Статті в журналах з теми "¹⁵N Milk proteins"
Щоб переглянути інші типи публікацій з цієї теми, перейдіть за посиланням: ¹⁵N Milk proteins.
Оформте джерело за APA, MLA, Chicago, Harvard та іншими стилями
Ознайомтеся з топ-50 статей у журналах для дослідження на тему "¹⁵N Milk proteins".
Біля кожної праці в переліку літератури доступна кнопка «Додати до бібліографії». Скористайтеся нею – і ми автоматично оформимо бібліографічне посилання на обрану працю в потрібному вам стилі цитування: APA, MLA, «Гарвард», «Чикаго», «Ванкувер» тощо.
Також ви можете завантажити повний текст наукової публікації у форматі «.pdf» та прочитати онлайн анотацію до роботи, якщо відповідні параметри наявні в метаданих.
Переглядайте статті в журналах для різних дисциплін та оформлюйте правильно вашу бібліографію.
1
Hernández-Caravaca, Iván, Andrés Cabañas, Rebeca López-Úbeda, Leopoldo González-Brusi, Ascensión Guillén-Martínez, Mª José Izquierdo-Rico, Mª Nieves Muñoz-Rodríguez, Manuel Avilés, and Mª Jesús Ruiz García. "Analysis of Minor Proteins Present in Breast Milk by Using WGA Lectin." Children 9, no. 7 (July 20, 2022): 1084. http://dx.doi.org/10.3390/children9071084.
Анотація:
Breast milk is a complex and dynamic biological fluid and considered an essential source of nutrition in early life. In its composition, the proteins have a relevant biological activity and are related to the multiple benefits demonstrated when compared with artificial milks derived from cow’s milk. Understanding human milk composition provides an important tool for health care providers toward the management of infant feeding and the establishment of breastfeeding. In this work, a new technique was developed to increase the knowledge of human milk, because many of the components remain unknown. To isolate minor proteins present in breast milk by using WGA lectin, breast milk was centrifuged to remove cells and separate the fat phase from the serum phase. The serum obtained was separated into two groups: control (n = 3; whole serum sample from mature milk) and WGA lectin (n = 3; sample processed with WGA lectin to isolate glycosylated proteins). The samples were analyzed by high-performance liquid chromatography coupled to mass spectrometry (HPLC/MS). A total of 84 different proteins were identified from all of the samples. In the WGA lectin group, 55 different proteins were isolated, 77% of which had biological functions related to the immune response. Of these proteins, there were eight WGA lectin group exclusives, and two had not previously been described in breast milk (polyubiquitin-B and POTE ankyrin domain family member F). Isolation by WGA lectin is a useful technique to detect minor proteins in breast milk and to identify proteins that could not be observed in whole serum.
2
Scuderi, Richard A., David B. Ebenstein, Ying-Wai Lam, Jana Kraft, and Sabrina L. Greenwood. "Inclusion of grape marc in dairy cattle rations alters the bovine milk proteome." Journal of Dairy Research 86, no. 2 (May 2019): 154–61. http://dx.doi.org/10.1017/s0022029919000372.
Анотація:
AbstractGrape marc (GPM) is a viticulture by-product that is rich in secondary compounds, including condensed tannins (CT), and is used as a supplement in livestock feeding practices. The aim of this study was to determine whether feeding GPM to lactating dairy cows would alter the milk proteome through changes in nitrogen (N) partitioning. Ten lactating Holstein cows were fed a total mixed ration (TMR) top-dressed with either 1.5 kg dry matter (DM)/cow/day GPM (GPM group; n = 5) or 2.0 kg DM/cow/day of a 50:50 beet pulp: soy hulls mix (control group; n = 5). Characterization of N partitioning and calculation of N partitioning was completed through analysis of plasma urea-N, urine, feces, and milk urea-N. Milk samples were collected for general composition analysis, HPLC quantification of the high abundance milk proteins (including casein isoforms, α-lactalbumin, and β-lactoglobulin) and liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the low abundance protein enriched milk fraction. No differences in DMI, N parameters, or calculated N partitioning were observed across treatments. Dietary treatment did not affect milk yield, milk protein or fat content or yield, or the concentrations of high abundance milk proteins quantified by HPLC analysis. Of the 127 milk proteins that were identified by LC-MS/MS analysis, 16 were affected by treatment, including plasma proteins and proteins associated with the blood-milk barrier, suggesting changes in mammary passage. Immunomodulatory proteins, including butyrophilin subfamily 1 member 1A and serum amyloid A protein, were higher in milk from GPM-fed cows. Heightened abundance of bioactive proteins in milk caused by dietary-induced shifts in mammary passage could be a feasible method to enhance the healthfulness of milk for both the milk-fed calf and human consumer. Additionally, the proteome shifts observed in this trial could provide a starting point for the identification of biomarkers suitable for use as indicators of mammary function.
3
Ruprichová, Lenka, Michaela Králová, Ivana Borkovcová, Lenka Vorlová, and Iveta Bedáňová. "Determination of whey proteins in different types of milk." Acta Veterinaria Brno 83, no. 1 (2014): 67–72. http://dx.doi.org/10.2754/avb201483010067.
Анотація:
Protein analysis is very important both in terms of milk protein allergy, and of milk and dairy product adulteration (β-lactoglobulin may be an important marker in the detection of milk adulteration). The aim of this study was to detect major whey proteins α-lactalbumin and β-lactoglobulin and their genetic variants by reversed-phase high-performance liquid chromatography. Milk samples from cows (n = 40), goats (n = 40) and sheep (n = 40) were collected at two farms and milk bars in the Czech Republic from April to June 2010. The concentration of α-lactalbumin was higher in goat’s milk (1.27 ± 0.05 g·l-1, P < 0.001) and cow’s milk (1.16 ± 0.02 g·l-1, P = 0.0037) compared to sheep’s milk (0.95 ± 0.06 g·l-1); however, concentration of α-lactalbumin in goat’s milk and cow’s milk did not differ significantly (P < 0.05). Goat’s milk contained less β-lactoglobulin (3.07 ± 0.08 g·l-1) compared to cow’s milk (4.10 ± 0.04 g·l-1, P < 0.001) or sheep’s milk (5.97 ± 0.24 g·l-1, P < 0.001). A highly significant positive correlation (r = 0.8686; P < 0.001) was found between fraction A and B of β-lactoglobulin in sheep’s milk, whereas in cow’s milk there was a negative correlation (r = -0.3010; P = 0.0296). This study summarizes actual information of the whey protein content in different types of milk which may be relevant in assessing their allergenic potential.
4
Cunha, Inês Machado, Ana Raquel Pinto, Borja Bartolomé, and Helena Falcão. "Food Allergy to Sheep’s Milk Proteins with Cow’s Milk Tolerance in an Adult Patient." Acta Médica Portuguesa 36, no. 1 (January 2, 2023): 68–69. http://dx.doi.org/10.20344/amp.19153.
5
Chow, Brian D. W., Juliann L. Reardon, Emily O. Perry, Sonia S. Laforce-Nesbitt, Richard Tucker, and Joseph M. Bliss. "Host Defense Proteins in Breast Milk and Neonatal Yeast Colonization." Journal of Human Lactation 32, no. 1 (June 26, 2015): 168–73. http://dx.doi.org/10.1177/0890334415592402.
Анотація:
Background: Colonization increases risk for invasive candidiasis in neonates. Breast milk host defense proteins may affect yeast colonization of infants. Objective: This study aimed to evaluate breast milk host defense proteins relative to yeast colonization in infants. Methods: Infants admitted for longer than 72 hours to the neonatal intensive care unit at Women & Infants Hospital in Providence, Rhode Island, were eligible. After consent, expressed breast milk and swabs from oral, rectal, and inguinal sites from infants were cultured weekly for 12 weeks, or until discharge, transfer, or death. Breast milk was tested for levels of human lactoferrin, lysozyme, apolipoprotein J, mucin-1, dermcidin, and soluble CD14 using commercial ELISA. Concentrations of these components were compared in breast milk received by infants who were colonized or not colonized with yeast. Results: From an original cohort of 130, 61 infants had samples available for this subanalysis. A convenience sample of stored breast milk was analyzed. Median lactoferrin, apolipoprotein J, and mucin-1 did not differ between colonized and uncolonized groups. Soluble CD14 was higher in the surface-colonized group (1.8 μg/mL, n = 12) compared with the surface-uncolonized group (1.6 μg/mL, n = 12, P = .02). Median lysozyme levels were higher in the surface-uncolonized group (483.0 ng/mL, n = 12) versus the surface-colonized group (298.3 ng/mL, n = 12, P = .04). Median dermcidin levels were higher in the surface-uncolonized group (19.4 ng/mL, n = 12) versus the surface-colonized group (8.7 ng/mL, n = 12, P = .04). Conclusion: This study shows an association between colonization with Candida in neonates and lower levels of lysozyme and dermcidin in received breast milk. Further study is needed to confirm these findings.
6
Schwertmann, Annette, Horst Schroten, Jörg Hacker, and Clemens Kunz. "S‐Fimbriae From Escherichia coli Bind to Soluble Glycoproteins From Human Milk." Journal of Pediatric Gastroenterology and Nutrition 28, no. 3 (March 1999): 257–63. http://dx.doi.org/10.1002/j.1536-4801.1999.tb02060.x.
Анотація:
ABSTRACTBackground:Escherichia coli (E. coli) strains, expressing S‐fimbriae, belong to the most common gram‐negative pathogens that cause sepsis and meningitis in neonates. The attachment of S‐fimbriae to the cell surface is mediated by membrane glycoconjugates, which often carry N‐acetylneuraminic acid.Methods:Binding studies were performed with glycoproteins from the whey fraction of human milk to investigate whether they exert a potential inhibitory effect on bacterial adhesion. Whey glycoproteins were separated according to their molecular weight by fast protein liquid chromatography gel filtration. After sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, proteins were transferred to nitrocellulose membranes and incubated with isolated S‐fimbriae from recombinant E. coli strain HB (pANN 801‐4).Results:S‐fimbriae recognized four whey proteins with a molecular mass of more than 200 kDa, 170 to 150 kDa, and 80 kDa. Their glycosylation pattern was investigated using the lectins Sambucus nigra, Maackia amurensis, Galanthus nivalis, and Arachis hypogaea. Thus the presence of N‐ and O‐glycans in these proteins was confirmed. The preferential binding to N‐acetylneuraminic acid containing glycoproteins was demonstrated by a complete abolishment of these reactions by incubation with acidic lactose‐derived oligosaccharides. However, the cleavage of N‐acetylneuraminic acid from glycoproteins by mild acid hydrolysis revealed a second binding site for S‐fimbriae on milk proteins of a similar molecular weight range. Terminal galactose in human milk glycoconjugates were thought to react with S‐fimbriae as well.Conclusion:These data further support the opinion that glycoproteins from human milk are potential receptor analogues for certain bacteria that may prevent microbial adhesion to the epithelial cell surface.
