Дисертації з теми "Acelerated Bone fracture healing"
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "Acelerated Bone fracture healing".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
Richardson, James Bruce. "The mechanics of fracture healing." Thesis, University of Aberdeen, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.290866.
Повний текст джерелаLi, Jiang, and 李江. "Bone fracture healing in laminopathy-based premature aging." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45142233.
Повний текст джерелаYew, Alvin Garwai. "The equilibrium geometry theory for bone fracture healing." College Park, Md. : University of Maryland, 2008. http://hdl.handle.net/1903/8308.
Повний текст джерелаThesis research directed by: Dept. of Mechanical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
Mawhinney, Ian Nicholas. "Bone and ultrasound." Thesis, Queen's University Belfast, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.335942.
Повний текст джерелаManitzky, Louise J. "Mathematical modelling of intramembranous bone formation during fracture healing." Thesis, Queensland University of Technology, 2014. https://eprints.qut.edu.au/78983/1/Louise_Manitzky_Thesis.pdf.
Повний текст джерелаMahmud, Fares A. "The electromechanical properties of bone." Thesis, Staffordshire University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.254325.
Повний текст джерелаWatkins, P. E. "A study of mechanical influences on fracture healing, and on fracture non-union." Thesis, University of Bristol, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376622.
Повний текст джерелаMurray, Alastair W. "Fracture healing in osteopenic bone and the influence of simvastatin." Thesis, University of Edinburgh, 2007. http://hdl.handle.net/1842/29286.
Повний текст джерелаKaluarachchi, Thambilipitiyage Kusumsiri Priyantha Kumara. "Impact of collagen type X deficiency on bone fracture healing." Thesis, Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23501807.
Повний текст джерелаNyquist, Fredrik. "The influence of alcohol on bone metabolism and fracture healing." Lund : Lund University, Dept. of Orthopaedics, Malmö University Hospital, 1998. http://catalog.hathitrust.org/api/volumes/oclc/39792795.html.
Повний текст джерелаSchlundt, Claudia. "Impact of the adaptive immune system in bone fracture healing." Doctoral thesis, Humboldt-Universität zu Berlin, 2017. http://dx.doi.org/10.18452/18195.
Повний текст джерелаBone tissue possesses the remarkable capacity to fully regenerate after injury. However, in 10-15% of patients, unsuccessful bone repair is still a present problem. Components of the adaptive immune system play an indispensable role in bone regeneration. The here presented PhD thesis focused on the interaction of CD4+ regulatory T cells (Treg) during fracture healing. In a murine osteotomy model, the spatiotemporal distribution of immune and bone cells was analyzed within the healing bone. Cells of the immune system were detectable throughout the whole healing cascade in the injured area und showed often a direct co-localization with bone cells. These results highlight the interconnectivity of immune and bone cells during regeneration. By adoptive transfer of murine CD4+ Treg prior to osteotomy, an immunomodulatory approach to improve bone healing was conducted. Mice possessing an unexperienced immune system (SPF housing) showed a consistent improved healing outcome after adoptive Treg transfer. However, mice with a more experienced immune system (semi-sterile housing) receiving an adoptive Treg transfer demonstrated a controversial healing outcome: half of the mice showed a significantly improved and the other half a significantly poorer healing outcome. In the mice with a poorer healing outcome, a higher ratio of CD8+ effector T cells and Treg was observed. In a following proof of concept study, a pre-osteotomy defined ratio of CD8+ effector T cells and Treg could predict the healing outcome after adoptive Treg transfer and osteotomy. A potential positive impact of Treg in bone repair was confirmed in this study. However, the tremendous impact of the environment and thereby of the immune status prior to immunomo-dulation was also clearly demonstrated. Hence, for the clinic, it is even more important to develop and to apply patient based immunomodulatory treatment approaches considering the individual immune status of each patient prior to treatment.
Chehade, Mellick Joseph. "Bone resonance analysis, histomorphometry and the mechanics of fracture healing." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phc515.pdf.
Повний текст джерелаSchütte, Andrea. "Autoantibodies against growth factors and their receptors in fracture healing." Doctoral thesis, Humboldt-Universität zu Berlin, Lebenswissenschaftliche Fakultät, 2016. http://dx.doi.org/10.18452/17656.
Повний текст джерелаRegeneration of bone during fracture healing includes concerted actions of growth factors. Some fractures show delayed healing or non-union due to as yet unknown reasons. Neutralizing autoantibodies (aAB) against growth factors or their receptors might influence and potentially impair the bone healing capacity. In this study, a cohort of 265 fracture patients with different treatment regimen and healing outcomes were analysed. Autoantibodies against IGF1 receptor, insulin receptor, BMP7, BMP2, IGF1 and (pro)insulin were measured in sera of these fracture patients. The prevalence of aAB against IGF1R and IR was 5% and 6% in fracture patients, respectively. The appearance of IGF1R- and IR-aAB was not induced by the surgical intervention and was unrelated to the healing outcome. BMP7-aAB were found in 1-2.5% of healthy subjects and in fracture patients that were not treated with rhBMP7. Patients that had received rhBMP7 treatment showed a higher incidence of BMP7-aAB positivity of 6% at surgery and 18% four weeks post surgery. BMP2-aAB were found in 2% of healthy controls and 6% of the fracture patients that were treated with rhBMP7. Characterizing the biological effect of BMP7-aAB in a cell-based reporter assay, a neutralizing effect was observed for samples with high titres. As the most relevant clinical outcome, the criterion consolidation was analysed defining whether the fracture gap was closed after six months or not. The presence of BMP-aAB was not significantly associated with the healing outcome. In summary, novel diagnostic assays for the detection and quantification of growth factor and receptor aAB were generated and used to determine aAB in sera from fracture patients. None of the identified aAB were negatively associated with the regeneration process or healing outcome. Ongoing concerns regarding the safety of rhBMP7 treatment are justified as the biological treatment induces aAB against BMP7 that block the BMP signal transduction.
