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Artigos de revistas sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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LAALA, Samia, Sophie CESBRON, Mohamed KERKOUD, Franco VALENTINI, Zouaoui BOUZNAD, Marie-Agnès JACQUES e Charles MANCEAU. "Characterization of Xanthomonas campestris pv. campestris in Algeria". Phytopathologia Mediterranea 60, n.º 1 (13 de maio de 2021): 51–62. http://dx.doi.org/10.36253/phyto-11726.
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Xanthomonas campestris pv. campestris (Xcc) causes the black rot of cruciferous plants. This seed-borne bacterium is considered as the most destructive disease to cruciferous crops. Although sources of contamination are various, seeds are the main source of transmission. Typical symptoms of black rot were first observed in 2011 on cabbage and cauliflower fields in the main production areas of Algeria. Leaf samples displaying typical symptoms were collected during 2011 to 2014, and 170 strains were isolated from 45 commercial fields. Xcc isolates were very homogeneous in morphological, physiological and biochemical characteristics similar to reference strains, and gave positive pathogenicity and molecular test results (multiplex PCR with specific primers). This is the first record of Xcc in Algeria. Genetic diversity within the isolates was assessed in comparison with strains isolated elsewhere. A multilocus sequence analysis based on two housekeeping genes (gyrB and rpoD) was carried out on 77 strains representative isolates. The isolates grouped into 20 haplotypes defined with 68 polymorphic sites. The phylogenetic tree obtained showed that Xcc is in two groups, and all Algerian strains clustered in group 1 in three subgroups. No relationships were detected between haplotypes and the origins of the seed lots, the varieties of host cabbage, the years of isolation and agroclimatic regions.
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Thieme, Frank, Ralf Koebnik, Thomas Bekel, Carolin Berger, Jens Boch, Daniela Büttner, Camila Caldana et al. "Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence". Journal of Bacteriology 187, n.º 21 (1 de novembro de 2005): 7254–66. http://dx.doi.org/10.1128/jb.187.21.7254-7266.2005.
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ABSTRACT The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oryzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophila and Coxiella burnetii. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.
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Ferreira, Polliana Silva Franco, e Nilvanira Donizete Tebaldi. "Métodos de inoculação de Xanthomonas campestris pv. passiflorae em maracujazeiro e biofertilizantes na inibição do crescimento bacteriano in vitro". Summa Phytopathologica 45, n.º 2 (abril de 2019): 207–9. http://dx.doi.org/10.1590/0100-5405/185793.
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RESUMO A mancha bacteriana do maracujazeiro (Passiflora spp.) causada por Xanthomonas campestris pv. passiflorae é uma das principais doenças que afeta a cultura. Para a obtenção de variedades resistentes à bactéria, vários métodos de inoculação devem ser testados. O objetivo do trabalho foi avaliar diferentes métodos de inoculação de Xanthomonas campestris pv. passiflorae em plantas de maracujá, para a obtenção de genótipos resistentes à bactéria e avaliar o efeito inibitório de biofertilizantes no crescimento bacteriano in vitro. Sete genótipos de maracujá foram inoculados com uma suspensão bacteriana (1x108 UFC.mL-1), via aspersão, tesoura e pinça. Os bioferlizantes Agro-Mos, Cop-R-Quik, FitoForce Plus, e Soil-Set foram avaliados quanto a inibição do crescimento bacteriano in vitro. O método de inoculação por aspersão foi o mais prático e rápido, em relação aos demais, na obtenção dos sintomas da doença e todos os genótipos avaliados foram suscetíveis à Xanthomonas campestris pv. passiflorae. Os biofertilizantes FitoForce Plus e Soil-Set inibiram o crescimento de Xanthomonas campestris pv. passiflorae in vitro e deverão ser avaliados para o controle da bactéria em condições de campo.
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Lee, Yung-An, Pei-Yu Yang e Shau-Chang Huang. "Characterization, Phylogeny, and Genome Analyses of Nonpathogenic Xanthomonas campestris Strains Isolated from Brassica Seeds". Phytopathology® 110, n.º 5 (maio de 2020): 981–88. http://dx.doi.org/10.1094/phyto-08-19-0319-r.
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Xanthomonads were detected by using the Xan-D(CCF) medium from the brassica seeds, and their pathogenicity was determined by plant inoculation tests. It was found that some seed lots were infested with Xanthomonas campestris pv. campestris, some with X. campestris pv. raphani, and some with nonpathogenic xanthomonads. The nonpathogenic xanthomonad strains were identified as X. campestris, and the multilocus sequence analysis showed that the nonpathogenic X. campestris strains were grouped together with pathogenic X. campestris, but not with nonpathogenic strains of X. arboricola. In addition, all isolated X. campestris pv. campestris and X. campestris pv. raphani strains were positive in the hrpF-PCR, but the nonpathogenic strains were negative. It was further found that nonpathogenic X. campestris strain nE1 does not contain the entire pathogenicity island (hrp gene cluster; type III secretion system) and all type III effector protein genes based on the whole genome sequence analyses. The nonpathogenic X. campestris strain nE1 could acquire the entire pathogenicity island from the endemic X. campestris pv. campestris and X. campestris pv. raphani strains by conjugation, but type III effector genes were not cotransferred. The studies showed that the nonpathogenic X. campestris strains indeed exist on the brassica seeds, but it could be differentiated by the PCR assays on the hrp and type III effector genes. Nevertheless, the nonpathogenic X. campestris strains cannot be ignored because they may be potential gene resources to increase genetic diversity in the endemic pathogenic X. campestris pv. campestris and X. campestris pv. raphani strains.
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Norman, D. J., J. M. F. Yuen e N. C. Hodge. "New Disease on Ornamental Asparagus Caused by Xanthomonas campestris in Florida". Plant Disease 81, n.º 8 (agosto de 1997): 847–50. http://dx.doi.org/10.1094/pdis.1997.81.8.847.
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From dark, water-soaked lesions on stems of asparagus tree fern (Asparagus virgatus) in commercial nurseries in Florida, 33 xanthomonad strains were isolated. Strains formed large, round, butyrus, bright yellow colonies on yeast dextrose calcium carbonate medium, and were gram negative, oxidase negative, catalase positive, motile, strictly aerobic, and did not hydrolyze starch. Strains were further characterized by carbon substrate utilization patterns (Biolog), and by fatty acid methyl esters (FAME) analyses. The metabolic fingerprints of most strains were similar to Xanthomonas campestris pv. vitians, and X. campestris pv. dieffenbachiae from Xanthosoma or Syngonium. Representative strains from A. virgatus were not pathogenic on Dieffenbachia. X. campestris pv. dieffenbachiae strains that did not hydrolyze starch produced scattered lesions on A. virgatus stems. However, starch-hydrolyzing strains of X. campestris pv. dieffenbachiae did not produce symptoms when inoculated onto A. virgatus. FAME analysis indicated the strains were X. campestris pv. vitians or X. campestris pv. translucens; however, low similarity indices ( x = 0.461) indicated that the asparagus strains were not represented in the MIDI library database. FAME analysis profiles were also compared to the University of Florida database, which contains 1,048 X. campestris strains of which 200 are X. campestris pv. dieffenbachiae. Similarity indices were again low with 15 strains matched to X. campestris pv. secalis (x = 0.412), seven strains to X. fragariae (x = 0.224), six strains to X. campestris pv. translucens ( x = 0.437), and five strains matched < 0.20 to other pathovars. Five representative strains were tested on six Asparagus species or cultivars: A. virgatus, A. setaceus, A. macowanii, A. densiflorus ‘Sprengeri’ , A. densiflorus ‘Myers’, and A. officinalis. All five strains were pathogenic on A. virgatus but were less virulent on A. setaceus and A. densiflorus ‘Sprengeri’.
