Дисертації з теми "2D MS"
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Joyner, Jeffrey Clark. "The Use of 2D-LC-MS/MS in disease characterization and global proteomics." Connect to resource, 2006. http://hdl.handle.net/1811/6604.
Повний текст джерелаTitle from first page of PDF file. Document formatted into pages: contains 46 p.; also includes graphics. Includes bibliographical references (p. 46). Available online via Ohio State University's Knowledge Bank.
Vieira, José Cavalcante Souza. "Investigação de metalotioneínas em peixes da região de Jirau - bacia do Rio Madeira - Rondônia." Botucatu, 2017. http://hdl.handle.net/11449/150736.
Повний текст джерелаResumo: Devido a sua grande concentração de nutrientes, tais como proteínas, vitaminas e minerais, o peixe é considerado um dos alimentos mais saudáveis que se pode encontrar na natureza. No entanto, a ingestão de peixes é considerada a forma predominante de via de exposição do ser humano ao mercúrio (Hg), principalmente para as populações que vivem às margens dos rios, onde o peixe constitui a principal fonte de proteína. Na tentativa de elucidar os mecanismos de toxicidade das espécies mercuriais, o teor desse metal tem sido estudado intensamente pela comunidade científica nas últimas décadas em amostras de solo, sedimentos, humanos e peixes na Amazônia brasileira. Sabe-se que as espécies mercuriais bioacumuladas nos tecidos dos seres vivos ligam-se a metaloproteínas, e quando há uma concentração alta de metal tóxico nos organismos, esses passam a expressar proteínas de defesa, denominadas metalotioneínas (MTs) responsáveis pelo transporte e eliminação de metais tóxicos. Apesar de estudos mostrarem o aumento das metalotioneínas em animais expostos a metais potencialmente tóxicos, essas proteínas não foram caracterizadas para confirmação de sua veridicidade, são analisadas por métodos indiretos, esse fato leva a necessidade de técnicas mais precisas na identificação de metalotioneínas. Levando em consideração o exposto esse estudo teve como objetivo otimizar métodos de quantificação de mercúrio e técnica de eletroforese para identificação de possíveis metalotioneínas biomarcadoras d... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Due to its high concentration of nutrients, such as proteins, vitamins and minerals, fish is considered one of the healthiest foods that one can find in nature. However, fish intake is considered to be the predominant human exposure pathway to mercury (Hg), especially for populations living along riverbanks where fish are the main source of protein. In the attempt to elucidate the toxicity mechanisms of mercurial species, the content of this metal has been intensively studied by the scientific community in recent decades in soil, sediment, human and fish samples in the Brazilian Amazon. It is known that mercurial species bioaccumulated in the tissues of living beings bind to metalloproteins, and when there is a high concentration of toxic metal in organisms, they begin to express defense proteins, called metallothioneins (MTs) responsible for the transport and elimination of Toxic metals. Although studies have shown the increase of metallothioneins in animals exposed to potentially toxic metals, these proteins have not been characterized to confirm their veridicity, are analyzed by indirect methods, this fact leads to the need for more precise techniques in the identification of metallothioneins. Taking into account the above, this study aimed to optimize mercury quantification methods and electrophoresis technique for identification of possible mercury biomarkers metallothionein in muscular and hepatic tissue of fish of economic interest, Tucunaré (Cichla spp.), Filhote (Bra... (Complete abstract click electronic access below)
Doutor
Bittarello, Alis Correia. "Estudo de biomarcadores de mercúrio em peixes da amazônia por meio da metalômica e análise do estresse oxidativo." Botucatu, 2017. http://hdl.handle.net/11449/151174.