Krishnakanth, Pushpanjali. "Mechanical considerations in fracture fixation." Thesis, Queensland University of Technology, 2012. https://eprints.qut.edu.au/51002/1/Pushpanjali_Krishnakanth_Thesis.pdf.
Повний текст джерелаOess, Ninja P. "Strain measurements using magnetoelastic sensing for wireless bone fracture healing assessment /." Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17550.
Повний текст джерелаBartnikowski, Nicole J. "Modifying fixation stiffness to improve bone healing." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/97997/1/Nicole_Bartnikowski_Thesis.pdf.
Повний текст джерелаLöffler, Julia [Verfasser]. "Impact of the local metabolic milieu on bone fracture healing / Julia Löffler." Berlin : Freie Universität Berlin, 2019. http://d-nb.info/1192304195/34.
Повний текст джерелаScarpa, Edoardo. "Polymeric nanoparticles loaded with a Wnt agonist for enhancing bone fracture healing." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/408720/.
Повний текст джерелаRohrbach, Daniel. "Quantitative ultrasound in transverse transmission for bone quality assessment and monitoring fracture healing." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16813.
Повний текст джерелаOsteoporosis and impaired bone healing are of high relevance. A promising non-invasive, non-ionizing candidate for fracture risk prediction and monitoring fracture healing is quantitative ultrasound (QUS). However, the acceptance of QUS for bone quality assessment is still not comparable to X-ray based methods. Scanning acoustic microscopy (SAM) and Synchrotron Radiation micro-computer tomography (SRµCT) has been used to investigate anatomical and age dependent variations of micro elastic, structural and mineralization parameters at the tissue level of human femoral bone. Femoral neck models were created based on these data for numerical sound propagation simulations emulating a transverse transmission (TT) setup of an in vivo QUS prototype. In the last part of the project the TT approach has been tested in ex vivo experiments in a rat healing model. The power of QUS, to discriminate two early healing stages has been compared to µCT measurements at the same specimens. It was found that the major contributor to bone adaptation is related to a combination of extracellular matrix elasticity and tissue porosity. It is hypothesized that these parameters are likely to have a considerable impact on the reliability of in silico models. The simulations of the second part confirmed the existence of guided wave propagation in the cortical shell and a high dependency of US parameters on fracture relevant bone properties. The results demonstrate the high potential for bone fracture risk prediction at the femoral neck using QUS. Finally, it was successfully demonstrated that early healing stage discrimination of QUS in TT was superior compared to µCT. In summary these investigations not only show the importance for a precise estimation of micro mechanical properties for numerical modelling but also demonstrate the feasibility and high potential of QUS for bone quality assessment and monitoring of fracture healing.
Li, Jian. "Spontaneous correction of fracture deformity : a study in the rat /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-119-9/.
Повний текст джерелаHillard, Peter John. "Aspects of the biomechanics of Ilizarov external fixation." Thesis, University of Bristol, 1999. http://hdl.handle.net/1983/9934ff7c-a0fc-4eb4-a2ab-323c07024660.
Повний текст джерелаGuo, Qidong. "Healing of bone fracture in type 1 diabetic rat models : a potential gene therapy using bone morphogenetic protein." Thesis, University of Dundee, 2015. https://discovery.dundee.ac.uk/en/studentTheses/8946b0c7-d4b0-4d59-bbcb-ca914643673d.
Повний текст джерелаMurao, Hiroki. "Periosteal cells are a major source of soft callus in bone fracture." Kyoto University, 2014. http://hdl.handle.net/2433/189667.
Повний текст джерелаNewman, Kyle D. "3D Modeling and Finite Element Analysis of Femur After Removing Surgical Screws." OpenSIUC, 2016. https://opensiuc.lib.siu.edu/theses/2025.
Повний текст джерелаShanker, Mihir Daya. "Utilisation of injectable flurochromes for quantification of bone growth in a sheep tibial dynamic fixation model." Thesis, Queensland University of Technology, 2019. https://eprints.qut.edu.au/129179/2/Mihir_Shanker_Thesis.pdf.
Повний текст джерелаFourie, Jeanette Ann. "Stimulation of bone healing in new fractures of the tibial shaft using interferential currents." Master's thesis, University of Cape Town, 1994. http://hdl.handle.net/11427/27041.
Повний текст джерелаSkoglund, Björn. "Following the mevalonate pathway to bone heal alley /." Linköping : Department of Clinical and Experimental Medicine, Materials in Medicine, Section of Orthopaedics and Sports Medicine, Faculty of Health Sciences, Linköping University, 2007. http://www.bibl.liu.se/liupubl/disp/disp2007/med1033s.pdf.
