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Pompeu Junior, Jorgino, Silvia Blumer, and Marcos Deon Vilela de Resende. "Avaliação genética de seleções e híbridos de limões cravo, volkameriano e rugoso como porta-enxertos para laranjeiras Valência na presença da morte súbita dos citros." Revista Brasileira de Fruticultura 35, no. 1 (March 2013): 199–209. http://dx.doi.org/10.1590/s0100-29452013000100023.
Повний текст джерелаАнотація:
Este estudo teve como objetivo realizar a avaliação genética da produção de frutos, eficiência produtiva e altura de laranjeiras Valência (Citrus sinensis) enxertadas em seleções e híbridos dos limões Cravo (C. limonia), Volkameriano (C. volkameriana) e Rugoso (C. jambhiri), em área endêmica para morte súbita dos citros (MSC). Foram avaliados 36 genótipos desses porta-enxertos, representados por cinco plantas cada, avaliados em cinco safras, do terceiro ao sétimo ano após o plantio. Sete dos genótipos avaliados apresentaram plantas com sintomas de MSC até o sétimo ano: Rangpur Otaheite orange 12901 (859), Rangpur Red Lime D.33.30 (866), Limão-Cravo EEL (871), Rangpur Borneo red (874), Citrus kokhai (1649), Limão-Rugoso 58329 (1655) e Limão- Cravo x Swingle B (1695). Para os genótipos que não manifestaram sintomas da doença, foram estimados parâmetros genéticos e fenotípicos e realizada a predição de valores genéticos dos indivíduos, visando à seleção e ao melhoramento genético para as características citadas, empregando-se o método REML/BLUP (máxima verossimilhança restrita/melhor predição linear não viciada). A análise de produção de frutos de cinco safras mostrou acurácia seletiva de 84,59%, tornando-se desnecessária a avaliação de maior número de safras. A seleção dos sete melhores genótipos proporcionou ganhos genéticos de 11,5% na produção de frutos, enquanto a do melhor genótipo conferiu ganho genético de 16,3%. As maiores médias genéticas preditas (>70,0 kg.pl-1) para produção de frutos foram obtidas pelos genótipos Limão-Cravo- Ipanema (1522), Santa- Bárbara-Red- Lime (884), Limão- Cravo- Limeira (863), Limão- Cravo- Taquaritinga (869), Limão- Rugoso- do -Cabo (1643), Rangpur- Rose Lime (868) e Limão- Cravo- da- Califórnia (1467). Já a acurácia seletiva da eficiência produtiva, para quatro colheitas, foi 77,4%. Para este caráter, as maiores médias genéticas (>8,0 kg.m-3) foram dos genótipos Rangpur- Lime x Trifoliata 3810 (1648), Rangpur- Lime x Trifoliata 5320 (1644), Limão- Cravo x Citrange- Carrizo (1524), Citrus pennivesiculata (880), Limão- Cravo x Trifoliata- Swingle A (1707), Rangpur- Rose- Lemon 124684 (864), Rangpur- Red -Lime D33.47 (867) e Limão- Cravo -Ipanema (1522). Dentre os 10 melhores genótipos para produção de frutos e para eficiência produtiva, apenas três são coincidentes: Rangpur- Rose -Lime (868), Citrus pennivesiculata (880) e Limão- Cravo-Ipanema (1522).
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VOORBERG, Jan, Rozalia T. M. de LAAF, Pauline M. KOSTER, and Jan A. van MOURIK. "Intracellular retention of a factor VIII protein with an Arg2307>Gln mutation as a cause of haemophilia A." Biochemical Journal 318, no. 3 (September 15, 1996): 931–37. http://dx.doi.org/10.1042/bj3180931.
Повний текст джерелаАнотація:
Substitution of Arg2307 by Gln in factor VIII has been found to be associated with mild to moderate haemophilia A [Gitschier, Wood, Shuman and Lawn (1986) Science 232, 1415–1416]. We have introduced this particular point mutation into a B-domain-deleted factor VIII cDNA and expressed the modified cDNA in C127 cells. Cells expressing the resulting protein, termed des-(868–1562)-factor VIII-R2307Q, were compared with those expressing the previously characterized des-(868–1562)-factor VIII. No immunoreactive material could be detected in the conditioned medium of cells transfected with des-(868–1562)-factor VIII-R2307Q cDNA using assays specific for the factor VIII light chain and the factor VIII heavy chain. Analysis of metabolically labelled cells transfected with des-(868–1562)-factor VIII-R2307Q cDNA revealed that this mutant protein is synthesized at a level similar to des-(868–1562)-factor VIII. In contrast to des-(868–1562)-factor VIII, metabolically labelled des-(868–1562)-factor VIII-R2307Q was not encountered in the conditioned medium of the transfected cells, indicating that the mutant protein is not secreted from the cell. Inspection of the intracellular localization of the two proteins in the cell employing morphological analysis, endoglycosidase H and experiments with inhibitors of glucosidases I and II was consistent with localization of des-(868–1562)-factor VIII and des-(868–1562)-factor VIII-R2307Q in the endoplasmic reticulum. Taken together, our data indicate that the Arg2307 → Gln mutation results in aberrant intracellular trafficking of factor VIII, which may explain the low levels of factor VIII antigen in the plasma of haemophilia A patients that carry this particular point mutation.
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AKBAŞ, Mustafa Yasin. "Ferhat Gökçe. İsnâdlarda Saklı Eserler Humeyd b. Zancûya'nın (d. 251/868) Kitâbu’t-Tergîb’inin Yeniden İnşâsı (Ankara: İlahiyat, 2019)." ULUM 3, no. 2 (December 31, 2020): 487–95. http://dx.doi.org/10.54659/ulum.807082.
Повний текст джерелаАнотація:
Değerlendirmesini sunduğumuz İsnâdlarda Saklı Eserler Humeyd b. Zencûye’nin (ö. 251/868) Kitâbu’t-Tergîb’inin Yeniden İnşâsı başlıklı kitap, giriş ve üç bölümden oluşmaktadır: “Humeyd b. Zencûye’nin hayatı, ilmî kişi-liği ve eserleri”, “Kitâbu’t-Terğîb’in yeniden inşâsı” ve “Kitâbu’t-Tergîb”. Yazar, günümüze ulaştığı bilin-meyen İbn Zencûye’nin bu eserinin izini, çeşitli kaynaklardan takip etmiştir. Bu çalışma ile hem İbn Zencûye’nin eseri hakkında fikir sahibi olabiliyor hem de başta hadis olmak üzere İslam ilim geleneğinin sahip olduğu zenginliği görebiliyoruz.
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WADA, Naoya, and Hideaki FURUKAWA. "Photonic Network Technologies for New Generation Network." IEICE Transactions on Communications E94-B, no. 4 (2011): 868–75. http://dx.doi.org/10.1587/transcom.e94.b.868.
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Akermann, Manfred, and Staatsarchiv (Ludwigsburg) Abteilung Landesbeschreibung. "Rezension von: Staatsarchiv Ludwigsburg. Abteilung Landesbeschreibung (Bearb.), B. Gemeindebeschreibungen Ehingen bis Westerstetten ..." Württembergisch Franken 77 (August 31, 2023): 537. http://dx.doi.org/10.53458/wfr.v77i.7559.
Повний текст джерелаАнотація:
Der Alb-Donau-Kreis. Bd. II, Teil B. Gemeindebeschreibungen Ehingen bis Westerstetten. Hrsg. v.d. Landesarchivdirektion Baden-Württemberg in Verb, mit dem Alb-Donau-Kreis. Bearb. v.d. Abt. Landesbeschreibung des Staatsarchivs Ludwigsburg. - Sigmaringen: Thorbecke, 1992. 1064 S., zahlr. Abb. Karten, Statistik, Register u.a. in Mappe beil.Der Neckar-Odenwald-Kreis. Hrsg. v.d. Landesarchivdirektion Baden-Württemberg in Verb, mit dem Neckar-Odenwald-Kreis. Bearb. v.d. Abt. Landesbeschreibung des Generallandesarchivs Karlsruhe. Bd. I, Teil A. Allgemeiner Teil; Teil B. Gemeindebeschreibungen Adelsheim bis Höpfingen. Bd. II, Teil B. Gemeindebeschreibungen Hüffenhardt bis Zwingenberg. - Sigmaringen: Thorbecke, 1992. 920 bzw. 868 S., zahlr. Abb. Karten, Statistik u.a. in Mappe beil.
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Lee, Seung-Kwan, Deuk-Soo Jun, Dong-Keun Lee, and Jong-Min Baik. "Clinical Characteristics of Elderly People with Osteoporotic Vertebral Compression Fracture Based on a 12-Year Single-Center Experience in Korea." Geriatrics 7, no. 6 (October 31, 2022): 123. http://dx.doi.org/10.3390/geriatrics7060123.
Повний текст джерелаАнотація:
In an aging human population, osteoporotic vertebral compression fracture (OVCF) frequently occurs. We conducted this retrospective study to analyze the clinical characteristics of elderly people with OVCF who underwent percutaneous vertebroplasty or kyphoplasty over a 12-year period at a single medical center in Korea. Between 2007 and 2019, A total of 868 patients (n = 868) were treated at our institution. We assessed 600 of these patients as eligible for study purposes and divided them into three groups: Group A (spine and hip T-scores ≤−2.5; n = 332); Group B (spine T-scores ≤−2.5; n = 189); and Group C (hip T-scores ≤−2.5; n = 79). The baseline characteristics of the patients included age, sex, body mass index (BMI), past history of steroid use, alcohol consumption, use of osteoporosis therapy, smoking, and treatment for OVCF. We compared these characteristics between the three groups. We found that the mean patient age was significantly higher in Group A, compared with Group B, and significantly lower in Group B, compared with Group C. We also found significant differences in the male-to-female ratio and mean body mass index between the three groups. In conclusion, we suggest that special attention should be paid to factors closely associated with spine and hip T-scores when evaluating elderly people with OVCF and determining appropriate treatment.
