The larvae of Ae. aegypti were subjected to oil-resin solutions and hexane fraction C. langsdorffii in concentrations of 70 ppm and 80 ppm, respectively. Subsequently collected in time intervals of 6h, 12h and 24h. They were fixed in 4% paraformaldhyde in 0.1 M sodium phosphate buffer pH 7.2, embedded in resin, mounted, stained with hematoxylin-eosin and analyzed by light microscopy. The oil-resin and hexane fraction C. langsdorffii, caused the death of the larvae by the destruction of the posterior cells of the midgut region through cytoplasmatic vacuolation, apical cytoplasmic vesicle formation, lip brush degeneration, increased cellular volume and fold in peritrophic matrix. The changes described in this paper demonstrate how bioactive compounds can trigger cell degeneration and accelerate cell disruption.
A principal espécie transmissora dos sorotipos do vírus dengue é o Aedes aegypti, o mosquito mais competente e mais adaptado ao ambiente urbano e antrópico. As medidas de controle dessa doença ainda estão prioritariamente sobre o vetor. Recentemente, diversos estudos têm evidenciado o uso de substâncias de origem botânica com potencial inseticida no controle de Ae. aegypti, como alternativa frente à resistência aos inseticidas convencionais. A eficácia do óleo-resina de Copaifera langsdorffii no controle das larvas de Ae. aegypti demonstrada em trabalhos de campo, sinaliza a possibilidade promissora desse composto botânico para o controle desse vetor requerendo estudos que esclareçam o seu mecanismo de ação. Dessa forma, propõe-se demonstrar o mecanismo do processo de mortalidade em larvas de terceiro estádio de Ae. aegypti, através de estudos morfohistológicos. As larvas de Ae. aegypti foram submetidas às soluções de óleo-resina e fração hexânica de C. langsdorffii nas concentrações de 70 ppm e 80 ppm, respectivamente. Posteriormente coletadas nos intervalos de tempo de 6 horas, 12horas e 24horas. Foram fixadas em paraformaldeído a 4% com tampão fosfato de sódio 0,1 M pH 7,2, incluídas em resina, montadas, coradas pela hematoxilina-eosina e analisadas por microscopia de luz. O óleo-resina e fração hexânica de C. langsdorffii, causaram a morte das larvas pela destruição das células da região posterior do mesêntero, por meio de intensa vacuolização citoplasmática, formação de vesícula citoplasmática apical, degeneração do bordo em escova, aumento do volume celular e a dobra na matriz peritrófica. As alterações descritas neste trabalho demonstram como compostos bioativos desencadeiam a degeneração celular e aceleram o rompimento celular.
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Carvalho, Jarbas Lima de. "Chemical and biological study of Bauhinia pulchella Benth." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13413.
Анотація:
Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicoThis present work reports the chemical and biological analysis of the stem and leaves from Bauhinia pulchella. In this study, the ethanol extract from stems was obtained by maceration, subjected to chromatographic fractionation, leading to isolation of three flavonoids: (+)-3â,4â-dihydroxyphenyl-chroman-7-ol (BP-2), (-)-fisetinidol (BP-3) and (+)-epicatechin (BP-4); a mixture of triterpenes taraxerone and β-amyrenone (BP-1); a mixture of steroids sitosterol and stigmasterol (BP-5); and a bibenzyl named 2-hydroxy-3â,5â-dimethoxybibenzyl (BP-6). It is notewhorthy to mention that BP-1 and BP-4 substances are unprecedented in the genus, while BP-2 is unpublished. Chemical structures of secondary metabolites obtained were elucidated by 1H and 13C NMR; IR and MS associated with comparison of data described in the literature. Chemical composition of the essential oil from leaves of B. pulchella, obtained by hydrodistillation, was determined and quantified by gas chromatography-mass spectroscopy (GC/MS) and gas chromatography-flame ionization detector (GC/FID), which identified 95,68% of all constituents. α-pinene (23.89%); caryophyllene oxide (22.43%) and β-pinene (12.19%) were the major components. The essential oil was tested against Aedes aegypti larvae and showed LC50 value of 105.93 Â 1.48 μg/mL. The cytotoxic activity of essential oil was evaluated on human tumor cell lines (HL-60; MCF-7; NCI-H292 and HEP-2) was evaluated, showing IC50 values with confidence intervals of 9.941 (8.238 to 12.00), 53.05 (41.39 to 67.99), 48.98 (44.22 to 54.25) and 50.42 (42.47 to 59.87) μg/mL, respectively and the cell line HL-60 the most sensitive among the cells tested. This is the first report of the chemical study of Bauhinia pulchella, as well the investigation of larvicidal activity and cytotoxicity of the essential oil from its leaves.
