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Статті в журналах з теми "307.1/16"
1
Guerin, François, Mohamed Sassi, Loren Dejoies, Asma Zouari, Sacha Schutz, Sophie Potrel, Michel Auzou, et al. "Molecular and functional analysis of the novel cfr(D) linezolid resistance gene identified in Enterococcus faecium." Journal of Antimicrobial Chemotherapy 75, no. 7 (April 11, 2020): 1699–703. http://dx.doi.org/10.1093/jac/dkaa125.
Повний текст джерелаАнотація:
Abstract Objectives To characterize the novel cfr(D) gene identified in an Enterococcus faecium clinical isolate (15-307.1) collected from France. Methods The genome of 15-307.1 was entirely sequenced using a hybrid approach combining short-read (MiSeq, Illumina) and long-read (GridION, Oxford Nanopore Technologies) technologies in order to analyse in detail the genetic support and environment of cfr(D). Transfer of linezolid resistance from 15-307.1 to E. faecium BM4107 was attempted by filter-mating experiments. The recombinant plasmid pAT29Ωcfr(D), containing cfr(D) and its own promoter, was transferred to E. faecium HM1070, Enterococcus faecalis JH2-2 and Escherichia coli AG100A. Results As previously reported, 15-307.1 belonged to ST17 and was phenotypically resistant to linezolid (MIC, 16 mg/L), vancomycin and teicoplanin. A hybrid sequencing approach confirmed the presence of several resistance genes including vanA, optrA and cfr(D). Located on a 103 kb plasmid, cfr(D) encoded a 357 amino acid protein, which shared 64%, 64%, 48% and 51% amino acid identity with Cfr, Cfr(B), Cfr(C) and Cfr(E), respectively. Both optrA and cfr(D) were successfully co-transferred to E. faecium BM4107. When expressed in E. faecium HM1070 and E. faecalis JH2-2, pAT29Ωcfr(D) did not confer any resistance, whereas it was responsible for an expected PhLOPSA resistance phenotype in E. coli AG100A. Analysis of the genetic environment of cfr(D) showed multiple IS1216 elements, putatively involved in its mobilization. Conclusions Cfr(D) is a novel member of the family of 23S rRNA methyltransferases. While only conferring a PhLOPSA resistance phenotype when expressed in E. coli, enterococci could constitute an unknown reservoir of cfr(D).
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Peart Akindele, Nadine, Theodore Kouo, Andrew H. Karaba, Oren Gordon, Katherine Z. J. Fenstermacher, Jeanette Beaudry, Jessica H. Rubens, et al. "Distinct Cytokine and Chemokine Dysregulation in Hospitalized Children With Acute Coronavirus Disease 2019 and Multisystem Inflammatory Syndrome With Similar Levels of Nasopharyngeal Severe Acute Respiratory Syndrome Coronavirus 2 Shedding." Journal of Infectious Diseases 224, no. 4 (May 24, 2021): 606–15. http://dx.doi.org/10.1093/infdis/jiab285.
Повний текст джерелаАнотація:
Abstract Background Multisystem inflammatory syndrome in children (MIS-C) is a severe clinical phenotype of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection that remains poorly understood. Methods Hospitalized children <18 years of age with suspected coronavirus disease 2019 (COVID-19) (N = 53) were recruited into a prospective cohort study; 32 had confirmed COVID-19, with 16 meeting the US Centers for Disease Control criteria for MIS-C. Differences in nasopharyngeal viral ribonucleic acid (RNA) levels, SARS-CoV-2 seropositivity, and cytokine/chemokine profiles were examined, including after adjustments for age and sex. Results The median ages for those with and without MIS-C were 8.7 years (interquartile range [IQR], 5.5–13.9) and 2.2 years (IQR, 1.1–10.5), respectively (P = .18), and nasopharyngeal levels of SARS-CoV-2 RNA did not differ significantly between the 2 groups (median 63 848.25 copies/mL versus 307.1 copies/mL, P = .66); 75% of those with MIS-C were antibody positive compared with 44% without (P = .026). Levels of 14 of 37 cytokines/chemokines (interleukin [IL]-1RA, IL-2RA, IL-6, IL-8, tumor necrosis factor-α, IL-10, IL-15, IL-18, monocyte chemoattractant protein [MCP]-1, IP-10, macrophage-inflammatory protein [MIP]-1α, MCP-2, MIP-1β, eotaxin) were significantly higher in children with MIS-C compared to those without, irrespective of age or sex (false discovery rate <0.05; P < .05). Conclusions The distinct pattern of heightened cytokine/chemokine dysregulation observed with MIS-C, compared with acute COVID-19, occurs across the pediatric age spectrum and with similar levels of nasopharyngeal SARS-CoV-2 RNA.
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Raza, Syed Turab, Sana Hafeez, Zulfiqar Ali, Zaheer Ahmad Nasir, Muhammad Moeen Butt, Irfan Saleem, Jianping Wu, Zhe Chen, and Yunjian Xu. "An Assessment of Air Quality within Facilities of Municipal Solid Waste Management (MSWM) Sites in Lahore, Pakistan." Processes 9, no. 9 (September 7, 2021): 1604. http://dx.doi.org/10.3390/pr9091604.
