Добірка наукової літератури з теми "004.05 (0.43.3)"

In order to establish the biochemical basis for dietary interventions, we investigated the fatty acid composition of plasma lipid classes in patients with inactive inflammatory bowel disease. In this cross-sectional study thirty patients with ulcerative colitis (UC), twenty-one with Crohn disease (CD) and twenty-four controls were investigated (mean age: UC, 40·8 (sd12·1); CD, 37·6 (sd11·0); control, 31·5 (sd8·4) years). Fatty acid composition of plasma lipids was determined by high-resolution capillary GLC. In plasma phospholipids, significantly higher values of eicosapentaenoic (20 : 5n-3), docosapentaenoic (22 : 5n-3) and γ-linolenic (18 : 3n-6) acids were found in control patients and patients with UC as compared to patients with CD [median % (weight by weight), controlv.UCv.CD : 20 : 5n-3, 0·09 (interquartile range (IQR) 0·05)v.0·14 (IQR 0·10)v.0·16 (IQR 0·10),P < 0·05; 22 : 5n-3, 0·14 (IQR 0·10)v.0·27 (IQR 0·16)v.0·31 (IQR 0·10),P < 0·001; 18 : 3n-6, 0·02 (IQR 0·02)v.0·03 (IQR 0·02)v.0·05 (IQR 0·03),P < 0·05]. When compared to the control, values of the principaln-3 andn-6 long-chain PUFA, arachidonic acid (20 : 4n-6) and DHA (22 : 6n-3) were significantly higher in patients with UC but not in patients with CD [median % (w/w), UCv.control: 20 : 4n-6, 8·43 (IQR 3·23)v.6·92 (IQR 2·96),P < 0·05; 22 : 6n-3, 1·22 (IQR 0·56)v.0·73 (IQR 0·39),P < 0·05]. As seen there are considerable differences between the long-chain PUFA status of patients suffering from UC or CD. The data obtained in the present study do not support the concept of eicosapentaenoic acid or DHA deficiency in patients with either UC or CD.

The objective of the present study was to investigate the relationship between plasma n-3 PUFA composition and weight status. A total of 124 adults, stratified by weight status: healthy weight (n 21), overweight (n 40) and obese (n 63) were recruited. Fasting blood samples, anthropometric measures and body composition were collected. Plasma fatty acid composition was determined by GC. BMI, waist circumference and hip circumference were inversely correlated with n-3 PUFA, EPA and DHA (P < 0·05 for all) in the obese group. Obese individuals had significantly lower plasma concentrations of total n-3 PUFA, compared with healthy-weight individuals (4·53 (sd 1·11) v. 5·25 (sd 1·43) %). When subjects were pooled and stratified into quartiles of total n-3 PUFA, a significant inverse trend was found for BMI (P = 0·002), waist circumference and hip circumference (P = 0·01 and P < 0·001 respectively). Higher plasma levels of total n-3 PUFA are associated with a healthier BMI, waist circumference and hip circumference. Our findings suggest that n-3 PUFA may play an important role in weight status and abdominal adiposity.

SUMMARYIn Australia, hepatitis B (HBV) vaccination is recommended for injecting drug users (IDUs), Indigenous adults and prisoners. We compared immunity to HBV in prisoners and the general population obtained from national serosurveys in 2007. Individuals with HBV surface antibody (HBsAb) positive sera were considered immune from past infection [HBV core antibody (HBcAb) positive] or from vaccination (HBcAb negative). Male prisoners aged 18–58 years had a higher HBsAb seroprevalence than the general population (46·4% vs. 39·4%, P = 0·061). Comparison of HBcAb results was possible for males aged 18–29 years. In this group, higher HBsAb seroprevalence was due to past infection (12·9% vs. 3·0%, P < 0·001), rather than vaccine-conferred immunity (35·3% vs. 43·4%, P = 0·097). All prisoner groups, but especially IDUs, those of Indigenous heritage or those with a previous episode of imprisonment had higher levels of immunity from past infection than the general population (19·3%, 33·0%, 17·1%, respectively, vs. 3·0%, P < 0·05). Indigenous prisoners, non-IDUs and first-time entrants had significantly lower levels of vaccine-conferred immunity than the general population (26·4%, 26·2% and 20·7% respectively vs. 43·4%, P < 0·05). Improving prison-based HBV vaccination would prevent transmission in the prison setting and protect vulnerable members of the community who are at high risk of both infection and entering the prison system.