Повний текст джерелаResumo: O mercúrio é um metal tóxico, de distribuição ubíqua, com capacidade para bioacumulação e biomagnificação, que provoca alterações em biomoléculas importantes no metabolismo e contribui para o estabelecimento do estresse oxidativo em organismos aquáticos. Logo, o presente estudo teve por objetivo identificar e avaliar possíveis biomarcadores proteicos e/ou enzimáticos da toxicidade do mercúrio em peixes da região amazônica, por meio do estudo metaloproteômico e avaliação do estresse oxidativo. Foram utilizadas metodologias de fracionamento e identificação de proteínas por eletroforese bidimensional (2D PAGE) associada à espectrometria de massas (MS), mapeamento do mercúrio, em spots proteicos, por espectrometria de absorção atômica em forno de grafite (GFAAS) e avaliação de marcadores de estresse oxidativo. As espécies utilizadas foram o Plagioscion squamosissimus (corvina) e Colossoma macropomum (tambaqui), coletados na área da Usina Hidrelétrica de Jirau (rio Madeira-RO), que foram selecionadas em função da abundância populacional, interesse para a pesca e posição diferente na cadeia trófica (carnívoro e onívoro, respectivamente). Os tecidos amostrados foram o hepático, renal e muscular. Os resultados obtidos demonstraram maior concentração de mercúrio total no P. squamosissimus, espécie carnívora, e padrão de distribuição deste elemento igual para ambas as espécies (fígado>rim>músculo). Há tendência para maior atividade enzimática nos tecidos hepático e renal da espécie com... (Resumo completo, clicar acesso eletrônico abaixo)
Doutor
Nakata, Michael Takeshi. "Simulating the FTICR-MS Signal of a Decaying Beryllium-7 Ion Plasma in a 2D Electrostatic PIC Code." Diss., CLICK HERE for online access, 2010. http://contentdm.lib.byu.edu/ETD/image/etd3370.pdf.
Повний текст джерелаEckberg, Melanie N. "Forensic Toxicological Screening and Confirmation of 800+ Novel Psychoactive Substances by LC-QTOF-MS and 2D-LC Analysis." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3923.
Повний текст джерелаNiranjane, Ajay Pundaiikrao, and ajay niranjane@gmail com. "Screening diverse cellulase enzymes from the white rot fungus Phlebia gigantea for high activity and large scale applications." RMIT University. Applied Sciences, 2006. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080513.150257.
Повний текст джерелаHalienová, Andrea. "Změny proteomu a metabolomu u vybraných organismů ve stresových podmínkách." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-233313.
Повний текст джерелаHoward, James W. "The development of mass spectrometry-based methodologies for the high throughput quantitation of peptides in biological matrices." Thesis, Loughborough University, 2018. https://dspace.lboro.ac.uk/2134/32454.
Повний текст джерелаAnastacio, Amandine. "Etude du profil protéomique de follicules ovariens de souris à 3 différents stades de développement in vitro." Phd thesis, Université Pierre et Marie Curie - Paris VI, 2014. http://tel.archives-ouvertes.fr/tel-00990894.
Повний текст джерелаŠopíková, Martina. "Změny proteinového profilu v průběhu sladování ječmene." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216437.
Повний текст джерелаNgara, Rudo. "A proteomic analysis of drought and salt stress responsive proteins of different sorghum varieties." Thesis, University of the Western Cape, 2009. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_1434_1334579378.
Повний текст джерелаThis study reports on a proteomic analysis of sorghum proteomes in response to salt and hyperosmotic stresses. Two-dimensional gel electrophoresis (2DE) in combination with mass spectrometry (MS) was used to separate, visualise and identify sorghum proteins using both sorghum cell suspension cultures and whole plants. The sorghum cell suspension culture system was used as a source of culture filtrate (CF) proteins. Of the 25 visualised CBB stained CF spots, 15 abundant and well-resolved spots were selected for identification using a combination of MALDI-TOF and MALDI-TOFTOF MS, and database searching. Of these spots, 14 were positively identified as peroxidases, germin proteins, oxalate oxidases and alpha-galactosidases with known functions in signalling processes, defense mechanisms and cell wall metabolism.
Fertin, Marie. "Recherche de biomarqueurs circulants du remodelage ventriculaire gauche en post-infarctus du myocarde." Thesis, Lille 2, 2012. http://www.theses.fr/2012LIL2S025.