Повний текст джерелаDodds, R. A. "Structural and metabolic studies on normal and pathological bone." Thesis, Brunel University, 1985. http://bura.brunel.ac.uk/handle/2438/4870.
Повний текст джерелаTölli, H. (Hanna). "Reindeer-derived bone protein extract in the healing of bone defects:evaluation of various carrier materials and delivery systems." Doctoral thesis, Oulun yliopisto, 2011. http://urn.fi/urn:isbn:9789514296611.
Повний текст джерелаTiivistelmä Luun paraneminen vaatii erilaisia proteiineja ja kasvutekijöitä. Jos elimistö ei pysty tuottamaan riittävää määrää näitä tekijöitä, luumurtuma ei parane luonnollisesti vaan vaatii hoitoa, jossa murtuma alueelle viedään tarvittavia kasvutekijöitä. Kasvutekijät kiinnitetään tarkoituksenmukaiseen kantaja-aineeseen, jonka avulla kasvutekijöiden vapautumista ja toimintaa voidaan säädellä. Tutkimuksen tavoitteena oli arvioida ja optimoida erilaisissa eläinmalleissa porosta eristetyn luuproteiiniuutteen kantaja-ainesysteemien toimivuutta. Testatut kantaja-ainemateriaalit olivat kollageenihuopa ja bioaktiivinen lasirae. Näitä testattiin useilla luuproteiiniannoksilla, ja mallina oli rotan reisiluun kriittisen koon murtuma. Hiiren reisilihasmallilla testattiin kalsiumsuolayhdistelmiä (beta trikalsiumfosfaatti (β-TCP), hydroksiapatiitti ja kalsiumsulfaatti) tablettina ja pellettinä. Lampaan reisi- ja olkaluiden reikämurtumamallilla testattiin erilaisia β-TCP-rakeita yhdistettynä polyetyleenistä, glyserolista ja steariinihaposta valmistettuun geeliin. Kontrollisryhmistä toinen sisälsi kantaja-aineen ilman luuproteiiniuutetta, toisessa ryhmässä murtuma jätettiin kokonaan hoitamatta. Lammaskokeessa verrattiin lisäksi omaluusiirteen ja demineralisoidun luumateriaalin (DBM) toimivuutta verrattuna poron luuproteiiniuutteeseen. Uudisluun muodostuminen, murtuman paraneminen ja kantaja-aineen hajoaminen arvioitiin natiiviröntgenillä, perifeerisellä tietokonetomografialla (pQCT), mekaanisin testein, histologisesti ja mikro-CT:llä. Luuproteiiniuutetta sisältäneillä ryhmillä oli luun paraneminen ja kantaja-aineen hajoaminen merkittävästi parempaa kuin uutetta sisältämättömillä ryhmillä. Uudisluun muodostuminen oli suurempaa korkeammilla annoksilla ja pitemmillä seuranta-ajoilla. Suurin uudisluun muodostus mitattiin ryhmillä, joissa luuproteiiniuute oli heti käytettävissä implantoinnin jälkeen. Tämä osoittaa, että varsinkin murtuman paranemisen alkuvaiheissa tarvitaan luuta muodostavia kasvutekijöitä riittävinä pitoisuuksina. Mikro-CT-analyysit osoittivat, että luuproteiiniuuttetta sisältäneet ryhmät olivat verrannollisia omaluusiirrehoidolle. Vähiten uudisluuta havaittiin DBM ja tyhjäreikäryhmissä. Tutkimus osoittaa, että poron luuproteiiniuutetta erilaisten kantajamateriaalien kanssa voidaan käyttää parantamaan luun muodostumista. Erityisesti epäorgaaninen kantajamateriaali steariinihapon kanssa on yksi soveltuvimmista vaihtoehdoista luu-uutteelle. Kehitelty pastamutoinen lääkinnällinen laite, joka sisälsi poron luuproteiiniuutteen ja kalsiumsuolakantaja-aineen, osoittautui vaihtoehdoksi omaluusiirrehoidolle
Jernberg, Cassandra. "A Computational Model for Fracture Healing Integrated with Mechanical Stimulation and Growth Factors." Thesis, KTH, Skolan för teknik och hälsa (STH), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-143536.
Повний текст джерелаTufekci, Pelin Melisa. "Development of a novel experimental model to investigate the influence of mechanics on bone healing." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/90730/1/Pelin_Tufekci_Thesis.pdf.
Повний текст джерелаEl-Khassawna, Thaqif. "Cellular and molecular analysis of fracture healing in a neurofibromatosis type 1 conditional knockout mice model." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://dx.doi.org/10.18452/16781.
