Дисертації з теми "Γ Amino Acid"
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Stanovych, Andrii. "Synthèse et études structurales de γ-peptides synthétisés à partir d’acides β,γ-diaminés". Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112310/document.
Повний текст джерелаThe design of a new oligopeptides, capable to mimic the properties of natural proteins, is an important field not only for structural studies but also in the developpement of new efficient drugs. The peptides featuring β-amino acids have been extensively explored, whereas the research in γ-peptides is more recent. The studies of γ-peptides show their ability to adopt stable secondary structures and also to have a promising biological activity. Our laboratory is interested in the synthesis of new β,γ-diaminoacids.The aim of this work is a developpement of new synthetic route starting from different natural α -amino acids and to use obtained β,γ-diaminoacids to access novel unnatural peptides having specific conformational properties.The synthetic strategy, developed and optimized in our laboratory during this work, gives access to diastereomers cis and trans from L-leucine, L- and D-phenylalanine which were used in the synthesis of a new hybrid α/γ-peptides. The structural studies were performed on two series of hybrid α/γ-peptides consisting of β,γ-aminoacid from L-leucine and L- or D-α-amino acids. In the first case, the peptides are able to adopt stable secondary structures stabilized by intramolecular hydrogen bonds involving the nitrogen on the β-position. In addition, we present the synthesis of an analogue of gramicidine S, a naturally occuring antibiotic cyclic peptide. The dipeptide pattern D-Phe-L-Pro has been replaced with the β,γ-diaminoacid synthesized from D-phenylalanine
Bonnel, Clément. "Oligopeptides construits autour du γ-aminoacide ATC : synthèses, analyses structurales et évaluation biologique". Thesis, Montpellier, 2016. http://www.theses.fr/2016MONTT201.
Повний текст джерелаThis manuscript describes the synthesis, the structural study and the biological evaluation of abiotic oligopeptides incorporating the heterocyclic gamma-amino acid ATC (4-Aminomethyl-1,3-Thiazole-5 Carboxylic acid). This original block is built around a thiazole ring and displays two lateral chains. Previous work in our laboratory highlighted that the presence of the thiazole ring between the positions alpha and beta implied that zeta angle was blocked around 0°, thus structuring poly-(S)-ATCs homo-oligomers in a right-handed 9-helix foldamer. First, development of a simple, flexible and enantioselective synthesis on a few grams scale has allowed to get access of a highly diverse ATC library from commercial alpha-amino acids. Introduction of the chemical diversity occurs via two key steps implying a cross-Claisen condensation and a Hantzsch cyclization. Then identification of NMR and FT-IR structural markers of ATC-containing oligomers was used to characterize the folding propensity of hybrid α:ATC oligomers. We demonstrated that 1:1 (L)-alpha:(S)-ATC heterochiral oligomers are structured in solution in a new ribbon-like shape stabilized by a bidentate intramolecular hydrogen bond network forming 9- and 12-membered pseudorings. The distribution of lateral chains along the main skeleton shows a high analogy with alpha-helix thus constituting a major advantage for potential medicinal applications. The last part of this work has focused on the design of amphipathic ATC-containing pseudo-peptides as antimicrobial agents
Wenjie, Lu. "Synthesis and Evaluation of Functionalized Dirhodium(II) Carboxylate Catalysts Bearing Axially Chiral Amino Acid Derivatives." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225528.
Повний текст джерелаWersinger, Eric. "Inhibition présynaptique et contraste dans la rétine de souris : à la recherche du rôle des dystrophines." Paris 6, 2007. http://www.theses.fr/2007PA066122.
Повний текст джерелаOlszewski, Tomasz Krzysztof. "Original syntheses of new biomolecules : from imidazole and thiazole derivatives of α-aminophosphonic acids and γ-amino-α, β-dihydroxy carboxylic acid derivatives to disaccharide mimics". Montpellier 2, 2006. http://www.theses.fr/2006MON20017.
Повний текст джерелаFlanigan, David L. Jr. "Studies in Rhodium Catalyzed Intramolecular C-H Insertion of Amino Acid Derived α-Diazo-α-(substituted)acetamides and its Application to the Total Synthesis of clasto-Lactacystin β-Lactone". Scholar Commons, 2004. https://scholarcommons.usf.edu/etd/1037.