O presente trabalho relata o estudo quÃmico e biolÃgico do caule e das folhas de Bauhinia pulchella. Nesse estudo, o extrato etanÃlico do caule, obtido por maceraÃÃo, foi submetido a fracionamento cromatogrÃfico levando ao isolamento de trÃs flavonoides (+)-3â-4âdiidroxifenil-cromano-7-ol (BP-2), (-)-fisetinidol (BP-3) e (+)-epicatequina (BP-4); da mistura de triterpenos taraxerona e β-amirenona (BP-1); da mistura de esteroides sitosterol e estigmasterol (BP-5) e de um bibenzil denominado 2-hidrÃxi-3â-5â-dimetoxibibenzila (BP-6). Cabe ressaltar que as substÃncias BP-1 e BP-4 sÃo inÃditas no gÃnero, enquanto BP-2 à inÃdita na literatura. As estruturas dos metabÃlitos secundÃrios isolados foram elucidadas por RMN 1H e 13C; IV e EM, juntamente com a comparaÃÃo com os dados descritos na literatura. A composiÃÃo quÃmica do Ãleo essencial das folhas de B. pulchella, obtido por hidrodestilaÃÃo, foi determinada e quantificada por cromatografia gasosa acoplada à espectrometria de massas (CG-EM) e detector de ionizaÃÃo por chama (CG-DIC), sendo, portanto, identificados 95,68% dos seus constituintes: α-pineno (23,89%), Ãxido de cariofileno (22,43%) e β-pineno (12,19%) foram os constituintes majoritÃrios. O Ãleo essencial teve sua atividade larvicida sobre Aedes aegypti avaliada, sendo obtido um valor de CL50 igual a 105,93  1,48 μg/mL. O poder citotÃxico do Ãleo essencial foi avaliado sobre as linhagens tumorais humanas HL-60, MCF-7, NCI-H292 e HEP-2, sendo obtidos valores de CI50 e intervalos de confianÃa iguais a 9,941 (8,238 a 12,00); 53,05 (41,39 a 67,99); 48,98 (44,22 a 54,25) e 50,42 (42,47 a 59,87) μg/mL, respectivamente, sendo a linhagem celular HL-60 a mais sensÃvel dentre as cÃlulas testadas. Este à o primeiro relato do estudo quÃmico de Bauhinia pulchella, bem como da investigaÃÃo da atividade larvicida e citotÃxica do Ãleo essencial de suas folhas.
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Sousa, Manaces Cunha. "Estudos químicos e avaliação antioxidante, bactericida e larvicida do óleo essencial do Ocimum basilicum L (ALFAVACA)." Universidade Federal da Paraíba, 2010. http://tede.biblioteca.ufpb.br:8080/handle/tede/7170.
Анотація:
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Previous issue date: 2010-02-11Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
This study aimed to investigate major metabolites from the aerial parts of Ocimum basilicum by a phytochemical screening. Quantitatively extracting the essential oil and subjecting it to thermal analysis and chromatography, as well as verifying its performance as an antioxidant, antibacterial and larvicidal agents. In this study it was observed that the aerial parts of Ocimum basilicum present tannins, depsides and depsidons, free steroids, flavonoids, saponins, flavonoid aglicons, triterpenoid aglicons, steroids and organic acids. The yield of essential oil extracted varied between 1.5 and 2.0% for the dried aerial parts, while the one of fresh aerial parts was between 0.28 to 1.0%. The study of essential oil plant species by gas chromatography coupled with mass spectrometry, revealed that the essence of it consists of six components, metilchavicol, linalool, eucaliptoll and farnesene, the main metabolites. The thermal study of the essential oil made by termogravimetry in air atmosphere and nitrogen, showed a similar thermal profile, indicating two mass loss attributed to volatilization of terpenes fraction and decomposition of aromatic fraction respectively. Antioxidant, antibacterial and larvicidal analysis revealed that the essential oil from dried aerial parts of Ocimum basilicum has outperformed the essential oil of fresh aerial parts when tested under the same conditions.