Повний текст джерелаNeurofibromatosis type 1 (NF1) is an autosomal dominant genetic disease resulting from inactivating mutations in the gene encoding the protein neurofibromin. NF1 patients – around 50% – have abnormalities of the skeleton. Long bones are often affected, and the clinical signs range from tibial bowing to spontaneous fractures and even non-unions. Moreover, NF1 mice models could provide the understanding of the cell types involved in the resulting non-union and their behavior. This study analyzed the healing progress of femur fractures in a model of NF1 long bone dysplasia. Fracture callus was assessed at days 7, 10, 14, and 21 by µCT, histology, biomechanics, and molecular analyses. Bone healing was impaired in Nf1Prx1 mice femoral fracture. Results revealed increased periosteal bone deposition at the early stages of healing, decreased but persistent cartilage formation concomitant with fibrous tissue accumulation within the fracture site, decreased torsional stiffness, decreased bone mineral density, and increased fibrous tissue infiltration in the callus of mutant mice. This fibrous tissue accumulation hindered bone fracture healing, and was deposited by alpha smooth muscle actin-positive myofibroblasts, which were derived from a yet unidentified muscle fascia. This is further supported by the microarray analysis of callus tissues showing that genes crucial to muscle cells physiology, proliferation and differentiation were affected. In addition, extracellular matrix related genes were up-regulated in the mutants. In summary, this study shows a resemblance in the healing progression to the Nf1Prx1 mice model and NF1 patients, thereby, confirming the suitability of this mice model to explore the mechanism by which mutations in NF1 lead to non-unions. Moreover, in vitro and in vivo pilot assessments of MEK inhibitor treatment demonstrated a potential remedy for the lack of neurofibromin in bone healing.
Roberts, C. A. "Trauma and its treatment in British antiquity : An osteoarchaeological study of macroscopic and radiological features of long bone fractures from the historic period with a comparative study of clinical radiographs." Thesis, University of Bradford, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384271.
Повний текст джерелаLow, Adrian Kah Wai Clinical School Prince of Wales Hospital Faculty of Medicine UNSW. "The molecular biology of cancellous bone defects and oestrogen deficiency fractures, in rodents; and the in vivo effects of acid on bone healing." Publisher:University of New South Wales. Clinical School - Prince of Wales Hospital, 2008. http://handle.unsw.edu.au/1959.4/42884.
Повний текст джерелаFountain, Stephanie M. "Monitoring healing progression and characterising the mechanical environment in a preclinical bone defect model." Thesis, Queensland University of Technology, 2016. https://eprints.qut.edu.au/100020/1/Stephanie_Fountain_Thesis.pdf.
Повний текст джерелаLang, Annemarie [Verfasser]. "HIF-stabilization to accelerate fracture healing – Evaluation of a new therapeutic strategy to treat delayed bone regeneration / Annemarie Lang." Berlin : Freie Universität Berlin, 2020. http://d-nb.info/1202778976/34.
Повний текст джерелаWullschleger, Martin Eduard. "Effect of surgical approach on bone vascularisation, fracture and soft tissue healing : comparison of less invasive to open approach." Thesis, Queensland University of Technology, 2010. https://eprints.qut.edu.au/38523/1/Martin_Wullschleger_Thesis.pdf.
Повний текст джерелаKoivukangas, A. (Antti). "Effects of long-term clodronate administration on bone and on fracture healing in rat, with special reference to methodological aspects." Doctoral thesis, University of Oulu, 2002. http://urn.fi/urn:isbn:9514267052.
Повний текст джерелаDuvall, Craig Lewis. "The Role of Osteopontin in Postnatal Vascular Growth: Functional Effects in Ischemic Limb Collateral Vessel Formation and Long Bone Fracture Healing." Diss., Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/14567.
Повний текст джерелаDuvall, Craig L. "The Role of osteopontin in postnatal vascular growth functional effects in ischemic limb collateral vessel formation and long bone fracture healing /." Available online, Georgia Institute of Technology, 2006, 2006. http://etd.gatech.edu/theses/available/etd-01102007-130423/.
Повний текст джерелаDavid Harrison, Committee Member ; Ravi Bellamkonda, Committee Member ; Larry McIntire, Committee Member ; Oskar Skrinjar, Committee Member ; W. Robert Taylor, Committee Chair ; Robert Guldberg, Committee Chair.
Schlundt, Claudia [Verfasser], Georg N. [Gutachter] Duda, Hans-Dieter [Gutachter] Volk, and Andreas [Gutachter] Radbruch. "Impact of the adaptive immune system in bone fracture healing / Claudia Schlundt ; Gutachter: Georg N. Duda, Hans-Dieter Volk, Andreas Radbruch." Berlin : Humboldt-Universität zu Berlin, 2017. http://d-nb.info/1189330059/34.
Повний текст джерелаSkoglund, Björn. "Following the mevalonate pathway to bone heal alley." Doctoral thesis, Linköpings universitet, Ortopedi och idrottsmedicin, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-11282.
Повний текст джерела朱月華 and Yuet-wah Chu. "The use of a Chinese medicinal formula (Chuan-Duan-Bu-Gu-San) on experimental fracture healing in a mouse model." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B31227302.
Повний текст джерелаRohrbach, Daniel [Verfasser], Kay [Akademischer Betreuer] Raum, Hans Dieter [Akademischer Betreuer] Volk, and Detlef [Akademischer Betreuer] Reichert. "Quantitative ultrasound in transverse transmission for bone quality assessment and monitoring fracture healing / Daniel Rohrbach. Gutachter: Kay Raum ; Hans -Dieter Volk ; Detlef Reichert." Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2013. http://d-nb.info/1041937822/34.
Повний текст джерелаIntroini, Simone Orlandi. "Avaliação do reparo tecidual em defeito ósseo por microtomografia tridimensional por raio X." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-11102011-112126/.