7
Filgueira, Luis, Gwendoline Kueffer, Alecia-Jane Twigger, Solange Kharoubi, Michael Walch, and Donna T. Geddes. "Antimicrobial Cytotoxic Immune Proteins in Human Milk." Journal of Immunology 198, no. 1_Supplement (May 1, 2017): 200.3. http://dx.doi.org/10.4049/jimmunol.198.supp.200.3.
Анотація:
Abstract Human milk (HM) contains a large variety of immune components providing protection to the infant. The presence of the cytotoxic immune proteins perforin (Per), granulysin (Grly)and granzymes (Grzm)has not yet been shown in HM cells. Per, Grnly and Grzm (A, B, H and M) were examined in cells isolated from samples of prepartum HM (PS) collected during pregnancy and HM collected longitudinally (2–5 sampling occasions) from mothers (n=31) during the first year postpartum, including 3 samples from breast infections. Gene expression was analyzed in RNA sequencing data and using qRT-PCR. Linear modeling and principle component analysis between the genes was conducted. Flow cytometry (n=5) was performed to investigate the presence of these immune proteins in HM cells. Gene expression of all immune proteins was confirmed in PS and in HM during month 1 of lactation, where variation of expression was found between women and time lactation. Strong correlations were found between GrzmA – Per (r2=0.86), GrzmA – CD45 (r2=0.76), Per – CD45 (r2=0.68) and Per – GrzmB (r2=0.67). Comparison between healthy and mastitis samples showed a higher expression of CD45 and all immune peptides in mastitis. Moreover, flow cytometry analysis showed an increase in CD45 positive cells in mastitis in comparison with healthy participants (15.1% and 4.7% respectively of total cell amount). The presence of Per, Grly and Grzm has been confirmed in HM cells. An increase in gene expression of these immune proteins has been confirmed in 3 participants suffering from breast infection. Further investigations are required to elucidate the roles of these immune peptides for the infant and/or mother
8
Beg, Obaid Ullah. "Partial Characterization of Platypus (Ornithorhynchus Anatinus) Milk Proteins." Protein & Peptide Letters 2, no. 3 (December 1995): 431–34. http://dx.doi.org/10.2174/092986650203220603093448.
Анотація:
The purification of milk whey proteins of Platypus (Ornithorhynchus anatinus) by reverse phase high performance liquid chromatography is presented.The amino acid compositions and N-terminal sequences of purified proteins were determined and the proteins were identified as cysteine rich-whey acidic protein, f3- lactoglobulin and galactosyltransferase.
9
Wilde, C. J., C. V. P. Addey, L. M. Boddy, and M. Peaker. "Autocrine regulation of milk secretion by a protein in milk." Biochemical Journal 305, no. 1 (January 1, 1995): 51–58. http://dx.doi.org/10.1042/bj3050051.
Анотація:
Frequency or completeness of milk removal from the lactating mammary gland regulates the rate of milk secretion by a mechanism which is local, chemical and inhibitory in nature. Screening of goat's milk proteins in rabbit mammary explant cultures identified a single whey protein of M(r) 7600 able to inhibit synthesis of milk constituents. The active whey protein, which we term FIL (Feedback inhibitor of Lactation), also decreased milk secretion temporarily when introduced into a mammary gland of lactating goats. FIL was synthesized by primary cultures of goat mammary epithelial cells, and was secreted vectorially together with other milk proteins. N-terminal amino acid sequencing indicated that it is a hitherto unknown protein. The evidence indicates that local regulation of milk secretion by milk removal is through autocrine feedback inhibition by this milk protein.
10
Dupont, Didier, and StÉphanie Muller-Renaud. "Quantification of Proteins in Dairy Products Using an Optical Biosensor." Journal of AOAC INTERNATIONAL 89, no. 3 (May 1, 2006): 843–48. http://dx.doi.org/10.1093/jaoac/89.3.843.
Анотація:
Abstract Two recent techniques using optical immunosensor technology were developed for the quantification of milk proteins in dairy products. The first application is the simultaneous quantification of the 3 major caseins (αS1, β, and κ). This assay consists of a 2-step sandwich strategy, with 2 monoclonal antibodies directed against the N- and C-terminal extremities of each of the caseins, respectively. This strategy permits only intact caseins to be quantified, and not their degradation products. The technique is fast (10 min), sensitive (detection limit about 0.87 μg/mL), and has been applied successfully to raw and drinking milks. In the second application, the severity of the heat treatment sustained by a milk of unknown origin is determined by quantifying separately the native and heat-denatured forms of α-lactalbumin with specific monoclonal antibodies. The technique allows discrimination of the different heat treatments studied (pasteurization, direct and indirect ultra-high temperature, sterilization), is fast (4 min), repeatable, fully automated, and requires no pretreatment of the milk sample.
11
Cao, Xueyan, Shimo Kang, Mei Yang, Weixuan Li, Shangyi Wu, Hongjiao Han, Lingshuai Meng, Rina Wu, and Xiqing Yue. "Quantitative N-glycoproteomics of milk fat globule membrane in human colostrum and mature milk reveals changes in protein glycosylation during lactation." Food & Function 9, no. 2 (2018): 1163–72. http://dx.doi.org/10.1039/c7fo01796k.
12
Goonatilleke, Elisha, Jincui Huang, Gege Xu, Lauren Wu, Jennifer T. Smilowitz, J. Bruce German, and Carlito B. Lebrilla. "Human Milk Proteins and Their Glycosylation Exhibit Quantitative Dynamic Variations during Lactation." Journal of Nutrition 149, no. 8 (May 16, 2019): 1317–25. http://dx.doi.org/10.1093/jn/nxz086.
Анотація:
ABSTRACTBackgroundProteins in human milk are essential and known to support the growth, development, protection, and health of the newborn. These proteins are highly modified by glycans that are currently being recognized as vital to protein structure, stability, function, and health of the intestinal mucosa. Although milk proteins have been studied, the quantitative changes in milk proteins and their respective site-specific glycosylation are unknown.ObjectiveThis study expanded the analytical tools for milk proteins and their site-specific glycosylation and applied these tools to a large cohort to determine changes in individual protein concentrations and their site-specific N-glycosylation across lactation.DesignA tandem mass spectrometry method was applied to 231 breast-milk samples from 33 mothers in Davis, California, obtained during 7 different periods of lactation. Dynamic changes in the absolute abundances of milk proteins, as well as variation in site-specific N-glycosylation of individual proteins, were quantified.Resultsα-Lactalbumin, β-casein, k-casein, and α-antitrypsin were significantly increased from colostrum to transitional milk (4.37 ± 1.33 g/L to 6.41 ± 0.72 g/L, 2.25 ± 0.86 g/L to 2.59 ± 0.78 g/L, 1.33 ± 0.44 g/L to 1.60 ± 0.39 g/L, and 0.09 ± 0.10 g/L to 0.11 ± 0.04 g/L, respectively; P < 0.002). α-Lactalbumin (37%), β-casein (9%), and lysozyme (159%) were higher in mature milk than in colostrum. Glycans exhibited different behavior. Fucosylated glycans of lactoferrin and high-mannose, undecorated, fucosylated, sialylated, and combined fucosylated + sialylated glycans of secretory immunoglobulin A increased during lactation even when the concentrations of the parent proteins decreased.ConclusionsProteins in healthy mothers vary dynamically through lactation to support the development of infants. Individual milk proteins carried unique glycan modifications that varied systematically in structure even with site specificity. The role of glycosylation in human milk proteins will be important in understanding the functional components of human milk. This trial was registered at clinicaltrials.gov as NCT01817127.
13
Delisle, J., J. F. Bernier, and G. J. Brisson. "Replacing skimmed milk powder by oat protein concentrate in milk replacers for piglets." Canadian Journal of Animal Science 71, no. 2 (June 1, 1991): 515–21. http://dx.doi.org/10.4141/cjas91-061.
Анотація:
Two experiments were conducted with 24- to 72-h-old male piglets. In exp. 1, 24 piglets were assigned to three dietary treatments. In the control diet, 100% of the dietary protein originated from low-heat skimmed milk powder. In the other diets, either 25 or 50% of the milk proteins were replaced by oat protein concentrate. In exp. 2, eight piglets were assigned to each of two diets where 100% of the dietary protein originated from skimmed milk powder and 50% of the milk proteins were replaced by oat protein concentrate. In exp. 1, dry matter intake was not affected by treatments while body weight gain declined and feed to gain ratio increased significantly (P < 0.05) when oat protein concentrate replaced 50% of the milk proteins. Similar observations were made in exp. 2 regarding dry matter intake, body weight gain and feed gain ratio although digestibility of dry matter and nitrogen was the same (P > 0.05) for both the oat and milk protein treatments. Oat protein concentrate can thus replace up to 50% of milk proteins without apparent detrimental effect on dry matter intake and N digestibility; however, growth rate will be reduced. Key words: Piglets, artificial rearing, milk replacer, skimmed milk powder, oat protein concentrate, digestibility
14
Baglieri, Agnes, Sylvain Mahe, Semia Zidi, Jean-Francois Huneau, Francois Thuillier, Philippe Marteau, and Daniel Tome. "Gastro-jejunal digestion of soya-bean-milk protein in humans." British Journal of Nutrition 72, no. 4 (October 1994): 519–32. http://dx.doi.org/10.1079/bjn19940056.