Повний текст джерелаWan, Yang. "Synthesis of β,γ-diamino acids and their use to design new analogues of the antimicrobial peptide Gramicidin Septide antimicrobien, la Gramicidine S". Thesis, Université Paris-Saclay (ComUE), 2017. http://www.theses.fr/2017SACLS407/document.
Повний текст джерелаIn our group, we are interested in developing peptides containing β,γ-diamino acids . Along with many other peptides containing unnatural amino acids, they have shown the ability to possess stable conformations and/or interesting biological activities. Moreover, those peptides are usually more resistant to proteolysis. In order to synthesize stereopure γ-amino acids, we have developed a synthetic route using Blaise reaction and subsequent diastereoselective reduction as key reactions. Through applying this method, we have synthesized β,γ-diamino acids derived from D-phenylalanine and L-glutamic acid. The former β,γ-diamino acid was used for designing antimicrobial peptide gramicidin S analogues. Compared with mother molecule, the analogues exerted much less host cell cytotoxicity while remaining interesting antibacterial activity. Meanwhile, it gave us more knowledge for further developing analogues of gramicidin S as well as other antimicrobial peptides. We also paid lots of effort to efficiently synthesize cyclic β,γ-diamino acids starting from L-glutamic acid. Interestingly, when oligomers incorporating this β,γ-diamino acids and α-amino acids, they have shown the potential to adopt stable conformations. The following studies will be continuously investigated
Zammit, Charlotte Maria. "Asymmetric synthesis of β- and γ- amino acids". Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:35138286-c217-4d28-990b-941149b5daa5.
Повний текст джерелаHallinan, Keith Oliver. "Synthetic and metabolic studies on β,γ-unsaturated α-amino acids". Thesis, University of Warwick, 1992. http://wrap.warwick.ac.uk/110385/.
Повний текст джерелаKerres, Sabine Martha [Verfasser], та Oliver [Akademischer Betreuer] Reiser. "Synthesis of γ-cyclobutane amino acids via visible light / Sabine Martha Kerres ; Betreuer: Oliver Reiser". Regensburg : Universitätsbibliothek Regensburg, 2019. http://d-nb.info/1196873410/34.
Повний текст джерелаAwada, Hawraà. "Synthèse sélective de γ-amino acides cyclobutaniques : préparation de nouveaux organogélateurs peptidiques". Thesis, Paris 11, 2014. http://www.theses.fr/2014PA112365/document.
Повний текст джерелаThe γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system (CNS). In order to obtain new enantiomerically pure cyclobutanic derivative of GABA, the cis-3,4CB-GABA, two efficient synthetic strategies have been established. Both synthetic routes employed a photocycloaddition [2 +2] protocol, which provided the cyclobutanic ring. The first route involved the homolgation of the cis-2-aminocyclobutanecarboxylic acid (cis-ACBC), whereas the second route is a multi-step synthesis using caprolactam as starting material.On the other hand, the (1R,2S)-cis-GABA-2,3CB was synthetized, and a series of N- and C-protected oligomers of di, tri, and tetrapeptides of this amino acid were prepared. These oligomers were characterized by NMR (1D and 2D) techniques, IR, and X-ray. The analyses have shown that there are no non-covalent interactions (hydrogen bonds) between the residues of each oligomers. However, the gelation property of these oligomers in various organic solvents was demonstrated. Solutions and gels formed from these peptides were analyzed by scanning electron microscopy, and the obtained images showed a fibrous organization of the di- and tetrapeptide, while the tripeptide showed no regular intermolecular assembly
Rezaei, Araghi Raheleh [Verfasser]. "α-helical coiled coil mimicry by alternating sequences of β- and γ-amino acids / Raheleh Rezaei Araghi". Berlin : Freie Universität Berlin, 2011. http://d-nb.info/1026069661/34.
Повний текст джерелаZimmerman, Wendy Cherie. "Association of Pericentrin with the γ Tubulin Ring Complex: a Dissertation". eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/122.
Повний текст джерелаJin, Su. "Caractéristiques physiologiques et applications de deux souches de Lactobacillus pentosus dans des produits laitiers." Phd thesis, AgroParisTech, 2010. http://pastel.archives-ouvertes.fr/pastel-00552270.
Повний текст джерелаTercinier, Pierre. "Etude de la préparation et de la réactivité des 2,3-aziridino-γ-lactones : approche à la synthèse de l’apto, acide ß-amine composant des microsclérodermines C et D". Paris 11, 2007. http://www.theses.fr/2007PA112080.
