Готові списки джерел за темами / 1-ethyl-3-(dimethylaminopropyl)-carbodiimide

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Добірка наукової літератури з теми "1-ethyl-3-(dimethylaminopropyl)-carbodiimide"

Автор: Grafiati

Опубліковано: 11 березня 2023

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Статті в журналах з теми "1-ethyl-3-(dimethylaminopropyl)-carbodiimide"

Goyal, Navneet. "1-Ethyl-3-(3-dimethylaminopropyl) Carbodiimide Hydrochloride (EDCI×HCl)." Synlett 2010, no. 02 (January 2010): 335–36. http://dx.doi.org/10.1055/s-0029-1219043.

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NAKAZONO, Manabu, Junko IJICHI, Yoshihito OHBA, and Kiyoshi ZAITSU. "Chemiluminescence Enhancer of Polyphenols, 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide." Analytical Sciences 14, no. 4 (1998): 853–54. http://dx.doi.org/10.2116/analsci.14.853.

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Kumar, Rakesh, Awdhesh Kumar Shukla, Ellis Bagga, Sunita Kumari, Ram Prakash Bajpai, and Lalit M. Bharadwaj. "1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide interference with Lowry method." Analytical Biochemistry 336, no. 1 (January 2005): 132–34. http://dx.doi.org/10.1016/j.ab.2004.09.038.

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Wang, Qian, Hang Zhou, Yongqiang Sun, Chengbo Cao, and Kunpeng Pang. "Modified acellular porcine corneal matrix in deep lamellar transplantation of rabbit cornea." Journal of Biomaterials Applications 34, no. 8 (January 2, 2020): 1092–104. http://dx.doi.org/10.1177/0885328219898372.

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This study presents to develop a modified acellular porcine corneal matrix (MAPCM) to maintain high transparency, stability and biocompatibility as a rabbit deep cornea replacement using 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide crosslinking and a mild decellularization technique. Scaffolds are translucent and remain higher amount of glycosaminoglycans after decellularization than acellular porcine corneal matrix (APCM). Enzymatic degradation kinetics and mechanical properties of scaffolds are regulated by 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide -crosslinking density. The porous structure and ultrastructure of collagenous lamellae are maintained, and the pore size of MAPCM crosslinked with 0.5% (w/v) 1-ethyl-3–(3-dimethylaminopropyl)-carbodiimide is 13.26 ± 1.65 µm, similar to that of normal porcine cornea. The transmittance of MAPCM gets 79.1 ± 0.45 to 92.7 ± 1.4% in the visible light range. Results from a CCK-8 assay indicate that MAPCM gets higher cell proliferation rate of rabbit corneal stroma cells than APCM. Since collagen fibres structural integrity and regularity of MAPCM are retained after crosslinking, the opacity and stability of MAPCM are better than those of APCM within 4 weeks of animal implantation. In addition, there is no indication of an immune response or neovascularization in or around the transplanted disc. These results reveal that MAPCM may be a more suitable scaffold for corneal substitute construction.

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Miao, Yanming. "Application of BSA-bioconjugated phosphorescence nanohybrids in protein detection in biofluids." RSC Advances 5, no. 94 (2015): 76804–12. http://dx.doi.org/10.1039/c5ra11691k.

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In this study, a cross-linking agent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) was used to link QDs and bovine serum albumin (BSA) to form a nanohybrid BSA–Mn-ZnS Room-Temperature Phosphorescence (RTP) biosensor.

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Lee, J. M., H. H. L. Edwards, C. A. Pereira, and S. I. Samii. "Crosslinking of tissue-derived biomaterials in 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)." Journal of Materials Science: Materials in Medicine 7, no. 9 (September 1996): 531–41. http://dx.doi.org/10.1007/bf00122176.

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Kubilius, Matthew B., and Raymond S. Tu. "Circular Dichroistic Impacts of 1-(3-Dimethylaminopropyl)-3-ethylurea: Secondary Structure Artifacts Arising from Bioconjugation Using 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide." ACS Omega 2, no. 11 (November 21, 2017): 8308–12. http://dx.doi.org/10.1021/acsomega.7b01288.

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Wang, Delong, та Hui Shi. "An Unexpected Reaction of Isodehydracetic Acid with Amines in the Presence of 1-Ethyl-3-(3-dimethylaminopropyl) Carbodiimide Hydrochloride Yields a New Type of β-Enaminones". Molecules 25, № 9 (2 травня 2020): 2131. http://dx.doi.org/10.3390/molecules25092131.

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Анотація:

The reaction of isodehydracetic acid with amines was serendipitously found to afford β-enaminones in the presence of the coupling agent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC). Under the optimal reaction condition, 23 examples of α-aminomethylene glutaconic anhydride were obtained at approximately 30−80% yields. This is a concise, operationally simple method to expediently synthesize a new type of β-enaminone-containing compound.

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Tropini, V., J. P. Lens, W. J. Mulder, and F. Silvestre. "Wheat gluten films cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide." Industrial Crops and Products 20, no. 3 (November 2004): 281–89. http://dx.doi.org/10.1016/j.indcrop.2003.10.012.