Повний текст джерелаFracture is a bone discontinuity that can be surgically produced or caused by an impact that exceeds the mechanical strength of bone by initiating a series of systemic events and specific tissue response. Radiological tests are commonly performed in clinical and animal experiments for monitoring the bone healing by providing information about the alignment of the fragments and the evolution of repair. Other techniques for monitoring quality and quantity can be used in experimental animals (histology and mechanical tests) and in animal experiments and clinical studies (computed tomography X-ray, MRI, ultrasound). The 3D microtomography by X-ray is a new monitoring technique for use in animal experiment and with great potential. The quantification of bone repair with new methods has wide application in research on invasive and noninvasive treatment of fractures using animal experiment. The goal of this research was to use the 3D microtomography by non monochromatic X-ray (\'mü\'CT) to evaluate the bone healing in bone defect in the tibia of Wistar male rat weighing approximately 280 g. The defect was produced by a dental drill with high speed. It was established four experimental groups characterized by the use or non use of low intensity pulsed ultrasound (LIPUS, 30 mW/\'CM POT.2\') for the bone defect treatment as well as the duration of the experiment. In group 1 the LIPUS treatment last 14 days, 5 treatment sessions per week. In group 2 the LIPUS treatment was not used and the duration was 14 days. In group 3 the LIPUS treatment last 21 days, 5 treatment sessions per week. In group 4 the LIPUS treatment was not used and the duration was 21 days. In groups 1 to 4, 10 animals were used for evaluation by CT. The evaluation was conducted by CT through software NRecon, Data Viewer, CT-Vol and CT-Analyzer supplied by the microtomography manufacturer (SkyScan, Belgium). No significant statistical differences were found between the results of groups 1 and 2 as well as the results of groups 3 and 4.
Sant'Anna, Moyses Messias Souza de. "Modulação da expressão dos componentes da matriz extracelular e a modulação celular na regeneração da fratura óssea padronizada em tíbia de ratos." Universidade do Estado do Rio de Janeiro, 2012. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3856.
Повний текст джерелаO propósito do presente trabalho foi investigar a participação da proliferação celular e da expressão dos componentes da matriz extracelular na cascata de eventos do processo de reparo da fratura óssea, empregando as técnicas histológica, imunohistoquímica e morfométrica, em um modelo experimental padronizado para a indução da lesão na tíbia de ratos a partir do método empregado por Yuehuei e Friedman7. É importante padronizar um modelo de indução da fratura, para posterior investigação da participação das células e dos componentes da matriz extracelular no processo de reparo da fratura, considerando que o tempo de consolidação depende significantemente da natureza e do tipo da lesão produzida. Quarenta (n = 40) ratos Wistar foram submetidos a fratura . Os animais foram avaliados em oito (n = 8) grupos de cinco (n = 5) animais, cada grupo emperimental com 12, 24, 48, 72, 96, 144, 192 e 240 horas após a fratura (12h até 10 dias). As fraturas foram classificadas de acordo com o sistema de classificação internacional de fratura de Muller100, AO (Associação para Osteosíntese). Foram encontradas fraturas simples em 86% do total, sendo 68% de fraturas transversas e 18% de fraturas obliquas, 14% do total de fraturas foram complexas, sendo 8% de fraturas irregulares e 6% de fraturas segmentares. Esses resultados demonstram que o aparelho permite padronizar radiológicamente o tipo de fratura, caracterizado pela linha que separa os fragmentos ósseos. Os resultados qualitativos dos componentes da matriz extracelular para TGF-β, VEGF, colágeno I e II, osteopontina, proteoglicanos, fibras do sistema elástico com a coloração de resorcina funcsina de Weigert, e para proliferação celular pelo PCNA, assim como os resultados morfométricos, sugerem que a modulação da expressão dos componentes da matriz extracelular e a proliferação celular durante o processo de reparo da fratura não é homogênea para todos os componentes teciduais, dependendo significantemente das tensões locais geradas pelo tipo da linha de fratura que pode ser determinante no tempo de regeneração do osso e na qualidade da restauração das propriedades biomecânica. Nossos achados podem contribuir para melhor compreensão da reparo de fratura óssea e para novas abordagens terapêuticas que considerem as propriedades biomecânicas do tecido ósseo em reparo nas suas diferentes etapas
The purpose of this study was to investigate the role of cells proliferation and extracellular matrix components expression in the process of bone fracture repair. To do so it used histological techniques, immunohistochemistry and morphometric analysis as well as a standardized experimental model for the induction of injury to the tibia of rats as proposed by Yuehuei and Friedman7. It is important to standardize a model of fracture induction for further investigation of the involvement of cells and extracellular matrix components in the fracture repair process, whereas the healing time depends significantly on the nature and type of lesion produced. Forty (n = 40) Wistar rats were subjected to fracture. The animals were divided into eight (n = 8) groups of five (n = 5). Each subgroup was observed after 12, 24, 48, 72, 96, 144, 192 and 240 hours of fracture (12 to 10 days). Immediately afterwards, the fractures were classified according to the system of international classification of fracture by Muller100, AO (Association for Osteosynthesis). Simple fractures were found in 86% of the total, among them, 68% were transverse and 18% were oblique. Complex fractures were found in 14% of the cases, among them 8% were irregular and 6% were segmental. These results demonstrated that the device enables researchers to standardize the type of fracture by X-ray, marked by the line separating the bone fragments. The qualitative results of the cells and extracellular matrix components of TGF-β, VEGF, PCNA, collagen I and II, osteopontin, proteoglycans, elastic fibers system with resorcin funcsin of Weigert, as well as the morphometric results suggest that the repair process of the fracture is not homogeneous for all components. Expression of the extracellular matrix components and cell proliferation modulation significantly depends on the local stresses generated by the type of the fracture. Such type can be decisive in determining time duration for bone regeneration and quality of the biomechanical properties restoration. Our findings may contribute to better understanding of bone fracture repair and for new therapeutic approaches that consider the biomechanical properties of bone tissue in repair in its different stages
Yamanaka, Jéssica Suzuki. "Efeitos da dieta hiperlipídica na estrutura e consolidação de tíbias de ratas em crescimento." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17142/tde-07062017-165223/.
