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Artigos de revistas sobre o tema "Stabilité in vitro"

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Autor: Grafiati

Publicado: 27 de julho de 2024

Última modificação: 28 de julho de 2024

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1

Basille, C., L. Hesters, M. C. Bourrier, R. Frydman, R. Fanchin e N. Frydman. "La stabilité des résultats en fécondation in vitro est-elle possible ?" Journal de Gynécologie Obstétrique et Biologie de la Reproduction 38, n.º 4 (junho de 2009): 312–20. http://dx.doi.org/10.1016/j.jgyn.2009.03.005.

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2

Rode, A., C. Hartmann, M. Dron, E. Picard e F. Quetier. "Stabilité de l'ADN chloroplastique et de l'ADN mitochondrial isolés de lignées deTriticum aestivumobtenues par androgenésein vitro". Bulletin de la Société Botanique de France. Actualités Botaniques 133, n.º 4 (janeiro de 1986): 74. http://dx.doi.org/10.1080/01811789.1986.10826804.

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3

Aldrees, Abdullah M., Sahal A. Al-Foraidi, Mohammed S. Murayshed e Khalid A. Almoammar. "Stabilité chromatique et dégradation de la force de chaînettes élastomériques orthodontiques transparentes : une étude in vitro". International Orthodontics 13, n.º 3 (setembro de 2015): 287–301. http://dx.doi.org/10.1016/j.ortho.2015.06.013.

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4

Lagoutte, Priscillia. "La présentation sur ribosome". médecine/sciences 36, n.º 8-9 (agosto de 2020): 717–24. http://dx.doi.org/10.1051/medsci/2020126.

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La présentation sur ribosome (en anglais, ribosome display) est une méthode d’évolution moléculaire et de sélection de banques peptidiques et protéiques. Le ribosome display est réalisé in vitro dans un milieu acellulaire et repose sur la formation d’un complexe ternaire ribonucléoprotéique entre l’ARN, le ribosome et la protéine. Le ribosome display est devenu de nos jours l’une des méthodes de présentation les plus utilisées. Elle a notamment permis le criblage et la sélection de peptides, de protéines, d’échafaudages moléculaires afin d’améliorer leur affinité, leur spécificité, leur activité catalytique ou même leur stabilité. Cette revue présente la mise en œuvre du ribosome display et les applications qui découlent de l’utilisation de cette technologie.

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Daou, Mark, Pascale Habre-Hallage e Elie Zebouni. "In Vitro Evaluation of the Color Stability of Two Different Layering Ceramics after Thermocycling in Coffee = Evaluation In Vitro de la Stabilité de Couleur de Deux Différentes Céramiques de Stratification Après Thermocyclage dans le Café". International Arab Journal of Dentistry 12, n.º 2 (novembro de 2021): 73–80. http://dx.doi.org/10.12816/0060162.

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6

Aloulou, A., D. Puccinelli, J. Sarles, R. Laugier, Y. Leblond e F. Carrière. "O028 Étude comparative in vitro de trois préparations enzymatiques pancréatiques : profils de dissolution, libération d’enzymes actifs et stabilité à pH acide". Nutrition Clinique et Métabolisme 21 (novembro de 2007): 45. http://dx.doi.org/10.1016/s0985-0562(07)78801-5.

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Jolivot, P. A., C. Dunyach-Remy, A. Roussey, A. Jalabert, M. Mallié e S. Hansel-Esteller. "Étude d’activité in vitro et de stabilité de suspensions antifongiques pour bain de bouche : vers une remise en question de pratiques empiriques ?" Pathologie Biologie 60, n.º 6 (dezembro de 2012): 362–68. http://dx.doi.org/10.1016/j.patbio.2012.01.002.

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REZAEI, H., J. GROSCLAUDE, F. EGHIAIAN, T. HAERTLE, M. MARDEN, M. KNOSSOW e P. DEBEY. "Lien entre type génétique et résistance des ovins à la Tremblante : une approche structurale et physico-chimique". INRAE Productions Animales 17, HS (20 de dezembro de 2004): 45–50. http://dx.doi.org/10.20870/productions-animales.2004.17.hs.3626.