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Stankova, Ivanka, Stoyan Schichkov, Kalina Kostova, and Angel Galabov. "New Analogues of Acyclovir – Synthesis and Biological Activity." Zeitschrift für Naturforschung C 65, no. 1-2 (February 1, 2010): 29–33. http://dx.doi.org/10.1515/znc-2010-1-205.

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New acyclovir esters with peptidomimetics were synthesized and evaluated in vitro for their antiviral activity against the replication of Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The infl uence of peptidomimetics containing oxazole and thiazolyl-thiazole moieties on the antiviral activity is also reported. The esters were synthesized using the coupling reagents N-ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N,N-dimethyl-4-aminopyridine (DMAP) as a catalyst.

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Більше джерел

Дисертації з теми "1-ethyl-3-(dimethylaminopropyl)-carbodiimide"

Lampinen, Salomonsson Matilda. "Chemical Derivatization in Combination with Liquid Chromatography Tandem Mass Spectrometry for Detection and Structural Investigation of Glucuronides." Doctoral thesis, Uppsala University, Analytical Pharmaceutical Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8670.

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This thesis presents novel approaches for structural investigation of glucuronides using chemical derivatization in combination with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MSⁿ).

Today, LC-ESI-MSⁿ is the dominant technique for quantitative as well as qualitative analyses of metabolites, due to its high sensitivity and selectivity. However, for compounds without an easily ionizable group, e.g., steroids, the sensitivity is limited. In the work presented in this thesis, a derivatization procedure forming a basic oxime significantly increased the detection sensitivity for the altrenogest glucuronide.

Furthermore, in structural evaluations of glucuronides, the limitation of LC-MSⁿ becomes evident due to the initial neutral loss of 176 u, i.e. monodehydrated glucuronic acid, which often makes it impossible to elucidate the structures of the conjugates. To solve this problem, the main part of the work described in this thesis was devoted to chemical derivatization as a means of facilitating the determination of the site of conjugation.

For the first time, the isomeric estriol glucuronides were evaluated using a combination of three reagents 2-chloro-1-methylpyridinium iodide (CMPI), 1-ethyl-3-(3-dimethyl- aminopropyl)-carbodiimide (EDC), and 2-picolylamine (PA). Interestingly, the derivatization gave a selective fragmentation pattern leading to differentiation of the isomers.

Another derivatization reagent, 1,2-dimethylimidazole-4-sulfonyl chloride (DMISC), was also tested for the first time in structural investigations. The isomeric glucuronides of morphine, formoterol, and hydroxypropranolol were evaluated. They can all be conjugated in aliphatic as well as aromatic positions. DMISC was proven to be useful in two ways. Firstly, the morphine and formoterol glucuronides that contained a free phenol could be differentiated from those that were conjugated in the aromatic position based on different reactivity. Secondly, for the aromatic O-glucuronide of 4’-hydroxypropranolol, DMISC was proven to react with the amine. This product gave a different fragmentation pattern compared to the corresponding derivative of the aliphatic glucuronide.

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VOTTARIELLO, FRANCESCA. "OLIGOMERIZATION OF RNase A:a) A STUDY OF THE INFLUENCE OF SERINE 80 RESIDUE ON THE 3D DOMAIN SWAPPING MECHANISMb) “ZERO-LENGTH” DIMERS OF RNase A AND THEIR CATIONIZATION WITH PEI." Doctoral thesis, 2010. http://hdl.handle.net/11562/344075.

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"Zero-length" dimers of ribonuclease A, a novel type of dimers formed by two RNase A molecules bound to each other through a zero-length amide bond [Simons, B.L. et al. (2007) Proteins 66, 183-195], were analyzed, and tested for their possible in vitro cytotoxic activity. Results: (i) Besides dimers, also trimers and higher oligomers can be identified among the products of the covalently linking reaction. (ii) The "zero-length" dimers prepared by us appear not to be a unique species, as was instead reported by Simons et al. The product is heterogeneous, as shown by the involvement in the amide bond of amino and carboxyl groups others than only those belonging to Lys66 and Glu9. This is demonstrated by results obtained with two RNase A mutants, E9A and K66A. (iii) The "zero-length" dimers degrade poly(A).poly(U) (dsRNA) and yeast RNA (ssRNA): while the activity against poly(A).poly(U) increases with the increase of the oligomer's basicity, the activity towards yeast RNA decreases with the increase of oligomers' basicity, in agreement with many previous data, but in contrast with the results reported by Simons et al. (iv) No cytotoxicity against various tumor cells lines could be evidenced in RNase A "zero-length" dimers. (v) They instead become cytotoxic if cationized by conjugation with polyethylenimine [Futami, J. et al. (2005) J. Biosci. Bioengin. 99, 95-103]. However, polyethylenimine derivatives of RNase A "zero-length" dimers and native, monomeric RNase A are equally cytotoxic. In other words, protein "dimericity" does not play any role in this case. Moreover, (vi) cytotoxicity seems not to be specific for tumor cells: polyethylenimine-cationized native RNase A is also cytotoxic towards human monocytes.
"Zero-length" dimers of ribonuclease A, a novel type of dimers formed by two RNase A molecules bound to each other through a zero-length amide bond [Simons, B.L. et al. (2007) Proteins 66, 183-195], were analyzed, and tested for their possible in vitro cytotoxic activity. Results: (i) Besides dimers, also trimers and higher oligomers can be identified among the products of the covalently linking reaction. (ii) The "zero-length" dimers prepared by us appear not to be a unique species, as was instead reported by Simons et al. The product is heterogeneous, as shown by the involvement in the amide bond of amino and carboxyl groups others than only those belonging to Lys66 and Glu9. This is demonstrated by results obtained with two RNase A mutants, E9A and K66A. (iii) The "zero-length" dimers degrade poly(A).poly(U) (dsRNA) and yeast RNA (ssRNA): while the activity against poly(A).poly(U) increases with the increase of the oligomer's basicity, the activity towards yeast RNA decreases with the increase of oligomers' basicity, in agreement with many previous data, but in contrast with the results reported by Simons et al. (iv) No cytotoxicity against various tumor cells lines could be evidenced in RNase A "zero-length" dimers. (v) They instead become cytotoxic if cationized by conjugation with polyethylenimine [Futami, J. et al. (2005) J. Biosci. Bioengin. 99, 95-103]. However, polyethylenimine derivatives of RNase A "zero-length" dimers and native, monomeric RNase A are equally cytotoxic. In other words, protein "dimericity" does not play any role in this case. Moreover, (vi) cytotoxicity seems not to be specific for tumor cells: polyethylenimine-cationized native RNase A is also cytotoxic towards human monocytes.

