Дисертації з теми "66.047.4/.5"
Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: 66.047.4/.5.
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 дисертацій для дослідження на тему "66.047.4/.5".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте дисертації для різних дисциплін та оформлюйте правильно вашу бібліографію.
1
Рожновський, Максим Олександрович. "Удосконалення роботи сушарки киплячого шару у виробництві таблетованого ампіциліну". Master's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/39550.
Повний текст джерелаАнотація:
Пояснювальна записка до дипломного проекту «Удосконалення роботи сушарки киплячого шару у виробництві таблетованого ампіциліну» містить 92 сторінок, 39 літературних джерел, 21 рисунків, 23 таблиць.
В магістерській дисертації розглянуто сушарку з псевдозрідженим шаром та удосконалену частину апарату решітку сушарки псевдозрідженого шару. Решітка сушарки псевдозрідженого шару належить до галузі сушильних апаратів, які застосовують у лініях виробництва харчових продуктів та фармацевтичній галузі.
Виробництво твердих лікарських засобів (ЛЗ) є найбільш поширеною і відомою технологією у фармації, в якій на першому місці за кількістю продукції знаходяться таблетки.
Базовою вимогою до готової продукції будь-якого виробництва ЛЗ є забезпечення якості, безпечності та ефективності. Забезпечення цієї вимоги обумовлюється використанням обладнання з високою функціональною відповідністю чинній технології виробництва.
Пропонована решітка сушарки псевдозрідженого шару забезпечує якісне висушування матеріалу та підвищує ефективність роботи даної сушарки.
Для підтвердження працездатності та надійності конструкції були проведені тепловий, гідравлічний, конструктивний і міцнісний розрахунки елементів конструкції.
Проведений патентний пошук, за рахунок чого, виконаний порівняльний аналіз основних показників конструкції сушарки з діючими аналогами.
Також у проекті розглянуті питання з монтажу та експлуатації теплообмінника.
Розрахунки та креслення виконані згідно чинних стандартів, з використанням сучасних системних та інформаційних технологій.Explanatory note to the diploma project "Improvement of the fluidized bed dryer in the production of ampicillin tablets" contains 92 pages, 39 references, 21 figures, 23 tables. In the master's dissertation the dryer with a fluidized bed and the improved part of the apparatus of the lattice of the dryer of the fluidized bed are considered. The lattice of the fluidized bed dryer belongs to the field of dryers used in food production lines and the pharmaceutical industry. The production of solid drugs (DR) is the most common and well-known technology in pharmacy, in which the first place in terms of the number of products are tablets. The basic requirement for finished products of any drug production is to ensure quality, safety and efficiency. Ensuring this requirement is due to the use of equipment with high functional compliance with current production technology. The proposed lattice of the fluidized bed dryer provides high-quality drying of the material and increases the efficiency of this dryer. To confirm the efficiency and reliability of the structure, thermal, hydraulic, structural and strength calculations of structural elements were performed. A patent search was carried out, due to which a comparative analysis of the main indicators of the dryer design with the current analogues was performed. The project also addresses issues related to the installation and operation of the heat exchanger. Calculations and drawings are made according to current standards, using modem system and information technologies.
2
Westhoff, Timm Henning. "Pharmakologische Charakterisierung der vasoaktiven Eigenschaften von Adenosin(5')oligophospho-(5')guanosinen und Guanosin(5')oligophospho-(5')guanosinen." [S.l.] : [s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960608591.
Повний текст джерела
3
HANNIER, REGIS. "Les cardiomyopathies au 5 fluoro-uracile (5 fu)." Lille 2, 1990. http://www.theses.fr/1990LIL2M281.
Повний текст джерела
4
Rasmussen, Steven R. "Forecasting 5" /." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 1995. http://handle.dtic.mil/100.2/ADA304364.
Повний текст джерела
5
Harper, Jason Kenneth. "Yellow #5." Diss., Click here for available full-text of this thesis, 2006. http://library.wichita.edu/digitallibrary/etd/2006/t034.pdf.
Повний текст джерела
6
Duman, Isa. "Coke characterization on HZSM-5, Fe/ZSM-5, Ni/ZSM-5, and Fe-Ni/ZSM-5 from Catalytic Fast Pyrolysis of Biomass." Thesis, KTH, Skolan för kemi, bioteknologi och hälsa (CBH), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-226910.
Повний текст джерелаАнотація:
The combustion of fossil fuels has for a long time been a problem from an environmental and sustainability point of view, especially when it comes to the emissions of atmospheric carbon dioxide. The environmental concern has for instance shifted the attention towards finding new sustainable alternatives for producing chemicals and fuels, as a substitute to today’s dependence on fossil based crude oil. Catalytic Fast Pyrolysis of biomass is an excellent way to produce valuable chemicals and fuels using renewable resources. However, the process has some drawbacks, for example rapid deactivation of catalysts due to coke formation. Little is known about the characteristics of the formation of catalytic coke from pyrolysis processes, which should be a vital concern in future industrial processes. This thesis is dedicated to investigate the chemical coke characteristics found on zeolitic catalysts. Four zeolites of the type ZSM-5 were chosen for this thesis to deduce any chemical differences in the coke: HZSM-5, Fe/ZSM-5, Ni/ZSM-5, and Fe-Ni/ZSM-5. The coke were characterized by TGA, GC/MS, and FTIR. The results show that Fe/ZSM-5 produced the highest amount of coke compared to the other zeolites, where HZSM-5 had the lowest amount of coke formation. The coke consisted mainly of aromatic and cyclic hydrocarbons, dominated by polycyclic aromatic hydrocarbons. The content of ketones and alcohols in the coke found on HZSM-5 was higher compared to the metal-doped zeolites, while the formation of naphthalenes was lower. The FTIR results also show that coke was mainly comprised of aromatic hydrocarbons. However, traces of alkanes and alkenes reveal that the coke may have a greater variety than the GC/MS analysis suggests. The results show interesting features when metals are introduced to the zeolitic structure, at least when it comes to coke formation. The metal-doping of zeolites certainly seems to alter the chemistry of the catalytic reactions, compared to the parent zeolite. The differences in the chemical characteristics found in the coke are certainly interesting, and it could mean that the chemistry of the bio-oil also varies depending on the metals chosen for the ZSM-5. The new properties that metals introduce to the parent catalyst may open up new possibilities in industrial catalytic processes, and allow industries to take more advantage of the great benefits that biomass has to offer.Förbränning av fossila bränslen har under lång tid utgjort ett problem ur miljö- och hållbarhetssynpunkt, i synnerhet gällande utsläppen av koldioxid. En större miljömedvetenhet har gett upphov till sökandet efter nya råvaror för att framställa bränslen och kemikalier, utan att förlita sig på fossil råolja. Katalytisk pyrolys av biomassa är ett utmärkt sätt att framställa värdefulla kemikalier från förnybara källor. Processen står dock inför en del tekniska utmaningar, bland annat en snabb deaktivering av använda katalysatorer genom koksning. Målet med detta examensarbete är att undersöka den kemiska sammansättningen av koks, som bildats på zeolitkatalysatorerna. Mer specifikt, att försöka undersöka huruvida den kemiska sammansättningen av koks skiljer sig mellan katalysatorn HZSM-5 och metalldopad HZSM-5. Fyra katalysatorer valdes för detta examensarbete, nämligen HZSM-5, Fe/ZSM-5, Ni/ZSM-5 och Fe-Ni/ZSM-5. Kokset har analyserats genom termogravimetrisk analys (TGA), gaskromatograf kopplad med en masspektrometer (GC/MS), samt Fourier-transform-infraröd-spektroskopi (FTIR). Resultaten visar att Fe/ZSM-5 bildade en större mängd koks jämfört med de andra zeoliterna, varpå HZSM-5 hade lägst halt koks. Utöver detta bestod kokset till största del av aromatiska- och cykliska kolväten, speciellt polycykliska aromatiska kolväten. Innehållet av ketoner och alkoholer i kokset var störst för HZSM-5, medan bildandet av naftalenföreningar ökade för de metalldopade zeoliterna. FTIR-analysen gav även upphov till signaler som är signifikanta för både alkaner och alkener. Därför kan det innebära att kokset innehar en större kemisk variation än vad GC/MS-analysen påvisade. Resultaten visar intressanta egenskaper hos metallmodifierade zeoliter, i synnerhet gällande koksbildning. Det verkar som att de metalldopade zeoliterna påverkar de katalytiska reaktionerna som sker i katalysatorn, jämfört med den obehandlade katalysatorn. Skillnaderna i den kemiska sammansättningen hos kokset för de olika katalysatorerna är definitivt intressant och kan indikera att det även kan föreligga skillnader i den kemiska sammansättningen hos bio-olja, beroende på vilken metall ZSM-5 har behandlas med. De nya egenskaperna som metaller bidrar med till ZSM-5 kan öppna upp nya möjligheter i industriella katalytiska processer, vilket även kan medföra att industrier bättre kan ta tillvara på de fantastiska egenskaper biomassa innehar.