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Manceau, Charles, Louis Gardan e Martine Devaux. "Dynamics of RP4 plasmid transfer between Xanthomonas campestris pv. corylina and Erwinia herbicola in hazelnut tissues, in planta". Canadian Journal of Microbiology 32, n.º 11 (1 de novembro de 1986): 835–41. http://dx.doi.org/10.1139/m86-154.
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We have shown that the transfer of plasmid RP4 took place between two strains of Xanthomonas campestris pv. corylina and from Xanthomonas campestris pv. corylina to Erwinia herbicola and vice versa when the strains were inoculated in hazelnut tissues in an experimental orchard. Transfer occurred throughout all seasons and was independent of the physiological conditions of the host tissues. The main factor governing transfer in planta appeared to be the frequency of contacts among donors and recipients. The frequency of transfer was correlated with the level of bacterial populations. RP4 was stable in the inoculated strains of Xanthomonas campestris pv. corylina as well as in Erwinia herbicola in hazelnut tissue. RP4 was detected in two isolates of resident epiphytic microflora, Pseudomonas fluorescens and Erwinia herbicola.
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Monteiro, Leila, Rosa de Lima Ramos Mariano e Ana Maria Souto-Maior. "Antagonism of Bacillus spp. against Xanthomonas campestris pv. campestris". Brazilian Archives of Biology and Technology 48, n.º 1 (janeiro de 2005): 23–29. http://dx.doi.org/10.1590/s1516-89132005000100004.
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The antagonism of eight Bacillus isolates was investigated against nine strains of Xanthomonas campestris pv. campestris (causal agent of crucifers black rot) to assess the role of lipopeptides in this process. Antimicrobial and hemolytic (surfactant) activity tests were performed in vitro using agar diffusion methods. Antibiosis and hemolysis were positive for four Bacillus isolates against all X. campestris pv. campestris strains. The correlation observed between antimicrobial and hemolytic activities indicated that lipopeptides were involved in the antibiosis mechanism of the studied antagonists. Fermentation studies were carried out with the isolates that showed highest antimicrobial and hemolytic activities, to follow up growth and production of bioactive and surfactant compounds. Production of bioactive and surfactant compounds was observed during the late growth phase of the Bacillus isolates.
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Vicente, J. G., J. Conway, G. J. King e J. D. Taylor. "RESISTANCE TO XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS IN BRASSICA SPP." Acta Horticulturae, n.º 539 (outubro de 2000): 61–67. http://dx.doi.org/10.17660/actahortic.2000.539.6.
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Zhang, Chunyan, Mingfa Lv, Wenfang Yin, Tingyan Dong, Changqing Chang, Yansong Miao, Yantao Jia e Yinyue Deng. "Xanthomonas campestris Promotes Diffusible Signal Factor Biosynthesis and Pathogenicity by Utilizing Glucose and Sucrose from Host Plants". Molecular Plant-Microbe Interactions® 32, n.º 2 (fevereiro de 2019): 157–66. http://dx.doi.org/10.1094/mpmi-07-18-0187-r.
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The plant pathogen Xanthomonas campestris pv. campestris produces diffusible signal factor (DSF) quorum sensing (QS) signals to regulate its biological functions and virulence. Our previous study showed that X. campestris pv. campestris utilizes host plant metabolites to enhance the biosynthesis of DSF family signals. However, it is unclear how X. campestris pv. campestris benefits from the metabolic products of the host plant. In this study, we observed that the host plant metabolites not only boosted the production of the DSF family signals but also modulated the expression levels of DSF-regulated genes in X. campestris pv. campestris. Infection with X. campestris pv. campestris induced changes in the expression of many sugar transporter genes in Arabidopsis thaliana. Exogenous addition of sucrose or glucose, which are the major products of photosynthesis in plants, enhanced DSF signal production and X. campestris pv. campestris pathogenicity in the Arabidopsis model. In addition, several sucrose hydrolase–encoding genes in X. campestris pv. campestris and sucrose invertase–encoding genes in the host plant were notably upregulated during the infection process. These enzymes hydrolyzed sucrose to glucose and fructose, and in trans expression of one of these enzymes, CINV1 of A. thaliana or XC_0805 of X. campestris pv. campestris, enhanced DSF signal biosynthesis in X. campestris pv. campestris in the presence of sucrose. Taken together, our findings demonstrate that X. campestris pv. campestris applies multiple strategies to utilize host plant sugars to enhance QS and pathogenicity.
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Kong, Congcong, Vânia Horta de Passo, Zhiyuan Fang, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Joana G. Vicente e Honghao Lv. "Complete Genome Sequence of Strain WHRI 3811 Race 1 of Xanthomonas campestris pv. campestris, the Causal Agent of Black Rot of Cruciferous Vegetables". Molecular Plant-Microbe Interactions® 32, n.º 12 (dezembro de 2019): 1571–73. http://dx.doi.org/10.1094/mpmi-07-19-0177-a.
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Xanthomonas campestris pv. campestris is an important bacterial pathogen that causes black rot and brings about enormous production loss for cruciferous vegetables worldwide. Currently, genome sequences for only a few X. campestris pv. campestris isolates are available, most of which are draft sequences. Based on the next-generation sequencing and single-molecule sequencing in real time technologies, we present here the complete genome sequence of strain WHRI 3811 race 1 of X. campestris pv. campestris, which is a type strain that has been extensively used. The genome data will contribute to our understanding of X. campestris pv. campestris genomic features and pave the way for research on X. campestris pv. campestris–host interactions.
Teses / dissertações sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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Garin, Tiffany. "Impact des compétitions inter-microbiennes médiées par le Système de Sécrétion de Type VI (T6SS) sur la dynamique de transmission du microbiote des graines aux plantules". Electronic Thesis or Diss., Angers, 2024. https://dune.univ-angers.fr/documents/dune18434.
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Les graines constituent la source d’inoculum initiale pour le microbiote des plantes. Lors de la germination, le relargage d’exsudats à proximité des graines déclenche une intense compétition microbienne qui joue un rôle moteur dans l’assemblage du microbiote des plantes. Ce travail de thèse vise à explorer le rôle de la compétition d'interférence médiée par le système de sécrétion de type VI (T6SS). Le T6SS d’une souche de Stenotrophomonas rhizophila, une espèce bactérienne transmise efficacement aux plantules, a été utilisé comme modèle d’étude. Ce T6SS est impliqué dans l’inhibition de la croissance de nombreuses espèces bactériennes in vitro, dont la souche phytopathogène Xanthomonas campestris pv. campestris 8004 (Xcc8004). Cette inhibition de croissance bactérienne est corrélée à la proximité phylogénétique et métabolique entre S. rhizophilaet les espèces bactériennes testées. L’activité antimicrobienne du T6SS de S. rhizophila limite la transmission de Xcc8004 de la graine de radis à la plantule en conditions gnotobiotiques. Cette activité module également la dynamique d’assemblage des communautés bactériennes lors de la transition graine-plantule en sol non stérile. La composition du consortium microbien co-inoculé sur graine influence l’avantage compétitif conféré par le T6SS à S. rhizophila. La compétition d’interférence médiée par le T6SS intervient donc bien dans la dynamique d’assemblage du microbiote des plantes et l'inoculation de consortiums bactériens composés de bactéries porteuses de T6SS apparait comme une solution potentielle de réduction de la transmission des agents phytopathogènes aux plantulesSeeds represent the initial inoculum source for plant microbiota. During germination, the release of exudates near the seeds triggers intense microbial competitions, which play a pivotal role in the assembly of the plant microbiota. This thesis work aims to explore the role of interference competition mediated by the Type VI secretion system (T6SS). The T6SS of a strain of Stenotrophomonas rhizophila, a bacterium efficiently transmitted to seedlings, was used as a study model. This T6SS inhibits the growth of many bacterial species in vitro, including the phytopathogenic strain Xanthomonas campestris pv. campestris 8004 (Xcc8004). This inhibition of bacterial growth is correlated with the phylogenetic and metabolic proximity between S. rhizophila and the tested bacterial species. The antimicrobial activity of S. rhizophila T6SS limits the transmission of Xcc8004 from radish seed to seedling under gnotobiotic conditions. Its activity also influences the dynamics of bacterial community assembly during the seed-to-seedling transition in non sterile soil. The composition of the co-inoculated microbial consortium on the seed affects the competitive advantage conferred by the T6SS to S. rhizophila. Therefore, T6SS-mediated interference competition plays a significant role in the assembly dynamics of the plant microbiota, and the inoculation of bacterial consortia composed of T6SS-carrying bacteria appears as a potential solution for reducing the transmission of phytopathogenic agents to seedlings
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DE, CRECY-LAGARD VALERIE. "Transport et metabolisme du fructose chez xanthomonas campestris pv. Campestris". Paris 7, 1991. http://www.theses.fr/1991PA077025.