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Le polymorphisme génétique de la protéine prion ovine associé à des degrés divers de résistance ou de sensibilité à la tremblante ouvre une voie féconde pour comprendre le lien entre les propriétés structurales de la protéine et le mécanisme du développement de la pathologie. Les travaux menés par les équipes de l’INRA, en collaboration avec d’autres équipes nationales, ont apporté des renseignements inattendus sur la stabilité et la convertibilité des variants naturellement rencontrés dans les troupeaux européens. Les mécanismes, au niveau atomique, sous-tendant ces caractéristiques ont pu être explicités par la détermination cristallographique de la structure tri-dimensionnelle de la protéine ovine, apportant en même temps une première information expérimentale sur la structure de la protéine pathologique. Des formes intermédiaires de repliement, sous forme d’oligomères solubles, ont pu être isolées in vitro, et se sont révélées neurotoxiques, ouvrant de nouvelles pistes de recherche vers les déterminants respectifs de la mort neuronale et de la réplication du prion.

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9

Kitten, Olivier, e Pierre Martineau. "Les formats alternatifs aux anticorps". médecine/sciences 35, n.º 12 (dezembro de 2019): 1092–97. http://dx.doi.org/10.1051/medsci/2019217.

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Les anticorps sont désormais devenus d’une utilisation courante dans un large champ thérapeutique qui n’est plus restreint à la cancérologie et à l’inflammation. Cette explosion du domaine conduit à des besoins nouveaux qui peuvent être mieux remplis par des molécules inspirées mais différentes des anticorps classiques. En particulier, la molécule anticorps a de multiples fonctions qui ne sont pas toujours nécessaires, comme sa capacité à recruter les cellules du système immunitaire, à se lier de façon bivalente à sa cible ou à présenter une demi-vie plasmatique élevée. En revanche, dans la grande majorité des applications, sa remarquable capacité à reconnaître spécifiquement sa cible moléculaire et surtout sa diversité de reconnaissance doivent être conservées. De plus, les anticorps sont des molécules de très haut poids moléculaire, coûteuses à produire et qui présentent des propriétés physicochimiques limitées ne permettant pas leur utilisation dans des milieux agressifs. Finalement, dans certaines applications thérapeutiques, la grande taille de la molécule (environ 150 kDa) peut également limiter sa diffusion dans les tissus et empêcher la reconnaissance de certaines structures moléculaires peu accessibles. Pour répondre à ces limitations, de nombreux formats alternatifs aux anticorps entiers ont été développés au cours de ces vingt dernières années. Les applications couvrent les domaines de la biotechnologie, du diagnostic in vitro et in vivo et de la thérapie. Deux grandes familles de molécules permettent de couvrir ce champ et seront présentées dans cette mini-revue. Une première famille s’appuie sur la diversité naturelle des anticorps mais en en réduisant la taille, comme les fragments d’anticorps classiques (Fab, scFv) ou ceux provenant des camélidés ou des requins (VHH, V-NAR). La deuxième famille a été développée en partant des propriétés finales désirées et notamment la stabilité en milieu extrême et la productivité en système simple et économique de production comme l’utilisation de bactéries et en y greffant des propriétés de liaison comparables aux anticorps par des méthodes d’évolution moléculaire dirigée in vitro. Cette mini-revue se concentrera sur les molécules les plus avancées, mais le domaine est en très forte et rapide expansion. Il faut noter que beaucoup de ces molécules, voire ces approches, sont couvertes par des brevets et sont souvent développées dans le cadre de jeunes sociétés innovantes dont certaines ont déjà été rachetées par de grands groupes de la pharmacie.

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10

Couteau, C., E. Paparis e L. J. M. Coiffard. "Comparaison de produits de protection solaire ayant un statut de cosmétique ou de dispositif médical par détermination de leur efficacité, de leur photo-stabilité et de leur résistance à l’eau grâce à une méthode in vitro". Annales de Dermatologie et de Vénéréologie 143, n.º 2 (fevereiro de 2016): 124–29. http://dx.doi.org/10.1016/j.annder.2015.08.010.

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11

Mejía, Andrea, Víctor Montaño, Andrés Viteri e Ana Armas. "Influencia del ph salival en la estabilidad del color de diferentes resinas fluidas: estudio in vitro". Kiru 16, n.º 3 (30 de junho de 2019): 108–12. http://dx.doi.org/10.24265/kiru.2019.v16n3.02.

