Готові списки джерел за темами / 3-dimensional cell culture

Добірка наукової літератури з теми "3-dimensional cell culture"

Автор: Grafiati
Опубліковано: 13 вересня 2022

Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями

Оберіть тип джерела:

Зміст

Ознайомтеся зі списками актуальних статей, книг, дисертацій, тез та інших наукових джерел на тему "3-dimensional cell culture".

Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.

Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.

Статті в журналах з теми "3-dimensional cell culture"

1

KODAMA, Makoto. "New System of 3 Dimensional Cell Culture." Journal of the Society of Mechanical Engineers 119, no. 1169 (2016): 218–21. http://dx.doi.org/10.1299/jsmemag.119.1169_218.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Ravi, Maddaly, Aishwarya Pargaonkar, Anuradha Ramesh, Gatika Agrawal, Jennifer Sally, SriVijayaGanapathy Srinivasan, and Abhishek Kalra. "Three-dimensional prints from 3-dimensional cell culture aggregates of human cancer cell lines." Sri Ramachandra Journal of Health Sciences 1 (December 24, 2021): 10–15. http://dx.doi.org/10.25259/srjhs_5_2021.

Повний текст джерела
Анотація:
Objectives: Three-dimensional (3D) printing has gained significance for human health-care applications in recent years. Some of these applications include obtaining models which mimic anatomical parts. One other parallel development in the biological research area is the development of 3D cell cultures. Such cultures are now becoming the material of choice for in vitro experiments, fast replacing the traditional adherent/monolayer 2D culture approaches. We present here, a method to obtain 3D prints of 3D aggregates of three human cancer cell lines. Such 3D prints can be useful models to understand solid tumor morphologies and also as effective teaching models. Materials and Methods: Photomicrographs of the 3D aggregates of the human cancer cell lines SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) were obtained using inverted phase contrast microscopy. Conversion of normal jpeg images into 3D files was performed using the lithophane method and CAD files obtained. The CAD files thus generated were used to print the objects using the Stratasys Polyjet J750 3D Printer. Results: We could obtain 3D prints of SiHa, MCF-7, and A549 (human cervical cancer, breast cancer, and non-small cell lung cancer cell lines, respectively) 3D aggregates/spheroids. Conclusion: It is hoped that this approach will be useful for studying solid tumor morphologies in finer details. Furthermore, other benefits of such 3D prints would be in them being excellent models for teaching purposes.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Kupchik, H. Z., E. A. Collins, M. J. O'Brien, and R. P. McCaffrey. "Chemotherapy screening assay using 3-dimensional cell culture." Cancer Letters 51, no. 1 (May 1990): 11–16. http://dx.doi.org/10.1016/0304-3835(90)90224-l.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Lee, J., M. Park, J. Byeon, N. Gu, I. Cho, and S. Cha. "Angiogenic effects of 3 dimensional cell culture system." Cytotherapy 19, no. 5 (May 2017): S234—S235. http://dx.doi.org/10.1016/j.jcyt.2017.02.344.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Chitturi Suryaprakash, Ravi Teja, Omar Kujan, Kate Shearston, and Camile S. Farah. "Three-Dimensional Cell Culture Models to Investigate Oral Carcinogenesis: A Scoping Review." International Journal of Molecular Sciences 21, no. 24 (December 14, 2020): 9520. http://dx.doi.org/10.3390/ijms21249520.

Повний текст джерела
Анотація:
Three-dimensional (3-D) cell culture models, such as spheroids, organoids, and organotypic cultures, are more physiologically representative of the human tumor microenvironment (TME) than traditional two-dimensional (2-D) cell culture models. They have been used as in vitro models to investigate various aspects of oral cancer but, to date, have not be widely used in investigations of the process of oral carcinogenesis. The aim of this scoping review was to evaluate the use of 3-D cell cultures in oral squamous cell carcinoma (OSCC) research, with a particular emphasis on oral carcinogenesis studies. Databases (PubMed, Scopus, and Web of Science) were systematically searched to identify research applying 3-D cell culture techniques to cells from normal, dysplastic, and malignant oral mucosae. A total of 119 studies were included for qualitative analysis including 53 studies utilizing spheroids, 62 utilizing organotypic cultures, and 4 using organoids. We found that 3-D oral carcinogenesis studies had been limited to just two organotypic culture models and that to date, spheroids and organoids had not been utilized for this purpose. Spheroid culture was most frequently used as a tumorosphere forming assay and the organoids cultured from human OSCCs most often used in drug sensitivity testing. These results indicate that there are significant opportunities to utilize 3-D cell culture to explore the development of oral cancer, particularly as the physiological relevance of these models continues to improve.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Ceresa, Claudia C., Alan J. Knox, and Simon R. Johnson. "Use of a three-dimensional cell culture model to study airway smooth muscle-mast cell interactions in airway remodeling." American Journal of Physiology-Lung Cellular and Molecular Physiology 296, no. 6 (June 2009): L1059—L1066. http://dx.doi.org/10.1152/ajplung.90445.2008.