Повний текст джерелаLeft ventricular (LV) remodelling after myocardial infarction (MI) indicates a high risk of heart failure and death but remains difficult to predict in clinical practice. Biomarkers may help to refine risk stratification. The main purpose was to find circulating biomarkers of LV remodelling after MI, using two strategies : candidate protein approach and differential proteomic approach, working on a population with a clearly defined phenotype, the REVE-2 study, a prospective multicenter study including 246 patients with a first anterior Q-wave MI. Blood samples were obtained at hospital discharge, at 1 month, 3 months and 1 year. An echocardiography was performed at the same time except for the 1st month to assess LVR.By candidate protein approach, we confirmed that B-type natriuretic peptide (BNP) was a powerful predictor of LV remodelling after MI. Additional biomarkers, such as matrix metalloproteinase-8 (MMP-8), MMP-9, hepatocyte growth factor (HGF), C-reactive protein (CRP) and cardiac troponin I were found to be associated with LV remodelling, highlighting several pathways implicated in pathophysiology of LV remodelling. We have also shown that biomarkers in association (BNP and cardiac troponin I, BNP and MMP-8, BNP and MMP-9) could improve risk stratification in post-MI by selecting groups of patients at higher risk.As the ideal biomarker was still not identified, we applied a differential proteomic approach, with no a priori hypothesis, in order to characterize proteomic signature of LV remodelling. The use of a protein enrichment kit, consisting of a library of combinatorial hexapeptide ligands, compressed the protein concentration range of plasma and serum, through the simultaneous onestep dilution of high-abundance and concentration of lowabundance proteins. Protein enrichment kit prior to two-dimensional (2D) electrophoresis or SELDI TOF MS (surface-enhanced laser desorption–ionization time of flight) analysis enabled the detection of proteins that were not detected in native blood sample and the accessibility to proteolytic fragments obtained from major proteins. Clusterin (apolipoprotein J) was identified as a potential biomarker of LV remodelling by 2D-DIfferential Gel Electrophoresis (2D-DIGE). Clusterin was quantified by Western blot and ELISA and was found to be positively associated with LV remodelling. However, this association was not found with all LV remodelling parameters nor at each time during the year following MI, requiring further analysis. Differential proteomic approach by SELDI TOF MS selected 26 m/z peaks, as potential biomarkers of LV remodelling. Of them, 12 were identified by mass spectrometry. The 2777 m/z peak was identified directly from the ProteinChip array as being the N-terminal peptide (24–48 aa) generated from albumin by pepsin cleavage. Other peaks were identified after purification using chromatographic columns or liquid-phase isoelectric focusing : most of them were found to be proteolytic fragments of proteins like fibrinogen, C3, C4 and C1q complement. Identifications have now to be validated with specific techniques, usually by immmunoprecipitation and Western blot analysis.Finding new biomarkers of LV remodelling could help refine risk stratification and identify patients in whom more aggressive therapy and/or more frequent follow-up could be needed
Magalhães, Ilídio Miguel Teixeira. "Proteome of biofilm produced by a S. pseudintermedius strain." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14291.
Повний текст джерелаStaphylococcus pseudintermedius is an opportunistic pathogenic bacterium responsible for most skin and post-surgical infections in dogs. The number of bacterial strains resistant to β-lactam antibiotics is increasing and are the major challenges now faced by veterinary medicine. Bacteria that produce biofilm are more resistant to treatment and thus, the production of this structure is already considered a virulence factor. In a biofilm, bacteria are embedded in a matrix of extracellular polymeric substances (EPS) some of which are proteins. With the objective to know more of this array element, the characterization of the biofilm matrix proteome (BMP) from a highly virulent S. pseudintermedius strain isolated from a dog with severe pyoderma was performed. Biofilm was developed by culturing the S. pseudintermedius strain 5819/10 in specific media. The biofilm matrix was then be separated from bacterial cells and evaluated for their protein content and complexity. Finally, the proteome was separated by 1D electrophoresis and characterized by nanoLC-ESI-Q-TOF and analysed using bioinformatics tools. The BMP of strain S. pseudintermedius 5819/10 consisted in a diverse group of proteins, where 63% of the proteins could be related to either the extracellular region or the plasma membrane, as protein complexes, and most of them had functions essential to cell survival. However, it was not possible to establish a clear relation between them and biofilm formation. Proteins known to be involved in biofilm formation consisted mostly of regulator factors of biofilm formation as well as virulence factors of-mainly-bacterial cell adhesion and host colonization. The prevalence of adhesins and the almost total absence of proteins involved in EPS synthesis pointed to a biofilm matrix where cells are directly or indirectly closely glued together to each other.
