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1

Uddin, Md Nizam. "Heterosis in wheat (Triticum aestivum L.)". Thesis, The University of Sydney, 1991. https://hdl.handle.net/2123/26310.

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Three field experiments were established to provide information upon which strategic decisions could be made on the advisability of breeding hybrid wheat versus pure lines for Australian wheat growing conditions. These experiments would determine the level and stability of heterosis for grain yield, provide data on how hybrids obtained their yield, indicate the accuracy of different planting arrangements for measuring heterosis and compare the performance of pure lines derived from heterotic hybrids with the F1 hybrids. Six hand-made, two produced using a chemical gametocide and two commercial hybrids were tested along with their parents (except for the commercial hybrids for which the parents were unavailable) in replicated hill plots in three environments (sowing date s) for two seasons, 1987 and 1988. The hybrids produced significantly more grain yield in all six environments and were marginally more stable than their parents. The best hybrid outyielded the commercial cultivar by 18.2 %. Mea n protein content of the hybrids was marginally lower r than that for the parents, i. e., 14.4 % compared with 14.8 %. Howe ver, the three high est yielding hybrids, despite their 14.5 —18. 3% yield advantage over the best yielding check cultivar were high er in protein than it by as much as 1.2% protein. Kernel weight appeared to contribute most consistently to yield heterosis. All hybrids were taller than the mid-parent value and half of them were taller than the high-parent. However, the increased height did not affect their harvest indices adversely. With only one exception the hybrids had significantly higher harvest indices compared with the mid-parent value. Eight of the hybrids from the above experiment were tested in hill plots, space-planted plots and in multiple row plots (large plots) planted at a seeding density comparable with commercial practice Genotype means from the three plot types for grain yield. Its components, plant height and harvest index were positively and significantly correlated. The three plot types determined genotypic differences with similar precision. Similar levels of heterosis were observed in hill plots and space-planted plots. 22.1 and 22.9%, respectively, whereas a relatively lower level was observed in large plots (11.7%). This reduction was mainly due to reduced heterosis for tiller number. The potential to develop pure lines with similar yield to the heterotic F1 hybrids was investigated using four hand-made hybrids and the commercial hybrid, Comet. Seventy five single seed descent derived F- (F6 in the case of Comet) lines were compared with their parents (except for Comet) and the F1 hybrid in replicated hill plots. Significant high-parent heterosis up to 15.1% for grain yield was observed for the hand-made hybrids. Estimation of heterosis for Comet was not possible because of the unavailability of its parents. All crosses produced pure lines which yielded similarly to their Fi hybrid indicating that pure lines of equivalent yield to the heterotic Fi could be developed by conventional breeding. There was no consistent pattern amongst the pure lines as to how they achieved their yield via their components. Optimal plant height was in the range of 70-90 cm, i.e., semidwarf types, for all crosses. The results of the experiments conducted indicate that although considerable and stable levels of heterosis were observed, pure lines of comparable yield to the hybrids could be produced. The decision to breed hybrids as opposed to breeding pure lines would appear therefore to depend on commercial rather than scientific considerations.
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2

Zainuddin. "Genetic transformation of wheat (Triticum aestivum L.)". Title page, Contents and Abstract only, 2000. http://web4.library.adelaide.edu.au/theses/09APSP/09apspz21.pdf.

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Bibliography: leaves 127-151. The successful application of genetic engineering in wheat is dependent on the availability of suitable tissue culture and transformation methods. The primary object of this project was the development of these technologies using elite Australian wheat varieties.
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3

Collin, François. "The tolerance of wheat (Triticum aestivum L.) to Septori tritici blotch". Thesis, University of Nottingham, 2018. http://eprints.nottingham.ac.uk/49156/.

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The Septoria tritici blotch disease (STB, pathogen Zymoseptoria tritici) is the most damaging foliar infection of wheat crops in Europe. Disease management strategies include cultivar resistance, disease escape strategy and fungicides. However, these strategies have failed to provide a complete protection of wheat crops. The STB tolerance is a complementary approach which aims to maintain yield in the presence of the symptoms. The tolerance of STB relies on plant physiology and source/sink balance: the sink demand (the grain growth) must be satisfied in spite of reduced source availability (photosynthetic capacity as affected by the STB symptoms on the leaves). The green canopy area, the senescence timing and the grain yield components are interesting potential sources of tolerance that were studied in this project. A data-mining study, one glasshouse experiment and two field experiments were carried out providing complementary insights on STB tolerance mechanisms. The genotype/environment interaction effects on tolerance traits were investigated for two seasons five locations/nine cultivars datasets. The nitrogen nutrition and metabolism of four doubled-haploid (DH) lines contrasting for STB tolerance were examined in a controlled glasshouse experiment at UMR ECOSYS (INRA,AgroParisTech) Grignon, France. The source/sink balance of six DH lines contrasting for STB tolerance was also examined according to their responses to a spikelet removal treatment, applied in a field experiment in Hereford, UK. Finally, a field experiment with two fungicide regimes (full disease control and non-target (STB) disease control) probed the STB tolerance of six modern UK winter wheat cultivars in Leicestershire, UK. The main objective was to verify identified potential STB tolerance traits in commercial cultivars. Putative STB tolerance traits have been identified such as the early heading date, the low degree of grain-source availability of healthy crops during the grain filling phase, the vertical canopy distribution favouring a relatively larger flag-leaf. Results showed these traits might be selectable in wheat breeding without a trade-off with the potential yield. Finally, the project also discussed the need for alternative STB tolerance quantification methods, as well as the importance of environmental variations which have to be taken into account to study genetic variation in tolerance, but which could also be used to discriminate tolerant environment.
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4

Al-Awami, Mussa Othman. "Spring wheat (Triticum aestivum) (L.)/scentless mayweed (Tripleurospermum inodorum) (L.), interactions". Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307628.

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5

Craufurd, P. Q. "Plant development and yield in wheat (Triticum aestivum L.)". Thesis, University of Reading, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.352335.

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6

Jenkinson, Peter. "Epidemiology of Fusarium in winter wheat (Triticum aestivum L.)". Thesis, Open University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386201.

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7

Hall, Sharon Anita. "Defence related lignin deposition in wheat (Triticum aestivum L.)". Thesis, University of Southampton, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.284581.

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8

Heyns, I. C. "Mapping of chromosome arm 7DL of Triticum aestivum L". Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/1584.

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Thesis (MSc (Genetics))--University of Stellenbosch, 2005.
The Russian wheat aphid, Diuraphis noxia (Mordvilko), is a serious insect pest of wheat and barley. It affects the quality and yield of grain by sucking plant sap from the newest growth whilst toxic substances are injected that destroy plant tissue. The Russian wheat aphid also acts as a vector of plant viruses. The cultivation of aphid resistant cultivars is the preferred control strategy and nine resistance genes, designated Dn1 to Dn9, have been identified. Another undesignated gene, Dnx, was found in the wheat accession PI220127. Mapping of the resistance genes relative to known markers will improve their use in breeding programs. The dominant RWA resistance gene, Dn5, was identified in the accession PI294994 and mapped to chromosome arm 7DL. However, recent reports have placed Dn5 on ...
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9

Salgado, Adliz Ayram de Bastos Budziak. "Efeito residual da aplicação de gesso na eficiência da adubação fosfatada para a sucessão trigo-soja em sistema plantio direto". Universidade Estadual de Ponta Grossa, 2017. http://tede2.uepg.br/jspui/handle/prefix/2562.