Анотація:
In order to determine how soya-bean proteins are digested and metabolized in the human intestine before colonic bacterial fermentation and to estimate their true digestibility, the gastro-jejunal behaviour of soya-bean proteins in water and in two other forms (a concentrated soya-bean-protein solution (isolate) and a drink composed of crude soya-bean proteins (soymilk)) was studied in humans. Experiments were carried out in eight healthy volunteers using a double-lumen steady-state intestinal perfusion method with polyethyleneglycol (PEG) as a non-absorbable volume marker. Gastric emptying and N and electrolyte contents of the jejunal digesta were analysed. Gastric half-emptying time (min) of the liquid phase after water ingestion (12·59 (SE 0·12)) was shorter (P< 0.05) than those for soymilk (37·74 (SE 11·57)) and isolate (36·52 (SE 11·23)). Electrolytic balances showed that for all meals, Na+, Cl−and K+were secreted when Ca2+ was efficiently absorbed from the jejunal lumen. Gastro-jejunal N absorption for isolate and soymilk were 63 and 49% respectively, and were not significantly different from one another; after water ingestion, endogenous N was estimated to be 21 mmol. An estimate of the exogenous: endogenous values for the effluents was obtained from the amino acid compositions of soymilk and effluents after water or soymilk ingestion, indicating that 70% of the total N was exogenous and 30% endogenous. Under these conditions the endogenous fraction represented 31 mmol after soymilk ingestion and the gastro-jejunal N balance indicated that 54% of the soymilk was absorbed. This finding indicates that the true gastro-jejunal digestibility of soya-bean proteins is similar to that of milk proteins.
15
LUCEY, JOHN A., MICHELLE TAMEHANA, HARJINDER SINGH, and PETER A. MUNRO. "Effect of interactions between denatured whey proteins and casein micelles on the formation and rheological properties of acid skim milk gels." Journal of Dairy Research 65, no. 4 (November 1998): 555–67. http://dx.doi.org/10.1017/s0022029998003057.
Анотація:
The effect of interactions of denatured whey proteins with casein micelles on the rheological properties of acid milk gels was investigated. Gels were made by acidification of skim milk with glucono-δ-lactone at 30°C using reconstituted skim milk powders (SMP; both low- and ultra-low-heat) and fresh skim milk (FSM). The final pH of the gels was ∼4·6. Milks containing associated or ‘bound’ denatured whey proteins (BDWP) with casein micelles were made by resuspending the ultracentrifugal pellet of heated milk in ultrafiltration permeate. Milks containing ‘soluble’ denatured whey protein (SDWP) aggregates were formed by heat treatment of an ultracentrifugal supernatant which was then resuspended with the pellet. Acid gels made from unheated milks had low storage moduli, G′, of <20 Pa. Heating milks at 80°C for 30 min resulted in acid gels with G′ in the range 390–430 Pa. The loss tangent (tan δ) of gels made from heated milk increased after gelation to attain a maximum at pH ∼5·1, but no maximum was observed in gels made from unheated milk. Acid gels made from milks containing BDWP that were made from low-heat SMP, ultra-low-heat SMP and FSM had G′ of about 250, 270 and 310 Pa respectively. Acid gels made from milks containing SDWP that were made from ultra-low-heat SMP or FSM had G′ values in the range 17–30 Pa, but gels made from low-heat SMP had G′ of ∼140 Pa. It was concluded that BDWP were important for the increased G′ of acid gels made from heated milk. Addition of N-ethylmaleimide (NEM) to low-heat reconstituted milk, to block the —SH groups, resulted in a reduction of the G′ of gels formed from heated milk but did not reduce G′ to the value of unheated milk. Addition of 20 mm-NEM to FSM, prior to heat treatment, resulted in gels with a lower G′ value than gels made from reconstituted low-heat SMP. It was suggested that small amounts of denatured whey proteins associated with casein micelles during low-heat SMP manufacture were probably responsible for the higher G′ of gels made from milk containing SDWP and from milk heated in the presence of 20 mm-NEM, compared with gels made from FSM.
16
RYCHEN, GUIDO, DIDIER MPASSI, STEPHAN JURJANZ, MICHEL MERTES, IRENE LENOIR-WIJNKOOP, JEAN MICHEL ANTOINE, and FRANÇOIS LAURENT. "15N as a marker to assess portal absorption of nitrogen from milk, yogurt and heat-treated yogurt in the growing pig." Journal of Dairy Research 69, no. 1 (February 2002): 95–101. http://dx.doi.org/10.1017/s0022029901005374.
Анотація:
Milk and yogurt constitute a major source of dietary protein. The nutritive value of dietary proteins is linked to subsequent postprandial amino acid availability in the portal blood (Rérat, 1988). Portal absorption of nutrients cannot be studied in humans, but pigs provide a valid model for studying protein digestion in humans (Rowan et al. 1994).Since stable isotopes are suitable to distinguish the exogenous from endogenous protein fraction in the intestinal lumen, intrinsic isotopic labelling of milk proteins has been considered a useful technique for nutritional investigations (Gaudichon et al. 1995; Gaudichon et al. 1999; Mahé et al. 1994). Recently, the use of 15N-labelled milk proteins were used to distinguish exogenous from endogenous N fractions in the human intestine after ingestion of 15N-milk or 15N-yogurt (Gaudichon et al. 1995). These authors pointed out that the jejunal flux of 15N was different for milk and yogurt. It is known that milk proteins and lactose undergo preliminary hydrolysis during lactic fermentation (Tamine & Deeth, 1980). It is also suggested that lactic fermentation enhances the nutritional value of milk proteins (Vass et al. 1984).
17
Brouček, J., P. Kišac, A. Hanus, M. Uhrinčať, and V. Foltys. "Effects of rearing, sire and calving season on growth and milk efficiency in dairy cows." Czech Journal of Animal Science 49, No. 8 (December 13, 2011): 329–39. http://dx.doi.org/10.17221/4317-cjas.
Анотація:
32 primiparous cows were used. We tested the hypotheses that live weight and milk efficiency were influenced by the system of rearing from the second week of life to weaning, by the sire, and by the season of calving. Three groups were created according to a rearing system (A – pens with automatic drinking cups, H – individual huts and N – pens with nursing cows). Primiparous cows originated from four sires and were divided according to the season of calving (spring, summer, autumn and winter). Group N had the highest LW (540.5 kg) and group A the lowest (504.8 kg) in the 1st month of the first lactation. In the 10th month of lactation, the weights were 554.5 kg (A), 566.9 kg (H), and 575.1 kg (N). ADG from the 2nd to 10th month of lactation were statistically different between the groups. Other significances were found by the comparison of animals according to sires (P < 0.05). Group N had the highest milk efficiency almost in all months of lactation. The lowest production was recorded in group A. Daughters of the sire F<sub>3</sub> reached the highest milk production except the seventh and eighth months. Significant differences were calculated in the 5th and in the 6th month between daughters of sires F<sub>3</sub> and F<sub>1</sub>. Dairy cows calving in summer showed the lowest milk yield in the 3rd, 4th, 7th and 9th month. The most productive were cows calving in WIN and in SP. Group N achieved the highest production of milk for 305-day lactation (N 6 894.1 ± 879.8 kg) and animals fed from automatic drinking cups the lowest (A 5 757.5 ± 865.5 kg). A similar trend was recorded also in FCM milk. The content of fat was highest in group A (4.1%) and the lowest in group H (3.57%). Animals of group N produced highly significantly more proteins than group A (215.3 kg versus 180.9 kg, P < 0.01). Group A produced the significantly lowest amount of lactose and nonfat solids over 305 days of lactation. The content of total solids was the highest in group A. Production of total solids was the highest in group N and the lowest in group A (846.5 kg versus 749.8 kg; P < 0.05). The effect of the sire lineage was significantly expressed in milk production and in the content of fat, proteins, lactose, nonfat solids and total solids. The production of milk, proteins, lactose, nonfat solids and content of fat and lactose for 305-day lactation statistically differed according to the season of calving. Dairy cows calving in WIN yielded the highest amount of milk and proteins, cows calving in SP produced the highest amount of lactose and nonfat solids. Dairy cows calving in SU produced the lowest amount of milk, protein, lactose and nonfat solids.
18
Gellrich, K., H. H. D. Meyer, and S. Wiedemann. "Composition of major proteins in cow milk differing in mean protein concentration during the first 155 days of lactation and the influence of season as well as short-term restricted feeding in early and mid-lactation." Czech Journal of Animal Science 59, No. 3 (March 18, 2014): 97–106. http://dx.doi.org/10.17221/7289-cjas.
Анотація:
A variety of proteins contributes greatly to the unique nutritional and functional quality of dairy cow milk. Particularly, milk casein content and composition have substantial influence on the processing capabilities. In the present study, milk of 23 multiparous Holstein-Friesian cows, grouped as high- (3.49 ± 0.05%; n = 11) and low-protein (3.03 ± 0.05%; n = 12) cows, was sampled approximately weekly during the first 155 days of lactation to determine the course of relative milk protein composition (α-lactalbumin; β-lactoglobulin; α-, β-, and κ-casein). Furthermore, feed restrictions by 30% of dry matter intake in early and mid-lactation as well as experimental tissue biopsies were conducted to observe their effect on milk protein composition. Milk protein composition was relatively stable and displayed similar concentration patterns throughout the experimental period between both groups. Mean relative concentrations of α-, β-, κ-casein, α-lactalbumin, and β-lactoglobulin were 34.2, 31.4, 16.0, 2.1, and 9.7% of total protein, respectively. Feed restrictions did not alter milk protein composition, whereas the season influenced α- and β-casein as well as α-lactalbumin. Further, effects were observed in both groups at times of unfamiliar stressful situations caused by taking liver or muscle biopsies. As a result, the relative concentration of β-casein increased. Therefore, acute stress factors may lead to a deviation in milk protein composition and should be avoided.
19
Schaar, Johan, та Hans Funke. "Effect of subclinical mastitis on milk plasminogen and plasmin compared with that on sodium, antitrypsin and N-acetyl-β-D-glucosaminidase". Journal of Dairy Research 53, № 4 (листопад 1986): 515–28. http://dx.doi.org/10.1017/s0022029900033045.