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Xanthomonas campestris pv. Campestris est une bacterie phytopathogene qui secrete un polysaccharide, le xanthane. Le but de ce travail etait l'etude de la regulation du transport des sourcs de carbone chez x. Campestris. Les regulations trouvees chez e. Coli etaient-elles conservees ou existait-il d'autres mecanismes de regulation dans cette bacterie? chez e. Coli, deux systemes de regulation du transport des sources de carbone ont ete etudies en detail: le controle transcriptionnel par l'amp-cyclique associe a sa proteine receptrice cap; la regulation par le systeme des phosphotransferases dependantes du phosphoenolpyruvate (pts). Une proteine de x. Campestris similaire a la proteine cap d'e. Coli a ete isolee. Cette proteine cap, a la difference de celle d'e. Coli, ne regule pas chez x. Campestris l'expression des operons cataboliques. Nous avons montre que cette proteine est impliquee dans la regulation des facteurs de phytopathogenicite. Le systeme pts de x. Campestris a ete caracterise. Ce pts est specifique du fructose. Des mutants pts ont ete isoles, les genes du pts fructose clones et sequences. L'operon fructose de x. Campestris contient au moins trois genes: le premier, frub, code pour une proteine multifonctionnelle portant les trois activites eiii, pseudo-hpr et ei; le deuxieme, fruk, code pour la 1-phosphofructokinase et le troisieme, frua, code pour l'eii#f#r#u. Bien qu'il soit specifique du glucose, le pts de x. Campestris joue comme le pts plus general d'e. Coli un role essentiel dans l'utilisation des sources de carbone
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Drouin, François. "Caractérisation de l'intégron de Xanthomonas campestris pv badrii". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0019/MQ56399.pdf.
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Monteiro, Leila. "Produção de Substaâncias biotivas de Bacillus spp. contra Xanthomonas campestris pv. campestris". Universidade Federal de Pernambuco, 2002. https://repositorio.ufpe.br/handle/123456789/1304.
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Previous issue date: 2002O gênero Bacillus é um dos mais utilizados no biocontrole de doenças de plantas. São microrganismos encontrados facilmente em solos e plantas, que formam esporos tolerantes ao calor e à dessecação, o que facilita sua comercialização e estoque. O principal mecanismo de ação desses organismos no controle de fitopatógenos é a produção de substâncias antimicrobianas, entre as quais os lipopeptídeos, que apresentam também atividade hemolítica. Dentre os fitopatógenos que sofrem inibição por Bacillus spp., está a bactéria Xanthomonas campestris pv. campestris, causadora da podridão negra das crucíferas, doença de abrangência mundial, responsável por grandes perdas nas plantações de couve, repolho, nabo, rabanete, entre outras. Pesquisas anteriores mostraram que Bacillus spp. epifíticos, isolados de rabanete e couve, apresentam alta eficiência no controle da podridão negra em couve e repolho no campo. O presente estudo teve como objetivos a investigação de mecanismo de antibiose de oito isolados de Bacillus: B. subtilis R14, B. megaterium pv. cerealis RAB7, B. megaterium pv. cerealis C211, B. megaterium C116, Bacillus sp. RAB9, B. cereus C240, Bacillus sp. C11 e B. cereus C210, contra nove linhagens de X. campestris pv. campestris e a participação de lipopeptídeos neste mecanismo. Além disso, para os Bacillus que apresentaram resultados positivos de antibiose, foram realizados estudos de fermentação para acompanhar o crescimento e a produção de substâncias bioativas e tensoativas. Para o estudo de antibiose, foram realizados testes de atividade dos oito isolados contra as linhagens fitopatógenas de X. campestris pv. campestris, pelo método de difusão em ágar. Para verificar a produção de lipopeptídeos pelos Bacillus, foram realizados testes de hemólise em meio ágar sangue a 30o C e a 37o C. As fermentações foram realizadas em frascos de Fernbach, contendo 500 mL de meio de cultura a base de glicose e (NH4)2SO4, em mesa agitadora, a 150 rpm e 30°C. Os testes de atividade antimicrobiana se apresentaram positivos para todas as linhagens de X. campestris pv. campestris frente a quatro dos isolados de Bacillus testados: B. subtilis R14, B. megaterium pv. cerealis RAB7, B. megaterium C116 e B. cereus C210, os quais foram também os que apresentaram halos de hemólise, principalmente a 37o C. Os isolados, B. subtilis R14 e B. megaterium pv. cerealis RAB7 se mostraram os mais eficientes no antagonismo contra as linhagens de X. campestris pv. campestris. A correlação observada entre a atividade antimicrobiana e a atividade hemolítica indica que lipopeptídeos estão envolvidos no mecanismo de antibiose dos isolados investigados. Nos estudos de fermentação, observou-se a produção de substâncias bioativas e surfactantes durante a fase de crescimento de B. subtilis R14, B. megaterium pv. cerealis RAB7 e B. megaterium C116. Assim como nos testes em meio sólido, maiores atividades antimicrobianas foram observadas nos cultivos de B. subtilis R14 e B. megaterium pv. cerealis RAB7
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Silva, Rafael Salomão da. "Bactérias de solos supressivos com atividade antimicrobiana sobre Xanthomonas campestris pv. campestris". Universidade Federal de Sergipe, 2016. https://ri.ufs.br/handle/riufs/3021.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESXanthomonas campestris pv. campestris is a phytopathogenic bacterium, the causative agent of black rot in crucifers. For the control of plant pathogens diseases, there is the use of bacteria with activity antagonistic to the pathogen. Recent studies show that Bacillus species have on X. campestris a strong biological control. One of the mechanisms of this control is the production of secondary metabolites by these species. The objective of this work was to select bacteria X. campestris and antagonists to evaluate the antimicrobial activity of extracellular filtered bacteria (FEB) antagonist activity. To this, 257 bacteria isolated from a suppressive soil. They were evaluated in vitro antagonist activity by the technique of double layer. Ninety-two isolates (44.6%) were able to inhibit growth of the target pathogen (X.campestris). Of the 92 isolates selected on double layer of the test, 51 (55.43%) showed inhibition of growth of X. campestris on the inhibition assays with FEB in liquid medium. Thirteen of 50% or more inhibited the growth of the target pathogen, and the FEB-8, FEB-31-FEB 68, FEB 74-FEB-87 and were able to inhibit 100% growth of X. campestris. The FEB isolated TC-DT08, belonging to the genus Paenibacillus, it was used for in vivo tests in plant farming kale. The artificial inoculation kale with X. campestris pretreated with FEB-08 showed that the bacterium loses the ability to colonize and cause the cabbage black rot, indicating the potential use of this isolate to protect kale butter infection by X. campestris.