7
Bálint, Zoltán. "Průvlekový transformátor proudu 25 kV, 400/5/5 A." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2009. http://www.nusl.cz/ntk/nusl-217917.
Повний текст джерелаАнотація:
The aim of this master’s thesis is the design of a double-core bushing-type current transformer. This appliance is intended for measuring and protection of high-voltage distribution systems of the internal type for the highest system voltage of 25 kV. The master’s thesis is divided into four parts. The first part describes the function of the current transformer. The next part deals with the analysis of the phasor diagram and errors of the current transformer. The major part of the thesis deals with the design of the protective and measuring core. The last part describes the 3D model of the bushing-type current transformer.
8
Scarborough, Janna L. "5 Deans in 5 Years: Chairs Leading Through Change." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/2652.
Повний текст джерелаАнотація:
The chair/dean relationship often has a direct impact on departmental success. What happens when the there is a change in deanship? The presenters will share their experience of having five deans in five years. Through case studies and dialogue, strategies for navigating dean transitions will be shared and examined.
9
RESSAYRE, CATHERINE. "Modelisation du comportement cinetique du 5'-deoxy-5-fluorouridine (5'dfur) : apports methodologiques en pharmacocinetique experimentale." Aix-Marseille 2, 1990. http://www.theses.fr/1990AIX22985.
Повний текст джерела
10
Lüttgen, Maria. "Serotonergic receptor subtypes in learning and memory : focus on 5-HT1A, 5-HT1B and 5-HT2A receptors /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-628-6148-4/.
Повний текст джерела
11
Hinschberger, Antoine. "Synthèse et étude physico-chimique de nouveaux ligands des récepteurs 5-HT4, 5-HT6 et 5-HT7." Caen, 2000. http://www.theses.fr/2000CAEN4043.
Повний текст джерела
12
Perrin, Laurette. "5 ans, 5 jours et 5 nuits : instantané d’une recherche-création sur la blanchité à Salvador (BA)." Thesis, Bourgogne Franche-Comté, 2020. http://www.theses.fr/2020UBFCC007.
Повний текст джерелаАнотація:
Les mots sont des performances. Les actes sont des performances. Une étude académique est déjà un acte performatif en soi. Comment, en tant que personne blanche européenne étudiant à Salvador (Bahia), puis-je participer aux luttes contre les injustices sociales dans une perspective de décolonisation des sciences ? La recherche-création permet de questionner les formes de production de savoirs. Grâce au langage du Soundpainting – langage de gestes créé par Walter Thomson (1974) permettant de composer en temps réel la recherche-création,au-delà de son oralité et de sa pluridisciplinarité, propose une approche des concepts identitaires (genres, sexualités, racisme, identités, etc.) qui considère la particularité de leurs mouvances, de leurs formes évolutives et subjectives. « Recherche-collaborative », cette recherche a évolué au contact des différents performers qui ont participé aux créations, comme aux divers publics rencontrés. Chacun.e a contribué à l’évolution de son objet de recherche : « la blanchité à Salvador ». La première partie de cette thèse propose une présentation du concept de blanchité, tentant de définir certaines caractéristiques récurrentes, tout en tenant compte des particularités liées à chaque contexte socioculturel, ici la France et le Brésil. La seconde partie, quant-à-elle, décrit les différentes étapes du travail de recherche ainsi que son évolution au fil des rencontresWords are performances. Acts are performances. An academic study is, infact, a performative act in itself. As a white european studying in Salvador (Bahia),can I take a stand against social injustice in a perspective of decolonization of science? Research-creation allows us to question the ways of knowledge production. Thanks to the Soundpainting language – language of gestures created by Walter Thomson (1974) which allow us composing in real time - the research creation,in addition to its orality and pluridisciplinarity, proposes an approach to the concepts of identity (gender, sexualities, racism, identity, etc.) which considers the particularity of their movings and their evolving and subjective forms. As a "collaborative research", this research has evolved in contact with several performers who participated in the creation, as well as the various audiences we met. Each one has contributed to the evolution of its research object, "the whiteness in Salvador." The first part of this thesis proposes an overview of the concept of whiteness, trying to define some recurring characteristics, considering the particularities related to each socio-cultural context, here in France and also in Brazil. The second part describes the different stages of the research project an dits evolution over the meetings
As palavras são performances. Os atos são performances. Um estudoacadêmico já é em si um ato performativo. Enquanto pessoa branca europeia estudando em Salvador (Bahia), posso participar na luta contra a injustiça socialnuma perspectiva de descolonização da ciência? A pesquisa-criação permite questionar as formas de produção de conhecimento. Com a linguagem Soundpainting - linguagem de gestos criado por Walter Thomson (1974) para compor em tempo real - a pesquisa-criação, além de sua oralidade e de su apluridisciplinaridade, propõe uma abordagem dos conceitos de identidade (degênero, sexualidades, racismo, identidade, etc.) que considera a particularidade de suas movências, suas formas evolutivas e subjetivas. "Pesquisa-colaborativa",esta pesquisa tem evoluído em contato com vários artistas que participaram dacriação, como os vários públicos encontrados. Cada um.a contribuiu com aevolução do seu objeto de pesquisa, "a branquitude em Salvador." A primeira parte desta tese propõe uma visão geral do conceito de branquitude, tentando definir algumas características recorrentes, tendo em vista as particularidades decada contexto sociocultural, aqui na França e no Brasil. A segunda parte descreveas diferentes fases do trabalho de pesquisa e sua evolução ao longo dosencontros
13
Raux, Elizabeth A. "Synthesis of Selective 5-HT6 and 5-HT7 Receptor Antagonists." Digital Archive @ GSU, 2010. http://digitalarchive.gsu.edu/chemistry_theses/32.
Повний текст джерелаАнотація:
The development of novel selective 5-HT6 and 5-HT7 receptor antagonists is an ever-growing area of interest among medicinal chemists. The potential of developing a therapeutic agent useful as an antipsychotic or antidepressant, as well as the possibility to develop a drug for Alzheimer’s disease and obesity has led to an increase in synthesis of possible lead compounds. The synthesis of unfused biheteroaryl derivatives is described within. The derivatives have been evaluated for binding affinity at 5-HT2A, 5-HT6 and 5-HT7 receptors. The most potent 5-HT6 receptor antagonists include a benzene ring, a hydrophobic group and a protonated nitrogen atom. The most potent and selective compound synthesized is 1-[3-butyl-5-(thienyl)phenyl]-4-methylpiperazine. The binding site of the 5-HT7 receptor is similar to that of the 5-HT6 receptor and the most selective and potent 5-HT7 receptor antagonist also contains a potonated nitrogen atom and a hydrophobic group. The difference in selectivity between the 5-HT6 and 5-HT7 receptor antagonists is the aromatic ring. The most potent 5-HT7 receptor antagonist synthesized contains a pyridine ring instead of benzene, as in the 5-HT6 receptor antagonist. The most potent and selective 5-HT7 receptor antagonist is 1-[4-(3-furyl)-6-methylpyridin-2-yl]-4-methylpiperazine. The need to increase selectivity for both 5-HT6 and 5-HT7 receptors has led to the synthesis of flexible-chain linked derivatives and the results are described within.
14
Willoughby, Stephen P. "2 Cor 5:1-5 intermediate state or resurrection body? /." Theological Research Exchange Network (TREN), 1989. http://www.tren.com.
Повний текст джерела
15
Plateau, Pierre. "Métabolisme de l'Ap₄A (diadénosine 5', 5'''-P¹,P⁴-tétraphosphate)." Paris 11, 1985. http://www.theses.fr/1985PA112377.
Повний текст джерелаАнотація:
Nous étudions dans ce travail les mécanismes régulant la concentration intracellulaire l'Ap₄A et d’autres dinucléosides polyphosphates apparentés. Nous cherchons aussi à élucider la fonction de ces nucléotides. Les principaux résultats se résument ainsi. 1) Importance du zinc dans la synthèse des dinucléosides tri- et tétraphosphates : le zinc stimule spécifiquement la synthèse de ces nucléotides par certaines aminoacyl-tARN synthétases, les mêmes dans toutes les espèces étudiées. Cette constance au cours de l’évolution plaide en faveur de l’importance du zinc dans le métabolisme de ces nucléotides. 2) Purification et caractérisation d’une Ap₄A en deux ADP, et l'Ap₄A hydrolase d’E. Coli : cette enzyme hydrolyse l'Ap₄A en deux APD, et l’Ap₃A en ADP+AMP. Elle est très fortement stimulée par les ions cobalt et manganèse. 3) Mesure des dinucléosides polyphosphates dans des cellules d’E. Coli et de drosophile : l’accumulation de ces nucléotides en réponse à un stress oxydant a été généralisée à un eucaryote supérieur, la drososphile. D’autre part, l’existence de dinucléosides polyphosphates dans des cellules d’E. Coli en phase exponentielle a été établie. Des expériences sur des cellules bactériennes synchronisées montrent cependant que la concentration intracellulaire de ces nucléotides est constante au cours du cycle cellulaire. Ceci est à l'opposé de ce qui a été décrit chez les eucaryotes. 4) Clônage du gène de l'Ap₄A hydrolase d’E. Coli : ce clônage a permis la construction de souches affectées dans leur concentration intracellulaire d’Ap₄A. Ces souches devraient être d’une grande utilité pour comprendre les rôles biologiques de ce nucléotide.