Este trabalho teve como objetivo estudar os principais metabólitos das partes aéreas do Ocimum basilicum através de uma triagem fitoquímica. Extrair quantitativamente seu óleo essencial e submetê-lo a análises térmicas e cromatográficas, assim como verificar seu desempenho como agente antioxidante, antibacteriano e larvicida. Neste trabalho, foi observado que as partes aéreas do Ocimum basilicum apresentam taninos, depsídeos e depsidonas, esteroides livres, flavonoides, saponinas, aglicona flavonóides, aglicona triterpenóides, esteróides e ácidos orgânicos. O rendimento do óleo essencial extraído variou entre 1,5 a 2,0% para as partes aéreas secas, enquanto o rendimento das partes aéreas frescas ficou entre 0,28 a 1,0%. O estudo do óleo essencial da espécie vegetal por cromatografia em fase gasosa acoplada a espectrometria de massas revelou que a essência do mesmo é formada por seis componentes, sendo o metilchavicol, linalol, eucaliptoll e farneseno os principais metabólitos. O estudo térmico do óleo essencial realizado por TG em atmosfera de ar e nitrogênio revelou um perfil térmico semelhante, Indicando duas perdas de massa atribuídas a volatilização da fração terpênica e decomposição da fração aromática respectivamente. As análises antioxidantes, antibacterianas e larvicidas revelaram que o óleo essencial das partes aéreas secas do Ocimum basilicum apresenta desempenho superior ao óleo essencial das partes aéreas frescas, quando testado nas mesmas condições de análises.
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Soares, Coelho Juliene. "Atividade hemaglutinante e larvicida (Aedes aegypti) na água tratada com sementes de Moringa oleifera." Universidade Federal de Pernambuco, 2007. https://repositorio.ufpe.br/handle/123456789/1847.
Анотація:
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Previous issue date: 2007Sementes de Moringa oleifera têm sido freqüentemente usadas como coagulante para o tratamento de água no Nordeste do Brasil, local com alta incidência de dengue. Lectinas constituem um grupo de proteínas heterogêneas capazes de reconhecer e ligar reversivelmente carboidratos e glicoproteínas. Inibidores de protease impedem a atividade enzimática pela formação de complexos estáveis com tripsina e/ou quimotripsina. Lectinas e inibidores de tripsina com atividade inseticida têm sido descritos. O objetivo deste trabalho foi avaliar na água tratada com sementes de M. oleifera, a partir das atividades hemaglutinante (AH), inibidora de tripsina e larvicida. Água destilada foi tratada com 1, 3, 6 ou 15 sementes de M. oleifera (MoW). Ensaio para lectina usou eritrócitos de coelho. MoW foi avaliada para atividade inibidora de tripsina usando N-α-benzoil-DL-arginil-ρ-nitroanilida (BAPNA). Bioensaio larvicida foi feito usando larvas do vetor da dengue, Aedes aegypti. Desenvolvimento, mortalidade e aspectos morfológicos das larvas foram analisados. MoW contém AH e não foi capaz de inibir tripsina. Redução significante do desenvolvimento larval foi observado após 24, 48 e 72 h de incubação com MoW. Larvas no 4º estádio (L4) foram detectadas somente no controle, MoW1 e MoW3. Significante (p<0. 0001) taxa de mortalidade foi detectada na preparação lectínica mais ativa, MoW15 (45%). Diferenças morfológicas nas larvas em L4 incubadas com MoW1 foram observadas quando comparadas ao controle por microscopia ótica invertida. Os resultados obtidos sugerem a avaliação das sementes de M. oleifera para o controle do vetor da dengue. A presença de AH na preparação ativa sobre as larvas do A. aegypti pode indicar o envolvimento de lectina no efeito larvicida de MoW
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Cruz, Pedro Ernesto Oliveira da. "Estudo fitoquímico e investigação das atividades antioxidante, antimicrobiana e larvicida das cascas de Annona salzmannii A. DC. (Annonaceae)." Universidade Federal de Sergipe, 2011. https://ri.ufs.br/handle/riufs/6160.