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Submitted by Eunice Novais (enovais@uepg.br) on 2018-07-05T17:43:12Z No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Adlliz Salgado.pdf: 1207262 bytes, checksum: 92b81817140d534259d580cdd9c338ff (MD5)
Made available in DSpace on 2018-07-05T17:43:12Z (GMT). No. of bitstreams: 2 license_rdf: 811 bytes, checksum: e39d27027a6cc9cb039ad269a5db8e34 (MD5) Adlliz Salgado.pdf: 1207262 bytes, checksum: 92b81817140d534259d580cdd9c338ff (MD5) Previous issue date: 2017-12-12
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
O P é o nutriente que mais limita a produtividade agrícola no Brasil, devido ao material de origem dos solos brasileiros, pela forte interação do P com o solo e pela sua precipitação com compostos de Fe, Al e Ca, por esse motivo os adubos fosfatados tem sido utilizado em maiores quantidades comprometendo as reservas mundiais que vem diminuindo. Com isso, há necessidade de se aprimorar a eficiência do uso de P na agricultura e o uso de gesso pode ser uma alternativa, devido à presença em sua composição de P e ao favorecimento do crescimento radicular. Sendo assim o objetivo deste trabalho foi avaliar o efeito residual da aplicação de gesso na eficiência da adubação fosfatada para a sucessão trigo-soja em sistema plantio direto. O experimento foi instalado em outubro de 2013, no município de Ponta Grossa, em um Latossolo Vermelho distrófico típico, textura argilosa. O delineamento experimental empregado foi o de blocos ao acaso, em parcelas subdivididas, com três repetições. Nas parcelas (180 m2) foram aplicadas, no sulco de semeadura, nas safras de inverno e verão, quatro doses de P (0, 30, 60 e 90 kg P2O5 ha-1) na forma de superfosfato triplo (SFT) e, nas subparcelas (45 m2) foram empregadas quatro doses de gesso agrícola (0, 2, 4, 6 t ha-1), em outubro de 2013. A sucessão de culturas do experimento foi: trigo (2015) e soja (2015/2016), sendo avaliado o efeito residual de gesso agrícola após 33 meses de sua aplicação (outubro de 2013) e os efeitos das doses de P. As avaliações realizadas foram os atributos químicos do solo (pH, Al3+, Ca2+, Mg2+, K+, S-SO42- e P) nas camadas (0-10, 10-20, 20-40 e 40-60 cm de profundidade), e nas culturas do trigo (2015) e soja (2015/2016) foram avaliados: (diagnose foliar, extração, rendimento de grãos e o fator parcial de produtividade). O efeito residual de gesso agrícola na superfície e a adubação fosfatada no sulco de semeadura ocasionaram melhoria nos atributos químicos do solo, sendo que o incremento das doses de P ocasionaram aumento de P (0-20 cm), SO42- (20-40 cm), Ca2+ e K+ (40-60 cm) e diminuição de P (20-40 cm), SO42- (0-10 cm) e Mg2+ (40-60 cm). O efeito residual do gesso agrícola ocasionou aumento de Ca2+ (0-60 cm), P (0-20 cm), SO42- (10-60 cm) e K+ (40-60 cm), e diminuição de Al3+ (10-20 cm). Na cultura do trigo, o incremento das doses de P aumentaram o teor foliar de P, Ca e S e diminuiu a extração de Fe, já com o incremento de gesso ocorreu aumento do teor foliar de Ca e S e diminuição do teor de Mg, e aumento da extração de P, S e Cu. Na cultura da soja o incremento das doses de P e de gesso não ocasionaram diferenças estatísticas na diagnose da cultura, já para a extração da planta, houve aumento da extração de K, Fe, Mn e Zn com o incremento das doses de P, e aumento na extração de P, Ca, S e Fe com o incremento das doses de gesso. Para o rendimento de grãos o aumento das doses de P não foram eficientes para aumentar a produtividade das culturas de trigo e soja, já o incremento das doses de gesso foi eficiente somente para a cultura do trigo aumentando em 21,8% o rendimento de grãos. O incremento nas doses de P aplicadas aumentou o fator parcial de produtividade de P (FPPP) nas culturas de trigo e soja, quando a menor dose de P (30 kg ha-1) foi aplicada. O efeito residual do gesso no aumento da produtividade de trigo não foi ocasionado por melhoria na eficiência de utilização de P pelas plantas.
P is the nutrient, which most limits agricultural productivity in Brazil, due to the material from Brazilian soils, because of the strong interaction of P with soil, and its precipitation with compounds Fe, Al and Ca, for this reason, phosphate fertilization has been utilized in larger quantities, compromising world supplies, which have been diminishing. Therefore, it is necessary to improve the efficiency of the utilization of P in agriculture, and the utilization of gypsum may be an alternative, due to the presence of P in its composition and the favoring of root growth. Thus, this paper is aimed at evaluating residual effect of gypsum application in the efficiency of phosphate fertilization for the succession wheat-soybean in no tillage system. The experiment was installed in October 2013, in the municipality of Ponta Grossa, in a typical dystrophic Red Latosol, clay texture. The experimental delineation applied was the blocks by chance, in subdivided parcels, with three repetitions. In the (180 m2) parcels, four doses of P (0, 30, 60 and 90 kg P2O5 ha-1) were applied, in-furrow, for winter and summer harvests, in the form of Triple SuperPhosphate (TSP) and, in the (45 m2) subparcels, four doses of agricultural gypsum were applied (0, 2, 4, 6 t ha-1), in October of 2013. The succession of crops for the experiment was: wheat (2015) and soybean (2015/2016), residual effect of agricultural gypsum was evaluated 33 months after its application (October of 2013), along with the effects of P doses. The evaluations performed were about the chemical attributes of the soil (pH, Al3+, Ca2+, Mg2+, K+, S-SO42- and P) in layers (0-10, 10-20, 20-40 and 40-60 cm deep) in wheat crops (2015), and in soybean crops (2015/2016); foliar diagnosis, extraction, grain yield, and the partial factor productivity were evaluated. The residual effect of agricultural gypsum in the surface and phosphate fertilization in-furrow caused improvements to the chemical attributes of the soil, where the addition of P doses caused an increase of P (0-20 cm), SO42- (20-40 cm), Ca2+ and K+ (40-60 cm) and a decrease of P (20-40 cm), SO42- (0-10 cm) and Mg2+ (40-60 cm). The residual effect of agricultural gypsum caused an increase of Ca2+ (0-60 cm), P (0-20 cm), SO42- (10-60 cm) and K+ (40-60 cm), and a decrease of Al3+ (10-20 cm). In wheat crops, the addition of P doses increased the foliar content of P, Ca and S, and decreased the extraction of Fe, on the other hand with the addition of gypsum an increase of Ca and S foliar content occurred and a decrease of Mg content, and an increase of P, S and Cu extraction. In soybean crops, the addition of P doses and gypsum caused statistical differences in the crop diagnosis, and then again, for the extraction of the plant, there was an increase of K, Fe, Mn and Zn extraction with the addition of P doses, and an increase of P, Ca, S and Fe extraction with the addition of gypsum doses. For grain yield, the increase of P doses were not efficient to boost productivity in wheat and soybean crops, as for the addition of gypsum doses, it was only efficient for wheat crops with a 21,8% increase of grain yield. The addition in applied P doses increased the partial factor productivity (PFP) of P in wheat and soybean crops, when the lowest dose of P (30 kg ha-1) was applied. The residual effect of gypsum in the increase of wheat productivity did not happen because of improvements in the efficiency of P usage by plants.
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10

Taylor, Victoria Louise. "The activities of herbicide safeners in wheat (Triticum aestivum L.)". Thesis, Durham University, 2012. http://etheses.dur.ac.uk/3926/.

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Commercialised herbicide safeners (also known as protectants or antidotes) are synthetic chemicals used to enhance herbicide tolerance in cereal crops. They do this by causing an up-regulation in xenobiotic detoxifying enzymes such as glutathione transferases (GSTs). Seedlings of wheat (Triticum aestivum cv ‘Einstein’) were sprayed with the safeners cloquintocet mexyl, fenchlorazole ethyl and mefenpyr diethyl. All three compounds caused an identical up regulation of GSTs from the phi, tau and lambda classes, despite their differences in chemistry. Using cloquintocet mexyl as a classic wheat safener treatment, it was found that GST induction was both dose and time dependent. Safening was found to be associated with the rapid hydrolysis of the parent ester to cloquintocet acid. When the free acid was tested, the GST-induction response obtained was identical to that determined with the parent ester, suggesting that cloquintocet itself is the active safener. GST induction was found to be tissue specific within the wheat shoots, with the lambda GSTs being preferentially expressed in the meristematic tissue. Proteomic 2 D gel analysis revealed that the tau TaGSTU3 was a major up-regulated GST. In addition, six GSTs that were previously shown in literature to be up-regulated by herbicide safeners in wheat were cloned, expressed and characterized as the respective recombinant enzymes and renamed to bring them in line with existing nomenclature. The GSTs cloned included TaGSTU3, TaGSTU6, TaGSTF4, TaGSTF10 and TaGSTL1. Metabolism studies showed that following the hydrolysis of cloquintocet mexyl, no further down- stream metabolites could be identified and none of the up-regulated GSTs showed any activity toward the safener. However TaGSTU3 was found to bind and be inhibited by cloquintocet free acid as determined by isothermal titration calorimetry. Safener treatment also led to a transient inhibition of GST activity in crude wheat extracts after spraying the seedlings. In addition to the induction of GSTs, safener treatments also resulted in an enhanced growth of wheat seedlings. The work presented in this thesis confirms that very different compounds can induce apparently identical downstream events at the level of GST enhancement and that these induction events underpin wider changes in plant physiology.
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11

McCallum, John Allan. "Biochemistry of phenolic compounds in wheat grain (Triticum aestivum L.)". Thesis, University of Canterbury. Botany, 1989. http://hdl.handle.net/10092/5723.

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Phenolic content, o-diphenol oxidase activity, flour colour and bread crumb colour have been examined for flour streams from a commercial flour mill and in flours and wholemeals of a number of New Zealand wheat cultivars. Phenolic content and o-diphenol oxidase activity varied significantly both between milling flour streams and between cultivars. Flour and bread crumb colour correlated significantly with phenolic content and o-diphenol oxidase activity in the milling flour streams. A similar relationship was also observed between o-diphenol oxidase activity and the colour of flour and bread prepared from several different cultivars. These observations are consistent with an involvement of o-diphenol oxidase and phenolics in the pigmentation of both wheat flour and bread. Commercial wheat bran has been found to contain low levels (<50 µg.g-1) of (+)catechin and soluble proanthocyanidins soluble in aqueous acetone. Detection of these compounds in crude extracts by conventional methods was complicated by the presence of methoxyhydroquinone glycosides and other interfering substances. Chromatographic studies indicated that, in addition to several dimeric proanthocyanidins, bran contains trimeric and/or other oligomeric proanthocyanidins. The oligomeric proanthocyanidins contained mostly prodelphinidin and some procyanidin units, whilst the dimeric proanthocyanidins may also contain some propelargonidin units. Two dimeric proanthocyanidins were isolated and tentatively characterized as catechin-(4α→)-catechin (procyanidin B3) and gallocatechin (4α→8)-catechin (prodelphinidin B3). These observations suggest that the flavanol content of wheat grain may be qualitatively similar to that of barley, although quantitatively much smaller. The identification of these compounds in mature wheat grain confirmed earlier reports of their presence in immature grain and supports the hypothesis that they contribute to seedcoat pigmentation. Changes in soluble phenolics and hydroxycinnamic acids and the activities of L-phenylalanine ammonia-lyase (PAL, EC No. 4.3.1.5) and chalcone-flavanone isomerase (CHI E.C. 5.5.1.6) were examined during the development and maturation of several spring bread wheat cultivars. Activities of both PAL and CHI were high in the early milk stage of grain development and this activity was located principally in the testa and pericarp tissues. Comparison of enzyme activities at this stage indicated significant differences between red- and white-grained cultivars. These different patterns of activity also correlated with significant differences in germinability at maturity. It is suggested that the common association between red seedcoat pigmentation and dormancy may be related to differences in the regulation of phenolic biosynthesis in the immature seedcoat. Studies of the tissue distribution of enzyme activities suggested that phenolic biosynthetic activities were higher in the embryo during the dough stage of grain development. Chromatographic studies suggested that there were significant qualitative and quantitative changes in phenolic content during grain development and maturation. Changes in ferulic and other hydroxycinnamic acids indicated that these were subject to considerable turnover and metabolism during grain development.
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12

Triggs, Heidi M. "Haploid production and genetic transformation of wheat (Triticum aestivum L.)". Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243689.

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13

Vaux, P. "Ribonucleotide content of wheat embryos (Triticum aestivum L.) during imbibition". Thesis, University of Manchester, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.378808.

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14

Verma, Vinesh. "Genetic analysis of agronomic traits in wheat (Triticum aestivum L.)". Thesis, University of Reading, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270848.

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15

Noori, Seyed Ahmad Sadat. "Salinity tolerance in wheat (Triticum aestivum L.) and its relatives". Thesis, University of Liverpool, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367305.

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16

Mohamed, Abulgasem Besheir. "Genetic basis of salt tolerance in wheat triticum aestivum (L)". Thesis, University of Liverpool, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337214.

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17

Steinmeyer, Frederick Thomas. "Spike temperature depression of wheat (Triticum aestivum L.) at anthesis". Thesis, University of Reading, 2016. http://centaur.reading.ac.uk/68662/.

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Global climate change has been forecast to result in significant alterations to current temperature and precipitation patterns in cereal growing regions worldwide. The increased occurrence of elevated temperature stress at anthesis is likely to result in significant yield losses in wheat (Triticum aestivum L.). The ability of plant tissue to depress its temperature has been reported in relation to the canopy but recent work has demonstrated that spike temperature depression (STD) can be assessed in controlled environments (CE). The findings from two consecutive years of potbased CE experiments, a field-based experiment under polytunnel cover and a rhizobox-based experiment, in which the thermal dynamics of the spike and flag leaf under contrasting conditions of elevated temperature and water-deficit stress at anthesis, are reported. Flag leaf temperature depression (FLTD) was significantly greater than STD at anthesis. The data do not demonstrate an increased cooling capacity of the spike in the early stages of anthesis but rather in the latter stages, a phenomenon hypothesized to be primarily associated with the onset of senescence in the canopy. The inconsistent relationship observed between FLTD/STD at anthesis and grain yield (GY) does not currently elucidate whether a failure to depress tissue temperature at anthesis is associated with a yield penalty. The effect of experimental design on the physiological response to abiotic stress at anthesis was explored. The plant-wide distribution of photoassimilates at mid-anthesis was examined. Starch and water-soluble carbohydrate content in the flag leaf, peduncle and glumes was not found to correlate to GY. Further examination of the effects that abiotic stress at anthesis have on the photoassimilate distribution and GY need to take place in field-grown wheat.
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18

Goodwin, Hazel Emma. "The evolution and spread of Triticum aestivum L. in Europe". Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.702440.