Повний текст джерела
Анотація:
Increased airway smooth muscle (ASM) mass and infiltration by mast cells are key features of airway remodeling in asthma. We describe a model to investigate the relationship between ASM, the extracellular matrix, mast cells, and airway remodeling. ASM cells were cultured in a three-dimensional (3-D) collagen I gel (3-D culture) alone or with mast cells. Immunocytochemistry and Western blotting of ASM in 3-D cultures revealed a spindle-shaped morphology and significantly lower α-smooth muscle actin and vimentin expression than in ASM cultured in monolayers on collagen type I or plastic (2-D culture). In 3-D cultures, basal ASM proliferation, examined by Ki67 immunocytochemistry, was reduced to 33 ± 7% ( P < 0.05) of that in 2-D cultures. The presence of mast cells in cocultures increased ASM proliferation by 1.8-fold ( P < 0.05). Gelatin zymography revealed more active matrix metalloproteinase (MMP)-2 in 3-D than in 2-D culture supernatants over 7 days. Functional MMP activity was examined by gel contraction. The spontaneous gel contraction over 7 days was significantly inhibited by the MMP inhibitor ilomastat. Mast cell coculture enhanced ASM gel contraction by 22 ± 16% (not significant). Our model shows that ASM has different morphology, with lower contractile protein expression and basal proliferation in 3-D culture. Compared with standard techniques, ASM synthetic function, as shown by MMP production and activity, is sustained over longer periods. The presence of mast cells in the 3-D model enhanced ASM proliferation and MMP production. Airway remodeling in asthma may be more accurately modeled by our system than by standard culture systems.
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Kim, Minseok S., Ju Hun Yeon, and Je-Kyun Park. "A microfluidic platform for 3-dimensional cell culture and cell-based assays." Biomedical Microdevices 9, no. 1 (November 11, 2006): 25–34. http://dx.doi.org/10.1007/s10544-006-9016-4.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Jenkins, James, Ruslan I. Dmitriev, Karl Morten, Kieran W. McDermott, and Dmitri B. Papkovsky. "Oxygen-sensing scaffolds for 3-dimensional cell and tissue culture." Acta Biomaterialia 16 (April 2015): 126–35. http://dx.doi.org/10.1016/j.actbio.2015.01.032.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Wu, Min-Hsien, Yu-Han Chang, Yen-Ting Liu, Yan-Ming Chen, Shih-Siou Wang, Hsin-Yao Wang, Chao-Sung Lai, and Tung-Ming Pan. "Development of high throughput microfluidic cell culture chip for perfusion 3-dimensional cell culture-based chemosensitivity assay." Sensors and Actuators B: Chemical 155, no. 1 (July 2011): 397–407. http://dx.doi.org/10.1016/j.snb.2010.11.027.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Ferraz, M. A. M. M., H. H. W. Henning, K. M. A. Van Dorenmalen, P. L. A. M. Vos, T. A. E. Stout, P. F. Costa, J. Malda, and B. M. Gadella. "52 USE OF TRANSWELL CELL CULTURE AND 3-DIMENSIONAL PRINTING TECHNOLOGY TO DEVELOP AN IN VITRO BOVINE OVIDUCT." Reproduction, Fertility and Development 28, no. 2 (2016): 156. http://dx.doi.org/10.1071/rdv28n2ab52.

Повний текст джерела
Анотація:
Oviduct epithelial cells (OECs) generate the microenvironment for mammalian fertilization. When cultured in vitro OECs rapidly lose their differentiated cell properties (e.g. secretory activity and cilia), while suspended cells have a limited lifespan. These limitations, likely due to the lack of folded tubular geometry of the oviduct, prompted us to combine transwell cell culture and 3-D printing technologies to mimic the in vivo OEC niche in order to better study the unique role of the oviduct and its microenvironment during the processes of fertilization and early embryonic development. U-shape inserts were 3-D printed using a multi-arm acrylate-based resin (PIC100) on an Envisiontec Perfactory P3 stereolithographer. Post-printing treatments of custom-made tubular transwell inserts were first tested in order to determine any possible negative impact of the plastics on cell growth. Inserts were either untreated, post-cured with 1000 flashes/side (Otoflash, 66 W), post-cured and Soxhlet-extracted overnight in isopropanol, or post-cured and Soxhlet-extracted over the weekend in water at 37°C. The post-cured and Soxhlet-extracted overnight in isopropanol inserts were selected as best pretreatment for culturing OECs. These inserts were mounted with track-etched PET membranes (12 µm thick, 0.4 µm pore diameter) to create a U-shape geometry that allows perfusion. Bovine OECs were obtained by squeezing the whole oviduct collected from slaughterhouse cows (on luteal phase) and cultured as monolayers for 7 days (n = 2 cows). These de-differentiated OECs were seeded on the membranes, grown to confluence (7 days), and cultured (1) at an air-liquid interface for 6 and 14 days (air-liquid culture) or (2) under perfusion (6 mL h–1) for 6 days (perfusion culture). OECs were also cultured on coverslips as monolayers (2-D culture) for 6 and 14 days. After this period, the OECs were fixed and immune labelled to determine their polarized state. Polarization of OECs (laminin and primary cilia detection) was observed on Day 6 for perfusion culture, on Day 14 for air-liquid culture, and was not detected in 2-D culture. The presence of secondary cilia (acetylated α-tubulin) was observed in 6% of the cells cultured under perfusion at Day 6; secondary cilia was not present in air-liquid or 2-D cultures during the period analysed. In conclusion, post-curing and Soxhlet extraction of leachable compounds is crucial to avoid toxic effects on cell growth. The U-shape custom-designed inserts are able to create a tube-like surface in which bovine oviducal cells can be cultured to confluency and thereafter repolarize (presence of primary cilia and detection of laminin); this polarization occurs faster when the U-shape culture is under perfusion. Further studies will examine the ability of the cells to differentiate further (development of secondary cilia and secretory ability) and support in vitro fertilization. To this end, 3-D designs will be tested to determine their use for live cell imaging and for collecting secreted fluids.
Стилі APA, Harvard, Vancouver, ISO та ін.
Більше джерел

Дисертації з теми "3-dimensional cell culture"

1

Vishnolia, Krishan Kumar. "Development and charaterisation of 3 dimensional culture models for zebrafish (Danio rerio) skeletal muscle cells." Thesis, University of Bedfordshire, 2013. http://hdl.handle.net/10547/556396.