Повний текст джерелаThe work described in this thesis concems the synthesis and reactiviy of chiral bicyclic compounds, 2,3gamma-Iactones, and their use in the preparation ofpolysubstituted beta-amino acids. The reactivity of 2,3-aziridino-gamma-Iactones with soft carbon nucleophiles such as organocuprates was first studied. Different aziridines were prepared and reacted with various carbon nucleophiles. A methyl group was successfully introduced at the C-2 position but this was accompanied by the formation of many by-products. The 2,3-aziridino-gamma-Iactones were then used to synthesize APTO, a polysubstituted beta-amino acid fragment of microsclerodermines C and D. An enantiospecific synthesis of a cyclic precursor of APTO was developed starting from L-gulose. This strategy is based upon the preparation of the appropriate aziridine-Iactone, followed by regioselective CC2 ring-opening by an acetate and a Heck reaction. This intermediate was successfully opened by various amines, demonstrating its synthetic potential. Lastly, a new process for the preparation of 2,3-aziridino-gamma-Iactones starting from an unsaturated sulfamate was studied. This intramolecular nitrene transfer aziridination was catalyzed by rhodium salts and mediated by iodosylbenzene diacetate. This new methodology allowed the preparation of 2,3-aziridino-gamma-Iactones with a different stereochemistry than those synthesized previously, potentially leading to new beta-amino acids
Gozalo, Sara. "The Role of γс Cytokines in T Cell Development, T Cell Homeostasis and CD8+ T Cell Function: A Dissertation". eScholarship@UMMS, 2004. https://escholarship.umassmed.edu/gsbs_diss/140.
Повний текст джерелаPellet, Alain. "Réactivité des pyridylacétonitrile, aminonitrile et éther de cyanhydrine vis à vis de composés carbonylés α, β [alpha-béta] éthyléniques : synthèse et stéréochimie de γ [gamma] cétonitriles et de γ [gamma] cétoaldéhydes". Paris 11, 1988. http://www.theses.fr/1988PA112130.
Повний текст джерелаThis work deals with the regioselectivity of addition of alpha nitrile carbanions on unsaturated carbonyl compounds in the aim of synthesizing gamma functionalized carbonyl compounds. In the first chapter, we have shawn that the addiion of lithiated 3-pyridylacetonitrile to various enones in tetrahydrofurane, followed by protonation, leads exclusively to 1,4 adducts, the bicylcic ketones are Cis ring fused. The cis or trans stereochemistry of the 2,3-disubstituted cyclanones depends on the protonation of enolates under kinetic or thermodynamic control. The 1,2 addition of this anion on 3-methyl 2-butene 1-al is performed in tetrahydrofurane whereas 1,4 addition is observed under thermodynamic control in the presence of hexamethylphosphoramide or trifluoroboron etherate. In the second chapter, we have realized regioselective additions of lithiated and potassiated dimethylaminophenylacetonitrile and lithiated (ethoxy-ethoxy) phenylacetonitrile (latent benzoyl equivalents) on various unsaturated aldehydes. Our study centers on the influence of the nature of the reactants, solvents, cations, Lewis acids, and experimental conditions on the regio and stereoselectivities of these additions. A methodology leading to ketoaldehydes and keto allylic alcohols, usually difficult to obtain, was improved and applied to the synthesis of optically active compounds. In the third chapter, the reductive decyanation by different borohydrides was studied on an aminonitrile model with the aim of accessing to aminoaldehydes and aminoallylic alcohols. The desired free amine is accompanied by various amounts of stable amine-borane complexe according to the reducing agent. This methodology, applied to 1,4 adducts, leads to the corresponding aminoalcohols while retrocondensation occurs from the1,2 adducts
El, Khoueiry Corinne. "Propriétés morphologiques et électrophysiologiques des interneurones PKCγ de la couche IIi du Sp5C chez le rat". Thesis, Clermont-Ferrand 1, 2015. http://www.theses.fr/2015CLF1DD02.