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KIM, D. S., H. M. SEO, S. Y. LEE, Y. K. MOON, B. S. KIM, T. H. HWANG, and D. J. CHUNG. "A Fully Integrated SoC with Digital MAC Processor and Transceiver for Ubiquitous Sensor Network at 868/915 MHz." IEICE Transactions on Communications E90-B, no. 12 (December 1, 2007): 3336–45. http://dx.doi.org/10.1093/ietcom/e90-b.12.3336.
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Schäfer, Helene, Karthikeyan Subbarayan, and Barbara Seliger. "868 PRELP-facilitated enhancement of MHC class I surface expression in B16F10 melanoma cells." Journal for ImmunoTherapy of Cancer 8, Suppl 3 (November 2020): A920. http://dx.doi.org/10.1136/jitc-2020-sitc2020.0868.
Повний текст джерелаАнотація:
BackgroundPRELP (proline arginine-rich end leucine-rich repeat protein; also Prolargin), a small leucine-rich proteoglycan, functions as a molecule anchoring basement membranes to connective tissues via the interaction with collagens and heparin. PRELP facilitates the binding of cells to glycosaminoglycans as an important regulator of cell adhesion and thus displays pathophysiological features. Melanoma is an immunogenic tumor, whose relationship with immune cells resident in the microenvironment significantly influences cancer cell proliferation, progression and metastasis. Evasion from immune surveillance is a hallmark of melanoma progression. While our laboratory reported that the proteoglycan biglycan (BGN) was enhancing MHC class I in tumor cells,1 the role of PRELP in tumor immunology has not been studied.MethodsThe murine metastatic melanoma cell line B16F10, characterized by a reduced expression of MHC class I surface antigens was chosen for this study. B16F10 cells were transiently transfected with PRELP as well as co-transfected with BGN. Expression of antigen processing machinery (APM) components and PRELP was determined by qPCR and MHC class I surface expression by flow cytometry. Promoter activity of APM components was analysed by luciferase reporter assays. XTT assays were used to determine cell proliferation. The association of PRELP and MHC class I was studied by bioinformatics in a mixed melanoma dataset of 83 samples.2ResultsOver-expression of PRELP in B16F10 cells enhanced the expression of MHC class I surface antigens, which was due to a PRELP-mediated transcriptional upregulation of components of the MHC class I APM components TAP1, TAP2 and TAPBP as determined by qPCR and promoter assay in PRELP transfectants versus mock controls. Furthermore, MHC class I surface expression was even more pronounced upon BGN co-transfection with PRELP. PRELP overexpression was able to inhibit the proliferation of the B16F10 cells. Bioinformatics analyses demonstrated a positive correlation of PRELP with HLA-A , -B and -C alleles in human melanoma.ConclusionsOur findings demonstrated that overexpression of PRELP correlates with higher MHC class I expression and inhibits cell proliferation. For the first time, co-transfections of the two proteoglycans PRELP and BGN had a synergistic effect on upregulating MHC class I expression. Therefore, PRELP can serve as a novel therapeutic strategy that deserves further investigation.AcknowledgementsThe project is supported by Wilhelm-Sander-Stiftung (No: 2019.076.1) and by a Roux grant (FKZ: PK37) of the Medical Faculty of the Martin-Luther-University Halle-Wittenberg.ReferencesSubbarayan K, Leisz S, Wickenhauser C, Bethmann D, Massa C, Steven A, Seliger B. Biglycan-mediated upregulation of MHC class I expression in HER-2/neu-transformed cells. Oncoimmunology 2018;7:e1373233.Xu L, Shen SS, Hoshida Y, Subramanian A, Ross K, Brunet JP, Wagner SN, Ramaswamy S, Mesirov JP, Hynes RO. Gene expression changes in an animal melanoma model correlate with aggressiveness of human melanoma metastases. Molecular Cancer Research 2008;6:760-769.
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Dybski, Henryk. "Wydania krytyczne, czas powstania i autorzy starożytnych "Żywotów" św. Teodora Studyty. Jego curriculum vitae w oparciu o "Żywot A"." Vox Patrum 57 (June 15, 2012): 137–54. http://dx.doi.org/10.31743/vp.4123.
Повний текст джерелаАнотація:
The article states that the four ancient Greek lives (A, B, C, D) contain extensive information about the life and activity of St. Theodore of Studios (759-826). The oldest one is Vita S. Theodori Studitae B which was published in 1853 by A. Mai. The document was written in c. 868 and its author was probably a monk Michael or another ascetic who lived in the Studios Monastery in Constantinople; Vita A, which is an extended version of Vita B, was published in 1696. His author was Theodore Daphnopates, who lived in the first half of the 10th century. Vita C (Vita S. Theodori Studitae in codice Mosquensi musei Rumianzoviani No 520) was published in 1915, but it dates back to the 12th century and it is similar in its content to Vita S. Theodori Studitae A and B. Vita D (Codex Monacensis No 467) was not yet published.
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Wang, San Xin, and Ze Yu Wu. "Magnetic Properties and Glass Forming Ability of Fe-B-Y-Nb-Zr Bulk Metallic Glasses." Advanced Materials Research 577 (October 2012): 27–30. http://dx.doi.org/10.4028/www.scientific.net/amr.577.27.
Повний текст джерелаАнотація:
Bulk metallic glasses (BMGs) alloys (Fe71.2B24Y4.8)96Nb4-xZrx (x = 1-4 at.%) with a maximum diameter of 5 mm were synthesized with low purity materials by copper mold casting technique. The effect of Zr substitution for Nb on the structure, thermal stability and the magnetic properties has been studied. It was found that the substitution of an appropriate amount of Zr for Nb can improve the glass forming ability. The wide supercooled liquid region ∆Tx (exceeding 123 K) and a high Tg (exceeding 868 K) demonstrated a high thermal stability of the present Fe-based BMGs. In addition, these BMGs also exhibit good soft magnetic properties with relatively high saturation magnetization of 88 emu/g, and low coercivity of 20 Oe.
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Subardjo, Euis Rahma Nursyifa, Wandy Zulkarnaen, and Asep Suwarna. "Pengaruh Lingkungan Kerja Terhadap Kinerja Karyawan (Studi Pada Divisi F&B Yogya Sunda Heritage 60 Bandung)." Jurnal Manajemen DIVERSIFIKASI 3, no. 3 (September 12, 2023): 493–500. http://dx.doi.org/10.24127/diversifikasi.v3i3.4482.
Повний текст джерелаАнотація:
This research was conducted at the F&B Division Of Yogya Sunda Heritage 60 Bandungwhich is a company engaged in retail, food and beverage production. The purpose of thisstudy was to analyse the effect of the work environment on employee performance todetermine the constraints on employee performance and provide solutions to theseconstraints. The method used in this research is to use verification and descriptive researchwith a quantitative approach, and data collection techniques through questionnaires,literature studies, interviews and observations. This method can help to measure the effect ofa variable by using SPSS data processing. The sample of this research is 32 respondents. Theresults of the analysis show that, based on the t-test, there is a significant positive effectbetween the work environment on the performance of the employees of the F&B Division OfYogya Sunda Heritage 60 Bandung indicating that the t value > t table (14,044 > 1,684).Thus Ho is rejected and Ha is accepted, which means that there is a significant influencebetween the work environment on employee performance. That is, the higher the workenvironment, the higher the performance. To determine the magnitude of the influence of thework environment on performance, it can be seen in the R Square column. The amount of RJurnal Manajemen DiversifikasiVol. 3. No. 3 (2023)494Square (R2) is 868. So it can be concluded that the work environment can contribute 86.8%to employee performance. And the other 13.2% are still influenced by other factors.
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Çam, Sedat, Robin Brinkmeyer, and John R. Schwarz. "Quantitative PCR enumeration of vcgC and 16S rRNA type A and B genes as virulence indicators for environmental and clinical strains of Vibrio vulnificus in Galveston Bay oysters." Canadian Journal of Microbiology 65, no. 8 (August 2019): 613–21. http://dx.doi.org/10.1139/cjm-2018-0399.
Повний текст джерелаАнотація:
Oysters from a reef in Galveston Bay, Texas, USA, were screened for more virulent clinical strains versus less virulent environmental strains of Vibrio vulnificus using a combination of quantitative PCR assays for the virulence correlating gene (clinical variant, vcgC) and 16S rRNA types A and B (type A = environmental, type B = clinical). The combination of vcgC and 16S rRNA type B loci to determine clinical type strains was suitable, as indicated by the strong correlation (R2 = 0.98; p < 0.001) between these gene counts over time and their relative proportion (up to 93.8% and 94.3%, respectively) to vvhA genes used to quantify all strains of V. vulnificus. A strong seasonal shift of V. vulnificus strain types was observed. Environmental strains (16S rRNA type A) predominated from April to mid-June as salinities increased from 22 to 27 PSU (practical salinity unit) and temperatures rose 20 to 28 °C, with peak gene quantities of 16 812 ± 56 CFU/g. As temperatures increased to ≥30 °C from mid-June to September and salinities rose above 27 PSU, clinical strains (16S rRNA type B; vcgC) predominated with peak quantities 31 868 ± 287 and 32 360 ± 178 CFU/g, respectively.
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Gervain, Judit. "Implications of the changes in the levels of HBV DNA and HBsAg during the treatment of chronic hepatitis B." Orvosi Hetilap 152, no. 22 (May 2011): 866–68. http://dx.doi.org/10.1556/oh.2011.29124.