Повний текст джерела
Анотація:
Zebrafish (Danio rerio) have been extensively used over the past two decades to study muscle development, human myopathies and dystrophies, due to its higher degree of homology with human disease causing genes and genome. Despite its unique qualities, zebrafish have only been used as an in-vivo model for muscle development research, due to the limitations surrounding lack of a consistent isolation and culture protocol for zebrafish muscle progenitor cells in-vitro. Using different mammalian myoblast isolation protocols, a novel and robust protocol has been developed to successfully isolate and culture zebrafish skeletal muscle cells repeatedly and obtain differentiated long multi nucleated zebrafish myotubes. Commitment to myogenic lineage was confirmed by immuno-staining against muscle specific protein desmin, and expression pattern of different genetic markers regulating myogenesis. In order to recapitulate the in-vivo bio-physiological environment for zebrafish skeletal muscle cells in-vitro, these cells were successfully cultured in tissue engineered three dimensional (3D) constructs based on fibrin and collagen models. Maturation of tissue engineered collagen and fibrin based constructs was confirmed using the basic parameters described in the literature i.e. collagen three times greater contraction from the original width (Mudera, Smith et al. 2010) and fibrin constructs tightly coiled up to 4mm of diameter (Khodabukus, Paxton et al. 2007). In-vitro characterisation of zebrafish skeletal muscle cells showed hypertrophic growth of muscle mass compared to hyperplasic growth in-vivo as suggested for fish species in literature (Johnston 2006), which is different from human and other mammals. Comparative analysis of zebrafish muscle cells cultured in monolayer against cultured in 3D tissue engineered constructs showed significant increase in fusion index, nuclei per myotube (two-fold) and myotubes per microscopic frame (two-fold). Cells cultured in tissue engineered construct closely resembled in-vivo muscle in terms of their unidirectional orientation of myotubes. These tissue engineered 3D zebrafish skeletal muscle models could be used for various purposes such as drug screening, effect of different temperature extremes, studying underlined pathways involved in human diseases; and with further refinements it would potentially replace the need for studies on live fish in these areas.
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Chan, Yannie Ka Yan. "Evaporation-induced 3-dimensional diblock copolymer micelles micropattern : applications as templated polymeric microwells for cell culture scaffold, bioanalytic arrays and micro-silver networks /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BIEN%202004%20CHAN.

Повний текст джерела
Анотація:
Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2004.
Includes bibliographical references (leaves 122-133). Also available in electronic version. Access restricted to campus users.
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Häger, Jan-Dirk [Verfasser]. "Establishment of a bovine placental trophoblast cell line and a 3-dimensional spheroid culture model: biological effects of epidermal growth factor (EGF) / Jan-Dirk Häger." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2011. http://d-nb.info/1013334027/34.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Hoque, Apu E. (Ehsanul). "Migration and invasion pattern analysis of oral cancer cells in vitro." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526220239.

Повний текст джерела
Анотація:
Abstract Desmoglein 3 (Dsg3) is an adhesion receptor in desmosomes, but relatively little is known about its role in cancer. In this study, the function of Dsg3 was investigated in oral squamous cell carcinoma (SCC) cell lines in vitro using locally established human leiomyoma tumor microenvironment (TME) matrices. Since Dsg3 has been identified as a key regulator in cell adhesion, we hypothesized that it may play a role in oral SCC cells adhesion and motility. Thus, one aim of the study was to explore this hypothesis by both gain and loss of function methods in four human buccal mucosa SCC SqCC/Y1 cell lines: transduction of vector control (Ct), full-length (FL) or two different C-terminally truncated Dsg3 mutants (Δ238 and Δ560). Live cell imaging was performed for 2D migration and 3D sandwich, alongside other assays. In 3D sandwich, we tested the effects of the monoclonal antibody, AK23, targeting the extracellular domain of Dsg3 in SqCC/Y1 cells. Our results showed that loss of Dsg3 disrupted cell adhesion and protein expression. In 2D assays, FL and Dsg3 mutants migrated faster with higher accumulated distances than Ct. In contrast with 2D, mutants showed accelerated invasion over the Ct in 3D models. The AK23 antibody inhibited only the invasion of FL cells. The TME in vivo consists of cellular and matrix elements playing a leading role in carcinoma progression. To study carcinoma cells invasion in vitro, mouse Matrigel® and rat type 1 collagen are the most commonly used matrices in 3D models. Since they are non-human in origin, they do not perfectly mimic human TME. To address this, we have developed a solid organotypic myoma disc model derived from human uterus leiomyoma tumor. Here, we introduce a novel Myogel, prepared from leiomyoma similar to Matrigel®. We validated Myogel for cell-TME interactions in 3D models, using SqCC/Y1 and HSC-3 cell lines. Compared with Matrigel® and type I collagen, oral SCC cell lines invaded more efficiently in Myogel containing matrices. This study describes promising 3D models using human TME mimicking Myogel which is suitable to analyze oral SCC cells both in carcinoma monocultures and in co-cultures, such as with TME fibroblasts. We also introduce a possible novel therapeutic target against Dsg3 to suppress cancer cell invasion
Tiivistelmä Desmogleiini 3 (Dsg3) on desmosomien adheesioreseptori, jonka merkityksestä syövässä tiedetään vähän. Koska Dsg3 on tärkeä epiteelisolujen välisissä liitoksissa, oletimme sillä olevan vaikutusta myös suun karsinoomasolujen tarttumisessa ja niiden liikkuvuudessa. Testasimme hypoteesiamme muuttamalla Dsg3:n toimintaa ihmisen posken karsinoomasolulinjassa SqCC/Y1, josta oli aiemmin valmistettu neljä erilaista muunnosta: tyhjän vektorin sisältävä kontrollisolulinja (Ct), kokopitkää Dsg3 tuottava solulinja (FL), sekä kaksi Dsg3 C-päästä lyhennettyä mutanttisolulinjaa (Δ238 ja Δ560). Immunofluoresenssi-menetelmää käyttäen analysoimme solulinjoissamme solujen välisiä liitoksia. Lisäksi mittasimme solujen liikkeitä 2D-migraatio- ja 3D-sandwich-kokeissa. Testasimme myös Dsg3:n solunulkoista osaa tunnistavan monoklonaalisen vasta-aineen (AK23) vaikutusta solujen invaasioon. Osoitimme, että Dsg3:n rakenteen muuttaminen ja toiminnan estyminen häiritsi solujen tarttumista. 2D-kokeissa sekä FL että mutanttilinjat (Δ238 ja Δ560) migroivat kontrollisoluja nopeammin ja pidemmälle, mutta 3D-kokeissa vain mutanttilinjat invasoituivat kontrollisoluja tehokkaammin. AK23-vasta-aine esti vain FL-solujen invaasiota. Syöpäsolujen 3D-invaasiota mittaavissa kokeissa käytetään yleensä hiiren kasvaimesta valmistettua kaupallista Matrigeeliä® tai rotan kudoksista eristettyä tyypin I kollageenia. Tutkimusryhmämme on jo aiemmin kehittänyt organotyyppisen myoomamallin, jossa valmistamme myoomakudosnapit ihmisen kohdun leiomyoomakasvaimista. Tässä työssä valmistimme leiomyoomasta Myogeelia, vertasimme sitä Matrigeeliin®, sekä tutkimme tarkemmin Myogeeli-valmisteen soveltuvuutta 3D-tutkimuksiin. Totesimme, että kielen (HSC-3) ja posken (SqCC/Y1) karsinoomasolut invasoituivat tehokkaimmin Myogeeli-pitoisissa matrikseissa kuin Matrigeeliä® tai kollageeniä sisältävissä kasvatusalustoissa. Tutkimustulostemme perusteella Myogeeli-pohjaiset 3D-mallit soveltuvat hyvin sekä syöpäsolulinjojen invaasiotutkimuksiin että yhteisviljelmiin, joissa syöpäsoluja viljellään yhdessä syöpäkasvaimen ympärillä olevien solujen, kuten fibroblastien, kanssa
Стилі APA, Harvard, Vancouver, ISO та ін.
5