Xanthomonas Campestris. pv campestris é uma bactéria fitopatogênica, agente causal da podridão negra em crucíferas. Dentre os mecanismos para o controle de doenças de fitopatógenos, destaca-se o uso de bactérias com atividade antagonista ao patógeno. Estudos recentes mostram que espécies de Bacillus exercem sobre X. Campestris um forte controle biológico. Um dos mecanismos deste controle é a produção de metabólitos secundários por essas espécies. O objetivo deste trabalho foi selecionar bactérias antagonistas a X. campestris e avaliar a atividade antimicrobiana dos filtrados extracelulares das bactérias (FEB) com atividade antagonista. Para isso, 257 bactérias isoladas de solos supressivos foram avaliadas quanto a atividade antagonista in vitro pela técnica da dupla camada. Noventa e dois isolados (44,6%) foram capazes de inibir o crescimento do fitopatógeno alvo (X.campestris). Dentre os 92 isolados selecionados no teste da dupla-camada, 51 (55,43%) apresentaram inibição do crescimento da X. campestris nos ensaios de inibição com os FEB em meio líquido. Treze destes inibiram 50% ou mais do crescimento do fitopatógeno-alvo, sendo que os FEB-08, FEB-31, FEB-68, FEB-74 e FEB-87 foram capazes de inibir 100% do crescimento de Xanthomonas campestris. O FEB do isolado TC-DT08, pertencente ao gênero Paenibacillus, foi utilizado para testes in vivo em plantas de couve-manteiga, em condições de casa de vegetação. A inoculação artificial de couve-manteiga com X. campestris pré-tratada com o FEB-8 demonstrou que a bactéria perde a habilidade de colonizar a couve e causar a podridão negra, o que indica o potencial do uso deste isolado para proteger a couve-manteiga da infecção por X. campestris.
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Sanders, Gina Mercia. "Detection of Xanthomonas Campestris PV. magniferaeindicae in mango plants". Diss., University of Pretoria, 1993. http://hdl.handle.net/2263/39792.
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The yearly losses incurred by bacterial blackspot disease are high. Often trees are
asymptomatic, with the pathogen either in the resident phase or latent stage of infection.
Detection of the pathogen in these asymptomatic trees is one of the most important
means of controlling the disease. Isolates which consistently differed in virulence were
isolated from symptomatic mango plants. These isolates could be categorised into four
groups based upon differences in virulence. Monoclonal antibodies (mAbs) were
successfully raised using separate and pooled isolates for immunisation. MAbsraised were
of the lgG class and reacted with a proteinaceous epitope. These monoclonal antibodies
could distinguish between different virulence groups of Xanthomonas campestris pv.
mangiferaeindicae by means of Western Blot analysis. These antibodies were used along
with a selective medium, BVGA for detection of epiphytic populations as well as latent
infections in mango. An enrichment step prior to the enzyme- linked immunosorbent assay
(ELISA) is important, since bacterial counts on trees with latent infections are too low to
result in a positive signal. These techniques in combination are thus useful for detection
and monitoring of the pathogen, which may play an important role in controlling the
spread of the disease.Dissertation (MSc Agric)--University of Pretoria, 1993.
gm2014
Microbiology and Plant Pathology
Unrestricted
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Pigatto, Gisele [UNESP]. "Irradiação UV em Xanthomonas campestris pv. campestris visando a produção da goma xantana". Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/94867.
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pigatto_g_me_sjrp.pdf: 376138 bytes, checksum: 0d3df3ba13a94adf6610c9416261a91c (MD5)Xanthomonas campestris é uma bactéria fitopatogênica que causa a podridão negra no sistema vascular das plantas da família das cruciferaceaes. Produz um exopolissacarídeo denominado goma xantana, que possui propriedades reológicas únicas sendo utilizada amplamente como agente de suspensão, espessante, emulsionante e estabilizante. É aplicados em indústrias petrolíferas, alimentícias, farmacêuticas, mineração, têxtil, termoquímicas, tintas, cosméticos e produtos agropecuários. O Brasil é um grande produtor mundial de cana de açúcar e álcool etílico. Produtos estes utilizados para a produção de xantana; o primeiro como substrato da fermentação e o segundo para a separação da goma. Apesar de todo esse potencial, o Brasil importa grande quantidade de goma xantana que poderá ser produzida com grande competitividade internacional. Portanto, este trabalho objetivou a utilização da técnica de irradiação ultravioleta, em uma linhagem específica de Xanthomonas campestris, para a obtenção de mutantes estáveis que possam melhorar o rendimento e/ou qualidade de goma obtida. A quantificação foi realizada através da determinação da biomassa, viscosidade, cálculos do rendimento da biomassa e goma. A irradiação UV por 600 segundos causou uma redução de 92,2% na população irradiada e as linhagens sobreviventes foram isoladas e analisadas nos testes de produção e viscosidade da goma xantana. As linhagens I6, I7, I9 e I10 apresentaram um aumento de 102% na produção de goma comparando com a linhagem não irradiada. Em relação à viscosidade do caldo, as linhagens irradiadas obtiveram um aumento de 48% comparadas com as não irradiadas de 20 e 30 rpm. A viscosidade da solução de goma xantana 1%, também foram superiores quando comparadas com a não irradiada. O aumento de...
Xanthomonas campestris is fitopatogenic bacterium that causes the black rotten in the vascular system of the plants of the family of the cruciferaceaes. It produces an exopolysaccharides that forms the xanthan gum, which is used in ample variety as agent of suspension, thicker, emulsifier and stabilizing, and singular rheological properties. It is applied in petroliferous, nourishing, pharmaceutical industries, of mining, textile, thermo chemistries, inks, cosmetics and farming products. Brazil is the worldwide producing greater of sugar cane of sugar and ethyl alcohol. Products theses used for the xanthan production; the first one as substratum of the fermentation, and the second as for the separation of the gum. Despite all this potential, the Brazil imports lot of xanthan gum that could be produced with great international competitiveness. This aimming work the used of the ultraviolet technique of irradiation in a specific strain of Xanthomonas campestris to obtain the mutants that can improve the income and/or quality of produced gum, through the determination of the biomass, viscosity, calculations of the income of the biomass and gum. The UV irradiation during 60 seconds caused a reduce of the 92.2% in the irradiated strains and the survived strains were isolated and analysed in the tests of production and viscosity of xanthan gum. The strains I6, I7, I9 e I10 showed increased in the xanthan production of 102% comparing with the non-irradiated strain. In relation the viscosity of the broth the irradiated strains the increase of 48% in shear rate of 20 and 30 rpm compared with the no irrdiated. The viscosity of the xanthan solution 1% irradiated were higher also whwn compared with no irradiated in both shear rate (20 and 30 rpm. The increase of viscosity was of 17% to rotational speed of 20 rpm and 16% to 30 rpm. The ...(Complete abstract click electronic access below)
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Pigatto, Gisele. "Irradiação UV em Xanthomonas campestris pv. campestris visando a produção da goma xantana /". São José do Rio Preto : [s.n.], 2008. http://hdl.handle.net/11449/94867.