AbstractLitter size of commercial European sow lines can be increased by the introduction of genes from the highly prolific, but obese Meishan. The introduction of these genes into sow lines may necessitate different nutritional strategies for lactation. A 2 × 2 × 2 factorial experiment was used to investigate the influence of breed type (Meishan synthetic 50% (M) or purebred European Landrace and Large White (W)) and parity (1 or 3) on response to dietery protein level (H = 178 g crude protein (CP) per kg 8 g lysine per kg, L = 136 g CP per kg 5 g lysine per kg over a 5-week lactation. M were lighter and fatter at farrowing than W (P < 0·001), consumed more food (M = 7·33 v.W = 6·98 (s.e.d. 0·17) kg/day, P < 0·05) but had higher maternal weight loss (M = 16·2 v.W = 11·9 (s.e.d. 1·88) kg, P < 0·05) and higher fat loss (M = 6·5 v. W = 26 (s.e.d. 077) mm P2, P < 0·001) during lactation. Litter growth rate was higher for M than for W (P < 0·005) and for H than for L (P < 0·005). Differences were found between breeds in the partitioning of food protein. Overall, higher correlations between lysine intake and litter growth rates were found in M (r = 0·55, P < 0·001) than in W (r = 0·13, P > 0·05). Lysine intake and sow weight loss were more correlated in W(r = 0·66, P < 0·001) than in M (r = 0·15), P > 0·05). Fat loss was correlated with initial fat depth in M (r = 0·61, P < 0·001) but not in W. Weaning to conception intervals showed a breed × diet interaction (loge days: ML = 1·38, MH = 1·55, WL = 1·73, WH = 1·43, s.e.d. 0·14, P < 0·05). It is concluded that M, with greater initial fat reserves, catabolize more body fat to support greater milk output. This is facilitated by provision of a high protein diet. The W sows do not utilize the extra protein for milk production but instead use it to minimize weight loss (maintain protein status of their own body) which may help prevent an extended weaning to conception interval.

AbstractObjectiveTo describe dietary changes in men participating in an obesity intervention as part of the Self-Help, Exercise and Diet using Information Technology (SHED-IT) study.DesignAn assessor-blinded randomized controlled trial comparing Internet (n 34) v. information-only groups (n 31) with 6-month follow-up. Dietary intake assessed by FFQ, reporting usual consumption of seventy-four foods and six alcoholic beverages using a 10-point frequency scale. A single portion size (PSF) factor was calculated based on photographs to indicate usual serving sizes.SettingThe campus community of the University of Newcastle, New South Wales, Australia.SubjectsSixty-five overweight/obese men (43 % students, 42 % non-academic general staff, 15 % academic staff; mean age 35·9 (sd 11·1) years, mean BMI 30·6 (sd 2·8) kg/m2).ResultsThe average PSF decreased significantly over time (χ2 = 20·9, df = 5, P < 0·001) with no differences between groups. While both groups reduced mean daily energy intake (GLM χ2 = 34·5, df = 3, P < 0·001), there was a trend towards a greater reduction in the Internet group (GLM χ2 = 3·3, P = 0·07). Both groups reduced percentage of energy from fat (P < 0·05), saturated fat (P < 0·001) and energy-dense/nutrient-poor items (P < 0·05), with no change in dietary fibre or alcohol (P > 0·05).ConclusionsAlthough men reported some positive dietary changes during weight loss, they did not increase vegetable intakes nor decrease alcohol consumption, while saturated fat, fibre and Na intakes still exceeded national targets. Future interventions for men should promote specific food-based guidelines to target improvements in their diet-related risk factor profile for chronic diseases.