Повний текст джерелаMechanical allodynia, a cardinal symptom of persistent pain, is associated with the unmasking of usually blocked local circuits within the superficial spinal or medullary dorsal horn (MDH), through which low-threshold mechanical inputs can gain access to the lamina I nociceptive output neurons. Key determinants of these circuits are lamina II (IIi) excitatory interneurons that selectively concentrate the gamma isoform of protein kinase C (PKCγ) and receive low-threshold mechanical receptor (LTMR) inputs. Tonic inhibition of PKCγ interneurons is thought to gate circuits underlying mechanical allodynia. However, the morphology, electrophysiological properties and excitatory and inhibitory synaptic inputs on these PKCγ interneurons are still unknown. Using whole-cell patch-clamp recordings and immunohistochemical techniques in slices of adult rat MDH, we characterized these lamina IIi PKCγ interneurons and compared them with neighboring non-PKCγ interneurons. Our results reveal that the neurites of PKCγ interneurons arborize extensively within lamina IIi, can spread dorsally into lamina IIo, but never reach lamina I. In addition, according to cell bodies and the orientation and extent of dendritic arbors, at least two morphologically different classes of PKCγ interneurons can be identified – central and radial – which appear to be also functionally different. Compared with neighboring lamina IIi non-PKCγ interneurons, PKCγ interneurons exhibit a lower threshold for action potentials, consistent with a more frequent tonic spike discharge to depolarizing step current, indicating that they are more excitable than other lamina IIi neurons. On the other hand, they receive a weaker excitatory synaptic drive. According to inhibitory inputs, most PKCγ interneurons display mixed-GABAA (GABAAR) and glycine (GlyR) receptor synapses with only very few of them displaying also GABAAR-alone or GlyR-alone synapses. Interestingly, all PKCγ interneurons exhibit mixed GABAAR–GlyR as well as GABAAR-only and GlyR-only mIPSCs. Altogether, this study indicates that PKCγ interneurons within lamina IIi of MDH are different from other lamina IIi neighboring neurons according to morphology, electrophysiological properties and synaptic inputs. This is consistent with their specific role in the gating of dorsally directed circuits within the MDH underlying mechanical allodynia. Moreover, we have identified two morphological and functional subclasses of PKCγ interneurons which might thus differently contribute to this gating
Lin, Jueh Feng, та 林玥妦. "Physiology Functional Application of γ-Amino Butyric Acid - A Review". Thesis, 2012. http://ndltd.ncl.edu.tw/handle/85125824216899718142.
Повний текст джерела中華醫事科技大學
生物醫學研究所
100
GABA is the chief inhibitory neurotransmitter in the mammalian central nervous system. It plays a role in regulating neuronal excitability throughout the nervous system. Many studies have showed that GABA was involved in many physiological functions. (1) Activated GABA receptor induces vasodilation and angiotensin converting enzyme (ACE) inhibition. The blood vessel relaxation leads to decrease blood pressure. (2) It is well established that activation of GABAA receptors favors sleep. The GABAA receptor-mediated inhibitory processes decrease waking and increase slow-wave sleep and the paradoxical sleep is decreased by agonistic modulators of GABAA receptors. (3) Benzodiazepine-induced conformational changes increase GABA's receptor affinity, and thus increase the frequency of ion channel openings. Chloride ion influx hyperpolarizes GABA neurons and produces GABA's inhibitory interneuronal effects. (4) Adding GABA and GABA agonists improve the function of cells in the visual cortex (area V1) and facilitate visual function in old animals. (5) The activation of GABAA receptor leading to Ca2+ channels opening are involved in progesterone-induced acrosomal exocytosis in mouse spermatozoa. (6)To inhibit the inflammatory response and stimulate the epidermal revive. (7)GABA receptors control gastrointestinal motility. (8) Be supplement feed to make domestic birds and animail health. Many Lactic acid bacterium (LAB) studies found, these beneficial effects not only came from bacterium itself but also metabolism products, including lactic acid, bacteriocin, polysaccharide and GABA. The thesis review and summarize these publications and according to these studies, we believed that GABA is worthy to develop and apply in functional food.
Hu, Ke-Hsun, та 胡恪洵. "The Processing Conditions of Phytic Acid and γ-amino Butyric Acid from Rice Bran". Thesis, 2010. http://ndltd.ncl.edu.tw/handle/99838903676651865690.