Повний текст джерелаThe body fat accumulation have been considered a public health issue with several negative consequences, including reduction of bone quality, that affects young people increasingly. The studies that seek to clarify the relationship between fat mass and bone mass have been increasing. Therefore the aim of this study was to investigate the effect of high consumption of lipids on bone structure and bone healing in growing rats. Fifty-two female Wistar rats 21 days old were used, divided into four groups (n=13), as follows: animals treated with standard diet (SD), animals treated with high-fat diet (HFD), animals treated with standard diet and osteotomy (SDO) and animals treated with high-fat diet and osteotomy (HFDO). Experimental period was of 5 weeks for every groups. The SD and HFD animals were maintained without intervention, while SDO and HFDO groups were submitted to osteotomy in left tibia at third experimental week. Animal body mass and feed intake were assessed during experimental period. One day before euthanasia insulin tolerance test was performed in 5 animal of each group. After euthanasia, the left tibia of each rat was dissected and cleaned of soft tissues and submitted to macroscopy analysis, densitometry, mechanical analysis, histomorphometry, stereology of newly formed bone and immunohistochemistry. Data were analyzed by general linear model with adjustment to multiple comparisons of Bonferroni for comparisons between variables and TStudent e Mann-Whitney test for comparisons between two groups. At end of the experiment the high-fat diet fed animals had body mass similar to standard diet fed animals (p=0.115), however they had lower feed intake (p<0.001) and lower insulin sensibility (p=0.018). Surgery affected feed intake, operated animals had lower feed intake than non-operated animals (p=0.017). High-fat diet fed animals had longer tibias (p=0,041), lower maximal load (p<0.001) and lower stiffness (p=0,003) than standard diet fed animals, although bone mineral density (BMD) had been similar (p=0,958). Operated animals had lower maximal load (p<0.001), stiffness (p=0.020) and lower collagen quantity (p=0.029) than non-operated animals. Newly formed bone of tibias submitted to osteotomy trended to be lower in HFDO group than SDO group (p=0.054). Immunohistochemistry presented no difference between HFDO e SDO group (p<0.05). We concluded high-fat diet affected bone in order to harm tibia strength and caused a decrease in the newly formed bone volume, impairing structure and bone healing
Lenza, Mário [UNIFESP]. "Intervenções para o tratamento de fratura e pseudartrose da clavícula em adultos e adolescentes: revisão sistemática." Universidade Federal de São Paulo (UNIFESP), 2010. http://repositorio.unifesp.br/handle/11600/9772.
Повний текст джерелаObjetivo: Avaliar a efetividade dos diferentes métodos de tratamento da fratura e pseudartrose do terço médio da clavícula em adultos e adolescentes. Métodos: Estratégia de busca: abrangeu CENTRAL, MEDLINE, EMBASE e LILACS. Não houve restrições de idioma ou meios de publicações. A última estratégia de busca foi realizada em julho de 2009. Critério de seleção: foram incluídos ensaios clínicos randomizados e quase-randomizados que avaliaram o tratamento de fratura aguda e pseudartrose do terço médio da clavícula em adultos e adolescentes. Os desfechos primários foram: dor, qualidade de vida/função do ombro e falha do tratamento. Coleta e análise dos dados: dois autores, independentemente, selecionaram os estudos elegíveis, avaliaram a qualidade metodológica e extraíram os dados. Calculou-se o risco relativo com 95% de intervalo de confiança para as variáveis dicotômicas; para variáveis contínuas, a diferença entre as médias foi calculada com 95% de intervalo de confiança. Quando possível, os estudos foram agrupados. Resultados: Intervenções não cirúrgicas: dois estudos compararam imobilização em oito versus tipoia. Ambos possuíam baixo poder estatístico e alto risco de viés. Houve maiores níveis de dor e desconforto durante o tratamento nos pacientes submetidos à imobilização em oito. Um terceiro estudo, com baixo risco de viés, mas baixo poder estatístico, avaliou o ultrassom terapêutico. Não houve diferenças significantes entre o ultrassom de baixa intensidade e placebo nos desfechos avaliados. Intervenções não cirúrgicas versus cirúrgicas: Quatro estudos, dois com moderado e dois com alto risco de viés foram incluídos. Dois estudos compararam fixação com placa versus tipoia, com resultados favoráveis ao tratamento cirúrgico ao avaliar falha do tratamento e qualidade de vida/função do ombro. Outros dois estudos compararam fixação intramedular versus tratamento não cirúrgico; a cirurgia foi superior para os desfechos dor e qualidade de vida/função do ombro. Intervenções cirúrgicas: quatro estudos com baixo poder estatístico, cada qual avaliando diferentes comparações, foram incluídos; três possuíam alto risco de viés. Um estudo comparou placa de compressão de baixo contato com placa de compressão dinâmica em pseudartrose da clavícula; os pacientes tratados com placa de baixo contato apresentaram evolução melhor para: função do ombro, consolidação, retorno ao trabalho