De, Conto Véronique. "Importance du microenvironnement dans les modèles cérébraux in vitro pour le criblage phénotypique." Thesis, Université de Lille (2018-2021), 2021. http://www.theses.fr/2021LILUS046.

Повний текст джерела
Анотація:
Environ 90% des candidats-médicaments échouent en phase clinique, pour des raisons d’efficacité ou de toxicité qui impliquent souvent le système nerveux central (SNC). Ce fort taux d’échec souligne un manque de pertinence des modèles expérimentaux utilisés en amont, dont les modèles in vitro de cellules humaines. En effet, ces derniers ne prennent pas en compte toute la complexité du SNC, où les neurones organisés en 3 dimensions (3D) interagissent avec leur microenvironnement composé de cellules, de facteurs solubles et des molécules de la matrice extracellulaire (MEC). Les objectifs de ce travail étaient i) d’étudier l’influence de ces trois composantes du microenvironnement sur les cellules neuronales dans des modèles cérébraux in vitro par imagerie cellulaire automatisée, et ii) de développer des modèles cérébraux in vitro plus pertinents pour évaluer les effets neurotoxiques ou thérapeutiques de molécules par criblage phénotypique, notamment dans le cadre de la maladie de Parkinson (MP).Dans un premier temps, la technologie BIOMIMESYS® Brain a été développée. Cette matrice à base d’acide hyaluronique permet de mimer la MEC et de cultiver des cellules cérébrales en 3D dans des plaques 96 puits. La sensibilité des cellules Luhmes, une lignée de neurones dopaminergiques, aux inducteurs de la MP a été étudiée : les cellules ont montré une sensibilité plus faible dans BIOMIMESYS® Brain qu’en 2 dimensions (2D). Cette différence a pu être expliquée par deux phénomènes : une rétention partielle des molécules toxiques dans la matrice, et un phénotype de neurone dopaminergique moins mature qu’en 2D. L’importance du microenvironnement cellulaire a ensuite été étudiée au travers d’une co-culture de cellules Luhmes et d’astrocytes primaires humains en 2D. Cette co-culture a ensuite été transposée dans la matrice BIOMIMESYS®, formant ainsi un modèle complexe incluant à la fois le microenvironnement glial et le microenvironnement matriciel.En parallèle, l’influence du microenvironnement moléculaire a été étudiée sur les cellules SH-SY5Y, une lignée cellulaire issue d’un neuroblastome, couramment utilisée pour évaluer la neurotoxicité de molécules. Dans cette étude, les 24 principaux milieux décrits dans la littérature pour différencier ces cellules en neurones ont été criblés. Les 3 conditions les plus différenciantes en matière de ralentissement de la prolifération cellulaire et de croissance des neurites ont été sélectionnées : l’acide rétinoïque, la staurosporine, et l’Adénosine Monophosphate cyclique (AMPc) associée à du supplément B21. L’expression de marqueurs protéiques neuronaux et la sensibilité des cellules à des composés de toxicités connues ont été mesurées, en 2D et en 3D dans BIOMIMESYS® Brain. La maturité neuronale et la sensibilité aux composés neurotoxiques différaient selon le milieu, en étant les plus hautes en milieu B21+AMPc. La culture en 3D modifiait aussi la réponse des cellules, avec une sensibilité plus faible comparée aux cellules cultivées en 2D.Cette thèse a mis en évidence que le microenvironnement des neurones, qui inclut la MEC, les cellules gliales et les facteurs solubles, modifie la réponse neuronale in vitro et devrait par conséquent être considéré avec attention dans la recherche académique comme industrielle, dès les étapes de criblage de nouveaux médicaments
About 90% of drug candidates fail in clinical trials, for efficacy- and toxicity-related reasons, which often involve the Central Nervous System (CNS). This high failure rate highlights a lack of relevance in experimental models used upstream, including human in vitro models. Indeed, they do not take into account the complexity of the CNS, in which neurons are organized in 3 dimensions (3D) and interact with their microenvironment, composed of cells, soluble factors and extracellular matrix (ECM). The objectives of this PhD were i) to study the influence of these three microenvironment components on neuronal cells in cerebral in vitro models by automatized cellular imaging, and ii) to develop more relevant cerebral in vitro models for phenotypic screening, to assess neurotoxic or therapeutic effects, in the frame of Parkinson’s Disease (PD).First, the BIOMIMESYS® Brain technology has been developed. This acid hyaluronic based-matrix allows the simulation of the ECM and a 3D culture of cerebral cells in 96-well plates. The sensitivity of Luhmes cells, a dopaminergic neuronal cell line, to PD inducers has been studied: the cells displayed a lower sensitivity in BIOMIMESYS® Brain compared to cells cultured in 2 dimensions (2D). This difference was explained by two phenomena: a partial retention of toxic molecules in the matrix, and a lower neuronal maturity compared to cells cultured in 2D.The importance of the cellular microenvironment has been studied through a co-culture of Luhmes cells and primary human astrocytes in 2D. This co-culture has then been transposed in BIOMIMESYS® matrix, to form a complex model including both the glial and the matricial microenvironments.In parallel, the influence of the molecular microenvironment has been studied on the SH-SY5Y cells, a cell line derived from a neuroblastoma, commonly used for neurotoxicity assessment. In this study, the 24 major differentiation media described in the literature to differentiate these cells into neurons have been screened. The 3 most differentiating conditions in terms of proliferation slowdown and neurite elongation have been selected: retinoic acid, staurosporine, and cyclic Adenosine Monophosphate (cAMP) combined to B21 supplement. The neuronal protein marker expression and the cell sensitivity to compounds of known-toxicity have been measured, in 2D and in 3D in BIOMIMESYS® Brain. Both maturity and sensitivity of these neurons varied according to the differentiation medium, and were higher in B21+cAMP. The 3D cell culture modified also the cell response, with a lower sensitivity of cells cultured in 2D.This PhD highlighted that the microenvironment of neurons, including the ECM, the glial cells and the soluble factors, can modify the neuronal response in vitro, and should thus be considered carefully in academic research and as early as possible in the drug discovery industrial process
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Schmid, Jakob [Verfasser], and Matthias [Akademischer Betreuer] Schieker. "A platform for oxygen-controlled cultivation and investigation of 3-dimensional cell cultures for bone tissue engineering / Jakob Schmid ; Betreuer: Matthias Schieker." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1216039321/34.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Waters, John. "3-dimensional culture of endothelial and mural cells allowing interrogation of the role of TGF[beta]/BMP7 signaling in human glomerular endothelial and mesangial cells in glomerulosclerosis." Thesis, University of Cambridge, 2015. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708954.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Cullen, Daniel Kacy. "Traumatically-Induced Degeneration and Reactive Astrogliosis in 3-D Neural Co-Cultures: Factors Influencing Neural Stem Cell Survival and Integration." Diss., Georgia Institute of Technology, 2005. http://hdl.handle.net/1853/7584.