Повний текст джерела國立臺灣海洋大學
食品科學系
98
Rice bran (RB), due to contain considerable glutamate decarboxylase activity, could be used to incubate with monosodium L-glutamate (MSG) substrate to produce -aminobutyric acid (GABA). After water-soluble portion was removed, large amounts of active components such as phytic acid (PA) and oryzanol remained and is worthy of recovery. This study investigated to use RB not only for the production of GABA but also to to extract PA under appropriate processing conditions. PA amounts recovered from acetone-defatted rice bran (DRB) were 25.1~47.8% higher than that from raw RB. But it might be lose of the activity of GAD, the production of GABA using DRB decreased by 32.8%. After the incubation of raw RB and MSG at 35oC for 6 hours, the whole reactant was called rice bran cake (RBC). Among raw RB, DRB and RBC as starting material, RBC without a further centrifugation (RBC before centrifugalizing) was the best source for the recovery of GABA and PA. Factors that affecting the recovery of PA including hydrochloric acid concentration (0.1~2.0 M), extraction temperature (25~45oC) and extraction time (0~5 hr) were compared. To extract RBC before centrifugal by 2.0 M HCl for 1 hr at ambient temperature had the highest yield of PA (100.01 mg/g RB). Also yield of GABA from the subsequent recovery producedure was not affected, amounting in a range of 3,579 ± 252.10 ~ 4,377 ± 15.57 g/g and showing no significant difference with initial content. For separation of PA from acid extract, acid extract was neutralized by adding NaOH solution and this resulted in a precipitation of phytate salt. The recovery of PA, however, was only 44.7%. On the other hand, when acid extract was firstly titrated with NaOH solution to pH 3.0 and then mixed with acetate buffer (pH 3.0), the solution was applied into a Dowex 50WX8 ion-exchange column to elute GABA and PA. It was found that the PA recovery rose to 70%. Also GABA and PA were completely separated from each other. The heating of PA sample thus obtained at 120oC for 24 hr showed the lowest PA remained, suggesting that the production of inositol might be the largest.
廖笳因. "Synthesis and Pharmacological Evaluation of Amino Acid-Derived and Peptidomimetic γ-secretase Inhibitors". Thesis, 2004. http://ndltd.ncl.edu.tw/handle/60287417370818761229.
Повний текст джерела國防醫學院
藥學研究所
92
The activity of γ-secretase controls the amount of amyloid β-peptide (Aβ) formation, which is central in the pathogenesis of Alzheimer’s disease (AD). γ-Secretase has been considered central to understanding the etiology of AD because it determines the proportion of the highly fibrillogenic Aβ42 peptide. The fact that Aβ42 is increase in all forms of early-onset AD is very intriguing because this form of Aβ fibrils is toxic to cultured neurons. Therefore, γ-secretase has been one of the major goals of drug discovery for AD. In this study, we started the development of potential γ-secretase inhibitors based on two lead compounds (i) Boc-FΨF-Leu-Val-OMe, a peptidomimetic with hydroxyethyl moiety mimicking the transition-state of aspartyl protease catalysis, (ii) 3,5-difluorophenylacetyl- alanyl-(S)-phenylglycine t-butyl ester (DAPT), an amino acid derivative. Moieties on P1’ and P3’ positions of Boc-FΨF-Leu-Val-OMe and difluorophenylacetyl and alanyl groups of DAPT were the focus of the series of chemical optimizations, respectively. An assay that expresses a Gal4-VP16 (GVP) tagged APP or C99 and a Gal4-promoter luciferase reporter gene were established to monitor the inhibitory potencies for the series of compounds against γ-secretase activity. The results indicated that hydroxyethylureas 23c, 23d, 23e, 23f, 24a, 24b and 24c and amino acid derivative 47 possess the similar or higher activity as compared with Compound E, whereas compounds derived from modification of difluorophenylacetyl and alanyl groups at DAPT dramatically decreased the inhibitory potency against γ-secretase.
George, Gijo. "Structural Insights into Peptide Foldamers Containing β or γ Amino Acid Residues Gained Using NMR Spectroscopy". Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5210.
Повний текст джерелаCSIR
chuan, yang sheng, та 楊盛全. "β-amino-α,β-unsaturated,γ-thiolactone's Diels-Alder reaction and Lewis acid catalyze Michael addition". Thesis, 1999. http://ndltd.ncl.edu.tw/handle/59276036791545422650.
Повний текст джерела朝陽大學
應用化學研究所
87
We build our lab's method to synthesize β-amino-α,β-unsaturated,γ-thiolactone 1 (achiral)、2 (chiral), and have good yield. From thiolactone 1、2, we have to synthesize thiophene 3、4, and study thiophene's Diels-alder reaction and Lewis acid catalyze Michael addition
Lin, Chang-Ching, та 林昌慶. "Aldol reaction Studies of β-amino α,β-unsaturated γ-thiolactone under Basic and Lewis acid conditions". Thesis, 2000. http://ndltd.ncl.edu.tw/handle/84056287302188170852.