Повний текст джерелаАнотація:
With the development of new drugs, there has been a significant progression in the treatment of viral hepatitis B over the past five years. Based on their effect mechanisms, the currently available seven different drugs can be classified either as those of the interferon group or those of the nucleoside analogue group. Despite the pharmacological advances, it is still rare to achieve sustained response. The chances of a long-term inactive hepatitis stage, however, have greatly increased with the growing opportunities for personalized pharmacological treatment based on the selection of the correct type of drug, the timely modification of therapy in case of ineffectiveness and the determination of the optimal time and length of the therapy. For this, it is necessary to monitor several predictive, non-invasive biomarkers. For the initiation of the therapy, the most important markers are HBeAg, alanine-aminotranferase and HBV DNA serum levels and the viral genotype. During therapy, quantitative monitoring of the HBV DNA and HbsAg levels helps most to differentiate between those who will respond fast, those who will need longer treatment and those who are unlikely to respond and therefore need alternative drug therapy. A rapid decrease in or disappearance of HBsAg is predictive of successful response during interferon therapy, while changes in the HBV DNA level are more informative during treatment with nucleoside analogues. It is expected that information on the effects of combination therapy will become available soon and this may alter both the currently recommended treatment and monitoring strategy. Orv. Hetil., 2011, 152, 866–868.
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Ngemu, Erastus K., Christopher Khayeka–Wandabwa, Eliningaya J. Kweka, Joseph K. Choge, Edward Anino, and Elijah Oyoo-Okoth. "Retraction: effectiveness of option B highly active antiretroviral therapy (HAART) prevention of mother-to-child transmission (PMTCT) in pregnant HIV women." BMC Research Notes 7, no. 1 (2014): 868. http://dx.doi.org/10.1186/1756-0500-7-868.
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Shivaprakash, H. S., Manorama Tripathy, J. Sreenivasamurthy, and Manu V. Devadevan. "Book Reviews." Indian Theatre Journal 3, no. 1 (December 1, 2019): 57–65. http://dx.doi.org/10.1386/itj_00006_5.
Повний текст джерелаАнотація:
Kannada Theatre History 1850–1950: A Sourcebook, K. V. Akshara (ed.), B. R. Venkataramana Aithala and Deepa Ganesh (2018)Manipal: Manipal University Press, 288 pp.,ISBN 978-93-82460-84-8, p/bk, Rs. 250Theatre of the Earth – The Works of Heisnam Kanhailal: Essays and Interviews, Heisnam Kanhailal (2016)Calcutta: Seagull Books, 235 pp.,ISBN 978-8-17046-353-5, p/bk, Rs. 450Shakespeare: Kannada Spandana (in Kannada), Nataraj Huliyar (ed.) (2016)Bangalore: Kuvempu Bhasha Bharati Pradhikara, 330 pp.,ISBN 555-1-23408-868-3, p/bk, Rs. 150Chathirangam (in Malayalam), Ashok D’Cruz (ed.), C. K. Namboothiri (2018)Tirur: Thunchath Ezhuthachan Malayalam University, 256 pp.,ISBN 978-8-19374-580-9, h/bk, Rs. 250
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Amri, Nelly. "« L'homme de la terrasse » [La pratique religieuse et sociale d'un « ravi » en Dieu, le saint tunisois Ahmad b. `Arûs (m. 868/1463)]." Revue de l'histoire des religions 220, no. 4 (2003): 487–526. http://dx.doi.org/10.3406/rhr.2003.924.
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Razelos, Panagiotis. "Discussion: “Performance and Optimization Analysis for Fins of Straight Taper with Simultaneous Heat and Mass Transfer” (Kundu, B., and Das, P. K., 2004, ASME J. Heat Transfer, 126, pp. 862–868)." Journal of Heat Transfer 129, no. 9 (October 18, 2006): 1304–5. http://dx.doi.org/10.1115/1.2740643.
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Kasprowicz, L., G. Malloch, S. Foster, J. Pickup, J. Zhan, and B. Fenton. "Clonal turnover of MACE-carrying peach-potato aphids (Myzus persicae (Sulzer), Homoptera: Aphididae) colonizing Scotland." Bulletin of Entomological Research 98, no. 2 (December 13, 2007): 115–24. http://dx.doi.org/10.1017/s0007485307005445.
Повний текст джерелаАнотація:
AbstractPeach-potato aphids, Myzus persicae (Sulzer), collected in Scotland in the years 1995 and 2002–2004 were characterized using four microsatellite loci and three insecticide resistance mechanisms. From 868 samples, 14 multilocus genotypes were defined (designated clones A–N). Five of these (denoted A, B, H, M and N) carried modified acetylcholinesterase (MACE) resistance, the most recent resistance mechanism to have evolved in M. persicae. The current paper shows that the continued presence of MACE aphids is due to turnover, as clones A and B were replaced in field samples by clones H, M and N in later seasons. Thus, insecticide-resistant populations in Scotland can be attributed to multiple waves of rapid clone colonisations and not to the continued presence of stable resistant clones or mutation or sexual recombination in local populations. The MACE clones carried varying levels of the other insecticide resistance mechanisms, kdr and esterase. The presence of these mechanisms could alter the clones success in the field depending on insecticide spraying (positive selection) and resistance fitness costs (negative selection).
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Chi, Huanbin, Shoulin Wei, Feng Wang, and Zhongmu Li. "Identifying 46 New Open Cluster Candidates in Gaia EDR3 Using a Hybrid pyUPMASK and Random Forest Method." Astrophysical Journal Supplement Series 265, no. 1 (March 1, 2023): 20. http://dx.doi.org/10.3847/1538-4365/acb2cc.
Повний текст джерелаАнотація:
Abstract Open clusters (OCs) are regarded as tracers to understand stellar evolution theory and validate stellar models. In this study, we presented a robust approach to identifying OCs. A hybrid method consisting of pyUPMASK and the random forest (RF) algorithm is first used to remove field stars and determine more reliable members. An identification model based on the RF algorithm built based on 3714 OC samples from Gaia DR2 and EDR3 is then applied to identify OC candidates. The OC candidates are obtained after isochrone fitting, advanced stellar population synthesis model fitting, and visual inspection. Using the proposed approach, we revisited 868 candidates and preliminarily clustered them by the friends-of-friends algorithm in Gaia EDR3. Excluding OCs that have already been reported, we focused on the remaining 300 unknown candidates. From high to low fitting quality, these unrevealed candidates were further classified into Class A (59), Class B (21), and Class C (220). As a result, 46 new reliable OC candidates among Classes A and B are identified after visual inspection.
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Kozyrev, N. A., A. A. Usol’tsev, R. E. Kryukov, A. I. Gusev та I. V. Osetkovskii. "Operation indices of the new cored wire of Fe−C−Si−Мn−Сr−Ni−Mo system". Ferrous Metallurgy. Bulletin of Scientific , Technical and Economic Information 75, № 7 (8 серпня 2019): 860–68. http://dx.doi.org/10.32339/0135-5910-2019-7-860-868.
Повний текст джерелаАнотація:
To protect and repair details of equipment, subjected to abrasive and shock-and-abrasive wear, a build-up of hard alloy is widely used to increase the wear resistance of details, operating under abrasion conditions. Cored wires of Fe−C−Si−Мn−Сr−Ni−Mo system of type A and B by MIS classification are the main wires used for wear-resistant build-up in Russia. For effective application of building-up wires it is necessary to know the dependence of built-up layer hardness and its wear resistance on mass share of elements included into the composition of the cored wires systems. Influence of chemical composition of he built-up layer, obtained by the building-up with application of new powder systems of Fe−C−Si−Мn−Сr−Ni−Mo, protected by RF patents, on its physical and mechanical properties studied. Based on the results of the multifactor correlation analysis accomplished, dependences of the built-up layer hardness and its wear resistance on mass share of elements included into the composition of the cored wires of Fe−C−Si−Мn−Сr−Ni−Mo system determined. The dependences obtained were used for forecasting of the built-up layer hardness and its wear resistance at changes of the chemical composition of the built-up layer. The tests of JOY 4LS20 mining machine screw protective plates at Kemerovo region mines, built-up by the elaborated cored wire, showed an increase of the resistance by 19.3% comparing with the analog plates built-up by DRATEC wire.
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Ragnedda, Massimo, Maria Laura Ruiu, and Felice Addeo. "Measuring Digital Capital: An empirical investigation." New Media & Society 22, no. 5 (August 27, 2019): 793–816. http://dx.doi.org/10.1177/1461444819869604.
Повний текст джерелаАнотація:
This article develops a Digital Capital Index by adopting the definition provided by Ragnedda, who defines Digital Capital as the accumulation of digital competencies and digital technologies, and the model for measuring it developed by Ragnedda and Ruiu. It aims to develop a measure that can be replicated for comparison in different contexts. This article contributes both theoretically and empirically to the literature by (a) consolidating the concept of Digital Capital as a specific capital and (b) empirically measuring it. A Digital Capital Index is developed through an exploratory factor analysis (EFA) and validated with a representative sample survey of 868 UK citizens. The validation procedure shows that the Digital Capital Index is associated with socioeconomic and sociodemographic patterns, such as age, income, educational level and place of residence, while it appears not to be related to gender.
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Yamaguchi, Rumi, Masanobu Nakamura, Nobuyoshi Mochizuki, Steve A. Kay, and Akira Nagatani. "Light-dependent Translocation of a Phytochrome B-GFP Fusion Protein to the Nucleus in Transgenic Arabidopsis." Journal of Cell Biology 145, no. 3 (May 3, 1999): 437–45. http://dx.doi.org/10.1083/jcb.145.3.437.
Повний текст джерелаАнотація:
Phytochrome is a ubiquitous photoreceptor of plants and is encoded by a small multigene family. We have shown recently that a functional nuclear localization signal may reside within the COOH-terminal region of a major member of the family, phytochrome B (phyB) (Sakamoto, K., and A. Nagatani. 1996. Plant J. 10:859–868). In the present study, a fusion protein consisting of full-length phyB and the green fluorescent protein (GFP) was overexpressed in the phyB mutant of Arabidopsis to examine subcellular localization of phyB in intact tissues. The resulting transgenic lines exhibited pleiotropic phenotypes reported previously for phyB overexpressing plants, suggesting that the fusion protein is biologically active. Immunoblot analysis with anti-phyB and anti-GFP monoclonal antibodies confirmed that the fusion protein accumulated to high levels in these lines. Fluorescence microscopy of the seedlings revealed that the phyB-GFP fusion protein was localized to the nucleus in light grown tissues. Interestingly, the fusion protein formed speckles in the nucleus. Analysis of confocal optical sections confirmed that the speckles were distributed within the nucleus. In contrast, phyB-GFP fluorescence was observed throughout the cell in dark-grown seedlings. Therefore, phyB translocates to specific sites within the nucleus upon photoreceptor activation.