Повний текст джерела
Анотація:
Traumatic brain injury (TBI) results from a physical insult to the head and often results in temporary or permanent brain dysfunction. However, the cellular pathology remains poorly understood and there are currently no clinically effective treatments. The overall goal of this work was to develop and characterize a novel three-dimensional (3-D) in vitro paradigm of neural trauma integrating a robust 3-D neural co-culture system and a well-defined biomechanical input representative of clinical TBI. Specifically, a novel 3-D neuronal-astrocytic co-culture system was characterized, establishing parameters resulting in the growth and vitality of mature 3-D networks, potentially providing enhanced physiological relevance and providing an experimental platform for the mechanistic study of neurobiological phenomena. Furthermore, an electromechanical device was developed that is capable of subjecting 3-D cell-containing matrices to a defined mechanical insult, with a predicted strain manifestation at the cellular level. Following independent development and validation, these novel 3-D neural cell and mechanical trauma paradigms were used in combination to develop a mechanically-induced model of neural degeneration and reactive astrogliosis. This in vitro surrogate model of neural degeneration and reactive astrogliosis was then exploited to assess factors influencing neural stem cell (NSC) survival and integration upon delivery to this environment, revealing that specific factors in an injured environment were detrimental to NSC survival. This work has developed enabling technologies for the in vitro study of neurobiological phenomena and responses to injury, and may aid in elucidating the complex biochemical cascades that occur after a traumatic insult. Furthermore, the novel paradigm developed here may provide a powerful experimental framework for improving treatment strategies following neural trauma, and therefore serve as a valid pre-animal test-bed.
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Choudhury, Sarah F. "An investigation of mechanisms responsible for modulated biosynthetic function in 3-dimensional cultures of a human hepatocyte cell line, for potential use in a bioartificial liver support system." Thesis, University College London (University of London), 2004. http://discovery.ucl.ac.uk/1446890/.

Повний текст джерела
Анотація:
The thesis describes the performance of the HepG2 cell line, a proliferating liver cell line, potentially to be used as the cellular component of a bioartificial liver (BAL). HepG2 cells in 3-Dimensional (3-D) spheroidal culture demonstrate dramatically improved function, compared to monolayer culture. The main aims of the thesis were to investigate the phenomenon in this culture system, whereby function in 3-D culture is optimal between Days 8-10, and thereafter there is a decline in function in spite of continued proliferation and viability. For optimal use in a BAL, it is necessary to increase function per cell either at later time points of 3-D HepG2 culture when cell numbers are greater, and/or increase cell number at times of peak function i.e. Days 8-10 of 3-D culture. The initial hypothesis was that the downregulation of activity observed from Day 11 occurred at the transcriptional level. Approaches to determine expression of liver-enriched transcription factors at times of peak and diminished function in 3-D culture and in monolayer culture were not conclusive. To understand the mechanisms underlying the temporal change in function in 3-D culture, the hypothesis that hypoxia and/or other forms of stress were responsible for the drop in function observed was explored. Microarray data and Western blot analysis highlighted the expression of genes and proteins known to be upregulated by hypoxia at Day 15. Ambient oxygen concentration was increased to attempt to increase cell performance but was ineffective. Genes and proteins implicated in oxidative stress were expressed. Results from assays to measure oxidative stress in HepG2 culture demonstrated an increase at Day 15 compared with Day 8 spheroidal cultures. Attempts to alleviate this stress by supplementing the culture medium with additional anti-oxidants at later times of 3-D culture did not enhance cell performance. Some stress-related proteins and genes were more strongly expressed in Day 8 cultures, as an adaptive response to increased metabolic activity during peak function, while others in Day 15 were turned off. These genes were investigated further at a functional level and results reflected the pattern observed. 3-D culture was manipulated with the addition of extracellular matrix (ECM) in order to enhance cell performance. Cell proliferation was measured by total nuclei quantification, incorporation of BrdU as a measure of DNA synthesis and Ki-67 labelling, as a measure of the total growth fraction. Positively labelled cells were seen throughout the spheroid indicating that even at the centre of the spheroids; there was maintenance not only of viability, but also the capacity to proliferate, indicating other microenvironmental changes may be responsible for the diminished function observed at Day 15. The thesis has highlighted the possible causes for the downregulation of function observed and modulation of the 3-D environment to overcome this, and emphasised the complex relationship between cell performance and the stress response, in order to improve a system which could provide the basis for the biological component of a BAL.
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Song-BinHuang and 黃菘斌. "Development of microfluidic systems for micro-scale animal cell culture- from cell separation, microencapsulation, micro-dispensing to perfusion 3-dimensional cell culture." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/55014334589213623262.