Анотація:
SummaryThe effect of subclinical mastitis on levels of plasminogen and plasmin in milk from cows in a high-yielding herd was investigated. Comparisons were made with levels of milk Na, antitrypsin and N-acetyl-β-D-glucosaminidase (NAGase). In samples from mastitic quarters plasminogen activity, as measured after activation to plasmin, increased by only 21% and plasmin by 82%, while NAGase increased by 307 %. Plasminogen was the only component that was normally distributed, all other components showed more or less skewed distributions. Plasmin and plasminogen were significantly related to the other components. However, plasminogen plateaued when the other components continued to increase. There was thus no further increase in plasminogen with the severity of inflammation as with the other components. Plasmin showed a similar although less pronounced tendency. Results of treatment of mastitic whey samples with acid suggested that the non-linear increase in plasmin activity was due to interaction with acid-labile proteinase inhibitors. Mastitis led to dissociation of plasminogen and plasmin from the casein micelles. The degree of activation of plasminogen was higher with casein-associated than with soluble plasminogen in both healthy and mastitic milks. Plasmin was very closely related to milk Na, which is a sensitive indicator of epithelial integrity. It is suggested that plasmin contributes to Na leakage into milk by degrading membrane proteins of the epithelial lining. Plasminogen and antitrypsin, which are both plasma proteins, were not identically affected by stage of lactation, indicating nonidentical modes of transport from plasma to milk.
20
POL, IRENE E., HENNIE C. MASTWIJK, ROB A. SLUMP, MONA E. POPA, and EDDY J. SMID. "Influence of Food Matrix on Inactivation of Bacillus cereus by Combinations of Nisin, Pulsed Electric Field Treatment, and Carvacrol." Journal of Food Protection 64, no. 7 (July 1, 2001): 1012–18. http://dx.doi.org/10.4315/0362-028x-64.7.1012.
Анотація:
Carvacrol was used as a third preservative factor to enhance further the synergy between nisin and pulsed electric field (PEF) treatment against vegetative cells of Bacillus cereus. When applied simultaneously with nisin (0.04 μg/ml), carvacrol (0.5 mM) enhanced the synergy found between nisin and PEF treatment (16.7 kV/cm, 30 pulses) in potassium-N-2-hydroxy-ethylpiperazine-N-ethanesulfonic acid (HEPES) buffer. The influence of food ingredients on bactericidal activity was tested using skimmed milk that was diluted to 20% with sterile demineralized water. The efficacy of PEF treatment was not affected by the presence of proteins, and results found in HEPES buffer correlated well with results in milk (20%). Nisin showed less activity against B. cereus in milk. Carvacrol was not able to enhance the synergy between nisin and PEF treatment in milk, unless used in high concentrations (1.2 mM). This concentration in itself did not influence the viable count. Carvacrol did act synergistically with PEF treatment in milk, however not in HEPES buffer. This synergy was not influenced by proteins in milk, as 5% milk still allows synergy between carvacrol and PEF treatment to the same extent as 20% milk.
21
Holm, Matilda, Mayank Saraswat, Sakari Joenväärä, Antti Seppo, R. John Looney, Tiialotta Tohmola, Jutta Renkonen, Risto Renkonen, and Kirsi M. Järvinen. "Quantitative glycoproteomics of human milk and association with atopic disease." PLOS ONE 17, no. 5 (May 13, 2022): e0267967. http://dx.doi.org/10.1371/journal.pone.0267967.
Анотація:
The prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence of a protective “farm effect”. The Old Order Mennonite (OOM) community in Upstate New York have traditional, agrarian lifestyles, a low rate of atopic disease, and long periods of exclusive breastfeeding. Human milk proteins are heavily glycosylated, although there is a paucity of studies investigating the milk glycoproteome. In this study, we have used quantitative glycoproteomics to compare the N-glycoprotein profiles of 54 milk samples from Rochester urban/suburban and OOM mothers, two populations with different lifestyles, exposures, and risk of atopic disease. We also compared N-glycoprotein profiles according to the presence or absence of atopic disease in the mothers and, separately, the children. We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. These four glycopeptides may be associated with the development of atopic disease. The findings of this study suggest that the differential glycosylation of milk proteins could be linked to atopic disease.
22
Cao, Mingxing, Lin Huang, Suyu Jin, Mengbo Zhao, and Yucai Zheng. "Comparative Proteomics Study of Yak Milk from Standard and Naturally Extended Lactation Using iTRAQ Technique." Animals 12, no. 3 (February 7, 2022): 391. http://dx.doi.org/10.3390/ani12030391.
Анотація:
Extended lactation is a common phenomenon in lactating yaks under grazing and natural reproduction conditions. To elucidate differences in milk protein compositions and mammary gland functions between yaks of standard lactation (TL yaks) and prolonged lactation (HL yaks), whole milk samples of TL yaks and HL yaks (n = 15 each) were collected from a yak pasture at the northwest highland of China. The iTRAQ technique was used to compare the skim milk proteins in the two yak groups. A total of 202 differentially expressed proteins (DEPs) were revealed, among which 109 proteins were up-regulated and 93 were down-regulated in the milk of HL yaks compared to TL yaks. Caseins including κ-casein, αs1-casein, αs2-casein, and β-casein were up-regulated in HL yak milk over 1.43-fold. The GO function annotation analysis showed that HL yaks produced milk with characteristics of milk at the degeneration stage, similar to that of dairy cows. KEGG enrichment showed that the metabolic pathways with the most differences are those that involve carbohydrate metabolism and the biosynthesis of amino acids. The present results highlight detailed differences in skim milk proteins produced by HL yaks and TL yaks and suggest that the mammary gland of HL yak is at the degeneration stage.
23
Wang, Chaoyue, Leonardo Susta, and Shai Barbut. "Restoring Functionalities in Chicken Breast Fillets with Spaghetti Meat Myopathy by Using Dairy Proteins Gels." Gels 8, no. 9 (September 2, 2022): 558. http://dx.doi.org/10.3390/gels8090558.
Анотація:
The use of caseinate, whole milk powder, and two whey protein preparations (WP; 2% w/w) was studied in minced meat made with normal breast (NB), and ones showing spaghetti meat (SM). SM is an emerging myopathy known for muscle fiber separation and lower protein content, costing $100s of millions to the industry. Using SM without dairy proteins resulted in a higher cooking loss (SM: 3.75%, NB: 2.29%; p < 0.05), and lower hardness (SM: 29.83 N, NB: 34.98 N), and chewiness (SM: 1.29, NB: 1.56) compared to NB. Using dairy proteins, except WP concentrate and WP isolate, significantly improved yield and increased hardness. Adding WP isolate to SM resulted in a similar texture profile as NB samples without dairy proteins (34 and 35 N hardness; 0.22 and 0.24 springiness; 1.57 and 1.59 chewiness values, respectively). Adding caseinate and whole milk to SM showed a more substantial effect of improving water-holding capacity, increasing hardness, gumminess, and chewiness compared to adding WP; i.e., adding caseinate and milk powder resulted in higher values for those parameters compared to NB without additives. Overall, it is shown that dairy proteins can be added to SM to produce minced poultry meat products with similar or higher yield and texture profiles compared to using normal breast fillets.
24
KHORASANI, G. R., W. C. SAUER, F. MAENHOUT, and J. J. KENNELLY. "SUBSTITUTION OF MILK PROTEIN WITH SOYFLOUR OR MEAT-SOLUBLES N CALF MILK REPLACERS." Canadian Journal of Animal Science 69, no. 2 (June 1, 1989): 373–82. http://dx.doi.org/10.4141/cjas89-042.
Анотація:
Five Holstein male calves were fitted with ileo-cecal re-entrant cannulae. Calves were fed five milk replacers according to a 5 × 5 Latin square design. The control milk replacer (SM-100) was formulated so that 80% of the crude protein originated from skim milk powder and the remainder from whey proteins. In the test milk replacers, 40 or 60% of the skim milk protein was replaced by protein from soyflour (SF-40; SF-60) or meat-solubles (MS-40; MS-60). Diets were supplied at a rate of 50 g dry matter per unit metabolic body size (BWkg0.75) d−1. Average daily gain (ADG) and protein efficiency ratio (PER) decreased when SM was replaced by SF or MS. This depression was greater (P < 0.05) with higher levels of substitution and more marked for MS than SF. Plasma methionine concentration was lowest in calves fed the SF-60 and MS-60 diets. Average ileal digestibilities of the total of the indispensable amino acids were highest in SM-100 (94.6%), intermediate in MS-40 (88.0%) and MS-60 (86.8%) and lowest in SF-40 (83.8%) and SF-60 (82.1%). Fecal amino acid digestibilities did not differ between diets (P > 0.05). In summary, there was a decrease in ADG and PER when milk protein was replaced by protein from SF or MS. The inferior performance of calves fed SF- or MS-containing milk replacers is, in part, related to a lower digestible amino acid supply as measured in digesta collected from the distal ileum. Key words: Calf, milk replacers, soyflour, meat-solubles, amino acid digestibility
25
Lai, Giacomo, Pierluigi Caboni, Cristina Piras, Massimo Pes, Maria Sitzia, Margherita Addis, Antonio Pirisi, and Paola Scano. "Development and Chemico-Physical Characterization of Ovine Milk-Based Ingredients for Infant Formulae." Applied Sciences 13, no. 1 (January 3, 2023): 653. http://dx.doi.org/10.3390/app13010653.
Анотація:
The great majority of infant formula (FM) for neonate’s nutrition are produced using ingredients from cow milk. Recently, some countries, such as China and New Zealand, are turning their attention to the use of ovine milk ingredients for FM production. In this study, a pilot plant process has been set up to produce infant formula ingredients from Sarda sheep milk. To meet the nutritional needs of neonates (0–6 and 6–12 months of age) two different liquid milk-derived formulations (IF1 and IF2, respectively) obtained mixing whole milk, skimmed milk, and whey milk ultrafiltration concentrate (retentate) were produced. Compositional analysis of milk, retentate, and the final IFs showed that the two formulations contain elements of nutritional interest, such as well-balanced content of high biological value proteins (casein:whey proteins ratio of 30:70 and 60:40 for IF1 and IF2, respectively), vitamin A, E and B5, cholesterol, minerals, nucleotides, free amino acids and essential fatty acids (n–6:n–3 ~1), compatible with the growth and development needs of neonates. Therefore, the obtained IF1 and IF2 can be proposed as valuable ovine dairy ingredients for FM manufacturing. Further studies will be necessary to verify the adaptability of the developed process from laboratory to industrial scale application.