Повний текст джерела朝陽大學
應用化學系碩士班
88
A novel procedure for the synthesis of achiral thiopenone from Methyl 3-pyrrolidino(E)-2-butenoate and it’s application on the studies of Aldol reactions under basic(including the temperature,counter ion , base reagent effect) and Lewis acid conditions (Lewis such as BF3.Et2 , SnCl4 , TiCl4) to generate syn - product or anti-product will be discussed.
CHEN, BO-KAI, та 陳柏凱. "Screening High Glutamate Decarboxylase Activity from Germinated Soybean to Optimize Production Conditions for γ-amino butyric acid". Thesis, 2017. http://ndltd.ncl.edu.tw/handle/pmsuy7.
Повний текст джерела東海大學
食品科學系
105
The aim of this study was to screen soybeans cultured in Taiwan with highest glutamate decarboxylase (GAD) in their sprouts. The optimum conditions for the production of Gamma-amino butyric acid (GABA) were also studied by extracting GAD crude enzyme from the selected soybean sprouts. First of all, sprouts of black soybean Tainan 3, black soybean Tainan 5, Jinzhu soybean and Kaohsiung 10, were prepared under 27℃, lightless and every 2 hrs automatic sprinkler culture. The results showed that GAD activity in all cultivars increased with the germination time. GAD enzyme in each breed of bean sprouts can reach maximum activity within 10 days. Black soybean Tainan 3 had its best enzyme activity on the 3rd day and 7-9th days. Both black soybean Tainan 5 and Jinzhu soybean showed their best enzyme activity on the 7th day. Kaohsiung 10 revealed its best enzyme activity during the 3-10th days, The maximum GAD activity in various varieties was between 2.20 and 3.41 U. But there was no significant difference between them (p <0.01). Therefore, sprouts of Kaohsiung 10 were selected for further experiments, because of its shortest required germination time and relative higher GAD activity. Furthermore, response surface methodology was applied to optimize the independent variables including reaction temperature 28.6~75.2℃, pH 5.32~8.68 and glutamate concentration 3.2~36.8 mM for GABA production. Based on response surface regression analysis (RSREG) procedure, the optimum reaction conditions for maximizing GABA production of 601 mg/L were 57.5 ℃, pH 7.75 and 27.5 mM glutamate. The results showed that the highest yield of GABA production could be up to 1027.49 mg/L when the reaction time was extended to 6 hrs. Finally, it were verified by the conversion rate. The highest conversion of glutamate to GABA by GAD enzyme was also carried out in 6 hrs.
Hou, Chien-Chih, та 侯建志. "Study of the Possibilities of Using Soybeans Rich in γ-Amino Butyric Acid to Produce Soybean Milk". Thesis, 2007. http://ndltd.ncl.edu.tw/handle/58892854035278442218.
Повний текст джерела國立屏東科技大學
生物機電工程系所
95
The general populace starts to take care of their health in recent years. Therefore, the acceptation regarding to high nutritional value food gradually become popular. However, the soybean is the Chinese peoples' often edible food. Soybean itself has includes the extremely high GABA(γ-Amino butyric acid), GABA was proved at many experiments that soybean has many effects. The physiology function like stable spirit, the improvement of arteriosclerosis, the improvement of losing sleep, the suppression of blood pressure rise, to promotes the brain metabolism, to promote the memory, the prevention arteriosclerosis, the activation kidney function and so on. Germinates is an important process which the plant grows, when the new bud sprouts it can activate the sleep enzyme, as increase growth needs nutrient. According to the literature pointed out germinates truly can cause GAD(glutamate decarboxylase) to escape. From the activation it has increase GABA, but this research is then urges using the soybean natural growth way to stimulates soybean's GABA increase, in order to use it to manufacture the soybean milk. Firstly, to germinate the soybean under the following environment, by the temperature 30℃ and humidities 75%. Then, uses HPLC(high peformance liquid chromatograph) to analysis. Meter to examines the soybean sample's quantity contained for GABA and with bud length between relations. The experimental result showed, using the way which germinates may cause the originally not germinated soybean's GABA content to enhance from 1289 mg/100g to rise to the germinates 24 hour after 1729 mg/100g, its content increases 34.1%. When the bud length is 16.1 ~ 23.3 mm, GABA all presents condition of the increase. Nevertheless, after makes the soybean milk, the not germinated soybean milk and which the soybean does with to germinate 24 hours's soybean milk its GABA content, respectively is 70 mg/100ml and 83 mg/100ml. However, when it is in the use of N-hexane in degreasing experiment, by 100℃ steams the soybean milk which has not germinated, Its increase normal hexane may make the GABA content to increase from 70 mg/100g to 84 mg/100g. However, by 100℃ steams germinates 24 hours soybean milk, Its increase normal hexane may make the GABA content to increase from 83 mg/100g to 96 mg/100g. In conclusion, the use of germinates way truly may cause to increase GABA in the soybean. However, when manufactures under the soybean milk ,it is also to be allowed to retain more than 70% above GABA not to be able to destroy during the soybean milk manufacture process. Therefore, uses of germinates the soybean in order to use in to manufacture the soybean milk, it does have the high feasibility.