Staphylococcus pseudintermedius (S.pseudintermedius) é uma bactéria patogénica oportunista, responsável pela maioria das infeções cutâneas e pós-cirúrgicas em cães. O número de estirpes resistentes a antibióticos β-lactâmicos está a aumentar constituindo actualmente um dos grandes desafios enfrentados pela medicina veterinária. As bactérias mais resistentes ao tratamento são aquelas que produzem biofilme sendo esta capacidade considerada um fator de virulência. Num biofilme, as bactérias estão envoltas numa matriz de substâncias poliméricas extracelulares (SPE), algumas das quais são proteínas. Tendo por objectivo obter mais informação acerca do biofilme, foi caracterizado o proteoma da matriz do biofilme de uma estirpe bastante virulenta de S. pseudintermedius isolada de um cão com piodermite profunda. Para tal cultivaram-se biofilmes da estirpe de S. pseudintermedius 5819/10 em meio apropriado, separou-se a matriz das suas células bacterianas e avaliou-se as proteínas presentes quanto ao seu conteúdo e complexidade. Posteriormente o proteoma foi separado por electroforese 1D, caracterizado por nanoLC-ESI-Q-TOF e analisado usando ferramentas bioinformáticas Constatou-se que o proteoma da matriz do biofilme da estirpe 5819/10 de S. pseudintermedius é muito diverso e que 63% das proteinas podem estar relacionadas com a região extracelular do biofilme ou da membrana plasmática na forma de complexos proteicos. Verificou-se também que a maioria das proteínas identificadas possui funções essenciais para a sobrevivência da bactéria mas não foi possível estabelecer uma relação clara entre elas e a formação de biofilmes. Algumas proteínas que se sabe estarem envolvidas na formação de biofilmes foram identificadas, tratam-se principalmente de factores reguladores da formação de biofilme e outros factores de virulência relacionados com a colonização de um hospedeiro a adesão bacteriana a uma superfície. A prevalência de adesinas e a ausência quase total de proteínas envolvidas na síntese de SPEs, forneceu dados que apoiam a hipótese que a matriz do biofilme do S. pseudintermedius 5819/10 seja constituída por células directamente ou indirectamente unidas entre si.
Kouloura, Eirini. "Phytochemical investigation of Acronychia species using NMR and LC-MS based dereplication and metabolomics approaches." Thesis, Paris 5, 2014. http://www.theses.fr/2014PA05P636/document.
Повний текст джерелаMedicinal plants constitute an unfailing source of compounds (natural products – NPs) utilised in medicine for the prevention and treatment of various deceases. The introduction of new technologies and methods in the field of natural products chemistry enabled the development of high throughput methodologies for the chemical composition determination of plant extracts, evaluation of their properties and the exploration of their potentials as drug candidates. Lately, metabolomics, an integrated approach incorporating the advantages of modern analytical technologies and the power of bioinformatics has been proven an efficient tool in systems biology. In particular, the application of metabolomics for the discovery of new bioactive compounds constitutes an emerging field in natural products chemistry. In this context, Acronychia genus of Rutaceae family was selected based on its well-known traditional use as antimicrobial, antipyretic, antispasmodic and anti-inflammatory therapeutic agent. Modern chromatographic, spectrometric and spectroscopic methods were utilised for the exploration of their metabolite content following three basic axes constituting the three chapters of this thesis. Briefly, the first chapter describes the phytochemical investigation of Acronychia pedunculata, the identification of secondary metabolites contained in this species and evaluation of their biological properties. The second chapter refers to the development of analytical methods for the identification of acetophenones (chemotaxonomic markers of the genus) and to the dereplication strategies for the chemical characterisation of extracts by UHPLC-HRMSn. The third chapter focuses on the application of metabolomic methodologies (LC-MS & NMR) for comparative analysis (between different species, origins, organs), chemotaxonomic studies (between species) and compound-activity correlations
Miranda, Helder. "Stress response in the cyanobacterium Synechocystis sp. PCC 6803." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43086.
Повний текст джерелаHarju, Mikael. "Analysis of PCBs with special emphasis on comprehensive two-dimensional gas chromatography of atropisomers." Doctoral thesis, Umeå universitet, Kemi, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-54.
Повний текст джерелаShiryaeva, Liudmila. "Proteomics and metabolomics in biological and medical applications." Doctoral thesis, Umeå universitet, Kemiska institutionen, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-43520.
Повний текст джерелаSamuel, Jacob Matthew. "Development of methods for the analysis of human protamine via 2D LC-MS/MS." Thesis, 2018. https://hdl.handle.net/2144/33039.