e menor incidência de sintomas relacionados ao implante. Outro estudo comparou fixação intramedular com pinos de Knowles versus fixação com placa para o tratamento de fratura aguda e pseudartrose da clavícula; a fixação intramedular acarretou em menor consumo de analgésicos após a cirurgia, menor número de complicações associadas ao implante e menor tempo cirúrgico e de internação. Um terceiro estudo, com baixo risco de viés, concluiu que a técnica de fixação tridimensional com placa ocasionou menor incidência de retardo de consolidação em relação à fixação na face superior das fraturas agudas da clavícula. Um último estudo avaliou a fixação intramedular das fraturas agudas da clavícula comparando redução fechada versus redução aberta. Houve diferenças significativas em favor da redução fechada quando avaliados os desfechos primários. Conclusão: os ensaios clínicos disponíveis na literatura não apresentam qualidade metodológica adequada e/ou poder estatístico apropriado, portanto, não há evidência suficiente para determinar quais os mais apropriados métodos de tratamento para a fratura e pseudartrose do terço médio da clavícula. Há uma tendência de melhores resultados funcionais e radiográficos a favor das intervenções cirúrgicas quando comparadas às intervenções não cirúrgicas.
Objectives: To evaluate the effectiveness of different methods of treatment for acute fracture or non-union of the middle third of the clavicle in adults and adolescents. Methods: Search strategy: We searched the Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, LILACS, trial registries and reference lists of articles. No language or publication restrictions were applied. Selection criteria: Randomised and quasi-randomised controlled trials evaluating any intervention for treating fractures or non-union of the middle third of the clavicle were considered. The primary outcomes were pain, treatment failure and health-related quality of life or shoulder function. Data collection and analysis: Two authors independently selected eligible trials and three authors assessed methodological quality and cross-checked data extraction. Results: Conservative interventions: Three trials were included in this comparison. Two trials compared the figure-of-eight bandage with sling in a total of 234 participants. Both trials were underpowered and compromised by poor methodology. One trial found slightly higher pain levels in the bandage group at 15 days, and the other trial reported greater discomfort during bandage wear. There were no significant differences in functional or other outcomes reported for either trial. The third trial, which evaluated therapeutic ultrasound in 120 participants, was also underpowered but had a low risk of bias. The trial found no statistically significant difference between low-intensity pulsed ultrasound and placebo in the time to clinical fracture healing or in any of the other reported outcomes. Surgical versus conservative interventions: Four studies, two with moderate and two with high risk of bias were included. Two compared plate fixation versus sling; plate fixation showed better patient-based upper extremity outcome scores and less treatment failure. Other two trials comparing intramedullary fixation versus conservative interventions presented better upper limb function to the surgery treatment. Surgical interventions: Data from four small trials, each testing a different comparison, were included. Three trials had design features that carry a high risk of bias, limiting the strength of their findings. Low-contact dynamic compression plates appeared to be associated with significantly better upper-limb function throughout the year following surgery, earlier fracture union and return to work, and a reduced incidence of implant-associated symptoms when compared with a standard dynamic compression plate in 36 adults with symptomatic non-union of the middle third of the clavicle. One study (69 participants) compared the Knowles pin versus plate for treating middle third clavicle fractures or non-union. Knowles pins appeared to be associated with lower pain levels and use of post-operative analgesics, reduced incidence of implant-associated symptoms, and shorter operation time and hospital stay. One study (133 participants) found that a three-dimensional technique for fixation with a reconstruction plate was associated with a significantly lower incidence of symptomatic delayed union than a standard superior position surgical approach. One study (201 participants) assessed the intramedullary fixation for treating acute clavicle fractures comparing closed and opened reduction; there were statistical significant differences in favour of closed reduction with percutaneous fixation for the primary outcomes. Conclusion: There is limited evidence, from single trials only, regarding the effectiveness of different methods for treating fracture and non-union of the middle third of the clavicle. Further research is warranted.
TEDE
BV UNIFESP: Teses e dissertações
Milanetti, Marcia Regina. "Avaliação do reparo ósseo em osteotomia experimental por microtomografia por raio-X." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/82/82131/tde-20072012-154325/.