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Restall, M. "Magistrates of the Sacred: Priests and Parishioners in Eighteenth-Century Mexico. By William B. Taylor. Stanford, Calif.: Stanford University Press, 1996. 868 pp. $75.00." Journal of Church and State 39, no. 3 (June 1, 1997): 592–93. http://dx.doi.org/10.1093/jcs/39.3.592.
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Carro, M. D., S. Lopez, J. S. Gonzalez, and F. J. Ovejero. "The use of the rumen degradation characteristics of hay as predictors of its voluntary intake by sheep." Animal Science 52, no. 1 (February 1991): 133–39. http://dx.doi.org/10.1017/s0003356100005766.
Повний текст джерелаАнотація:
ABSTRACTEleven sun-cured hays with crude protein (CP) and neutral-detergent fibre (NDF) concentrations ranging from 57 to 207 and 428 to 744 g/kg dry matter (DM) respectively, were used to study the relationship between the DM and NDF degradation characteristics and the voluntary DM intake (VDMI) by sheep.The VDMI was measured in the last 10 days of a 21-day period using 50 mature ewes in five groups of 10 ewes per group, with a mean live weight (LAY) of 55 kg and ranged from 41·2 to 69·8 g/day per kg LW075. Disappearanc e rates of DM and NDF were measured by incubating samples in nylon bags in the rumens of three hay-fed rumen-cannulated ewes for 3, 6, 9, 15, 24, 48 and 72 h and by fitting the exponential model Y = a + b (1 — e-ct) to the results. Potential degradabilities (defined as a + b) for DM and NDF ranged from 679 to 868 and from 445 to 808 g/kg respectively.The VDMI was significantly correlated with the soluble fraction of DM (aDM; P < 0·05) and the rate of degradation of DM (cDM; P < 0·01) and NDF (cNDF; P < 0·01). The inclusion of aDM and cDM ni a multiple regression model resulted in the equation: VDMI = 21·3 + 0·0733 (s.e. 0·01577) aDM + 138 (s.e. 26·0) cDM (R2 = 0·897; residual s.d. = 2·79), with a significant increase of the variance explained by one single degradation characteristic (P < 0·05). The prediction was not improved by adding the constant cNDF to the equation (P > 0·05). These results indicate the possibility of predicting the VDMI of hays by sheep from the rumen soluble fraction (aDM) and the rate of degradation (cDM) of DM.
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Jing, Wenzhan, Siyu Zhao, Jue Liu, and Min Liu. "ABO blood groups and hepatitis B virus infection: a systematic review and meta-analysis." BMJ Open 10, no. 1 (January 2020): e034114. http://dx.doi.org/10.1136/bmjopen-2019-034114.
Повний текст джерелаАнотація:
ObjectiveHepatitis B virus (HBV) infection is a major public health problem worldwide. Several studies have reported that ABO blood groups may be associated with HBV infection. However, its association is still controversial. We performed a meta-analysis to investigate whether ABO blood groups were associated with HBV infection.DesignSystematic review and meta-analysis.Data sourcesRelevant studies available before 1 December 2019 were identified by searching PubMed, EMBASE, Web of Science, ScienceDirect and the Cochrane Library.Eligibility criteriaAll cross-sectional or cohort studies from which the data of ABO blood group distribution and HBV infection could be extracted.Data extraction and synthesisStudies were identified and extracted by two reviewers independently. Risk ratios (RRs) and 95% CIs were pooled by random-effect models to quantify this association.ResultsThirty-eight eligible articles including 241 868 HBV-infected subjects and 6 487 481 uninfected subjects were included. Overall, the risk of HBV infection had decreased by 8% in subjects with blood group B when compared with non-B blood group (RR=0.92, 95% CI 0.86 to 0.98). In the subgroup analyses, the inverse relationship between blood group B and HBV infection remained stable in higher endemic areas (HBV prevalence ≥5%), Asian people, larger sample size studies (≥2000), general population and blood donors, lower middle income group and studies published before the year 2010. Additionally, subjects with blood group O had a 12% increased risk of HBV infection (RR=1.12, 95% CI 1.01 to 1.24) in higher endemic areas. In the sensitivity analysis, the pooled risk estimates of blood group B and HBV infection were still stable.ConclusionsOur data suggested that the blood group B was associated with a lower risk of HBV infection. More research is needed to clarify the precise role of the ABO blood group in HBV infection to address the global question of HBV infection.
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Brito, Joscélia Monteiro Santos de, Gabriela Narezi, Paulo Dimas Rocha de Menezes, Rodrigo Antonio Ceschini Sussmann, Jaílson Santos de Novais, Emilly Da Silva Farias, Raquel Viana Quinelato, and Allison Gonçalves Silva. "Percepção ambiental quanto a qualidade da água utilizada na vila histórica de Caraíva, Porto Seguro – BA." Revista Brasileira de Geografia Física 14, no. 2 (April 22, 2021): 847. http://dx.doi.org/10.26848/rbgf.v14.2.p847-868.
Повний текст джерелаАнотація:
O saneamento ambiental é instrumento essencial para a manutenção da qualidade ambiental, bem-estar e saúde humana. O estudo da percepção ambiental pode fornecer instrumentos para superar impasses relacionados a essa temática, pois proporciona uma tomada de consciência das problemáticas ligadas ao ambiente vivido. Deste modo, o objetivo desse trabalho foi analisar a percepção ambiental dos moradores da vila histórica de Caraíva, Porto Seguro-BA quanto a qualidade da água utilizada, impactos na saúde, responsabilidades e expectativas para melhorias, na perspectiva de contribuir com informações que possam subsidiar ações que propiciem melhores condições de vida para a população local. Para isso, foram realizadas 77 entrevistas através de roteiro estruturado com questões abertas e fechadas e observações em campo entre novembro de 2019 e março de 2020. Os dados qualitativos foram interpretados e analisados através da técnica do Discurso do Sujeito Coletivo (DSC). Com auxílio do software estatístico RStudio, os dados foram submetidos ao teste qui-quadrado para verificar se há relação de dependência entre variáveis sociodemográficas e resultados qualitativos. Foi constatado que a população depende acentuadamente da água dos poços rasos individuais, sendo esta fonte mais utilizada que a encanada da rede pública. A insegurança quanto a qualidade da água reflete na alta adesão por água mineral envasada. A escolaridade foi o fator de maior peso quanto a percepção de que água imprópria possa colocar a saúde em risco. Os entrevistados reconhecem a responsabilidade do poder público tanto para a manutenção dos serviços de fornecimento da água, quanto pela conservação da sua qualidade. Enviromental perception regarding the quality of the water used in the historic village of Caraíva, Porto Seguro - BAA B S T R A C TEnvironmental sanitation represents an essential tool for maintaining environmental quality, well-being, and human health. The study of environmental perception, on the other hand, can provide instruments to overcome impasses related to this theme by providing an awareness of the problems related to the lived environment. This work aims to analyze the environmental perception of the historic village of Caraíva residents, in Porto Seguro, state of Bahia, Brazil regarding the quality of water used, health impacts, responsibilities, and expectations for improvements, It can also contribute with information to support actions that collaborate to provide better living conditions for the local population. For this purpose, 77 interviews were conducted through a structured script with open and closed questions and field observation between November 2019 and March 2020. Qualitative data were interpreted and through the technique of the Collective Subject Discourse (DSC). With the RStudio statistical software aid, the chi-square test was applied in the data to verify a dependency relationship between sociodemographic variables and qualitative results. According to the results, the population depends heavily on wells' water, and this water source is used more than a piped one. The insecurity regarding the water quality is reflected in the high adhesion to bottled mineral water. Schooling was the most significant factor in the perception that filtered water can put health at risk. Respondents recognize the public authorities' responsibility for maintaining water supply services and conserving their quality.Keywords: water resources management, environmental health, environmental impacts, environmental sanitation.
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Kiourti, Asimina, and Konstantina S. Nikita. "Design of Implantable Antennas for Medical Telemetry." International Journal of Monitoring and Surveillance Technologies Research 1, no. 1 (January 2013): 16–33. http://dx.doi.org/10.4018/ijmstr.2013010102.
Повний текст джерелаАнотація:
Implantable Medical Devices (IMDs) with wireless telemetry functionalities in the radio-frequency (RF) range are recently attracting significant scientific interest for medical prevention, diagnosis, and therapy. One of the most crucial challenges for IMDs is the design of the integrated implantable antenna which enables bidirectional wireless communication between the IMD and exterior monitoring/control equipment. In this paper, a parametric model of a miniature implantable antenna is initially proposed, which can be adjusted to suit any antenna design and implantation scenario requirements in hand. Dependence of the resonance, radiation, and safety performance of implantable antennas upon (a) operation frequency, (b) tissue anatomy and dielectric properties, and (c) implantation site is further studied. Simulations are carried out: (a) at 402, 433, 868 and 915 MHz considering a 13-tissue anatomical head model, (b) at 402 MHz considering five head models (3- and 5-layer spherical, 6-, 10- and 13-tissue anatomical) and seven dielectric parameter scenarios (variations ±20% in the reference permittivity and conductivity values), and (c) at 402 MHz considering 3-layer canonical models of the human head, arm, and trunk. The study provides valuable insight into the design of implantable antennas. Finite Element and Finite Difference Time Domain numerical solvers are used.
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Vidal, Jaime R. "Magistrates of the Sacred: Priests and Parishioners in Eighteenth-Century Mexico. By William B. Taylor. Stanford, Calif.: Stanford University Press, 1996. xiv + 868 pp. $75.00." Church History 66, no. 4 (December 1997): 849–50. http://dx.doi.org/10.2307/3169264.