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19

Cartelat, Aurélie. "Etude de l'autofluorescence des feuilles de blé (triticum aestivum L. )". Paris 6, 2004. http://www.theses.fr/2004PA066444.

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20

Livesey, Nancy L. "Towards the haploid production of transgenic wheat (Triticum aestivum L.)". Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395623.

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21

Collin, François. "La tolérance du blé (Triticum aestivum L.) à la Septoriose". Thesis, Paris, Institut agronomique, vétérinaire et forestier de France, 2017. http://www.theses.fr/2017IAVF0028/document.

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La septoriose (pathogène Zymoseptoria tritici) est la plus importante maladie foliaire des cultures de blé en Europe. Les méthodes de lutte comprennent la résistance variétale, les stratégies d’évitement de la maladie et le recours aux fongicides. Cependant, ces stratégies n’assurent pas une protection complète des cultures de blé. La tolérance à la septoriose est une approche complémentaire qui vise justement à maintenir le rendement en présence de symptômes. La tolérance à la septoriose dépend de traits physiologiques de la plante et d’équilibres source/puits : la demande des puits (croissance des grains) doit être satisfaite malgré une disponibilité réduite des sources (capacité photosynthétique réduite par les symptômes foliaires). La surface verte du couvert, la sénescence et les composantes du rendement sont des traits potentiels de tolérance intéressants qui ont été étudiés lors de ce projet. Une étude de datamining, une expérience en serre et deux expériences au champ ont été menées pour fournir des informations complémentaires sur les mécanismes de tolérance à la septoriose. Les effets des interactions génotype × environnement sur les traits de tolérance ont été étudiés pour deux saisons × cinq localisations × neuf cultivars. La nutrition azotée et le métabolisme de quatre lignées double-haploïdes (DH, contrastées du point de vue de leur tolérance à la septoriose) ont été examinés dans une expérience en conditions contrôlées à l'UMR ECOSYS (INRA, AgroParisTech Grignon, France). Les bilans source/puits de six lignées DH contrastant pour la tolérance ont également été examinés en fonction de leurs réponses à un traitement d'égrainage, appliqué dans une expérience au champ à Hereford (Royaume-Uni). Enfin, une expérience au champ avec deux stratégies fongicides (contrôle total des maladies / lutte contre les maladies non-ciblées) a permis d’étudier la tolérance à la septoriose de six cultivars modernes (Leicestershire, Royaume-Uni). L'objectif principal était de vérifier les traits potentiels de tolérance à la septoriose sur des cultivars actuellement commercialisés. Des traits potentiels de tolérance à la septoriose ont été identifiés tels que la date d’épiaison, le faible degré de limitation des puits par les sources lors de la phase de remplissage du grain des couverts sains, la distribution verticale des surfaces foliaires favorisant des feuilles supérieures relativement grandes. Les résultats ont montré que ces caractères pourraient être sélectionnables, sans compromis avec le rendement potentiel. Enfin, le projet a également discuté du besoin de méthodes alternatives de quantification de la tolérance du blé à la septoriose, ainsi que de l'importance des variations environnementales qui doivent être prises en compte pour étudier les variations génétiques de la tolérance, mais qui pourraient également être utilisées pour identifier des environnements tolérants
The Septoria tritici blotch disease (STB, pathogen Zymoseptoria tritici) is the most damaging foliar infection of wheat crops in Europe. Disease management strategies include cultivar resistance, disease escape strategy and fungicides. However, these strategies have failed to provide a complete protection of wheat crops. The STB tolerance is a complementary approach which aims to maintain yield in the presence of the symptoms. The tolerance of STB relies on plant physiology and source/sink balance: the sink demand (the grain growth) must be satisfied in spite of reduced source availability (photosynthetic capacity as affected by the STB symptoms on the leaves). The green canopy area, the senescence timing and the grain yield components are interesting potential sources of tolerance that were studied in this project. A data-mining study, one glasshouse experiment and two field experiments were carried out providing complementary insights on STB tolerance mechanisms. The genotype × environment interaction effects on tolerance traits were investigated for two seasons × five locations × nine cultivars datasets. The nitrogen nutrition and metabolism of four doubled-haploid (DH) lines contrasting for STB tolerance were examined in a controlled-glasshouse experiment at UMR ECOSYS (INRA,AgroParisTech) Grignon, France. The source/sink balance of six DH lines contrasting for STB tolerance was also examined according to their responses to a spikelet removal treatment, applied in a field experiment in Hereford, UK. Finally, a field experiment with two fungicide regimes (full disease control and non-target (STB) disease control) probed the STB tolerance of six modern UK winter wheat cultivars in Leicestershire, UK. The main objective was to verify identified potential STB tolerance traits in commercial cultivars. Putative STB tolerance traits have been identified such as the early heading date, the low degree of grain-source limitation of healthy crops during the grain filling phase, the vertical canopy distribution favouring a relatively larger flag-leaf. Results showed these traits might be selectable in wheat breeding without a trade-off with the potential yield. Finally, the project also discussed the need for alternative STB tolerance quantification methods, as well as the importance of environmental variations which have to be taken into account to study genetic variation in tolerance, but which could also be used to discriminate tolerant environment
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22

Girma, Kefyalew. "Interference of wild mustard, Sinapis arvenis L., in spring wheat, Triticum aestivum L". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ31832.pdf.

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Dezfooli, Amin. "Competition between wild mustard (Sinapis arvensis L.) and spring wheat (Triticum aestivum L.)". Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0027/MQ51059.pdf.

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24

Radic-Miehle, Helga. "Charakterisierung von Speicherproteinen und Speicherprotein-Genen des Dinkels (Triticum spelta L.) sowie Abgrenzung zum hexaploiden Weizen (Triticum aestivum L.) /". Stuttgart : Grauer, 2000. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=009152501&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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25

Malik, Iram. "The effects of ozone and salinity on wheat (Triticum aestivum L.)". Thesis, Imperial College London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343901.

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Bhutto, Liaquat Ali. "Understanding the mechanisms of drought tolerance in wheat (Triticum aestivum L.)". Thesis, University of Sheffield, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.522505.

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Semikhodskii, Andrei G. "Mapping quantitative traits for salinity responses in wheat (Triticum aestivum L.)". Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302054.

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28

Lisiecki, Karol. "Reakcja genotypów pszenicy (Triticum aestivum L.) na patogeny z rodzaju Rhizoctonia". Rozprawa doktorska, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, 2021. http://dlibra.utp.edu.pl/Content/3620.

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Celem pracy było uzyskanie profilów biologicznych genotypów pszenicy w kontekście reakcji oraz ewentualnej obrony przeciwko patogenom z rodzaju Rhizoctonia. Wykonana analiza składowych głównych (PCA) wykazała zróżnicowanie uruchamianych mechanizmów obronnych w zależności od genotypu patogena. W efekcie badań polowych i laboratoryjnych wyodrębniono grupy genotypów roślin o różnych poziomach podatności na działanie grzybów z rodzaju Rhizoctonia
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29

Assmann, Isidoro Carlos. "Diversidade genética e análise dialélica em trigo (Triticum aestivum (L.) Thell)". Universidade Federal de Viçosa, 1997. http://www.locus.ufv.br/handle/123456789/10250.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico
Nove genótipos de trigo (Anahuac, BH 1146, Trigo BR 10 - Formosa, Trigo BR 26 - São Gotardo, CEP 24 - industrial, EMBRAPA 15, EMBRAPA 16, EMBRAPA 21 e EMBRAPA 22), seus F 1 e recíprocos, constituindo um dialelo completo, foram plantados, em maio de 1995, no campo Experimental do Setor de Agronomia da UFV. A parcela constituiu de duas linhas de 1,0 m, espaçadas por 0,30 m entre linhas e 0,10 m entre plantas. As características avaliadas foram: ciclo (do plantio ao espigamento); altura da planta; número de espigas por planta; número de espiguetas estéreis por planta e por espiga; número de espiguetas férteis por planta e por espiga; número de grãos por planta, por espiga e por espigueta; peso de grãos por planta e de mil grãos; e produtividade por parcela e por hectare. A diversidade genética dos genótipos foi avaliada pela distância generalizada de Mahalanobis, com os genótipos Anahuac e Trigo BR 26 - São Gotardo apresentando a menor distância e os genótipos BH 1146 e Trigo BR 26 - São Gotardo, a maior distância. Pelos agrupamentos, segundo os métodos do vizinho mais próximo e de Tocher, foi possível formar quatro grupos. Os genótipos de trigo irrigado (Anahuac, Trigo BR 10 - Formosa e Trigo BR 26 - São Gotardo) formaram um grupo, enquanto o outro genótipo de trigo irrigado (EMBRAPA 22) formou um grupo isoladamente. Os genótipos mais altos (BH 1146 e CEP 24 - Industrial) formaram um outro grupo, enquanto os genótipos de trigo de estatura intermediária (EMBRAPA 15, EMBRAPA 16 e EMBRAPA 21) formaram o quarto grupo. A dispersão gráfica dos genótipos, assim como suas distâncias, foram apresentadas pela metodologia de Singh (1981). A altura da planta explicou mais de 92% da variabilidade genética existente entre os genitores. Para a análise dialélica, utilizaram-se as metodologias de Hayman (1954) e Griffing (1956). Os efeitos da capacidade geral de combinação foram maiores que os da capacidade específica e os recíprocos, para os caracteres estudados. Os genótipos BH 1146, Trigo BR 26 - São Gotardo e EMBRAPA 22 apresentaram os maiores efeitos positivos da capacidade geral de combinação. Para a maioria dos caracteres estudados, o efeito genético aditivo não foi o mais importante, segundo a metodologia de Hayman (1954). A herança dos caracteres estudados mostrou ser oligogênica.
Nine genotypes of wheat (Anahuac, BH 1146, BR 10 – Formosa, Trigo BR 26 – São Gotardo, CEP 24 – Industrial, EMBRAPA 15, EMBRAPA 16, EMBRAPA 21 e EMBRAPA 22) their F 1 and reciprocals, constituting a complete diallel, were planted on May 1992, on the experimental field of the Agronomic Section of the UFV, Minas Gerais State, Brazil. The plots in the blocks were of 2 lines with 1.0 m, spaced at 0.30 m among lines and 0.10 m among plants. The characters evaluated were: cycle (from planting to hearing), plant height, and number of spikes by plant, number of sterile spikelets by spike and by plant, number of fertile spikelets by spike and by plant, number of grains by plant, by spike; and by spikelet, weight of grains by plant, and of a thousond grains, and productivity by hectere. The genetic diversity of the genotypes were evaluated by the Generalized Distance of Mahalanobis, and the genotypes Anahuac and Trigo BR 26 – São Gotardo presented the shortest distance and the genotypes BH 1146 and Trigo BR 26 – São Gotardo presented the greatest distance. According to the grouping analysis using the closest neighbor method and the Tocher method, it was possible to form four groups. The irrigated wheat genotypes (Anahuac, Trigo BR 10 – Formosa and BR 26 – São Gotardo) formed a group, while the irrigated wheat genotype (EMBRAPA 22) formed a single group. The genotypes with the higher plants (BH 1146 and CEP 24 – Industrial) formed another group and the genotypes of wheat of intermediary height (EMBRAPA 15, EMBRAPA 16 and EMBRAPA 21) formed the fourth and bot group. The graphic dispersion of the genotypes, as well as their distances, were presented according to Singh (1981) methodology. The plant hight explained more than 92% of the genetic variability of the parent plants. For the diallelic analyhsis, the methodologies of Griffing (1956) and Hayman (1954) were used. The general capacity effects of combination were greater than those of the specific capacity, and of the reciprocal, for the studied characters. The genotypes BH 1146, Trigo BR 26 – São Gotardo and EMBRAPA 22 presented the greatest positive effects of the general capacity of combination. For almost all of the studied characters, tha additive genetic effect was not the most important one, according to the methodology of Hayman (1954). The heritage of the studied characters showed to be oligogenic.
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30

Parrott, David L. Jr. "Physiological and Biochemical Aspects of Agrobacterium-Wheat (Triticum Aestivum L.) Interactions". DigitalCommons@USU, 2003. https://digitalcommons.usu.edu/etd/1346.