Повний текст джерелаX-ray microtomography (uCT) provides quantitative and three dimensional measurements of the callus structure and these measurements could potentially be related to callus strength. The assessment of bone repair through new methodologies has important application in animal investigations regarding invasive or non invasive technologies for the stimulation of bone healing. The aim of this investigation was the use of \'mü\'CT for the assessment of bone repair in a rat femur bone defect. Thirty male Wistar rats weighting about 300g were divided in three experimental groups with 10 animals on each group. The animals were anesthetized and a circular hole with a 1.2 mm diameter was generated at the medial region of the left femur using a dental drill. At the 7th, 14th and 21st day after surgery the animals of experimental groups 1, 2 and 3, respectively, were sacrificed and the left femur excised. The femurs were wrapped in gauze immersed in phosphate-buffered solution and stored in a plastic bag at -20ºC until the analysis by microtomograph. The femurs were scanned by the 1172 microtomograph (SkyScan, Belgium). The softwares NRecon, Dataviewer, CT-Analyzer and CT-Vol, provided by the microtomograph manufacture, were used for the following assessments: a) visual examination of the femurs microtomographic reconstructions using transversal, coronal and sagittal sections; b) segmentation of the bone callus in the reconstructions using an image processing algorithm to quantify the parameters total bone callus volume (TV), volume of the mineralized bone callus (BV), the ratio BV/TV and the volumetric bone callus mineral density (BMD); c) 3D rendering of the bone callus. The statistical analysis of the measured parameters was performed by the Student t test with a level of significance p<0,05. There was a statistically significant increase in the mean values of the parameters BV/TV and BMD in the comparison of experimental groups 1 and 2. The comparison between groups 2 and 3 was not statistically significant. Results are according to bone physiology data from literature although the image processing algorithm used needs some adjustments to get better results in bone callus segmentation in ROI.
Reis, João Pedro Silva dos. "Caracterização da aplicação de placas bloqueadas Fixin® no maneio de fracturas apendiculares em canídeos." Master's thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2014. http://hdl.handle.net/10400.5/6913.
Повний текст джерелаUm dos objectivos do desenvolvimento de implantes ósseos é a resolução de fracturas, tornando, novamente, o membro funcional. Para tal, os implantes necessitam de auxiliar os tecidos a recuperar a sua integridade biológica e mecânica. As placas bloqueadas têm vindo a ser introduzidas no material ortopédico do cirurgião veterinário para técnicas de osteossíntese. Se por um lado as placas convencionais têm como objectivo obter o máximo de estabilidade através de uma placa rígida e de compressão dos fragmentos, por outro, as placas bloqueadas permitem manter uma certa elasticidade. Este parâmetro, em combinação com o principal objectivo das placas bloqueadas, que é a preservação da vascularização e dos tecidos moles que envolvem a fractura, promovem a formação de calo ósseo e por consequência, uma cicatrização óssea indirecta. O sistema Fixin® diferencia-se pela utilização de uma bucha nos orifícios, que realiza uma ligação cónica aos parafusos. A própria bucha permite a utilização de placas mais finas e facilita a alteração e remoção do implante do osso. Este estudo retrospectivo, teve como objectivo a caracterização da aplicação de placas bloqueadas, pertencentes ao sistema Fixin®, numa população de vinte e sete indivíduos, vinte e seis pertencentes à espécie Canis familiaris e um à espécie Felis catus, no Hospital Escolar Veterinário da Faculdade de Medicina Veterinária da Universidade de Lisboa. Para cada paciente, foi caracterizada a fractura e o implante utilizado, registando-se, no período pós-cirúrgico, o resultado clínico, o resultado radiográfico e, em caso de ocorrência, complicações. No final, todos os pacientes apresentaram-se a apoiar o membro, nenhum com sinais de dor e apenas um apresentou sinais de claudicação. A totalidade dos casos que se apresentaram a exame radiográfico (n=13), demonstraram união óssea. Complicações maiores foram observadas em sete fracturas (26%). Após a realização de um inquérito aos proprietários, 87% demonstraram-se muito satisfeitos com o resultado cirúrgico. Apesar de, neste estudo, ter ocorrido um valor moderado de complicações, as placas Fixin® demonstraram ser um sistema de osteossíntese aceitável, apresentando-se como uma escolha com potencial para a resolução de fracturas apendiculares.
ABSTRACT - Characterization of Fixin® locking plates application in the management of appendicular fractures in dogs - One purpose of the development of bone implants is fractures resolution, making the limb functional again. With that in mind, implants need to assist the recover of the tissues biological and mechanical integrity. In recent years, locking plates have been introduced in the veterinary surgeon orthopedic material for osteosynthesis techniques. If on one hand, conventional plates are designed to get maximum stability through a stiff plate and compression of the fragments, on the other, locking plates allow to keep a certain elasticity. Elasticity, combined with the main purpose of the locking plates, which is the preservation of the vasculature and soft tissues surrounding the fracture, promote callus formation and, therefore, an indirect bone healing. The Fixin® system is distinguished by the use of bushing-inserts in the holes, which are conically shaped to engage and secure the head of the screw. Bushing-inserts allow the use of thinner plates and facilitate modifications and implants removals. This retrospective study aimed to evaluate the use of locking plates, belonging to the Fixin® system, in a population of twenty seven individuals, twenty six belonging to Canis familiaris and one Felis catus, from de Hospital Escolar Veterinário from Faculdade de Medicina Veterinária of Universidade de Lisboa. For each patient, data pertaining to the fracture and the implants used, were recorded. Postoperatively, the clinical and radiographic outcomes and complications were analyzed. In the end, limb support was demonstrated by all patients, with no signs of pain and a single case of lameness was recorded. All the patients who were submitted to radiographic evaluations (n=13), showed bone union. Major complications were seen in seven fractures (26%). Eighty seven percent of the owners were very pleased with the surgical outcome. Despite of a modest complications rate, the Fixin® locking plates have demonstrated to be an acceptable osteosynthesis system and thus a potential choice of implant for stabilization of appendicular fractures.