26
Wiles, Peter G., Ian K. Gray, Roger C. Kissling, C. Delahanty, J. Evers, K. Greenwood, K. Grimshaw, et al. "Routine Analysis of Proteins by Kjeldahl and Dumas Methods: Review and Interlaboratory Study Using Dairy Products." Journal of AOAC INTERNATIONAL 81, no. 3 (May 1, 1998): 620–32. http://dx.doi.org/10.1093/jaoac/81.3.620.
Анотація:
abstract The Kjeldahl and Dumas (combustion) methods were compared in 11 laboratories analyzing samples of milk, skim milk powder, whole milk powder, whey protein concentrate, infant formula, casein, caseinate, 2 reference compounds (glycine and EDTA), and a secondary reference skim milk powder. The comparison was conducted by using international standards where applicable. Overall means were 8.818 g N/100 g by the Kjeldahl method and 8.810 g N/100 g by the Dumas method. No evidence was found for a consistent bias between methods that may be of concern in the trading of dairy produce. A review of more than 10 related trials revealed a lack of consensus in the bias between the 2 methods, suggesting that differences in methodology and sources of systematic error may be contributors. For samples containing >2 g N/100 g, the Dumas relative repeatability and reproducibility standard deviations were consistently about 0.35 and 0.75%, respectively, whereas the corresponding Kjeldahl values declined generally with N content and were significantly larger. The Dumas precision characteristics may be due to the dominance of Leco analyzers in this trials, and in most other recent trials, rather than an inherent method attribute. Protein determination methods for dairy products need to be reviewed and updated. The Dumas method needs Codex Alimentarius status as a recognized test method.
27
Gaudichon, Clarie, Sylvain Mahé, Nils Roos, Robert Benamouzig, Catherine Luengo, Jean-François Huneau, Hinrich Sick, Christine Bouley, Jaques Rautureau, and Daniel Tome. "Exogenous and endogenous nitrogen flow rates and level of protein hydrolysis in the human jejunum after [15N]milk and [15N]yoghurt ingestion." British Journal of Nutrition 74, no. 2 (August 1995): 251–60. http://dx.doi.org/10.1079/bjn19950128.
Анотація:
Milk and yoghurt proteins were 15N-labelled in order to measure the flow rate of exogenous N during digestion in the human intestine. After fasting overnight, sixteen healthy volunteers, each with a naso-jejunal tube, ingested either [15N]milk (n 7) or [15N]yoghurt (n 9). Jejunal samples were collected every 20 min for 4 h. A significant stimulation of endogenous N secretion was observed during the 20–60 min period after yoghurt ingestion and the 20–40 min period after milk ingestion. The endogenous N flows over a 4 h period did not differ between the groups (44·3 (SEM 6·5) mmol for milk and 63·5 (SEM 5·9) mmol for yoghurt). The flow rates of exogenous N indicated a delayed gastric emptying of the yoghurt N compared with N from milk. The jejunal non-protein N (NPN) flow rate increased significantly after milk and yoghurt ingestion due to an increase in the exogenous NPN flow rate. The NPN fraction of exogenous N ranged between 40 and 80%. The net gastro-jejunal absorption of exogenous N did not differ significantly between milk (56·7 (SEM 8·5)%) and yoghurt (50·9 (SEM 7)%). The high level of exogenous N hydrolysis is in accordance with the good digestibility of milk products. Fermentation modifies only the gastric emptying rate of N and does not affect the level of diet hydrolysis, the endogenous N stimulation or the digestibility rate.
28
Ali, Aarif, Muneeb U. Rehman, Saima Mushtaq, Sheikh Bilal Ahmad, Altaf Khan, Anik Karan, Amir Bashir Wani, Showkat Ahmad Ganie, and Manzoor Ur Rahman Mir. "Biochemical and Computational Assessment of Acute Phase Proteins in Dairy Cows Affected with Subclinical Mastitis." Current Issues in Molecular Biology 45, no. 7 (June 26, 2023): 5317–46. http://dx.doi.org/10.3390/cimb45070338.
Анотація:
Subclinical mastitis (SCM) is a predominant form of mastitis wherein major visible signs of disease are absent. The present study aimed to determine acute phase proteins (APPs) like ferritin, C-reactive protein (CRP), and microalbumin (Malb) in 135 composite milk and serum samples of healthy (n = 25) and SCM (n = 110) cows. As bovine mastitis is an inflammatory disease, the present study also aimed at finding novel anti-inflammatory compounds from natural sources by repurposing approach using computational studies. The findings of the present study revealed substantial elevation (p < 0.001) in milk SCC and an increase in ferritin, CRP, and Malb (p < 0.001) in milk and sera of the SCM group as compared to healthy animals. Receiver operating characteristics of milk SCC, milk, and serum APPs unraveled statistically substantial alteration (p < 0.001). Further, SCC was correlated with milk APPs ferritin (r = 0.26 **, p < 0.002), CRP (r = 0.19 *, p < 0.02), and Malb (r = 0.21 *, p < 0.01). Additionally, milk SCC was correlated with serum ferritin (r = 0.28 **, p < 0.001), CRP (r = 0.16, p > 0.05), and Malb (r = 0.16, p > 0.05). The findings of molecular docking revealed that Chaetoglobosin U was the most effective molecule that showed the highest binding affinity (kcal/mol) of −10.1 and −8.5 against ferritin and albumin. The present study concluded that the estimation of cow-side tests, SCC, and APPs in milk/serum is suitable to detect SCM and screening herd community. Furthermore, Chaetoglobosin U could be developed as a promising anti-inflammatory inhibitor; however, further studies are required to validate these findings.
29
Prudden, Anthony R., Lin Liu, Chantelle J. Capicciotti, Margreet A. Wolfert, Shuo Wang, Zhongwei Gao, Lu Meng, Kelley W. Moremen, and Geert-Jan Boons. "Synthesis of asymmetrical multiantennary human milk oligosaccharides." Proceedings of the National Academy of Sciences 114, no. 27 (June 19, 2017): 6954–59. http://dx.doi.org/10.1073/pnas.1701785114.
Анотація:
Despite mammalian glycans typically having highly complex asymmetrical multiantennary architectures, chemical and chemoenzymatic synthesis has almost exclusively focused on the preparation of simpler symmetrical structures. This deficiency hampers investigations into the biology of glycan-binding proteins, which in turn complicates the biomedical use of this class of biomolecules. Herein, we describe an enzymatic strategy, using a limited number of human glycosyltransferases, to access a collection of 60 asymmetric, multiantennary human milk oligosaccharides (HMOs), which were used to develop a glycan microarray. Probing the array with several glycan-binding proteins uncovered that not only terminal glycoepitopes but also complex architectures of glycans can influence binding selectivity in unanticipated manners. N- and O-linked glycans express structural elements of HMOs, and thus, the reported synthetic principles will find broad applicability.
30
Grumach, Anete S., Solange E. I. Jerônimo, Marcia Hage, and Magda M. S. Carneiro-Sampaio. "Nutritional factors in milk from Brazilian mothers delivering small for gestational age neonates." Revista de Saúde Pública 27, no. 6 (December 1993): 455–62. http://dx.doi.org/10.1590/s0034-89101993000600008.
Анотація:
The composition of breast milk from brazilian mothers delivering low birthweight infants and its adequacy as a source of nutrients for this group has not yet been fully elucidated. A total of 209 milk samples from 66 women were analysed. The mothers were divided into three groups: G1, mothers delivering term babies of low birthweight (TSGA, n=16); G2, mothers delivering preterm babies of appropriate birthweight (PTAGA, n=20); G3, mothers delivering term babies of appropriate birthweight (TAGA, n=30). The following factors were analysed: osmolarity, total proteins and protein fractions, creamatocrit, sodium, potassium, calcium and magnesium. Milk samples were collected 48 h and 7, 15, 30 and 60 days after delivery. The groups did not differ significantly in terms of osmolarity, total proteins and fractions, creamatocrit, calcium, magnesium or potassium throughout the study period. Sodium levels were higher in all samples from mothers of TSGA infants and in samples from mothers of PTAGA infants on the 7th, 15th and 30th days than in milk from the TAGA group. The authors consider the needs of the low birthweight and TAGA infants and that these high sodium levels may be necessary for growth of low birthweight infants.
31
Aslebagh, Roshanak, Danielle Whitham, Devika Channaveerappa, Panashe Mutsengi, Brian T. Pentecost, Kathleen F. Arcaro, and Costel C. Darie. "Mass Spectrometry-Based Proteomics of Human Milk to Identify Differentially Expressed Proteins in Women with Breast Cancer versus Controls." Proteomes 10, no. 4 (October 28, 2022): 36. http://dx.doi.org/10.3390/proteomes10040036.
Анотація:
It is thought that accurate risk assessment and early diagnosis of breast cancer (BC) can help reduce cancer-related mortality. Proteomics analysis of breast milk may provide biomarkers of risk and occult disease. Our group works on the analysis of human milk samples from women with BC and controls to investigate alterations in protein patterns of milk that could be related to BC. In the current study, we used mass spectrometry (MS)-based proteomics analysis of 12 milk samples from donors with BC and matched controls. Specifically, we used one-dimensional (1D)-polyacrylamide gel electrophoresis (PAGE) coupled with nanoliquid chromatography tandem MS (nanoLC-MS/MS), followed by bioinformatics analysis. We confirmed the dysregulation of several proteins identified previously in a different set of milk samples. We also identified additional dysregulations in milk proteins shown to play a role in cancer development, such as Lactadherin isoform A, O-linked N-acetylglucosamine (GlcNAc) transferase, galactosyltransferase, recoverin, perilipin-3 isoform 1, histone-lysine methyltransferase, or clathrin heavy chain. Our results expand our current understanding of using milk as a biological fluid for identification of BC-related dysregulated proteins. Overall, our results also indicate that milk has the potential to be used for BC biomarker discovery, early detection and risk assessment in young, reproductively active women.
32
HERMANSEN, JOHN E., STEEN OSTERSEN, NIELS C. JUSTESEN, and OLE AAES. "Effects of dietary protein supply on caseins, whey proteins, proteolysis and renneting properties in milk from cows grazing clover or N fertilized grass." Journal of Dairy Research 66, no. 2 (May 1999): 193–205. http://dx.doi.org/10.1017/s0022029999003477.