Повний текст джерела
Анотація:
博士
國立成功大學
工程科學系碩博士班
98
Cell-based assays have been widely utilized in life science-related area to quantitatively investigate the link between the cellular responses and the tested conditions for decades. Conventional cell handling techniques mainly involve the cell isolation, separation, immobilization, liquid dispensing, and cell culture practice. These operations, however, might not be able to deal well with the biological sample with a small size. In addition, the commonly-used cell culture protocols might consume more experimental research resources (e.g. number of cells), and therefore the throughput of a cell-based assay might be compromised. More importantly, traditional cell cultures could not provide a stable, well-defined, and physiologically-meaningful culture conditions for cell-based assays due to the design of cell culture format. During the past decade, there have been tremendous advances in microfluidics. Due to the significant differences in several physical phenomena between microscale and macroscale devices, microfluidic technology provides unique functionality, which is not previously possible by using traditional techniques. This study reports several new microfluidic devices for high-performance cell handling and for high-throughput cell culture. All these devices fabricated based on a computer numerical controlled (CNC) milling or SU-8 lithography process for molds and polydimethylsiloxane (PDMS) replica molding processes. Firstly, to achieve cell isolation and separation, a new microfluidic-based filter was presented. The filtration separation mechanism is based on the pneumatically tunable deformation of PDMS membranes, which block the fluid channel with a varied degree. This defines the dimensions of the remaining passageway of fluid channel and thus the passage of the microbeads/cells with a specific size. Because of the miniaturization and tunable characteristics of separation performance, not only is the proposed device applicable to perform cell separation under the circumstance that either harvested specimen is limited to the cell content in a sample is sparse, but it also paves a new rout to separate/isolate cells in a simple, controllable and cell-friendly manner. To immobilize cells for 3-D cell culture purpose, a new microfluidic device for continuous generation of alginate microbeads was proposed. The working mechanism is based on the use of a pneumatically-driven vibrator to continuously spot tiny alginate microdroplets in a thin oil layer. The temporarily formed alginate microdroplets are soon sinking into a sterile calcium chloride solution to become gelled microbeads. By regulating the alginate suspension flow rate and the pulsation frequency of the integrated vibrator, the alginate microbeads can be produced in a size-controllable manner. Furthermore, a microfluidic-based pneumatically-driven micro-dispenser was demonstrated for precise pipetting of sub-microliter samples. The key feature of the micro-dispenser is the use of a suction membrane to provide a driving force for precise and quick aqueous liquid sampling and pipetting. The micro-dispenser features in the elegant control of the releasing time of the air pressure in the pneumatic chamber of the pressure-generating unit, contributing to precise pipetting of aqueous liquid volumes ranging from 0.05 μl to 0.45 μl (the minimum unit is 0.05 μl) achieving the multi-volume dispensing capability. By means of proper combinations, the liquid of various volumes would be easily sampled. In addition, a new perfusion-based, micro three-dimensional (3-D) cell culture platform was proposed for high-throughput bioassays using enabling microfluidic technologies. The main characteristics of the chip are the capability of multiple medium deliveries without any back-flow by using the new design pneumatic C-shape micropumps, and the function of efficient cells/hydrogel scaffold loading. Based on the inherent natures of miniaturized perfusion 3-D cell culture, the cell culture chip not only can provide stable, well-defined and more biologically-relevant culture environments, but also features in low consumption of research resource. All these traits are found particularly useful for high-precision and high-throughput 3-D cell culture-based assays. Finally, all the microfluidic devices proposed in the research were demonstrated to perform the process including separation, microencapsulation of the chondrocytes and investigation the effect of extracellular pH on chondrocyte functions. Experimental results showed that the chondrocytes from the limited enzymatically-digested tissue suspension can be successfully separated by using the microfluidic-based filter with an excellent cell separation efficiency of 93 % and a high cell viability of 96%. Moreover, the separated chondrocytes were encapsulated in alginate microbeads with high cell viability (94±2%) by using the microfluidic alginate microbead generator. Besides, a micro-scale perfusion 3-D cell culture-based assay to study the effect of extracellular pH on chondrocyte was successfully demonstrated using the proposed cell culture chip and the micro-dispenser was used to adjust the different pH value of the medium. The results were also compared with the same evaluation based on conventional static cell culture with larger culture scale. As a whole, these microfluidic systems proposed in the study provide a simple, automatic, controllable, uniform, cell friendly, less contaminated manner for cell manipulation and culturing and may facilitate a high-throughput cell culture based assay in the more in vivo-like environment.
Стилі APA, Harvard, Vancouver, ISO та ін.
Більше джерел

Книги з теми "3-dimensional cell culture"

1

United States. National Aeronautics and Space Administration., ed. The use of microgravity to emulate three-dimensional tissue interactions in colorectal cancer metastasis: Grant #: NAG 9-650, Period: 3-1-93 to 2-28-97, inclusive. [Washington, DC: National Aeronautics and Space Administration, 1997.

Знайти повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Частини книг з теми "3-dimensional cell culture"

1

Dhanya, K. C., and Aditya Menon. "3 Dimensional Cell Culture Techniques in Cancer Research." In Pharmacotherapeutic Botanicals for Cancer Chemoprevention, 283–98. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5999-0_11.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Pavlovic, Mirjana. "Cell Culture in Bioengineering-Working on 3-Dimensional Culture and Ink-Jet Printing: Regenerative Medicine (RM)." In Bioengineering, 281–88. Cham: Springer International Publishing, 2014. http://dx.doi.org/10.1007/978-3-319-10798-1_21.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Korff, Thomas. "Three-Dimensional Spheroid Culture of Endothelial Cells." In Methods in Endothelial Cell Biology, 55–62. Berlin, Heidelberg: Springer Berlin Heidelberg, 2004. http://dx.doi.org/10.1007/978-3-642-18725-4_6.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Lang, E., and K. Maier. "Cytological Characterization of Three-dimensional, Graftable Human Keratinocyte Cultures." In Cell and Tissue Culture Models in Dermatological Research, 118–25. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77817-9_13.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Bittinger, F., C. L. Klein, C. Skarke, C. Brochhausen, M. Otto, H. Köhler, and C. J. Kirkpatrick. "A Three-Dimensional Cell Culture Method for Studying Peritoneal Adhesions." In Peritoneal Adhesions, 49–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60433-1_6.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
6