Повний текст джерелаKuo, Yung-Yu, and 郭永宇. "Profiling novel urinary markers for prostate cancer by 2D HPLC-ESI-MS." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/01842560567854299065.
Повний текст джерела臺灣大學
化學研究所
98
Prostate cancer ranks the seventh cause of cancer-related death in Taiwan and the second in United States. The incidence and mortality rates of prostate cancer have been rising rapidly in the past decades. Currently, there are no accurate and specific biomarkers which could discriminate clinically relevant from clinically benign disease. The better indicators and progression are needed to avoid unnecessary treatment. Clinically, prostate-specific antigen (PSA) screening still seems the only way to diagnose the carcinoma of prostate, but lately researches showed that PSA is not prostate cancer specific. Finding novel biomarkers that are sensitive, specific and can be examined by non-invasive means are imperative. In this thesis, the on-line 2D LC-MS and off-line 2D LC-MS methods are the main approaches to unravel the protein profiling information from urine samples. In order to acquire the moderate experimental results and establish the complete urine sample analysis for identifying prostate cancer, we use these two approaches and tested the effects of modifying several parameters (oven temperature, mobile phase gradient, salt concentration and so on). In online cases, though it can be automatically operated, the information obtained turned out to be unsatisfactory. In offline cases, it demanded much manpower for sample preparation and operating the LC-MS system. The results in offline cases not only show higher intensity and sensitivity than online cases, but provide more useful information.
Lee, Wei-Han, and 李威漢. "The Development of On-line Single / Staggered Multi-Step Elution SPE-CE-MS and Heart-cut 2D–CE-MS." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/36753382916516650744.
Повний текст джерела臺灣大學
化學研究所
98
A PDMS based two-leveled two cross design interface was proposed for on-line coupling SPE-CE-MS. In this interface, the SPE column and the CE separation column were positioned orthogonally and two crosses were fabricated on the interface. With the two cross design, the operation of SPE could be performed independently without unexpected flow through leakage into the separation column. The performance of the interface was optimized using a peptide mixture. The position of the SPE column related to the CE separation channel was found to be critical to the performance of the system. Under the optimal position, the separation efficiency was similar to a CE-MS experiment without SPE. The peptide signals were enhanced 50 to 100-fold and the repeatability was within 4% RSD for migration time and 10% RSD for peak area. A tryptic digest of cytochrome C was used to demonstrate the feasibility of the interface in protein identification at a level of 1 ng/mL. In a protein mixture analysis, the identification of proteins usually suffers in low sequence coverage in the single run CZE-ESI-MS/MS. An original concept of on-line coupling multistep elution solid phase extraction (SPE) to CZE-MS/MS was proposed to increase sequence coverage of protein mixture analysis. The multistep elution SPE (the first dimension) provides an additional dimension of separation prior to CZE (the second dimension) and extends the separation capacity for protein mixture analysis. Furthermore, a staggered CZE method was described to increase the throughput of each CZE runs in the second dimension separation and thus to reduce entire analysis time. In this study for protein mixture standards, more than 60% of additional peptides were discovered , and more than 50% was improved in sequence coverage by using multistep elution SPE-CE-MS/MS. By using staggered CZE method, half of the entire analysis time could be saved (54%) in comparison with the sequential CZE method used in multistep elution SPE-CE-MS/MS and thus avoiding the time-consuming analytical procedure in comprehensive 2D separation. An interface for heart-cut 2D CE-MS was proposed to increase separation selectivity in mixture analysis. Several concepts were adapted to overcome the limitations of heart-cut 2D-CE designed in the present studies. First, the manipulation of chip-based interface provides an isolated buffer system to connect two sets of capillary electrophoresis. Second, the parallel separation of the two dimensional capillary electrophoresis was detected simultaneously by a pulsed electrospray-based duel-channel CE-MS system. In this study, the system was demonstrated by using capillary zone electrophoresis- micellar electrokinetic chromatography (CZE-MEKC) system to analyze sulfonamide mixtures. Under the consideration of correspondence in EOF for fused silica capillary the PDMS based chip channel, 8 sulfonamide standards can be transferred successfully without loss and peak broadening during the heart-cutting operation. The preliminary feasibility of heart-cut CZE-MEKC with dual-channel CE-MS was studied in sulfonamides analysis. Four sulfonamides(SDZ、SMR、STZ、SMM) were transferred into the MEKC channel by the heart-cut interface after separation in the first dimension of CZE. The migration order of four heart-cut sulfonamides was found similar order in the single-run MEKC.