Анотація:
The objective of this work was to examine whether variation in the amino acid supply to cows could be a reason for the reduced casein content and poorer renneting properties of milk that often occur in late summer, or whether these effects are related to proteolysis in the raw milk. In a 2×2×2 factorial design, we investigated the effects of sward (clover v. rye-grass) and supplementary feed with a high or low level of rumen-soluble N or of rumen undegradable protein on milk protein composition during the grazing season. A total of 32 Danish Holstein cows were included in the experiment. Milk protein and casein contents and the ratios casein N[ratio ]total N and casein[ratio ]true protein were at a minimum in late summer, whereas the contents of urea, non-protein N and whey protein were higher during this period. These seasonal effects were unrelated to either the type of supplementary feed or the type of sward; neither were they clearly related to proteolysis, although casein[ratio ]true protein was related to the proteose peptone content. The results indicated that whey proteins other than α-lactalbumin or β-lactoglobulin accounted for the higher proportion or concentration of whey protein in late summer. Based on a principal component analysis including variables such as citric acid, lactose and non-protein N, we suggest that the cows' energy supply during this period may be a critical factor in determining the milk protein composition, although our results were not conclusive. There was an interaction between the supplement of rumen undegradable protein and type of sward. When clover was grazed, a high supplement increased the concentrations of protein and casein in milk and the κ-casein[ratio ]total casein ratio. When rye-grass was grazed, the opposite response was found, and overall milk protein yield was not affected. The very low N content of clover in early summer reduced milk protein and casein protein during this period.
33
Ruska, Diana, and Daina Jonkus. "Crude Protein and Non-protein Nitrogen Content in Dairy Cow Milk." Proceedings of the Latvia University of Agriculture 32, no. 1 (December 1, 2014): 36–40. http://dx.doi.org/10.2478/plua-2014-0011.
Анотація:
Abstract Milk composition is of prime economic importance for farmers. Milk total proteins are composed of casein, whey proteins and non-protein nitrogen. The objective of this work was to establish milk crude protein, non-protein nitrogen (NPN) and urea content in dairy cow milk produced in different farms in Latvia. Cow milk samples (n=30) were collected in September 2012 from four different farms breeding diverse cow breeds. Average crude protein, casein and urea content in milk varied significantly among farms. NPN content in cow milk varies among farms - from 0.194% to 0.232%. Average crude protein and casein content was significantly higher (p<0.05) for Latvia Brown breed cows, while NPN content did not differ significantly among breeds. Regression between NPN and urea content in milk was R² = 0.458. Correlation between NPN and urea content was significant (r = 0.677). This study allowed establishing that crude protein and NPN content in milk varied significantly (p<0.05) in farms with differing dairy cow housing and feeding technologies
34
Murphy, Eoin G., Nicolas E. Regost, Yrjö H. Roos, and Mark A. Fenelon. "Powder and Reconstituted Properties of Commercial Infant and Follow-On Formulas." Foods 9, no. 1 (January 13, 2020): 84. http://dx.doi.org/10.3390/foods9010084.
Анотація:
The physical properties of 15 commercially available infant formulas (IF) and follow-on (FO) formulas were analysed. Powders made with intact milk proteins were classified into two groups; Type I—homogenous mixtures of milk powder particles (n = 6); and Type II—heterogeneous mixtures of milk powder particles and tomahawk-shaped α-lactose monohydrate crystals (n = 6). Powders made using hydrolysed proteins were classified as Type III powders (n = 3). Type II powders exhibited similar flow characteristics to Type I powders despite having significantly (p < 0.05) smaller particle size, lower circularity, and greater elongation. Type III powders exhibited lowest particles size, highest surface free fat, and poorest flow properties (p < 0.05 for all). Upon reconstitution of powders (12.5% w/w), no significant difference (p < 0.05) in apparent viscosity was observed between Type I and II powders. Reconstituted Type III powders had relatively poor stability to separation compared to Type I and II powders, caused by large starch granules and/or poor emulsification by hydrolysed proteins. Overall, this study illustrated the range of physical behaviour and structures present in commercial IF powders. In particular, the effect of dry addition of lactose and the hydrolysis of protein were found to have major effects on physical properties.
35
Adkins, Yuriko, Steven C. Zicker, Allan Lepine, and Bo Lönnerdal. "Changes in nutrient and protein composition of cat milk during lactation." American Journal of Veterinary Research 58, no. 4 (April 1, 1997): 370–75. http://dx.doi.org/10.2460/ajvr.1997.58.04.370.
Анотація:
Abstract Objective To evaluate changes in the nutrient and protein composition of cat milk during lactation. Animals 12 lactating domestic shorthair cats. Procedure Milk samples collected on days 1, 3, 7, 14, 28, and 42 after parturition were analyzed for concentrations of nitrogen, nonprotein nitrogen, casein, whey proteins, amino acids, total lipids, lactose, citrate, minerals, and trace elements. Individual milk proteins (caseins and whey proteins) were analyzed by use of polyacrylamide gradient gel electrophoresis. Results True protein concentration ranged from 6.3 to 8.6% and was as high in mature milk as in colostrum. Nonprotein nitrogen as a portion of total N was constant (approx 8%), as was the whey-to-casein ratio (approx 50:50). Total lipid concentration was high (9.3%) in colostrum, rapidly decreased, then increased to 9% in mature milk. Lactose concentration was constant at 4%. Milk calcium, iron, and copper concentrations increased markedly during lactation, and magnesium and zinc values remained constant. Colostrum and early milk had a low Ca-to-P ratio of 0.4:0.9. Although calcium concentration increased with time, phosphate concentration also increased so that the Ca-to-P ratio remained constant in mature milk at 1.0: 1.2. The major whey proteins had molecular weights of approximately 14,000, 19,000, 40,000 and 80,000. The 80,000 protein (possibly lactoferrin) decreased in concentration during lactation. Two major casein subunits of approximately 28,000 and 33,000 were found, and both increased during early lactation. Conclusions Nutrient composition of cat milk and, thus, provision of nutrients to nursing kittens changes over time. (Am J Vet Res 1997;58:370-375)
36
Breier, Bernhard H., Stella R. Milsom, Werner F. Blum, Jürg Schwander, Brian W. Gallaher, and Peter D. Gluckman. "Insulin-like growth factors and their binding proteins in plasma and milk after growth hormone-stimulated galactopoiesis in normally lactating women." Acta Endocrinologica 129, no. 5 (November 1993): 427–35. http://dx.doi.org/10.1530/acta.0.1290427.
Анотація:
We performed a double-blind randomized placebo-controlled trial of recombinant human growth hormone (hGH) in normally lactating women (N = 8 per group) to investgate the endocrine mode of action of the galactopoietic effect of this hormone. Insulin-like growth factors I (IGF-I) and II (IGF-II) and their binding proteins (IGFBP-1, IGFBP-2 and IGFBP-3) were measured by radioimmunoassay in plasma and milk samples collected throughout the study. All assays were validated for human plasma and milk. Human GH treatment (0.1 IU·kg−1 body wt·day−1 for 7 days) increased plasma concentrations of IGF-I from 22.1±1.3 nmol/l (mean±sem) to 59.7±2.5 nmol/l (p<0.01). At the end of the study the increase in plasma IGF-I correlated significantly with the increase in milk volume (r=0.67, p<0.005, N=16). The IGF-I levels were considerably lower in milk, with 0.14±0.03 nmol/l before and 0.31±0.04 nmol/l after hGH treatment. The increase in milk IGF-I levels (134.0±14.5%) with hGH treatment was significant (p<0.01) and plasma and milk IGF-I concentrations correlated significantly when considering all samples of the study (r=0.45, p<0.001, N= 56). The concentrations of IGF-II were not changed significantly with hGH treatment in plasma (52.5±2.5 nmol/l before and 42.6±3.9 nmol/l after treatment) or milk (2.1±0.29 nmol/l before and 2.3±0.49 nmol/l after hGH treatment). The IGFBP-1 levels were not changed with hGH treatment in plasma (approximately 1.3 nmol/l) or milk (approximately 0.2 nmol/l). Although IGFBP-2 concentrations in plasma were reduced significantly (p<0.05) after hGH treatment (11.1±1.5 before and 8.4±0.9 nmol/l after hGH treatment), milk IGFBP-2 levels did not respond to hGH treatment. Milk levels were markedly higher (sevenfold) in comparison to plasma levels. Plasma IGFBP-3 showed a delayed and smaller rise with hGH treatment in comparison to the rise observed in IGF-I. However, at the end of the study the response (38.6±4.9%) to hGH was significant (p<0.01) and a significant correlation was observed also between the increase in IGFBP-3 and the increase in milk volume (r=0.55, p =0.03, N=16). Plasma IGF-I and IGFBP-3 concentrations correlated significantly when considering all samples of the study (r=0.61, p<0.001, N=63). Milk IGFBP-3 levels were approximately 100-fold lower in comparison to plasma levels and did not correlate with any other measurements. Our data show that hGH-stimulated galactopoiesis in normally lactating women is mediated by significant elevations of plasma and milk IGF-I and plasma IGFBP-3. While IGF-I may be a principal mediator of the galactopoietic effect of hGH, we cannot simply attribute the action of hGH solely to a systemic rise in IGF-I. The increase in plasma IGFBP-3 with hGH treatment suggests that IGFBP-3 could facilitate the delivery of IGF-I to the mammary gland. The high concentrations of IGFBP-2 in milk suggest that mammary epithelial IGFBP-2 may direct regional tissue distribution of IGF-I to the site of milk synthesis.
37
Åkerstedt, Maria, Karin Persson Waller, and Åse Sternesjö. "Haptoglobin and serum amyloid A in bulk tank milk in relation to raw milk quality." Journal of Dairy Research 76, no. 4 (September 17, 2009): 483–89. http://dx.doi.org/10.1017/s0022029909990185.
Анотація:
The aim of the present study was to evaluate relationships between the presence of the two major bovine acute phase proteins haptoglobin (Hp) and serum amyloid A (SAA) and raw milk quality parameters in bulk tank milk samples. Hp and SAA have been suggested as specific markers of mastitis but recently also as markers for raw milk quality. Since mastitis has detrimental effects on milk quality, it is important to investigate whether the presence of Hp or SAA indicates such changes in the composition and properties of the milk. Bulk tank milk samples (n=91) were analysed for Hp, SAA, total protein, casein, whey protein, proteolysis, fat, lactose, somatic cell count and coagulating properties. Samples with detectable levels of Hp had lower casein content, casein number and lactose content, but higher proteolysis than samples without Hp. Samples with detectable levels of SAA had lower casein number and lactose content, but higher whey protein content than samples without SAA. The presence of acute phase proteins in bulk tank milk is suggested as an indicator for unfavourable changes in the milk composition, e.g. protein quality, due to udder health disturbances, with economical implications for the dairy industry.