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Orientador: Pedro de Oliva NetoBanca: Valéria Marta Gomes de Lima
Banca: Ivanise Guilherme Branco
Resumo: Xanthomonas campestris é uma bactéria fitopatogênica que causa a podridão negra no sistema vascular das plantas da família das cruciferaceaes. Produz um exopolissacarídeo denominado goma xantana, que possui propriedades reológicas únicas sendo utilizada amplamente como agente de suspensão, espessante, emulsionante e estabilizante. É aplicados em indústrias petrolíferas, alimentícias, farmacêuticas, mineração, têxtil, termoquímicas, tintas, cosméticos e produtos agropecuários. O Brasil é um grande produtor mundial de cana de açúcar e álcool etílico. Produtos estes utilizados para a produção de xantana; o primeiro como substrato da fermentação e o segundo para a separação da goma. Apesar de todo esse potencial, o Brasil importa grande quantidade de goma xantana que poderá ser produzida com grande competitividade internacional. Portanto, este trabalho objetivou a utilização da técnica de irradiação ultravioleta, em uma linhagem específica de Xanthomonas campestris, para a obtenção de mutantes estáveis que possam melhorar o rendimento e/ou qualidade de goma obtida. A quantificação foi realizada através da determinação da biomassa, viscosidade, cálculos do rendimento da biomassa e goma. A irradiação UV por 600 segundos causou uma redução de 92,2% na população irradiada e as linhagens sobreviventes foram isoladas e analisadas nos testes de produção e viscosidade da goma xantana. As linhagens I6, I7, I9 e I10 apresentaram um aumento de 102% na produção de goma comparando com a linhagem não irradiada. Em relação à viscosidade do caldo, as linhagens irradiadas obtiveram um aumento de 48% comparadas com as não irradiadas de 20 e 30 rpm. A viscosidade da solução de goma xantana 1%, também foram superiores quando comparadas com a não irradiada. O aumento de...(Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Xanthomonas campestris is fitopatogenic bacterium that causes the black rotten in the vascular system of the plants of the family of the cruciferaceaes. It produces an exopolysaccharides that forms the xanthan gum, which is used in ample variety as agent of suspension, thicker, emulsifier and stabilizing, and singular rheological properties. It is applied in petroliferous, nourishing, pharmaceutical industries, of mining, textile, thermo chemistries, inks, cosmetics and farming products. Brazil is the worldwide producing greater of sugar cane of sugar and ethyl alcohol. Products theses used for the xanthan production; the first one as substratum of the fermentation, and the second as for the separation of the gum. Despite all this potential, the Brazil imports lot of xanthan gum that could be produced with great international competitiveness. This aimming work the used of the ultraviolet technique of irradiation in a specific strain of Xanthomonas campestris to obtain the mutants that can improve the income and/or quality of produced gum, through the determination of the biomass, viscosity, calculations of the income of the biomass and gum. The UV irradiation during 60 seconds caused a reduce of the 92.2% in the irradiated strains and the survived strains were isolated and analysed in the tests of production and viscosity of xanthan gum. The strains I6, I7, I9 e I10 showed increased in the xanthan production of 102% comparing with the non-irradiated strain. In relation the viscosity of the broth the irradiated strains the increase of 48% in shear rate of 20 and 30 rpm compared with the no irrdiated. The viscosity of the xanthan solution 1% irradiated were higher also whwn compared with no irradiated in both shear rate (20 and 30 rpm. The increase of viscosity was of 17% to rotational speed of 20 rpm and 16% to 30 rpm. The ...(Complete abstract click electronic access below)
Mestre
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Silva, Fernanda Pereira da. "Caracterização biológica e molecular de um bacteriófago específico para Xanthomonas campestris pv. campestris". Universidade Federal de Viçosa, 2015. http://www.locus.ufv.br/handle/123456789/6502.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Os vírus que infectam bactérias são denominados bacteriófagos, sendo também referidos como “fagos”. Os bacteriófagos que infectam bactérias fitopatogênicas, tem despertado crescente interesse devido ao seu potencial para o biocontrole. A bactéria Gram negativa Xanthomonas campestris pv. campestris, é o agente causal da podridão negra das brássicas (Brassicaceae), sendo responsável por perdas econômicas, resultantes do difícil controle. Assim, este trabalho teve como objetivo realizar o isolamento e a caracterização biológica e molecular de bacteriófagos infectando Xanthomonas campestris pv. campestris. Para isso, plantas da família Brassicaceae apresentando sintomas da podridão negra e solo rizosférico, foram coletados em campos de cultura em Coimbra-MG e triados para a presença de bacteriófagos adotando a técnica de formação de placas de lise por meio da sobrecamada de ágar. Das nove amostras analisadas, uma amostra apresentou placas de lise persistente nos quatro ciclos de propagação. O fago foi denominado Xacp1 e apresentou cabeça icosaédrica de aproximadamente 30 ± 5 nm de diâmetro e uma cauda curta com 6 ± 0,2 nm de comprimento e 7 ± 0,2 nm de diâmetro. O bacteriófago possui ácido nucleico composto por uma única molécula de DNA fita dupla (dsDNA) com tamanho estimado em 65 Kpb. De acordo com a morfologia e tipo de genoma, o bacteriófago Xacp1 foi classificado na família Podoviridae (Ordem Caudovirales). A sequencia do genoma completo do fago está sendo analisada e será utilizada para estudar o relacionamento filogenético com outros bacteriófagos que infectam Xanthomonas campestris. O bacteriófago mostrou capacidade em infectar apenas isolados de Xanthomonas vcampestris pv. campestris no teste de susceptibilidade, apresentando placas de lise com coloração, tamanho e formato padrão. Isolados bacterianos relacionados e não relacionados a Xanthomonas campestris pv. campestres não foram suscetíveis à infecção. Em conjunto esses resultados demostram que Xacp1 apresentou características ideais de especificidade e virulência que motivam futuros estudos para sua utilização como uma ferramenta biotecnológica para a utilização no biocontrole da podridão negra em brássicas.
Viruses that infect bacteria are termed bacteriophages, and also referred to as “phage”, or “bacteriovírus”. The bacteriophages that infect bactéria plant pathogens, has attracted increasing interest due to its potential for biocontrol. The gram-negative bacterium Xanthomonas campestris pv. campestris is causal agent of black rot of crucifers (Brassicaceae), accounting for economic losses resulting from difficult to control. This study had objective perform the isolation and molecular characterization of biological and bacteriophage infecting Xanthomonas campestris pv. campestris. For this, plants of the family Brassicaceae showing symptoms of black rot and rhizosphere soil were collected in crop fields in Coimbra-MG and screened for the presence bacteriophage adopting the technique of forming plaques by agar overlay. The analyzed nine samples, one sample showed signs of Persistent lysis in four cycles of the spread. The phage was named φXacp1 and icosahedral head showed approximately 30 ± 5 nm in diameter and a tail short-± 5 nm in length and 0.7 ± 0.3 4 nm diameter. The bacteriophage has nucleic acid consists of a single double stranded DNA molecule (dsDNA) with size estimated at 65 kbp. According to the morphology and type of genome, bacteriophage φXacp1 scored in Podoviridae family (Order Caudovirales). The sequence the complete genome of the phage is being analyzed and will be used to study the phylogenetic relationships with other bacteriophages that infect Xanthomonas campestris. The bacteriophage showed ability to infect only Xanthomonas campestris pv. campestris in susceptibility testing, presenting plaques staining, size and pattern shape. Isolated bacterial related and unrelated to Xanthomonas campestris. Country were not susceptible to infection. viiTogether these results demonstrate that φXacp1 presented ideal characteristics of specificity and virulence that motivate future studies for its use as a biotechnological tool for use in biocontrol of black rot in crucifers.