Cheng, Chiao-Hsin, та 陳巧馨. "Study on optimized production conditions for γ-amino butyric acid using glutamate decarboxylase from germinated Adzuki bean". Thesis, 2011. http://ndltd.ncl.edu.tw/handle/91963148339810797248.
Повний текст джерела東海大學
食品科學系
100
Abstract The aim of this study was to investigate the optimum production conditions of γ-amino butyric acid (GABA) by glutamate decarboxylase (GAD) from germinated Adzuki bean. Response surface methodology was applied to evaluate the independent variables including reaction temperature 28.6~45.5℃, pH 6.32~9.68 and PLP concentration 0.032~0.368 mM under fixed enzyme to substrate ratio of 1:75, 0.25 mM CaCl2 and reaction time 1h. Based on response surface regression analysis (RSREG) procedure, the optimum reaction conditions for maximizing GABA production of 326.7 mg/L were 37.2 ℃, pH 7.6 and 0.208 mM PLP. The maximum predicted value could be achieved whin 95% confidence interval of experimental values. Furthermore, bioreactor was employed to convert glutamate with GAD into GABA and to recycle the GAD. The productivity of bioreactor was 1.663 mg GABA/mg protein, which was three fold than that of batch reactor after reusing the GAD for three times. The molecular weight of the GAD from germinated Adzuki bean was about 101 kDa (tetramer). Based on enzymatic kinetic study, the parameters of km and Vmax were 16.66 mM and 555 mg /L, respectively. The result of storage stability test indicated that 70% GAD activity was maintained after a half year storage under both 4 ℃and -20 ℃.
Church, A. T., Zak E. Hughes та T. R. Walsh. "Improving the description of interactions between Ca2+ and protein carboxylate groups, including γ–carboxyglutamic acid: revised CHARMM22* parameters". 2015. http://hdl.handle.net/10454/15911.
Повний текст джерелаA reliable description of ion pair interactions for biological systems, particularly those involving polyatomic ions such as carboxylate and divalent ions such as Ca2+, using biomolecular force-fields is essential for making useful predictions for a range of protein functions. In particular, the interaction of divalent ions with the double carboxylate group present in γ-carboxyglutamic acid (Gla), relevant to the function of many proteins, is relatively understudied using biomolecular force-fields. Using force-field based metadynamics simulations to predict the free energy of binding between Ca2+ and the carboxylate group in liquid water, we show that a widely-used biomolecular force-field, CHARMM22*, substantially over-estimates the binding strength between Ca2+ and the side-chains of both glutamic acid (Glu) and Gla, compared with experimental data obtained for the analogous systems of aqueous calcium–acetate and calcium–malonate. To correct for this, we propose and test a range of modifications to the σ value of the heteroatomic Lennard–Jones interaction between Ca2+ and the oxygen of the carboxylate group. Our revised parameter set can recover the same three association modes of this aqueous ion pair as the standard parameter set, and yields free energies of binding for the carboxylate–Ca2+ interaction in good agreement with experimental data. The revised parameter set recovers other structural properties of the ion pair in agreement with the standard CHARMM22* parameter set.
Basuroy, Krishnayan. "Structural Studies on Hybrid Peptides : Crystallographic Characterization of Sequences Containing Backbone Homologated, Monosubstituted, Unconstrained Gamma Amino Acid Residues." Thesis, 2013. https://etd.iisc.ac.in/handle/2005/4578.
Повний текст джерелаΑντωνίου, Αντωνία. "Σύνθεση μη-πρωτεϊνικών αμινοξέων για εφαρμογές τους στην πεπτιδική σύνθεση σε στερεή φάση". Thesis, 2012. http://hdl.handle.net/10889/6083.