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Частини книг з теми "1-ethyl-3-(dimethylaminopropyl)-carbodiimide"

Edwin, Boby T., H. Dhanya, Prabha D. Nair, and Moustapha Kassem. "In Vitro and In Vivo Evaluation of 1-(3 Dimethylaminopropyl)-3-Ethyl Carbodiimide (EDC) Cross-Linked Gum Arabic–Gelatin Composite as an Ideal Porous Scaffold for Tissue Engineering." In Biomaterials in Orthopaedics and Bone Regeneration, 131–45. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-9977-0_9.

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Тези доповідей конференцій з теми "1-ethyl-3-(dimethylaminopropyl)-carbodiimide"

Wang, Wei-Jhen, Chia-Hwa Lee, Chin-Wen Li, Stephen Liao, Fuh-Jyh Jan, and Gou-Jen Wang. "Direct Label Free Detection of Orchid Virus Using a Micro/Nano Hybrid Structured Biosensor." In ASME 2019 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2019. http://dx.doi.org/10.1115/detc2019-97198.

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Abstract In this study, a label-free detection approach for effective detection of the odontoglossum ringspot virus (ORSV) infected orchids has been developed. We used semiconductor fabrication process to fabricate 1,810 micro/nano hybrid structured sensing electrodes on a 8 inch reclaimed wafer. The self-assembled monolayer (SAM) process was then employed to sequentially modify the electrode surface with 11-mercaptoundecanoic acid (11-MUA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/ N-hydroxysuccinimide (NHS), anti-ORSV, and ORSV. EIS was conducted for the ORSV concentration detection. Experimental results demonstrated that the ORSV concentration in a virus infected orchid leaf could be effectively detected. When compared with the ELISA kit, our device possesses a wider linear detection range (0.5–50,000 ng/mL) and a higher sensitivity. The specificity of our device on ORSV detection was also confirmed. Our sensing device retains advantages, such as label-free, lower amounts of the antibody and target sample required, low detection time, and a wider linear detection range. Those results imply the feasibility of our sensing device in field applications.

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Gee, Albert O., Brendon M. Baker, and Robert L. Mauck. "Mechanics and Cytocompatibility of Genipin Crosslinked Type I Collagen Nanofibrous Scaffolds." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-193220.

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Collagen is a principal constituent of the extracellular matrix (ECM) and as such, defines the microenvironmental milieu in which cells reside. In fiber-reinforced musculoskeletal tissues, collagen fibers are highly organized and generate the direction-dependent mechanical properties critical to the function of these structures. Given its primary role, collagen is particularly attractive for tissue engineering (TE) applications where scaffolds are coupled with cells to repair or regenerate damaged tissues. One method for producing collagen-based scaffolds is through electrospinning. This technique yields nano- to micron-scale fibers similar in diameter to those of the native ECM. Towards engineering orthopaedic tissues, methods have been devised to electrospin fibers into aligned arrays that can recapitulate the anisotropy of fiber-reinforced tissues [1]. While a number of polymers have been electrospun, collagen-based scaffolds are especially promising as they provide a biomimetic interface for cell attachment [2]. Numerous investigators have electrospun collagen [3], one major drawback is their inherent instability in aqueous environments. To address this, various crosslinking agents including glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride, and N-hydroxysuccinimide chemistries have been used, but these chemicals often prove cytotoxic or excessively laborious in application [4]. Even with crosslinking, dry as-formed nanofibrous collagen scaffolds with moduli greater than 50MPa diminish by 100-fold with rehydration [5].

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