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Wruck, Dulândula Silva Miguel. "Análises bioquímica, patogênica, sorológica e molecular de isolados de Xanthomonas campestris pv. campestris". Universidade Federal de Viçosa, 2001. http://www.locus.ufv.br/handle/123456789/11176.
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Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Trinta e três isolados de Xanthomonas campestris pv. campestris, obtidos de oito diferentes brássicas, provenientes de diferentes regiões do Brasil e do exterior, foram analisados com base em caracterizações bioquímica, patogênica, sorológica e genética, objetivando verificar a existência ou não de relacionamentos quanto à origem geográfica e ao hospedeiro. No estudo bioquímico, foram realizados 22 testes, além da avaliação da atividade de esterase, em que com base no tamanho do halo e utilizando o teste de Scott Knott no nível de 5% de probabilidade, obtiveram-se cinco grupos de similaridade. Avaliando a capacidade de produção de bacteriocina, verificou-se que três isolados apresentaram antibiose contra sete outros. Avaliou-se, também, a sensibilidade dos isolados a alguns antibióticos e, com base na análise de agrupamento, foi possível a observação de três grupos distintos: o primeiro constituído por quatro isolados resistentes a 11 ou mais antibióticos; o segundo grupo composto por um isolado resistente somente a três antibióticos; e o terceiro grupo composto pelos demais isolados. Na caracterização patogênica, foi realizada a inoculação cruzada, de forma que os 33 isolados foram inoculados artificialmente em sete diferentes brássicas. Desses, 14 não mostraram especificidade quanto aos hospedeiros, enquanto os 19 isolados restantes apresentaram relativo grau de especificidade, uma vez que não causaram doença em uma ou mais das brássicas inoculadas. Para a análise sorológica, foram obtidos sete antissoros, pelos quais se reconheceram, no teste de imunodifusão dupla, 32 dos 33 isolados estudados, além de mostrarem especificidade quanto ao reconhecimento dos isolados de X. campestris pv. campestris. Na caracterização molecular, realizou-se a extração do DNA total de todos os isolados, seguida de amplificação pela técnica do RAPD. Procedeu-se, também, à extração do DNA plasmidial. Para avaliação do comportamento dos 33 isolados de X. campestris pv. campestris de diferentes origens e hospedeiros, com relação às diferentes análises realizadas, foram efetuadas análises estatísticas univariadas, para a avaliação da atividade de esterase, e multivariadas, para as demais, bem como a análise conjunta de todos os resultados. Com base nos resultados das caracterizações bioquímica, patogênica e sorológica, não se observou correlação da variabilidade dos isolados estudados com a origem geográfica e o hospedeiro do qual foram obtidos. Isso pode ter sido devido à constante introdução de novos isolados de X. campestris pv. campestris nas áreas de plantio, por meio de sementes contaminadas.
Thirty-three isolates of Xanthomonas campestris pv. campestris, obtained from eight different Brassica species and originated from distinct geographic regions in Brazil and other countries, were analyzed in terms of their biochemical, pathogenic, serological and molecular properties, in order to determine whether there is a relationship between isolate variability and host or geographic origin. Twenty-two biochemical tests, besides the determination of sterase activity, were carried out for the biochemical characterization. Five similarity groups were identified using Scott- Knot s test at 5% probability. The analysis of bacteriocin production indicated that three isolates displayed antibiosis against seven other isolates. Sensitivity to several antibiotics was also evaluated. Using cluster analysis, three distinct groups were identified: the first group consisted of four isolates which were resistant to 11 or more antibiotics; the second group consisted of one isolate which was resistant to only one antibiotic; the third group consisted of all the other remaining isolates. For the pathogenic characterization, all 33 isolates were cross-inoculated onto seven different Brassica species. Fourteen isolates did not display host specificity. The remaining 19 isolates displayed a relative degree of host specificity, since they did not induce disease in one or more of the inoculated species. Seven polyclonal antisera were raised for the serological analysis. In immunodiffusion tests, 32 out of the 33 isolates tested were recognized by these antisera. The antisera specifically recognized isolates of X. campestris pv. campestris. For the molecular characterization, total DNA was extracted from each isolate and used as a template for RAPD reactions. Plasmid DNA was also extracted. Together, the data from the biochemical, pathogenic, serological and molecular analyses failed to indicate a relationship among the isolates, hosts and geographic origin. This could be due to the repeated introduction of new isolates of X. campestris pv. campestris into different geographic regions, via contaminated seeds.
Livros sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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Liddle, Shona. Strategies for studying pathogenicity genes of Xanthomonas campestris pv. campestris. Norwich: Universityof East Anglia, 1992.
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Koch, Margery F. Aspects of quantative resistance to Xanthomonas campestris pv. oryzae in rice. Wageningen: Landbouwuniversiteit, 1990.
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Todd, Gordon Allan. The application of molecular genetics to the analysis of the interaction between Xanthomonas campestris PV. oryzaeand rice. Birmingham: University of Birmingham, 1987.
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Saunders, Barnett Edward. The development of a molecular genetic approach to identify alterations in rice ('oryza sativa') gene expression in response to challenge by 'Xanthomonas campestris' PV.'oryzae'. Birmingham: University of Birmingham, 1989.
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Canteros, Blanca Isabel. Diversity of plasmids and plasmid-encoded phenotypic traits in Xanthomonas campestris pv. vesicatoria. 1990.
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Schulte, Ralf. Molekulargenetische Analyse der hrp Gene von Xanthomonas campestris pv. vesicatoaia. 1992.
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Horns, Torsten. Untersuchungen zur Regulation der hrp Gene von Xanthomonas campestris pv. veslcatoria. 1994.
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Chang, L. W. H. Pests not known to occur in the United States or of limited distribution. 90. Xanthomonas campestris pv. oryzicola. 1987.
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Abdallah, Roshan. Evaluation of DNA hybridization probes for detecting Xanthomonas campestris pv. vesicatoria and analysis of genomic diversity by RFLP techniques. 1993.
Encontre o texto completo da fonteCapítulos de livros sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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Randhawa, P. S., e E. L. Civerolo. "Indigenous Plasmids in Xanthomonas Campestris Pv. Pruni". In Plant Pathogenic Bacteria, 465–69. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_92.
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Cook, D. R., e D. J. Robeson. "The Induction of Active Resistance in Cabbage to Black Rot (Xanthomonas Campestris Pv. Campestris) by Inoculation with Xanthomonas Campestris Pv. Carotae". In Plant Pathogenic Bacteria, 722–32. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_153.
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He, Ya-Wen, Wei Qian e Shan-Ho Chou. "Cyclic di-GMP Signaling in the Phytopathogen Xanthomonas campestris pv. campestris". In Microbial Cyclic Di-Nucleotide Signaling, 427–42. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-33308-9_25.
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Dow, J. M., e M. J. Daniels. "Pathogenicity Determinants and Global Regulation of Pathogenicity of Xanthomonas campestris pv. campestris". In Bacterial Pathogenesis of Plants and Animals, 29–41. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-642-78624-2_2.
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McGuire, R. G., e J. B. Jones. "Recovery of Xanthomonas Campestris Pv. Vesicatoria from Tomato Seed". In Plant Pathogenic Bacteria, 768. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_164.