16
LINDQVIST, EMMA. "5-i-9." Thesis, Högskolan i Borås, Institutionen Textilhögskolan, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-17361.
Повний текст джерелаАнотація:
This bachelor degree work investigates fashion as a non-verbal form of communication by the use of prints. Prints in fashion design today are categorized as placed prints or all-over prints. This work explores new dimensions in the use of a placed print by allowing it to interact with garment and body. It strives to take the prints within fashion one step further then just been pure surface decorations. With the main inspiration in T-shirt print design, the aim is to investigate the relation between halftoned, placed prints and garment by trying to achieve a depth within the expression. The main focus in the process is to find and define the depth within the printed textile and then to be able to apply it on garments on the body. Through research in material transparency in combination with screen printing, the work strives for a 3D moiré effect while moving. The work is to be seen as a suggestion of principles for interaction between prints, garments and body and to open up for a more complex way of working with prints within fashion. The combination between the striking 3D prints and the sharp black and white halftones creates a dynamic line-up. The collection provides the viewer with recognisable graphic pictures and elements as well as more abstract prints through layering.In this work it is found a possible technique to allow a message in a print to be communicated stronger. Through layering the printed textiles a new dimension were added to the expression in the motives and garments when interacting with the body. The continuation of this could be to try this effect with different motives and messages to be told. An interesting point of view would be to start with the viewer or receiver rather then the designers personal development in the project. It could possibly be as simple as creating a traditional T-shirt print with the message to communicate. Another alternative could be to carry out the communication of the motive in a whole garment and in how different garment types meet each other.Program: Modedesignutbildningen
17
Newson, Michael J. F. "Investigation of the effects of combined 5-HT transporter/5-HT autoreceptor blockade on central 5-HT function." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.432368.
Повний текст джерела
18
Thillier, Yann. "Synthèse chimique sur support solide d'ARN 5'-triphosphates et 5'-coiffés." Thesis, Montpellier 2, 2012. http://www.theses.fr/2012MON20101/document.
Повний текст джерелаАнотація:
Les ARN 5'-triphosphates (TP) et 5'-coiffés sont des molécules très convoitées des biologistes pour des études structurales par cristallographie ou comme outils thérapeutiques. Actuellement, ces oligonucléotides sont produits dans de faibles quantités via des procédés enzymatiques dépendants de la nature du nucléoside situé à l'extrémité 5'. Ces travaux se sont donc inscrits dans l'enjeu majeur de mettre au point une méthode chimique sur support solide qui permette l'accès à ces ARN 5'-fonctionnalisés en quantités importantes et sans restriction de séquences.Ce manuscrit rapporte une nouvelle méthode efficace de synthèse sur support solide d'ARN 5'-TP à grande échelle indépendante du nucléotide situé à l'extrémité 5' ou de la longueur de la séquence ARN. De plus, cette stratégie a été étendue avec quelques modifications à la synthèse d'analogues enzymatiquement plus stables de type: ARN 5'-β,γ-méthylène-TP, -(α-P-thio)-TP et -(α-P-thio)-(β,γ-méthylène)-TP.Une deuxième partie présente la synthèse supportée et l'évaluation antivirale de courts adénylates 2-5A 5'-TP portant des groupements enzymolabiles de type acétalesters en position 3' du ribose. Une dernière partie est consacrée à l'élaboration de nouvelles stratégies pour la synthèse d'ARN 5-coiffés. La première approche est un procédé en deux étapes où la structure coiffe (Gppp) est ajoutée sur support solide suivie d'une N7- méthylation enzymatique en solution, alors que la seconde est une méthode plus directe entièrement chimique. Par ailleurs, le couplage chimique de la coiffe sur les ARN supportés nécessite l'emploi d'un acide de Lewis. Ainsi l'utilisation de chlorures métalliques « verts » produits à partir de métaux de transition extraits de la biomasse a été évaluée dans la réaction du couplage de la coiffe sur les ARN5'-triphosphates RNAand 5'-capped RNA are high valuable molecules which serve as important substrates for structural and mechanistic studies or drugs. To date, these oligonucléotides are produced by enzymatic process in low yields with a weak variability of the 5'-end. To overcome this bottleneck we aimed to develop a chemical access on solid-support of these 5'-functionalized RNA in great amount without any limitations in the RNA sequence.Here, a robust, reproducible, and scalable method for the solid-phase synthesis of 5′-triphosphate RNA is presented. Furthermore, this strategy was extended to produce enzymatic resistant analogs of the triphosphate counterpart like: 5'-β,γ-methylene-TP, -(α-P-thio)-TP et -(α-P-thio)-(β,γ-methylene)-TP RNA.In a second part, the solid-phase synthesis of short adenylates 2-5A 5'-TP bearing biolabile acetalester groups on nucleosides 3'-hydroxyls and their antiviral activities are described.Finally, we focused on developing two approaches for the production of 5'-capped RNA. One is a two-steps process which consists in the coupling of the cap structure on solid-support followed by an enzymatic N7-methylation in solution while the other one is a straightforward chemical strategy. Beside, the chemical coupling of the cap moiety on solid-supported RNA requires the use of Lewis acids. Then, the ability of « green » metal chlorides prepared from phytoextracted heavy metals to promote this reaction was studied
19
Буренкова, Е. В. "Макроинициаторы на основе 5-метил-5-гексен-2,4-дионата кобальта (ІІ)". Дис. канд. хім. наук, Одес. нац. ун-т им. И.И. Мечникова, 2009.
Знайти повний текст джерела
20
Gilmore, P. E. "Effects of 5-HT₂, 5-HT₃ and 5-HT₄ ligands in the rodent elevated zero maze of anxiety." Thesis, Queen's University Belfast, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.268918.
Повний текст джерела
21
Diouf, Ousmane. "Nouveaux ligands potentiels des recepteurs serotoninergiques 5-ht1a, 5-ht2 et/ou 5-ht3 : etudes conceptuelle, chimique et pharmacodynamique." LILLE 2, 1993. http://www.theses.fr/1993LIL2P259.
Повний текст джерела
22
Ball, Ann-Katrin [Verfasser], Dieter [Gutachter] Steinhilber, and Thorsten [Gutachter] Maier. "Charakterisierung und zelluläre Lokalisation der humanen 5-Lipoxygenase und deren Isoformen 5-LO∆13, 5-LO∆4 und 5-LOp12 / Ann-Katrin Ball ; Gutachter: Dieter Steinhilber, Thorsten Maier." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2018. http://d-nb.info/1156326567/34.
Повний текст джерела
23
Teff, Karen. "Relationships between tryptophan, diet, 5-hydroxytryptamine metabolism, 5-hydroxytryptamine function and behavior." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74369.
Повний текст джерелаАнотація:
The role of tryptophan availability in the regulation of 5-hydroxytryptamine (5HT) metabolism and function has been examined in rats and humans, by manipulation of dietary components. It was demonstrated that in the rat, protein and carbohydrate can alter 5HT levels in peripheral tissues as well as brain. A new method for the determination of 5HT in the cisternal cerebrospinal fluid (CSF) of the rat was developed and used as an index of functional 5HT, using pharmacological agents known to act on 5HT function. Using this technique, it was then shown that tryptophan administration potentiates the release of 5HT when the animals are aroused, and thus when their 5HT neurons are firing at high rates. However, the smaller changes in brain 5HT after ingestion of protein or carbohydrate did not lead to altered CSF 5HT. In humans, similar conclusions were drawn. The administration of an amino acid mixture deficient in tryptophan significantly altered macronutrient selection, a behavior thought to be mediated by lowered tryptophan availability and 5HT function. However, when protein or carbohydrate breakfasts were given, no effect on macronutrient selection was observed, suggesting that the physiological changes resulting from dietary intake were not affecting functional 5HT. The final study measured amine precursors and metabolites in human lumbar CSF after the administration of protein or carbohydrate breakfasts and supported this hypothesis. No effects on CSF tryptophan or 5-hydroxyindoleacetic acid, the major metabolite of 5HT, were observed. In conclusion, the results of these studies suggest that, though large changes in precursor availability can alter 5HT function, the effects of dietary intake on 5HT metabolism are not normally of sufficient magnitude to alter 5HT function in rats or humans, or brain 5HT metabolism in humans.
24
East, Simon Zachary. "5-htâ†6 and 5-HTâ†7 receptor gene expression in schizophrenia." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343531.