Mella, Malorie Ann. "Detection of cocaine and its major metabolites in bone following outdoor decomposition after chronic cocaine administration using 2D-LC/MS/MS." Thesis, 2017. https://hdl.handle.net/2144/20788.
Повний текст джерелаForest, Anik. "Évaluation de différentes composantes chromatographiques d'un système nano-LC-MS pour des applications protéomiques." Thèse, 2006. http://hdl.handle.net/1866/17985.
Повний текст джерела-Ju, Chun, and 陳君茹. "1.Post-translational modification on protein by glyoxal and methylglyoxal2.Characterization contain 3-nitrotyrine protein in human urine by 2D-PAGE and LC/NSI/MS/MS." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/55606096158017229679.
Повний текст джерела國立中正大學
化學所
95
1. The non-enzymatic conjugated addition product of glucose or aldehyde derivatives and glycation reaction of protein are the main cause of vascular complications of diabetes. If the concentration of glucose remains at high level in the body, the amount of ?dicarbonyl compounds, such as glyoxal and methylglyoxal, also increases. Glyoxal is obtained from glucose and amino acid oxidation, lipid peroxidation; methylglyoxal is mainly from glucose degradation intermediate product: G-3-P self-decompose. These reactive ?dicarbonyl compounds react with protein, lipids and amino acids to form saccharides as the final product. This study makes use of LC/NSI/MS to investigate the reaction of glyoxal and methylglyoxal with the amino acids on protein. First, we use LC/NSI/MS to confirm that glyoxal and methylglyoxal react with N?acetyl-cysteine and N?acetyl-L-lysine to form cross-linked products. Next, we use somatostain, which contains 14 amino acids and disulfide bond to react with glyoxal and methylglyoxal respectively and we have detected their addition and cross-linked products. Finally, we investigated the selectivity of glyoxal and methylglyoxal with human hemoglobin. We found that there was a single glyoxal addition on at Lys-11 of ?globin, and Lys-16, Lys-144 and Cys-93 of β-globin:, whereas methylglyoxal were found to add ?globin at Arg-31and β-globin at Lys-144 and Arg-104. We also confirm that glyoxal forms hydroimidazolone with ?globin at Arg-92. Similar hydroimidazolone also occurs with methylglyoxal and ?globin at Arg-31and Arg-92. However, the cross-linked products of glyoxal or methylglyoxal with human hemoglobin have not been identified. 2. In cancer research, inflammation is a very crucial and dangerous factor. During infection and inflammation, the activated macrophages and neutrophils will produce excess superoxide anion and NO which will react rapidly to produce peroxynitrite. Peroxynitrite could lead to breakage and mutation of DNA, and it also reacts with protein to form 3-nitrotyrosine. Many inflammation and neural degradation diseases are related to 3-nitrotyrosine, including eye inflammation, retinal ischemia, and cancer. In 2000, a report showed that using anti-3-nitrotyrosine together with Western blotting and mass spectromety effectively identified proteins containing 3-nitrotyrosine in mouse’s retinal. This study tried to determine proteins that contain 3-nitrotyrosine in human urine using 2D-PAGE and mass spectrometry. First, we eliminated most salts in our urine sample using acetone precipitation and centrifugal device, then separated the proteins by 2D-PAGE. Next, we used anti-3-nitrotyrosine antibody-base Western blotting to locate the nitrated protein. Before identifying the proteins in 2D-PAGE, the gel was digested. Hence, different gel microwave digestion conditions were carried out to find the best conditions. The urine samples were then analyzed using this condition. Currently, we successfully identified 3 kinds proteins containing 3-nitrotyrosine, including, protein complex 4, epsilon 1 subunit; adaptor related; LIM domain only 6[Homo sapiens] and Hypothetical protein FLJ32940 isoform 1. We also found a protein, transmembrane protein 16 F[Homo sapiens], containing nitrotryptothan.
吳秀珠. "Characterization of Cu, Zn, Cd, Co-containing Biomolecules in Rabbit Serum and Supplement by 2D SEC/RPLC Chromatographic Techniques coupled with ICP-MS and ESI-MS Detection." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/11640354438277429767.
