Literatura científica selecionada sobre o tema "Trichoderma reesei – Innovation"
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Artigos de revistas sobre o assunto "Trichoderma reesei – Innovation"
NEAGU, Diana, Jacqueline DESTAIN, Phillipe THONART e Carmen SOCACIU. "Trichoderma Reesei Cellulase Produced by Submerged Versus Solid State Fermentations". Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture 69, n.º 2 (20 de dezembro de 2012). http://dx.doi.org/10.15835/buasvmcn-agr:8780.
Texto completo da fonteTeses / dissertações sobre o assunto "Trichoderma reesei – Innovation"
Alharake, Jawad. "Study of genetic factors involved in enzyme secretion in hyperproductive strains of Trichoderma reesei". Electronic Thesis or Diss., université Paris-Saclay, 2023. http://www.theses.fr/2023UPASB061.
Texto completo da fonteFossil fuels are a major contributor to global warming, and their non-renewable nature is an impediment for building sustainable societies. In this context, second generation biofuels represent an attractive and more environmentally friendly alternative. The process of second-generation biofuel production consists of several steps including a physicochemical pretreatment of lignocellulosic (non-edible) biomass, the enzymatic hydrolysis of cellulose into glucose and the fermentation of simple sugars into biofuels, such as bioethanol. One of the main bottlenecks for a large implementation of this process is, however, the relatively high cost of hydrolytic enzymes, namely cellulases, used to deconstruct the pretreated lignocellulosic biomass into fermentable sugars.The filamentous fungus Trichoderma reesei is the preferred choice for industrial production of cellulases since it has hyperproduction and hypersecretion capacities. Industrial strains of T. reesei can secrete up to 100 g/L of cellulases in controlled industrial fermenters. In particular, the mutant strain Rut- C30 is a reference hyperproducer strain, but our incomplete understanding of its enhanced secretion system complicates further improvement of its hypersecretion capacity by genetic engineering. Therefore, this work aimed at unravelling the regulatory pathways controlling secretion and the secretion stress response in order to identify bottlenecks and to develop new strains with enhanced secretion capacity in the future.To this end, transcriptomic data were generated from cultivations of T. reesei Rut-C30 in different secretion stress conditions which allowed to identify potential components of secretion regulation that were targeted for deletion. As a complementary approach, mining of transcriptomic data obtained with other filamentous fungi in secretion stress conditions revealed further target genes potentially involved in the regulation of the secretion pathway. Finally, nine genes were deleted in the Rut-C30 strain, and the resulting strains phenotypically characterized. All of them displayed reduced growth and showed altered protein secretion behavior. RNA sequencing was performed on ∆res2, ∆rpn4 and ∆snd1 mutant strains and compared to that of Rut-C30 in the same culture conditions. Neither of the three transcription factors impacts transcription of genes involved in secretion or the secretion stress response in our conditions. However, in all three mutants, genes encoding enzymes of lipid metabolism are differentially expressed which could affect secretion in an indirect way. The results represent first clues to alleviate bottlenecks in secretion in T. reesei Rut-C30 and pave the way to develop strains with still improved secretion capacity
Chan, Ho Tong Laetitia. "Amélioration du champignon cellulolytique Trichoderma reesei par reproduction sexuée". Thesis, Paris, Institut agronomique, vétérinaire et forestier de France, 2017. http://www.theses.fr/2017IAVF0017.
Texto completo da fonteGenetic engineering of Trichoderma reesei is one of the solutions considered to reduce the cost of the enzymatic hydrolysis step in second-generation ethanol production processes. It allows the improvement of the secretory performances of this cellulase producer fungus and the properties of its enzymes. Like many industrial fungi, T. reesei has long been considered to possess exclusively an asexual cycle. The recent discovery of a sexual reproduction cycle in this filamentous fungus opens up new prospects of improvement for strains used in biotechnology. However, all industrial strains of T. reesei are derived from the wild isolate QM6a and therefore possess the MAT1-2 mating type and are female sterile.The aim of this work was to develop sexual reproduction as a genetic tool and to improve the performance of the industrial strains. In combination with the genetic engineering tools, it would enable combination of interesting traits or generation of new ones, improve strains stability by purging deleterious mutations, selection markers elimination and identification of genes and mutations responsible of interesting characteristics.The first part of this work was dedicated to the optimization of sexual reproduction and to the study of the steps and mechanisms of sexual reproduction between wild-type and hyperproducer strains. In a second step, we set up sexual reproduction between female sterile hyperproducer strains, using the original "assistant strain" strategy, which resulted in the partial restoration of their sexual reproduction. A study of the fertilization step was also initiated, as we suspect it to be the blocking point in our strategy. In a second part, we took advantage of sexual reproduction between wild-type and hyperproducer strains for (i) the generation of a MAT1-1 mating-type strain compatible with all industrial strains, female fertile and possessing improved β-glucosidase activity and (ii) the implementation of a bulk segregation analysis associated with high-throughput sequencing techniques to identify mutations involved in phenotypes of interest
Filiatrault, chastel Camille. "Exploration de sécrétomes d'Aspergillus spp. en vue de la complémentation du cocktail cellulolytique de Trichoderma reesei". Thesis, Paris, Institut agronomique, vétérinaire et forestier de France, 2019. http://www.theses.fr/2019IAVF0021.
Texto completo da fonteLignocellulosic biomass is considered as a promising alternative to fossil resources. This abundant and renewable feedstock, derived from plant cell wall, is composed of polysaccharides and aromatic polymers linked together to form a highly resistant structure. Its transformation into bioethanol or other products requires the degradation of polysaccharides into fermentable sugars, using enzymes such as cellulases, hemicellulases and lytic polysaccharide monooxygenases (LPMOs). These enzymes are secreted by biomass-degrading microorganisms, such as bacteria and filamentous fungi. The most commonly used is Trichoderma reesei, a fungal species that is known for its efficient secretion of cellulases, but has a low diversity of other enzymes. Enzymatic hydrolysis is still a bottleneck for the cost-effective utilization of lignocellulosic biomass, and needs to be optimized. Providing activities that are missing in T. reesei using enzymes produced by other fungal organisms is a promising approach, which has been implemented during this PhD project.Using a proteomic approach, different Aspergillus strains were studied in order to find enzymatic activities that could improve the hydrolysis of pretreated biomass. For this purpose, 5 strains were grown on 3 inducers, and the protein content of these different secretomes was determined by mass spectrometry. The secretomes were then tested for their ability to improve hydrolysis yields by supplementing a T. reesei cocktail. Analysis of the protein content of some of the best-performing secretomes revealed the presence of a protein belonging to a putative family of LPMO. Several proteins of this family were recombinantly produced and tested on different substrates in the presence of redox partners. Oxidative cleavage of cellulose was demonstrated, leading to the creation of a new LPMO family, called AA16. The pretreatment of cellulosic substrates by AA16 LPMOs resulted in a significant improvement of their hydrolysis by a cellulase from T. reesei, demonstrating their potential interest for biotechnological applications in biorefineries
Trabalhos de conferências sobre o assunto "Trichoderma reesei – Innovation"
Anindyawati, Trisanti, Raditya Putra, Yuliawati, Kartika Sari Dewi, Asrul Muhamad Fuad e Yanni Sudiyani. "Heterologous expression of Trichoderma reesei exoglucanase (Cel6A) in Pichia pastoris under the control of GAP promoter". In PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCES AND MEDICAL ENGINEERING (ICBME2019): Towards innovative research and cross-disciplinary collaborations. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5125548.
Texto completo da fonte