Повний текст джерела
25
Perez, Carla Cristina 1979. "Sintese de 2-amino-3, 5-diois (1-deoxi-5-hidroxi-esfingolipidios." [s.n.], 2008. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248457.
Повний текст джерелаАнотація:
Orientador: Luiz Carlos DiasDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica
Made available in DSpace on 2018-08-11T02:21:07Z (GMT). No. of bitstreams: 1 Perez_CarlaCristina_M.pdf: 3422418 bytes, checksum: ae937d7829a969d84f9544e0e41a8aa3 (MD5) Previous issue date: 2008
Resumo: Neste trabalho descrevemos a reação diastereosseletiva entre aldeidos quirais N-Boc-a-aminoaldeídos e uma aliltricloroestanana aquiral fornecendo 1,2-syn N-Boc-aminoálcoois em altas diastereosseletividades. A estereoquímica relativa 1,2-syn dos produtos de adição foi determinada após conversão dos alcoóis homoalílicos nas correspondentes N-Boc-oxazolidinonas seguido pela analise das constantes de acoplamento no espectro de RMN de H. O tratamento dos alcoóis homoalílicos com quantidade catalítica de OsO4 na presença de NaIO4 forneceu as correspondentes 4-N-Boc-amino-3-hidroxicetonas. É importante ressaltar que não havia metodologias na literatura descrevendo a obtenção de 4-N-Boc-amino-3-hidroxicetonas, sendo este um método eficiente para obtenção dessa classe de compostos. A redução diastereosseletiva das 4-N-Boc-amino-3,5-hidroxicetonas forneceu os aminodióis 1,3-syn e 1,3-anti, respectivamente. A estereoquímica relativa 1,3 foi determinada após a conversão dos aminodióis nos correspondentes isopropilideno acetais e posterior análise de RMN de C
Abstract: We wish to describe here the diastereoselective reaction between chiral N-Boc-a-amino aldehydes and achiral allyltrichlorostannanes leading to 1,2-syn-N-Boc-a-amino alcohols in high diastereoselectivies. The 1,2-syn relative stereochemistry was determined after conversion of homoallylic alcohols to the corresponding N-Boc-oxazolidinones followed by analyses of the coupling constants in the H NMR spectra. Treatment of the homoallylic alcohols with catalytic amounts of OsO4 in the presence of NaIO4 provided the corresponding 4-N-Boc-amino-3-hydroxy ketones. It is very important to point out that, to the best of our knowledge, there was no useful and general approach to 4-N-Boc-amino-3-hydroxy ketones described in the literarure and this methodology stands as the most efficient approach to this class of compounds. Diastereoselective reduction of the 4-N-Boc-amino-3-hydroxy ketones provided the 1,3-syn and 1,3-anti aminodiols, respectively. The 1,3 relative stereochemistry was determined after C-NMR analyses of the corresponding isopropylidene acetals
Mestrado
Quimica Organica
Mestre em Química
26
Huamán, René Negrón. "Uma introdução a supercordas em plano e AdS 5 XS 5 background." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/43/43134/tde-19112014-130002/.
Повний текст джерелаАнотація:
Apresentamos uma revisão dos elementos básicos do estudo da teoria clássica das supercordas em backgrounds planos e curvos, dando ênfase ao caso importante em que o background e a variedade AdS5 S5. Nós inclumos um estudo da corda bosonica para revisarmos alguns conceitos básicos da teoria de campos conforme em duas dimensões. Em seguida estudamos a teoria das supercordas em um espaço plano onde apresentamos uma introdução pedagógica ao formalismo de espinores puros. A ultima parte e dedicada a generalização da ação de Green-Schwarz para o caso de AdS5 S5 e uma apresentação do modelo sigma do formalismo de espinores puros no mesmo background.We present a review of the basic elements of the study of classical superstring theory in at and curved backgrounds, giving emphasis to the very important case of the AdS5S5 background. We include a study of the bosonic string to review some basic concepts of two dimensional conformal eld theory. We then move on to the superstring in at space where we present a pedagogical introduction to the pure spinor formalism of superstrings. The last part is devoted to the generalization of the Green-Schwarz action to AdS5 S5 and a presentation of the pure spinor sigma model in the same background.
27
Левковець, С. І., П. М. Фочук, А. О. Федорчук, Г. Л. Мирончук, Л. В. Піскач, О. М. Юрченко та О. В. Парасюк. "Структура і властивості сплавів Tl[3]PbBr[5]-Tl[3]Pbl[5]". Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/45865.
Повний текст джерела
28
Pereira, Patrícia. "5 ritmos de dança." Master's thesis, [s.n.], 2011. http://hdl.handle.net/10400.26/15653.
Повний текст джерела
29
Borg-Rosén, Mikael, and David Rasmussen. "Bordsbokningssystem i HTML 5." Thesis, Tekniska Högskolan, Högskolan i Jönköping, JTH, Data- och elektroteknik, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-19341.
Повний текст джерелаАнотація:
Det finns idag få system som ger en god överblick av en restaurangs bokningar och bordsplaceringar. Oftast används papper och penna som ställer till med problem när kunder avbokar, ändrar deras bokning eller helt enkelt inte kommer till restaurangen. Det är även svårt att avgöra huruvida restaurangen är fullbokad eller ej. Denna rapport behandlar utvecklingen av ett webbaserat bordsbokningssystem för restauranger på uppdrag av en restaurangägare i Jönköping. Systemet ska ge en grafisk överblick av en restaurangs bokningar och hur alla bord ska stå placerade under en kväll. Då systemet webbapplikationen ska ha flera användare, som använder olika enheter, krävs det att systemet ska vara plattformsoberoende. Med hjälp av HTML5 undersöks det huruvida det går att göra detta system offlinekompatibelt, då uppdragsgivaren önskar att systemet är åtkomligt då Internetuppkopplingen försvinner. Det undersöks även hur en applikation görs användarvänlig och lättmanövrerad samt hur kommunikationen mellan klient och server görs mest effektivt. Projektet är uppdelat i fyra delar; grafiskt användargränssnitt, webbapplikation, datahantering och serverkommunikation, som i sin tur är indelade i två kategorier; klient och server. De två kategorierna användes som en bas för hela projektets gång, från litteraturgenomgång och forskning till implementation. Den slutliga webbapplikationen blev ett plattformsoberoende och offlinekompatibelt bordsbokningssystem, där användaren får en grafisk överblick av en vald dags bokningar samt bordsplaceringar.
30
Middendorff, Rasmus. "Webbokning i TeleQ 5." Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-194962.
Повний текст джерелаАнотація:
The Uppsala based company Aurora Innovation AB develops a call center solution called TeleQ with customer callback scheduling that decreases customer waiting time and increases agent productivity. There is also a possibility for the customer to book a callback time on the web. During the development of the fifth (and latest) version of TeleQ the need for a redesigned web booking module has emerged. This project aims to develop a future proof concept for how web booking should be handled in TeleQ 5.
31
Bemme, Jens. "5. Bibliothekskongress in Leipzig." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-119111.
Повний текст джерелаАнотація:
Aus 25 Ländern kamen die 3.500 Teilnehmer nach Leipzig zum diesjährigen 5. Kongress Bibliothek & Information Deutschland, der zugleich der 102. Deutsche Bibliothekartag war. Das Motto lautete vom 11. bis 14. März „Wissenswelten neu gestalten“. Erstmals präsentierte sich die Türkei als Gastland mit ihren Bibliotheken.
Aus den sächsischen Bibliotheken berichteten Kolleginnen und Kollegen in zahlreichen Vorträgen und Podien von neuen Ideen, Projekten und Kooperationen.
32
Schulze, Norman. "AFS-Kerberos-5-Migration." [S.l. : s.n.], 1998. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB10324578.
Повний текст джерела
33
Hellsin, Beppe. "Inomhuslokalisering med Bluetooth 5." Thesis, Tekniska Högskolan, Högskolan i Jönköping, JTH, Datateknik och informatik, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-39330.
Повний текст джерела
34
Meier, Lori T. "Episode 5: Historical Thinking." Digital Commons @ East Tennessee State University, 2021. https://dc.etsu.edu/social-studies-education-oer/5.
Повний текст джерелаАнотація:
In this episode, we take a deeper look at the definitions and five standards for historical thinking in the elementary social studies classroom. What does it mean for young learners and teachers to think like a historian?https://dc.etsu.edu/social-studies-education-oer/1004/thumbnail.jpg
35
Price, Jamie, and Ryan A. Nivens. "K-5 Computation + Math." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/4737.
Повний текст джерела
36
Schön, Magnusson Charlotta. "Miljöundervisning i årskurs 5." Thesis, Malmö högskola, Lärarutbildningen (LUT), 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-28202.