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Agrobacterium tumefaciens and A. rhizogenes are the causal agents of gall or hairy root disease, but normally the bacteria do not cause disease in wheat. However, both bacteria grew without inhibition when exposed to intact or wounded wheat roots or embryos, and they colonized wheat root surfaces to levels similar to dicotyledonous plants. A. tumefaciens and A. rhizogenes induced 23% cell death after a 1-h exposure to wheat embryo cells grown in 7.4 mM O2, while the extent of cell death at 2.1 mM 02 was 8%. Contact with A. tumefaciens or A. rhizogenes caused cultured wheat embryo and root cells to rapidly produce H202, which decreased when embryos and roots were cultured at 2.1 mM O2. Browning and autofluorescence, and an increase in ferulic acid in cell walls, were observed in wheat embryo and root epidermal cells exposed to Agrobacterium, but . neither lignin nor callose was detected. Agrobacterium appeared to induce resistance-like responses in wheat that may limit transformation efficiency. The inability to regenerate wheat plants using tissue culture has been a limitation to high efficiency transformation. Regeneration via somatic embryogenesis was improved significantly by simulating the in ovulo environment to which the immature wheat embryos are exposed. Triticum embryo culture medium (TEC) improved callus formation, somatic embryo formation, and regeneration from somatic embryos while reducing precocious germination when compared to growth on Murashige and Skoog medium. Regeneration frequencies were improved when embryos were cultured at the O2 concentration found in the wheat ovule (2.1 mM O2) rather than atmospheric 02concentration (7.4 mM O2). Agrobacterium-mediated transformation of wheat was limited by tissue necrosis following co-cultivation, and by poor plant regeneration. Reduction of necrosis and increased plant regeneration were accomplished by amending the culture medium with antioxidant compounds and by reducing the O2 tension in which the wheat embryos were cu1tured. Twelve days past anthesis (DPA), wheat embryos were co-cultivated with Agrobacterium tumefaciens strains WAg 11 or EHA 101, incubated on TEC medium containing antioxidant compounds (catalase, cysteine and ascorbic acid), and cultured at 2.1 mM O2 concentrations. Transformation was documented in 6.0% ofregeneratedA. tumefaciens WAg 11 exposed wheat plants using the firefly luciferase (luc) reporter system.
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31

Michard, Robin. "Identification des facteurs déterminant le ciblage de la recombinaison méiotique chez le blé tendre (Triticum aestivum L.)". Thesis, Université Clermont Auvergne‎ (2017-2020), 2019. http://www.theses.fr/2019CLFAC023/document.

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La compréhension des mécanismes régissant la recombinaison méiotique chez le blé tendre (Triticum aestivum L.) devient essentielle puisqu’elle est le levier principal utilisé par les sélectionneurs pour le brassage génétique et obtenir de nouvelles variétés élites comportant des introgressions de régions d’intérêt provenant de ressources génétiques exotiques. A cette fin, l’utilisation chez le blé tendre d’une nouvelle biotechnologie de ciblage de la recombinaison méiotique développée chez la levure par la société Meiogenix semble être prometteuse. Cette biotechnologie, nommée SpiX, fait intervenir un domaine protéique de liaison à l’ADN couplé à la protéine SPO11 responsable des cassures double-brins de l’ADN, initiatrices de la recombinaison méiotique ou crossovers (CO). Le développement d’une nouvelle technique de conservation des embryons immatures a permis d’améliorer les conditions de transformation par biolistique du blé tendre pour l’application de la technologie SpiX. L’exploitation de la séquence du génome du blé a permis d’isoler les gènes codant pour les protéines SPO11 du blé tendre. La complémentation hétérologue inédite de mutants pour les protéines SPO11 d’Arabidopsis thaliana avec les orthologues ainsi découverts chez le blé tendre montre leur grande conservation de séquence et de fonction au sein des plantes et leur potentielle fonctionnalité pour la biotechnologie SpiX. Enfin le test de différents domaines de liaison à l’ADN et de différentes cibles le long du chromosome 3B de blé tendre montre que la biotechnologie SpiX requiert des ajustements en fonction de l’espèce chez laquelle celle-ci doit fonctionner. Ces résultats sont ainsi l’opportunité de lever un premier voile sur le ciblage de la recombinaison méiotique chez une espèce de grande culture et de mieux comprendre les mécanismes de détermination des sites de cassures double-brins initiatrices de la recombinaison méiotique chez le blé tendre
Understanding the mechanisms governing meiotic recombination in bread wheat (Triticum aestivum L.) is essential since it is the main tool used by breeders for genetic admixing and obtaining new elite varieties with introgression of regions of interest from exotic genetic resources. To this end, the use in bread wheat of a new biotechnology targeting meiotic recombination developed in yeast by Meiogenix seems to be promising. This biotechnology, named SpiX, involves a DNA-binding domain fused to the SPO11 protein responsible for DNA double-strand breaks, initiating meiotic recombination or crossovers (CO). The development of a new conservation protocol for wheat immature embryos has improved the conditions for bread wheat transformation through biolistic, and thus for the application of SpiX technology. The exploitation of the wheat genome sequence made it possible to isolate the bread wheat genes for SPO11 proteins. A novel heterologous complementation of Arabidopsis thaliana mutants for SPO11s with the bread wheat orthologous freshly discovered shows their great conservation of sequence and function within plants and their potential functionality for SpiX biotechnology. Finally, the testing of different DNA-binding domains and different targets along bread wheat 3B chromosome shows that SpiX biotechnology requires adjustments depending on the species in which it has to function. These results are the opportunity to uncover the targeting of meiotic recombination in a widely cultivated crop species and to understand the mechanisms determining sites for double-strand breaks prior to meiotic recombination in wheat
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32

Bagwasi, Gaesejwe. "Response of wheat (Triticum aestivum L.) and barley (Hordeun vulgare L.) to salinity stress". Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/97822.

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Thesis (MSc)--Stellenbosch University, 2015.
ENGLISH ABSTRACT: Good quality water for agricultural use is rapidly becoming a luxury due to competition for this water among the municipal, industrial and agricultural sectors. This has often forced growers to use poor quality water for irrigation. Salinity is one of the main sources of poor water quality and high electrical conductivities (EC’s) due to salinity may become a problem. The aim of this study was to compare the response of South African spring wheat and South African spring barley at germination, seedling growth, vegetative growth, reproductive growth and maturity stage to salinity stress caused by irrigation with saline water. This study was conducted in the laboratory and under controlled glasshouse conditions at the University of Stellenbosch in the Western Cape Province of South Africa. Treatments in trial 1 (incubation trial) were made up of three wheat cultivars (SST 027, SST 056 and SST 087) and three barley cultivars (Nemesia, Erica and Hessekwa) exposed to five EC levels of NaCl solutions (4, 8, 12, 16 and 20 dS m-1) and a control (0 dS m-1) of distilled water, during the germination phase. In trial 2 (pot trial), wheat cultivar SST 027 and barley cultivar SVG 13 were also subjected to the above solutions, but plants were grown till the tillering stage. In trial 3 (pot trial) cultivars used in trial 2 were subjected to five nutrient solutions with EC levels of 1.6, 3, 6, 9 and 12 dS m-1 and allowed to grow till maturity (harvesting stage). Fully balanced nutrient solution with EC = 1.6 dS m-1 was used as a control and NaCl was added to the solutions to obtain the needed EC. In trial 1, final germination percentage (FGP), salt tolerance (ST) and germination rate (GR) were measured at 7 days after incubation. The study showed that when the EC level was increased, FGP, ST and GR of all wheat and barley cultivars tested were decreased. However, significant reduction was only observed at high EC levels with regard to FGP and ST. Wheat cultivars recorded faster GR compared to barley cultivars and tended to be less sensitive to salinity in the germination stage. Cultivars from the same species did not show significant differences. In trial 2, shoot length (SL), root length (RL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW) and root dry weight (RDW) were measured at 35 days after planting (DAP). In general, the study showed that salinity had a significant (P0.05) effect on seedling growth of all measured parameters of both wheat and barley. Mean values for most growth parameters were higher for barley cultivar SVG 13 as compared to wheat cultivar SST 027. However, little evidence was found to show that barley is more salt tolerant than wheat at the seedling stage. In trial 3, selected growth parameters were measured at tillering (28 DAP), booting (54 DAP), flowering (71 DAP) and maturity stage (150 DAP). The study showed that salinity had a significant (P0.05) effect on the vegetative growth, reproductive growth and grain yield of both wheat and barley. Although barley generally produced higher dry weights especially at the early growth stages no clear evidence was found that South African spring barley is more salt tolerant than South African spring wheat.
AFRIKAANSE OPSOMMING: Besproeiingswater met ‘n goeie kwaliteit vir landboukundige gebruik word vinning baie skaars weens kompetisie, a.g.v menslike en industriële gebruik. Produsente word dus dikwels gedwing om water met ‘n swak kwaliteit te gebruik vir besproeiing. ‘n Hoë sout inhoud (brakwater) soos gemeet deur ‘n hoë elektriese geleidingsvermoë (EC), mag dus ‘n problem wees. Die doel van hierdie studie was om te bepaal hoe Suid Afrikaanse lente koring en gars gedurende ontkieming asook saailing-, vegetatiewe-, reproduktiewe- en rypwordingstadiums reageer teenoor soutstremming wat veroorsaak is deur besproeiing met brakwater. Die studie is uitgevoer in laboratoriums en onder gekontrolleerde glashuistoestande by die Universiteit van Stellenbosch in die Weskaap Provinsie van Suid Afrika. Behandelings in die eerste proef (inkubasie studie) het bestaan uit drie koring kultivars (SST 027, SST 056 en SST 087) en drie gars kultivars (Nemesia, Erica en Hessekwa) wat tydens ontkieming benat is met vyf NaCl-oplossings met EC waardes van 4, 8, 12, 16 en 20 dS m-1 onderskeidelik, asook ‘n kontrole met gedistilleerde water (0 dS m-1). In die tweede proef is die koring kultivar, SST 027 en die gars kultivar SVG 13 in ‘n potproef ook aan bogenoemde oplossings blootgestel maar toegelaat om tot die stoelstadium te ontwikkel. In die derde proef is genoemde twee kultivars besproei met vyf voedingsoplossings met EC-waardes van 1.6, 3, 6, 9 en 12 dS m-1 en toegelaat om tot oesstadium te ontwikkel. ‘n Volledig gebalanseerde voedingsoplossing met EC = 1.6 dS m-1 is as kontrole gebruik en NaCl is by ander oplossings gevoeg om die verlangde EC te verkry. In die eerste proef waar die finale ontkiemingspersentasie (FOP), sout toleransie (ST) en ontkiemingstempo (OT) na 7 dae gemeet is, is gevind dat FOP, ST en OT van al die koring en gars kultivars wat getoets is, met toenemende EC gedaal het. Statisties betekenisvolle afnames in FOP en ST is egter slegs by hoë EC waardes waargeneem. Koring kultivars het vinniger ontkiem as gars kultivars en was geneig om meer tolerant teenooor sout stremming te wees vergeleke met gars. Verskille tussen kultivars van dieselfde spesie was egter weglaatbaar klein. In die tweede proef waar plante toegelaat is om te groei tot die stoelstadium (35 dae na plant) is al die gemete planteienskappe (stingel- en wortellengte, asook vars en droë massas van stingels en wortels) van beide gars kultivar, SVG 13 en koring kultivar, SST 027, betekenisvol verlaag deur ‘n toename in EC van die besproeiingswater. Hoewel gars ten opsigte van die meeste gemete eienskappe groter gemiddeldes as koring getoon het, is weinig bewys gevind wat daarop dui dat die getoetsde gars kultivar SVG 13 meer souttolerant is as die koring kultivar SST 027. In die derde proef waar dieselfde koring en gars kultivars vanaf plant tot oestyd besproei is met genoemde voedingsoplossings en metings tydens stoelstadium (28 dae na plant), stamverlenging (54 dae na plant), blomstadium (71 dae na plant) en oesrypstadium (150 dae na plant) gedoen is, is alle gemete vegetatiewe-, reproduktiewe- en opbrengskomponente van beide spesies verlaag deur die soutstremming. Hoewel gars ook in hierdie proef veral gedurende vroeë groeistadiums groter droë massas as koring geproduseer het, is geen konkrete bewyse gevind wat daarop dui dat die getoetsde Suid Afrikaanse lente gars kultivar SVG 13 meer sout tolerant is as die koring kultivar SST 027.
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Murcia, Julian Alejandro Giraldo. "Ação de reguladores vegetais em trigo (Triticum aestivum L.) e cevada (Hordeum vulgare L.)". Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/11/11144/tde-28092016-114839/.