38
Wang, Jing, Rui Liu, Xiaoyang Huang, Yuexin Bao, Xiaohong Wang, Huaxi Yi, and Youyou Lu. "The Effect of Nanoscale Modification of Nisin by Different Milk-Derived Proteins on Its Physicochemical Properties and Antibacterial Activity." Foods 13, no. 11 (May 22, 2024): 1606. http://dx.doi.org/10.3390/foods13111606.
Анотація:
Nisin is used as a natural food preservative because of its broad-spectrum antimicrobial activity against Gram-positive bacteria. However, free nisin is susceptible to various factors that reduce its antimicrobial activity. Milk protein, a protein derived from milk, has self-assembly properties and is a good carrier of bioactive substances. In this study, lactoferrin–nisin nanoparticles (L-N), bovine serum albumin–nisin nanoparticles (B-N), and casein–nisin nanoparticles (C-N) were successfully prepared by a self-assembly technique, and then their properties were investigated. The studies revealed that lactoferrin (LF) and nisin formed L-N mainly through hydrophobic interactions and hydrogen bonding, and L-N had the best performance. The small particle size (29.83 ± 2.42 nm), dense reticular structure, and good thermal stability, storage stability, and emulsification of L-N laid a certain foundation for its application in food. Further bacteriostatic studies showed that L-N enhanced the bacteriostatic activity of nisin, with prominent inhibitory properties against Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus, which mainly disrupted the cell membrane of the bacteria. The above results broaden our understanding of milk protein–nisin nanoparticles, while the excellent antibacterial activity of L-N makes it promising for application as a novel food preservative, which will help to improve the bioavailability of nisin in food systems.
39
Yamaguchi, Toshiyuki, Hirofumi Fukudome, Junichi Higuchi, Tomoki Takahashi, Yuta Tsujimori, Hiroshi M. Ueno, Yasuhiro Toba, and Fumihiko Sakai. "Label-Free Liquid Chromatography–Mass Spectrometry Quantitation of Relative N- and O-Glycan Concentrations in Human Milk in Japan." International Journal of Molecular Sciences 25, no. 3 (February 1, 2024): 1772. http://dx.doi.org/10.3390/ijms25031772.
Анотація:
Human milk is abundant in carbohydrates and includes human milk oligosaccharides (HMOs) and N/O-glycans conjugated to proteins. HMO compositions and concentrations vary in individuals according to the maternal secretor status based on the fucosyltransferase 2 genotype; however, the profile of N/O-glycans remains uninvestigated because of the analytical complexity. Herein, we applied a label-free chromatography–mass spectrometry (LC–MS) technique to elucidate the variation in the composition and concentration of N/O-glycans in human milk. We used label-free LC–MS to relatively quantify 16 N-glycans and 12 O-glycans in 200 samples of Japanese human milk (1–2 months postpartum) and applied high performance anion exchange chromatography with pulsed amperometric detection to absolutely quantify the concentrations of 11 representative HMOs. Cluster analysis of the quantitative data revealed that O-glycans and several HMOs were classified according to the presence or absence of fucose linked to galactose while N-glycans were classified into a different group from O-glycans and HMOs. O-glycans and HMOs with fucose linked to galactose were more abundant in human milk from secretor mothers than from nonsecretor mothers. Thus, secretor status influenced the composition and concentration of HMOs and O-glycans but not those of N-glycans in human milk.
40
Meredith-Dennis, Laura, Gege Xu, Elisha Goonatilleke, Carlito B. Lebrilla, Mark A. Underwood, and Jennifer T. Smilowitz. "Composition and Variation of Macronutrients, Immune Proteins, and Human Milk Oligosaccharides in Human Milk From Nonprofit and Commercial Milk Banks." Journal of Human Lactation 34, no. 1 (June 14, 2017): 120–29. http://dx.doi.org/10.1177/0890334417710635.
Анотація:
Background: When human milk is unavailable, banked milk is recommended for feeding premature infants. Milk banks use processes to eliminate pathogens; however, variability among methods exists. Research aim: The aim of this study was to compare the macronutrient (protein, carbohydrate, fat, energy), immune-protective protein, and human milk oligosaccharide (HMO) content of human milk from three independent milk banks that use pasteurization (Holder vs. vat techniques) or retort sterilization. Methods: Randomly acquired human milk samples from three different milk banks ( n = 3 from each bank) were analyzed for macronutrient concentrations using a Fourier transform mid-infrared spectroscopy human milk analyzer. The concentrations of IgA, IgM, IgG, lactoferrin, lysozyme, α-lactalbumin, α antitrypsin, casein, and HMO were analyzed by mass spectrometry. Results: The concentrations of protein and fat were significantly ( p < .05) less in the retort sterilized compared with the Holder and vat pasteurized samples, respectively. The concentrations of all immune-modulating proteins were significantly ( p < .05) less in the retort sterilized samples compared with vat and/or Holder pasteurized samples. The total HMO concentration and HMOs containing fucose, sialic acid, and nonfucosylated neutral sugars were significantly ( p < .05) less in retort sterilized compared with Holder pasteurized samples. Conclusion: Random milk samples that had undergone retort sterilization had significantly less immune-protective proteins and total and specific HMOs compared with samples that had undergone Holder and vat pasteurization. These data suggest that further analysis of the effect of retort sterilization on human milk components is needed prior to widespread adoption of this process.
41
Boza, Julio J., Olga Martínez-Augustin, Luis Baró, M. Dolores Suarez, and Angel Gil. "Protein v. enzymic protein hydrolysates. Nitrogen utilization in starved rats." British Journal of Nutrition 73, no. 1 (January 1995): 65–71. http://dx.doi.org/10.1079/bjn19950009.
Анотація:
The present study was carried out to compare the effects of four isoenergetic and isonitrogenous diets on the N utilization, total serum protein concentration and serum amino acid profile in starved rats at weaning. These diets differed only in the molecular form of two milk proteins (whey protein and casein), which were either native or partly hydrolysed. Male Wistar rats at weaning were fasted for 3 d and then refed with one of the four diets for 48 h. No differences were observed in the body weight gain, protein digestibility and total serum protein concentration between groups after the refeeding period and all the N balances were positive. N retention was higher in the two groups of rats given the protein-hydrolysate-based diets compared with those given the intact-protein-based diets. This was associated with a lower urinary N excretion in rats, given the whey-protein-hydrolysate and the casein-hydrolysate diets. Despite this fact, the serum amino acid pattern of rats given the hydrolysed protein diet was very similar to that of those given the corresponding native protein diet. In conclusion, we have proved that enzymic hydrolysates from milk proteins have equivalent effects to native proteins in recovery after starvation in rats at weaning, on N absorption, total serum protein concentration and serum amino acid profile, and even give a higher N retention. We did not observe any harmful effect in using protein hydrolysates instead of native proteins.
42
Singh, Samridhi, Ratan K. Choudhary, and Navdeep Singh. "Comparative Protein Profiling of Blood and Milk of Early Pregnant Buffaloes Using SDS-PAGE." Animal Reproduction Update 4, no. 1 (2024): 11–16. http://dx.doi.org/10.48165/aru.2023.4.1.3.
Анотація:
Conventional methods for detecting pregnancy in buffalo are inefficient before 30-days after artificial insemination. A proteomic approach for high-resolution analysis of blood plasma and milk proteins by SDS polyacrylamide gel electrophoresis (PAGE) is of great importance. Blood and milk samples were collected on days 0 (before insemination) and 20 and 25 after insemination from retrospective pregnant (n = 6) and non-pregnant (n = 6) animals. An equal volume of samples was loaded in (12%-4%) SDS gel to resolve major plasma and milk proteins. Plasma proteins showed three distinct zones – high, medium, and low molecular weight (MW). In the high MW zone (135 - 250 kDa), ~208 kDa and ~190 kDa proteins were visible. In the moderate MW zone (60 -75 kDa), 5-6 proteins like albumin (~70 kDa), 75 kDa, and 63 kDa consisting of pregnancy-associated glycoproteins, ~73 kDa being dominant. In the low MW zone (25 – 35 kDa), a clear band of 27 kDa was visible. Proteins in milk supernatants showed four distinct zones of proteins – very high, high, medium, and low molecular weight (MW) zones. In the very high MW zone (135 - 250 kDa), ~208 kDa and ~190 kDa proteins were visible. In the high MW zone (60 -75 kDa), proteins like lactoferrin (~78.2 kDa), 73 kDa, albumen (~66 kDa), and a heavy chain of immunoglobulin (IgG) (~54 kDa) were detected. In the medium MW zone (25 – 35 kDa), clear bands of milk caseins, namely thick alpha S2 casein (29 kDa), and other caseins. In the low MW zone (11 – 20 kDa), two thick bands of 18 kDa and 12 kDa were visible. The mean gray values (sum of the gray values of all the pixels in the peak divided by the number of pixels) of the 73 kDa protein peak were analyzed in two-way ANOVA using GraphPad (ver 8.0). Repeated measures of ANOVA were selected where pregnancy status (pregnant and non-pregnant) defined column factor and time (0-, 20- and 25-day) defined row factor. The expression of 73 kDa protein in blood plasma was higher (P = 0.04) in pregnant women than in non-pregnant buffaloes at 20- and 25-days after insemination. However, in milk, there was no difference in the expression of the 73 kDa protein between pregnant and non-pregnant buffaloes across time. Variation in animals within the groups was significantly high. The differential expression of ~73 kDa protein in plasma and precise identification of the protein can be a diagnostic marker for early pregnancy detection in buffaloes.
43
Muths, E. "Milk Composition in a Field Population of Red Kangaroos, Macropus Rufus (Desmarest) (Macropodidae: Marsupialia)." Australian Journal of Zoology 44, no. 2 (1996): 165. http://dx.doi.org/10.1071/zo9960165.