Повний текст джерелаАнотація:
Syftet med studien är att undersöka hur väl elever når upp till målen för miljöundervisning i årskurs 5 (Skolverket, 2000) och hur de ser på människans påverkan på miljön. Detta gjordes genom intervjuer där eleverna tilläts prata fritt inom vissa förutbestämda områden. Frågeområdena var förståelse för begrepp inom miljöområdet och miljöfrågor som speglade mina två forskningsfrågor. Resultatet visar att endast få elever når upp till kursplanemålen eller har möjlighet att göra så i slutet av skolåret. De flesta har även svårt att se individens påverkan på miljön och tror mer på samhällelig aktion. En bidragande orsak till detta kan vara bristande kunskap om och/eller intresse för miljöfrågor från elevernas lärare. Även tidspressen i skolan kan bidra till problemet. Kanske bör undervisningen ske på ett annat sätt än vad som görs, på ett sätt som bättre passar situationen.
37
Hirschfeld, C. René, Wolfgang Borchert, and Marie-Luise Kaschnitz. "5 Chorlieder: (op. 16)." C. René Hirschfeld, 1986. https://slub.qucosa.de/id/qucosa%3A71554.
Повний текст джерелаАнотація:
5 engagierte, hochexpressive Lieder für 4- bis 6stimmigen gemischten Chor, zwei Lieder davon mit obligatem Klavier. Charakter und auch Art des Satzes sind durchaus unterschiedlich. Die Spanne reicht von einfacher Linie im Chor mit virtuosem Klavierpart als Kontrapunkt über einen aphoristisch kurzen Chorsatz und einem reinen Klangstück ohne Text bis zu einem bedrohlich sich steigernden Fugato. Es handelt sich dabei nicht um einen festen Zyklus, d.h. die Lieder können auch einzeln gesungen werden, wiewohl eine Gesamtaufführung in der angegebenen Reihenfolge natürlich erwünscht ist.
Die Texte stammen von Wolfgang Borchert und Marie-Luise Kaschnitz.:1. Versuch es - S/A/T/B/Piano
2. Obertöne - S/A/T/B
3. Intermezzo - S/MS/A/T/Bar/B
4. Weißnoch - S/MS/A/T/Bar/B
5. Du sollst nicht - S/MS/A/T/Bar/B/Piano
38
Stevenson, Paula Louise. "Role of 5-ht2c receptor density on behaviour in mice." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/5570.
Повний текст джерелаАнотація:
The neurotransmitters serotonin (5-HT) and dopamine (DA) play roles in eating disorders, mood disorders, such as depression and anxiety, and in the regulation of locomotion. The 5-HT2C receptor is one of fourteen 5-HT receptor subtypes that is expressed in regions of the brain including the hippocampus, amygdala, dorsal striatum, nucleus accumbens (NA) and substantia nigra, and is therefore implicated in behaviours and disorders associated with these regions. 5-HT has been shown to exert both a tonic and phasic inhibitory control, through the 5-HT2C receptor, on the firing rate and bursting activity of DA-containing neurones in the ventral tegmental area which enhances DA release in the NA and prefrontal cortex. In addition, the 5-HT2C receptor is under the control of a monophasic diurnal rhythm and is in a position to alter circadian regulation and behaviour due to its expression in the suprachiasmatic nucleus (the light entrainable oscillator (LEO)). It was hypothesised that elevating expression of the 5-HT2C receptor would have a detrimental effect on mood and cause hypolocomotion while reducing 5-HT2C receptor expression would improve mood, cause hyperphagia, obesity and hyperlocomotion. In order to investigate these hypotheses mouse models that either over- or under-expressed the 5-HT2C receptor were implemented. The 5-HT2C receptor expression pattern and levels were confirmed in all mouse lines. A behavioural phenotype of hypolocomotion and increased anxiety in the 5-HT2C receptor over-expressing mice and hyperphagia, obesity and hyperlocomotion in the 5-HT2C receptor under-expressing mice were found the latter is conistent with current literature. During backcrossing of the mouse lines onto the C57Bl/6 genetic background the abnormal behavioural phenotypes were lost suggesting that 5-HT2C receptor function is particulary sensitive to the genetic background on which it is being expressed. In response to altered expression levels of 5-HT2C receptor, compensatory alterations were found in the 5-HT system, with an inverse relationship existing between both the 5-HT1A receptor mRNA expression levels and 5-HT release in the hippocampus with the expression levels of the 5-HT2C receptor. Over-expression of 5-HT2C receptor appears to inhibit DA release in the cortex. The circadian experiments showed that under-expressing the 5-HT2C receptor did not alter the regulation of the food entrainable oscillator and there was a suggestion that the regulation of the LEO was affected. In summary, these results demonstrate that altered expression of 5-HT2C receptors results in abnormal behaviours consistent with its role in psychiatric disorders, but that the outcome is dependent on the genetic background.
39
Grant, Gail. "Characterization of 5-hydroxyeicosanoid dehydrogenase and the regulation of 5-oxo-ETE synthesis." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92210.
Повний текст джерелаАнотація:
Inflammation is associated with numerous diseases including asthma and cancer. 5-Oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), an arachidonic acid metabolite formed by the 5-lipoxygenase (5-LO) pathway, is a potent chemoattractant for inflammatory cells. 5-LO converts arachidonic acid to 5S-hydroperoxy-ETE, which is reduced to 5S-hydroxy-ETE (5-HETE) by a peroxidase. 5-HETE is then oxidized to 5-oxo-ETE by the microsomal enzyme 5-hydroxyeicosanoid dehydrogenase (5-HEDH), which requires NADP+, and is abundant in most inflammatory cells and at least some structural cells. Although biochemical studies have contributed to our understanding of the regulation of 5-HEDH activity, this enzyme has not been purified nor has its sequence been identified.Here we report that B cells contain 5-HEDH, and synthesize 5-oxo-ETE from exogenous arachidonic acid in the presence of calcium ionophore. H2O2 strongly stimulates 5-oxo-ETE production via activation of the glutathione redox pathway, resulting in rapid increases in GSSG and NADP+. Various cancer cell lines, including PC3 (prostate), A427 (lung), and MCF7 (breast) also possess 5-HEDH activity and H2O2 also promotes the rapid synthesis of 5-oxo-ETE in these cells. 5-Oxo-ETE synthesis from 5-HETE by cancer cell lines was stimulated over a longer period of time (24 h) by anti-cancer agents including docosahexaenoic acid, tamoxifen and MK886. Although PC3 cells have little 5-LO activity, coculture experiments revealed that they can utilize neutrophil-derived 5-HETE to synthesize 5-oxo-ETE by transcellular biosynthesis. A similar process could result in the synthesis of 5-oxo-ETE in tumors, which contain large numbers of inflammatory cells. These studies suggest that oxidative stress and cell death, which occur at inflammatory sites and in tumors, could induce the synthesis of 5-oxo-ETE, which could then act to prolong the inflammatory response.
Another goal was to purify and characterize 5-HEDH. We succeeded in partially purifying this enzyme (~20-fold) from microsomal fractions from neutrophils and monocytic cells by detergent solubilisation followed by DEAE Sepharose and Blue Sepharose chromatography. Tryptic digests of the purified protein fractions were separated by SDS-PAGE and analyzed by mass spectrometry. Several known dehydrogenases were identified, along with one potential candidate with no known substrate: dehydrogenase/reductase SDR family member 7 (DHRS7). Further studies will be required to determine whether this dehydrogenase is identical to 5-HEDH.
L'inflammation est associée à de nombreuses maladies dont l'asthme et le cancer. L'acide 5-oxo-6,8,11,14-eicosatetraenoique (5-oxo-ETE), un métabolite de l'acide arachidonique produit par la 5-lipoxygénase (5-LO), est un puissant chimioattractant pour les cellules du système inflammatoire. La 5-LO transforme l'acide arachidonique en 5S-hydroperoxy-ETE, qui est réduit en 5S-hydroxy-ETE (5-HETE) par une peroxydase. Le 5-HETE est oxydé en 5-oxo-ETE par une enzyme microsomale, la 5-hydroxyeicosanoide déshydrogénase (5-HEDH), enzyme dépendante du NADP+ et présente en abondance dans la majorité des cellules inflammatoires ainsi que dans quelques cellules structurales. Bien que les études biochimiques contribuent à une meilleur compréhension de la régulation de la 5-HEDH, cette enzyme n'a pas encore été purifiée ni clonée.