Повний текст джерелаIn the present dissertation a new methodology is reported for the synthesis of novel non-proteinogenic amino acids (NPAAs) suitable for applications in solid phase peptide synthesis. This methodology involves aspartic acid as chiral template and relies on the successful and in good yields pepraration of the key N-tritylaspartic tert-butyl ester’s β-aldehyde, which when followed by Wittig, Horner-Emmons or Grignard type reactions may result in a variety of new NPAAs. Furthermore unsuccessful attempts towards the application of the present methodology for the synthesis of NPAA homologues bearing one carbon atom less or more in the side chain by using as chiral templates serine or glutamic acid, respectively, are also reported.
Kleeberg, Kim Karen [Verfasser]. "Identifizierung von Aminosäure-Radiolyseprodukten und sensorische Veränderungen in γ-bestrahlten [gamma-bestrahlten] Nordseegarnelen (Crangon crangon) und anderen eiweißreichen Lebensmitteln = Identification of amino acid radiation products and sensory effects on γ-irradiated [gamma-irradiated] brown shrimps (Crangon crangon) and other protein rich foods / Kim Karen Kleeberg". 2002. http://d-nb.info/966133439/34.
Повний текст джерелаHuang, Chih-Hao, та 黃志浩. "Enantioselective Synthesis of γ-Amino Acids and γ-Amino Phosphonic Acids via Rhodium Catalyzed Asymmetric 1,4-Conjugate Addition Reactions". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/98474602709529973873.
Повний текст джерелаGade, Kishore. "Synthesis of Glycosyl Amino Acids, Glyco Amino Acids & α-Amino γ-Lactams from Carbohydrate Derived Donor-Acceptor Cyclopropanes". Thesis, 2014. http://etd.iisc.ac.in/handle/2005/2779.
Повний текст джерелаGade, Kishore. "Synthesis of Glycosyl Amino Acids, Glyco Amino Acids & α-Amino γ-Lactams from Carbohydrate Derived Donor-Acceptor Cyclopropanes". Thesis, 2014. http://etd.iisc.ernet.in/handle/2005/2779.
Повний текст джерелаEarle, Marion. "Carbohydrate auxiliaries for the asymmetric synthesis of α-amino acids and γ-oxo-α-amino acids". 2002. http://hdl.handle.net/1993/18896.
Повний текст джерелаJian, Jia-Hong, та 簡嘉宏. "Enantioselective Synthesis of γ-Amino Acids Via a Rhodium Catalyzed Asymmetric 1,4-conjugate Addition Reaction of γ-Amino-Substituted α , β- Unsaturated Esters and Arylboronic Acids". Thesis, 2016. http://ndltd.ncl.edu.tw/handle/27121472293967636504.
Повний текст джерелаHaque, Mohammad Mahbubul [Verfasser]. "Enantioselective synthesis of new conformationally constrained sugar-like γ- [gamma-], δ- [delta-], {ε-amino [epsilon-amino] acids, {δ-peptides [delta-peptides] and nucleoside amino acids / vorgelegt von Mohammad Mahbubul Haque". 2006. http://d-nb.info/982215967/34.
Повний текст джерелаRahem, Neel. "Synthèses de précurseurs carbonylés γ, δ - insaturés via un réarrangement de Claisen [3,3] : vers une nouvelle voie de synthèses d'acides aminés non naturels". Mémoire, 2010. http://www.archipel.uqam.ca/3394/1/M11487.pdf.
Повний текст джерелаGuitot, Karine [Verfasser]. "Synthesis and conformational analysis of peptidomimetics containing unnatural amino acids based on a piperazinone and γ-butyrolactone [gamma-butyrolactone] scaffolds / vorgelegt von Karine Guitot". 2008. http://d-nb.info/1004102666/34.
Повний текст джерелаVasudev, Prema G. "X-Ray Crystallographic Studies Of Designed Peptides : Characterization Of Novel Secondary Structures Of Peptides Containing Conformationally Constrained α-, β- And γ-Amino Acids And Polymorphic Peptide Helices". Thesis, 2009. https://etd.iisc.ac.in/handle/2005/922.
Повний текст джерелаVasudev, Prema G. "X-Ray Crystallographic Studies Of Designed Peptides : Characterization Of Novel Secondary Structures Of Peptides Containing Conformationally Constrained α-, β- And γ-Amino Acids And Polymorphic Peptide Helices". Thesis, 2009. http://hdl.handle.net/2005/922.
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