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Dewbury, K. C. "Book reviewsManual of Ultrasound. By LutzH. and MeudtR., pp. 140, 1984 (Springer-Verlag, Berlin), DM58, $22.50. ISBN 3–540–12377–6." British Journal of Radiology 58, no. 693 (September 1985): 868. http://dx.doi.org/10.1259/0007-1285-58-693-868-b.
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Katamanova, Elena V., Natalya V. Efimova, Polina V. Kazakova, Oksana V. Ushakova, and Irina N. Kodinets. "Risk factors, quality of life and health of the elderly age persons." Hygiene and sanitation 100, no. 8 (August 31, 2021): 863–68. http://dx.doi.org/10.47470/0016-9900-2021-100-8-863-868.
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Introduction. Elderly people are among the most important social groups in Russian society, remaining the least protected, socially and biologically vulnerable part of the population. The objective of the study is to rank and assess the risk factors and health status of older people. Materials and Methods. 40 elderly persons aged 60 to 87 years were examined. The average age was 71.2 ± 7.4 years. Clinical, audiometric, stabilometric, psychological examination, biochemical blood test, osteodensitometry, ultrasound examination were carried out. An assessment of the health-related quality of life and diet is given. Statistica software was used for statistical processing. V.10 Results. On average, 5.3 diseases were found to be detected per one older person. This indicates the polymorbidity of the pathology. At the same time, more than half of the examined persons were found to have combined vascular lesions (52.5%) with lesions of the vessels of the brain and heart, accompanied by impaired cholesterol metabolism. The analysis of the indicators of the quality of life scales in the elderly population made it possible to establish pronounced problems associated with physical and emotional health and the deficit in the intake of B vitamins and protein in the diet. Conclusion. The older persons differ due to polymorbidity pathology. The analysis of the indicators of the quality of life scales in the elderly allowed establishing the presence of pronounced problems associated with physical and emotional health. It demonstrates the presence of increased depletion of mental activity and fatigue, and the lack of activation processes, which may further affect the limitation of daily activities and reduce the health-related life quality.
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Carvalho, F. T., F. A. R. Pereira, M. Peruchi, and R. R. B. Palazzo. "Manejo químico das plantas daninhas Euphorbia heterophylla e Bidens pilosa em sistema de plantio direto da cultura de soja." Planta Daninha 21, no. 1 (April 2003): 145–50. http://dx.doi.org/10.1590/s0100-83582003000100018.
Повний текст джерелаАнотація:
Objetivou-se neste trabalho avaliar a eficácia agronômica e os efeitos fitotóxicos de herbicidas aplicados em pré-plantio no manejo de Euphorbia heterophylla e Bidens pilosa, em sistema de plantio direto da cultura de soja. O experimento foi desenvolvido na safra 2000/2001, na FEP-UNESP, no município de Selvíria-MS. A variedade de soja utilizada foi a 'Conquista', e o delineamento experimental adotado foi o de blocos ao acaso, com sete tratamentos e quatro repetições, com oito linhas da cultura, totalizando 20 m² cada parcela. Os tratamentos foram os seguintes: glyphosate (720 e 960 g ha-1), chlorimuron-ethyl + glyphosate (10 g + 720 e 960 g ha-1), glyphosate + 2,4-D (960 + 868 g ha-1) e testemunhas no mato e no limpo. As aplicações foram realizadas com pulverizador de pressão constante (CO2) de 45 lb pol-2, com barra equipada com quatro bicos do tipo leque Teejet 110.03 XR, espaçados de 0,5 m, e volume de calda de 250 L ha-1. Concluiu-se que os tratamentos testados são eficientes na dessecação de E. heterophylla e B. pilosa e seletivos às plantas de soja. O herbicida chlorimuron-ethyl (10 g ha-1) aplicado juntamente com o glyphosate, no manejo, proporciona ao tratamento um efeito residual significativo para E. heterophylla e B. pilosa, reduzindo a infestação das plantas daninhas durante o ciclo da cultura da soja.
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Ito, Ryoma, Satoru Mizushiri, Yuki Nishiya, Shoma Ono, Ayumi Tamura, Kiho Hamaura, Akihide Terada, et al. "Two Distinct Groups Are Shown to Be at Risk of Diabetes by Means of a Cluster Analysis of Four Variables." Journal of Clinical Medicine 12, no. 3 (January 19, 2023): 810. http://dx.doi.org/10.3390/jcm12030810.
Повний текст джерелаАнотація:
Recent attempts to classify adult-onset diabetes using only six diabetes-related variables (GAD antibody, age at diagnosis, BMI, HbA1c, and homeostatic model assessment 2 estimates of b-cell function and insulin resistance (HOMA2-B and HOMA2-IR)) showed that diabetes can be classified into five clusters, of which four correspond to type 2 diabetes (T2DM). Here, we classified nondiabetic individuals to identify risk clusters for incident T2DM to facilitate the refinement of prevention strategies. Of the 1167 participants in the population-based Iwaki Health Promotion Project in 2014 (baseline), 868 nondiabetic individuals who attended at least once during 2015–2019 were included in a prospective study. A hierarchical cluster analysis was performed using four variables (BMI, HbA1c, and HOMA2 indices). Of the four clusters identified, cluster 1 (n = 103), labeled as “obese insulin resistant with sufficient compensatory insulin secretion”, and cluster 2 (n = 136), labeled as “low insulin secretion”, were found to be at risk of diabetes during the 5-year follow-up period: the multiple factor-adjusted HRs for clusters 1 and 2 were 14.7 and 53.1, respectively. Further, individuals in clusters 1and 2 could be accurately identified: the area under the ROC curves for clusters 1and 2 were 0.997 and 0.983, respectively. The risk of diabetes could be better assessed on the basis of the cluster that an individual belongs to.
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Wishart, G., D. Greenberg, J. Rashbass, P. Ravdin, G. Lawrence, C. Caldas, and P. Pharoah. "Adjuvant UK: Validation of a UK prognostic model for early breast cancer." Journal of Clinical Oncology 27, no. 15_suppl (May 20, 2009): 11086. http://dx.doi.org/10.1200/jco.2009.27.15_suppl.11086.
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11086 Background: To develop and validate a prognostication model to predict overall survival for women treated for early breast cancer in the UK based on cancer registry data. The main advantages are that unlike SEER, this dataset includes accurate information on mode of detection as well as systemic therapy. Methods: Using the Eastern Cancer Registration & Information Centre (ECRIC) dataset, information was collated for 5,818 women diagnosed with invasive breast cancer in East Anglia from 1999–2003. All patients underwent surgery, had records of pathological staging (tumour size, grade, lymph node status, ER status) and systemic treatment (chemotherapy, hormone therapy, both), mode of detection (screen-detected, symptomatic) and were followed up to December 2007. A model was derived from these data using Cox proportional hazards and this was subsequently validated in an external dataset of 5,468 patients from the West Midlands Cancer Intelligence Unit (WMCIU). Validation was performed by comparing (a) observed and predicted mortality - overall and by risk group decile - and (b) receiver operating characteristic (ROC) curves in both ECRIC & WMCIU datasets. Results: ECRIC dataset: Observed/predicted deaths were: 2 years (262/267), 5 years (868/867), 8 years (1108/1093). Area under ROC curve was 0.800WMCIU dataset: Observed/predicted deaths were: 2 years (317/298), 5 years (862/919), 8 years (955/999). Area under ROC curve was 0.783. The model and validation worked equally well at all time points (2, 5, 8 years) and in all risk group deciles. In the validation dataset the observed and predicted 8-year OS was within 1% (17.5% versus 18.3%). There was no significant statistical difference between the ROC curves (X2 = 2.35, P=.12) for prediction at 8 years in ECRIC and WMCIU data. Conclusions: We have developed a prognostication model for early breast cancer based on data from a UK cancer registry and validated the model using data from another UK registry. The model was well calibrated and provides a high degree of discrimination. This model will underpin the first web-based prognostication and treatment benefit tool for early breast cancer in the UK (Adjuvant UK). No significant financial relationships to disclose.
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Evans, R. Scott, Stanley L. Pestotnik, David C. Classen, Susan D. Horn, Sheron B. Bass, and John P. Burke. "Preventing Adverse Drug Events in Hospitalized Patients." Annals of Pharmacotherapy 28, no. 4 (April 1994): 523–27. http://dx.doi.org/10.1177/106002809402800417.
Повний текст джерелаАнотація:
OBJECTIVE: To use computerized adverse drug event (ADE) surveillance to help identify methods to reduce the number of ADEs in hospitalized patients. DESIGN: Prospective study of 79 719 hospitalized patients during a 44-month period. SETTING: LDS Hospital, a 520-bed tertiary care center affiliated with the University of Utah School of Medicine, Salt Lake City. INTERVENTION: Sequential study periods of at least one year each were compared. In the first period, data were collected but not reported to physicians, pharmacists, or nurses. In the subsequent study periods, three interventions (computerized alerts of drug allergies, standardized antibiotic administration rates, and timely physician notification of all ADEs) were made to reduce the number of type B (allergic or idiosyncratic reactions) and severe ADEs. RESULTS: In the first study period, we identified 56 type B ADEs during 120 213 patient days. During two subsequent study periods that included alerts to physicians of known drug allergies and standardized antibiotic administration rates, 8 type B events were identified during 113 237 patient days and 18 during 107 868 patient days, respectively (p<0.OO2). Early notification of physicians of all confirmed ADEs regardless of severity was associated with asignificant reduction of ADEs classified as severe from 41 during 113 859 patient days in the first study period to 12 during 103 071 patient days and 15 during 108 320 patient days in two subsequent study periods, respectively (p<0.00 1). CONCLUSIONS: Prospective surveillance of computer-based medical records for known drug allergies and appropriate drug administration rates can reduce the number of type B ADEs. Early ADE detection and notification of physicians permit drug and dosage changes that reduce the progression of mild and moderate ADEs to more severe conditions.