Dans cette étude, nous rapportons que les cellules B possèdent de la 5-HEDH, et produisent du 5-oxo-ETE à partir d'acide arachidonique exogène en présence d'ionophore de calcium. Le H2O2 stimule fortement la production de 5-oxo-ETE via l'activation du cycle de réduction du glutathion, provoquant une augmentation rapide du GSSG et du NADP+. Différentes lignées cellulaires cancéreuses, les PC3 (prostate), A427 (poumon), et MCF7 (sein), possèdent de la 5-HEDH et sont capables de synthétiser du 5-oxo-ETE après stimulation par le H2O2. Cette synthèse est augmentée suite à une exposition de 24h avec des agents anticancéreux : l'acide docosahenoique, tamoxifène et MK886. Bien que les PC3 n'aient pas beaucoup d'activité 5-LO, des expériences de coculture avec des neutrophiles démontrent qu'elles peuvent utiliser le 5-HETE produit par les neutrophiles pour synthétiser du 5-oxo-ETE par biosynthèse transcellulaire. Un processus similaire peut être à l'origine de la synthèse de 5-oxo-ETE dans les tumeurs, riches en cellules inflammatoires. Cette étude suggère que le stress oxydatif ainsi que la mort cellulaire, présents aux sites inflammatoires et dans les tumeurs, peuvent induire la synthèse du 5-oxo-ETE, qui par lui-même peut prolonger la réponse inflammatoire. fr
Dans cette étude, nous avons tenté de purifier et de caractériser la 5-HEDH. Nous avons réussi à la purifier partiellement à partir de microsomes, de neutrophiles et de cellules monocytique, solubilisés avec des détergents, suivit par chromatographie avec du DEAE sépharose et du sépharose bleu. Les différentes fractions obtenues ont été digérées par la trypsine, séparées sur gel acrylamide et analysées par spectrométrie de masse. Plusieurs déshydrogénases ont été identifiées, dont la déshydrogénase/réductase membre no. 7 de la famille SDR (DHRS7), qui ne possède pas de substrat connu. D'autres études devront être effectués pour déterminer si cette déshydrogénase correspond à 5-HEDH. fr
40
Charest, Alain. "Characterization of the 5'-flanking region of the rat 5- hydroxytryptamine1A receptor gene." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56964.
Повний текст джерелаАнотація:
To identify the genetic mechanisms underlying the cell specific expression displayed by the rat 5-hydroxytryptamine$ sb{ rm 1A}$ (5-HT$ sb{ rm 1A}$) receptor, over 3.2-Kb of 5$ sp prime$-flanking region were sequenced and characterized. A unique transcription initiation site was detected at -967 bp 5$ sp prime$ to the translation initiation codon (+1 bp) and was specific to hippocampal tissues which are known to strongly express 5-HT$ sb{ rm 1A}$ receptors. By sequence analysis, a TATA consensus sequence was identified 58 bp upstream to the transcription initiation site and several other cis-acting elements were also observed. To assess potential transcriptional activity, deletion fragments of the 3.2-Kb 5-HT$ sb{ rm 1A}$ receptor gene 5$ sp prime$ region were linked to a luciferase reporter gene and transiently expressed in mammalian cells (P19 and Ltk) which do not endogenously express the 5-HT$ sb{ rm 1A}$ receptor gene. Results indicated a strong promoter located between nucleotides -1187 and -117, followed by an upstream silencer region (-1390/-1188), the repressor activity of which is attenuated by the presence of additional upstream sequences. I suggest that repression of transcription may be a major mechanism restricting the expression of the 5-HT$ sb{ rm 1A}$ receptor gene to the appropriate tissues.
41
Cobb, L. J. "Roles of insulin-like growth factor binding protein-5 (IGFBP-5) during myogenesis." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.597793.
Повний текст джерелаАнотація:
Mutation of the N-terminal IGF-binding site of IGFBP-5 and its transfection into myoblasts revealed that the functions of IGFBP-5 in myogenesis can be divided in to IGF-dependent inhibition of myogenesis, and an IGF-independent anti-apoptotic role, assessed by decreased caspase-3 and PARP cleavage, caspase-3 and -9 activities, and decreased annexin V staining of cell populations overexpressing wild type (wtIGFBP-5) and non-IGF binding IGFBP-5 (mutIGFBP-5). Further analysis revealed the decreased apoptosis stimulated by wt and mutIGFBP-5 correlates with increased Bcl-XL protein, and Bad phosphorylation levels. Transfection of myoblasts with wtIGFBP-5 resulted in decreased Akt phosphorylation, increased p38 activation and slightly increased p21 protein levels. Closer examination of cells overexpressing mutIGFBP-5 revealed enhanced myogenesis, increased p38 activation, and slightly, but consistently, increased p21 levels. Intriguingly, increased Akt phosphorylation was also observed in mutIGFBP-5 overexpressing cells which occurred independently of type I receptor activation. This suggests that mutIGFBP-5 was activating an alternative pathway to enhance the myogenic programme. To study the influence of the secretory pathway on the functions of IGFBP-5, the signal peptide was removed (nsIGFBP-5). The overexpression of nsIGFBP-5 in C2 cells had little effect on the rate or extent of myogenesis, Akt phosphorylation or p38 activation when compared with control cells, but increased p21 levels to a greater extent than did wtIGFBP-5. However, more dead cells were visible. When apoptosis was examined in these cell populations, nsIGFBP-5 no longer had a protective effect, as assessed by annexin V staining and caspase-3. Indeed, cells overexpressing nsIGFBP-5 appeared to have slightly elevated apoptosis, and an intriguing 4-fold increase in caspase-9 activation, which was not translated fully in to an increase in caspase-3 activity. The slightly elevated caspase-3 activity interestingly appeared to correlate with an approximate 50% reduction in Bcl-2 protein levels.
42
Liu, Jue Judy. "Epigenetic shielding: 5-hydroxymethylcytosine and 5-carboxylcytosine modulate UV induction of DNA photoproducts." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11602.
Повний текст джерелаАнотація:
Maintaining the balance between dynamic DNA methylation and demethylation is crucial to mammalian development and pathogenesis. In vitro methylation at the C-5 position of cytosine enhances cyclobutane pyrimidine dimer (CPD) formation and promotes transition mutations. While the loss of 5-hydroxymethylcytosine (5hmC) and inactivation of the ten-eleven translocation (TET) family have been implicated in cancers, and repeated exposure to UV radiation is a known risk factor for developing skin cancers, the link between DNA demethylation and UV damage has not yet been illustrated. We report that hydroxylation and carboxylation of 5-methylcytosine mitigate methylation-induced CPD enhancement upon UV irradiation. However, 5hmC also increases UV induction of (6-4) photoproducts. In a melanoma cell model, this duality by 5hmC in modulating the UV response is accentuated through TET2 overexpression. These findings implicate the DNA demethylation intermediates 5-hydroxymethylcytosine (5hmC) and 5-carboxylcytosine (5caC) as selective epigenetic shields against UV induction of DNA photoproducts.
43
Fleury, Thiago Simonetti. "Harmonic superspace from the Ad'S IND. 5' × 'S POT. 5' pure spinor formalism /." São Paulo, 2013. http://hdl.handle.net/11449/108876.
Повний текст джерелаАнотація:
Orientador: Nathan Jacob BerkovitsBanca: Brenno Carlini Vallilo
Banca: Andrey Yuryevich Mikhaylov
Banca: Dmitry Melnikov
Banca: Ion Vasile Vancea
Resumo: A conjectura de Maldacena ou (AdS/CFT) desde a sua formulação é um dos tópicos em física de altas energias mais estudados. Uma das versões da conjectura é a dualidade entre a teoria de supercordas do tipo IIB em um background Ad'S IND. 5' × 'S POT. 5' suportado por um fluxo Ramond-Ramond e a teoria deN = 4 super-YangMills em quatro dimensões. Embora a ação para supercordas neste background seja conhecida tanto no formalismo de Green-Schwarz como no formalismo de espinores puros, a construção explícita dos operadores de vértice da teoria em termos de supercampos é um problema em aberto. Nesta tese, os operadores de vértice do formalismo de espinores puros correspondentes aos estados de supergravidade são construídos próximos a fronteira de AdS. A conjectura prevê que todo estado na camada de massa da supercorda é dual a um operador invariante de gauge de N = 4 d = 4 super-Yang-Mills, em particular, os estados de supergravidade são duais a operadores Half-BPS. Os operadores Half-BPS e seus duais podem ser descritos como supercampos em um superespaço harmônico. Os resultados obtidos para os operadores de vértice são descritos em função desses supercampos duais de acordo com o previsto pela conjectura
Abstract: The Maldacena's conjecture or (AdS/CFT) has been one of the most studied topics in high energy physics since its formulation. One of the versions of the conjecture is the duality between the theory of type IIB superstrings in the background Ad'S IND. 5' × 'S POT. 5' supported by a Ramond-Ramond flux and the theory of N = 4 superYang-Mills in four dimensions. Although the action for the superstrings in this background is known both in the Green-Schwarz and in the pure spinor formalisms, an explicit superfield construction of the vertex operators of the theory is an open problem. In this thesis, using the pure spinor formalism, we explicitly construct the vertex operators corresponding to supergravity states close to the boundary ofAdS. The conjecture predicts that every on-shell superstring state is dual to a gaugeinvariant operator of N = 4d = 4 super-Yang-Mills, in particular, the supergravity states are dual to Half-BPS operators. It is possible to describe all the Half-BPS operators and their duals as superfields in harmonic superspace. The results for the vertex operators are described in terms of these dual superfields in agreement with the prediction of the conjecture
Doutor
44
Fleury, Thiago Simonetti [UNESP]. "Harmonic superspace from the Ad'S IND. 5' × 'S POT. 5' pure spinor formalism." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/108876.