Повний текст джерелаАнотація:
The pollutants emission during the process of municipal solid waste management (MSWM) is of great concern due to its hazardous effect on the environment and living organisms. An assessment of the air quality of MSWM sites was made after having 16 repetitive visits at solid waste disposal sites and transfer stations of Lahore during wet and dry seasons. Pollution parameters such as fine particulate matter (PM2.5) and greenhouse gases (GHG) were measured along with meteorological parameters. PM2.5 measurement was made by using particle counter Dylos and TSI’s Dust Trak. Both of these instruments were positioned simultaneously at the source site and downwind (50 m). CH4 and meteorological parameters were measured by Aeroqual 500 series, while the Extech CO220 monitor was used to measure CO2 concentration. An assessment of air quality showed the levels of their mean values as CH4 and CO2 ranged between 1.5–13.7 ppm and 443.4–515.7 ppm, respectively. The PM2.5 ranged between 127.1 and 307.1 µg/m3 at sources and 172.3 and 403.8 µg/m3 downwind (50 m). GHG showed lower levels than the proposed limit value, which could not cause any health issues, while PM2.5 was 6–10 times higher than the Pak-EPA established standards. Higher pollutant concentration was recorded in the dry season than the wet season. Regression analysis was performed to predict correlation of PM2.5 with GHG and meteorological parameters. GHG as well as meteorological parameters also exhibited a correlation with PM2.5. It was estimated that the ambient air of such sites is not safe for public health. So, it is necessary to use safe practices for MSWM and its emission control to prevent nearby communities and the environment.
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Eloy, Jean Anderson, Thomas J. Walker, Roy R. Casiano, and Jose W. Ruiz. "Effect of Coblation Polypectomy on Estimated Blood Loss in Endoscopic Sinus Surgery." American Journal of Rhinology & Allergy 23, no. 5 (September 2009): 535–39. http://dx.doi.org/10.2500/ajra.2009.23.3330.
Повний текст джерелаАнотація:
Background We conducted a pilot study comparing estimated blood loss (EBL) using coblation-assisted endoscopic sinus surgery (CAESS) where coblation is used to debulk nasal polyps before microdebridement with a traditional microdebrider technique in chronic rhinosinusitis (CRS) patients with sinonasal polyps undergoing endoscopic sinus surgery (ESS). Methods A retrospective analysis was performed at a tertiary care center on patients with nasal polyposis undergoing ESS between January 2008 and July 2008. The University of Miami CT staging system was used preoperatively to evaluate the extent of sinonasal disease. The duration of surgery, blood loss per minute, total EBL, and demographic data were collected. Results Twenty-one patients underwent nasal polypectomy/ESS using CAESS and 16 patients underwent nasal polypectomy/ESS using microdebridement. The two groups had comparable University of Miami CT staging scores (p > 0.05). The average EBL was 307.1 ± 169.8 mL using coblation compared with 627.8 ± 424.2 mL using microdebridement (p < 0.05). The average duration of surgery using coblation was 116.2 ± 41.7 minutes, compared with 125.3 ± 48.4 minutes using microdebridement (p > 0.05). The average blood loss per minute was 2.8 ±1.7 mL in the coblation group compared with 4.8 ± 2.1 mL in the microdebridement group (p < 0.05). Subgroup analyses showed a significant decrease in average EBL and EBL/minute to be only significant for revision cases (p < 0.05) and not for primary cases (p > 0.05). Conclusion Coblation-assisted nasal polypectomy/ESS is associated with a statistically significant lower EBL and blood loss per minute when compared with traditional microdebridement technique. Coblation represents a new device that can reduce blood loss in patients with nasal polyposis undergoing traditional revision ESS. Further prospective randomized trials are needed to validate these findings.
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Mangine, Gerald T., Jay R. Hoffman, Jose Vazquez, Napoleon Pichardo, Maren S. Fragala, and Jeffrey R. Stout. "Predictors of Fielding Performance in Professional Baseball Players." International Journal of Sports Physiology and Performance 8, no. 5 (September 2013): 510–16. http://dx.doi.org/10.1123/ijspp.8.5.510.
Повний текст джерелаАнотація:
The ultimate zone-rating extrapolation (UZR/150) rates fielding performance by runs saved or cost within a zone of responsibility in comparison with the league average (150 games) for a position. Spring-training anthropometric and performance measures have been previously related to hitting performance; however, their relationships with fielding performance measures are unknown.Purpose:To examine the relationship between anthropometric and performance measurements on fielding performance in professional baseball players.Methods:Body mass, lean body mass (LBM), grip strength, 10-yd sprint, proagility, and vertical-jump mean (VJMP) and peak power (VJPP) were collected during spring training over the course of 5 seasons (2007–2011) for professional corner infielders (CI; n = 17, fielding opportunities = 420.7 ± 307.1), middle infielders (MI; n = 14, fielding opportunities = 497.3 ± 259.1), and outfielders (OF; n = 16, fielding opportunities = 227.9 ± 70.9). The relationships between these data and regular-season (100-opportunity minimum) fielding statistics were examined using Pearson correlation coefficients, while stepwise regression identified the single best predictor of UZR/150.Results:Significant correlations (P < .05) were observed between UZR/150 and body mass (r = .364), LBM (r = .396), VJPP (r = .397), and VJMP (r = .405). Of these variables, stepwise regression indicated VJMP (R = .405, SEE = 14.441, P = .005) as the single best predictor for all players, although the addition of proagility performance strengthened (R = .496, SEE = 13.865, P = .002) predictive ability by 8.3%. The best predictor for UZR/150 was body mass for CI (R = .519, SEE = 15.364, P = .033) and MI (R = .672, SEE = 12.331, P = .009), while proagility time was the best predictor for OF (R = .514, SEE = 8.850, P = .042).Conclusions:Spring-training measurements of VJMP and proagility time may predict the defensive run value of a player over the course of a professional baseball season.