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Burkholder, Mark A. "Magistrates of the Sacred: Priests and Parishioners in Eighteenth-Century Mexico. By William B. Taylor. (Stanford: Stanford University Press, 1996. Pp. 868. Illustrations. Glossary. Notes. Bibliography. Index. $75.00.)." Americas 53, no. 4 (April 1997): 595–96. http://dx.doi.org/10.2307/1008154.
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Aguilar Ortega, Cecilia Beatriz, Julio Isaac Vega Cauich, and María José De Lille Quintal. "Desarrollo y validación de una escala de clima de seguridad organizacional." Ciencia Latina Revista Científica Multidisciplinar 7, no. 3 (July 13, 2023): 8164–82. http://dx.doi.org/10.37811/cl_rcm.v7i3.6805.
Повний текст джерелаАнотація:
El objetivo de este estudio fue desarrollar y validar una escala de clima de seguridad organizacional. Participaron 868 trabajadores pertenecientes a organizaciones del sector servicio y producción de México. Para la validación se siguieron los siguientes pasos: Fase 1. Diseño de la primera versión de la escala; Fase 2. Realización del Pilotaje; Fase 3. Aplicación de la escala para la validación y Fase 4. Validación de la escala. El análisis factorial señala que la escala quedo conformada por tres factores: b) Prioridad y acciones en seguridad; a) Participación en comisiones o reuniones sobre seguridad y c) Comunicación en prevención de riesgos. El análisis de confiabilidad arroja valores de .85 y .86 para el alfa y omega respectivamente. Finalmente, se brinda evidencia de validez señalando que la escala se asocia a un locus de control interno, hábitos positivos de sueño y una ingesta de alimentos saludables; y que permite diferenciar a trabajadores que han tenido un accidente laboral o que han sufrido un problema de salud asociado a su trabajo. Los resultados obtenidos son consistentes con la literatura previa y se concluye que existe evidencia de propiedades psicométricas que lo hacen un instrumento válido y confiable.
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Cindy Aura Briliana and Amsah Hendri Doni. "Analisis Pengaruh Pendapatan dan Pengetahuan terhadap Minat Berinvestasi di Pasar Modal Syariah pada Pedagang Grosir Pasar Aur Blok A,B & C Kota Bukittinggi." Transformasi: Journal of Economics and Business Management 3, no. 1 (April 3, 2024): 36–52. http://dx.doi.org/10.56444/transformasi.v3i1.1456.
Повний текст джерелаАнотація:
This research is motivated by the fact that Income and Knowledge are among the factors influencing Investment Interest, yet most traders have a relatively stable income from their trading businesses. The objective of this research is to determine and analyze the simultaneous and partial influence of income and knowledge on investment interest in the sharia capital market among wholesale traders in the Aur Market blocks A, B, and C in the city of Bukittinggi. This study is a field research using a descriptive quantitative method. The population in this study consists of 868 traders, and a sample of 90 traders is drawn to serve as respondents using a sampling method. The selection of traders is done randomly in the wholesale area of Aur Market blocks A, B, and C. Based on the data processing and analysis conducted, the result of the simple linear regression equation Y = 11,750 + 0.026 X1 + 0.449 X2 is obtained. It can be stated that Income (X1) has a positive but not significant influence, while Knowledge (X2) has a positive and significant influence on investment interest (Y) among wholesale traders in Aur Market blocks A, B, and C in the city of Bukittinggi. The result of the income value 0,202 < , meaning is accepted and is rejected, the knowledge value 5,241 > , meaning is rejected and is accepted. Simultaneously, the result of the income and knowledge 15,885 > , meaning is rejected and is accepted. The coefficient of determination ( ) result is 0.267 or 26.7%, which means that 26.7% of the investment interest is influenced by income and knowledge, while the remaining 73.3% is influenced by other variables not examined in this study. The theoretical independent variables are lifestyle, personality, and promotion.
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Kucukyilmaz, Ebru, Selcuk Savas, Gokhan Saygili, and Banu Uysal. "Evaluation of Apically Extruded Debris and Irrigant Produced by Different Nickel-Titanium Instrument Systems in Primary Teeth." Journal of Contemporary Dental Practice 16, no. 11 (2015): 864–68. http://dx.doi.org/10.5005/jp-journals-10024-1772.
Повний текст джерелаАнотація:
ABSTRACT Aim The purpose of this study was to determine the amount of extruded debris and irrigant associated with different single-file systems and one multiple-file system. Materials and methods Forty-five newly extracted single rooted primary canine teeth were used in this study. The root canals were instrumented using the reciprocating single-file system, the single-file rotary system and the multiple-file rotary system. A 10–6 precision micro-balance was used to calculate the amount of extruded debris and irrigant. The incubation period was set as 15 days at 37°C to obtain dry debris. The preparation time for instrumentation was also recorded. The data were statistically analyzed by Manova and Bonferroni adjustment. Results Considering the apically extruded debris, while there was statistically significant difference between Reciproc and OneShape groups (p < 0.05), no statistically significant difference was found among the other groups (p > 0.05). In terms of irrigation solution, although significant difference was obtained between the Reciproc and other two groups (p < 0.05), no statistically significant difference was obtained between the two rotary instruments (p > 0.05). Instrumentation was significantly faster using Reciproc than with all other instrument (p < 0.05). Conclusion All systems caused apical debris and irrigant extrusion. Full-sequences rotary instrumentation systems may be preferable for preparation primary teeth. Clinical significance This is the first study carrying out to compare both apical debris and irrigation solution extrusion with reciprocal and rotary single-file systems with other preparation systems in primary teeth. How to cite this article Kucukyilmaz E, Savas S, Saygili G, Uysal B. Evaluation of Apically Extruded Debris and Irrigant Produced by Different Nickel-Titanium Instrument Systems in Primary Teeth. J Contemp Dent Pract 2015;16(11):864-868.
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Sack, Brandon, Babak Moghimi, Barry Byrne, and Roland W. Herzog. "Anti-CD20 to Control Antibody Formation Against FVIII In Gene and Protein Replacement Therapy." Blood 116, no. 21 (November 19, 2010): 711. http://dx.doi.org/10.1182/blood.v116.21.711.711.
Повний текст джерелаАнотація:
Abstract Abstract 711 Neutralizing antibodies to FVIII (“inhibitors”) following delivery of exogenous clotting factor represents a major complication in the treatment of hemophilia A. Current treatment via immune tolerance induction (ITI) is effective but expensive, protracted, and imprecise. Recently, the drug rituximab—an anti-CD20 antibody used to deplete B cells—has been used with some success to treat existing inhibitors in patients. However, the potential for this drug to prevent inhibitor development has not been addressed. We used an IgG2a monoclonal anti-murine CD20 (kindly provided by Biogen Idec) to investigate the effect of B cell depletion on the immune response to human FVIII in hemophilia A mice (exon 16 deletion, C57BL6/129) and to determine the possibility of using B cell depletion to augment gene therapy. To deplete CD20+ B cells, mice were given two i.v. doses of 10mg/kg anti-CD20 (αCD20) two weeks apart. Depletion of peripheral blood B cells was confirmed by flow cytometry of blood, and depletion of lymph node and splenic B cells was tracked in control animals given the same regimen. Following administration of a single treatment of αCD20, peripheral blood B cells were depleted to <1% of total lymphocytes compared to 10–30% in control animals treated with isotype control antibody. One group of these mice (n=5, “αCD20+AAV8”) was given 1011vg/mouse of an AAV8 vector expressing hFVIII under a liver-specific promoter (AAV8-FVIII) one week after the first αCD20 injection. Another group (n=5, “AAV8-only”) was given AAV8-FVIII but not αCD20. Ten weeks after AAV8-FVIII treatment (8 weeks after last αCD20 treatment), both AAV8-only and αCD20+AAV8 groups were “challenged” with weekly i.v. injections of hFVIII at 1 IU/mouse for 4 weeks. Animals treated with αCD20+AAV8 showed somewhat better improvements in clotting times compared with animals treated only with AAV8, albeit that no anti-FVIII was detected in either group. However, the two groups differed more dramatically in their response to subsequent FVIII challenge. Mice in the AAV8-only group developed a mean anti-FVIII IgG1 titer of 7001 (±867) ng/mL and an average Bethesda titer of 336 (±88) BU, similar to the anti-hFVIII response seen normally in mice of this strain. In contrast, mice given αCD20+AAV8 were hypo-responsive with an anti-FVIII IgG1 titer of 1609 (±868) ng/mL and 22 (±11) BU. A third group of mice (n=5, “αCD20-only”) did not receive gene transfer and were challenged similarly, starting at 4 weeks after the last αCD20 treatment, i.e. when peripheral B cells were rebounding. Two weeks later, only 1 of 5 mice had a detectable antibody response with an IgG1 titer of 513 ng/mL and a Bethesda titer of 3.6 BU. Seven weeks after the end of the initial challenge, two of these mice were again challenged with another 4-week FVIII injection cycle. One animal again had no detectable anti-FVIII, while the other had 3478 ng/mL anti-FVIII IgG1 and 126 BU, still below the normal response. Together, these data suggest that antigen exposure upon transient depletion of B cells with anti-CD20 induces significant hypo-responsiveness to hFVIII. We have now generated human CD20 transgenic hemophilia A mice to test Rituximab for this purpose. Disclosures: Herzog: Genzyme Corp: Patents & Royalties.
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Gasbarro, Vincenzo, Luca Traina, Francesco Mascoli, Vincenzo Coscia, Gianluca Buffone, Raffaele Grande, Francesco Fugetto, Lucia Butrico, Stefano de Franciscis, and Raffaele Serra. "Absorbable suture material in carotid surgery." Vasa 44, no. 6 (November 2015): 451–57. http://dx.doi.org/10.1024/0301-1526/a000468.