AbstractCurrent feeding recommendations for lactating sows may be inappropriate for modern genotypes with high milk production. The introduction of the prolific but obese Meishan breed into sow lines also necessitates a review of nutrient requirements. A 2 × 2 factorial experiment was used to investigate the influence of breed type (Meishan synthetic 50% (M) and purebred European White (W) on response to dietary protein level (L = 140 g crude protein (CP) per kg, 6 g lysine per kg; H = 181 g CP per kg, 9 g lysine per kg) over a 5-week lactation. Milk yield (days 5, 9, 13,17, 21 and 28), milk composition and piglet growth and body composition were measured to quantify aspects of lactational performance. M had higher litter sizes throughout lactation (P < 0·001) and produced more milk than W(M = 10·04 W = 7·85 (s.e.d. 0·521) kg/day, P < 0·001) and milk of a higher fat content (M = 79·6 W = 75·0 (s.e.d. 5·71 g/kg, P < 0·05). Total solids were not significantly different between treatments. H produced higher milk protein content than L (L = 43·0 H = 55·7 (s.e.d. 3.01) g/kg, P = 0·05). Litter growth rates showed a breed effect (P < 0·001), diet effect (P < 0·001) and a breed × diet interaction (ML = 2·41 MH = 3·21 WL = 2·07 WH = 2·07 (s.e.d. 0·658) kg/day, P < 0·001). M piglets had higher fat deposition rates (M = 37 W = 24 (s.e.d. 4.5) g/day, P < 0·05). L piglets had a lower protein (P < 0·05) and fat (P < 0·05) deposition rate compared with H piglets. Higher litter growth rates of M sows resulted from a combination of increased milk yield and f at content and this was further enhanced by additional dietary protein. W sows showed no milk yield response to dietary protein but reduced maternal tissue catabolism.

AbstractThe objective was to determine the daily live-weight gain, behaviour, adrenal and immune responses of finishing beef heifers housed at two different space allowances. Heifers (no. = 32) with a mean live weight of466 (s.e. 3·6) kg were assigned to either 1·5 or 3·0 m2 average individual space allowance in four slatted-floor pens (two per treatment) for a period of 104 days. On days 5, 40, 68 and 96, heifers (no. = eight per treatment, four per pen) were challenged with 1·98 i.u. ACTH per kg M0·75, and serial blood samples were analysed for plasma cortisol concentrations. The other 16 heifers were immunized against keyhole limpet haemocyanin (KLH) on day 28, and blood samples collected on days 28, 42, 56, 70, 84 and 98 were analysed for anti-KLH immunoglobulin (Ig) concentrations. All heifers were blood sampled on days 0, 14, 56 and 98, and red and white blood cell numbers, packed cell volume (PCV) and plasma concentrations of creatine kinase (CK), non-esterified fatty acids (NEFA) and urea were determined. Behavioural observations were conducted on days 8, 43, 71 and 99 by scan sampling for 24 h (10-min intervals), and continuous 4-h observations were conducted on days 100 and 101.Heifers at 1·5 m2 space allowance had a lower daily live-weight gain compared with those at 3·0 m2 (0·60 v. 0·87 (s.e. 0·04) kg; P < 0·001). Heifers at 1·5 m2 had lower pre-ACTH baseline cortisol concentrations (P < 0·05) and lower post-ACTH peak cortisol concentrations (P < 0·05). There were no effects of treatment on serum anti-KLH IgG1 or IgG2 responses (P > 0·05). Heifers at 1·5 m2 had lower plasma NEFA concentrations (P < 0·05). Red and white blood cell numbers, PCV, CK and urea were not affected by treatment (P > 0·05). The time spent lying down was lower for heifers at 1·5 m2 (10·0 v. 21·1 h/day; P < 0·05). Social interactions were fewer (P < 0·05), and incidence of head-resting behaviour was higher (P < 0·05) among heifers at 1·5 m2 compared with 3·0 m2 space allowance. In conclusion, the restricted space allowance resulted in a substantial decrease in daily live-weight gain, and changes in adrenal response and behaviour.