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Os vegetais possuem metabólitos endógenos circulantes nos tecidos, que não necessariamente possuem função nutricional, mas atuam na regulação do crescimento e do desenvolvimento, os hormônios vegetais. Estes, quando utilizados exogenamente, permitem ações planejadas no crescimento das plantas. Igualmente, análogos sintéticos com ação semelhante vêm sendo utilizados na agricultura com o mesmo objetivo. No entanto, o efeito destes reguladores vegetais é variável entre as espécies e em suas fases fenológicas, com necessidade de estudos especificos que propiciem respostas adequadas aos objetivos de sua aplicação. Da mesma forma, encontramos também moléculas capazes de atuar na produção endógena de hormônios, limitando ou induzindo sua produção, afetando os processos de crescimento e desenvolvimento vegetal. A aplicação de tais substâncias na agricultura pode ser benéfica no controle do crescimento vegetal, revertendo a energia produzida pela fotossíntese para a produção dos cultivos. Diversos cultivos, incluindo os de cereais, tendem à um crescimento vegetativo exagerado com o incremento do uso de fertilizantes, causando quedas de produtividade e problemas na colheita devido ao acamamento das plantas. Diante disso, o presente estudo tem como objetivo avaliar as alterações morfológicas e fisiológicas do uso de reguladores vegetais na cultura do trigo (Triticum aestivum L.) e da cevada (Hordeum vulgare L.), visando redução do porte das plantas sem alterações ou com incremento de produção. Os experimentos foram desenvolvidos com ambas espécies de cereais, semeadas em vasos. As aplicações dos tratamentos foram realizadas no início da fase de alongamento do colmo. Nos experimentos, foram avaliados os efeitos da pulverização foliar de diferentes reguladores vegetais que restringem a síntese de giberelina na planta: ácido abscísico (ABA), etil trinexpac (Moddus), daminozide (SADH), ethephon (Ethrel) e cloreto de clormequat (CCC). Foram avaliadas variáveis relacionadas ao retardamento do crescimento vegetativo e à produção de carboidratos, assim como a produção das plantas tratadas, tais como: altura das plantas, índice SPAD, transpiração, condutância estomática e massa seca do caule e espigas. Os dados obtidos foram submetidos à análise de variância e quando significativos, os tratamentos foram comparados pelo teste de Tukey ao nível de 5% de probabilidade. A pulverização de ABA, SADH e Ethrel diminuiu significativamente a massa seca do colmo e promoveu retardamento do crescimento, no entanto, a aplicação de ABA aumentou significativamente o índice de colheita, assim como a massa seca dos grãos. Tratamentos com duas pulverizações de ABA 24 g L-1, com uma semana de intervalo restringiu o alongamento da planta, melhorando a arquitetura da mesma, o teor de clorofila e as trocas gasosas, nas culturas de trigo e cevada, estimulando incremento de 23% na massa seca dos grãos de trigo e de 33% em cevada; assim sendo, a aplicação deste retardante de crescimento pode ser considerada para ser utilizada em lavouras de cereais.
Plant tissues contain circulating endogenous metabolites which not necessarily play nutritional function but can regulate growth, plant hormones, that can be also exogenously applied for planned effects in plant growth. These molecules when sprayed on plants allow desirable effects on the plant growth. Moreover, synthetic analogues with similar action have been used in agriculture for the same purpose. However, the effect of growth regulators is variable among species and their phoenological stages, requiring specific studies that provide appropriate responses to their application. Similarly, there are also capable of acting on endogenous hormone production, limiting or stimulating their biosynthesis and consequently affecting the processes of growth and plant development. The application of such molecules in agriculture can be beneficial in controlling the plant growth, reversing the energy produced by photosynthesis for that growth to agronomic yield of crops. Several crops, including cereals, tend to respond with excessive vegetative growth to the increase of fertilizer application causing productivity decreases and damages to the crop due to the plant lodging. Thus, the present study was carried out to evaluate the morphological and physiological changes by the use of plant growth regulators on wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.), aiming the reduction of the plant size and increases of productivity. The experiments were conducted with both cereal species sown in pots. The applications of the treatments were performed at the beginning of stem elongation stage The effects of foliar application of different plant growth regulators that restrict the gibberellin synthesis in the plant were evaluated: Abscisic acid (ABA), trinexapac-ethyl (Moddus), Daminozide (SADH), Ethephon (Ethrel), and chlormequat chloride (CCC). The following variables were measured: plant height, SPAD index (indirect chlorophyll content), transpiration, stomatal conductance, and dry mass of stems and spikelets. The data were analyzed by the Tukey test at 5% probability level. The spray with ABA, SADH, and Ethrel decreased significantly the dry mass of the stem and promoted growth retardation (plant height). However, the application of ABA increased significantly the harvest index, as well as the dry mass of the grains. Treatments with two sprays of ABA (24 g L-1), with an interval of a week restricted the plant growth in height improving the architecture thereof, chlorophyll content, and gas exchange, resulting in an increment of 23% and 33% in dry matter of grains of wheat and barley, respectively. Therefore, the application of ABA may be considered as an alternative for improving the productivity of cereal crops.
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34

Penckowski, Luis Henrique. "Efeitos do trinexapac-ethyl e do nitrogênio na produtividade da cultura do trigo". UNIVERSIDADE ESTADUAL DE PONTA GROSSA, 2006. http://tede2.uepg.br/jspui/handle/prefix/2185.

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The use of nitrogen in the culture of the wheat seeks the increase of the productivity.However, it also increases the probability of occurrence of the lodgnign. That can be avoided with the application of growth regulators. In that sense, he took place a field experiment, in the city of Castro, PR, seeking to evaluate the effects of the application of the trinexapac-ethyil in different times and of doses of nitrogen on wheat, cultivars (VANTE and BRS 77). The experimental field was blocks at random in factorial outline 4x4 with four repetitions. The treatments consisted of the combination of 100 g i.a ha -1 of applied trinexapac-ethyl among the 1º and 2º visible knot of the wheat, 2º and 3º visible knot, sequential application on half of the dose between the 1º and the 2º visible knot and half between the 2º and 3º visibleknot, besides the witness without trinexapac-ethyl apllication. The doses of nitrogen were of 90, 135, 180 and 225 kg.ha - 1 fot to AVANTE and 60,90, 120 and 150 kg.ha - 1 for to BRS 177. They were certain the stand, tillers numbers for plant, height of plants, diameter of the stem, lengthof the stem, length of thes stem among the leaf flag unitl the insert of the erar, lodging in the antesis and in the pre-cropo haverst, tenor of nitrogen in the leaves, yield and yield componentes and industrial quality of thewheat. The trinexapac-ethyl was efficient in reducing the length of the he/she enters us, reducidng the height of the plants and lodging percentage, being the moment of application of hte trinexapac-ethyl that promotes larger effects in the height of plants in between the 2º and 3º visible knot. The application of hte trinexapac - ethyl mainly in the phase of 1º and 2º visivle knot or 2º and 3º visible knot promoted significant increase of the ears number and in the productivity of grains when compared to the treatment without application of the growth regulator in cultivar AVANTE, what didn t happen for to cultivar BRS 177. The increase of the dose of nitrogen increases the tenor of the element in the plant and it promotes larger lodging factor that can be mininmized by the application of the growth reducer. It didn t happen earnings about the production components and productivity for the increase of the doses of nitrogen, showing that the recommended doses assistg the demands of the cultivars. The industrial quality of hte wheat was not influenced by the trinexapac-ethyl application, except for to cultivate BRS 177 that presented increase of theweight hectolitric in the treatments with trinexapac-ethyl. The increase of the dose of nitrogen increased the amount of gluten humid, dry and of the Force of Gluten (W) and it reduced the weight hectolitric and Falling Number to cultivar BRS 177, in cultivar AVANTE the doses of N didn t influence the parameters regarding the industrial quality
O uso de nitrogênio na cultura do trigo visa o aumento da produtividade. No entanto, aumenta também a probabilidade de ocorrência do acamamento. Que pode ser evitado com a aplicação de reguladores de crescimento. Nesse sentido, realizou-se um experimento de campo, no município de Castro, PR, visando avaliar os efeitos de doses de nitrogênio nas cultivares de trigo AVANTE e BRS 177. O delineamento experimental foi blocos ao acaso em esquema fatorial 4X4 , com quatro repetições para cada cultivar. Os tratamentos constaram da combinação de 100 g i.a ha-1 de trinexapac-ethyl aplicado entre o 1º e o 2º nó visível e metade entre o 2º e o 3º nó visível, além da testemunha sem aplicação. As doses de nitrogênio em cobertura foram de 90, 135, 180 e 225 kg.ha-1 para a cultivar AVANTE e 60,90, 120 e 150 kg.ha-1 para a cultivar BRS 177. Foram avaliados o stand, números de perfilhos por planta, estatura de plantas, diâmetro de colmo, comprimento dos entre-nós, comprimento do caule do último nó e a inserção da espiga, acamamento no florescimento e na pré-colheita, teor de nitrogênio nas folhas, componentes da produção, produtividade e qualidade industrial do trigo. O Trinexapac-ethyl foi eficiente em reduzir o comprimento dos entre-nós, diminuindo a estatura das plantas e a porcentagem de acamamento, sendo o momento da aplicação do trinexapac-ethyl que promove maiores efeitos na estatura de plantas é entre o 2º e 3º nó visível. A aplicação do trinexapac-ethyl principalmente na fase de 1º e 2º nó visível ou 2º e 3º nó visível promoveu aumento significativo do número de espiguetas e na produtividade de grãos quando comparado ao tratamento sem aplicação do regulador de crescimento na cultivar AVANTE, o que não ocorreu para a cultivar BRS 177. O aumento da dose de nitrogênio aumenta o teor do elemento da planta e promove maior acamamento, fator que pode ser minimizado pela aplicação do redutor de crescimento. Não ocorreu ganho sobre os componentes de produção e produtividade pelo aumento das doses de nitrogênio, mostrando que as doses recomendadas atendem as exigências das cultivares. A qualidade industrial do trigo não foi influenciada pela aplicação de trinexapac-ethyl, com exceção da cultivar BRS 177 que apresentou aumento do peso hectolitrico nos tratamentos com trinexapac-ethyl. O aumento da dose de nitrogênio aumentou a quantidade de glúten úmido, seco e da Força de Glúten (W) e diminuiu o peso hectolitrico (PH) e Falling Number (FN) para cultivar BRS 117, na cultivar AVANTE as doses de N não influenciaram os parâmetros referentes à qualidade industrial.
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35