Повний текст джерелаАнотація:
Abstract. Background: Absorbable sutures are not generally accepted by most vascular surgeons for the fear of breakage of the suture line and the risk of aneurysmal formation, except in cases of paediatric surgery or in case of infections. Aim of this study is to provide evidence of safety and efficacy of the use of absorbable suture materials in carotid surgery. Patients and methods: In an 11 year period, 1126 patients (659 male [58.5 %], 467 female [41.5 %], median age 72) underwent carotid endarterectomy for carotid stenosis by either conventional with primary closure (cCEA) or eversion (eCEA) techniques. Patients were randomised into two groups according to the type of suture material used. In Group A, absorbable suture material (polyglycolic acid) was used and in Group B non-absorbable suture material (polypropylene) was used. Primary end-point was to compare severe restenosis and aneurysmal formation rates between the two groups of patients. For statistical analysis only cases with a minimum period of follow-up of 12 months were considered. Results: A total of 868 surgical procedures were considered for data analysis. Median follow-up was 6 years (range 1-10 years). The rate of postoperative complications was better for group A for both cCEA and eCEA procedures: 3.5 % and 2.0 % for group A, respectively, and 11.8 % and 12.9 % for group B, respectively. Conclusions: In carotid surgery, the use of absorbable suture material seems to be safe and effective and with a general lower complications rate compared to the use of non-absorbable materials.
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Permatadewa, Elizza, and Taufan Asrisyah Ode. "HUBUNGAN CYBERSEX DENGAN PERILAKU SEKSUAL PRANIKAH PADA SISWA DI SMP NEGERI X KABUPATEN BANYUWANGI." IKESMA 19, no. 2 (June 25, 2023): 80. http://dx.doi.org/10.19184/ikesma.v19i2.38849.
Повний текст джерелаАнотація:
Remaja merupakan kelompok pengguna internet terbanyak di Indonesia. Banyak hal yang bisa dilakukan remaja ketika menggunakan internet, termasuk cybersex. Cybersex didefinisikan sebagai penggunaan internet dengan tujuan melakukan berbagai aktivitas untuk memuaskan seksual seperti melihat gambar porno, melakukan obrolan seksual, dan sebagainya. Cybersex merupakan salah satu fenomena yang berkembang dalam masyarakat modern di seluruh dunia. Pada Saat ini, fenomena tersebut telah menjadi masalah yang penting di masyarakat, terutama di kalangan generasi muda, khususnya remaja. Hal ini sangat erat hubungannya dengan perilaku seksual pranikah. Tujuan dari penelitian ini adalah untuk menganalisis hubungan antara cybersex dengan perilaku seksual pranikah pada siswa SMP Negeri X Kabupaten Banyuwangi. Jenis penelitian ini adalah kuantitatif dengan pendekatan cross sectional. Populasi penelitian ini adalah seluruh siswa SMP Negeri X Kabupaten Banyuwangi yang merupakan siswa aktif dengan jumlah 868 siswa dan jumlah sampel sebanyak 142 responden. Analisis yang digunakan dalam penelitian ini adalah univariat dan bivariat dengan menggunakan uji korelasi Kendalls tau-b. Hasil penelitian menunjukkan sebagian besar responden berjenis kelamin laki-laki (53,5%), mayoritas responden berada pada kategori remaja menengah 13–15 tahun (97,9%), sebagian besar responden melakukan cybersex pada kategori risiko rendah (84,5%) , dan perilaku seksual pranikah responden sebagian besar tergolong tidak berisiko (45,1%). Hasil uji korelasi Kendalls tau-b diperoleh nilai p (0,000), sehingga dapat disimpulkan bahwa ada hubungan antara cybersex dengan perilaku seksual pranikah pada siswa SMP Negeri X Kabupaten Banyuwangi. Berdasarkan hasil Penelitian ini disarankan agar orang tua dan guru memberikan pendidikan tentang seksualitas untuk mencegah siswa SMP melakukan cybersex dan perilaku seksual pranikah.
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Vijenthira, Abi, Lee Mozessohn, Chenthila Nagamuthu, Ning Liu, Danielle Blunt, Shabbir Alibhai, Anca Prica, and Matthew C. Cheung. "Frailty in Patients With Newly Diagnosed Diffuse Large B-Cell Lymphoma Receiving Curative-Intent Therapy: A Population-Based Study." Journal of the National Comprehensive Cancer Network 20, no. 6 (June 2022): 635–42. http://dx.doi.org/10.6004/jnccn.2022.7014.
Повний текст джерелаАнотація:
Background: The objectives of this study were to determine whether frailty is associated with survival in a population-based sample of patients with diffuse large B-cell lymphoma (DLBCL) and to describe the healthcare utilization patterns of frail versus nonfrail patients during treatment. Methods: A retrospective cohort study was conducted using population-based data in Ontario, Canada. Patients aged ≥66 years diagnosed between 2006 and 2017 with DLBCL or transformed follicular lymphoma who received first-line curative-intent chemoimmunotherapy were included. Frailty was defined using a modified version of a generalizable frailty index developed for use with Ontario administrative data. Cox regression was performed to examine the association between frailty and 1-year mortality. Results: A total of 5,527 patients were included (median age, 75 years [interquartile range, 70–80 years]; 48% female), of whom 2,699 (49%) were classified as frail. Within 1 year of first-line treatment, 32% (n=868) of frail patients had died compared with 20% (n=553) of nonfrail patients (unadjusted hazard ratio, 1.8; 95% CI, 1.6–2.0; P<.0001). Frail patients had higher healthcare utilization during treatment, with most hospitalizations related to infection and/or lymphoma. In multivariable modeling controlling for age, inpatient diagnosis, number of chemoimmunotherapy cycles received, comorbidity burden, and healthcare utilization, frailty remained independently associated with 1-year mortality (adjusted hazard ratio, 1.5; 95% CI, 1.3–1.7; P<.0001). Conclusions: In a population-based sample of older adult patients with DLBCL receiving front-line curative-intent therapy, half were classified as frail, and their adjusted relative rate of death in the first year after starting treatment was 50% higher than that of nonfrail patients. Frailty seems to be associated with poor treatment tolerance and a higher likelihood of requiring acute hospital-based care.
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Archer, Christon I. "Magistrates of the Sacred:Priests and Parishoners in Eighteenth-Century Mexico, by William B. TaylorMagistrates of the Sacred:Priests and Parishoners in Eighteenth-Century Mexico, by William B. Taylor. Stanford, California, Stanford University Press, 1996. xiv, 868 pp. $75.00 U.S." Canadian Journal of History 34, no. 2 (August 1999): 313–15. http://dx.doi.org/10.3138/cjh.34.2.313.
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Cely, Javier Enrique, Elkin José Mendoza, John Jaime Sprockel, Luis Carlos Perez, Janeth Marcela Mateus, Sebastian Maldonado, and Juan José Diaztagle. "Risk Factors for Community-Acquired Acute Kidney Injury in Medical Patients: A Nested Case-Control Study." Blood Purification 49, no. 6 (2020): 677–84. http://dx.doi.org/10.1159/000506502.
Повний текст джерелаАнотація:
<b><i>Introduction:</i></b> Research about the risk factors associated with community-acquired acute kidney injury (CA-AKI) in acute medical diseases is scarce. Data extrapolation from surgical to medical illnesses is questionable. <b><i>Objectives:</i></b> To evaluate potential risk factors and hospital outcomes associated with a CA-AKI in medical illnesses. <b><i>Methods:</i></b> We performed an unmatched nested case-control study from a previous prospective cohort study. We included adult patients with acute illnesses treated with internal medicine. Cases were defined as patients with a CA-AKI diagnosis upon hospital admission, and controls included patients from the same cohort who did not develop AKI during the first 5 days of hospitalisation. A logistic regression model was used to assess the association between potential risk factors and CA-AKI. <b><i>Results:</i></b> A total of 868 patients were included in the study (223 cases and 645 controls). The median age was 65 years (interquartile range 50–78). In a logistic regression model, the risk factors associated with CA-AKI included chronic kidney disease (CKD; OR 6.27; 95% CI 2.95–13.3, <i>p</i> < 0.001), ≥65 years old (OR 1.72; 95% CI 1.16–2.57, <i>p</i> = 0.007), acute bacterial infection (OR 1.95; 95% CI 1.36–2.80, <i>p</i> < 0.001), hypovolaemia (OR 1.88; 95% CI 1.32–2.69, <i>p</i> < 0.001), pre-hospital nephrotoxic drugs (OR 1.77; 95% CI 1.23–2.55, <i>p</i> = 0.002), anaemia (OR 1.49; 95% CI 1.03–2.14, <i>p</i> = 0.031) and systolic blood pressure (SBP) <107 mm Hg (OR 2.25; 95% CI 1.38–3.67, <i>p</i> = 0.001). A significant interaction between CKD and age was found (<i>p</i> = 0.017) and included in the model (patients with CKD and ≥65 years old [OR 10.85; 95% CI 4.14–28.41, <i>p</i> < 0.001]). The area under the receiver operating characteristic curve of the final model was 0.743. <b><i>Conclusions:</i></b> CKD is strongly associated with CA-AKI upon hospital admission in medical illnesses patients. Older age enhances the risk of CA-AKI in patients with CKD. Other risk factors include pre-hospital nephrotoxic drugs, acute bacterial infection, anaemia, low SBP and hypovolaemia.
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Radić, Mislav, Hana Đogaš, Andrea Gelemanović, Slavica Jurić Petričević, Ivan Škopljanac, and Josipa Radić. "Pulmonary Ultrasonography in Systemic Sclerosis-Induced Interstitial Lung Disease—A Systematic Review and Meta-Analysis." Diagnostics 13, no. 8 (April 16, 2023): 1429. http://dx.doi.org/10.3390/diagnostics13081429.