Повний текст джерелаАнотація:
Made available in DSpace on 2014-08-27T14:36:42Z (GMT). No. of bitstreams: 0
Previous issue date: 2013-09-17Bitstream added on 2014-08-27T15:57:10Z : No. of bitstreams: 1
000773092.pdf: 628332 bytes, checksum: eb30fdb970ce8b80e0cf4467a8bb5c64 (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
A conjectura de Maldacena ou (AdS/CFT) desde a sua formulação é um dos tópicos em física de altas energias mais estudados. Uma das versões da conjectura é a dualidade entre a teoria de supercordas do tipo IIB em um background Ad'S IND. 5' × 'S POT. 5' suportado por um ?uxo Ramond-Ramond e a teoria deN = 4 super-YangMills em quatro dimensões. Embora a ação para supercordas neste background seja conhecida tanto no formalismo de Green-Schwarz como no formalismo de espinores puros, a construção explícita dos operadores de vértice da teoria em termos de supercampos é um problema em aberto. Nesta tese, os operadores de vértice do formalismo de espinores puros correspondentes aos estados de supergravidade são construídos próximos a fronteira de AdS. A conjectura prevê que todo estado na camada de massa da supercorda é dual a um operador invariante de gauge de N = 4 d = 4 super-Yang-Mills, em particular, os estados de supergravidade são duais a operadores Half-BPS. Os operadores Half-BPS e seus duais podem ser descritos como supercampos em um superespaço harmônico. Os resultados obtidos para os operadores de vértice são descritos em função desses supercampos duais de acordo com o previsto pela conjectura
The Maldacena’s conjecture or (AdS/CFT) has been one of the most studied topics in high energy physics since its formulation. One of the versions of the conjecture is the duality between the theory of type IIB superstrings in the background Ad'S IND. 5' × 'S POT. 5' supported by a Ramond-Ramond ?ux and the theory of N = 4 superYang-Mills in four dimensions. Although the action for the superstrings in this background is known both in the Green-Schwarz and in the pure spinor formalisms, an explicit super?eld construction of the vertex operators of the theory is an open problem. In this thesis, using the pure spinor formalism, we explicitly construct the vertex operators corresponding to supergravity states close to the boundary ofAdS. The conjecture predicts that every on-shell superstring state is dual to a gaugeinvariant operator of N = 4d = 4 super-Yang-Mills, in particular, the supergravity states are dual to Half-BPS operators. It is possible to describe all the Half-BPS operators and their duals as super?elds in harmonic superspace. The results for the vertex operators are described in terms of these dual super?elds in agreement with the prediction of the conjecture
FAPESP: 09/50775-3
45
Brouard, Julia Therese. "Evidence for a 5-HT₆ receptor-mediated control of midbrain 5-HT neurons." Thesis, University of Oxford, 2016. https://ora.ox.ac.uk/objects/uuid:30a49d17-e976-4cc8-b718-392d725d2ea5.
Повний текст джерелаАнотація:
The dorsal raphe nucleus (DRN) contains the majority of 5-hydroxytryptamine (5-HT)-producing neurons, whose firing is influenced by homeostatic feedback pathways. Dysfunction of 5-HT neurons is implicated in the pathophysiology of diseases such as depression. Thus, pathways regulating 5-HT neuron activity represent potential therapeutic targets. Negative feedback pathways influencing 5-HT neurons include 5-HT1A autoreceptors located in the DRN, and feedback from 5-HT1A, 5-HT2A and 5-HT2C receptors on postsynaptic neurons in forebrain regions. One pathway, mediated by 5-HT4 receptors on medial prefrontal cortex (mPFC) neurons, positively regulates 5-HT neuron activity. Preliminary findings in the Sharp Laboratory suggested that 5-HT6 receptors may also positively regulate 5-HT neuron activity. Using in vivo electrophysiological recordings in combination with drug tools, this thesis investigated the 5-HT6 receptor control of 5-HT neurons, a proportion of which were identified as 5-HT-containing using a juxtacellular labelling technique. Electrophysiological recordings in anaesthetised rats found that 5-HT6 receptor agonists WAY-181187 and WAY-208466 increased 5-HT neuron firing to 58% and 47% above pre-drug firing, respectively. By contrast 5-HT6 receptor antagonist SB-399886 reduced 5-HT neuron firing to 56% below pre-drug firing. Four other 5-HT6 ligands, agonist ST-1936, and antagonists AE-58054, SB-258585, and SB-271046, had no significant effect on 5-HT neuron firing. Simultaneous electroencephalogram (EEG) recordings found that WAY-181187 and WAY-208466 reduced frontal cortex slow wave (SW) oscillation power to 62% and 60% below pre-drug values, respectively. It was considered that the frontal cortex may be involved in the effect of these agonists on 5-HT neuron firing. Subsequent experiments investigated the influence of 5-HT6 receptors over mPFC neural activity. WAY-181187 reduced mPFC SW oscillation power maximally to 54% below pre-drug values. This occurred in a putatively 5-HT6 receptor-dependent manner since the effect was blocked by SB-399885 and AE-58054. WAY-181187 also modulated the firing of mPFC pyramidal neurons, including those projecting to the DRN. Specifically, one population of neurons was excited by WAY-181187, with a maximum increase in firing of 161% above pre-drug firing, whereas another population was inhibited by WAY-181187, with a maximum decrease to 73% below pre-drug firing. In a final set of experiments neurotoxic mPFC lesion attenuated the excitatory effect of WAY-181187 on 5-HT neuron firing. Thus the effect of WAY-181187 at 2mg/kg was significantly reduced in lesioned rats compared to sham controls. Experiments in this thesis provide evidence to support a role of 5-HT6 receptor-mediated excitatory control of DRN 5-HT neurons via an mPFC-dependent mechanism. This control may act with previously reported feedback mechanisms to balance inhibitory and excitatory input to 5-HT neurons. Finally, 5-HT6 receptor-mediated feedback may be a useful target for modulating the 5-HT-system, such as in depression therapy.
46
Carmo, Fausto Moreira da Silva. "Estudo de polimorfismos do gene candidato, fator miogênico-5 (myf-5), em suínos." Universidade Federal de Viçosa, 2003. http://www.locus.ufv.br/handle/123456789/11145.
Повний текст джерелаАнотація:
Submitted by Nathália Faria da Silva (nathaliafsilva.ufv@gmail.com) on 2017-07-10T18:11:11Z
No. of bitstreams: 1
texto completo.pdf: 274581 bytes, checksum: 322a4953b05459797d7d07a51a3c9485 (MD5)Made available in DSpace on 2017-07-10T18:11:11Z (GMT). No. of bitstreams: 1 texto completo.pdf: 274581 bytes, checksum: 322a4953b05459797d7d07a51a3c9485 (MD5) Previous issue date: 2003-01-30
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Com o desenvolvimento de técnicas de biologia molecular e o uso dos marcadores de DNA, tem sido possível associar regiões genômicas com características de importância econômica. Algumas regiões genômicas possuem genes que exercem efeitos sobre determinada característica. Nessa mesma região, podem existir seqüências polimórficas, não codificadas, que servem como marcadores e, se estiverem em desequilíbrio de ligação, podem ser seguidas através de gerações de famílias formadas por linhagens divergentes. A formação dessas famílias facilita as análises de associações entre as regiões genômicas e as características fenotípicas analisadas, geralmente, na geração F2. Genes seqüenciados, com ação biológica conhecida, poderão ter formas alélicas identificadas, as quais poderão ser usadas em Seleção Assistida por Marcador, Marker Assisted Selection-MAS, aumentando a rapidez na resposta à seleção. Com o intuito de identificar novas formas alélicas, o gene candidato para o desenvolvimento muscular hiperplásico, fator miogênico-5 (mfy-5), de animais parentais, de uma geração F2 formada por machos da raça nativa Piau e fêmeas comerciais, foi amplificado por meio da reação em cadeia da polimerase - Polymerase Chain Reaction (PCR) e seqüenciado, utilizando-se seqüenciamento automático pelo método de Sanger. As seqüências geradas foram comparadas com uma seqüência homologa depositada no GenBank. Foram constatadas deleções e inserções de 1 até 3 pares de bases nas seqüências obtidas da geração parental. Essas mutações geraram diferenças alélicas observadas na F2. Os genótipos na geração F2 apresentaram duas classes mais comuns: Normal/Normal (NN) e Normal/Inserção (NI). Estes genótipos foram correlacionados, por meio de modelos estatísticos, às características de desempenho, características de carcaça e qualidade da carne. Os resultados foram significativos (P<0,05), sendo o alelo “I” responsável por maior peso de paleta, maior perda d’água por gotejamento, cozimento e perda total. O novo alelo pode ser usado em programas de seleção, nos quais deve ser monitorado e mesmo associado com outras características de qualidade da carne.