Konno, Ken-ichi, Tadashi Kosawada, Ryota Sato, Zhonggang Feng, Yasukazu Hozumi, and Kaoru Goto. "Three-Dimensional Micro Vibration Stage and Its Application to Cell Culture." In IFMBE Proceedings, 1409–12. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-642-14515-5_359.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Napiwocki, Brett N., Alana Stempien, Jacob Notbohm, Randolph S. Ashton, and Wendy Crone. "Two-Dimensional Culture Systems to Investigate Mechanical Interactions of the Cell." In Mechanics of Biological Systems, Materials and other topics in Experimental and Applied Mechanics, Volume 4, 37–39. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-63552-1_6.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Herrmann, K., V. Kunzelmann, M. Bruns, and U. F. Haustein. "Cytokine-dependent Regulation of Human Dermal Fibroblasts Cultured in a Three-dimensional Collagen-Gel." In Cell and Tissue Culture Models in Dermatological Research, 230–36. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-77817-9_25.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Asaoka, K. "Characterization of Bombyx and Related Insect Cell Lines by SDS-Page and Two-Dimensional Gel Electrophoresis." In Invertebrate and Fish Tissue Culture, 278–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73626-1_66.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Franqui, Lidiane Silva, Luis Augusto Visani de Luna, Thomas Loret, Diego Stefani Teodoro Martinez, and Cyrill Bussy. "Assessing the Adverse Effects of Two-Dimensional Materials Using Cell Culture-Based Models." In Nanotechnology Characterization Tools for Environment, Health, and Safety, 1–46. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-59600-5_1.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.

Тези доповідей конференцій з теми "3-dimensional cell culture"

1

Huang, Song-Bin, Min-Hsien Wu, Zhanfeng Cui, Zheng Cui, and Gwo-Bin Lee. "Microdfluidic Based 3-Dimensional Cell Culture Platform." In ASME 2008 First International Conference on Micro/Nanoscale Heat Transfer. ASMEDC, 2008. http://dx.doi.org/10.1115/mnht2008-52292.

Повний текст джерела
Анотація:
This study reports a new perfusion-based, micro three-dimensional (3-D) cell culture platform for drug testing using enabling microfluidic technologies. In this work, a perfusion-based, micro 3-D cell culture platform is designed and is fabricated based on SU-8 lithography and polydimethylsiloxane (PDMS) replication processes. One of the key features of the system is that the incorporation of a multiple medium pumping mechanism, consisting of 15 membrane-based pneumatic micropumps with serpentine-shape (S-shape) layout, coupled with a pneumatic tank, into the micro 3-D cell culture platform to provide efficient and economical culture medium delivery. Moreover, a “smart cell/agarose (scaffold) loading mechanism” was proposed, allowing the cell/3-D scaffold loading process in one step and avoiding too much laborious works and manual error. The results show that in all of the 15 S-shape pneumatic micropumps studied, the medium delivery mechanism is able to provide a uniform flow output ranging from 5.5 to 131 μl/hr depending on the applied pulsation frequency of the micropumps. In addition, the cell/agarose (scaffold) loading mechanism was proved to be able to perform sample loading tasks precisely and accurately in all of the 15 microbioreactors integrated. Furthermore, anti-cancer drug testing was successfully demonstrated using the proposed culture platform and fluorescent microscopic observation. As a whole, because of miniaturization, not only does this perfusion 3-D cell culture platform provide a homogenous and steady cell culture environment, but it also reduces the need for human intervention. Moreover, due to the integrated pumping of the medium and the cell/agarose (scaffold) loading mechanisms, time efficient and economical research work can be achieved. These characteristics are found particularly useful for high-precision and high-throughput 3-D cell culture-based drug testing.
Стилі APA, Harvard, Vancouver, ISO та ін.
2

Isu, Giuseppe, Diana Massai, Giulia Cerino, Diego Gallo, Cristina Bignardi, Alberto Audenino, and Umberto Morbiducci. "A Novel Perfusion Bioreactor for 3D Cell Culture in Microgravity Conditions." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14502.

Повний текст джерела
Анотація:
Cell suspension culture methods based on the generation of microgravity environment are widely used in regenerative medicine for (1) the production of native-like three-dimensional (3D) cell aggregates and engineered tissues [1,2,3], for (2) low cost scalable cell expansion and long-term cell viability maintenance [4,5], and for (3) guiding differentiation of stem cells (SCs) [6]. The generation of a microgravity environment for 3D cell cultures, mimicking the native environment, promotes spatial freedom, cell growth, cell-cell interaction and improves mass transfer and cell exposure to nutrients. Nowadays, microgravity cell cultures are obtained by using stirred or rotating bioreactors, but both devices suffer from limitations: stirring bioreactors generate non-physiological shear stresses, which could damage cultured cells, interfere with SC pluripotency, and limit reproducibility of the culture process; rotating bioreactors are expensive devices due to the complex technological solutions adopted for obtaining rotation [5].
Стилі APA, Harvard, Vancouver, ISO та ін.
3

Kin Fong Lei, Min-Hsien Wu, Che-Wei Hsu, and Yi-Dao Chen. "Non-invasive measurement of cell viability in 3-dimensional cell culture construct." In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2013. http://dx.doi.org/10.1109/embc.2013.6609467.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
4

Zou, Xiang-Hui, Dong-Hong Zhuang, Nagahiro Saito, Yun-Ying Wu, Guang-Cai Zha, and Osamu Takai. "A novel 3-dimensional cell culture system for embryoid bodies' formation." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639591.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
5

Lelkes, Peter I., and Brian R. Unsworth. "Cellular Signaling Mechanisms Involved in the 3-Dimensional Assembly and Differentiation of PC12 Pheochromocytoma Cells Under Simulated Microgravity in NASA Rotating Wall Vessel Bioreactors." In ASME 1998 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1998. http://dx.doi.org/10.1115/imece1998-0791.