AbstractThe effects of three fat supplements on milk yield and composition were measured using 12 mid-lactation in-calf Hoistein-Friesian cows in a balanced incomplete change-over design over three periods each of 3 weeks. All cows received a basal diet consisting of 36 kg/day grass silage (dry matter (DM) 270 g/kg, metabolizable energy (ME) 11·6 MJ/kg DM) and 7 kg/day o f a concentrate mixture containing (g/kg) rolled barley (501), molassed sugar-beet pulp shreds (277), soya-bean meal (208) and a standard cow mineral supplement (14). Treatments were CON (control-no supplement); LIN and FISH (250 gl day of either linseed oil or marine oil, providing approximately 0·046 of ME intake) or TOA (95 glday of tuna orbital oil, providing 0·018 of total ME intake).There were no significant effects on silage DM intake or milk yield (means 9·25 and 17·2 kg/day respectively). The FISH and TOA treatments depressed (F < 0·05) milk fat concentration (45·4, 44·6, 34·5 and 41·6 (s.e.d. 1·08) g/kg for CON, LIN, FISH and TOA respectively; note — the same treatment order is used for all results quoted). Compared with values for CON, yield of f at (glday) was significantly (F < 0·05) greater for LIN and significantly lower for FISH (739, 808, 572 and 732, s.e.d. 28·7). All three oil supplements reduced (F < 0·05) milk protein content (33·6, 32·5, 30·6 and 32·4 (s.e.d. 0·43) g/kg) but, apart from a small increase for LIN, protein yield (glday) was unaffected (545, 586, 510 and 574, s.e.d. 20·2).The concentrations (g/100 g) of short-chain fatty acids (< C14) and C16 : 0 in milk f at were lower (F < 0·05) for LIN than for the other treatments. All supplements increased the concentrations ofC18:1 (F < 0·05), the value for LIN being greater (F < 0·05) than for the other treatments (21·0, 27·2, 25·3 and 23·7, s.e.d. 0·74). The FISH and TOA treatments increased (F < 0·05) the concentrations of long chain (< C2O) (n-3) poly-unsaturated fatty acids (PUFA), (0·19, 0·17, 0·49 and 0·27, s.e.d. 0·026) but less than proportionately 0·03 of dietary intake of these acids was transferred to milk, probably because they were found to be mostly in the phospholipid and cholesterol ester fractions of plasma. The FISH and TOA treatments increased (F < 0·05) the percentages of total trans fatty acids in milk fat (1·13, 2·19, 10·26 and 3·62, s.e.d. 0·728) whilst a significant (F < 0·05) increase in conjugated linoleic acid (CLA) was observed only for FISH (0·16, 0·28, 1·55, and 0·52, s.e.d. 0·154). Concentrations of CLA and total trans acids in milk were highly correlated (r = 0·91, no. =36, F < 0·001) whilst trans acids in milk were inversely correlated with milk fat content (r = -0·63, no. = 36, F < 0·001) supporting the theory that milk fat depression may be caused by increased supply of trans fatty acids to the mammary gland. The health implications of these changes in milk fat composition are discussed.