With the development of molecular biology techniques by means of DNA markers, it has been possible to associate chromosomic regions with economic traits. Some chromosomic regions have genes that produce effects on a given trait, those areas have not coded polymorphic sequences either, that serve as markers and if they are in linkage disequilibrium, they can be followed through generations of families formed by divergent lines that facilitate the analyses, because of the largest difference presented in F2 generation. It still exists the possibility of genes with great effect in economic traits that are responsible for phenotypic evaluated effects. Sequenced genes, with known biological action, may have allelic variants identified and, if they present phenotypic differences in the analyzed traits, those allelic variants can be used in Marker Assisted Selection-MAS. In the present work, a candidate gene, related to muscular hiperphasic development, was PCR amplified and sequenced by the Sanger method in parental animals of a F2 population, formed by two Brazilian Native boais (Piau breed) and 18 commercial females. The generated sequences were compared to the GenBank sequence number Y17154. Deletions and insertions of one up to three bases in the viiisequences obtained from some parental animals were observed. The genotypes in the F2 generation were lifted up and tended as more common, two classes Normal/Normal (NN) and Normal/Insertion (NI). The genotype were associated, by means of statistical models, to performance, carcass and meat quality traits. The results were significant for picnic shoulder weight, brightness, drip, cooking and total loss. The new allele may be used in breeding programs, where it must be monitored and even associated to the other meat quality traits.
47
Radeau, Thierry. "Rôle de l'acide 5-hydroxyéicosatétraénoi͏̈que (5-HETE) dans l'activation du neutrophile chez l'asthmatique." Montpellier 2, 1992. http://www.theses.fr/1992MON20123.
Повний текст джерелаАнотація:
Dans des conditions de synthese maximale, nous avons montre que la production de ltb4 par les neutrophiles d'asthmatiques en intercrise est augmentee par rapport aux neutrophiles temoins. L'augmentation du chimiotactisme induit par le paf et de la fluidite membranaire des neutrophiles des asthmatiques suggerent que ces cellules sont dans un etat pre-active. Nous avons obtenu une correlation inverse entre le chimiotactisme induit par le paf et la presence de 5-hete neo-synthetise a l'interieur de la cellule, suggerant que la generation de 5-hete pouvait etre liee a la migration des neutrophiles. D'autre part, les neutrophiles sont capables de synthetiser des quantites importantes de 5-hete. Le 5-hete est rapidement esterifie dans les membranes des cellules, en particulier dans les glycerophospholipides. Nous avons montre que le 5-hete est plus facilement incorpore dans les membranes des neutrophiles de sujets asthmatiques. Le 5-hete exogene est capable de s'esterifier dans les membranes des neutrophiles et d'amplifier le chimiotactisme induit par le paf. Nous avons trouve que le nedocromil de sodium pouvait modifier la distribution du 5-hete dans les neutrophiles des asthmatiques, suggerant ainsi un mode d'action possible pour cet anti-inflammatoire. Nos resultats suggerent que le 5-hete est un mediateur endogene d'activation cellulaire important, qui pourrait etre implique dans le mecanisme de pre-activation cellulaire
48
Lemaitre, Stéphane. "Contribution à l'étude de nouveaux ligands des récepteurs 5-HT4, 5- HT6 et 5- HT7 contenant le système pyrrolo[1,2-a]pyrazine." Caen, 2001. http://www.theses.fr/2001CAEN4001.
Повний текст джерела
49
Hardisty, Robyn Elizabeth. "Methods to probe the function of modified bases in DNA." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/271807.
Повний текст джерелаАнотація:
This thesis is focused on the development and utilisation of chemical and biological tools to probe the function of modified bases in DNA with specific exploration of the less well-studied T-modifications: 5-hmU, 5-fU and Base J. LCMS/MS techniques are first utilised to enable the accurate global quantification of T-modifications (5-hmU, 5-fU and Base J) in both trypanosomatids and mammalian DNA. A chemical affinity-enrichment sequencing method for the T-modifications is next described, which allows their chemoselective tagging over their C-modification counterparts. DNA fragments containing 5-fU are selectively tagged and enriched via oxime, hydrazine or benzimidazole formation using a biotinylated probe, and DNA fragments containing 5-hmU can be first chemically oxidised to 5-fU using KRuO4. .Proof-of-principle T-modification enrichment is demonstrated by DNA sequencing. In the following chapter, sequencing methods are employed to investigate the role of T-modifications in both trypanosomatids and mammalian samples. In T.Brucei, Base J formation is probed by artificial incorporation of 5-hmU and subsequent Base J chemical sequencing. Base J is preferentially formed or depleted at certain genomic loci; suggesting that Base J formation is sequence-specific. This may imply a distinct role for the 5-hmU sites which are not further glucosylated. Next, 5-hmU enrichment sequencing is performed in SMUG1 knockdown HEK293T cells to determine the genomic location of 5-hmU in mammals. An increase in 5-hmU loci is observed upon SMUG1 knockdown. 5-hmU enriched regions are found to be T-rich and depleted in exons and promoters. Furthermore, 5-hmU sites show poor overlap with known TET-enzyme binding sites, indicating that 5-hmU is formed via a TET-independent mechanism in HEK293T cells. Next, mass spectrometry-based proteomics studies are utilised to determine 5-fU protein-binders in mammals. Pulldown of proteins using biotinylated baits enables the identification of proteins which are enriched or suppressed in the presence of the 5-fU modification compared to a non-modified control. Enriched proteins include those associated with DNA-damage, consistent with the current understanding that 5-fU is a product of oxidative damage in mammalian DNA. Finally, a mechanistic insight into the effect of formylated bases on nucleosomal structure is described. Schiff base formation between formylated nucleobases and histone protein lysine side-chains is demonstrated. This provides a molecular mechanism for the association of 5-fC with increased nucleosomal occupancy in vivo.
50
Talbi, Hassan. "Synthèse et caractérisation électrochimique et physico-chimique du polyindole et de ses dérivés substitués : (poly-5-cyaboindole, poly-5-carboxyindole et poly-5-nitroindole)." Nancy 1, 1998. http://www.theses.fr/1998NAN10212.
Повний текст джерелаАнотація:
Ce travail qui s'inscrit dans le cadre de recherches sur les polymères conducteurs électroniques, a été consacré à la synthèse et la caractérisation du polyindole et de ses dérivés substitués en position 5 par des groupements accepteurs d'électron (-CN, -COOH, -N02). Le comportement de ces polymères a été examiné en milieu aprotique neutre et acidifié ainsi qu'en milieu aqueux acide. Les dérivés substitués, contrairement au parent polyindole, présentent des propriétés très intéressantes en milieu aqueux acide. Ces polymères présentent deux processus redox distincts et bien définis. La nette réversibilité de ces couples redox conduit naturellement à des dispositifs de stockage de l'énergie et d'affichages plus efficaces. Le couple redox de ces dérivés, situé vers les potentiels les plus positifs, est associé à un échange de protons. De plus, la variation du potentiel redox de ces polymères, en fonction du pH, ouvre une perspective intéressante pour la réalisation d'un capteur moléculaire. Nous avons utilisé différentes techniques électrochimiques (chronoampérométrie, chronopotentiométrie, voltammétrie cyclique,. . . ) pour la synthèse et l'étude du comportement électrochimique de ces polymères. L'analyse et la comparaison des phénomènes de nature électrique dans les films de polyindoles a été effectuée in-situ en utilisant la spectroscopie d'impédance complexe (BIS). Les phénomènes de conduction électrique ont été examinés en fonction de divers paramètres électrochimiques ainsi qu'en fonction de la température. Les phénomènes de vieillissement électrochimique et thermique ont été également étudiés. La modélisation du mécanisme d'oxydo-réduction du polyindole et de ses dérivés substitués, a été examinée par les spectroscopies "ex-situ" : FTIR, Raman, XPS et EELS. Nous avons également effectué une étude spectroélectrochimique "in-situ" sur ces différents polymères. Les modifications au cours du dopage électrochimique ont été suivies en spectroscopie Raman, à différentes longueurs d'ondes d'excitation du visible au proche infrarouge. Les résultats obtenus sont pour la plupart originaux. Ainsi, les analyses de comportement de ces films polymères par FTIR, Raman, XPS, EELS et EIS, permettent une compréhension plus fine des processus chimiques et électrochimiques survenant à la surface de l'électrode au cours des échanges avec une solution à base d'un solvant organique ou aqueux. La détermination de la structure de la chaîne a été examinée théoriquement en utilisant des méthodes semi-empiriques (Hartree-Fock). La description de la réaction de polymérisation à l'aide des approches par contrôles thermodynamique et cinétique semble être un outil adéquat tant pour expliquer la régiospécificité de la réaction de couplage que pour déterminer la structure de la chaîne polymère.