Анотація:
The composition of milk from early pouch life (0-40 days) to weaning (360 days) was determined in samples collected from a field population of red kangaroos, Macropus rufus (n = 150). Total milk solids increased from 11% at 0-40 days to 26% at permanent emergence from the pouch (235 days), then decreased towards weaning. Compared with other macropodids, milk from red kangaroos is relatively dilute. Carbohydrate concentrations increased from 2.0 to 6.2% at about Day 235 then declined while lipid concentrations increased from 3.9 to 10.3% over the course of lactation. Protein values increased from 5.0 to 7.0% prior to pouch emergence. Whey proteins were separated by means of SDS PAGE, identifying and confirming the presence of several phase-specific proteins. These results are similar to those reported for components of milk in captive red kangaroos and therefore confirm the general macropodid pattern of changing milk composition throughout lactation for a field population of red kangaroos.
44
Rosa, Fernanda, Boris L. Zybailov, Galina V. Glazko, Yasir Rahmatallah, Stephanie Byrum, Samuel G. Mackintosh, Anne K. Bowlin, and Laxmi Yeruva. "Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model." Nutrients 13, no. 11 (October 22, 2021): 3718. http://dx.doi.org/10.3390/nu13113718.
Анотація:
The metaproteome profiling of cecal contents collected from neonatal piglets fed pasteurized human milk (HM) or a dairy-based infant formula (MF) from postnatal day (PND) 2 to 21 were assessed. At PND 21, a subset of piglets from each group (n = 11/group) were euthanized, and cecal contents were collected for further metaproteome analysis. Cecal microbiota composition showed predominantly more Firmicutes phyla and Lachnospiraceae family in the lumen of cecum of HM-fed piglets in comparison to the MF-fed group. Ruminococcus gnavus was the most abundant species from the Firmicutes phyla in the cecal contents of the HM-fed piglets at 21 days of age. A greater number of expressed proteins were identified in the cecal contents of the HM-fed piglets relative to the MF-fed piglets. Greater abundances of proteins potentially expressed by Bacteroides spp. such as glycoside enzymes were noted in the cecal lumen of HM-fed piglets relative to the MF. Additionally, lyases associated with Lachnospiraceae family were abundant in the cecum of the HM group relative to the MF group. Overall, our findings indicate that neonatal diet impacts the gut bacterial taxa and microbial proteins prior to weaning. The metaproteomics data were deposited into PRIDE, PXD025432 and 10.6019/PXD025432.
45
Mahé, Sylvain, Philippe Marteau, Jean-François Huneau, François Thuillier, and Daniel Tomé. "Intestinal nitrogen and electrolyte movements following fermented milk ingestion in man." British Journal of Nutrition 71, no. 2 (February 1994): 169–80. http://dx.doi.org/10.1079/bjn19940124.
Анотація:
The present study focuses on the digestion and absorption of milk and fermented milk (FM) reflected by gastro-ileal N and electrolyte movements in six healthy volunteers. The N and electrolyte content of the intestinal effluents were analysed both at the beginning of the jejunum and in the distal ileum. The gastric half-emptying time of the liquid phase was significantly (P< 0·05) shorter for milk (35 (SE 2) min) than for FM (60 (SE 2) min). The N balance showed that 58 and 50 % of ingested proteins, milk and FM respectively were absorbed between the stomach and the proximal jejunum and that 91 and 90% respectively were absorbed between the stomach and the terminal ileum in 240 min. Evaluation of mineral absorption indicated that 44 and 67% of Ca was absorbed in the duodenum after milk and FM ingestion respectively, and 41 and 11% of Ca disappeared between the jejunum and the ileum respectively. With regards to N and Ca intestinal availability, the present study confirms that FM products represent an interesting source of N as well as minerals for man. This confers on FM a beneficial effect compared with milk especially for lactase (EC3.2.1. 108)–deficient subjects and children with persistent diarrhoea.
46
Sørensen, Esben S., and Torben E. Petersen. "Purification and characterization of three proteins isolated from the proteose peptone fraction of bovine milk." Journal of Dairy Research 60, no. 2 (May 1993): 189–97. http://dx.doi.org/10.1017/s0022029900027503.
Анотація:
SummaryThree major proteins from the proteose peptone of bovine milk were purified by Sephadex G-75 gel chromatography, Q-Sepharose ion-exchange and additional Sephadex G-75 gel chromatography in the presence of urea. From their mobility in a gradient SDS-PAGE, the proteins were found to have molecular masses of 17, 28 and 60 kDa. The N-terminal amino acid sequence of the 17 kDa protein was found to be homologous with a camel whey protein. This protein has not previously been described in bovine milk. From the SDS-PAGE results, the 28 kDa protein was judged to be the major protein of proteose peptone, contributing ~ 25% of the total. The N-terminal amino acid sequence showed no homology to any known protein sequence, but the amino acid composition indicated that the 28 kDa protein is identical with the PP3 component from the proteose peptone fraction of bovine milk, or part of it. The 60 kDa protein was found to be bovine osteopontin, a very highly phosphorylated protein with an Arg-Gly-Asp sequence which mediates cell attachment.
47
Rio-Aige, Karla, Marina Girbal, Marta Selma-Royo, Anna Parra-Llorca, Sonia González, Cecilia Martínez-Costa, Margarida Castell, María Carmen Collado, Francisco J. Pérez-Cano, and María J. Rodríguez-Lagunas. "Galectins-1, -3 and -9 Are Present in Breast Milk and Have a Role in Early Life Development." Nutrients 14, no. 20 (October 17, 2022): 4338. http://dx.doi.org/10.3390/nu14204338.
Анотація:
Galectins (Gal) are a family of conserved soluble proteins with high affinity for β-galactoside structures. They have been recognized as important proteins for successful pregnancy. However, little is known about their presence in breast milk and their role in early infancy. Gal-1, -3 and -9 concentrations were evaluated by Multiplex immunoassays in mother–infant pairs from the MAMI cohort in maternal plasma (MP) (n = 15) and umbilical cord plasma (UCP) (n = 15) at birth and in breast milk samples (n = 23) at days 7 and 15 postpartum. Data regarding mother and infant characteristics were collected. Gal-9 was present in a lower concentration range than Gal-1 and Gal-3 in plasma, specifically in UCP. A major finding in the current study is that Gal-1, -3 and -9 were detected for the first time in all the transitional breast milk samples and no differences were found when comparing the two breastfeeding time points. Finally, Gal levels were associated with some maternal and infant characteristics, such as gestational age, pregnancy weight gain, maternal diet, the gender, infant growth and infant infections. In conclusion, Gal levels seem to be involved in certain developmental aspects of early life.
48
Feltrin, C., L. C. Garas, C. A. Cooper, K. Hamilton, R. V. L. Filho, L. R. Bertolini, M. Bertolini, H. E. Raybould, J. D. Murray, and E. A. Maga. "219 EFFECTS OF ADMINISTRATION OF MILK FROM TRANSGENIC COWS CONTAINING RECOMBINANT HUMAN LACTOFERRIN IN A PIG MODEL OF MALNUTRITION." Reproduction, Fertility and Development 26, no. 1 (2014): 223. http://dx.doi.org/10.1071/rdv26n1ab219.
Анотація:
Infant mortality is still a major problem, with the interaction between malnutrition and diarrhoea among the leading causes of death. One option to fight both diarrhoea and malnutrition is breastfeeding. Benefits of breast milk are attributed to the actions of antimicrobial proteins in human milk, such as lactoferrin (LF), which increase intestinal and systemic immune functions. One way to convey the benefits of LF to children is the use of transgenic animals that express human proteins in the mammary gland. In this sense, the availability of animal milk with properties of human milk can be a potential source to increase and prolong the protective benefits of human milk in reducing disease and stimulating growth. Transgenic cows expressing rhLF were produced by pronuclear microinjection with the goal of using the milk to improve human health. To test this hypothesis, we have created a model of malnutrition in pigs by reducing the intake (50%) of calories and protein. The animals (n = 26) were randomly divided as follows: after weaning at 3 weeks of age, 18 animals were fed the protein and calorie-restricted diet (mal) for 3 weeks and 8 animals served as a control group and were fed standard feed (full-fed). After 3 weeks, 4 animals in each group were necropsied and the remaining animals (n = 18) were placed into the following experimental groups: 4 animals remained in the control group (full-fed-no milk), and the 14 malnourished animals were divided as follows: 4 animals were maintained on food restriction but received no milk (mal-no milk) and 10 animals were maintained on food restriction with 5 receiving 500 mL of control milk/day (con milk) and 5 receiving 500 mL of rhLF milk/day (rhLF milk) for a total of 15 days. Intestinal permeability and morphology, mRNA expression of tight junction proteins (ZO1, claudin, occludin), and cytokines (TGF-β, TLR-4, IL-10, TNF-α, IL-6 IL-8, CCL-11) in the intestine, and hematological parameters were assessed. Data were analysed by ANOVA with P-values <0.05 considered statistically significant. The restricted diet was capable of inducing a state of malnutrition after 3 weeks as demonstrated by multiple changes in blood chemistry, a significant decrease in gut surface area, and an increase in electrical conductance indicative of compromised intestinal barrier function. Supplementation of the diet with either control milk or rhLF milk promoted the recovery of the intestine as indicated by significantly improved intestinal morphology and permeability. Levels of TNF-α were increased in the mal-no milk group; however, rhLF-fed animals were capable of regulating the expression of TNF-α, which did not significantly differ from full-fed controls. Tight junction proteins were also significantly up-regulated in the rhLF group. Overall, a model of malnutrition was established and the administration of both control and rhLF milk was beneficial in the recovery of the gastrointestinal tract. Our intention is that such milk from transgenic animals can benefit malnourished children around the world.
49
Hernández, M. J. Medina, E. Bonet Domingo, R. M. Villanueva Camañas, and M. C. García Alvarez-Coque. "Use of the o-Phthalaldehyde and N-Acetyl-L-Cysteine the Evaluation of Milk Proteins." Journal of Dairy Science 74, no. 6 (June 1991): 1779–85. http://dx.doi.org/10.3168/jds.s0022-0302(91)78342-2.
50
Yang, Yongxin, Nan Zheng, Xiaowei Zhao, Yangdong Zhang, Rongwei Han, Shengguo Zhao, Jinhui Yang, et al. "N-glycosylation proteomic characterization and cross-species comparison of milk whey proteins from dairy animals." PROTEOMICS 17, no. 9 (May 2017): 1600434. http://dx.doi.org/10.1002/pmic.201600434.