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12

Song, Chung-Kil, Soon-Hwa Jung, Ha-Soo Seong, Sun-Hang Cho e Byung-Cheol Shin. "In Vitro Stability of Liposomes Containing Newly Synthesized Glycolipid". Journal of the Korean Chemical Society 51, n.º 1 (20 de fevereiro de 2007): 43–50. http://dx.doi.org/10.5012/jkcs.2007.51.1.043.

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13

Sokolov, I. S., M. K. Tatarintsev, R. Y. Khasanov, A. M. Azieva, E. Yu Makarenko e M. S. Burtsev. "Stability of spontaneous electrical activity of neural networks in vitro". Bulletin of Russian State Medical University, n.º 2 (2016): 42–46. http://dx.doi.org/10.24075/brsmu.2016-02-06.

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14

Gautam, Nitin, Monica Kotwal, Rimsha Ahmed, Anupama Gaur e Sunny Sharma. "Stability of Denture Base Acrylic Resin to Tea, Coffe and Turmeric Solutions: An in Vitro Study". Annals of International Medical and Dental Research 9, n.º 2 (abril de 2023): 46–49. http://dx.doi.org/10.53339/aimdr.2023.9.10.

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Background: The color stability of commercially available denture base acrylic resins (Lucitone-199, DPI and Travelon-HI) was studied in vitro. Material Methods: The specimens were exposed to tea, coffee and turmeric solutions at 37 ± 1 °C. Colour measurement of the specimens from each brand of denture base acrylic resin recorded by spectrophotometer. The specimens were washed under distilled water and dried before measuring the colour on 0, 10, 20 and 30 days of immersion and color differences were calculated. Results: Statistically the colour change was significant between and within the groups of different heat cure denture base acrylic resins. Conclusion: Where as Lucitone-199 heat cure showed the highest colour variation in tea and coffee followed by DPI and Travelon-HI.

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15

Banfi, G., e R. Daverio. "In vitro stability of osteocalcin". Clinical Chemistry 40, n.º 5 (1 de maio de 1994): 833–34. http://dx.doi.org/10.1093/clinchem/40.5.833a.

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16

Haisel, D., P. Hofman, M. Vagner, H. Lipavska, I. Ticha, C. Schafer e V. Capkova. "Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation". Biologia plantarum 44, n.º 3 (1 de setembro de 2001): 321–24. http://dx.doi.org/10.1023/a:1012415004676.

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17

Capron, I., M. Yvon e G. Muller. "In-vitro gastric stability of carrageenan". Food Hydrocolloids 10, n.º 2 (abril de 1996): 239–44. http://dx.doi.org/10.1016/s0268-005x(96)80040-3.

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18

Higashikawa, Fumiko, e Lung-Ji Chang. "Kinetic Analyses of Stability of Simple and Complex Retroviral Vectors". Virology 280, n.º 1 (fevereiro de 2001): 124–31. http://dx.doi.org/10.1006/viro.2000.0743.

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19

Funk, C. J., S. H. Harwood e G. F. Rohrmann. "Differential Stability of Baculovirus Late Transcription Complexes during Initiation and Elongation". Virology 241, n.º 1 (fevereiro de 1998): 131–40. http://dx.doi.org/10.1006/viro.1997.8961.

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20

Kusumanegara, Jayarasti, e Heroe Soebroto. "THE STABILITY OF INTERLOCKING STERNOTOMY TECHNIQUE ON JAVA GOAT STERNUM (CAPRA AEGAGRUS HIRCUS) IN VITRO BASED ON BIOMECHANICAL ANALYSIS". International Journal of Psychosocial Rehabilitation 24, n.º 02 (13 de fevereiro de 2020): 4054–61. http://dx.doi.org/10.37200/ijpr/v24i2/pr200726.

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21

Blackhall, J., A. Fuentes e G. Magnusson. "Genetic Stability of a Porcine Rotavirus RNA Segment during Repeated Plaque Isolation". Virology 225, n.º 1 (novembro de 1996): 181–90. http://dx.doi.org/10.1006/viro.1996.0586.

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22

Rezapkin, Gennady V., Liju Fan, David M. Asher, Mathias R. Fibi, Eugenia M. Dragunsky e Konstantin M. Chumakov. "Mutations in Sabin 2 Strain of Poliovirus and Stability of Attenuation Phenotype". Virology 258, n.º 1 (maio de 1999): 152–60. http://dx.doi.org/10.1006/viro.1999.9718.