Анотація:
The presente work describes the results obtained from the phytochemical studies bioguided of crude extracts of the bark of A. salzmannii, a species belonging to the Annonaceae family, front to tests of antioxidant, antimicrobial, and insecticide activities against larvae of the mosquito Aedes aegypti. From the hexane extract were isolated five compounds, been three sesquiterpenes (caryophyllene oxide,
spathulenol, and selin-11-en-4a-ol); and two steroids (stigmast-4-en-3-one and - sitosterol). From the chloroform alkaloidal fraction of the methanolic extract were isolated seven alkaloids: liriodenine, 10-methoxyliriodenine, anonaine, asimilobine, reticuline, cleistopholine, and a mixture of alkaloids anonaine and xylopine. The assay of antioxidant activity revealed that the methanol extract (3583.02 μmol of
TE g-1) and its fractions, chloroform alkaloidal fraction (5291.77 μmol of TE g-1) and chloroform neutral fraction (2047.54 μmol of TE g-1) showed significant antioxidant
activity, mainly the chloroform alkaloidal fraction. Among the alkaloids isolated and tested, asimilobine was the most active of them with antioxidant activity of 2.09 TE relative. For antimicrobial assay, the best results were observed for methanol extract and chloroform alkaloidal fraction against Staphylococcus aureus (ATCC14458), Escherichia coli (ATCC 10799), Candida tropicalis (ATCC 157), and C. dubliniensis (ATCC 777). Among the alkaloids isolated and tested, all showed significant results with MIC values between 25-100 mg.mL-1, mainly the alkaloids anonaine, asimilobine, and liriodenine against Staphylococcus aureus penicilinase-
(8-), S. epidermidis (ATCC 12228), S. epidermidis (6ep), and Candida dubliniensis (ATCC 778157). To the test of larvicidal activity, only the hexane extract and the fractions of methanol extract showed activity below 700 mg.mL-1. The caryophyllene oxide showed activity of 167 mg.mL-1. The results obtained confirm that A. salzmannii is a typical species of the Annonaceae family, and a promising source of
biologically active compounds.O presente trabalho descreve os resultados obtidos a partir do estudo fitoquímico biomonitorado dos extratos brutos das cascas de Annona salzmannii, uma espécie pertencente a família Annonaceae, frente a testes de atividades antioxidante, antimicrobiana e larvicida contra o Aedes aegypti. Do extrato hexânico foram isoladas cinco substâncias, sendo três sesquiterpenos (óxido de cariofileno, espatulenol e selin-11-en-4a-ol); e dois esteróides (estigmast-4-en-3-ona e - sitosterol). Da fração clorofórmica alcalóidica, proveninente do extrato metanólico, isolou-se sete alcalóides: liriodenina, 10-metoxiliriodenina, anonaina, asimilobina, reticulina, cleistofolina e uma mistura dos alcalóides anonaina e xylopina. O ensaio de atividade antioxidante revelou que o extrato metanólico (3583,02 μmol de TE g-1) e suas respectivas frações, fração clorofórmica alcalóidica (5291,77 μmol de TE g-1) e fração clorofórmica neutra (2047,54 μmol de TE g-1) apresentaram expressiva atividade antioxidante, com destaque para a fração clorofórmica alcaloídica. Dentre os alcalóides isolados e testados, asimilobina foi o mais ativo com atividade antioxidante de 2,09 TE relativo. Para o ensaio de atividade antimicrobiana, os melhores resultados foram observados para o extrato metanólico e a fração clorofórmica alcaloídica contra Staphylococcus aureus (ATCC14458), Escherichia coli (ATCC 10799), Candida tropicalis (ATCC 157) e C. dubliniensis (ATCC 777). Dentre os alcalóides isolados e testados, todos apresentaram resultados significativos com valores de CIM entre 25-100 mg.mL-1, com destaque para os alcalóides anonaina, asimilobina e liriodenina frente a Staphylococcus aureus penicilinase-(8-), S. epidermidis (ATCC 12228), S. epidermidis (6ep) e Candida dubliniensis (ATCC 778157). Para o teste de atividade larvicida, apenas o extrato hexânico e as frações provenientes do extrato metanólico apresentaram atividade abaixo de 700 mg.mL-1. O óxido de cariofileno apresentou atividade de 167 mg.mL-1. Os resultados obtidos confirmam que A. salzmannii é quimicamente uma espécie típica da família Annonaceae, além de uma fonte promissora de substâncias biologicamente ativas.