Becerra, Donoso Marcelo Segundo. "Efecto del rastrojo de trigo (Tritricum aestivum L.) sobre la germinación y crecimiento inicial de genotipos de lupino y capacidad alelopática de rastrojo de trigo (Tritricum turgidum L. spp. durum) en descomposición". Tesis, Universidad de Chile, 2010. http://www.repositorio.uchile.cl/handle/2250/112246.

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Memoria para optar al Título Profesional de Ingeniero Agrónomo
En Chile se ha observado problemas de establecimiento y rendimiento de lupino cuando se cultiva después de trigo en condiciones de cero labranza con rastrojos sobre el suelo. Esta inhibición ha sido descrita como efecto alelopático del rastrojo de trigo sobre lupino. Dado que en el país se cultivan dos especies de lupino (L. albus y L. angustifolius) y que el efecto del rastrojo sobre lupino puede ser modificado por las condiciones ambientales, es que los objetivos de este trabajo fueron determinar la sensibilidad intra e interespecífica de dos especies de lupino al extracto de rastrojo de trigo y determinar la capacidad alelopática del residuo de trigo sometido a diferente grado de descomposición. Se realizaron dos ensayos. El primer ensayo fue para determinar la sensibilidad intra e interespecífico de las dos especies de lupino al extracto de rastrojo de trigo y el segundo ensayo fue para evaluar la capacidad alelopática del residuo de trigo sometido a diferente grado de descomposición. Para el primer ensayo se embebió semillas de siete genotipos de Lupinus albus y nueve genotipos de Lupinus angustifolius con extracto de 0, 23, 47 y 70 g de rastrojo de trigo L-1. Se evaluó la capacidad germinativa (CG), valor máximo de germinación (VM), longitud de radícula e hipocotilo (LR y LH), peso fresco de radícula e hipocotilo (PR y PH), relación LR/LH y longitud de plántula (LP). Para el segundo ensayo se dejó 0, 5 y 10 Mg ha-1 de rastrojo sobre la superficie de un suelo que había tenido trigo la temporada anterior. Estas cantidades de rastrojo fueron sometidas a distintos grados de descomposición aplicando tres niveles hídricos mediante una línea de aspersión. Se evaluó la descomposición del rastrojo y su capacidad alelopática. Se encontraron diferencias entre especies. Lupinus angustifolius mostró una mayor sensibilidad al extracto de rastrojo, observándose reducción en el valor máximo de germinación. Esta variable permitió encontrar diferencias inter e intraespecíficas. Las diferencias intraespecíficas en L. albus fueron consecuencia de las diferencias entre genotipos, mientras que las diferencias entre los genotipos de L. angustifolius se debieron a la interacción genotipo * concentración de extracto, por lo que la selección de genotipos tolerantes al extracto de rastrojos debe ser distinta según la especie de lupino. La aplicación de riego, independiente de la cantidad, aumentó la descomposición del rastrojo, mientras que el establecimiento de lupino aumentó con la mayor cantidad de agua aplicada en el periodo de mayor temperatura. El efecto de la cantidad de rastrojo superficial en el establecimiento de lupino fue secundario. Se evidenció un importante efecto alelopático en el suelo que sólo tenía raíces de trigo de la temporada anterior. El bioensayo con extractos acuosos de rastrojo de trigo afectó la germinación de lupino, pero no identificó diferencias alelopáticas entre los residuos con distinto grado de descomposición.
Problems of lupine establishment and yield have been observed in Chile when this crop is grown following wheat under zero tillage conditions with stubble on the ground. This inhibition has been described as an allelopathic effect of wheat stubble on lupine. Since two species of lupine (L. albus and L. angustifolius) are grown in the country and the effect of stubble on lupine may be modified by environmental conditions, the objectives of this study were to determine the intra- and inter-sensitivity of these lupine species to wheat stubble extract and to determine the allelopathic capacity of wheat residue subjected to varying degrees of decomposition. Two tests were carried out for this purpose. The first one was to determine the intra-and interspecific sensitivity of the two lupine species to wheat stubble extract and the second one was to evaluate the allelopathic capacity of wheat residue subjected to varying degrees of decomposition. For the first trial seeds of seven genotypes of Lupinus albus and nine genotypes of Lupinus angustifolius were imbibed in 0, 23, 47 and 70g of wheat stubble extract L-1. Germinative capacity (CG), maximum germination (VM), radicle and hypocotyl length (RL and HL), fresh weight of radicle and hypocotyl (PR and PH), RL/HL ratio and seedling length (LP) were evaluated. For the second test, 0, 5 and 10 mg ha-1 of stubble were left on the soil surface which had had wheat the previous season. These amounts of stubble were subjected to different degrees of decomposition using three water levels through a spraying line. Stubble decomposition and its allelopathic capacity were evaluated. Differences were found between species. Lupinus angustifolius showed a greater sensitivity to stubble extract, with a decrease in the maximum germination value being observed. This variable allowed to find inter- and intraspecific differences. The instraspecific differences in L. albus resulted from the differences among genotypes, while differences among L. angustifolius genotypes were due to the genotype-extract interaction, for which reason the selection of genotypes tolerant to stubble extract must be different depending on the lupine species. Irrigation application, irrespective of its amount, increased stubble decomposition, whereas lupine establishment increased with the greatest amount of water applied at the time of highest temperature. The effect of surface stubble amount on lupine establishment was secondary. An important allelopathic effect was seen on the soil which only exhibited roots from the previous season’s wheat. The bioassay with aqueous extracts of wheat stubble affected lupine germination, but did not identify allelopathic differences among residues with different levels of decomposition.
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36

Plassé, Caroline. "Molecular and phenotypic diversity of wheat (Triticum aestivum L.) for winter hardiness /". Zürich : ETH, 2007. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17544.

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37

Folck, Anne-Christine. "Posttranskriptionale Geninaktivierung der [alpha]-Gliadine [Alpha-Gliadine] in Weizen (Triticum aestivum L.)". [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972504575.

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38

Jukanti, Aravind Kumar. "Molecular and Biochemical Characterization of Wheat (Triticum aestivum. L) Polyphenol Oxidases (PPOs)". Thesis, Montana State University, 2005. http://etd.lib.montana.edu/etd/2005/jukanti/JukantiA1205.pdf.

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Polyphenol oxidases (PPOs) from several plant species, including wheat, have been implicated in the undesirable brown discoloration of food products. Wheat (Triticum aestivum L.) represents an interesting system to advance our understanding of plant PPO function for two important reasons, namely (1) the large size an complexity of its (allohexaploid) genome, and (2) its economic importance. Prior to this study, the molecular and biochemical properties of wheat PPOs were largely unknown. To remedy this situation, we have performed several BLAST searches of expressed sequence tag (EST) databases, using a known wheat PPO sequence as a search tool. This study suggested the presence of at least six PPO genes in hexaploid wheat, falling into two different phylogenetic clusters of three genes each. Presence of a wheat PPO multigene family was confirmed by Southern blotting. A combination of biochemical (enzyme purification and mass spectrometric analysis) and molecular (Northern) approaches confirmed that members of one cluster are not expressed in the developing kernels and senescing flag leaves, while regulation of one or several members of the other gene cluster controls PPO activity in these tissues. Our data, including immunoblotting and enzyme activity studies, further indicated that wheat PPOs are synthesized as inactive precursor (early kernel development) which are proteolytically processed and activated as the kernels mature. Activation of PPO precursor proteins was also demonstrated in vitro, in presence of purified trypsin. In these experiments, PPO activity increased during the first four hours and remained stable thereafter, indicating that the protein domains responsible for catalytic activity are quite stable. Research performed as a part of this dissertation has also demonstrated that wheat PPO activity is influenced by strong anionic detergents such as SDS and N-lauroylsarcosine. The corresponding experiments indicated that these detergents influenced both enzyme extraction and activity, at least in high-PPO wheat varieties. This work also has practical aspects, making PPO assays (as used by breeders for germplasm screening) more robust. In conclusion, as a result of this dissertation, wheat PPOs have emerged as a fascinating example of a plant multigene family with complex transcriptional and posttranscriptional regulation.
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39

Taeb, Mohammad. "The genetics of salt and waterlogging tolerance in wheat (Triticum aestivum L.)". Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239092.

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40

Aperavičiūtė, Vaida. "Skirtingų veislių žieminių kviečių (Triticum aestivum L.) laikomų grūdų kokybės rodiklių palyginimas". Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140613_102223-82018.