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23

Kwak, H. S., S. H. Kwon, J. B. Lee e J. Ahn. "In Vitro Stability of β-galactosidase Microcapsules". Asian-Australasian Journal of Animal Sciences 15, n.º 12 (1 de dezembro de 2002): 1808–12. http://dx.doi.org/10.5713/ajas.2002.1808.

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24

Liu, Lu Jie, Jia Zhu, Bin Wang, Chu Cheng, Yong Jie Du e Min Qi Wang. "In vitro stability evaluation of coated lipase". Asian-Australasian Journal of Animal Sciences 30, n.º 2 (10 de agosto de 2016): 192–97. http://dx.doi.org/10.5713/ajas.16.0370.

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25

Wu, J. T., e J. A. Knight. "In-vitro stability of human alpha-fetoprotein." Clinical Chemistry 31, n.º 10 (1 de outubro de 1985): 1692–97. http://dx.doi.org/10.1093/clinchem/31.10.1692.

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Abstract We assessed the stability of alpha-fetoprotein (AFP) in clinical specimens in the presence and absence of serum and albumin, at different temperatures and concentrations. We find it depends on both AFP concentration and incubation temperature. Dilution of most specimens with either phosphate buffer or phosphate-buffered saline or by immunoelectrodiffusion resulted in some loss of AFP. Attempts to stabilize AFP during either sample dilution or incubation by use of albumin in concentrations up to 1 g/L did not protect it from inactivation unless normal human serum was also included. Frozen AFP solutions were less stable than solutions stored at 4 degrees C. AFP was most stable when lyophilized and stored desiccated. The AFP-inactivation curves were usually nonlinear. Apparently both polymerization and degradation occur simultaneously as AFP loses its activity. Proteolytic enzyme inhibitor and sulfhydryl reagent not only failed to protect it from inactivation, they appeared to speed it.

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26

Faltermeier, A., M. Behr e D. Mussig. "In vitro colour stability of aesthetic brackets". European Journal of Orthodontics 29, n.º 4 (7 de junho de 2007): 354–58. http://dx.doi.org/10.1093/ejo/cjm020.

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27

Murtagh, G. J., M. Dumoulin, D. B. Archer e M. J. Alcocer. "Stability of 2S albumin allergens in vitro". Biochemical Society Transactions 30, n.º 5 (1 de outubro de 2002): A108. http://dx.doi.org/10.1042/bst030a108a.

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Murtagh, G. J., M. Dumoulin, D. B. Archer e M. J. Alcocer. "Stability of 2S albumin allergens in vitro". Biochemical Society Transactions 30, n.º 5 (1 de outubro de 2002): A128. http://dx.doi.org/10.1042/bst030a128c.

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29

Marchbanks, C. R., R. L. Yost e R. L. White. "Cefotaxime stability during in vitro microbiological testing." Antimicrobial Agents and Chemotherapy 31, n.º 9 (1 de setembro de 1987): 1375–78. http://dx.doi.org/10.1128/aac.31.9.1375.

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30

Lucisano, Joseph Y., Jon C. Armour e David A. Gough. "In vitro stability of an oxygen sensor". Analytical Chemistry 59, n.º 5 (março de 1987): 736–39. http://dx.doi.org/10.1021/ac00132a012.

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31

Dellinger, R. P. "Factors Affecting Urinary Myoglobin Stability in Vitro". Yearbook of Critical Care Medicine 2006 (janeiro de 2006): 209. http://dx.doi.org/10.1016/s0734-3299(08)70150-3.

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32

Chen-Levy, Zehava, Mark H. Wener, Bert Toivola, Phyllis Daum, Morayma Reyes e James S. Fine. "Factors Affecting Urinary Myoglobin Stability In Vitro". American Journal of Clinical Pathology 123, n.º 3 (março de 2005): 432–38. http://dx.doi.org/10.1309/9aq62fr265er3e2w.

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Moya, Monica L., Michael Morley, Omaditya Khanna, Emmanuel C. Opara e Eric M. Brey. "Stability of alginate microbead properties in vitro". Journal of Materials Science: Materials in Medicine 23, n.º 4 (16 de fevereiro de 2012): 903–12. http://dx.doi.org/10.1007/s10856-012-4575-9.