Частини книг з теми "Activité larvicide":
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DeMilo, Albert B., Stephen B. Haught, and Hugh D. Sisler. "Larvicidal and Fungicidal Activity of Compounds with Hydrazinecarboxamide and Diazenecarboxamide Moieties." In ACS Symposium Series, 273–86. Washington, DC: American Chemical Society, 1987. http://dx.doi.org/10.1021/bk-1987-0355.ch025.
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Tarmadi, Didi, Dwi Jayanti Gunandini, and Sulaeman Yusuf. "Larvicidal Activity of Cerbera odollam Gaertn Against a Dengue Vector, Aedes aegypti (Diptera: Culicidae)." In Sustainable Future for Human Security, 175–88. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5430-3_14.
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Tine-Djebbar, Fouzia, Djemaa Dris, Radja Guenez, Samir Tine, and Noureddine Soltani. "Larvicidal Activity of Lamiaceae and Lauraceae Essential Oils and Their Effects on Enzyme Activities of Culex pipiens L. (Diptera: Culicidae)." In Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition), 709–16. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-51210-1_112.
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Deepika, Subramanyam, Selvaraj Mohana Roopan, and Chinnadurai Immanuel Selvaraj. "Bionanocomposite assembly with larvicidal activity against Aedes aegypti." In Applications of Nanobiotechnology for Neglected Tropical Diseases, 401–25. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-821100-7.00001-7.
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"Mosquito Larvicidal Activity of Marine Plant Extracts with Synthetic Insecticides." In Volume 34, 1991, 537–40. De Gruyter, 1991. http://dx.doi.org/10.1515/9783112328101-068.
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"- Biosynthesis and Characterization of Different Nanoparticles and Its Larvicidal Activity against Human Disease Vectors." In Marine Biomaterials, 296–311. CRC Press, 2013. http://dx.doi.org/10.1201/b14723-18.
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"4. Ecological Impact of Human Activity using Medium and Local-Scale Assessments." In Trente ans de lutte contre l’onchocercose en Afrique de l’Ouest. Traitements larvicides et protection de l’environnement, 21–30. IRD Éditions, 2003. http://dx.doi.org/10.4000/books.irdeditions.28725.
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"3. Ecotoxicological Impact of Human Activity at the Basin Scale: Assessment of Chemical Loads." In Trente ans de lutte contre l’onchocercose en Afrique de l’Ouest. Traitements larvicides et protection de l’environnement, 13–19. IRD Éditions, 2003. http://dx.doi.org/10.4000/books.irdeditions.28722.
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Romano, Camila Aline, Matheus Gabriel de Oliveira, Matheus de Sousa Melo Morais, Andressa Tuane de Santana Paz, Liliane de Sousa Silva, Heloisa Helena Garcia da Silva, Ionizete Garcia da Silva, Adelair Helena dos Santos, and José Realino de Paula. "CHEMICAL CONSTITUENTS AND LARVICIDAL ACTIVITY OF ESSENTIAL OIL FROM Murraya koenigii (L.) Spreng. (RUTACEAE) AGAINST Aedes aegypti (DIPTERA: CULICIDAE)." In Avanços das Pesquisas e Inovações na Engenharia Química 2, 181–91. Atena Editora, 2020. http://dx.doi.org/10.22533/at.ed.51520240318.
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Antwi, L. A. K. "Effects of Aerial Spraying of Chlorphoxim on the Brain Acetylcholinesterase Activity of Fish from Three Rivers in the Ivory Coast, West Africa." In Trente ans de lutte contre l’onchocercose en Afrique de l’Ouest. Traitements larvicides et protection de l’environnement. IRD Éditions, 2003. http://dx.doi.org/10.4000/books.irdeditions.28668.