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Fujimoto, D. K., e A. K. Vidaver. "Analysis of Strain Variation in Xanthomonas Campestris Pv. Phaseoli". In Plant Pathogenic Bacteria, 797. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_174.
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Stall, R. E. "Plasmid-Specified Host Specificity in Xanthomonas Campestris Pv. Vesicatoria". In Plant Pathogenic Bacteria, 1042–50. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_226.
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van Hulten, Marieke, Sayantani Chatterjee e Harrold A. van den Burg. "Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes". In Methods in Molecular Biology, 159–85. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9458-8_16.
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Haas, J. M., W. F. Fett e D. J. Fleming. "Detection and Initial Characterization of Plasmids in Xanthomonas Campestris Pv. Glycines". In Plant Pathogenic Bacteria, 479. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_102.
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Verma, J. P., S. G. Borkar e R. P. Singh. "Interaction between Genotypes of Xanthomonas Campestris Pv. Malvacearum and Gossypium Hirsutum". In Plant Pathogenic Bacteria, 639. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_139.
Texto completo da fonteTrabalhos de conferências sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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Kuznetsov, M. A., A. A. Scherbakov, S. V. Ivashchenko, E. A. Gorelnikova e N. S. Chervyakova. "Identification black rot pathogen Xanthomonas campestris pv. campestris with biochemical tests". In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.147.
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"Plasmid genome dynamics of sequenced strains of Xanthomonas campestris pv. campestris". In Bioinformatics of Genome Regulation and Structure/Systems Biology (BGRS/SB-2022) :. Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, 2022. http://dx.doi.org/10.18699/sbb-2022-096.
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Щербакова, Татьяна. "Влияние биопрепаратов на основе Trichoderma на снижение развития сосудистого бактериоза капусты". In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.87.
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Biological preparations Gliocladin-SC and Trichodermin-SC based on the genus Trichoderma fungi were used to protect white cabbage from vascular bacteriosis (pathogen Xanthomonas campestris pv. campestris). The preparations were used by watering plants with a 1,5% aqueous suspension. The biological effectiveness of the biological product Gliocladin-SC for 2 years of research was 79,6%, the biological product Trichodermin-SC – 67,1%.
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TRINDADE, R. A., A. P. MUNHOZ e C. A. V. BURKERT. "CARACTERIZAÇÃO DA XANTANA PRODUZIDA POR Xanthomonas campestris pv. mangiferaeindicae IBSBF 1230 UTILIZANDO DIFERENTES FONTES DE CARBONO". In XX Congresso Brasileiro de Engenharia Química. São Paulo: Editora Edgard Blücher, 2015. http://dx.doi.org/10.5151/chemeng-cobeq2014-0882-22847-152681.
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Orynbayev, A. T., K. A. Miroshnikov, A. N. Ignatov e F. S. Dzhalilov. "Evaluation of effectiveness of bacteriophage agent for cabbage black rot control". In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-113.
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Twenty-one isolates of bacteriophages specific to eleven target strains of Xanthomonas campestris pv. campestris were isolated from soil samples collected under black rot-infected cabbage plants. After the analysis of phagotyping for seventy-three phytopathogen strains against newly isolated isolates and four collection strains of bacteriophages, it was proposed to construct a phage cocktail including 6 isolates In vitro screening of protective from ultraviolet radiation substances under the UV-B range showed that skim milk (0.75%), Riboflavin (0.5%) and ascorbic acid (0.1%) showed the highest effect for bacteriophages. Under the conditions of a film greenhouse, the best protective effect from solar UV radiation on the 8th day after spraying cabbage was shown by the option with the addition of skimmed milk (0.75%) and Riboflavin (0.5%). According to our data, these substances can provide a long-term photoprotective effect of the bacteriophage preparation..
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Kender, Walter J. "Citrus Canker: Impacts of Research on Eradication and Control". In ASME 1986 Citrus Engineering Conference. American Society of Mechanical Engineers, 1986. http://dx.doi.org/10.1115/cec1986-3204.
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Citrus Bacterial Canker Disease (CBCD), caused by Xanthomonas campestris pv. citri, occurs in many citrus areas of the world. It has been reported in 40 different countries, on 5 continents (Asia, South Africa, Australia, South America and North America). Prior to the 1984 outbreak in Florida, the 4 known strains of the bacterium were A, B, C and Mexican bacterioses. Canker-A or Strain-A, endemic in Asia, was reported in China, India and Java in the early 1800’s, found in Japan in 1899 and in the Philippines in 1914. It affects most citrus species and hybrids. Grapefruit is especially susceptible. Strain-A was introduced into the United States from Japan on trifoliate orange seedlings in 1910. An eradication program was started in 1915 in Florida and the disease was eradicated in 1927. In South America, the Asiatic form was not found until 1957 in Brazil and 1972 in Argentina. In 1979, the A Strain broke out in the commercial citrus area of Sao Paulo State, Brazil. Paper published with permission.
Relatórios de organizações sobre o assunto "Xanthomonas camXanthomonaspestris pv. campestris"
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van der Wolf, Jan, Pieter Kastelein, Leo Poleij, Patricia van der Zouwen, Marjon Krijger, Odette Mendes, Jan Bergervoet, Bernadette Kroon, Pauline Bernardo e Reindert Nijland. Mapping tracks of Xanthomonas campestris pv. campestris resulting in Brassica seed infections. Wageningen: Stichting Wageningen Research, Wageningen Plant Research, Business unit Biointeractions and Plant Health, 2020. http://dx.doi.org/10.18174/536441.
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Michel Jr., Frederick C., Harry A. J. Hoitink, Yitzhak Hadar e Dror Minz. Microbial Communities Active in Soil-Induced Systemic Plant Disease Resistance. United States Department of Agriculture, janeiro de 2005. http://dx.doi.org/10.32747/2005.7586476.bard.
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Induced Systemic Resistance (ISR) is a highly variable property that can be induced by compost amendment of potting media and soils. For example, previous studies showed that only 1 of 79 potting mixes prepared with different batches of mature composts produced from several different types of solid wastes were able to suppress the severity of bacterial leaf spot of radish caused by Xanthomonas campestris pv. armoraciae compared with disease on plants produced in a nonamended sphagnum peat mix. In this project, microbial consortia in the rhizosphere of plants grown in ISR-active compost-amended substrates were characterized. The plants used included primarily cucumber but also tomato and radish. Rhizosphere microbial consortia were characterized using multiple molecular tools including DGGE (Israel) and T -RFLP (Ohio) in both ISR-active field plots and potting media. Universal as well as population-specific bacterial and fungal PCR primers were utilized. T -RFLP analyses using universal bacterial primers showed few significant differences in overall bacterial community composition in ISR-active and inactive substrates (Ohio). In addition, the community members which were significantly different varied when different ISR-activecomposts were used (Ohio). To better characterize the shifts in microbial community structure during the development of ISR, population specific molecular tools were developed (Israel, Ohio).-PCR primers were designed to detect and quantify bacterial groups including Pyrenomycetes, Bacillus, Pan toea, Pseudomonas, Xanthomonas and Streptomyces as well as Trichoderma and Fusarium; two groups of fungi that harbor isolates which are ISR active (Isreal and Ohio). Bacterial consortia associated with cucumber plants grown in compost-amended potting mixtures were shown to be dominated by the phylogenetic taxon Bacteroidetes, including members of the genus Chryseobacterium, which in some cases have been shown to be involved in biocontrol (Israel). Nested-PCR-DGGE analyses coupled with long l6S rDNA sequencing, demonstrated that the Chryseobacteriumspp. detected on seed and the root in compost-amended treatments were derived from the compost itself. The most effective ISR inducing rhizobacterial strains were identified as Bacillus sp. based on partial sequencing of l6S rDNA. However, these strains were significantly less effective in reducing the severity of disease than Trichoderma hamatum382 (T382). A procedure was developed for inoculation of a compost-amended substrate with T -382 which consistently induced ISR in cucumber against Phytophthora blight caused by Phytophthora capsiciand in radish against bacterial spot (Ohio). Inoculation of compost-amended potting mixes with biocontrol agents such as T -382 and other microbes that induce systemic resistance in plants significantly increased the frequency of systemic disease control obtained with natural compost amendments.