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Yang, Hu, e Stephanie T. Lopina. "In vitro enzymatic stability of dendritic peptides". Journal of Biomedical Materials Research Part A 76A, n.º 2 (2005): 398–407. http://dx.doi.org/10.1002/jbm.a.30529.

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35

Chien, Mei-Ling, Eduardo O'Neill e J. Victor Garcia">. "Phosphate Depletion Enhances the Stability of the Amphotropic Murine Leukemia Virus Receptor mRNA". Virology 240, n.º 1 (janeiro de 1998): 109–17. http://dx.doi.org/10.1006/viro.1997.8933.

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Ahn, Anna, Randal J. Schoepp, David Sternberg e Margaret Kielian. "Growth and Stability of a Cholesterol-Independent Semliki Forest Virus Mutant in Mosquitoes". Virology 262, n.º 2 (setembro de 1999): 452–56. http://dx.doi.org/10.1006/viro.1999.9932.

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YILDIZ, Berkan, Sevde Gül BATMAZ, Ayşe DÜNDAR e Çağatay BARUTÇUGİL. "Evaluation of Color Stability of Dental Composites with Smart Chromatic Technology: An in Vitro Study". Turkiye Klinikleri Journal of Dental Sciences 29, n.º 2 (2023): 358–66. http://dx.doi.org/10.5336/dentalsci.2022-93854.

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Lauermann, Vit, Stephen H. Hughes e Keith W. C. Peden. "Maintenance of an Unusual Polypurine Tract in HIV-2: Stability to Passage in Culture". Virology 236, n.º 1 (setembro de 1997): 208–12. http://dx.doi.org/10.1006/viro.1997.8721.

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Dolja, Valerian V., Valery V. Peremyslov, Karen E. Keller, Robert R. Martin e Jin Hong. "Isolation and Stability of Histidine-Tagged Proteins Produced in Plants via Potyvirus Gene Vectors". Virology 252, n.º 1 (dezembro de 1998): 269–74. http://dx.doi.org/10.1006/viro.1998.9458.

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Mindich, Leonard, Xueying Qiao, Shiroh Onodera, Paul Gottlieb e Mikko Frilander. "RNA Structural Requirements for Stability and Minus-Strand Synthesis in the dsRNA Bacteriophage φ6". Virology 202, n.º 1 (julho de 1994): 258–63. http://dx.doi.org/10.1006/viro.1994.1341.

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Rezapkin, Gennady V., Konstantin M. Chumakov, Zhengbin Lu, Yuxin Ran, Eugenia M. Dragunsky e Inessa S. Levenbook. "Microevolution of Sabin 1 Strain in Vitro and Genetic Stability of Oral Poliovirus Vaccine". Virology 202, n.º 1 (julho de 1994): 370–78. http://dx.doi.org/10.1006/viro.1994.1353.

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Zhang, Hong, Xue-Kui Yu, Xing-Ying Lu, Jing-Qiang Zhang e Z. Hong Zhou. "Molecular Interactions and Viral Stability Revealed by Structural Analyses of Chemically Treated Cypovirus Capsids". Virology 298, n.º 1 (junho de 2002): 45–52. http://dx.doi.org/10.1006/viro.2002.1473.

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Ratnapuri, Prima Happy, Fajrina Haitami e Mia Fitriana. "Stabilitas Fisik Sediaan Emulgel Ekstrak Etanol Daging Buah Limpasu (Baccaurea lanceolata (Miq.) Müll. Arg.)". Jurnal Pharmascience 6, n.º 2 (20 de novembro de 2019): 8. http://dx.doi.org/10.20527/jps.v6i2.7345.