Повний текст джерела
Анотація:
Abstract Rotating Wall Vessel (RWV) Bioreactors simulate microgravity and facilitate 3-D tissue-like assembly through spatial co-localization and cell-cell interactions. This unique cell culture venue is well suited to assess the role of micro-environmental cues in the assembly and tissue-specific differentiation of cells in culture. Our long term goal is to use RWV Bioreactors for generating functional neuroendocrine 3-D constructs which may be useful as clinical replacement tissue in treating neurodegenerative diseases. As a model we are using PC12 pheochromocytoma cells, a bipotential rat adrenal medullary tumor cell line. PC 12 cells differentiate, depending on exogenous factors, either along the neuronal or the neuroendocrine pathway. PC12 cells, when maintained for up to 20 days in RWV Bioreactors, form macroscopic tissue-like aggregates which exhibit enhanced expression of neuroendocrine, adrenergic differentiation markers (Lelkes et al., In Vitro Devel. Biol, 1998, 34: 316–325). We hypothesized that exposure of PC12 cells to the “simulated microgravity” culture conditions in the RWV Bioreactors, might selectively activate signal transduction pathways leading to enhanced neuroendocrine adrenergic differentiation. Using quantitative RT-PCR we demonstrated rapid upregulation of an adrenergic marker, phenylethanolamine-N-methyl transferase (PNMT), in short term RWV cultures. Concomitantly, we found, by electrophoretic mobility shift assays, differential induction of nuclear transcription factors, such as GRE and SP-1, which are known to be involved in the glucocorticoid-induced activation of PNMT. Conversely, upon short term culture of PC12 cells in RWV, the neuronal traits of the cells were impaired. Upon exposure to simulated microgravity, MAPK signaling (erk and jnk) was constitutively activated, while nerve-growth factor (NGF)-induced activation of erk, was abrogated. These results suggest that the culture conditions in the RWV Bioreactors are sufficient to induce PC12 cell differentiation towards the neuroendocrine, phenotype by upregulating “adrenergic” gene expression, while downregulating neurotrophin signaling pathways.
Стилі APA, Harvard, Vancouver, ISO та ін.
6

George, Subin M., and Hyejin Moon. "Alginate hydrogel based 3-dimensional cell culture and chemical screening platform using digital microfluidics." In 2015 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS). IEEE, 2015. http://dx.doi.org/10.1109/memsys.2015.7050985.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
7

Sepramaniam, Sugunavathi, Xin Hui Chew, Kao Chin Ngeow, and May Ann Lee. "Abstract 2939: ETC159, a porcupine inhibitor, exhibits synergism with PI3K inhibitors in 3-dimensional cell culture." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-2939.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
8

Cevik, Ziysan Buse, Aylin Korkmaz, Nermin Topaloglu Avsar, and Ozan Karaman. "Evaluation Of ATP Production Of Low Level Laser Therapy In Monolayer And 3 Dimensional Cell Culture." In 2021 Medical Technologies Congress (TIPTEKNO). IEEE, 2021. http://dx.doi.org/10.1109/tiptekno53239.2021.9632911.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
9

Muguruma, Masako, Saeko Teraoka, Kana Miyahara, Ai Ueda, Takahiko Kawate, and Takashi Ishikawa. "Abstract 326: Differences of drug sensitivities between 2-dimensional and 3-dimensional culture systems in triple negative breast cancer cell lines." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-326.

Повний текст джерела
Стилі APA, Harvard, Vancouver, ISO та ін.
10

Takagi, Yuta, Toshihiko Shiraishi, Shin Morishita, Ryohei Takeuchi, Tomoyuki Saito, and Yuko Mikuni-Takagaki. "Effects of Mechanical Vibration on Matrix Production and Proliferation of Three-Dimensional Cultured Chondrocytes." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66805.

Повний текст джерела
Анотація:
This paper describes the effects of vibration stimulation on chondrocytes in three-dimensional culture in relation to the production of regenerative cartilage tissue, using collagen artificial skin as a carrier and supplementation with hyaluronic acid (used in the conservative treatment of osteoarthritis), and the mechanism of the adaptive response of chondrocytes to mechanical loading. The experimental condition imitates an environment of articular cartilage in vivo that chondrocytes are completely surrounded by the extracellular matrix and receives mechanical stimulation for the weight-bearing mechanics. Chondrocytes were isolated from articular cartilage of porcine metatarsophalangeal joints. Experiments were performed under four different culture conditions: control condition, in which chondrocytes were cultured with atelocollagen gel and collagen artificial skins, and no vibration (HA−Vib−); HA−Vib+, in which chondrocytes were cultured in atelocollagen gel and collagen artificial skins with vibration treatment for 2 weeks; HA+Vib−, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid; and HA+Vib+, in which chondrocytes were cultured in medium containing 0.1% hyaluronic acid with vibration treatment for 2 weeks. Histologic analysis was conducted at 14 days of culture. The proliferation of chondrocytes was obtained by counting the number of cells with a hemocytometer after 3, 7, 10, and 14 days of culture. The expression of Sox 9 and β-catenin was detected by western blotting analysis. Sox 9 has been reported of involvement in transcription of type IX collagen that binds cartilage-specific type II collagen fibrils. β-catenin plays an important role of signaling pathways of cell proliferation although the relationship between β-catenin and mechanical vibration stimulation has not been clarified yet. The obtained results are as follows. The mechanical vibration enhanced the thickness of extracellular matrix of chondrocytes in histologic section at 14 days of culture and increased the expression of Sox 9. In addition, the mechanical vibration significantly increased the number of chondrocytes after 10 days of culture and promoted the expression of β-catenin. These results show that mechanical vibration promotes the matrix production and proliferation of chondrocytes and that a part of important signaling pathways in relation to mechanical vibration stimulation and proliferation of chondrocytes has been revealed.
Стилі APA, Harvard, Vancouver, ISO та ін.
Також вас можуть зацікавити розширені добірки на тему "3-dimensional cell culture" для конкретних типів джерел:
Статті в журналах Дисертації
Ми пропонуємо знижки на всі преміум-плани для авторів, чиї праці увійшли до тематичних добірок літератури. Зв'яжіться з нами, щоб отримати унікальний промокод!

До бібліографії