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Sessa, Guido, e Gregory Martin. A functional genomics approach to dissect resistance of tomato to bacterial spot disease. United States Department of Agriculture, janeiro de 2004. http://dx.doi.org/10.32747/2004.7695876.bard.
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The research problem. Bacterial spot disease in tomato is of great economic importance worldwide and it is particularly severe in warm and moist areas affecting yield and quality of tomato fruits. Causal agent of spot disease is the Gram-negative bacterium Xanthomonas campestris pv. vesicatoria (Xcv), which can be a contaminant on tomato seeds, or survive in plant debris and in association with certain weeds. Despite the economic significance of spot disease, plant protection against Xcvby cultural practices and chemical control have so far proven unsuccessful. In addition, breeding for resistance to bacterial spot in tomato has been undermined by the genetic complexity of the available sources of resistance and by the multiple races of the pathogen. Genetic resistance to specific Xcvraces have been identified in tomato lines that develop a hypersensitive response and additional defense responses upon bacterial challenge. Central goals of this research were: 1. To identify plant genes involved in signaling and defense responses that result in the onset of resistance. 2. To characterize molecular properties and mode of action of bacterial proteins, which function as avirulence or virulence factors during the interaction between Xcvand resistant or susceptible tomato plants, respectively. Our main achievements during this research program are in three major areas: 1. Identification of differentially expressed genes during the resistance response of tomato to Xcvrace T3. A combination of suppression subtractive hybridization and microarray analysis identified a large set of tomato genes that are induced or repressed during the response of resistant plants to avirulent XcvT3 bacteria. These genes were grouped in clusters based on coordinate expression kinetics, and classified into over 20 functional classes. Among them we identified genes that are directly modulated by expression of the type III effector protein AvrXv3 and genes that are induced also during the tomato resistance response to Pseudomonas syringae pv. tomato. 2. Characterization of molecular and biochemical properties of the tomato LeMPK3MAP kinase. A detailed molecular and biochemical analysis was performed for LeMPK3 MAP kinase, which was among the genes induced by XcvT3 in resistant tomato plants. LeMPK3 was induced at the mRNA level by different pathogens, elicitors, and wounding, but not by defense-related plant hormones. Moreover, an induction of LeMPK3 kinase activity was observed in resistant tomato plants upon Xcvinfection. LeMPK3 was biochemically defined as a dual-specificity MAP kinase, and extensively characterized in vitro in terms of kinase activity, sites and mechanism of autophosphorylation, divalent cation preference, Kₘand Vₘₐₓ values for ATP. 3. Characteriztion of molecular properties of the Xcveffector protein AvrRxv. The avirulence gene avrRxvis involved in the genetic interaction that determines tomato resistance to Xcvrace T1. We found that AvrRxv functions inside the plant cell, localizes to the cytoplasm, and is sufficient to confer avirulence to virulent Xcvstrains. In addition, we showed that the AvrRxv cysteine protease catalytic core is essential for host recognition. Finally, insights into cellular processes activated by AvrRxv expression in resistant plants were obtained by microarray analysis of 8,600 tomato genes. Scientific and agricultural significance: The findings of these activities depict a comprehensive and detailed picture of cellular processes taking place during the onset of tomato resistance to Xcv. In this research, a large pool of genes, which may be involved in the control and execution of plant defense responses, was identified and the stage is set for the dissection of signaling pathways specifically triggered by Xcv.
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Sessa, Guido, e Gregory Martin. Role of GRAS Transcription Factors in Tomato Disease Resistance and Basal Defense. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696520.bard.
Texto completo da fonteResumo:
The research problem: Bacterial spot and bacterial speck diseases of tomato are causedby strains of Xanthomonas campestris pv. vesicatoria (Xcv) and Pseudomonas syringae pv.tomato (Pst), respectively. These bacteria colonize aerial parts of the plant and causesignificant losses in tomato production worldwide. Protection against Xcv and Pst bycultural practices or chemical control has been unsuccessful and there are only limitedsources of genetic resistance to these pathogens. In previous research supported in part byBARD IS-3237-01, we extensively characterized changes in tomato gene expression uponthe onset of spot and speck disease resistance. A remarkable finding of these studies wasthe inducibility in tomato leaves by both Xcv and Pst strains of genes encodingtranscriptional activator of the GRAS family, which has not been previously linked todisease resistance. Goals: Central goals of this research were to investigate the role of GRAS genes in tomatoinnate immunity and to assess their potential use for disease control.Specific objectives were to: 1. Identify GRAS genes that are induced in tomato during thedefense response and analyze their role in disease resistance by loss-of-function experiments.2. Overexpress GRAS genes in tomato and characterize plants for possible broad-spectrumresistance. 3. Identify genes whose transcription is regulated by GRAS family. Our main achievements during this research program are in three major areas:1. Identification of tomato GRAS family members induced in defense responses andanalysis of their role in disease resistance. Genes encoding tomato GRAS family memberswere retrieved from databases and analyzed for their inducibility by Pst avirulent bacteria.Real-time RT-PCR analysis revealed that six SlGRAS transcripts are induced during theonset of disease resistance to Pst. Further expression analysis of two selected GRAS genesshowed that they accumulate in tomato plants in response to different avirulent bacteria orto the fungal elicitor EIX. In addition, eight SlGRAS genes, including the Pst-induciblefamily members, were induced by mechanical stress in part in a jasmonic acid-dependentmanner. Remarkably, SlGRAS6 gene was found to be required for tomato resistance to Pstin virus-induced gene silencing (VIGS) experiments.2. Molecular analysis of pathogen-induced GRAS transcriptional activators. In aheterologous yeast system, Pst-inducible GRAS genes were shown to have the ability toactivate transcription in agreement with their putative function of transcription factors. Inaddition, deletion analysis demonstrated that short sequences at the amino-terminus ofSlGRAS2, SlGRAS4 and SlGRAS6 are sufficient for transcriptional activation. Finally,defense-related SlGRAS proteins were found to localize to the cell nucleus. 3. Disease resistance and expression profiles of transgenic plants overexpressing SlGRASgenes. Transgenic plants overexpressing SlGRAS3 or SlGRAS6 were generated. Diseasesusceptibility tests revealed that these plants are not more resistant to Pst than wild-typeplants. Gene expression profiles of the overexpressing plants identified putative direct orindirect target genes regulated by SlGRAS3 and SlGRAS6. Scientific and agricultural significance: Our research activities established a novel linkbetween the GRAS family of transcription factors, plant disease resistance and mechanicalstress response. SlGRAS6 was found to be required for disease resistance to Pstsuggesting that this and possibly other GRAS family members are involved in thetranscriptional reprogramming that takes place during the onset of disease resistance.Their nuclear localization and transcriptional activation ability support their proposed roleas transcription factors or co-activators. However, the potential of utilizing GRAS familymembers for the improvement of plant disease resistance in agriculture has yet to bedemonstrated.