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ABSTRAK Ekstrak etanol daging buah limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) telah teruji memiliki aktivitas tabir surya secara in vitro, sehingga diformulasikan dalam bentuk sediaan emulgel dengan variasi konsentrasi (% b/b) ekstrak etanol daging B.lanceolata FI (4%), FII (5%) dan FIII (6%). Sediaan emulgel yang telah dibuat selanjutnya perlu dilakukan uji stabilitas fisik saat penyimpanan. Penelitian ini bertujuan untuk menentukan stabilitas fisik sediaan emulgel ekstrak etanol daging buah B. lanceolata selama penyimpanan. Uji stabilitas fisik dilakukan selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C dengan evaluasi meliputi uji organoleptis, uji pH, uji daya sebar, uji daya lekat dan uji viskositas pada hari ke-0, 7, 14, 21 dan 28. Analisis secara statistik dilakukan dengan software SPSS 21 pada taraf kepercayaan 95%. Hasil penelitian pada formula I, II, dan III dengan variasi konsentrasi ekstrak menunjukkan bahwa penyimpanan selama 28 hari pada suhu tinggi 40°±2°C dan ruang 28°C±2°C tidak mempengaruhi kestabilan pH, viskositas, daya sebar dan daya lekat gel (p>0,050). Kesimpulan dari penelitian ini menunjukkan bahwa sediaan emulgel ekstrak etanol daging B. lanceolata stabil secara fisik selama 28 hari pada suhu tinggi 40°±2°C dan suhu ruang 28°C±2°C. Kata Kunci: Baccaurea lanceolata, ekstrak etanol, emulgel, stabilitas fisik. ABSTRACT Limpasu (Baccaurea lanceolata (Miq.). Müll. Arg.) fructus ethanol extract has been reported as sunscreen activity by in vitro test, so that it could be formulated in sunscreen product with their concentration variances FI (4%), FII (5%) and FIII (6%) (% b/b). The further this preparation needs to be tested for physical stability during storage. This study aimed to determine the emulgel stability physically of B.lanceolata fructus ethanol extract during storage. Physical stability test was performed at high temperature 40°±2°C and room temperature of 28°±2oC during 28 days with evaluation including organoleptic, pH, dispersive, adhesion power and viscosity test on days 0, 7, 14, 21 and 28. Statistical analysis, SPSS 21 software at 95% confidence level. This study results, formula I, II, and III with their concentration variances showed that storage for 28 days at high temperature 40°±2°C and 28°±2° C didn’t affect the pH, viscosity, dispersive, adhesion power stability (p>0,050). The conclusion of this study showed that the emulgel preparation of B. lanceolata fructus ethanol extract were physically stable for 28 days at high temperature of 40°±2°C and room temperature of 28°C±2°C. Keywords: Baccaurea lanceolata, ethanol extract, emulgel, physical stability

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Sella Senthil M, Arun Radhakrishnan, Venugopal M e Gowthamarajan Kuppuswamy. "Regulatory on stability studies for In-vitro diagnostic medical device". International Journal of Research in Pharmaceutical Sciences 10, n.º 4 (16 de outubro de 2019): 3277–85. http://dx.doi.org/10.26452/ijrps.v10i4.1633.

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The stability of an in-vitro diagnostic (IVD) medical device reagent is the ability to maintain the performance characteristics over a defined time interval. The stability studies are performed to demonstrate that the product remains viable under the specified storage condition until the claimed time period. Since, stability of the IVD cannot be directly assessed through accuracy, performance attributes or customer testing; it is the responsibility of the manufacturer to evaluate the performance of the product by identifying critical factors affecting the stability through developing stability plan, stability protocol and conducting real-time stability studies, accelerated stability studies, in-use stability studies and transport simulated stability studies.

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Vaulina, D. D., O. F. Kuznetsova, O. S. Fedorova e R. N. Krasikova. "SYNTHESIS AND IN VITRO STUDY OF FLUORINE-18 LABELLED PYRIDOXINE (VITAMIN B6) STABILITY". International Journal of Applied and Fundamental Research (Международный журнал прикладных и фундаментальных исследований), n.º 12 2022 (2022): 110–16. http://dx.doi.org/10.17513/mjpfi.13493.

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KEWALRAMANI, VINEET N., CECILIA S. PARK, PETER A. GALLOMBARDO e MICHAEL EMERMAN. "Protein Stability Influences Human Immunodeficiency Virus Type 2 Vpr Virion Incorporation and Cell Cycle Effect". Virology 218, n.º 2 (abril de 1996): 326–34. http://dx.doi.org/10.1006/viro.1996.0201.

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McCulloch, Steve. "1053 GENETIC STABILITY OF MICROPROPAGATED PLANTS". HortScience 29, n.º 5 (maio de 1994): 579d—579. http://dx.doi.org/10.21273/hortsci.29.5.579d.