SUMMARYIn order to study the epidemiology of acute otitis media (AOM) and Streptococcus pneumoniae nasopharyngeal colonization in the first 2 years of life, we followed up an unvaccinated birth cohort monthly and at visits when sick, with otoscopy to detect AOM and performed nasopharyngeal swabbing to detect S. pneumoniae. Serotyping of positive cultures was also performed. Of 210 babies who were enrolled at birth, 61 (29·05%) experienced 128 episodes of AOM [relative risk 2·63, 95% confidence interval (CI) 1·21–5·75] with maximum incidence in the second half of the first year of life. Episodes ranged from 1 to 7 (mean 2·1 episodes). Most (86·9%) babies with AOM had a positive culture swab giving an odds ratio (OR) of 1·93 (95% CI 1·03–3·62, P = 0·041) for this association. Other risk factors identified for AOM were winter season (OR 3·46, 95% CI 1·56–7·30, P = 0·001), upper respiratory infection (OR 2·43, 95% CI 1·43–4·51, P = 0·005); residents of small households were less likely to develop AOM (OR 0·32, 95% CI 0·17–0·57, P < 0·01). Common S. pneumoniae serotypes isolated during episodes were 19, 6, 15, 35, 7, 23, 9 and 10 which indicated a theoretical coverage for pneumococcal vaccines PCV10 and PCV13 constituent serotypes of 62·8%. We conclude that AOM in Indian infants is often associated with S. pneumoniae colonization of the nasopharynx as well as other risk factors.

ABSTRACTA study was made of the effect of fibre level in forages on eating and rumination behaviour. Eight forage diets were prepared from the leaf and stem fractions of two grasses and two legumes and were given at hourly intervals to four steers under steady-state conditions. Eating and rumination behaviour were measured automatically by recording changes in intra-oesophageal pressure.Mean voluntary intake of leaf was higher than that of the stem fractions (9·9 v. 5·6 kg/day; P < 0·001). This was associated with a shorter mean retention time in the rumen of the leaf than that of the stem fractions (21·4 v. 30·6 h; P < 0·001) and a lower concentration (g/100 g dry matter (DM)) of fibre (52-0 neutral-detergent fibre (NDF) and 30·5 acid-detergent fibre (ADF) v. 68·2 NDF and 45·3 ADF). Similar values (P > 0·05) between diets were found for both the water and DM contents of the rumen (60·1 kg, 7·8 kg). Voluntary intake was not related to either.No difference was found between forage diets in the mean time (132 min) and number (18·7) of periods spent eating each day (P > 0·05). Legume leaf fractions were eaten at a faster rate (g/min) than either the grass leaf or the stem fractions. Voluntary intake was related to the rate at which food was eaten (r = 0·89; P < 0·01) but no relationship was found with the time taken to eat food (r = –0·14; P>0·05). Eating rate was related to the level of both NDF (r = –0·91; P < 0·01) and ADF (r = –0·96; P < 0·001).Differences between diets were found in rumination times (mean 425 min; P < 001), the number of boluses regurgitated during each period (27·6; P < 0·05) and during each day (485; P < 0·001), and in the weight of boluses (455 g; P < 0·05). No differences (P > 0·05) were found between diets in the mean number of rumination periods each day (17·6), the mean time spent ruminating during each period (24·3 min), the mean rate at which boluses were regurgitated (53·2 s per bolus), the interval between boluses (5·1 s), and the DM in a bolus (27·5 g). Rumination time and the number of boluses regurgitated either per period or per day were not related to the fibre content of the diet (P > 0·05).The regurgitated boluses from leaf fractions were chewed less than the stem fractions (43·7 v. 54·7 chews per bolus). The regurgitated boluses of lucerne leaf were chewed at a faster rate (1·13 chews per s; P > 0·05) than regurgitated digesta of the other diets which were chewed at similar rates (0·97 chews per s; P > 0·05). The total number of rumination chews made each day by animals given lucerne leaf (12 300) was much lower (P < 0·001) than that by animals given the other fractions (25 300). The number of chews made on each bolus was related to fibre levels in the diets (NDF, r = 0·78, P < 0·05; ADF, r = 0·91, P < 0·01).It is concluded that the voluntary intake of high-fibre diets is not always restricted by rumen fill or rumination. The ease with which forage is eaten should be investigated as a factor influencing intake of fibrous forages.