Повний текст джерелаАнотація:
Background: The aim of the current systematic review was to summarize and evaluate the overall advantages of lung ultrasonography (LUS) examination using high-resolution computed tomography (HRCT) as a reference standard in assessing the presence of interstitial lung disease (ILD) in systemic sclerosis (SSc) patients. Methods: Databases PubMed, Scopus, and Web of Science were searched for studies evaluating LUSs in ILD assessments including SSc patients on 1 February 2023. In assessing risk of bias and applicability, the Revised Tool for the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) was used. A meta-analysis was performed and the mean specificity, sensitivity, and diagnostic odds ratio (DOR) with a 95% confidence interval (CI) were obtained. In addition, in a bivariate meta-analysis, the summary receiver operating characteristic (SROC) curve area was additionally calculated. Results: Nine studies with a total of 888 participants entered the meta-analysis. A meta-analysis was also performed without one study that used pleural irregularity to assess the diagnostic accuracy of LUSs using B-lines (with a total of 868 participants). Overall sensitivity and specificity did not differ significantly, with only the analysis of the B-lines having a specificity of 0.61 (95% CI 0.44–0.85) and a sensitivity of 0.93 (95% CI 0.89–0.98). The diagnostic odds ratio of univariate analysis of the eight studies using the B-lines as a criterion for ILD diagnosis was 45.32 (95% CI 17.88–114.89). The AUC value of the SROC curve was 0.912 (and 0.917 in consideration of all nine studies), which indicates high sensitivity and a low false-positive rate for the majority of the included studies. Conclusions: LUS examination proved to be a valuable tool in discerning which SSc patients should receive additional HRCT scans to detect ILD and therefore reduces the doses of ionizing radiation exposure in SSc patients. However, further studies are needed to achieve consensus in scoring and the evaluation methodology of LUS examination.
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CAHILL, DAVID. "William B. Taylor, Magistrates of the Sacred: Priests and Parishioners in Eighteenth-Century Mexico (Stanford, CA: Stanford University Press, paperback ed. 1999), pp. xiv+868, £50.00, £19.95 pb; $75.00, $34.95 pb." Journal of Latin American Studies 32, no. 2 (May 2000): 549–601. http://dx.doi.org/10.1017/s0022216x00335842.
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Huffman, Fatma G., Joan A. Vaccaro, Gustavo G. Zarini, Daiane Biller, and Zisca Dixon. "Inadequacy of Micronutrients, Fat, and Fiber Consumption in the Diets of Haitian-, African- and Cuban-Americans with and without Type 2 Diabetes." International Journal for Vitamin and Nutrition Research 82, no. 4 (August 1, 2012): 275–87. http://dx.doi.org/10.1024/0300-9831/a000120.
Повний текст джерелаАнотація:
Micronutrient insufficiency, low dietary fiber, and high saturated fat intake have been associated with chronic diseases. Micronutrient insufficiencies may exacerbate poor health outcomes for persons with type 2 diabetes and minority status. We examined dietary intakes using the Recommended Dietary Allowances (RDAs) of micronutrients, and Adequate Intakes (AIs) of fiber, and Dietary Guidelines for Americans (DGA) for saturated fat in Haitian-, African-, and Cuban- Americans (n = 868), approximately half of each group with type 2 diabetes. Insufficient intakes of vitamins D and E and calcium were found in over 40 % of the participants. Over 50 % of African- and Cuban- Americans consumed over 10 % of calories from saturated fat. Haitian-Americans were more likely to have insufficiencies in iron, B-vitamins, and vitamins D and E, and less likely to have inadequate intake of saturated fat as compared to Cuban-Americans. Vitamin D insufficiency was more likely for Haitian-Americans as compared to African- Americans. Diabetes status alone did not predict micronutrient insufficiencies; however, Haitian-Americans with no diabetes were more likely to be insufficient in calcium. Adjusting for age, gender, energy, smoking, physical activity, access to health care, and education negated the majority of micronutrient insufficiency differences by ethnicity. These findings suggest that policies are needed to ensure that low-cost, quality produce can be accessed regardless of neighborhood and socioeconomic status.
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Reisman, D., and B. Sugden. "trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1." Molecular and Cellular Biology 6, no. 11 (November 1986): 3838–46. http://dx.doi.org/10.1128/mcb.6.11.3838-3846.1986.
Повний текст джерелаАнотація:
Two regions of the Epstein-Barr virus (EBV) genome together make up an element, oriP, which acts in cis to support plasmid replication in cells that express the EBV nuclear antigen 1 (EBNA-1). The two components of oriP are a region containing a 65-base-pair (bp) dyad symmetry and a region containing 20 copies of a 30-bp direct repeat. Here we show that the 30-bp family of repeats of oriP can function as a transcriptional enhancer that is activated in trans by the EBNA-1 gene product. In either EBV-genome-positive cells or in cells that express EBNA-1, the 30-bp family of repeats, when positioned in either orientation upstream or downstream, enhances expression of the chloramphenicol acetyltransferase (CAT) gene expressed from either the simian virus 40 early promoter or the herpes simplex virus type 1 thymidine kinase promoter. The extent of transcriptional enhancement varies with the promoter and cell type. This enhanced CAT expression reflects an increased level of CAT mRNA and does not result from amplification of the plasmids expressing CAT. In addition, plasmids carrying the gene for resistance to hygromycin B and the 30-bp family of repeats yielded 10 to 100 times more hygromycin B-resistant colonies than the vector lacking the 30-bp family of repeats in both EBV-genome-positive cells and cells that express EBNA-1. EBNA-1 is known to bind to sequences within the 30-bp family of repeats (D. R. Rawlins, G. Milman, S. D. Hayward, and G. S. Hayward, Cell 42:859-868, 1985), and these trans- and cis-acting elements together have at least two functional roles: (i) they are required for DNA replication dependent upon oriP, and (ii) they can enhance expression of genes linked to the 30-bp family of repeats of oriP.
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Natkunam, Yasodha, Gilad Vainer, Shuchun Zhao, Gail Amir, Eli Pikarsky, Anne S. Hammer, Stephen Hamilton-Dutoit, Ronald Levy, Joel K. Yisraeli, and Izidore S. Lossos. "RNA-Binding Protein VICKZ Is Expressed in a Germinal Center Associated Pattern among Lymphoma Subtypes." Blood 106, no. 11 (November 16, 2005): 1909. http://dx.doi.org/10.1182/blood.v106.11.1909.1909.
Повний текст джерелаАнотація:
Abstract Recent effort in the molecular characterization of diffuse large B-cell lymphoma (DLBCL) has led to the recognition that patients with DLBCL of germinal center origin exhibit a better overall survival. Thus, identification and characterization of markers of germinal center derivation are of importance in dissecting prognostic subclasses of DLBCL. The VICKZ (Vg1 RBP/Vera, IMP1, 2, 3, CRD-BP, KOC, ZBP-1) family members are RNA-binding proteins that recognize specific RNA targets and have been implicated in diverse cellular functions including cell polarity, migration, proliferation and tumorigenesis/metastasis. We generated an antibody that recognizes all three isoforms of VICKZ protein and characterized its expression in normal lymphoid tissue and in lymphoma subtypes. In normal tonsils, VICKZ protein showed a germinal center-specific pattern of expression with staining localized to the cytoplasm. Among 868 non-Hodgkin and Hodgkin lymphomas tested by immunohistochemistry on tissue microarrays, staining for VICKZ protein was present in 76% (126/165) of follicular lymphoma, 78% (155/200) of DLBCL, 90% (9/10) of mediastinal large B-cell lymphoma, and 100% (2/2) of Burkitt lymphoma. A subset of mantle cell lymphoma (11%, 2/19), extranodal (8%, 2/25), and nodal (20%, 1/5) marginal zone lymphoma and lymphoblastic lymphoma (25%, 4/13), showed VICKZ staining. The majority of lymphocyte predominant Hodgkin (92%, 12/13) and classical Hodgkin (94%, 101/108) lymphoma were found to be positive. Among T cell lymphoma, anaplastic large cell lymphoma were positive (75%, 6/9). Additional work is in progress to correlate VICKZ protein expression with other germinal center markers such as HGAL, BCL6 and CD10 as well as with prognostic subclasses of DLBCL. The differential expression pattern of VICKZ protein in lymphoma subtypes suggests a potential utility for VICKZ in the identification of subgroups of DLBCL associated with different prognoses.
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Reisman, D., and B. Sugden. "trans activation of an Epstein-Barr viral transcriptional enhancer by the Epstein-Barr viral nuclear antigen 1." Molecular and Cellular Biology 6, no. 11 (November 1986): 3838–46. http://dx.doi.org/10.1128/mcb.6.11.3838.
Повний текст джерелаАнотація:
Two regions of the Epstein-Barr virus (EBV) genome together make up an element, oriP, which acts in cis to support plasmid replication in cells that express the EBV nuclear antigen 1 (EBNA-1). The two components of oriP are a region containing a 65-base-pair (bp) dyad symmetry and a region containing 20 copies of a 30-bp direct repeat. Here we show that the 30-bp family of repeats of oriP can function as a transcriptional enhancer that is activated in trans by the EBNA-1 gene product. In either EBV-genome-positive cells or in cells that express EBNA-1, the 30-bp family of repeats, when positioned in either orientation upstream or downstream, enhances expression of the chloramphenicol acetyltransferase (CAT) gene expressed from either the simian virus 40 early promoter or the herpes simplex virus type 1 thymidine kinase promoter. The extent of transcriptional enhancement varies with the promoter and cell type. This enhanced CAT expression reflects an increased level of CAT mRNA and does not result from amplification of the plasmids expressing CAT. In addition, plasmids carrying the gene for resistance to hygromycin B and the 30-bp family of repeats yielded 10 to 100 times more hygromycin B-resistant colonies than the vector lacking the 30-bp family of repeats in both EBV-genome-positive cells and cells that express EBNA-1. EBNA-1 is known to bind to sequences within the 30-bp family of repeats (D. R. Rawlins, G. Milman, S. D. Hayward, and G. S. Hayward, Cell 42:859-868, 1985), and these trans- and cis-acting elements together have at least two functional roles: (i) they are required for DNA replication dependent upon oriP, and (ii) they can enhance expression of genes linked to the 30-bp family of repeats of oriP.