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Briggs Nurseries, Inc. has used micropropagation as method of vegetative propagation for over 20 years. Genetic stability and uniformity of plants that are produced and sold is of the utmost concern to the commercial plant propagator. Genetic stability may be accomplished by ensuring that all shoots formed in vitro are of axillary origin and by reducing shoot proliferation rates through the use of lower cytokinin concentrations in the culture medium. Excision and removal of callus during transfer is also necessary to ensure that shoots develop from axillary buds. Various factors that may influence genetic variability and its frequency of in vitro derived plants will be discussed with an emphasis on how to reduce them. Three sources of variation with tissue culture derived plants will also be reviewed (Swartz, 1991): a) source plant variability, b) genetic changes in vitro, and c) epigenetic or physiological adaptation.

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Nishiya, T., e B. Jain. "Study on in Vitro Stability of Polymerized Liposomes". Artificial Cells, Blood Substitutes, and Biotechnology 24, n.º 1 (janeiro de 1996): 43–50. http://dx.doi.org/10.3109/10731199609117430.

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Dursun, Buse, Ahu Altınkut Uncuoğlu e Yıldız Aydin. "Chromosome stability of in vitro propagated Cucurbita cultivars". Genetics & Applications 3, n.º 3 (24 de dezembro de 2019): 25. http://dx.doi.org/10.31383/ga.vol3iss3pp25-32.

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Cucurbita pepo L. which is a member of Cucurbitaceae family, is a one-year plant with herbaceous stems, broad leaves and superficial scattered roots. Monoic flower structure in the Cucurbitaceae family and the differences in the maturing time of male and female organs in flowers cause an increase in the foreign fertilization rate. Therefore, there may be positive or negative changes in the existing characteristics of the species. Micro-propagation method can be performed in pumpkin species for clonal propagation, but genetic stability after tissue culture is an important consideration. Chromosome number and morphology are primary cytogenetic parameters that must remain stable after in vitro propagation. We analyzed plants of different hybrid pumpkin genotypes cultivated in our country (Ardendo, Angelina, Torpido, Roni, Sena Hanım) cytogenetically in order to determinate their stability level. Cotyledon nodes, nodes, shoot apex, hypocotyl and internode explants were prepared from the 4-week old C. pepo seedlings by making a horizontal slice through the hypocotyl region. Cotyledonary node explants produced multiple shoots and callus regeneration in MS medium in the presence of N6-benzylamino-purine BA 1 mg/L in Torpido genotype. Elongated shoots were excised from shoot clumps and transferred to rooting medium. The best root generation was obtained from Torpido and the most successful explant type for root regeneration was determined in cotyledon nodes as in shoot regeneration and MS medium without plant growth regulator showed the best root regeneration. The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse. The chromosome number and karyotype analysis were determined in control and in vitro propagated Cucurbita pepo L. plants and ploidy levels were confirmed to be 2n = 40.

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Hyman, A. A., e T. J. Mitchison. "Modulation of microtubule stability by kinetochores in vitro." Journal of Cell Biology 110, n.º 5 (1 de maio de 1990): 1607–16. http://dx.doi.org/10.1083/jcb.110.5.1607.

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The interface between kinetochores and microtubules in the mitotic spindle is known to be dynamic. Kinetochore microtubules can both polymerize and depolymerize, and their dynamic behavior is intimately related to chromosome movement. In this paper we investigate the influence of kinetochores on the inherent dynamic behavior of microtubules using an in vitro assay. The dynamics of microtubule plus ends attached to kinetochores are compared to those of free plus ends in the same solution. We show that microtubules attached to kinetochores exhibit the full range of dynamic instability behavior, but at altered transition rates. Surprisingly, we find that kinetochores increase the rate at which microtubule ends transit from growing to shrinking. This result contradicts our previous findings (Mitchison, T. J., and M. W. Kirschner, 1985b) for technical reasons which are discussed. We suggest that catalysis of the growing to shrinking transition by kinetochores may account for selective depolymerization of kinetochore microtubules during anaphase in vivo. We also investigate the effects of a nonhydrolyzable ATP analogue on kinetochore microtubule dynamics. We find that 5' adenylylimido diphosphate induces a rigor state at the kinetochore-microtubule interface, which prevents depolymerization of the microtubule.

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