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Tyrimo objektas žieminių kviečių veislių 'Julius', 'Leifer', 'Operetka' ir 'Skagen' technologinių savybių kitimas laikymo metu. Tyrimo tikslas ištirti skirtingų veislių žieminių kviečių technologinių savybių kitimo dėsningumus priklausomai nuo laikymo trukmės. Uždaviniai 1. Ištirti skirtingų veislių žieminių kviečių grūdų kokybę tik nuėmus derlių. 2. Įvertinti laikomų grūdų kokybę. 3. Apibendrinti grūdų technologinių savybių kitimo dėsningumus. Tyrimo metodai 2012–2013 m. Pakruojo rajone buvo auginti kviečiai. Žieminių kviečių derlius nuimtas 2013 m. rugpjūčio 20 d. Atlikti kviečių kokybės rodiklių tyrimai. Standartiniais metodais ištirta: grūdų drėgnis, baltymų, šlapiojo glitimo, krakmolo kiekis, sedimentacijos rodiklis, kritimo skaičius, piltinis tankis, pesticidų likučiai. Vėliau kviečių mėginiai laikomi Diliauskų elevatoriuje, sandėlyje dėžutėse po 4 kg, trimis pakartojimais. Kas mėnesį atliekami kviečių rodiklių kokybės tyrimai. Tyrimo rezultatai. Žieminių kviečių auginimo agrotechnikoje naudojant pesticidus pagal rekomendacijas ir prisilaikant leidžiamų normų, pesticidų likučiai grūduose nesikaupia. Laikant sausus grūdus sandėlyje jų drėgmė padidėja, baltymų kiekis ir saiko svoris nesikeičia. Glitimo kiekis laikomuose žieminiuose kviečiuose padidėjo nežymiai. Krakmolo kiekis laikomuose žieminiuose kviečiuose didėjo vidutiniškai 1,3 %. Laikymo laikotarpiu sedimentacijos vertės žieminiuose kviečiuose mažėjo, vidutiniškai 6,45 ml. Kritimo skaičius turėjo tendensiją... [toliau žr. visą tekstą]
Research object winter wheat varieties 'Julius', 'Leifer', 'Operetka' and 'Skagen' technological characteristics change during storage. Research aim explore the different varieties of winter wheat technological properties change dependent on the storage time. Objectives 1. First investigate the different varieties of winter wheat grain quality after the harvest. 2. Evaluate the quality of stored grain. 3. Summarize the technological properties of grain variation patterns. Research methods 2012-2013 year Pakruojo area were grown winter wheat. Winter wheat harvested in 2013 August 20 d. Perform quality in wheat research. Analyzed by standard methods of grain moisture content, protein, wet gluten, starch content, sedimentation value, falling number, bulk density, pesticide residues. Winter wheat samples kept in Diliauskų elevators, stock boxes of 4 kg, with three replications. Monthly indicators made of wheat quality tests. Research results. Winter wheat agricultural equipment, the use of pesticides in accordance with guidelines and in compliance with the allowable limits of pesticide residues in grain accumulation. Keeping dry grain stock of humidity increases the protein content moderation and weight does not change. Gluten content stored in winter wheat increased slightly . The starch content is stored in winter wheat increased an average of 1, 3 % . The storage period of sedimentation values in winter wheat decreased an average of 6, 45 ml . Falling number was... [to full text]
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41

Bassoi, Manoel Carlos. "Quantitative trait analysis of grain dormancy in wheat (Triticum aestivum L. Thell)". Thesis, University of East Anglia, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251389.

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42

Nabipour, Majid. "Comparative ecophysiology of two cultivars of wheat (Triticum aestivum L.) under drought". Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.327275.

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43

Jiang, Wen Zhi. "Comparative study of manganese efficiency in UK wheat (Triticum aestivum L.) cultivars". Thesis, University of Essex, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.272582.

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44

Macbeth, Jacqueline Elizabeth. "The prediction of mature grain weight in winter wheat : (Triticum aestivum, L.)". Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309808.

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45

Koksel, Havva Filiz. "Bubble size distributions in non-yeasted wheat (Triticum aestivum L.) flour dough". Journal of Cereal Science, 2012. http://hdl.handle.net/1993/30114.

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Bread owes its appeal to its aerated structure which directly relies on the bubbles entrained into the dough during mixing. If the bubble size distribution (BSD) in the dough can be determined at the end of mixing, then the resulting loaf quality could be predicted before bread is fully manufactured. However, non-invasively monitoring the structure of a fragile opaque soft solid such as dough is challenging. This thesis addressed the challenge by determining dough’s BSD and its evolution using ultrasound and X-ray microtomography. Using a resonant scattering model and the frequency dependence of the ultrasonic parameters measured in the dough, the change in the BSD in dough (made without yeast) with time as a result of disproportionation was determined. At 30 min after mixing, the median radius (R0) of the lognormal BSD was 6.5 microns. Converting the BSD to the radius dependence of bubble volume fraction (BVF(R)), R0V (the median radius of BVF(R)) was 66.4 microns and increased 18 % in the succeeding 90 min. In order to validate the bubble sizes determined ultrasonically, X-rays from a synchrotron source were utilized to examine dough’s microstructure. Large numbers of very small bubbles were discovered and it was apparent that lognormality did not describe the BSDs. Nevertheless, lognormal characterization of the BVF(R) was appropriate. At 30 min after mixing R0V of the BVF(R) was 32.5 microns and it increased by 20 % in the succeeding 90 min, supporting the ultrasonic quantification of bubble volume changes due to disproportionation. Changes in the mode, median and mean of the BVF(R) with time after mixing had the same trend for ultrasound and for X-ray microtomography. The time evolution of the mode of the BVF(R) obtained by ultrasound and X-ray microtomography matched very well; both increasing linearly as a function of time. Ultrasonic assessments of bubble sizes and their changes with time are very encouraging, but the ultrasonic model should use distribution functions that precisely define the empirical data, perhaps not making ‘pre-assumptions’ of lognormality for the BSD data.
February 2015
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46

Bierman, Anandi. "Mapping and survey sequencing of Dn resistance genes in Triticum aestivum L". Thesis, Stellenbosch : Stellenbosch University, 2015. http://hdl.handle.net/10019.1/96912.

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Thesis (PhD)--Stellenbosch University, 2015
ENGLISH ABSTRACT : Diuraphis noxia Kurdjumov (Russian Wheat Aphid; RWA) is a pest of wheat and barley that has spread from its home range in the fertile crescent to most wheat producing countries except Australia. Since its first introduction to South Africa and the USA in the late 20th century, breeding programs for wheat phenotypes resistant to the aphid were put in place. Conventional breeding practices rely on phenotypic screening to verify traits carried by offspring and genetic tools such as marker assisted selection (MAS) have greatly aided this process in speed and accuracy. The size and complexity of the wheat genome, its allopolyploid nature and repetitive elements have, however, posed a challenge to studies on the genetics of this cereal crop. Many studies have focused on chromosome 3B which is the largest of the wheat chromosomes and easily separated from the redundant genomic background by techniques such as flow cytometry. The similarity in size of the remaining chromosomes however, limits the application of flow cytometry to their isolation. Databases such as Grain-Genes (http://wheat.pw.usda.gov/GG2/index.shtml) house marker data from various mapping studies for all wheat chromosomes and in 2014 the International Wheat Genome Sequencing Consortium (IWGSC) completed the draft genome sequence of wheat categorized by chromosome. Sources of resistance (Dn resistance genes) against RWA are located on chromosome 7D. but despite the marker and sequence data available currently, mapping studies specific for the Dn resistance genes are few. Additionally, sequence data available is derived from cultivars susceptible to RWA and is not comprehensively annotated and assembled in many cases. In this study, we demonstrate a novel, combined approach to isolate and characterize the Dn resistance genes through the use of a genetic map constructed from Amplified Fragment Length Polymorphism (AFLP), Expressed Sequence Tag (EST) and microsatellite markers and a physical map constructed from Next Generation Sequencing (NGS) data of ditelosomic chromosomes (7DS and 7DL) isolated by microdissection on the PALM microbeam system. A 122.8 cM genetic map was produced from 38 polymorphic AFLP markers and two ESTs with the microsatellite Xgwm111 as anchor to related genetic maps. Through comparison to maps available on GrainGenes the location of the Dn1 resistance gene was narrowed down to a deletion bin (7DS5-0.36-0.62) on the short arm of chromosome 7D with an AFLP marker (E-ACT/M-CTG_0270.84) mapping closely at 3.5 cM and two ESTs mapping at 15.3 cM and 15.9 cM from Dn1. Isolation of individual chromosome arms 7DS and 7DL using the PALM Microbeam system allowed sequencing of the chromosome without the redundancy of the remainder of the hexaploid genome. Through isolating the chromosome arms in this way, a >80-fold reduction in genome size was achieved as well as a major reduction in repetitive elements. Analysis of the sequencing data confirmed that 7DL is the physically shorter arm of the chromosome though it contains the majority of protein coding sequences.
AFRIKAANSE OPSOMMING : Diuraphis noxia Kurdjumov (Russiese koring-luis; RWA) is « pes wat op koring en gars voorkom. Die pes het vanaf sy tuiste in die midde Ooste na meeste koringproduserende lande behalwe Australië versprei. Sedert die eerste bekendstelling van RWA in Suid Afrika en die VSA in die vroeë 20ste eeu is teelprogramme ten gunste van koring lyne met weerstand teen RWA begin. Tradisionele teelprogramme maak op fisieise observasie van die fenotipe staat om te verifieer of plante in die nageslag die gewenste eienskap dra. Genetiese metodes soos merkerondersteunde seleksie (MAS) versnel hierdie selekteringsproses grootliks. Die grootte en kompleksiteit van die koring genoom asook die polyploïde en herhalende natuur daarvan is « groot hindernis vir genetiese studies van hierdie graangewas. Baie studies het op chromosoom 3B gefokus wat die grootste van die koring chromosome is en dus maklik vanaf die res van die oorbodige genomiese agtergond deur tegnieke soos vloeisitometrie geskei word. Die ooreenkoms in grootte tussen die res van die chromosome bemoeilik die toepassing van vloeisitometrie om hulle te isoleer. Databasisse soos GrainGenes (http://wheat.pw.usda.gov/GG2/index.shtml) bevat merker data vanaf verskeie karterings-studies vir al die chromosome en in 2014 het die "International Wheat Genome Sequencing Consortium"(IWGSC) die voorlopige basispaarvolgorde van die koring genoom bekendgestel, gekategoriseer volgens chromosoom. Weerstandsbronne (Dn weerstandsgene) teen RWA kom meestal op chromosoom 7D voor. Ten spyte van merker en basispaarvolgorde data tans beskikbaar is karterings-studies spesifiek tot die Dn gene skaars en basispaarvolgorde data is vanaf kultivars afkomstig wat nie weerstandbiedend teen RWA is nie en waarvan die annotasie en samestelling baie keer nie goed is nie. In hierdie studie demonstreer ons « nuwe, gekombineerde aanslag om die Dn weerstandsgene te isoleer en karakteriseer deur van « genetiese kaart opgestel met "Amplified Fragment Length Polymorphism"(AFLP), "Expressed Sequence Tag"(EST) en mikrosatelliet merkers asook « fisiese kaart saamgestel deur die volgende-generasiebasispaarvolgordebepaling van ditelosomiese chromosome (7DS en 7DL) geïsoleer deur mikrodisseksie met die "PALM Microbeam"sisteem gebruik te maak. « Genetiese kaart van 122.8 cM was met 38 polimorfiese AFLP merkers en twee EST merkers geskep. Die mikrosatelliet, Xgwm111, is ook ingesluit en het as anker vir verwante genetiese-kaarte gedien. Deur vergelyking met genetiese-kaarte op GrainGenes is die posisie van die Dn1 weerstandsgeen vernou na « delesie bin (7DS5-0.36-0.62) op die kort arm van chromosoom 7D met « AFLP merker (EACT/ M-CTG_0270.84) wat ongeveer 3.5 cM vanaf die geen karteer. Die twee EST merkers is 15.3 cM en 15.9 cM vanaf die geen gekarteer. Isolering van die individuele chromosoom arms, 7DS en 7DL, deur van die "PALM Microbeam"sisteem gebruik te maak het basispaarvolgordebepaling van die chromosoom toegelaat sonder die oortolligheid van die res van die hexaploïde genoom. Deur die chromosoom so te isoleer is « >80-maal verkleining in genoom grootte bereik insluitend « groot reduksie in herhalende elemente. Analise van die data vanaf basispaarvolgordebepaling het bevestig dat chromosoom 7D die fisiese kleiner chromosoom is maar dat dit die meerderheid van proteïn koderende basispaarvolgordes bevat.
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47

Fisher, Nadia Mitilda. "Gene silencing in bread wheat (Triticum aestivum L.) following a biolistics approach". Thesis, Stellenbosch : Stellenbosch University, 2014. http://hdl.handle.net/10019.1/86591.