Повний текст джерелаErickson, Alison Russell. "Characterization of the Human Host Gut Microbiome with an Integrated Genomics / Proteomics Approach." 2011. http://trace.tennessee.edu/utk_graddiss/1180.
Повний текст джерелаBhandari, Devyani. "Application of solid phase extraction for storage and stability of drugs in biological fluids using 2D LC-MS technology." Thesis, 2019. https://hdl.handle.net/2144/38621.
Повний текст джерелаDjukic, Michael. "Proteomic investigations and biomarker discovery in transient ischaemic attack." Thesis, 2017. http://hdl.handle.net/2440/112817.
Повний текст джерелаThesis (Ph.D.) (Research by Publication) -- University of Adelaide, Adelaide Medical School, 2017.
Ghitun, Mihaela. "Module microfluidique intégrant des séparations multidimensionnelles : applications d'analyses protéomiques sur des extraits cellulaires." Thèse, 2006. http://hdl.handle.net/1866/17982.
Повний текст джерелаSeibert, C., B. R. Davidson, B. J. Fuller, Laurence H. Patterson, W. J. Griffiths, and Y. Wang. "Multiple-approaches to the identification and quantification of cytochromes P450 in human liver tissue by mass spectrometry." 2009. http://hdl.handle.net/10454/6179.
Повний текст джерелаKing, Siang Goh, and 吳欽翔. "Comparison and Identification of venomous components between Eastern and Western Taiwan Cobra (Naja atra) by Two Dimensional High Performance Liquid Chromatography (2D-HPLC) and Electrospray Ionization Mass Spectrometry (ESI-MS)." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/22065247906034960561.
Повний текст джерела國立清華大學
生命科學系
91
Eastern and western Taiwan Cobra (Naja atra) have been shown differences in morphology and toxicity, but the venomous components between them still remain obscure. Here we used two dimensional (Cation exchange and Reverse pharse) high performance liquid chromatography (2D-HPLC) and electrospray ionization mass spectrometry (ESI-MS) to identify and compare the venomous components between eastern and western Taiwan cobra. Molecular weight of 40 venomous proteins were determined, 12 of them were identified. A new cysteine-rich secretory protein (CRISP) with 24940 M.W. was first time found in this study. The N-terminal sequencing of this protein showed that it is highly homologous to ophanin, a CRISP isolated from king Cobra (Ophiophagus hannah) venom. Amount of this protein in crude venom is about 4-5% and showed constant expression in all Taiwan cobra. The proportions of cardiotoxin homologues were shown significantly different between eastern and western Taiwan cobra venoms. Analysis of eastern and western individual Taiwan cobra venomous components by high performance cation exchange liquid chromatography revealed cardiotoxin homologues A2 and A4 but not A6 are shown unique rich in western Taiwan cobra, the opposite result are observed in eastern Taiwan cobra. These results indicate that cardiotoxin homologues A2, A4 and A6 could become the key markers to distinguish eastern and western Taiwan cobra. In this study, we also found the northern Taiwan cobra whose morphology similar to eastern Taiwan cobra expressed both eastern and western Taiwan cobra cardiotoxin key markers. These results indicate the expression of unique cardiotoxin homologues in different geographical location might due to ecological causes.
Gabuza, Kwazikwakhe. "Identification of differentially expressed proteins in obese rats fed different high fat diets using proteomics and bioinformatics approaches." 2013. http://hdl.handle.net/11394/3954.
Повний текст джерелаObesity is a medical condition in which an energy imbalance leads to excessive accumulation of body fat. Obesity leads to a reduction in life expectancy through its association with chronic diseases of lifestyle. The prevalence of obesity is rapidly increasing throughout the world. It is now accepted that most cases of obesity result from an interaction between genetic and environmental factors. This rapid increase in obesity generally leads to an increase in morbidity and mortality from chronic diseases such as cardiovascular disease, type 2 diabetes, osteoarthritis and cancer of which obesity is a risk factor. There is a lack of information in molecular research to explain how obesity predisposes individuals to these diseases. Proteomics is a molecular tool and a set of techniques used to identify changes at protein level from a diseased state. This study aims to identify differentially expressed proteins in serum of obese rats fed different isocaloric diets using proteomics.