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Travnicek, J., V. Kroupova, I. Herzig, and J. Kursa. "Iodine content in consumer hen eggs." Veterinární Medicína 51, No. 3 (March 19, 2012): 93–100. http://dx.doi.org/10.17221/5526-vetmed.
Повний текст джерелаАнотація:
This paper presents the latest information about the supply of iodine to meet its requirements in hens of laying type, on the basis of iodine content in egg yolk and about the importance of eggs as an iodine source in human nutrition. The Sandell-Kolthoff method was applied to determine iodine content in the yolk of eggs from 9 large flocks (54 eggs) and 16 small flocks (96 eggs) in 2004, and from 10 large flocks (135 eggs) and 15 small flocks (114 eggs) in 2005. Iodine content was also determined in the albumen of 70 eggs. In 2004, the iodine content in the yolk of eggs from large flocks was 1 014.1 ± 356.6 while in 2005 it amounted to 1 663.8 ± 1 179.7 µg/kg fresh matter (P < 0.01); the respective values for the yolk of eggs from small flocks in 2004 and 2005 were 307.1 ± 255.7 and 519.5 ± 508.2 µg/kg fresh matter (P < 0.01). Compared to 1996, in 2005, the iodine content in yolk increased by 123.7% in large flocks, and by 19.2% in small flocks. The iodine content in albumen was 16.2 ± 9.7 µg/kg fresh matter, and it accounted for 2.6–5.0% of the total iodine content in 1 egg. The correlation coefficient between iodine contents in yolk and albumen was r = 0.67. In the Czech Republic, 1 egg from large flocks contains on average 31.2 µg iodine while 1 egg from small flocks contains 10.0 µg. Eggs from large flocks cover 7–14% and from small flocks 2.2–4.4% of the daily iodine requirement in adults. Iodine concentrations exceeding 2 500 µg/kg yolk fresh matter in large flocks were measured between week 32 and 60 of the laying cycle and with daily intake of 0.116–0.132 mg iodine per hen.
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Marchenko, L. A., I. G. Smirnov, R. K. Kurbanov, and A. Yu Spiridonov. "Operational Costs of Unmanned Aircraft System for Applying Pesticides and Fertilizers." Agricultural Machinery and Technologies 16, no. 1 (March 18, 2022): 53–62. http://dx.doi.org/10.22314/2073-7599-2022-16-1-53-62.
Повний текст джерелаАнотація:
The calculation of aircraft cost is stated necessary to assess the economic feasibility of their operation, as well as in agriculture, the possibility of acquiring new ones, and to justify air transportation tariffs. (Research purpose) The research purpose is to substantiate the methodology and calculate the prime cost of using BAS-137 VIM unmanned aerial system for the application of pesticides, fertilizers and other agrochemicals. (Materials and methods) When developing the methodology for calculating the prime cost of using an unmanned aerial system for applying pesticides and fertilizers, the Guidelines for determining the cost of domestic and international flights for Russian airlines were used. (Results and discussion) We have obtained the main dependences of the prime cost of using BAS-137 VIM for the introduction of working fluids: on the application rate, the field rut length, the distance of approach to the refueling place, and the operating flight speed. (Conclusions) The authors developed a methodology for calculating the prime cost of using an unmanned aerial system for applying pesticides and fertilizers, including direct and indirect operating costs. It was found out that at the working fluid application rates of 5-100 liters per hectare, the changes in the cost of using BAS-137 VIM are within the range from 280.9 to 1315.5 rubles per hectare. At an application rate of 10 liters per hectare within the rut length of 0.3-2.0 kilometers, the application prime cost is 307.1-529.5 rubles per hectare. It was obtained that at the application rates of 5-20 liters per hectare and the rut length of 0.6-1.0 kilometers, the prime cost will be the lowest, within the range of 264-369 rubles per hectare at an operating flight speed of 60 kilometers per hour and within the range of 482-587 rubles per hectare at a flying speed of 30 kilometers per hour. It was shown that with a twofold increase in BAS-137 VIM operating speed, the prime cost decreases by 1.2-1.5 times.
Книги з теми "307.1/16"
1
Soft City: Building Density for Everyday Life. Island Press, 2019.
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2
Soft City: Building Density for Everyday Life. Island Press, 2019.
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