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Thesis (MSc)--Stellenbosch University, 2014.
ENGLISH ABSTRACT: Global food security is hampered by a variety of insects/pest and plant diseases. In wheat, the Russian wheat aphid (RWA) is a significant pest problem in many areas of the world. Wheat has developed defensive mechanisms against the RWA over time which are activated upon feeding. One such mechanism is the hypersensitive response (HR) which is effective against phloem-feeding insects i.e. D. noxia (Diuraphis noxia, Kurdjumov, RWA). In this study, two genes associated with the hypersensitive response i.e. ascorbate peroxidase (APX) and glutathione S transferase (GSTF6b) were investigated to elucidate their function in the defensive mechanism of wheat using a reverse genetic approach i.e. particle bombardment. This study has succeeded in the established of a tissue culture and transformation system which generated three genetically modified wheat plants with decreased resistance to RWA feeding due to gene silencing. The establishment of this system enabled to test the association of defensive related genes in wheat to RWA resistance. Expression analysis performed on obtained transgenics before and after RWA infestation reavealed that the silenced plants were more susceptible to RWA feeding. Chlorosis was observed in the Gamtoos-S-APX transgenic plant which is an indicator of oxidative damage to the photosynthetic machinery of the plant. Decreased GSTF6b transcripts was found in the transgenic Gamtoos-S-GSTF6b and transgenic Gamtoos-R-GSTF6b transgenic plants but no visible symptoms of infestation was observed in these two plants. Resistance breeding could be strengthened by developing broad spectrum resistance plants by incorporating wheat defensive related genes with known function into the breeding programs. The use of this transformation system will allow rapid identification and introduction of agronomically important genes by upregulating these genes to enhance bread wheat against aphid infestation.
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48

Guarienti, Eliana Maria. "Efeito de variaveis meteorologicas na qualidade industrial de trigo (Triticum aestivum L.)". [s.n.], 2001. http://repositorio.unicamp.br/jspui/handle/REPOSIP/256045.

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Orientador: Cesar Francisco Ciacco
Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-07-29T03:34:21Z (GMT). No. of bitstreams: 1 Guarienti_ElianaMaria_D.pdf: 47831149 bytes, checksum: 789ef3a639debad12e503ca04f295a96 (MD5) Previous issue date: 2001
Resumo: Cerca de 90 % da produção de trigo no Brasil está localizada nos estados do Paraná, do Rio Grande do Sul e de Santa Catarina. Nesses estados, a variabilidade climática é muito grande, de tal forma que a produção tritícola é uma atividade de risco. No Brasil, tem sido pouco estudada a influência das variáveis meteorológicas na qualidade industrial de trigo. O trabalho teve por objetivos: verificar quais são as principais variáveis meteorológicas que afetam a qualidade industrial de trigo e em qual período (em dias que antecedem à colheita) essa influência ocorre, na região tritícola sul-brasileira. Neste estudo, foram utilizados dados de experimentos com o trigo Embrapa 16, conduzidos durante os anos de 1990 a 1998. As variáveis meteorológicas analisadas foram: médias das temperaturas mínima, máxima e média do ar e da umidade relativa do ar, somatórios da precipitação pluvial e da radiação solar global e balanço hídrico climático (déficit e excesso hídrico do solo). Em laboratório, foram realizadas as seguintes análises: peso do hectolitro, peso de mil grãos, extração experimental de farinha, alveografia, número de queda, microssedimentação com dodecil sulfato de sódio e rendimento de grãos. Para fins estatísticos, foram criados quatro tipos de arquivos, constituindo quatro subperíodos (de cinco em cinco, de dez em dez, de quinze em quinze e de vinte em vinte dias anteriores à data de colheita), a contar de um até oitenta dias anteriores à colheita. As análises estatísticas realizadas foram: análise de componentes principais, correlações múltiplas, regressões lineares simples e múltiplas e regressões polinomiais. Verificou-se que: a) a precipitação pluvial, a umidade relativa do ar e o excesso de umidade do solo foram as variáveis meteorológicas que mais influenciaram negativamente o peso do hectolitro, o peso de mil grãos, a extração experimental de farinha, a força geral de glúten, a relação P/L, o número de queda e o rendimento de grãos de trigo; b) a influência do conjunto das variáveis meteorológicas é manifestada por uma sucessão de diferentes eventos que ora prejudicam, ora favorecem a expressão da qualidade industrial e do rendimento de grãos de trigo; c) os resultados de regressão não permitiram a previsibilidade das características de qualidade industrial e do rendimento de grãos, em função das variáveis meteorológicas.
Abstract: About 90% of the wheat production in Brazil is located in Paraná, Rio Grande do Sul, and Santa Catarina states. In these states, the climatic variability is very great, so that wheat production is a risk activity. The influence of meteorological variables in the wheat industrial quality has been greatly considered in the Brazil. The work aimed: to verify which are the main meteorological variables that affect the industrial quality of wheat and in which period (in days before crop harvest), this influence occurs in the southem wheat Brazilian region. In this study, trials data obtained in the 1990-1998 period with Embrapa 16 wheat were used. The meteorological variables analyzed were: averages of the minimum, maximum and average temperatures and relative humidity, the sum of rainfall and global solar radiation, and water balance (water deficit and excess). In laboratory, the following analyses were obtained: test weight, thousand kemel weight, experimental milling, alveograph, falling number, dodecyl sulphate microssedimentation test, and grain yield. For statistical purposes, four types of files were generated, constituting four períods (five in five, ten in ten, fifteen in fifteen, and twenty in twenty days prior to crop harvest) trom the eighty days preceding crop harvest. Statistical analyses were: analysis of principais components, multiple correlations, simple and multiple linear regressions, and polinomial regressions. It was verified that: a) rainfall, relative humidity, and water excess were the meteorological variables that more negatively influenced the test weight, thousand kemel weight, milling quality, gluten strength, P/L relation, falling number, and grain yield; b) the influence of the meteorological variables is evidenced by a succession of different events that sometimes harm and at other times favor the industrial quality and grain yield expression; c) the regression results did not allow the previsibility of industrial quality characteristics and grain yield, by meteorological variables.
Doutorado
Doutor em Tecnologia de Alimentos
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49

Mohajeri, Naraghi Sepehr. "Genetics of End-Use Quality Characteristics in Spring Wheat (Triticum Aestivum L.)". Diss., North Dakota State University, 2017. https://hdl.handle.net/10365/26684.

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Wheat (Triticum aestivum L.) is one of the most important crops consumed by humans around the world. Improving the end-use quality traits is one of the major objectives in wheat breeding programs. However, little is known about the genomic regions controlling these traits. Discovering the genetic architecture underlying important end-use quality traits can accelerate breeding via marker-assisted selection (MAS) in addition to providing genomic and biological information. Therefore, for this dissertation, a quantitative trait loci (QTL) mapping and a genome-wide association study (GWAS) were conducted to identify QTL for 16 end-use quality traits, including the grain protein content, flour extraction rate, eight mixograph-related parameters, and six baking-related properties. A population of 127 recombinant inbred lines (RILs) from a cross between Glenn (PI-639273) and Traverse (PI-642780) was developed for the QTL mapping study, and an association panel of 355 elite spring wheat lines was used for the GWAS study. The phenotyping of these traits was performed in nine environments in North Dakota, USA, over a three-year period. The genotyping for both the RIL population and association panel was conducted using the wheat Illumina iSelect 90K SNP assay. A total of 76 additive QTL (A-QTL) and 73 digenic epistatic QTL (DE-QTL) were found for the 16 end-use quality traits in the QTL mapping study. These QTL were distributed across all wheat chromosomes except chromosome 3D. Overall, 12 stable major A-QTL and three stable DE-QTL were identified for the end-use quality traits in the QTL mapping study, indicating that both A-QTL and DE-QTL played an important role in controlling end-use quality traits. In addition to the QTL mapping study, a total of 91 significant marker?trait associations (MTA) were identified for the end-use quality traits in the GWAS study. These MTA were distributed across all wheat chromosomes except chromosome 4D. Overall, the current study identified multiple novel stable QTL that could be used in MAS for end-use quality trait improvement in wheat breeding programs.
North Dakota Wheat Commission
North Dakota State University. Department of Plant Sciences
Hard Red Spring Wheat Program
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50

Rhazi, Larbi. "Formation des polymères gluténiques du grain de blé tendre : Triticum aestivum L". Toulouse, INPT, 2003. http://www.theses.fr/2003INPT010G.

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De nombreuses questions sur la formation des polymères gluténiques dans le grain de blé tendre et leur structure finale demeurent sans réponse. Ainsi la présente étude est effectuée pour mieux comprendre la formation et la structure des agrégats gluténiques. Nous nous sommes attachés à préciser les mécanismes de formation des polymères gluténiques durant le développement du grain dans la mesure où ces associations protéiques conditionnent en grande partie la qualité d'utilisation de la farine de blé tendre. En étudiant l'évolution du statut rédox des protéines de réserve au cours du développement du grain il nous a été notamment possible de démontrer que la formation des très hauts polymères est liée à un phénomène d'oxydation des groupements sulfhydryls libres portés par les sous-unités gluténiques. Ce phénomène, favorisé par le rapprochement des structures polymériques au cours de la séshydratation, conduit à l'apparition de nouvelles liaisons disulfures intermoléculaires et se traduit à la fois par une augmentation de la masse moléculaire et une augmentation du degré de compacité des agrégats gluténiques. Nous avons également pu démontrer que le glutathion était capable de se lier de manière prépondérante à la fraction gluténique limitant ainsi ses capacités d'association lors de la dessiccation. Enfin, une étude exploratoire de modélisation moléculaire a été entreprise afin de proposer une démarche méthodologique permettant d'appréhender les capacités d'interaction des sous-unité gluténiques de haut poids moléculaire.
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