Тези доповідей конференцій з теми "Activité larvicide":
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Silva-Alves, Diana C. B. da, Janaína V. dos Anjos, Nery N. M. Cavalcante, Daniela M. do A. F. Navarro, Geanne K. N. Santos, and Rajendra M. Srivastava. "Synthesis of new isoxazole derivatives with larvicidal activity." In 14th Brazilian Meeting on Organic Synthesis. São Paulo: Editora Edgard Blücher, 2013. http://dx.doi.org/10.5151/chempro-14bmos-r0284-1.
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"Larvicidal Activity of Mistletoe Lectin on Lepidopteran Pests: Mechanisms of Action." In International Conference on Civil, Biological and Environmental Engineering. International Institute of Chemical, Biological & Environmental Engineering, 2014. http://dx.doi.org/10.15242/iicbe.c514525.
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Santhanalakshmi, K., Gomathi Thandapani, K. Margandan, and N. Neelakandeswari. "Larvicidal activity of 1,3,4- oxadiazole analogues and their Molecular Docking Studies." In 2022 International Conference on Smart Technologies and Systems for Next Generation Computing (ICSTSN). IEEE, 2022. http://dx.doi.org/10.1109/icstsn53084.2022.9761366.
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Santhanalakshmi, K., Gomathi Thandapani, K. Margandan, and N. Neelakandeswari. "Larvicidal activity of 1,3,4- oxadiazole analogues and their Molecular Docking Studies." In 2022 International Conference on Smart Technologies and Systems for Next Generation Computing (ICSTSN). IEEE, 2022. http://dx.doi.org/10.1109/icstsn53084.2022.9761366.
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Castillo-Garit, Juan, Yudith Cañizares-Carmenate, Mirelys Hernandez-Morfa, Gloria Castellano, and Francisco Torrens. "Multiple Linear Regression to predict larvicidal activity against Aedes aegypti mosquito." In MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd edition. Basel, Switzerland: MDPI, 2017. http://dx.doi.org/10.3390/mol2net-03-04628.
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Carvalho, Mariana T., Gisele S. de Santana, Audrey N. de Andrade, Amanda Cabral, Maria Alice V. de Melo-Santos, Anderson S. L. Gomes, and Nairane S. Rosa-Leão. "Exploiting silver nanoparticles with PMAA against aedes aegypti larvae development: potential larvicidal activity." In Colloidal Nanoparticles for Biomedical Applications XIII, edited by Xing-Jie Liang, Wolfgang J. Parak, and Marek Osiński. SPIE, 2018. http://dx.doi.org/10.1117/12.2291573.
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Lé, Nayana, Fabiola Nunes, and Kristerson Freire. "Study of the larvicide activity and toxicity of <em>Humulus lupulus</em> extract and beer hop residue , part 1: larvicide activity in <em>Aedes aegypti</em>." In MOL2NET'21, Conference on Molecular, Biomedical & Computational Sciences and Engineering, 7th ed. Basel, Switzerland: MDPI, 2021. http://dx.doi.org/10.3390/mol2net-07-11654.
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Rahayu, Sofia Ery, Sulisetijono, Nur Qomariyah, and Filda Sherly Rohmani. "Larvicidal activity of Carica pubescens leaf and petiole extract against Aedes aegypti L. larvae." In INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020). AIP Publishing, 2021. http://dx.doi.org/10.1063/5.0053084.
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Barros, Maria Ester de Sá B., Juliano C. R. Freitas, Daniela M. A. F. Navarro та Paulo Henrique Menezes. "Synthesis and Larvicidal Activity of of α,β-Unsaturaded δ- Lactones against Aedes aegypti". У 14th Brazilian Meeting on Organic Synthesis. São Paulo: Editora Edgard Blücher, 2013. http://dx.doi.org/10.5151/chempro-14bmos-r0216-1.
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"Mosquito Larvicidal Activity of Eucalyptus deglupta Crude Extract and Analysis of its Bioactive Compounds." In Multi-Disciplinary Manila (Philippines) Conferences Jan. 23-24, 2017, Manila (Philippines). Universal Researchers (UAE), 2017. http://dx.doi.org/10.17758/uruae.ae0117604.